FvH4_3g04790

Bifunctional epoxide hydrolase 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0001460
Physical Location & Seq
Forward (+)
2762266 .. 2764296
2031 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g04790.t2

Sequence Viewer

Length: 939 bp
ATGGACCAGATTCAGCACAAGTACATAGCCGTACATGGCCTGAACCTCCACGTCGCCGACATCGGAACCGGTCCCAATGTGGTCATGTTCCTGCACGGATTCCCAGAAATATGGTACTCGTGGCGCCACCAGATGATCGCTGCCGCCGGCGCCGGCTTCCGGGCCATCGCCCCCGACTACCGTGGACATGGTCTTTCCGACTTGCCGCCGGAACCCGAGAAGGCCTCCTACCGTGACCTCGTCAATGACATCCTTGCAATTCTTGAAGCCCTTGCTATCACTAAGTTTTTCGTGGTTGCAAAAGATTATGGAGCTTGGCCAGCATACATACTTGCTCTTCTTTACCCAGATAGGATCTTGGGAGTTGTAACATTGGGAGTGCCATACTTACCAGCTTCAAGTCGTCCTAGTTTCGCAGAATCCCTCCCTGAAGGTTTCTACATCTCAAGATTTCATGAACCAGGCAGGGCTGAAGCAGATTTCGGGCGCTTTGATGCGACAACAGTTTTTCGGAATATTTACATCCTTTTCTCCAGAAGCGAAATCCCAATAGCTGCAGAAAACCAGGAGATCATGGACTTGGTTGACCCCTCTACACCTCTTCCCCCTTGGTTATCTGAGGAAGATCTTCAAGCATATGGAGATTCATATGAGAGAACAGGTTTCCAGACTGCATTGCAGATCACATATAGGTCCAAGTCAGACGACATGGGCATAACAGAGCTAATTGTTAAAGTTCCGGTGCTTGTTATAGTGGGACAGAAGGATTACTTTCTAAAGTTTCCGGGGATAGAGGACTCTATAACCAGTGGAAAGGTGAAGGAGTTTGCAACTGATTTGGAGCTTGTAGATATGCCTGAAGGAACACATTTTGCTCAAGAACAATTTCCTGACCAGTTCAATCAGATAATGCTCACCTTCCTTAAAAAGCACATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000287 GO:0001676 GO:0002532 GO:0002538 GO:0002539 GO:0003008 GO:0003013 GO:0003018 GO:0003674 GO:0003824 GO:0004301 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005782 GO:0005829 GO:0006082 GO:0006605 GO:0006625 GO:0006629 GO:0006631 GO:0006633 GO:0006690 GO:0006725 GO:0006793 GO:0006796 GO:0006805 GO:0006810 GO:0006873 GO:0006874 GO:0006875 GO:0006886 GO:0006950 GO:0006952 GO:0006954 GO:0006996 GO:0007031 GO:0007600 GO:0008015 GO:0008104 GO:0008150 GO:0008152 GO:0008217 GO:0008610 GO:0009056 GO:0009058 GO:0009410 GO:0009636 GO:0009810 GO:0009893 GO:0009987 GO:0010468 GO:0010604 GO:0010628 GO:0015031 GO:0015643 GO:0015833 GO:0016043 GO:0016053 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0016801 GO:0016803 GO:0017144 GO:0018904 GO:0019216 GO:0019218 GO:0019222 GO:0019233 GO:0019369 GO:0019373 GO:0019439 GO:0019725 GO:0019752 GO:0030003 GO:0030258 GO:0031907 GO:0031974 GO:0032501 GO:0032787 GO:0033036 