Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr5

Orthogroup ID

OG0001460

Physical Location & Seq

Genomic Coordinates Reverse (-)

29828874 .. 29830657

Gene Structure 1784 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom05g29400.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 936 bp

ATGGAGCAAATCCAGCACAAGTACATAACCGTACAAGGCCTGAAGCTCCACGTCGCCGACATCGGAACAGGTTCCGATGTGGTCGTGTTCCTGCACGGTTTCCCGGAAATTTGGTACTCGTGGCGGCACCAGATGGTTGCGGCGGCCAACGCCGGCTTCCGGGCCATCGCTCCCGACTACCGCGGATACGGACTATCCGACCCTCCTCCCCAATCCGAGAAGACCTCGTACCGTGACCTCACCAGTGACATCCTTGGAATTCTCGATTCCCTTGCTATTCCCAAGGCTTTCCTAATTGCAAAAGATTTTGGAGTTGGGGTAGCGACATTATTTTCTCTTCTGCACCCCGAGAGGGTCGTAGGCGTTGTAACAATAGGATTGCCAATCATGCCACCAGGCCCTCGTAAAACGGGCAACTACCTCCCAGAAGGTTTCTACATTTCAAGATGGCAGGAACCTGGACGTGCAGAGGCTGATTTTGGGCGCCTTGATGCGAAAACAGTTGTTCGGAATGTTTACATCCTTTTTTCCAGAAGCGAAATACCAATAGCTGCAGAAGACCAGGAGATCATGGACCTAGTGGATCCATCTACGCCTCTTCCCCTTTGGTTCAGTGAGGAAGATCTTGCCGCATATGGAGCTTTGTATGAGAAATCGGGTTTCCGAACTGCGCTGCAAGTTCCATACAGGGCAATTAGAGAAGAGTCCTCTGTAGCAGATCCAATAGTTAAAGCTCCGGCGCTTTTTATAATGGGAGGCAAGGATTATGTTAACAAATTTCCAGGGATAGAGGATTACATAAACAGTGGAAAGATCAAAGAGTTTGTCCCCGATTTGGAGATTGTATTTCTTCCCGAAGGAACGCATTTTGTTCAAGAACAGTCGCCTGAAGAGGTCAATCAGCTGATCCTCACCTTCCTCGGAAAGCACATTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000287 GO:0001676 GO:0002532 GO:0002538 GO:0002539 GO:0003008 GO:0003013 GO:0003674 GO:0003824 GO:0004301 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005829 GO:0006082 GO:0006629 GO:0006631 GO:0006793 GO:0006796 GO:0006950 GO:0006952 GO:0006954 GO:0007600 GO:0008015 GO:0008150 GO:0008152 GO:0008217 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0016801 GO:0016803 GO:0019233 GO:0019752 GO:0030258 GO:0032501 GO:0032787 GO:0033559 GO:0042577 GO:0042578 GO:0042579 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043651 GO:0044237 GO:0044238 GO:0044255 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0045777 GO:0046839 GO:0046872 GO:0050877 GO:0050896 GO:0065007 GO:0065008 GO:0071704

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Abhydrolase_1 Abhydrolase_6

Protein Analysis

312

Amino Acids

34.61

Weight (kDa)

5.08

Isoelectric Point (pI)

