Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr5

Orthogroup ID

OG0008844

Physical Location & Seq

Genomic Coordinates Forward (+)

1237827 .. 1240829

Gene Structure 3003 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw5G001470.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1971 bp

ATGTGTTGCTCGGAGGATCTGAATCTGGCTCGCCGTAGGAATGATGTCACTGATGTAGGAGGCGTAATTGGACGATTAAGATGGAAAAGAAAAGTGTTCCAGGATACCGCAATTCATGTTGCAGTGTACAGTAACAACAAAGAACTGGTGGAGTCTCTGCTTGAGAAAGTGTATGGAGATGATCTCAGAGATTACTTTTTCGGAGGGCCAGGAACATTTCTGAAACAGAATGCATTTGGAAATACACCTCTTCACGAGGCAGCTTCGATAGGAAATGTCGACATGGTGAAGATCATAATGCAATATGATCGGAAGCAACTGAATACTAAAAACAAGAGAGGTGAAACACCCTTTTTTACTGCCGCAGTATATGGCAATACCAATGTGATCAAGCATTTGGCGTCGGAGATCAAGGAAGACGATGAGAGGCTATATCACACCTCATCTCCCGGCTCCACGGTGCTTCATGAAGCTATTCGCGGCTATTACTTTGAAACAGCTTTGGAGTTAGTAAAACCAGAATACCACATACAGAAAGATCAGAAGGATTCAGATCACATGACCTGTTTTCAGCTGCTAGCTAACATTCCATCTGCTTTTGAGAGTGGATATTCCTTTGGCATATTGGAGAAACTGATCTATCTTTGCCTTCCGGTAGATGATTATAGCAAAAACAAGGACGACAACAGTGTATCTCAGGTCCCTCCAACTCCAAATACAGATTCGGAGCGTGGCGATCACAACAAAGTCAAGGATGGAAACAAAAATGTATCTCATTTCCCTCGAAGTCCAAACACAGCCTTAAGGAAGATCACAGCAAAAATGTACGTCATCCTTTGGAGCATCATTGCTAAAGGGTTCCCATCTATTAAAGATCTGTGGGACACAAAGAAGAAGCACGGCATAGTGGTGGAGCTTTCAAAAATTCTGGCTGGAGGAGACAAGTCATGGCAGGATAACAACACTGATGGTCAAAAGGACACTCCACTTCTGGCTGCTGCTAGGAATGGGATTATAGAAATCATTGAGGCCATTTTTAATCTGTTCCCGCAGGCCATCGAGCATGAAAACCACAAAAGAGAGAACATATTTCATGTTGTTGTGGAGCATCGGAGGAGACAGCTTTTGGAATATCTTCTCCGTACTTGTCATGTTTCATATCCGAGGCTCCTGTATAAAATCAATCAAGACGGTGACGGCATTTTGCATAAAGCAGCATATAAGAGCCACTATTCCTCAAGGGAGAGGCCAGGGGAAGCCCTTCGCTTGCAGTCAGATATTCAGTGGTTTGAGCGAGTGAAGCAATTGGTGCCTCTACATCACATCAACCATCTTAATCATCATGACAATTTGACACCACATGCTTTGTTCACAAAAGAGCATAAAGAACTGGTGAAGAATGGCCAAGAATGGTTAATCAGAACAACAAACTCTTGCATTATAGTTGCAGTTCTTATTGCAACGGTTGCTTTTACAAGCATCTACACCTTGCCTGGAGGTTCGGATCCAAAGACAGGCAAACCCTTGCTCCTAACCAAAACCTCATTCACTGTCTTCACAGCATCTGATGTGGCATCCCTTTGTTTTGCCTTCACCTCCGTGGTCGTGTTTCTTTCCATCAGCACATCGAAAATGAATGAGAGAGATTTCCGGAGGGCTCTGCCATTGAAGCTTGTGTTGGGGCTAAGCATGCTGTTTTGGTCTGTGGCAGCATTGATGGTTGGTTTTGCTGCTACTCTTGACATGACAATAAAACAAAGGCTGCATCAGGCTACTGCTCCGATCATTACCATTGCTTGTTTTCCAGTTGCCTTTTTCCTCCTTCTGCAGCTTCCTCTCTATTTCAATATTACTTGGTTTACTATAAAGGACTTGCTGCAAACCCTAGTTGATTGTATTCCTCAACGAAAATGTAAGAGCTCTAGCTGCATTATGCGCTATCAAGAGAGCTTTGGTCATTCATTGTGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ank_2 PGG