GO:0033365 GO:0033559 GO:0034613 GO:0035150 GO:0035296 GO:0042221 GO:0042577 GO:0042578 GO:0042579 GO:0042592 GO:0042632 GO:0042759 GO:0042802 GO:0042803 GO:0042886 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043574 GO:0043651 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0045777 GO:0046272 GO:0046394 GO:0046483 GO:0046839 GO:0046872 GO:0046907 GO:0046983 GO:0048518 GO:0048878 GO:0050789 GO:0050801 GO:0050877 GO:0050880 GO:0050896 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051716 GO:0055065 GO:0055074 GO:0055080 GO:0055082 GO:0055088 GO:0055092 GO:0060255 GO:0062012 GO:0065007 GO:0065008 GO:0070013 GO:0070727 GO:0070887 GO:0071466 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072330 GO:0072503 GO:0072507 GO:0072593 GO:0072594 GO:0072662 GO:0072663 GO:0080090 GO:0090066 GO:0090181 GO:0097176 GO:0097746 GO:0097755 GO:0098771 GO:1900673 GO:1901360 GO:1901361 GO:1901568 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

313

Amino Acids

35.08

Weight (kDa)

4.91

Isoelectric Point (pI)

42.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 26 - 145 1.7e-21 alpha/beta hydrolase fold
Hydrolase_4 PF12146 27 - 126 8.7e-08 Serine aminopeptidase, S33
Abhydrolase_6 PF12697 28 - 168 2.4e-12 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 123, 149
AciI CCGC 2 cut(s) 144, 206
AclWI GGATC 1 cut(s) 362
AcoI YGGCCR 1 cut(s) 317
AcuI CTGAAG 3 cut(s) 450, 492, 879
AcyI GRCGYC 2 cut(s) 124, 150
AfaI GTAC 3 cut(s) 23, 33, 116
AfiI CCNNNNNNNGG 1 cut(s) 159
AgeI ACCGGT 1 cut(s) 68
AgsI TTSAA 4 cut(s) 266, 399, 632, 901
AjiI CACGTC 1 cut(s) 52
AjnI CCWGG 2 cut(s) 460, 564
AluBI AGCT 5 cut(s) 314, 395, 554, 724, 844
AluI AGCT 5 cut(s) 314, 395, 554, 724, 844
AlwI GGATC 1 cut(s) 362
Ama87I CYCGRG 1 cut(s) 215
AoxI GGCC 4 cut(s) 37, 162, 222, 317
ApeKI GCWGC 2 cut(s) 140, 554
AsiGI ACCGGT 1 cut(s) 68
Asp700I GAANNNNTTC 2 cut(s) 627, 885
AspLEI GCGC 3 cut(s) 126, 152, 489
AspS9I GGNCC 4 cut(s) 4, 71, 162, 693
AsuC2I CCSGG 2 cut(s) 161, 786
AsuHPI GGTGA 2 cut(s) 829, 907
AvaI CYCGRG 1 cut(s) 215
AvaII GGWCC 3 cut(s) 4, 71, 693
BalI TGGCCA 1 cut(s) 319
BanI GGYRCC 2 cut(s) 123, 149
BarI GAAGNNNNNNTAC 2 cut(s) 212, 244