46.52

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001460)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g05700 FvH4_3g04781 FvH4_3g04781 FvH4_3g04781 FvH4_3g04784 FvH4_3g04784 FvH4_3g04790 FvH4_3g04790 FvH4_3g04790
malus_domestica	MD05G1316900.v1.1 MD05G1317400.v1.1 MD10G1297200.v1.1
prunus_persica	Prupe.4G043200_v2.0.a1 Prupe.4G043200_v2.0.a1
pyrus_communis	pycom05g29400 pycom10g25010 pycom10g25060
rosa_chinensis	RchiOBHm_Chr5g0007821 RchiOBHm_Chr5g0007841 RchiOBHm_Chr5g0007871 RchiOBHm_Chr5g0007881
rosa_laevigata	RLG00000031499 RLG00000031500 RLG00000031501
rosa_multiflora	Rmu_co8472583.1_g000001 Rmu_sc0005098.1_g000007 Rmu_sc0005098.1_g000012
rosa_roxburghii	Rroxscaffold_1G00068550 Rroxscaffold_1G00068560 Rroxscaffold_1G00068580
rosa_rugosa	Rorug04G0430600 Rorug04G0430600 Rorug04G0430600 Rorug04G0430600 Rorug04G0430700 Rorug04G0430800
rosa_samantha	Rh5AG059200 Rh5AG059300 Rh5AG059600 Rh5AG059700 Rh5BG056200 Rh5BG056300 Rh5BG056400 Rh5BG056500 Rh5CG066000 Rh5CG066100 Rh5CG066400 Rh5DG055300 Rh5DG055500
rosa_wichuraiana	Rw5G006050 Rw5G006060 Rw5G006070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	749
AccB1I	GGYRCC	2 cut(s)	126, 483
AccII	CGCG	1 cut(s)	183
AciI	CCGC	6 cut(s)	124, 140, 143, 181, 183, 630
AclWI	GGATC	4 cut(s)	578, 591, 713, 901
AcoI	YGGCCR	1 cut(s)	144
AcsI	RAATTY	3 cut(s)	108, 258, 776
AcuI	CTGAAG	2 cut(s)	62, 909
AcyI	GRCGYC	1 cut(s)	484
AfaI	GTAC	4 cut(s)	23, 33, 116, 230
AfiI	CCNNNNNNNGG	3 cut(s)	159, 352, 835
AgsI	TTSAA	2 cut(s)	444, 875
AjiI	CACGTC	2 cut(s)	52, 464
AjnI	CCWGG	4 cut(s)	394, 457, 561, 781
AjuI	GAANNNNNNNTTGG	2 cut(s)	140, 172
AluBI	AGCT	5 cut(s)	46, 551, 641, 734, 904
AluI	AGCT	5 cut(s)	46, 551, 641, 734, 904
AlwI	GGATC	4 cut(s)	578, 591, 713, 901
AlwNI	CAGNNNCTG	1 cut(s)	473
Ama87I	CYCGRG	1 cut(s)	347
AoxI	GGCC	4 cut(s)	37, 144, 162, 397
ApeKI	GCWGC	2 cut(s)	551, 673
ApoI	RAATTY	3 cut(s)	108, 258, 776
ArsI	GACNNNNNNTTYG	2 cut(s)	810, 842
Asp700I	GAANNNNTTC	1 cut(s)	70
AspLEI	GCGC	3 cut(s)	486, 673, 742
AspS9I	GGNCC	3 cut(s)	162, 398, 574
AsuC2I	CCSGG	2 cut(s)	104, 161
AsuHPI	GGTGA	2 cut(s)	232, 904
AvaI	CYCGRG	1 cut(s)	347
AvaII	GGWCC	1 cut(s)	574
BamHI	GGATCC	1 cut(s)	583