Protein Analysis

656

Amino Acids

74.49

Weight (kDa)

8.82

Isoelectric Point (pI)

42.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ank_2	PF12796	33 - 109	4.2e-08	Ankyrin repeats (3 copies)
Ank_4	PF13637	38 - 97	1e-06	Ankyrin repeats (many copies)
PGG	PF13962	470 - 580	4.4e-23	Domain of unknown function

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008844)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g01320
rosa_chinensis	RchiOBHm_Chr5g0001861 RchiOBHm_Chr5g0001871 RchiOBHm_Chr5g0001931
rosa_laevigata	RLG00000030979 RLG00000030982 RLG00000030983
rosa_multiflora	Rmu_sc0000378.1_g000006 Rmu_sc0000378.1_g000016 Rmu_sc0006008.1_g000003
rosa_roxburghii	Rroxscaffold_1G00071330 Rroxscaffold_1G00074400 Rroxscaffold_1G00074490
rosa_rugosa	Rorug04G0393600 Rorug04G0393600 Rorug04G0393900
rosa_samantha	Rh5AG014900 Rh5BG018300 Rh5CG016000 Rh5DG016400 Rh5DG016900
rosa_wichuraiana	Rw5G001440 Rw5G001470

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	1309
AccI	GTMKAC	1 cut(s)	279
AccII	CGCG	1 cut(s)	480
AccIII	TCCGGA	1 cut(s)	1650
AciI	CCGC	4 cut(s)	108, 363, 480, 1049
AclWI	GGATC	3 cut(s)	24, 1499, 1512
AcoI	YGGCCR	1 cut(s)	1402
AcsI	RAATTY	1 cut(s)	924
AcyI	GRCGYC	1 cut(s)	401
AfaI	GTAC	3 cut(s)	128, 827, 1144
AfiI	CCNNNNNNNGG	1 cut(s)	1514
AflII	CTTAAG	1 cut(s)	802
AgsI	TTSAA	4 cut(s)	494, 921, 1669, 1846
AjnI	CCWGG	4 cut(s)	99, 208, 1249, 1492
AjuI	GAANNNNNNNTTGG	6 cut(s)	706, 738, 1529, 1561, 1661, 1693
AleI	CACNNNNGTG	1 cut(s)	1598
Alw21I	GWGCWC	1 cut(s)	1922
Alw26I	GTCTC	3 cut(s)	159, 933, 1111
AlwI	GGATC	3 cut(s)	24, 1499, 1512
AlwNI	CAGNNNCTG	1 cut(s)	1565
Aor13HI	TCCGGA	1 cut(s)	1650
AoxI	GGCC	5 cut(s)	206, 1029, 1053, 1247, 1402
ApoI	RAATTY	1 cut(s)	924
Asp700I	GAANNNNTTC	3 cut(s)	474, 1134, 1260
AspLEI	GCGC	1 cut(s)	1938
AspS9I	GGNCC	2 cut(s)	206, 700
AsuC2I	CCSGG	1 cut(s)	450
AsuHPI	GGTGA	5 cut(s)	298, 353, 1205, 1405, 1585
AsuNHI	GCTAGC	1 cut(s)	577
AvaII	GGWCC	1 cut(s)	700
BalI	TGGCCA	1 cut(s)	1404
BamHI	GGATCC	1 cut(s)	1504
BanI	GGYRCC	1 cut(s)	1309
BanII	GRGCYC	2 cut(s)	1660, 1922
BauI	CACGAG	1 cut(s)	254
BbsI	GAAGAC	2 cut(s)	423, 1546
Bbv12I	GWGCWC	1 cut(s)	1922
BccI	CCATC	9 cut(s)	75, 598, 749, 871, 962, 1064, 1338, 1625, 1711
BceAI	ACGGC	3 cut(s)	18, 916, 1213
BcgI	CGANNNNNNTGC	2 cut(s)	290, 324
BciT130I	CCWGG	4 cut(s)	101, 210, 1251, 1494
