RchiOBHm_Chr5g0001861

protein ubiquitination

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0008844
Physical Location & Seq
Forward (+)
1031954 .. 1032973
1020 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ28325

Sequence Viewer

Length: 711 bp
ATGCCCAGTTATAAAAGTGTCGCCCGTATTTACAATTTCTTCTTGGAAAAGAAATGGAGTGAGATAAGGATCTTTTACGAGGAAAACAATTTTGATGTGTTCCTGCTTCTTAATGCTTTGGGAGATACGGCTCTTCACCTTGCAGTGTACAGTGAAGACCTGGAATTGGTGAGATCTCTGCTGAAGATAGTGTACGAAGACAATCCTTTTGCCGAAGCTGGTATAAAATTTCCCTCATCAGCGAATGCATCTGGAAACACACCACTTCATGAGGCAGCTTCAATAGGAAATGTTAAATTGGTAAAGCTGATATTGAGTTGTGGTGGCGATCATATGCTGACGACAGTGAACAAGAATGGTGAAACACCTTTTTTTATGGCCGCACTTTATGGCCAGACCAATTTGATAAAAAATTTGGTGAACGATCTTAAGGACATAGATTATCATATCCTGTCTAATACCTCCACCATGCTTCATGAGGCTATCCGCGGCTATTACTTTGAAACAGCTCTGGAATTGTTAGACCCAAAATACCGCATACCGAAGGATTGGGTGGATTCAGATGGCATGACCTGCTTTCAGCTGCTAGCTGACATTCCCTCTGCTTTCAAGAGTGGATATTCCTTTGACATATTGGAGAAACTGATCTATGACGGTCTGTCAGAGACCGCAATCCAATTTGCTTACCATTTCACTTTTAAGGAGCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 PGG

Protein Analysis

236

Amino Acids

26.77

Weight (kDa)

4.95

Isoelectric Point (pI)

