Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb3

Orthogroup ID

OG0001460

Physical Location & Seq

Genomic Coordinates Forward (+)

2759120 .. 2761238

Gene Structure 2119 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_3g04784.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 942 bp

ATGGACCAAATCCAGCACAAGTATATAACCGTACAAGGCCTGAAGCTCCACGTGGCCGACGTCGGAACAGGTCCAAATGTGGTCGTGTTCCTACATGGATTCCCGGAAATATGGTACACGTGGCGCCACCAGATGATCGCTCTGGCCAACGCCGGATTTCGGGCAATCGCTCCCGACTACCGCGGGTATGGACTCTCTGACCCGCCTCCACAACCCGAGAAGGCCTCCTTCCTTGACCTTGACAATGACCTCCTTGCAATTCTTGATGCCCTTGCCATTCCTAAGGTTTTCCTCATCGGAAAAGATTTTGGAGCTTGGCCGGCGTACATATTTGCCGGTCTGCACCCAGAAAGGACCTTGGGAGTTGTAACAATGGGAGTGCCATACATGCCAAAGTCAAGTCGTCCCCAATTCAATAACCACCTTCCTGAAGGTTTCTATATTTCAAGATGGCAGGAACCAGGTGGCAGGGCTGAAGCTGATTTTGGACGCCTTGATGCGAAAACAGTTATTCGCAATGTGTACATTCTTTTCTCGAGGAGTGAAATACCAATAGCTTCAGAAAACCAGGAGATCATGGACTTGGTGGACTCGTCCACGCCTCTCCCCCCTTGGTTCACTGAGGAAGATCTTGAGGTTTATGGAAAGTTGTACGAGAAATCTGGTTTTCAAACTGCATTGCAACTTCCATATAGGGCAATGAAACATGAGTTGGGCATAGCTGATTCAACCATTGAAGCTCCAGCACTTTTCATAATGGGAACAAAGGATTATGTATACGAATTTCCAGGGATGAAAGATTACATAACCAGTGCAAAGCTGAAAGAGTTTGTCCCGGATTTGGAGATTGTATTTCTGCCAGAAGGAACTCATTTTGTTCATGAACAACTACCTGACCAGGTTAATGAGCTCATCCTCACCTTTCTCCGAAAGCACATCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000287 GO:0001676 GO:0002532 GO:0002538 GO:0002539 GO:0003008 GO:0003013 GO:0003674 GO:0003824 GO:0004301 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005829 GO:0006082 GO:0006629 GO:0006631 GO:0006793 GO:0006796 GO:0006950 GO:0006952 GO:0006954 GO:0007600 GO:0008015 GO:0008150 GO:0008152 GO:0008217 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0016801 GO:0016803 GO:0019233 GO:0019752 GO:0030258 GO:0032501 GO:0032787 GO:0033559 GO:0042577 GO:0042578 GO:0042579 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043651 GO:0044237 GO:0044238 GO:0044255 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0045777 GO:0046839 GO:0046872 GO:0050877 GO:0050896 GO:0065007 GO:0065008 GO:0071704

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Abhydrolase_1 Abhydrolase_6

Protein Analysis

314

Amino Acids

35.46

Weight (kDa)

5.2

Isoelectric Point (pI)