BanI	GGYRCC	2 cut(s)	126, 483
BarI	GAAGNNNNNNTAC	2 cut(s)	212, 244
BauI	CACGAG	1 cut(s)	118
BbsI	GAAGAC	2 cut(s)	227, 564
BbvI	GCAGC	2 cut(s)	538, 660
BccI	CCATC	4 cut(s)	127, 173, 441, 595
BcgI	CGANNNNNNTGC	2 cut(s)	254, 288
BciT130I	CCWGG	4 cut(s)	396, 459, 563, 783
BciVI	GTATCC	1 cut(s)	179
BcnI	CCSGG	2 cut(s)	104, 161
BfaI	CTAG	1 cut(s)	578
BfmI	CTRYAG	2 cut(s)	552, 711
BfoI	RGCGCY	2 cut(s)	487, 743
BfuI	GTATCC	1 cut(s)	179
BglII	AGATCT	1 cut(s)	622
BisI	GCNGC	6 cut(s)	125, 141, 144, 552, 630, 674
BlsI	GCNGC	6 cut(s)	126, 142, 145, 553, 631, 675
Bme1390I	CCNGG	6 cut(s)	104, 161, 396, 459, 563, 783
Bme18I	GGWCC	1 cut(s)	574
BmeT110I	CYCGRG	1 cut(s)	347
BmgBI	CACGTC	2 cut(s)	52, 464
BmgT120I	GGNCC	3 cut(s)	162, 398, 574
BmiI	GGNNCC	5 cut(s)	73, 128, 456, 485, 585
BmrFI	CCNGG	6 cut(s)	104, 161, 396, 459, 563, 783
BmsI	GCATC	1 cut(s)	481
BpiI	GAAGAC	2 cut(s)	227, 564
BplI	GAGNNNNNCTC	2 cut(s)	209, 241
BpuMI	CCSGG	2 cut(s)	104, 161
BsaHI	GRCGYC	1 cut(s)	484
BsaJI	CCNNGG	5 cut(s)	181, 253, 282, 782, 919
BsaXI	ACNNNNNCTCC	2 cut(s)	303, 333
Bsc4I	CCNNNNNNNGG	3 cut(s)	159, 352, 835
Bse118I	RCCGGY	1 cut(s)	152
Bse1I	ACTGG	1 cut(s)	243
BseBI	CCWGG	4 cut(s)	396, 459, 563, 783
BseDI	CCNNGG	5 cut(s)	181, 253, 282, 782, 919
BseGI	GGATG	2 cut(s)	249, 519
BseLI	CCNNNNNNNGG	3 cut(s)	159, 352, 835
BseNI	ACTGG	1 cut(s)	243
BseRI	GAGGAG	1 cut(s)	195
BseXI	GCAGC	2 cut(s)	538, 660
BsgI	GTGCAG	3 cut(s)	77, 326, 486
Bsh1236I	CGCG	1 cut(s)	183
BshFI	GGCC	4 cut(s)	39, 146, 164, 399
BshNI	GGYRCC	2 cut(s)	126, 483
BsiHKCI	CYCGRG	1 cut(s)	347
BsiSI	CCGG	4 cut(s)	104, 153, 160, 737
BslFI	GGGAC	1 cut(s)	812
BslI	CCNNNNNNNGG	3 cut(s)	159, 352, 835
BsmFI	GGGAC	1 cut(s)	812
BsnI	GGCC	4 cut(s)	39, 146, 164, 399
BsoBI	CYCGRG	1 cut(s)	347
Bsp143I	GATC	6 cut(s)	567, 583, 622, 718, 813, 906
BspACI	CCGC	6 cut(s)	124, 140, 143, 181, 183, 630
BspANI	GGCC	4 cut(s)	39, 146, 164, 399
BspFNI	CGCG	1 cut(s)	183
BspLI	GGNNCC	5 cut(s)	73, 128, 456, 485, 585
BspMAI	CTGCAG	1 cut(s)	556
BspPI	GGATC	4 cut(s)	578, 591, 713, 901
BspT107I	GGYRCC	2 cut(s)	126, 483
BsrFI	RCCGGY	1 cut(s)	152
BsrI	ACTGG	1 cut(s)	243
BssAI	RCCGGY	1 cut(s)	152
BssECI	CCNNGG	5 cut(s)	181, 253, 282, 782, 919
BssMI	GATC	6 cut(s)	567, 583, 622, 718, 813, 906