23.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 36 - 111 7.4e-10 Ankyrin repeats (3 copies)
Ank PF00023 85 - 110 8.5e-06 Ankyrin repeat
Ank_2 PF12796 96 - 177 2.4e-08 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 12
Acc36I ACCTGC 1 cut(s) 581
AccII CGCG 1 cut(s) 489
AciI CCGC 5 cut(s) 381, 487, 489, 535, 669
AclWI GGATC 1 cut(s) 77
AcoI YGGCCR 2 cut(s) 378, 391
AcsI RAATTY 2 cut(s) 227, 412
AcuI CTGAAG 1 cut(s) 203
AfaI GTAC 2 cut(s) 149, 194
AfiI CCNNNNNNNGG 1 cut(s) 166
AflII CTTAAG 1 cut(s) 428
AgsI TTSAA 3 cut(s) 282, 503, 610
AhdI GACNNNNNGTC 1 cut(s) 658
AjnI CCWGG 1 cut(s) 159
AluBI AGCT 7 cut(s) 218, 278, 307, 509, 583, 590, 706
AluI AGCT 7 cut(s) 218, 278, 307, 509, 583, 590, 706
Alw26I GTCTC 1 cut(s) 659
AlwI GGATC 1 cut(s) 77
AoxI GGCC 2 cut(s) 378, 391
ApeKI GCWGC 2 cut(s) 275, 583
ApoI RAATTY 2 cut(s) 227, 412
ArsI GACNNNNNNTTYG 2 cut(s) 191, 223
AsuHPI GGTGA 4 cut(s) 128, 181, 371, 430
AsuNHI GCTAGC 1 cut(s) 586
BalI TGGCCA 1 cut(s) 393
BarI GAAGNNNNNNTAC 2 cut(s) 176, 208
BbsI GAAGAC 2 cut(s) 162, 204
BbvI GCAGC 2 cut(s) 287, 570
BccI CCATC 1 cut(s) 557
BceAI ACGGC 1 cut(s) 144
BciT130I CCWGG 1 cut(s) 161
BcoDI GTCTC 1 cut(s) 659
BfaI CTAG 1 cut(s) 587
BfrI CTTAAG 1 cut(s) 428
BfuAI ACCTGC 1 cut(s) 581
BglII AGATCT 1 cut(s) 173
BisI GCNGC 4 cut(s) 276, 381, 490, 584
BlsI GCNGC 4 cut(s) 277, 382, 491, 585
Bme1390I CCNGG 1 cut(s) 161
BmeRI GACNNNNNGTC 1 cut(s) 658
BmrFI CCNGG 1 cut(s) 161
BmsI GCATC 1 cut(s) 257
BmtI GCTAGC 1 cut(s) 590
BpiI GAAGAC 2 cut(s) 162, 204
BsaBI GATNNNNATC 1 cut(s) 68
BsaI GGTCTC 1 cut(s) 659
BsaJI CCNNGG 1 cut(s) 487
Bsc4I CCNNNNNNNGG 1 cut(s) 166
Bse1I ACTGG 1 cut(s) 6
Bse8I GATNNNNATC 1 cut(s) 68
BseBI CCWGG 1 cut(s) 161
BseDI CCNNGG 1 cut(s) 487
BseJI GATNNNNATC 1 cut(s) 68
BseLI CCNNNNNNNGG 1 cut(s) 166
BseNI ACTGG 1 cut(s) 6
BseXI GCAGC 2 cut(s) 287, 570
Bsh1236I CGCG 1 cut(s) 489
BshFI GGCC 2 cut(s) 380, 393
BslI CCNNNNNNNGG 1 cut(s) 166
BsmAI GTCTC 1 cut(s) 659
BsmI GAATGC 1 cut(s) 250
BsnI GGCC 2 cut(s) 380, 393
Bso31I GGTCTC 1 cut(s) 659
Bsp1407I TGTACA 1 cut(s) 147
Bsp143I GATC 5 cut(s) 69, 173, 328, 424, 645
BspACI CCGC 5 cut(s) 381, 487, 489, 535, 669
BspANI GGCC 2 cut(s) 380, 393
BspFNI CGCG 1 cut(s) 489
BspHI TCATGA 2 cut(s) 268, 475
BspMI ACCTGC 1 cut(s) 581
BspOI GCTAGC 1 cut(s) 590
BspPI GGATC 1 cut(s) 77
BspQI GCTCTTC 1 cut(s) 138
BspTI CTTAAG 1 cut(s) 428
BspTNI GGTCTC 1 cut(s) 659
BsrGI TGTACA 1 cut(s) 147
BsrI ACTGG 1 cut(s) 6
BssECI CCNNGG 1 cut(s) 487
BssMI GATC 5 cut(s) 69, 173, 328, 424, 645
Bst2UI CCWGG 1 cut(s) 161
Bst4CI ACNGT 3 cut(s) 152, 346, 656
Bst6I CTCTTC 1 cut(s) 138
BstAFI CTTAAG 1 cut(s) 428
BstAPI GCANNNNNTGC 1 cut(s) 573
BstAUI TGTACA 1 cut(s) 147
BstC8I GCNNGC 1 cut(s) 588
BstDSI CCRYGG 1 cut(s) 487
BstFNI CGCG 1 cut(s) 489
BstKTI GATC 5 cut(s) 72, 176, 331, 427, 648
BstMAI GTCTC 1 cut(s) 659
BstMBI GATC 5 cut(s) 69, 173, 328, 424, 645
BstMWI GCNNNNNNNGC 1 cut(s) 573
BstNI CCWGG 1 cut(s) 161
BstSCI CCNGG 1 cut(s) 159
BstUI CGCG 1 cut(s) 489
BstV1I GCAGC 2 cut(s) 287, 570
BstV2I GAAGAC 2 cut(s) 162, 204
BstX2I RGATCY 2 cut(s) 69, 173
BstYI RGATCY 2 cut(s) 69, 173
BsuRI GGCC 2 cut(s) 380, 393
BtgI CCRYGG 1 cut(s) 487
BtsI GCAGTG 1 cut(s) 150
BtsIMutI CAGTG 3 cut(s) 150, 157, 351
BveI ACCTGC 1 cut(s) 581
Cac8I GCNNGC 1 cut(s) 588
CciI TCATGA 2 cut(s) 268, 475
Cfr42I CCGCGG 1 cut(s) 490
Csp6I GTAC 2 cut(s) 148, 193
CviAII CATG 4 cut(s) 269, 469, 476, 568
CviQI GTAC 2 cut(s) 148, 193