BauI CACGAG 1 cut(s) 118
BbvI GCAGC 2 cut(s) 127, 541
BccI CCATC 1 cut(s) 173
BceAI ACGGC 1 cut(s) 14
BciT130I CCWGG 2 cut(s) 462, 566
BcnI CCSGG 2 cut(s) 161, 786
BfaI CTAG 1 cut(s) 408
BfmI CTRYAG 1 cut(s) 555
BfoI RGCGCY 3 cut(s) 127, 153, 490
BglII AGATCT 1 cut(s) 625
BisI GCNGC 4 cut(s) 141, 144, 206, 555
BlsI GCNGC 4 cut(s) 142, 145, 207, 556
Bme1390I CCNGG 4 cut(s) 161, 462, 566, 786
Bme18I GGWCC 3 cut(s) 4, 71, 693
BmeT110I CYCGRG 1 cut(s) 215
BmgBI CACGTC 1 cut(s) 52
BmgT120I GGNCC 4 cut(s) 4, 71, 162, 693
BmiI GGNNCC 5 cut(s) 67, 73, 125, 151, 213
BmrFI CCNGG 4 cut(s) 161, 462, 566, 786
BmsI GCATC 1 cut(s) 484
BplI GAGNNNNNCTC 2 cut(s) 209, 241
BpmI CTGGAG 1 cut(s) 517
BpuEI CTTGAG 2 cut(s) 430, 861
BpuMI CCSGG 2 cut(s) 161, 786
BsaHI GRCGYC 2 cut(s) 124, 150
BsaJI CCNNGG 3 cut(s) 181, 608, 785
BsaWI WCCGGW 2 cut(s) 68, 739
Bsc4I CCNNNNNNNGG 1 cut(s) 159
Bse118I RCCGGY 3 cut(s) 68, 146, 152
Bse1I ACTGG 2 cut(s) 807, 895
Bse3DI GCAATG 1 cut(s) 674
BseBI CCWGG 2 cut(s) 462, 566
BseDI CCNNGG 3 cut(s) 181, 608, 785
BseGI GGATG 2 cut(s) 249, 522
BseLI CCNNNNNNNGG 1 cut(s) 159
BseMI GCAATG 1 cut(s) 674
BseMII CTCAG 1 cut(s) 609
BseNI ACTGG 2 cut(s) 807, 895
BseXI GCAGC 2 cut(s) 127, 541
BsgI GTGCAG 1 cut(s) 77
BshFI GGCC 4 cut(s) 39, 164, 224, 319
BshNI GGYRCC 2 cut(s) 123, 149
BshTI ACCGGT 1 cut(s) 68
BsiHKCI CYCGRG 1 cut(s) 215
BsiSI CCGG 7 cut(s) 69, 147, 153, 160, 209, 740, 785
BslFI GGGAC 2 cut(s) 57, 771
BslI CCNNNNNNNGG 1 cut(s) 159
BsmFI GGGAC 2 cut(s) 57, 771
BsnI GGCC 4 cut(s) 39, 164, 224, 319
BsoBI CYCGRG 1 cut(s) 215
Bsp143I GATC 5 cut(s) 135, 354, 570, 625, 681
BspACI CCGC 2 cut(s) 144, 206
BspANI GGCC 4 cut(s) 39, 164, 224, 319
BspCNI CTCAG 1 cut(s) 610
BspHI TCATGA 1 cut(s) 454
BspLI GGNNCC 5 cut(s) 67, 73, 125, 151, 213
BspMAI CTGCAG 1 cut(s) 559
BspPI GGATC 1 cut(s) 362
BspQI GCTCTTC 1 cut(s) 342
BspT107I GGYRCC 2 cut(s) 123, 149
BsrDI GCAATG 1 cut(s) 674
BsrFI RCCGGY 3 cut(s) 68, 146, 152
BsrI ACTGG 2 cut(s) 807, 895
BssAI RCCGGY 3 cut(s) 68, 146, 152
BssECI CCNNGG 3 cut(s) 181, 608, 785
BssMI GATC 5 cut(s) 135, 354, 570, 625, 681
BssNI GRCGYC 2 cut(s) 124, 150
BssSI CACGAG 1 cut(s) 118
BssT1I CCWWGG 1 cut(s) 608