BssNI	GRCGYC	1 cut(s)	484
BssSI	CACGAG	1 cut(s)	118
BssT1I	CCWWGG	2 cut(s)	253, 282
Bst2BI	CACGAG	1 cut(s)	118
Bst2UI	CCWGG	4 cut(s)	396, 459, 563, 783
Bst4CI	ACNGT	6 cut(s)	31, 98, 233, 502, 806, 882
Bst6I	CTCTTC	4 cut(s)	342, 603, 696, 885
BstACI	GRCGYC	1 cut(s)	484
BstC8I	GCNNGC	1 cut(s)	154
BstDSI	CCRYGG	1 cut(s)	181
BstF5I	GGATG	2 cut(s)	249, 519
BstFNI	CGCG	1 cut(s)	183
BstH2I	RGCGCY	2 cut(s)	487, 743
BstHHI	GCGC	3 cut(s)	486, 673, 742
BstKTI	GATC	6 cut(s)	570, 586, 625, 721, 816, 909
BstMBI	GATC	6 cut(s)	567, 583, 622, 718, 813, 906
BstMWI	GCNNNNNNNGC	4 cut(s)	13, 149, 388, 638
BstNI	CCWGG	4 cut(s)	396, 459, 563, 783
BstSCI	CCNGG	6 cut(s)	102, 159, 394, 457, 561, 781
BstSFI	CTRYAG	2 cut(s)	552, 711
BstUI	CGCG	1 cut(s)	183
BstV1I	GCAGC	2 cut(s)	538, 660
BstV2I	GAAGAC	2 cut(s)	227, 564
BstX2I	RGATCY	3 cut(s)	583, 622, 718
BstYI	RGATCY	3 cut(s)	583, 622, 718
BsuI	GTATCC	1 cut(s)	179
BsuRI	GGCC	4 cut(s)	39, 146, 164, 399
BtgI	CCRYGG	1 cut(s)	181
BtgZI	GCGATG	1 cut(s)	151
BtrI	CACGTC	2 cut(s)	52, 464
BtsCI	GGATG	2 cut(s)	249, 519
BtsIMutI	CAGTG	3 cut(s)	250, 619, 811
Cac8I	GCNNGC	1 cut(s)	154
CaiI	CAGNNNCTG	1 cut(s)	473
CfoI	GCGC	3 cut(s)	486, 673, 742
Cfr10I	RCCGGY	1 cut(s)	152
Cfr13I	GGNCC	3 cut(s)	162, 398, 574
Cfr42I	CCGCGG	1 cut(s)	184
Csp6I	GTAC	4 cut(s)	22, 32, 115, 229
CviAII	CATG	2 cut(s)	388, 571
CviQI	GTAC	4 cut(s)	22, 32, 115, 229
DinI	GGCGCC	1 cut(s)	485
DpnI	GATC	6 cut(s)	569, 585, 624, 720, 815, 908
DpnII	GATC	6 cut(s)	567, 583, 622, 718, 813, 906
EaeI	YGGCCR	1 cut(s)	144
Eam1104I	CTCTTC	4 cut(s)	342, 603, 696, 885
EarI	CTCTTC	4 cut(s)	342, 603, 696, 885
Eco130I	CCWWGG	2 cut(s)	253, 282
Eco147I	AGGCCT	1 cut(s)	39
Eco47I	GGWCC	1 cut(s)	574
Eco57I	CTGAAG	2 cut(s)	62, 909
Eco88I	CYCGRG	1 cut(s)	347
EcoO109I	RGGNCCY	1 cut(s)	398
EcoRI	GAATTC	1 cut(s)	258
EcoRII	CCWGG	4 cut(s)	394, 457, 561, 781
EcoT14I	CCWWGG	2 cut(s)	253, 282
EgeI	GGCGCC	1 cut(s)	485
EheI	GGCGCC	1 cut(s)	485
ErhI	CCWWGG	2 cut(s)	253, 282
FaeI	CATG	2 cut(s)	391, 574
FaqI	GGGAC	1 cut(s)	812
FatI	CATG	2 cut(s)	387, 570
FauNDI	CATATG	1 cut(s)	634
Fnu4HI	GCNGC	6 cut(s)	125, 141, 144, 552, 630, 674
FokI	GGATG	2 cut(s)	236, 506
Fsp4HI	GCNGC	6 cut(s)	125, 141, 144, 552, 630, 674