40.75

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Abhydrolase_1	PF00561	26 - 293	6.6e-25	alpha/beta hydrolase fold
Hydrolase_4	PF12146	27 - 126	1.7e-07	Serine aminopeptidase, S33
Abhydrolase_6	PF12697	28 - 303	2.4e-15	Alpha/beta hydrolase family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001460)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g05700 FvH4_3g04781 FvH4_3g04781 FvH4_3g04781 FvH4_3g04784 FvH4_3g04784 FvH4_3g04790 FvH4_3g04790 FvH4_3g04790
malus_domestica	MD05G1316900.v1.1 MD05G1317400.v1.1 MD10G1297200.v1.1
prunus_persica	Prupe.4G043200_v2.0.a1 Prupe.4G043200_v2.0.a1
pyrus_communis	pycom05g29400 pycom10g25010 pycom10g25060
rosa_chinensis	RchiOBHm_Chr5g0007821 RchiOBHm_Chr5g0007841 RchiOBHm_Chr5g0007871 RchiOBHm_Chr5g0007881
rosa_laevigata	RLG00000031499 RLG00000031500 RLG00000031501
rosa_multiflora	Rmu_co8472583.1_g000001 Rmu_sc0005098.1_g000007 Rmu_sc0005098.1_g000012
rosa_roxburghii	Rroxscaffold_1G00068550 Rroxscaffold_1G00068560 Rroxscaffold_1G00068580
rosa_rugosa	Rorug04G0430600 Rorug04G0430600 Rorug04G0430600 Rorug04G0430600 Rorug04G0430700 Rorug04G0430800
rosa_samantha	Rh5AG059200 Rh5AG059300 Rh5AG059600 Rh5AG059700 Rh5BG056200 Rh5BG056300 Rh5BG056400 Rh5BG056500 Rh5CG066000 Rh5CG066100 Rh5CG066400 Rh5DG055300 Rh5DG055500
rosa_wichuraiana	Rw5G006050 Rw5G006060 Rw5G006070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	63
AccB1I	GGYRCC	1 cut(s)	123
AccI	GTMKAC	1 cut(s)	777
AccII	CGCG	1 cut(s)	183
AciI	CCGC	3 cut(s)	181, 183, 203
AcoI	YGGCCR	3 cut(s)	54, 144, 317
AcsI	RAATTY	1 cut(s)	782
AcuI	CTGAAG	4 cut(s)	62, 450, 495, 543
AcvI	CACGTG	2 cut(s)	52, 120
AcyI	GRCGYC	3 cut(s)	60, 124, 490
AfaI	GTAC	5 cut(s)	33, 116, 326, 524, 653
AfiI	CCNNNNNNNGG	2 cut(s)	159, 841
AflIII	ACRYGT	1 cut(s)	117
AgsI	TTSAA	5 cut(s)	415, 447, 671, 729, 737
AjnI	CCWGG	4 cut(s)	460, 567, 787, 897
AjuI	GAANNNNNNNTTGG	4 cut(s)	468, 500, 695, 727
AluBI	AGCT	8 cut(s)	46, 314, 479, 557, 722, 740, 820, 910
AluI	AGCT	8 cut(s)	46, 314, 479, 557, 722, 740, 820, 910
Alw21I	GWGCWC	1 cut(s)	912
Ama87I	CYCGRG	2 cut(s)	215, 535
AoxI	GGCC	5 cut(s)	37, 54, 144, 222, 317
ApoI	RAATTY	1 cut(s)	782
AspLEI	GCGC	1 cut(s)	126
AspS9I	GGNCC	3 cut(s)	4, 71, 354
AsuC2I	CCSGG	2 cut(s)	104, 836