Bst2BI CACGAG 1 cut(s) 118
Bst2UI CCWGG 2 cut(s) 462, 566
Bst4CI ACNGT 3 cut(s) 182, 233, 505
Bst6I CTCTTC 2 cut(s) 342, 606
BstACI GRCGYC 2 cut(s) 124, 150
BstC8I GCNNGC 3 cut(s) 148, 154, 321
BstDEI CTNAG 2 cut(s) 282, 618
BstDSI CCRYGG 1 cut(s) 181
BstF5I GGATG 2 cut(s) 249, 522
BstH2I RGCGCY 3 cut(s) 127, 153, 490
BstHHI GCGC 3 cut(s) 126, 152, 489
BstKTI GATC 5 cut(s) 138, 357, 573, 628, 684
BstMBI GATC 5 cut(s) 135, 354, 570, 625, 681
BstMWI GCNNNNNNNGC 2 cut(s) 149, 320
BstNI CCWGG 2 cut(s) 462, 566
BstSCI CCNGG 4 cut(s) 159, 460, 564, 784
BstSFI CTRYAG 1 cut(s) 555
BstV1I GCAGC 2 cut(s) 127, 541
BstX2I RGATCY 2 cut(s) 354, 625
BstXI CCANNNNNNTGG 1 cut(s) 111
BstYI RGATCY 2 cut(s) 354, 625
BsuRI GGCC 4 cut(s) 39, 164, 224, 319
BtgI CCRYGG 1 cut(s) 181
BtgZI GCGATG 1 cut(s) 151
BtrI CACGTC 1 cut(s) 52
BtsCI GGATG 2 cut(s) 249, 522
BtsIMutI CAGTG 1 cut(s) 814
Cac8I GCNNGC 3 cut(s) 148, 154, 321
CciI TCATGA 1 cut(s) 454
CfoI GCGC 3 cut(s) 126, 152, 489
Cfr10I RCCGGY 3 cut(s) 68, 146, 152
Cfr13I GGNCC 4 cut(s) 4, 71, 162, 693
Csp6I GTAC 3 cut(s) 22, 32, 115
CspAI ACCGGT 1 cut(s) 68
CviAII CATG 6 cut(s) 35, 85, 188, 455, 574, 709
CviQI GTAC 3 cut(s) 22, 32, 115
DdeI CTNAG 2 cut(s) 282, 618
DinI GGCGCC 2 cut(s) 125, 151
DpnI GATC 5 cut(s) 137, 356, 572, 627, 683
DpnII GATC 5 cut(s) 135, 354, 570, 625, 681
EaeI YGGCCR 1 cut(s) 317
Eam1104I CTCTTC 2 cut(s) 342, 606
EarI CTCTTC 2 cut(s) 342, 606
Eco130I CCWWGG 1 cut(s) 608
Eco147I AGGCCT 1 cut(s) 224
Eco47I GGWCC 3 cut(s) 4, 71, 693
Eco57I CTGAAG 3 cut(s) 450, 492, 879
Eco88I CYCGRG 1 cut(s) 215
EcoRII CCWGG 2 cut(s) 460, 564
EcoT14I CCWWGG 1 cut(s) 608
EgeI GGCGCC 2 cut(s) 125, 151
EheI GGCGCC 2 cut(s) 125, 151
ErhI CCWWGG 1 cut(s) 608
FaeI CATG 6 cut(s) 38, 88, 191, 458, 577, 712
FalI AAGNNNNNCTT 2 cut(s) 755, 787
FaqI GGGAC 2 cut(s) 57, 771
FatI CATG 6 cut(s) 34, 84, 187, 454, 573, 708
FauNDI CATATG 2 cut(s) 637, 649
Fnu4HI GCNGC 4 cut(s) 141, 144, 206, 555
FokI GGATG 2 cut(s) 236, 509
Fsp4HI GCNGC 4 cut(s) 141, 144, 206, 555
FspBI CTAG 1 cut(s) 408
GlaI GCGC 3 cut(s) 125, 151, 488
GluI GCNGC 4 cut(s) 141, 144, 206, 555
GsuI CTGGAG 1 cut(s) 517
HaeII RGCGCY 3 cut(s) 127, 153, 490