FspBI	CTAG	1 cut(s)	578
GlaI	GCGC	3 cut(s)	485, 672, 741
GluI	GCNGC	6 cut(s)	125, 141, 144, 552, 630, 674
HaeII	RGCGCY	2 cut(s)	487, 743
HaeIII	GGCC	4 cut(s)	39, 146, 164, 399
HapII	CCGG	4 cut(s)	104, 153, 160, 737
HhaI	GCGC	3 cut(s)	486, 673, 742
Hin1I	GRCGYC	1 cut(s)	484
Hin1II	CATG	2 cut(s)	391, 574
Hin6I	GCGC	3 cut(s)	484, 671, 740
HinP1I	GCGC	3 cut(s)	484, 671, 740
HincII	GTYRAC	1 cut(s)	772
HindII	GTYRAC	1 cut(s)	772
HinfI	GANTC	2 cut(s)	266, 704
HpaI	GTTAAC	1 cut(s)	772
HpaII	CCGG	4 cut(s)	104, 153, 160, 737
HphI	GGTGA	2 cut(s)	232, 904
Hpy166II	GTNNAC	2 cut(s)	517, 772
Hpy188I	TCNGA	7 cut(s)	65, 76, 199, 217, 510, 665, 923
Hpy188III	TCNNGA	6 cut(s)	173, 263, 444, 531, 854, 875
Hpy8I	GTNNAC	2 cut(s)	517, 772
Hpy99I	CGWCG	1 cut(s)	56
HpyAV	CCTTC	3 cut(s)	422, 851, 925
HpyCH4III	ACNGT	6 cut(s)	31, 98, 233, 502, 806, 882
HpyCH4IV	ACGT	2 cut(s)	51, 463
HpyCH4V	TGCA	6 cut(s)	94, 299, 343, 467, 554, 676
HpyF10VI	GCNNNNNNNGC	4 cut(s)	13, 149, 388, 638
HpySE526I	ACGT	2 cut(s)	51, 463
Hsp92I	GRCGYC	1 cut(s)	484
Hsp92II	CATG	2 cut(s)	391, 574
HspAI	GCGC	3 cut(s)	484, 671, 740
KasI	GGCGCC	1 cut(s)	483
KroI	GCCGGC	1 cut(s)	152
KroNI	GCCGGC	1 cut(s)	154
KspAI	GTTAAC	1 cut(s)	772
KspI	CCGCGG	1 cut(s)	184
Kzo9I	GATC	6 cut(s)	567, 583, 622, 718, 813, 906
LmnI	GCTCC	5 cut(s)	4, 51, 175, 638, 739
Lsp1109I	GCAGC	2 cut(s)	538, 660
LweI	GCATC	1 cut(s)	481
MaeI	CTAG	1 cut(s)	578
MaeII	ACGT	2 cut(s)	51, 463
MaeIII	GTNAC	3 cut(s)	233, 245, 367
MalI	GATC	6 cut(s)	569, 585, 624, 720, 815, 908
MboI	GATC	6 cut(s)	567, 583, 622, 718, 813, 906
MboII	GAAGA	8 cut(s)	232, 329, 569, 590, 632, 713, 842, 902
MflI	RGATCY	3 cut(s)	583, 622, 718
MluCI	AATT	5 cut(s)	108, 258, 294, 693, 776
Mly113I	GGCGCC	1 cut(s)	484
MlyI	GAGTC	1 cut(s)	713
MmeI	TCCRAC	1 cut(s)	222
MroNI	GCCGGC	1 cut(s)	152
MroXI	GAANNNNTTC	1 cut(s)	70
MseI	TTAA	2 cut(s)	729, 771
MspA1I	CMGCKG	2 cut(s)	183, 904
MspI	CCGG	4 cut(s)	104, 153, 160, 737
MspR9I	CCNGG	6 cut(s)	104, 161, 396, 459, 563, 783
MvaI	CCWGG	4 cut(s)	396, 459, 563, 783
MvnI	CGCG	1 cut(s)	183
MwoI	GCNNNNNNNGC	4 cut(s)	13, 149, 388, 638
NaeI	GCCGGC	1 cut(s)	154
NarI	GGCGCC	1 cut(s)	484
NciI	CCSGG	2 cut(s)	104, 161