AsuHPI	GGTGA	1 cut(s)	910
AvaI	CYCGRG	2 cut(s)	215, 535
AvaII	GGWCC	3 cut(s)	4, 71, 354
AxyI	CCTNAGG	1 cut(s)	282
BalI	TGGCCA	1 cut(s)	146
BanI	GGYRCC	1 cut(s)	123
BanII	GRGCYC	1 cut(s)	912
BbrPI	CACGTG	2 cut(s)	52, 120
Bbv12I	GWGCWC	1 cut(s)	912
BccI	CCATC	1 cut(s)	444
BciT130I	CCWGG	4 cut(s)	462, 569, 789, 899
BcnI	CCSGG	2 cut(s)	104, 836
BfoI	RGCGCY	1 cut(s)	127
BglII	AGATCT	1 cut(s)	628
Bme1390I	CCNGG	6 cut(s)	104, 462, 569, 789, 836, 899
Bme18I	GGWCC	3 cut(s)	4, 71, 354
BmeT110I	CYCGRG	2 cut(s)	215, 535
BmgT120I	GGNCC	3 cut(s)	4, 71, 354
BmiI	GGNNCC	2 cut(s)	125, 459
BmrFI	CCNGG	6 cut(s)	104, 462, 569, 789, 836, 899
BmsI	GCATC	2 cut(s)	256, 487
BplI	GAGNNNNNCTC	2 cut(s)	209, 241
BpmI	CTGGAG	1 cut(s)	726
BpuEI	CTTGAG	1 cut(s)	653
BpuMI	CCSGG	2 cut(s)	104, 836
BsaAI	YACGTR	2 cut(s)	52, 120
BsaHI	GRCGYC	3 cut(s)	60, 124, 490
BsaJI	CCNNGG	4 cut(s)	181, 357, 611, 788
Bsc4I	CCNNNNNNNGG	2 cut(s)	159, 841
Bse118I	RCCGGY	2 cut(s)	319, 335
Bse1I	ACTGG	1 cut(s)	810
Bse21I	CCTNAGG	1 cut(s)	282
Bse3DI	GCAATG	3 cut(s)	523, 677, 705
BseBI	CCWGG	4 cut(s)	462, 569, 789, 899
BseDI	CCNNGG	4 cut(s)	181, 357, 611, 788
BseGI	GGATG	2 cut(s)	798, 912
BseLI	CCNNNNNNNGG	2 cut(s)	159, 841
BseMI	GCAATG	3 cut(s)	523, 677, 705
BseMII	CTCAG	1 cut(s)	612
BseNI	ACTGG	1 cut(s)	810
BseRI	GAGGAG	1 cut(s)	553
BsgI	GTGCAG	1 cut(s)	326
Bsh1236I	CGCG	1 cut(s)	183
BshFI	GGCC	5 cut(s)	39, 56, 146, 224, 319
BshNI	GGYRCC	1 cut(s)	123
BsiHKAI	GWGCWC	1 cut(s)	912
BsiHKCI	CYCGRG	2 cut(s)	215, 535
BsiSI	CCGG	5 cut(s)	104, 153, 320, 336, 836
BslFI	GGGAC	2 cut(s)	390, 818
BslI	CCNNNNNNNGG	2 cut(s)	159, 841
BsmFI	GGGAC	2 cut(s)	390, 818
BsnI	GGCC	5 cut(s)	39, 56, 146, 224, 319
BsoBI	CYCGRG	2 cut(s)	215, 535
Bsp1286I	GDGCHC	1 cut(s)	912
Bsp1407I	TGTACA	1 cut(s)	522
Bsp143I	GATC	3 cut(s)	135, 573, 628
BspACI	CCGC	3 cut(s)	181, 183, 203
BspANI	GGCC	5 cut(s)	39, 56, 146, 224, 319
BspCNI	CTCAG	1 cut(s)	613
BspFNI	CGCG	1 cut(s)	183
BspHI	TCATGA	1 cut(s)	880
BspLI	GGNNCC	2 cut(s)	125, 459
BspT107I	GGYRCC	1 cut(s)	123
BsrDI	GCAATG	3 cut(s)	523, 677, 705
BsrFI	RCCGGY	2 cut(s)	319, 335
BsrGI	TGTACA	1 cut(s)	522
BsrI	ACTGG	1 cut(s)	810
BssAI	RCCGGY	2 cut(s)	319, 335
BssECI	CCNNGG	4 cut(s)	181, 357, 611, 788
BssMI	GATC	3 cut(s)	135, 573, 628