BciVI	GTATCC	1 cut(s)	97
BclI	TGATCA	1 cut(s)	387
BcnI	CCSGG	1 cut(s)	450
BcoDI	GTCTC	3 cut(s)	159, 933, 1111
BfaI	CTAG	4 cut(s)	578, 1002, 1886, 1923
BfmI	CTRYAG	1 cut(s)	1826
BfrI	CTTAAG	1 cut(s)	802
BfuI	GTATCC	1 cut(s)	97
BglII	AGATCT	1 cut(s)	874
BlpI	GCTNAGC	1 cut(s)	1685
Bme1390I	CCNGG	5 cut(s)	101, 210, 450, 1251, 1494
Bme18I	GGWCC	1 cut(s)	700
BmgT120I	GGNCC	2 cut(s)	206, 700
BmiI	GGNNCC	6 cut(s)	454, 702, 860, 1169, 1311, 1506
BmrFI	CCNGG	5 cut(s)	101, 210, 450, 1251, 1494
BmsI	GCATC	6 cut(s)	852, 1117, 1488, 1571, 1583, 1774
BmtI	GCTAGC	1 cut(s)	581
BpiI	GAAGAC	2 cut(s)	423, 1546
BplI	GAGNNNNNCTC	2 cut(s)	168, 200
BpmI	CTGGAG	2 cut(s)	954, 1515
Bpu1102I	GCTNAGC	1 cut(s)	1685
BpuEI	CTTGAG	2 cut(s)	182, 1222
BpuMI	CCSGG	1 cut(s)	450
BsaBI	GATNNNNATC	2 cut(s)	21, 552
BsaHI	GRCGYC	1 cut(s)	401
BsaJI	CCNNGG	4 cut(s)	456, 1163, 1250, 1599
BsaWI	WCCGGW	2 cut(s)	652, 1650
BsaXI	ACNNNNNCTCC	4 cut(s)	430, 460, 1512, 1542
Bsc4I	CCNNNNNNNGG	1 cut(s)	1514
Bse1I	ACTGG	3 cut(s)	150, 1395, 1805
Bse3DI	GCAATG	2 cut(s)	846, 1791
Bse8I	GATNNNNATC	2 cut(s)	21, 552
BseAI	TCCGGA	1 cut(s)	1650
BseBI	CCWGG	4 cut(s)	101, 210, 1251, 1494
BseDI	CCNNGG	4 cut(s)	456, 1163, 1250, 1599
BseGI	GGATG	3 cut(s)	760, 831, 1574
BseJI	GATNNNNATC	2 cut(s)	21, 552
BseLI	CCNNNNNNNGG	1 cut(s)	1514
BseMI	GCAATG	2 cut(s)	846, 1791
BseMII	CTCAG	2 cut(s)	199, 710
BseNI	ACTGG	3 cut(s)	150, 1395, 1805
BseRI	GAGGAG	2 cut(s)	951, 1129
Bsh1236I	CGCG	1 cut(s)	480
BshFI	GGCC	5 cut(s)	208, 1031, 1055, 1249, 1404
BshNI	GGYRCC	1 cut(s)	1309
BsiHKAI	GWGCWC	1 cut(s)	1922
BsiSI	CCGG	3 cut(s)	450, 653, 1651
BslFI	GGGAC	2 cut(s)	686, 896
BslI	CCNNNNNNNGG	1 cut(s)	1514
BsmAI	GTCTC	3 cut(s)	159, 933, 1111
BsmFI	GGGAC	2 cut(s)	686, 896
BsmI	GAATGC	1 cut(s)	235
BsnI	GGCC	5 cut(s)	208, 1031, 1055, 1249, 1404
Bsp1286I	GDGCHC	2 cut(s)	1660, 1922
Bsp13I	TCCGGA	1 cut(s)	1650
Bsp1407I	TGTACA	1 cut(s)	126
Bsp1720I	GCTNAGC	1 cut(s)	1685
BspACI	CCGC	4 cut(s)	108, 363, 480, 1049
BspANI	GGCC	5 cut(s)	208, 1031, 1055, 1249, 1404
BspCNI	CTCAG	2 cut(s)	198, 709
BspEI	TCCGGA	1 cut(s)	1650
BspFNI	CGCG	1 cut(s)	480
BspHI	TCATGA	2 cut(s)	466, 1342
BspLI	GGNNCC	6 cut(s)	454, 702, 860, 1169, 1311, 1506
BspMAI	CTGCAG	1 cut(s)	1830
BspOI	GCTAGC	1 cut(s)	581
BspPI	GGATC	3 cut(s)	24, 1499, 1512
BspT107I	GGYRCC	1 cut(s)	1309