DpnI GATC 5 cut(s) 71, 175, 330, 426, 647
DpnII GATC 5 cut(s) 69, 173, 328, 424, 645
DriI GACNNNNNGTC 1 cut(s) 658
EaeI YGGCCR 2 cut(s) 378, 391
Eam1104I CTCTTC 1 cut(s) 138
Eam1105I GACNNNNNGTC 1 cut(s) 658
EarI CTCTTC 1 cut(s) 138
Eco31I GGTCTC 1 cut(s) 659
Eco57I CTGAAG 1 cut(s) 203
EcoRII CCWGG 1 cut(s) 159
EcoT22I ATGCAT 1 cut(s) 250
FaeI CATG 4 cut(s) 272, 472, 479, 571
FatI CATG 4 cut(s) 268, 468, 475, 567
FauNDI CATATG 1 cut(s) 333
Fnu4HI GCNGC 4 cut(s) 276, 381, 490, 584
Fsp4HI GCNGC 4 cut(s) 276, 381, 490, 584
FspBI CTAG 1 cut(s) 587
GluI GCNGC 4 cut(s) 276, 381, 490, 584
HaeIII GGCC 2 cut(s) 380, 393
Hin1II CATG 4 cut(s) 272, 472, 479, 571
HinfI GANTC 1 cut(s) 557
HphI GGTGA 4 cut(s) 128, 181, 371, 430
Hpy166II GTNNAC 4 cut(s) 148, 193, 349, 421
Hpy188I TCNGA 2 cut(s) 562, 664
Hpy188III TCNNGA 5 cut(s) 252, 269, 476, 512, 610
Hpy8I GTNNAC 4 cut(s) 148, 193, 349, 421
HpyAV CCTTC 1 cut(s) 538
HpyCH4III ACNGT 3 cut(s) 152, 346, 656
HpyCH4V TGCA 2 cut(s) 143, 248
HpyF10VI GCNNNNNNNGC 1 cut(s) 573
Hsp92II CATG 4 cut(s) 272, 472, 479, 571
KspI CCGCGG 1 cut(s) 490
Kzo9I GATC 5 cut(s) 69, 173, 328, 424, 645
LguI GCTCTTC 1 cut(s) 138
LmnI GCTCC 1 cut(s) 703
Lsp1109I GCAGC 2 cut(s) 287, 570
LweI GCATC 1 cut(s) 257
MaeI CTAG 1 cut(s) 587
MalI GATC 5 cut(s) 71, 175, 330, 426, 647
MboI GATC 5 cut(s) 69, 173, 328, 424, 645
MboII GAAGA 5 cut(s) 31, 125, 167, 196, 209
MflI RGATCY 2 cut(s) 69, 173
MlsI TGGCCA 1 cut(s) 393
MluCI AATT 9 cut(s) 34, 88, 164, 227, 296, 400, 412, 515, 677
MluNI TGGCCA 1 cut(s) 393
MnlI CCTC 6 cut(s) 73, 244, 265, 472, 472, 610
Mox20I TGGCCA 1 cut(s) 393
Mph1103I ATGCAT 1 cut(s) 250
MscI TGGCCA 1 cut(s) 393
MseI TTAA 4 cut(s) 111, 294, 429, 699
Msp20I TGGCCA 1 cut(s) 393
MspA1I CMGCKG 2 cut(s) 489, 583
MspCI CTTAAG 1 cut(s) 428
MspR9I CCNGG 1 cut(s) 161
Mva1269I GAATGC 1 cut(s) 250
MvaI CCWGG 1 cut(s) 161
MvnI CGCG 1 cut(s) 489
MwoI GCNNNNNNNGC 1 cut(s) 573
NdeI CATATG 1 cut(s) 333
NdeII GATC 5 cut(s) 69, 173, 328, 424, 645
NheI GCTAGC 1 cut(s) 586
NlaIII CATG 4 cut(s) 272, 472, 479, 571
NsiI ATGCAT 1 cut(s) 250
PagI TCATGA 2 cut(s) 268, 475
PciSI GCTCTTC 1 cut(s) 138
PctI GAATGC 1 cut(s) 250
PfeI GAWTC 1 cut(s) 557
PkrI GCNGC 4 cut(s) 277, 382, 491, 585
PsiI TTATAA 1 cut(s) 12
Psp6I CCWGG 1 cut(s) 159
PspGI CCWGG 1 cut(s) 159
PsuI RGATCY 2 cut(s) 69, 173
PvuII CAGCTG 1 cut(s) 583
RsaI GTAC 2 cut(s) 149, 194
RsaNI GTAC 2 cut(s) 148, 193
SacII CCGCGG 1 cut(s) 490
SapI GCTCTTC 1 cut(s) 138
SaqAI TTAA 4 cut(s) 111, 294, 429, 699
SatI GCNGC 4 cut(s) 276, 381, 490, 584
Sau3AI GATC 5 cut(s) 69, 173, 328, 424, 645
ScrFI CCNGG 1 cut(s) 161
SfaNI GCATC 1 cut(s) 257
Sfr303I CCGCGG 1 cut(s) 490
SgrBI CCGCGG 1 cut(s) 490
SmlI CTYRAG 1 cut(s) 428
SmoI CTYRAG 1 cut(s) 428
Sse9I AATT 9 cut(s) 34, 88, 164, 227, 296, 400, 412, 515, 677
SsiI CCGC 5 cut(s) 381, 487, 489, 535, 669
SspMI CTAG 1 cut(s) 587
StyD4I CCNGG 1 cut(s) 159
TaaI ACNGT 3 cut(s) 152, 346, 656
TasI AATT 9 cut(s) 34, 88, 164, 227, 296, 400, 412, 515, 677
TatI WGTACW 1 cut(s) 147
TauI GCSGC 2 cut(s) 383, 492
TfiI GAWTC 1 cut(s) 557
Tru1I TTAA 4 cut(s) 111, 294, 429, 699
Tru9I TTAA 4 cut(s) 111, 294, 429, 699
TscAI CASTG 3 cut(s) 150, 157, 351
TseI GCWGC 2 cut(s) 275, 583
TspDTI ATGAA 2 cut(s) 257, 464
TspRI CASTG 3 cut(s) 150, 157, 351
Vha464I CTTAAG 1 cut(s) 428
XapI RAATTY 2 cut(s) 227, 412
XspI CTAG 1 cut(s) 587
Zsp2I ATGCAT 1 cut(s) 250
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.