HaeIII GGCC 4 cut(s) 39, 164, 224, 319
HapII CCGG 7 cut(s) 69, 147, 153, 160, 209, 740, 785
HhaI GCGC 3 cut(s) 126, 152, 489
Hin1I GRCGYC 2 cut(s) 124, 150
Hin1II CATG 6 cut(s) 38, 88, 191, 458, 577, 712
Hin6I GCGC 3 cut(s) 124, 150, 487
HinP1I GCGC 3 cut(s) 124, 150, 487
HincII GTYRAC 1 cut(s) 586
HindII GTYRAC 1 cut(s) 586
HinfI GANTC 5 cut(s) 10, 99, 419, 644, 797
HpaII CCGG 7 cut(s) 69, 147, 153, 160, 209, 740, 785
HphI GGTGA 2 cut(s) 829, 907
Hpy166II GTNNAC 2 cut(s) 185, 586
Hpy188I TCNGA 7 cut(s) 65, 199, 513, 619, 703, 906, 938
Hpy188III TCNNGA 7 cut(s) 263, 447, 455, 534, 667, 878, 890
Hpy8I GTNNAC 2 cut(s) 185, 586
Hpy99I CGWCG 1 cut(s) 56
HpyAV CCTTC 6 cut(s) 214, 425, 757, 814, 854, 928
HpyCH4III ACNGT 3 cut(s) 182, 233, 505
HpyCH4IV ACGT 1 cut(s) 51
HpyCH4V TGCA 7 cut(s) 94, 257, 299, 557, 674, 679, 830
HpyF10VI GCNNNNNNNGC 2 cut(s) 149, 320
HpyF3I CTNAG 2 cut(s) 282, 618
HpySE526I ACGT 1 cut(s) 51
Hsp92I GRCGYC 2 cut(s) 124, 150
Hsp92II CATG 6 cut(s) 38, 88, 191, 458, 577, 712
HspAI GCGC 3 cut(s) 124, 150, 487
KasI GGCGCC 2 cut(s) 123, 149
KroI GCCGGC 2 cut(s) 146, 152
KroNI GCCGGC 2 cut(s) 148, 154
Kzo9I GATC 5 cut(s) 135, 354, 570, 625, 681
LguI GCTCTTC 1 cut(s) 342
LmnI GCTCC 2 cut(s) 311, 841
Lsp1109I GCAGC 2 cut(s) 127, 541
LweI GCATC 1 cut(s) 484
MaeI CTAG 1 cut(s) 408
MaeII ACGT 1 cut(s) 51
MaeIII GTNAC 2 cut(s) 233, 367
MalI GATC 5 cut(s) 137, 356, 572, 627, 683
MboI GATC 5 cut(s) 135, 354, 570, 625, 681
MboII GAAGA 4 cut(s) 329, 593, 620, 635
MflI RGATCY 2 cut(s) 354, 625
MlsI TGGCCA 1 cut(s) 319
MluCI AATT 3 cut(s) 258, 726, 884
MluNI TGGCCA 1 cut(s) 319
Mly113I GGCGCC 2 cut(s) 124, 150
MlyI GAGTC 1 cut(s) 791
MmeI TCCRAC 1 cut(s) 222
MnlI CCTC 8 cut(s) 56, 235, 248, 434, 601, 609, 613, 787
Mox20I TGGCCA 1 cut(s) 319
MreI CGCCGGCG 1 cut(s) 146
MroNI GCCGGC 2 cut(s) 146, 152
MroXI GAANNNNTTC 2 cut(s) 627, 885
MscI TGGCCA 1 cut(s) 319
MseI TTAA 2 cut(s) 732, 924
Msp20I TGGCCA 1 cut(s) 319
MspI CCGG 7 cut(s) 69, 147, 153, 160, 209, 740, 785
MspR9I CCNGG 4 cut(s) 161, 462, 566, 786
MvaI CCWGG 2 cut(s) 462, 566
MwoI GCNNNNNNNGC 2 cut(s) 149, 320
NaeI GCCGGC 2 cut(s) 148, 154
NarI GGCGCC 2 cut(s) 124, 150
NciI CCSGG 2 cut(s) 161, 786