BssNAI	GTATAC	1 cut(s)	778
BssNI	GRCGYC	3 cut(s)	60, 124, 490
BssT1I	CCWWGG	2 cut(s)	357, 611
Bst1107I	GTATAC	1 cut(s)	778
Bst2UI	CCWGG	4 cut(s)	462, 569, 789, 899
Bst4CI	ACNGT	2 cut(s)	31, 508
BstACI	GRCGYC	3 cut(s)	60, 124, 490
BstAUI	TGTACA	1 cut(s)	522
BstBAI	YACGTR	2 cut(s)	52, 120
BstC8I	GCNNGC	1 cut(s)	321
BstDEI	CTNAG	2 cut(s)	282, 621
BstDSI	CCRYGG	1 cut(s)	181
BstF5I	GGATG	2 cut(s)	798, 912
BstFNI	CGCG	1 cut(s)	183
BstH2I	RGCGCY	1 cut(s)	127
BstHHI	GCGC	1 cut(s)	126
BstKTI	GATC	3 cut(s)	138, 576, 631
BstMBI	GATC	3 cut(s)	135, 573, 628
BstMWI	GCNNNNNNNGC	2 cut(s)	320, 388
BstNI	CCWGG	4 cut(s)	462, 569, 789, 899
BstNSI	RCATGY	1 cut(s)	391
BstSCI	CCNGG	6 cut(s)	102, 460, 567, 787, 834, 897
BstUI	CGCG	1 cut(s)	183
BstX2I	RGATCY	1 cut(s)	628
BstYI	RGATCY	1 cut(s)	628
BstZ17I	GTATAC	1 cut(s)	778
Bsu36I	CCTNAGG	1 cut(s)	282
BsuRI	GGCC	5 cut(s)	39, 56, 146, 224, 319
BtgI	CCRYGG	1 cut(s)	181
BtsCI	GGATG	2 cut(s)	798, 912
BtsIMutI	CAGTG	2 cut(s)	618, 817
Cac8I	GCNNGC	1 cut(s)	321
CciI	TCATGA	1 cut(s)	880
CfoI	GCGC	1 cut(s)	126
Cfr10I	RCCGGY	2 cut(s)	319, 335
Cfr13I	GGNCC	3 cut(s)	4, 71, 354
Cfr42I	CCGCGG	1 cut(s)	184
CseI	GACGC	1 cut(s)	498
CsiI	ACCWGGT	2 cut(s)	460, 897
Csp6I	GTAC	5 cut(s)	32, 115, 325, 523, 652
CviAII	CATG	5 cut(s)	95, 388, 577, 707, 881
CviQI	GTAC	5 cut(s)	32, 115, 325, 523, 652
DdeI	CTNAG	2 cut(s)	282, 621
DinI	GGCGCC	1 cut(s)	125
DpnI	GATC	3 cut(s)	137, 575, 630
DpnII	GATC	3 cut(s)	135, 573, 628
EaeI	YGGCCR	3 cut(s)	54, 144, 317
Ecl136II	GAGCTC	1 cut(s)	910
Eco130I	CCWWGG	2 cut(s)	357, 611
Eco147I	AGGCCT	2 cut(s)	39, 224
Eco24I	GRGCYC	1 cut(s)	912
Eco47I	GGWCC	3 cut(s)	4, 71, 354
Eco53kI	GAGCTC	1 cut(s)	910
Eco57I	CTGAAG	4 cut(s)	62, 450, 495, 543
Eco72I	CACGTG	2 cut(s)	52, 120
Eco81I	CCTNAGG	1 cut(s)	282
Eco88I	CYCGRG	2 cut(s)	215, 535
EcoICRI	GAGCTC	1 cut(s)	910
EcoO109I	RGGNCCY	1 cut(s)	354
EcoRII	CCWGG	4 cut(s)	460, 567, 787, 897
EcoT14I	CCWWGG	2 cut(s)	357, 611
EcoT38I	GRGCYC	1 cut(s)	912
EgeI	GGCGCC	1 cut(s)	125
EheI	GGCGCC	1 cut(s)	125
ErhI	CCWWGG	2 cut(s)	357, 611
FaeI	CATG	5 cut(s)	98, 391, 580, 710, 884
FalI	AAGNNNNNCTT	2 cut(s)	212, 244
FaqI	GGGAC	2 cut(s)	390, 818
FatI	CATG	5 cut(s)	94, 387, 576, 706, 880
FauI	CCCGC	2 cut(s)	176, 210
FblI	GTMKAC	1 cut(s)	777