BspTI	CTTAAG	1 cut(s)	802
BsrDI	GCAATG	2 cut(s)	846, 1791
BsrGI	TGTACA	1 cut(s)	126
BsrI	ACTGG	3 cut(s)	150, 1395, 1805
BssECI	CCNNGG	4 cut(s)	456, 1163, 1250, 1599
BssNI	GRCGYC	1 cut(s)	401
BssSI	CACGAG	1 cut(s)	254
Bst2BI	CACGAG	1 cut(s)	254
Bst2UI	CCWGG	4 cut(s)	101, 210, 1251, 1494
Bst4CI	ACNGT	6 cut(s)	131, 460, 689, 1193, 1465, 1552
Bst6I	CTCTTC	1 cut(s)	255
BstACI	GRCGYC	1 cut(s)	401
BstAFI	CTTAAG	1 cut(s)	802
BstAPI	GCANNNNNTGC	2 cut(s)	1309, 1466
BstAUI	TGTACA	1 cut(s)	126
BstC8I	GCNNGC	5 cut(s)	31, 579, 1053, 1268, 1691
BstDEI	CTNAG	3 cut(s)	185, 696, 1685
BstDSI	CCRYGG	2 cut(s)	456, 1599
BstF5I	GGATG	3 cut(s)	760, 831, 1574
BstFNI	CGCG	1 cut(s)	480
BstHHI	GCGC	1 cut(s)	1938
BstMAI	GTCTC	3 cut(s)	159, 933, 1111
BstMWI	GCNNNNNNNGC	7 cut(s)	1300, 1309, 1466, 1669, 1690, 1926, 1935
BstNI	CCWGG	4 cut(s)	101, 210, 1251, 1494
BstNSI	RCATGY	2 cut(s)	1364, 1693
BstSCI	CCNGG	5 cut(s)	99, 208, 448, 1249, 1492
BstSFI	CTRYAG	1 cut(s)	1826
BstUI	CGCG	1 cut(s)	480
BstV2I	GAAGAC	2 cut(s)	423, 1546
BstX2I	RGATCY	3 cut(s)	16, 874, 1504
BstYI	RGATCY	3 cut(s)	16, 874, 1504
BsuI	GTATCC	1 cut(s)	97
BsuRI	GGCC	5 cut(s)	208, 1031, 1055, 1249, 1404
BtgI	CCRYGG	2 cut(s)	456, 1599
BtsCI	GGATG	3 cut(s)	760, 831, 1574
BtsI	GCAGTG	1 cut(s)	129
BtsIMutI	CAGTG	6 cut(s)	48, 129, 694, 963, 1289, 1548
Cac8I	GCNNGC	5 cut(s)	31, 579, 1053, 1268, 1691
CaiI	CAGNNNCTG	1 cut(s)	1565
CciI	TCATGA	2 cut(s)	466, 1342
CfoI	GCGC	1 cut(s)	1938
Cfr13I	GGNCC	2 cut(s)	206, 700
CseI	GACGC	1 cut(s)	390
Csp6I	GTAC	3 cut(s)	127, 826, 1143
CspCI	CAANNNNNGTGG	2 cut(s)	1347, 1382
CviQI	GTAC	3 cut(s)	127, 826, 1143
DdeI	CTNAG	3 cut(s)	185, 696, 1685
EaeI	YGGCCR	1 cut(s)	1402
Eam1104I	CTCTTC	1 cut(s)	255
EarI	CTCTTC	1 cut(s)	255
Ecl136II	GAGCTC	1 cut(s)	1920
Eco24I	GRGCYC	2 cut(s)	1660, 1922
Eco47I	GGWCC	1 cut(s)	700
Eco53kI	GAGCTC	1 cut(s)	1920
EcoICRI	GAGCTC	1 cut(s)	1920
EcoO109I	RGGNCCY	1 cut(s)	700
EcoRII	CCWGG	4 cut(s)	99, 208, 1249, 1492
EcoT22I	ATGCAT	1 cut(s)	235
EcoT38I	GRGCYC	2 cut(s)	1660, 1922
FaqI	GGGAC	2 cut(s)	686, 896
FauI	CCCGC	1 cut(s)	1056
FbaI	TGATCA	1 cut(s)	387
FblI	GTMKAC	1 cut(s)	279
FokI	GGATG	3 cut(s)	767, 818, 1561
FriOI	GRGCYC	2 cut(s)	1660, 1922
FspBI	CTAG	4 cut(s)	578, 1002, 1886, 1923
GlaI	GCGC	1 cut(s)	1937
GsuI	CTGGAG	2 cut(s)	954, 1515
HaeIII	GGCC	5 cut(s)	208, 1031, 1055, 1249, 1404
