Rw5G001440

protein ubiquitination

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0008844
Physical Location & Seq
Forward (+)
1179772 .. 1182603
2832 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G001440.1

Sequence Viewer

Length: 1881 bp
ATGCCCAGTTATAAAAGTGTCGCCCGTATTTACAATTTCTTCTTGGAAAAGAAATGGAGTGAGATAAGGATCTTTTACGAGGAAAACAATTTTGATGTGTTCCTGCTTCTTAATGCTTTGGGAGATACGGCTCTTCACCTTGCAGTGTACAGTGAAGACCTGGAATTGGTGAGATCTCTGCTGAAGATAGTGTACGAAGACAATCCTTTTGCCGAAGCTGGTATAAAATTTCCCTCAGAGAATGCATCTGGAAACACACCACTTCATGAGGCAGCTTCAATAGGAAATGTTAAATTGGTAAAGCTGATATTGAGTTATGAAGATGGCTATCATATGCTGACGACAGTGAACAAGAATGGTGAAACACCTTTTTTTATGGCCGCACTTTATGGCCAGACCAATTTGATAAAAAATTTGGTGAACGATCTTAACGACATAGATTATCATATCCTGTCTAATACCTCCACCATGCTTCATGAGGCTATCCGCGGCTATTACTTTGAAACAGCTCTGGAATTGTTAGACCCAAAATACCGCATACCGAAGGATTGGGTGGATTCAGATGGCATGACCTGCTTTCAGCTGCTAGCTGACATTCCCTCTGCTTTCAAGAGTGGATATTCCTTTGACATATTGGAGAAACTGATCTATGACGGCCTTCCAGTTCCAGAAGATAAGGATGAAAGTGAAAACAACAATGTATCTCCGGTCAATCCAAGTCGAAAGACTGACAAAAAGCATGGATTCCCATCAGTCCAAAGTCTAAGAGACACGAAGAAGCAGCACGGCATAGTGGTGAAGCTTACCGAAATTCTGGCTAAAGAAGACAAGTCATGGCAGGGTCCTGCAGATGGTGCTATGCCAAAAGAATATAATAATTCTACTGGTGCTCCTATGCCAAAAATTAATAATAATACTACTGATGGTCAACGTGACACTCCACTTATTGCAGCTGCAAAGAATGGGATCCCTGAGATTGTTGAGGCCATCTTGAAAGTGTTCCCACAGGCCATCGGGCATGAAAACCACAAAAAAGAAAATGTATTTCATGTTGCTGTGCAGAATCGCAGGACAAATGTCTTGAATCTTCTCAAATCTTATAAATTTGACTATTCGAGGCTCATTTGGAAGTTCAATGCAGATGGTGATGGTATTTTGCATAAAGCTGCATACAAGACTGGTATTTCCTCAAGGGAGAGGCCCGGGGAAGCCCTTTGCTTGCAGTCAGATATTCAATGGTTTGAGCGAGTGAGGAAAATGGTGCCTGCACATCACGCTAACCTACAAAATAAGAAAGAATGTACGCCTCAATCTTTGTTCACAAAAGAGCATGAGAAGCTCGTCAAGAAAGGCCAAGAATGGTTAATCAGAACAACAAACTCATGCATTATAGTTGCAGTTCTTATTGCAACTGTTGCTTTTACAAGTATCTACACCGTGCCTGGCGGTTCTGATGCAAAGACAGGCAAGCCGTTGCTCCTCAACACAACTGCATTCACGGTCTTCACAGCATCTGATGTGGCATCTCTTTGTTTTGCCTTCACCTCAGTGGTGGTGTTTCTTTCCATCACTACATCAAAAATGAATGAGAAAGATTTCCGGATGTCTCTGCCTCTAAAGCTTGTTTTGGGGCTCAGCATGCTGTTTTTTTCAGTGGCAGCATTGATGATTGGTTTTGCTGCTACTCTTGTCATGACAATAAGGCACAGGCTACATCAGGCTGCTGCCCCCATCATTGCCATTGCTTGTTTTCCTGTCGCCTTTTTCCTCTTCCTCCAGCTTCGTCTCTATACCAATATTACTTGGTTTACCATAAAGGACTTGCTGCGCAGCCTCATTGATATTATTCCGAAAAAAATGTCAGCCTCGATCATAGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 PGG