FokI	GGATG	2 cut(s)	805, 899
FriOI	GRGCYC	1 cut(s)	912
GlaI	GCGC	1 cut(s)	125
GsuI	CTGGAG	1 cut(s)	726
HaeII	RGCGCY	1 cut(s)	127
HaeIII	GGCC	5 cut(s)	39, 56, 146, 224, 319
HapII	CCGG	5 cut(s)	104, 153, 320, 336, 836
HgaI	GACGC	1 cut(s)	498
HhaI	GCGC	1 cut(s)	126
Hin1I	GRCGYC	3 cut(s)	60, 124, 490
Hin1II	CATG	5 cut(s)	98, 391, 580, 710, 884
Hin6I	GCGC	1 cut(s)	124
HinP1I	GCGC	1 cut(s)	124
HinfI	GANTC	4 cut(s)	99, 192, 590, 725
HpaII	CCGG	5 cut(s)	104, 153, 320, 336, 836
HphI	GGTGA	1 cut(s)	910
Hpy166II	GTNNAC	6 cut(s)	117, 523, 589, 597, 618, 778
Hpy188I	TCNGA	6 cut(s)	65, 199, 299, 562, 929, 941
Hpy188III	TCNNGA	7 cut(s)	173, 263, 428, 447, 535, 632, 881
Hpy8I	GTNNAC	6 cut(s)	117, 523, 589, 597, 618, 778
Hpy99I	CGWCG	2 cut(s)	62, 65
HpyAV	CCTTC	5 cut(s)	214, 238, 425, 434, 857
HpyCH4III	ACNGT	2 cut(s)	31, 508
HpyCH4IV	ACGT	3 cut(s)	51, 60, 119
HpyCH4V	TGCA	5 cut(s)	257, 343, 677, 682, 815
HpyF10VI	GCNNNNNNNGC	2 cut(s)	320, 388
HpyF3I	CTNAG	2 cut(s)	282, 621
HpySE526I	ACGT	3 cut(s)	51, 60, 119
Hsp92I	GRCGYC	3 cut(s)	60, 124, 490
Hsp92II	CATG	5 cut(s)	98, 391, 580, 710, 884
HspAI	GCGC	1 cut(s)	124
KasI	GGCGCC	1 cut(s)	123
KroI	GCCGGC	1 cut(s)	319
KroNI	GCCGGC	1 cut(s)	321
KspI	CCGCGG	1 cut(s)	184
Kzo9I	GATC	3 cut(s)	135, 573, 628
LmnI	GCTCC	4 cut(s)	51, 175, 311, 745
LweI	GCATC	2 cut(s)	256, 487
MabI	ACCWGGT	2 cut(s)	460, 897
MaeII	ACGT	3 cut(s)	51, 60, 119
MaeIII	GTNAC	1 cut(s)	367
MalI	GATC	3 cut(s)	137, 575, 630
MboI	GATC	3 cut(s)	135, 573, 628
MboII	GAAGA	1 cut(s)	638
MflI	RGATCY	1 cut(s)	628
MhlI	GDGCHC	1 cut(s)	912
MlsI	TGGCCA	1 cut(s)	146
MluCI	AATT	3 cut(s)	258, 410, 782
MluNI	TGGCCA	1 cut(s)	146
Mly113I	GGCGCC	1 cut(s)	124
MlyI	GAGTC	2 cut(s)	186, 584
MmeI	TCCRAC	1 cut(s)	43
MnlI	CCTC	9 cut(s)	216, 235, 260, 302, 531, 612, 616, 628, 926
Mox20I	TGGCCA	1 cut(s)	146
MroNI	GCCGGC	1 cut(s)	319
MscI	TGGCCA	1 cut(s)	146
MseI	TTAA	1 cut(s)	903
Msp20I	TGGCCA	1 cut(s)	146
MspA1I	CMGCKG	1 cut(s)	183
MspI	CCGG	5 cut(s)	104, 153, 320, 336, 836
MspR9I	CCNGG	6 cut(s)	104, 462, 569, 789, 836, 899
MvaI	CCWGG	4 cut(s)	462, 569, 789, 899
MvnI	CGCG	1 cut(s)	183
MwoI	GCNNNNNNNGC	2 cut(s)	320, 388
NaeI	GCCGGC	1 cut(s)	321
NarI	GGCGCC	1 cut(s)	124
NciI	CCSGG	2 cut(s)	104, 836
NdeII	GATC	3 cut(s)	135, 573, 628