HapII	CCGG	3 cut(s)	450, 653, 1651
HgaI	GACGC	1 cut(s)	390
HhaI	GCGC	1 cut(s)	1938
Hin1I	GRCGYC	1 cut(s)	401
Hin6I	GCGC	1 cut(s)	1936
HinP1I	GCGC	1 cut(s)	1936
HincII	GTYRAC	1 cut(s)	280
HindII	GTYRAC	1 cut(s)	280
HindIII	AAGCTT	1 cut(s)	1670
HinfI	GANTC	4 cut(s)	22, 152, 548, 722
HpaII	CCGG	3 cut(s)	450, 653, 1651
HphI	GGTGA	5 cut(s)	298, 353, 1205, 1405, 1585
Hpy166II	GTNNAC	4 cut(s)	127, 280, 1371, 1860
Hpy188III	TCNNGA	7 cut(s)	254, 467, 1187, 1343, 1651, 1739, 1943
Hpy8I	GTNNAC	4 cut(s)	127, 280, 1371, 1860
Hpy99I	CGWCG	1 cut(s)	406
HpyAV	CCTTC	5 cut(s)	538, 659, 1271, 1600, 1832
HpyCH4III	ACNGT	6 cut(s)	131, 460, 689, 1193, 1465, 1552
HpyCH4IV	ACGT	1 cut(s)	828
HpyF10VI	GCNNNNNNNGC	7 cut(s)	1300, 1309, 1466, 1669, 1690, 1926, 1935
HpyF3I	CTNAG	3 cut(s)	185, 696, 1685
HpySE526I	ACGT	1 cut(s)	828
Hsp92I	GRCGYC	1 cut(s)	401
HspAI	GCGC	1 cut(s)	1936
Kpn2I	TCCGGA	1 cut(s)	1650
Ksp22I	TGATCA	1 cut(s)	387
LmnI	GCTCC	8 cut(s)	458, 727, 840, 913, 1105, 1173, 1533, 1783
LweI	GCATC	6 cut(s)	852, 1117, 1488, 1571, 1583, 1774
MaeI	CTAG	4 cut(s)	578, 1002, 1886, 1923
MaeII	ACGT	1 cut(s)	828
MaeIII	GTNAC	3 cut(s)	46, 131, 1193
MboII	GAAGA	8 cut(s)	242, 301, 428, 820, 904, 1127, 1408, 1546
MfeI	CAATTG	1 cut(s)	1304
MflI	RGATCY	3 cut(s)	16, 874, 1504
MhlI	GDGCHC	2 cut(s)	1660, 1922
MlsI	TGGCCA	1 cut(s)	1404
MluCI	AATT	5 cut(s)	66, 111, 924, 1304, 1348
MluNI	TGGCCA	1 cut(s)	1404
MlyI	GAGTC	1 cut(s)	161
MmeI	TCCRAC	2 cut(s)	384, 731
Mox20I	TGGCCA	1 cut(s)	1404
Mph1103I	ATGCAT	1 cut(s)	235
MroI	TCCGGA	1 cut(s)	1650
MroXI	GAANNNNTTC	3 cut(s)	474, 1134, 1260
MscI	TGGCCA	1 cut(s)	1404
MseI	TTAA	7 cut(s)	77, 803, 870, 1038, 1335, 1415, 1969
MslI	CAYNNNNRTG	2 cut(s)	908, 1598
Msp20I	TGGCCA	1 cut(s)	1404
MspA1I	CMGCKG	1 cut(s)	574
MspCI	CTTAAG	1 cut(s)	802
MspI	CCGG	3 cut(s)	450, 653, 1651
MspR9I	CCNGG	5 cut(s)	101, 210, 450, 1251, 1494
MunI	CAATTG	1 cut(s)	1304
Mva1269I	GAATGC	1 cut(s)	235
MvaI	CCWGG	4 cut(s)	101, 210, 1251, 1494
MvnI	CGCG	1 cut(s)	480
MwoI	GCNNNNNNNGC	7 cut(s)	1300, 1309, 1466, 1669, 1690, 1926, 1935
NciI	CCSGG	1 cut(s)	450
NheI	GCTAGC	1 cut(s)	577
NlaIV	GGNNCC	6 cut(s)	454, 702, 860, 1169, 1311, 1506
NmuCI	GTSAC	2 cut(s)	46, 1193
NsiI	ATGCAT	1 cut(s)	235
NspI	RCATGY	2 cut(s)	1364, 1693
OliI	CACNNNNGTG	1 cut(s)	1598
PaeI	GCATGC	1 cut(s)	1693
PagI	TCATGA	2 cut(s)	466, 1342