Protein Analysis

626

Amino Acids

70.33

Weight (kDa)

8.01

Isoelectric Point (pI)

35.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 36 - 106 9.4e-09 Ankyrin repeats (3 copies)
Ank_2 PF12796 304 - 371 5.2e-06 Ankyrin repeats (3 copies)
PGG PF13962 453 - 565 1.4e-22 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 12, 1101
Acc16I TGCGCA 1 cut(s) 1829
Acc36I ACCTGC 1 cut(s) 581
AccB1I GGYRCC 1 cut(s) 1261
AccII CGCG 1 cut(s) 489
AccIII TCCGGA 1 cut(s) 1599
AciI CCGC 5 cut(s) 381, 487, 489, 535, 1446
AclWI GGATC 3 cut(s) 77, 961, 974
AcoI YGGCCR 2 cut(s) 378, 391
AcsI RAATTY 4 cut(s) 227, 412, 810, 1103
AcuI CTGAAG 1 cut(s) 203
AfaI GTAC 3 cut(s) 149, 194, 1303
AfiI CCNNNNNNNGG 2 cut(s) 166, 851
AgsI TTSAA 7 cut(s) 279, 503, 610, 994, 1084, 1135, 1235
AjnI CCWGG 2 cut(s) 159, 1441
AleI CACNNNNGTG 1 cut(s) 1547
Alw21I GWGCWC 1 cut(s) 892
Alw26I GTCTC 3 cut(s) 762, 1611, 1790
AlwI GGATC 3 cut(s) 77, 961, 974
AlwNI CAGNNNCTG 1 cut(s) 1514
Ama87I CYCGRG 1 cut(s) 1202
Aor13HI TCCGGA 1 cut(s) 1599
AoxI GGCC 7 cut(s) 378, 391, 655, 984, 1008, 1199, 1351
ApoI RAATTY 4 cut(s) 227, 412, 810, 1103
ArsI GACNNNNNNTTYG 4 cut(s) 191, 223, 1844, 1876
AseI ATTAAT 1 cut(s) 906
Asp700I GAANNNNTTC 2 cut(s) 998, 1595
AspLEI GCGC 1 cut(s) 1830
AspS9I GGNCC 2 cut(s) 842, 1200
AsuC2I CCSGG 2 cut(s) 1203, 1204
AsuHPI GGTGA 7 cut(s) 128, 181, 371, 430, 808, 1157, 1534
AsuNHI GCTAGC 1 cut(s) 586
AvaI CYCGRG 1 cut(s) 1202
AvaII GGWCC 1 cut(s) 842
BalI TGGCCA 1 cut(s) 393
BamHI GGATCC 1 cut(s) 966
BanI GGYRCC 1 cut(s) 1261
BanII GRGCYC 1 cut(s) 1635
BarI GAAGNNNNNNTAC 2 cut(s) 176, 208
BbsI GAAGAC 4 cut(s) 162, 204, 831, 1495
Bbv12I GWGCWC 1 cut(s) 892
BceAI ACGGC 4 cut(s) 144, 670, 802, 1456
BciT130I CCWGG 2 cut(s) 161, 1443
BcnI CCSGG 2 cut(s) 1203, 1204
BcoDI GTCTC 3 cut(s) 762, 1611, 1790
BfaI CTAG 2 cut(s) 587, 1879
BfmI CTRYAG 1 cut(s) 846
BfuAI ACCTGC 1 cut(s) 581
BglII AGATCT 1 cut(s) 173
BlpI GCTNAGC 1 cut(s) 1634
Bme1390I CCNGG 4 cut(s) 161, 1203, 1204, 1443
Bme18I GGWCC 1 cut(s) 842
BmeT110I CYCGRG 1 cut(s) 1202
BmgT120I GGNCC 2 cut(s) 842, 1200
BmiI GGNNCC 3 cut(s) 843, 968, 1263
BmrFI CCNGG 4 cut(s) 161, 1203, 1204, 1443
