Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr10

Orthogroup ID

OG0001460

Physical Location & Seq

Genomic Coordinates Reverse (-)

26718028 .. 26719592

Gene Structure 1565 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom10g25010.4

Sequence Viewer

CDS Protein Promoter (2k)

Length: 936 bp

ATGGAGCAAATCCAGCACAAGTACGTAACCGTACAAGGCCTGAAGCTCCACGTCGCCGACATCGGAACAGGCTCCAATGTGGTCGTGTTCCTGCACGGTTTCCCGGAAATTTGGTACTCGTGGCGGCACCAGATGGTTGCGGTAGCCAACGCCGGCTTCCGGGCCATCGCTCCCGACTACCGCGGATACGGACTATCCGACCCGCCTCCCCAATCGGAGAAGACCTCGTACCGTGACCTCGCCAGTGACATCCTTGGAATTCTTGATTCCCTTGCTATTCCCAAGGTTTTCCTAATTGCAAAAGATTTTGGAGTTGGGGTGGCGACATTATTTTCCCTTCTGCACGCGGAGAGGATCTTCGGCGTTGTAACAATGGGAGCGCCCATCATGCCACCAGGCCCTCGTAGAATCGATAAATCTCTCCAAGAAGGTCTCTACATTTCAAGATGGCAGGAACCTGGACGGGCAGAGGCTGATTTTGGGCGCCTTGATGCGAAAACAGTTGTTCGTAATGTTTACATCCTTTTCTCCAGAAGCGAAATACCAATAGCTGCAGAAGACCAGGAGATTATGGACCTCGTGGATCCATCTACGCCTCTTCCCCCTTGGTTCAGTGAGGAAGATCTTGCAGCATATGGAGCTTTGTATGAGAAATCAGGTTTCCGAACTGCGCTGCAAGTTCCGTACAGGGCAATTAGAGACGAGTCTGCTGTATCAAATCCAGTAGTTAAAGCTCCGGCACTTCTTATAAGGGGTGGCAAGGATTATTCGAACAAATTTCCAGGGATAGAGGATTACATAAGCAGTGGAAAGTTCAAAGAGTTTGCCCCCAATTTGGAGATTGTATTTCTTCCCGAAGGAACGCATTTTGTTCAAGAACAGTCACCTGACGAGGTCAATCAGCTTATCCTCACCTTCCTCGGAAAGCACATTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000287 GO:0001676 GO:0002532 GO:0002538 GO:0002539 GO:0003008 GO:0003013 GO:0003674 GO:0003824 GO:0004301 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005829 GO:0006082 GO:0006629 GO:0006631 GO:0006793 GO:0006796 GO:0006950 GO:0006952 GO:0006954 GO:0007600 GO:0008015 GO:0008150 GO:0008152 GO:0008217 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0016801 GO:0016803 GO:0019233 GO:0019752 GO:0030258 GO:0032501 GO:0032787 GO:0033559 GO:0042577 GO:0042578 GO:0042579 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043651 GO:0044237 GO:0044238 GO:0044255 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0045777 GO:0046839 GO:0046872 GO:0050877 GO:0050896 GO:0065007 GO:0065008 GO:0071704

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Abhydrolase_1 Abhydrolase_6

Protein Analysis

312

Amino Acids

34.56

Weight (kDa)

5.44

Isoelectric Point (pI)