PctI	GAATGC	1 cut(s)	235
PdmI	GAANNNNTTC	3 cut(s)	474, 1134, 1260
PfeI	GAWTC	3 cut(s)	22, 548, 722
PfoI	TCCNGGA	1 cut(s)	99
PleI	GAGTC	1 cut(s)	160
PpsI	GAGTC	1 cut(s)	160
PpuMI	RGGWCCY	1 cut(s)	700
Psp124BI	GAGCTC	1 cut(s)	1922
Psp5II	RGGWCCY	1 cut(s)	700
Psp6I	CCWGG	4 cut(s)	99, 208, 1249, 1492
PspGI	CCWGG	4 cut(s)	99, 208, 1249, 1492
PspN4I	GGNNCC	6 cut(s)	454, 702, 860, 1169, 1311, 1506
PspPI	GGNCC	2 cut(s)	206, 700
PspPPI	RGGWCCY	1 cut(s)	700
PstI	CTGCAG	1 cut(s)	1830
PstNI	CAGNNNCTG	1 cut(s)	1565
PsuI	RGATCY	3 cut(s)	16, 874, 1504
PvuII	CAGCTG	1 cut(s)	574
RsaI	GTAC	3 cut(s)	128, 827, 1144
RsaNI	GTAC	3 cut(s)	127, 826, 1143
RseI	CAYNNNNRTG	2 cut(s)	908, 1598
SacI	GAGCTC	1 cut(s)	1922
SalI	GTCGAC	1 cut(s)	278
SaqAI	TTAA	7 cut(s)	77, 803, 870, 1038, 1335, 1415, 1969
Sau96I	GGNCC	2 cut(s)	206, 700
SchI	GAGTC	1 cut(s)	161
ScrFI	CCNGG	5 cut(s)	101, 210, 450, 1251, 1494
SduI	GDGCHC	2 cut(s)	1660, 1922
SfaNI	GCATC	6 cut(s)	852, 1117, 1488, 1571, 1583, 1774
SfcI	CTRYAG	1 cut(s)	1826
SinI	GGWCC	1 cut(s)	700
SmiMI	CAYNNNNRTG	2 cut(s)	908, 1598
SmlI	CTYRAG	3 cut(s)	161, 802, 1237
SmoI	CTYRAG	3 cut(s)	161, 802, 1237
SphI	GCATGC	1 cut(s)	1693
Sse9I	AATT	5 cut(s)	66, 111, 924, 1304, 1348
SsiI	CCGC	4 cut(s)	108, 363, 480, 1049
SspI	AATATT	1 cut(s)	1849
SspMI	CTAG	4 cut(s)	578, 1002, 1886, 1923
SstI	GAGCTC	1 cut(s)	1922
StyD4I	CCNGG	5 cut(s)	99, 208, 448, 1249, 1492
TaaI	ACNGT	6 cut(s)	131, 460, 689, 1193, 1465, 1552
TaiI	ACGT	1 cut(s)	831
TaqI	TCGA	5 cut(s)	266, 279, 784, 1059, 1628
TasI	AATT	5 cut(s)	66, 111, 924, 1304, 1348
TatI	WGTACW	1 cut(s)	126
TauI	GCSGC	2 cut(s)	365, 483
TfiI	GAWTC	3 cut(s)	22, 548, 722
Tru1I	TTAA	7 cut(s)	77, 803, 870, 1038, 1335, 1415, 1969
Tru9I	TTAA	7 cut(s)	77, 803, 870, 1038, 1335, 1415, 1969
TscAI	CASTG	6 cut(s)	55, 129, 694, 970, 1289, 1555
TseFI	GTSAC	2 cut(s)	46, 1193
Tsp45I	GTSAC	2 cut(s)	46, 1193
TspDTI	ATGAA	8 cut(s)	104, 455, 483, 1080, 1082, 1146, 1649, 1950
TspGWI	ACGGA	2 cut(s)	1130, 1588
TspRI	CASTG	6 cut(s)	55, 129, 694, 970, 1289, 1555
Vha464I	CTTAAG	1 cut(s)	802
VpaK11BI	GGWCC	1 cut(s)	700
XapI	RAATTY	1 cut(s)	924
XceI	RCATGY	2 cut(s)	1364, 1693
XmiI	GTMKAC	1 cut(s)	279
XmnI	GAANNNNTTC	3 cut(s)	474, 1134, 1260
XspI	CTAG	4 cut(s)	578, 1002, 1886, 1923
Zsp2I	ATGCAT	1 cut(s)	235

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.