BmsI GCATC 4 cut(s) 254, 1444, 1520, 1532
BmtI GCTAGC 1 cut(s) 590
BoxI GACNNNNGTC 1 cut(s) 1076
BpiI GAAGAC 4 cut(s) 162, 204, 831, 1495
BpmI CTGGAG 1 cut(s) 1760
Bpu1102I GCTNAGC 1 cut(s) 1634
BpuEI CTTGAG 1 cut(s) 1174
BpuMI CCSGG 2 cut(s) 1203, 1204
BsaBI GATNNNNATC 3 cut(s) 68, 327, 748
BsaJI CCNNGG 3 cut(s) 487, 1202, 1203
BsaWI WCCGGW 2 cut(s) 706, 1599
BsaXI ACNNNNNCTCC 2 cut(s) 874, 904
Bsc4I CCNNNNNNNGG 2 cut(s) 166, 851
Bse1I ACTGG 4 cut(s) 6, 662, 889, 1183
Bse3DI GCAATG 2 cut(s) 1734, 1740
Bse8I GATNNNNATC 3 cut(s) 68, 327, 748
BseAI TCCGGA 1 cut(s) 1599
BseBI CCWGG 2 cut(s) 161, 1443
BseDI CCNNGG 3 cut(s) 487, 1202, 1203
BseGI GGATG 2 cut(s) 685, 1608
BseJI GATNNNNATC 3 cut(s) 68, 327, 748
BseLI CCNNNNNNNGG 2 cut(s) 166, 851
BseMI GCAATG 2 cut(s) 1734, 1740
BseMII CTCAG 4 cut(s) 249, 963, 1560, 1648
BseNI ACTGG 4 cut(s) 6, 662, 889, 1183
BseRI GAGGAG 1 cut(s) 1469
BsgI GTGCAG 2 cut(s) 1079, 1251
Bsh1236I CGCG 1 cut(s) 489
BshFI GGCC 7 cut(s) 380, 393, 657, 986, 1010, 1201, 1353
BshNI GGYRCC 1 cut(s) 1261
BsiHKAI GWGCWC 1 cut(s) 892
BsiHKCI CYCGRG 1 cut(s) 1202
BsiSI CCGG 3 cut(s) 707, 1203, 1600
BslI CCNNNNNNNGG 2 cut(s) 166, 851
BsmAI GTCTC 3 cut(s) 762, 1611, 1790
BsmBI CGTCTC 1 cut(s) 1790
BsmI GAATGC 2 cut(s) 247, 1493
BsnI GGCC 7 cut(s) 380, 393, 657, 986, 1010, 1201, 1353
BsoBI CYCGRG 1 cut(s) 1202
Bsp1286I GDGCHC 2 cut(s) 892, 1635
Bsp13I TCCGGA 1 cut(s) 1599
Bsp1407I TGTACA 1 cut(s) 147
Bsp143I GATC 6 cut(s) 69, 173, 424, 645, 966, 1869
Bsp1720I GCTNAGC 1 cut(s) 1634
BspACI CCGC 5 cut(s) 381, 487, 489, 535, 1446
BspANI GGCC 7 cut(s) 380, 393, 657, 986, 1010, 1201, 1353
BspCNI CTCAG 4 cut(s) 248, 964, 1559, 1647
BspEI TCCGGA 1 cut(s) 1599
BspFNI CGCG 1 cut(s) 489
BspHI TCATGA 3 cut(s) 265, 475, 1692
BspLI GGNNCC 3 cut(s) 843, 968, 1263
BspMAI CTGCAG 1 cut(s) 850
BspMI ACCTGC 1 cut(s) 581
BspOI GCTAGC 1 cut(s) 590
BspPI GGATC 3 cut(s) 77, 961, 974
BspQI GCTCTTC 1 cut(s) 138
BspT107I GGYRCC 1 cut(s) 1261
BsrDI GCAATG 2 cut(s) 1734, 1740
BsrGI TGTACA 1 cut(s) 147
BsrI ACTGG 4 cut(s) 6, 662, 889, 1183
BssECI CCNNGG 3 cut(s) 487, 1202, 1203
BssMI GATC 6 cut(s) 69, 173, 424, 645, 966, 1869
Bst2UI CCWGG 2 cut(s) 161, 1443