NgoMIV	GCCGGC	1 cut(s)	319
NlaIII	CATG	5 cut(s)	98, 391, 580, 710, 884
NlaIV	GGNNCC	2 cut(s)	125, 459
NspI	RCATGY	1 cut(s)	391
PaeR7I	CTCGAG	1 cut(s)	535
PagI	TCATGA	1 cut(s)	880
PceI	AGGCCT	2 cut(s)	39, 224
PcsI	WCGNNNNNNNCGW	1 cut(s)	57
PdiI	GCCGGC	1 cut(s)	321
PfeI	GAWTC	2 cut(s)	99, 725
PfoI	TCCNGGA	2 cut(s)	102, 834
PleI	GAGTC	2 cut(s)	186, 584
PluTI	GGCGCC	1 cut(s)	127
PmaCI	CACGTG	2 cut(s)	52, 120
PmlI	CACGTG	2 cut(s)	52, 120
PpsI	GAGTC	2 cut(s)	186, 584
Ppu21I	YACGTR	2 cut(s)	52, 120
PpuMI	RGGWCCY	1 cut(s)	354
Psp124BI	GAGCTC	1 cut(s)	912
Psp5II	RGGWCCY	1 cut(s)	354
Psp6I	CCWGG	4 cut(s)	460, 567, 787, 897
PspCI	CACGTG	2 cut(s)	52, 120
PspGI	CCWGG	4 cut(s)	460, 567, 787, 897
PspN4I	GGNNCC	2 cut(s)	125, 459
PspPI	GGNCC	3 cut(s)	4, 71, 354
PspPPI	RGGWCCY	1 cut(s)	354
PsuI	RGATCY	1 cut(s)	628
RsaI	GTAC	5 cut(s)	33, 116, 326, 524, 653
RsaNI	GTAC	5 cut(s)	32, 115, 325, 523, 652
SacI	GAGCTC	1 cut(s)	912
SacII	CCGCGG	1 cut(s)	184
SaqAI	TTAA	1 cut(s)	903
Sau3AI	GATC	3 cut(s)	135, 573, 628
Sau96I	GGNCC	3 cut(s)	4, 71, 354
SchI	GAGTC	2 cut(s)	186, 584
ScrFI	CCNGG	6 cut(s)	104, 462, 569, 789, 836, 899
SduI	GDGCHC	1 cut(s)	912
SexAI	ACCWGGT	2 cut(s)	460, 897
SfaNI	GCATC	2 cut(s)	256, 487
SfoI	GGCGCC	1 cut(s)	125
Sfr274I	CTCGAG	1 cut(s)	535
Sfr303I	CCGCGG	1 cut(s)	184
SgrBI	CCGCGG	1 cut(s)	184
SinI	GGWCC	3 cut(s)	4, 71, 354
SlaI	CTCGAG	1 cut(s)	535
SmlI	CTYRAG	2 cut(s)	535, 632
SmoI	CTYRAG	2 cut(s)	535, 632
Sse9I	AATT	3 cut(s)	258, 410, 782
SseBI	AGGCCT	2 cut(s)	39, 224
SsiI	CCGC	3 cut(s)	181, 183, 203
SspDI	GGCGCC	1 cut(s)	123
SstI	GAGCTC	1 cut(s)	912
StuI	AGGCCT	2 cut(s)	39, 224
StyD4I	CCNGG	6 cut(s)	102, 460, 567, 787, 834, 897
StyI	CCWWGG	2 cut(s)	357, 611
TaaI	ACNGT	2 cut(s)	31, 508
TaiI	ACGT	3 cut(s)	54, 63, 122
TaqI	TCGA	1 cut(s)	536
TasI	AATT	3 cut(s)	258, 410, 782
TatI	WGTACW	1 cut(s)	522
TfiI	GAWTC	2 cut(s)	99, 725
Tru1I	TTAA	1 cut(s)	903
Tru9I	TTAA	1 cut(s)	903
TscAI	CASTG	2 cut(s)	625, 817
TspDTI	ATGAA	5 cut(s)	716, 742, 809, 869, 897
TspRI	CASTG	2 cut(s)	625, 817
VpaK11BI	GGWCC	3 cut(s)	4, 71, 354
XapI	RAATTY	1 cut(s)	782
XceI	RCATGY	1 cut(s)	391
XhoI	CTCGAG	1 cut(s)	535
XmiI	GTMKAC	1 cut(s)	777
ZraI	GACGTC	1 cut(s)	61

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.