NdeI	CATATG	1 cut(s)	634
NdeII	GATC	6 cut(s)	567, 583, 622, 718, 813, 906
NgoMIV	GCCGGC	1 cut(s)	152
NlaIII	CATG	2 cut(s)	391, 574
NlaIV	GGNNCC	5 cut(s)	73, 128, 456, 485, 585
NmuCI	GTSAC	2 cut(s)	233, 245
PceI	AGGCCT	1 cut(s)	39
PcsI	WCGNNNNNNNCGW	1 cut(s)	195
PdiI	GCCGGC	1 cut(s)	154
PdmI	GAANNNNTTC	1 cut(s)	70
PfeI	GAWTC	1 cut(s)	266
PfoI	TCCNGGA	1 cut(s)	102
PkrI	GCNGC	6 cut(s)	126, 142, 145, 553, 631, 675
PleI	GAGTC	1 cut(s)	712
PluTI	GGCGCC	1 cut(s)	487
PpsI	GAGTC	1 cut(s)	712
PsiI	TTATAA	1 cut(s)	749
Psp6I	CCWGG	4 cut(s)	394, 457, 561, 781
PspGI	CCWGG	4 cut(s)	394, 457, 561, 781
PspN4I	GGNNCC	5 cut(s)	73, 128, 456, 485, 585
PspPI	GGNCC	3 cut(s)	162, 398, 574
PstI	CTGCAG	1 cut(s)	556
PstNI	CAGNNNCTG	1 cut(s)	473
PsuI	RGATCY	3 cut(s)	583, 622, 718
PvuII	CAGCTG	1 cut(s)	904
RsaI	GTAC	4 cut(s)	23, 33, 116, 230
RsaNI	GTAC	4 cut(s)	22, 32, 115, 229
SacII	CCGCGG	1 cut(s)	184
SaqAI	TTAA	2 cut(s)	729, 771
SatI	GCNGC	6 cut(s)	125, 141, 144, 552, 630, 674
Sau3AI	GATC	6 cut(s)	567, 583, 622, 718, 813, 906
Sau96I	GGNCC	3 cut(s)	162, 398, 574
SchI	GAGTC	1 cut(s)	713
ScrFI	CCNGG	6 cut(s)	104, 161, 396, 459, 563, 783
SfaNI	GCATC	1 cut(s)	481
SfcI	CTRYAG	2 cut(s)	552, 711
SfoI	GGCGCC	1 cut(s)	485
Sfr303I	CCGCGG	1 cut(s)	184
SgrBI	CCGCGG	1 cut(s)	184
SinI	GGWCC	1 cut(s)	574
Sse9I	AATT	5 cut(s)	108, 258, 294, 693, 776
SseBI	AGGCCT	1 cut(s)	39
SsiI	CCGC	6 cut(s)	124, 140, 143, 181, 183, 630
SspDI	GGCGCC	1 cut(s)	483
SspMI	CTAG	1 cut(s)	578
StuI	AGGCCT	1 cut(s)	39
StyD4I	CCNGG	6 cut(s)	102, 159, 394, 457, 561, 781
StyI	CCWWGG	2 cut(s)	253, 282
TaaI	ACNGT	6 cut(s)	31, 98, 233, 502, 806, 882
TaiI	ACGT	2 cut(s)	54, 466
TaqI	TCGA	1 cut(s)	264
TasI	AATT	5 cut(s)	108, 258, 294, 693, 776
TatI	WGTACW	1 cut(s)	21
TauI	GCSGC	4 cut(s)	127, 143, 146, 632
TfiI	GAWTC	1 cut(s)	266
Tru1I	TTAA	2 cut(s)	729, 771
Tru9I	TTAA	2 cut(s)	729, 771
TscAI	CASTG	3 cut(s)	250, 619, 811
TseFI	GTSAC	2 cut(s)	233, 245
TseI	GCWGC	2 cut(s)	551, 673
Tsp45I	GTSAC	2 cut(s)	233, 245
TspGWI	ACGGA	1 cut(s)	204
TspRI	CASTG	3 cut(s)	250, 619, 811
VpaK11BI	GGWCC	1 cut(s)	574
XapI	RAATTY	3 cut(s)	108, 258, 776
XmnI	GAANNNNTTC	1 cut(s)	70
XspI	CTAG	1 cut(s)	578

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.