Bst4CI ACNGT 5 cut(s) 152, 346, 1414, 1438, 1501
Bst6I CTCTTC 2 cut(s) 138, 1775
BstAPI GCANNNNNTGC 3 cut(s) 573, 854, 1415
BstAUI TGTACA 1 cut(s) 147
BstC8I GCNNGC 5 cut(s) 588, 1220, 1266, 1469, 1640
BstDEI CTNAG 5 cut(s) 235, 764, 972, 1546, 1634
BstDSI CCRYGG 1 cut(s) 487
BstF5I GGATG 2 cut(s) 685, 1608
BstFNI CGCG 1 cut(s) 489
BstHHI GCGC 1 cut(s) 1830
BstKTI GATC 6 cut(s) 72, 176, 427, 648, 969, 1872
BstMAI GTCTC 3 cut(s) 762, 1611, 1790
BstMBI GATC 6 cut(s) 69, 173, 424, 645, 966, 1869
BstMWI GCNNNNNNNGC 7 cut(s) 573, 854, 1274, 1336, 1415, 1618, 1639
BstNI CCWGG 2 cut(s) 161, 1443
BstNSI RCATGY 1 cut(s) 1642
BstPAI GACNNNNGTC 1 cut(s) 1076
BstSCI CCNGG 4 cut(s) 159, 1201, 1202, 1441
BstSFI CTRYAG 1 cut(s) 846
BstUI CGCG 1 cut(s) 489
BstV2I GAAGAC 4 cut(s) 162, 204, 831, 1495
BstX2I RGATCY 3 cut(s) 69, 173, 966
BstYI RGATCY 3 cut(s) 69, 173, 966
BsuRI GGCC 7 cut(s) 380, 393, 657, 986, 1010, 1201, 1353
BtgI CCRYGG 1 cut(s) 487
BtsCI GGATG 2 cut(s) 685, 1608
BtsI GCAGTG 1 cut(s) 150
BtsIMutI CAGTG 5 cut(s) 150, 157, 351, 1554, 1659
BveI ACCTGC 1 cut(s) 581
Cac8I GCNNGC 5 cut(s) 588, 1220, 1266, 1469, 1640
CaiI CAGNNNCTG 1 cut(s) 1514
CciI TCATGA 3 cut(s) 265, 475, 1692
CfoI GCGC 1 cut(s) 1830
Cfr13I GGNCC 2 cut(s) 842, 1200
Cfr42I CCGCGG 1 cut(s) 490
Cfr9I CCCGGG 1 cut(s) 1202
Csp6I GTAC 3 cut(s) 148, 193, 1302
CviQI GTAC 3 cut(s) 148, 193, 1302
DdeI CTNAG 5 cut(s) 235, 764, 972, 1546, 1634
DpnI GATC 6 cut(s) 71, 175, 426, 647, 968, 1871
DpnII GATC 6 cut(s) 69, 173, 424, 645, 966, 1869
EaeI YGGCCR 2 cut(s) 378, 391
Eam1104I CTCTTC 2 cut(s) 138, 1775
EarI CTCTTC 2 cut(s) 138, 1775
Eco24I GRGCYC 1 cut(s) 1635
Eco47I GGWCC 1 cut(s) 842
Eco57I CTGAAG 1 cut(s) 203
Eco88I CYCGRG 1 cut(s) 1202
EcoO109I RGGNCCY 1 cut(s) 842
EcoRII CCWGG 2 cut(s) 159, 1441
EcoT22I ATGCAT 2 cut(s) 247, 1388
EcoT38I GRGCYC 1 cut(s) 1635
Esp3I CGTCTC 1 cut(s) 1790
FauNDI CATATG 1 cut(s) 333
FokI GGATG 2 cut(s) 692, 1615
FriOI GRGCYC 1 cut(s) 1635
FspBI CTAG 2 cut(s) 587, 1879
FspI TGCGCA 1 cut(s) 1829
GlaI GCGC 1 cut(s) 1829
GsuI CTGGAG 1 cut(s) 1760
HaeIII GGCC 7 cut(s) 380, 393, 657, 986, 1010, 1201, 1353
HapII CCGG 3 cut(s) 707, 1203, 1600