NdeI CATATG 2 cut(s) 637, 649
NdeII GATC 5 cut(s) 135, 354, 570, 625, 681
NgoMIV GCCGGC 2 cut(s) 146, 152
NlaIII CATG 6 cut(s) 38, 88, 191, 458, 577, 712
NlaIV GGNNCC 5 cut(s) 67, 73, 125, 151, 213
NmuCI GTSAC 1 cut(s) 233
PagI TCATGA 1 cut(s) 454
PceI AGGCCT 1 cut(s) 224
PciSI GCTCTTC 1 cut(s) 342
PdiI GCCGGC 2 cut(s) 148, 154
PdmI GAANNNNTTC 2 cut(s) 627, 885
PfeI GAWTC 4 cut(s) 10, 99, 419, 644
PflFI GACNNNGTC 2 cut(s) 189, 239
PinAI ACCGGT 1 cut(s) 68
PkrI GCNGC 4 cut(s) 142, 145, 207, 556
PleI GAGTC 1 cut(s) 791
PluTI GGCGCC 2 cut(s) 127, 153
PpsI GAGTC 1 cut(s) 791
Psp6I CCWGG 2 cut(s) 460, 564
PspGI CCWGG 2 cut(s) 460, 564
PspN4I GGNNCC 5 cut(s) 67, 73, 125, 151, 213
PspPI GGNCC 4 cut(s) 4, 71, 162, 693
PstI CTGCAG 1 cut(s) 559
PsuI RGATCY 2 cut(s) 354, 625
PsyI GACNNNGTC 2 cut(s) 189, 239
RsaI GTAC 3 cut(s) 23, 33, 116
RsaNI GTAC 3 cut(s) 22, 32, 115
SapI GCTCTTC 1 cut(s) 342
SaqAI TTAA 2 cut(s) 732, 924
SatI GCNGC 4 cut(s) 141, 144, 206, 555
Sau3AI GATC 5 cut(s) 135, 354, 570, 625, 681
Sau96I GGNCC 4 cut(s) 4, 71, 162, 693
SchI GAGTC 1 cut(s) 791
ScrFI CCNGG 4 cut(s) 161, 462, 566, 786
SfaNI GCATC 1 cut(s) 484
SfcI CTRYAG 1 cut(s) 555
SfoI GGCGCC 2 cut(s) 125, 151
SgrAI CRCCGGYG 1 cut(s) 146
SinI GGWCC 3 cut(s) 4, 71, 693
SmlI CTYRAG 2 cut(s) 445, 876
SmoI CTYRAG 2 cut(s) 445, 876
Sse9I AATT 3 cut(s) 258, 726, 884
SseBI AGGCCT 1 cut(s) 224
SsiI CCGC 2 cut(s) 144, 206
SspDI GGCGCC 2 cut(s) 123, 149
SspI AATATT 1 cut(s) 517
SspMI CTAG 1 cut(s) 408
StuI AGGCCT 1 cut(s) 224
StyD4I CCNGG 4 cut(s) 159, 460, 564, 784
StyI CCWWGG 1 cut(s) 608
TaaI ACNGT 3 cut(s) 182, 233, 505
TaiI ACGT 1 cut(s) 54
TasI AATT 3 cut(s) 258, 726, 884
TatI WGTACW 1 cut(s) 21
TauI GCSGC 2 cut(s) 146, 208
TfiI GAWTC 4 cut(s) 10, 99, 419, 644
Tru1I TTAA 2 cut(s) 732, 924
Tru9I TTAA 2 cut(s) 732, 924
TscAI CASTG 1 cut(s) 814
TseFI GTSAC 1 cut(s) 233
TseI GCWGC 2 cut(s) 140, 554
Tsp45I GTSAC 1 cut(s) 233
TspDTI ATGAA 3 cut(s) 443, 471, 636
TspGWI ACGGA 1 cut(s) 111
TspRI CASTG 1 cut(s) 814
Tth111I GACNNNGTC 2 cut(s) 189, 239
VpaK11BI GGWCC 3 cut(s) 4, 71, 693
XmnI GAANNNNTTC 2 cut(s) 627, 885
XspI CTAG 1 cut(s) 408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.