HhaI GCGC 1 cut(s) 1830
Hin6I GCGC 1 cut(s) 1828
HinP1I GCGC 1 cut(s) 1828
HincII GTYRAC 1 cut(s) 929
HindII GTYRAC 1 cut(s) 929
HindIII AAGCTT 2 cut(s) 800, 1619
HinfI GANTC 4 cut(s) 557, 744, 1063, 1084
HpaII CCGG 3 cut(s) 707, 1203, 1600
HphI GGTGA 7 cut(s) 128, 181, 371, 430, 808, 1157, 1534
Hpy166II GTNNAC 7 cut(s) 148, 193, 349, 421, 929, 1320, 1809
Hpy188I TCNGA 7 cut(s) 238, 562, 1228, 1370, 1453, 1516, 1851
Hpy8I GTNNAC 7 cut(s) 148, 193, 349, 421, 929, 1320, 1809
HpyAV CCTTC 3 cut(s) 538, 668, 1549
HpyCH4III ACNGT 5 cut(s) 152, 346, 1414, 1438, 1501
HpyCH4IV ACGT 1 cut(s) 931
HpyF10VI GCNNNNNNNGC 7 cut(s) 573, 854, 1274, 1336, 1415, 1618, 1639
HpyF3I CTNAG 5 cut(s) 235, 764, 972, 1546, 1634
HpySE526I ACGT 1 cut(s) 931
HspAI GCGC 1 cut(s) 1828
Kpn2I TCCGGA 1 cut(s) 1599
KspI CCGCGG 1 cut(s) 490
Kzo9I GATC 6 cut(s) 69, 173, 424, 645, 966, 1869
LguI GCTCTTC 1 cut(s) 138
LmnI GCTCC 2 cut(s) 895, 1482
LweI GCATC 4 cut(s) 254, 1444, 1520, 1532
MaeI CTAG 2 cut(s) 587, 1879
MaeII ACGT 1 cut(s) 931
MaeIII GTNAC 1 cut(s) 932
MalI GATC 6 cut(s) 71, 175, 426, 647, 968, 1871
MboI GATC 6 cut(s) 69, 173, 424, 645, 966, 1869
MflI RGATCY 3 cut(s) 69, 173, 966
MhlI GDGCHC 2 cut(s) 892, 1635
MlsI TGGCCA 1 cut(s) 393
MluNI TGGCCA 1 cut(s) 393
Mox20I TGGCCA 1 cut(s) 393
Mph1103I ATGCAT 2 cut(s) 247, 1388
MroI TCCGGA 1 cut(s) 1599
MroXI GAANNNNTTC 2 cut(s) 998, 1595
MscI TGGCCA 1 cut(s) 393
MseI TTAA 5 cut(s) 111, 291, 429, 906, 1364
MslI CAYNNNNRTG 2 cut(s) 794, 1547
Msp20I TGGCCA 1 cut(s) 393
MspA1I CMGCKG 3 cut(s) 489, 583, 953
MspI CCGG 3 cut(s) 707, 1203, 1600
MspR9I CCNGG 4 cut(s) 161, 1203, 1204, 1443
Mva1269I GAATGC 2 cut(s) 247, 1493
MvaI CCWGG 2 cut(s) 161, 1443
MvnI CGCG 1 cut(s) 489
MwoI GCNNNNNNNGC 7 cut(s) 573, 854, 1274, 1336, 1415, 1618, 1639
NciI CCSGG 2 cut(s) 1203, 1204
NdeI CATATG 1 cut(s) 333
NdeII GATC 6 cut(s) 69, 173, 424, 645, 966, 1869
NheI GCTAGC 1 cut(s) 586
NlaIV GGNNCC 3 cut(s) 843, 968, 1263
NmuCI GTSAC 1 cut(s) 932
NsbI TGCGCA 1 cut(s) 1829
NsiI ATGCAT 2 cut(s) 247, 1388
NspI RCATGY 1 cut(s) 1642
OliI CACNNNNGTG 1 cut(s) 1547
PaeI GCATGC 1 cut(s) 1642
PagI TCATGA 3 cut(s) 265, 475, 1692
PciSI GCTCTTC 1 cut(s) 138
PcsI WCGNNNNNNNCGW 1 cut(s) 429
PctI GAATGC 2 cut(s) 247, 1493
PdmI GAANNNNTTC 2 cut(s) 998, 1595
PfeI GAWTC 4 cut(s) 557, 744, 1063, 1084
PpuMI RGGWCCY 1 cut(s) 842
PshAI GACNNNNGTC 1 cut(s) 1076
PshBI ATTAAT 1 cut(s) 906
PsiI TTATAA 2 cut(s) 12, 1101
Psp5II RGGWCCY 1 cut(s) 842
Psp6I CCWGG 2 cut(s) 159, 1441
PspGI CCWGG 2 cut(s) 159, 1441
PspN4I GGNNCC 3 cut(s) 843, 968, 1263
PspPI GGNCC 2 cut(s) 842, 1200
PspPPI RGGWCCY 1 cut(s) 842
PstI CTGCAG 1 cut(s) 850
PstNI CAGNNNCTG 1 cut(s) 1514
PsuI RGATCY 3 cut(s) 69, 173, 966
PvuII CAGCTG 2 cut(s) 583, 953
RsaI GTAC 3 cut(s) 149, 194, 1303
RsaNI GTAC 3 cut(s) 148, 193, 1302
RseI CAYNNNNRTG 2 cut(s) 794, 1547
SacII CCGCGG 1 cut(s) 490
SapI GCTCTTC 1 cut(s) 138
SaqAI TTAA 5 cut(s) 111, 291, 429, 906, 1364
Sau3AI GATC 6 cut(s) 69, 173, 424, 645, 966, 1869
Sau96I GGNCC 2 cut(s) 842, 1200
ScrFI CCNGG 4 cut(s) 161, 1203, 1204, 1443
SduI GDGCHC 2 cut(s) 892, 1635
SfaNI GCATC 4 cut(s) 254, 1444, 1520, 1532
SfcI CTRYAG 1 cut(s) 846
Sfr303I CCGCGG 1 cut(s) 490
SgrBI CCGCGG 1 cut(s) 490
SinI GGWCC 1 cut(s) 842
SmaI CCCGGG 1 cut(s) 1204
SmiMI CAYNNNNRTG 2 cut(s) 794, 1547
SmlI CTYRAG 1 cut(s) 1189
SmoI CTYRAG 1 cut(s) 1189
SphI GCATGC 1 cut(s) 1642
SsiI CCGC 5 cut(s) 381, 487, 489, 535, 1446
SspI AATATT 1 cut(s) 1798
SspMI CTAG 2 cut(s) 587, 1879
StyD4I CCNGG 4 cut(s) 159, 1201, 1202, 1441
TaaI ACNGT 5 cut(s) 152, 346, 1414, 1438, 1501
TaiI ACGT 1 cut(s) 934
TaqI TCGA 3 cut(s) 721, 1115, 1868
TatI WGTACW 1 cut(s) 147
TauI GCSGC 2 cut(s) 383, 492
TfiI GAWTC 4 cut(s) 557, 744, 1063, 1084
Tru1I TTAA 5 cut(s) 111, 291, 429, 906, 1364
Tru9I TTAA 5 cut(s) 111, 291, 429, 906, 1364
TscAI CASTG 5 cut(s) 150, 157, 351, 1554, 1659
TseFI GTSAC 1 cut(s) 932
Tsp45I GTSAC 1 cut(s) 932
TspDTI ATGAA 7 cut(s) 254, 333, 464, 696, 1035, 1037, 1598
TspMI CCCGGG 1 cut(s) 1202
TspRI CASTG 5 cut(s) 150, 157, 351, 1554, 1659
VpaK11BI GGWCC 1 cut(s) 842
VspI ATTAAT 1 cut(s) 906
XapI RAATTY 4 cut(s) 227, 412, 810, 1103
XceI RCATGY 1 cut(s) 1642
XmaI CCCGGG 1 cut(s) 1202
XmnI GAANNNNTTC 2 cut(s) 998, 1595
XspI CTAG 2 cut(s) 587, 1879
Zsp2I ATGCAT 2 cut(s) 247, 1388
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.