45.03

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001460)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g05700 FvH4_3g04781 FvH4_3g04781 FvH4_3g04781 FvH4_3g04784 FvH4_3g04784 FvH4_3g04790 FvH4_3g04790 FvH4_3g04790
malus_domestica	MD05G1316900.v1.1 MD05G1317400.v1.1 MD10G1297200.v1.1
prunus_persica	Prupe.4G043200_v2.0.a1 Prupe.4G043200_v2.0.a1
pyrus_communis	pycom05g29400 pycom10g25010 pycom10g25060
rosa_chinensis	RchiOBHm_Chr5g0007821 RchiOBHm_Chr5g0007841 RchiOBHm_Chr5g0007871 RchiOBHm_Chr5g0007881
rosa_laevigata	RLG00000031499 RLG00000031500 RLG00000031501
rosa_multiflora	Rmu_co8472583.1_g000001 Rmu_sc0005098.1_g000007 Rmu_sc0005098.1_g000012
rosa_roxburghii	Rroxscaffold_1G00068550 Rroxscaffold_1G00068560 Rroxscaffold_1G00068580
rosa_rugosa	Rorug04G0430600 Rorug04G0430600 Rorug04G0430600 Rorug04G0430600 Rorug04G0430700 Rorug04G0430800
rosa_samantha	Rh5AG059200 Rh5AG059300 Rh5AG059600 Rh5AG059700 Rh5BG056200 Rh5BG056300 Rh5BG056400 Rh5BG056500 Rh5CG066000 Rh5CG066100 Rh5CG066400 Rh5DG055300 Rh5DG055500
rosa_wichuraiana	Rw5G006050 Rw5G006060 Rw5G006070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	749
AccB1I	GGYRCC	2 cut(s)	126, 483
AccII	CGCG	2 cut(s)	183, 347
AciI	CCGC	6 cut(s)	124, 140, 181, 183, 203, 347
AclWI	GGATC	3 cut(s)	362, 578, 591
AcsI	RAATTY	3 cut(s)	108, 258, 776
AcuI	CTGAAG	1 cut(s)	62
AcyI	GRCGYC	1 cut(s)	484
AfaI	GTAC	5 cut(s)	23, 33, 116, 230, 686
AfiI	CCNNNNNNNGG	2 cut(s)	159, 835
AgsI	TTSAA	3 cut(s)	444, 817, 875
AjiI	CACGTC	1 cut(s)	52
AjnI	CCWGG	4 cut(s)	394, 457, 561, 781
AjuI	GAANNNNNNNTTGG	2 cut(s)	140, 172
AluBI	AGCT	5 cut(s)	46, 551, 641, 734, 904
AluI	AGCT	5 cut(s)	46, 551, 641, 734, 904
Alw26I	GTCTC	2 cut(s)	437, 693
AlwI	GGATC	3 cut(s)	362, 578, 591
AlwNI	CAGNNNCTG	1 cut(s)	473
AoxI	GGCC	3 cut(s)	37, 162, 397
ApeKI	GCWGC	3 cut(s)	551, 629, 673
ApoI	RAATTY	3 cut(s)	108, 258, 776
AspLEI	GCGC	3 cut(s)	382, 486, 673
AspS9I	GGNCC	3 cut(s)	162, 398, 574
AsuC2I	CCSGG	2 cut(s)	104, 161
AsuHPI	GGTGA	2 cut(s)	876, 904
AsuII	TTCGAA	1 cut(s)	770
AvaII	GGWCC	1 cut(s)	574
BamHI	GGATCC	1 cut(s)	583
BanI	GGYRCC	2 cut(s)	126, 483
BarI	GAAGNNNNNNTAC	2 cut(s)	212, 244
BauI	CACGAG	2 cut(s)	118, 578
BbsI	GAAGAC	2 cut(s)	227, 564
BbvI	GCAGC	3 cut(s)	538, 641, 660
BccI	CCATC	5 cut(s)	127, 173, 392, 441, 595
BciT130I	CCWGG	4 cut(s)	396, 459, 563, 783
BciVI	GTATCC	1 cut(s)	179
BcnI	CCSGG	2 cut(s)	104, 161
BcoDI	GTCTC	2 cut(s)	437, 693
BfmI	CTRYAG	1 cut(s)	552
BfoI	RGCGCY	2 cut(s)	383, 487
BfuI	GTATCC	1 cut(s)	179
BglII	AGATCT	1 cut(s)	622
BisI	GCNGC	4 cut(s)	125, 552, 630, 674
BlsI	GCNGC	4 cut(s)	126, 553, 631, 675
Bme1390I	CCNGG	6 cut(s)	104, 161, 396, 459, 563, 783
Bme18I	GGWCC	1 cut(s)	574
BmgBI	CACGTC	1 cut(s)	52
BmgT120I	GGNCC	3 cut(s)	162, 398, 574
BmiI	GGNNCC	5 cut(s)	73, 128, 456, 485, 585
BmrFI	CCNGG	6 cut(s)	104, 161, 396, 459, 563, 783
BmsI	GCATC	1 cut(s)	481
BpiI	GAAGAC	2 cut(s)	227, 564
BplI	GAGNNNNNCTC	2 cut(s)	209, 241
BpmI	CTGGAG	1 cut(s)	514
Bpu14I	TTCGAA	1 cut(s)	770
BpuMI	CCSGG	2 cut(s)	104, 161
Bsa29I	ATCGAT	1 cut(s)	411
BsaAI	YACGTR	1 cut(s)	25
BsaHI	GRCGYC	1 cut(s)	484
BsaI	GGTCTC	1 cut(s)	437
BsaJI	CCNNGG	6 cut(s)	181, 253, 282, 605, 782, 919
BsaXI	ACNNNNNCTCC	2 cut(s)	303, 333
Bsc4I	CCNNNNNNNGG	2 cut(s)	159, 835
Bse118I	RCCGGY	1 cut(s)	152
Bse1I	ACTGG	2 cut(s)	243, 722
BseBI	CCWGG	4 cut(s)	396, 459, 563, 783
BseCI	ATCGAT	1 cut(s)	411
BseDI	CCNNGG	6 cut(s)	181, 253, 282, 605, 782, 919
BseGI	GGATG	2 cut(s)	249, 519
BseLI	CCNNNNNNNGG	2 cut(s)	159, 835
BseNI	ACTGG	2 cut(s)	243, 722
BseXI	GCAGC	3 cut(s)	538, 641, 660
BsgI	GTGCAG	2 cut(s)	77, 326
Bsh1236I	CGCG	2 cut(s)	183, 347
BshFI	GGCC	3 cut(s)	39, 164, 399
BshNI	GGYRCC	2 cut(s)	126, 483
BshVI	ATCGAT	1 cut(s)	411
BsiSI	CCGG	4 cut(s)	104, 153, 160, 737
BslI	CCNNNNNNNGG	2 cut(s)	159, 835
BsmAI	GTCTC	2 cut(s)	437, 693
BsmBI	CGTCTC	1 cut(s)	693
BsnI	GGCC	3 cut(s)	39, 164, 399
Bso31I	GGTCTC	1 cut(s)	437
Bsp119I	TTCGAA	1 cut(s)	770
Bsp143I	GATC	3 cut(s)	354, 583, 622
BspACI	CCGC	6 cut(s)	124, 140, 181, 183, 203, 347
BspANI	GGCC	3 cut(s)	39, 164, 399
BspDI	ATCGAT	1 cut(s)	411
BspFNI	CGCG	2 cut(s)	183, 347
BspLI	GGNNCC	5 cut(s)	73, 128, 456, 485, 585
BspMAI	CTGCAG	1 cut(s)	556
BspPI	GGATC	3 cut(s)	362, 578, 591
BspT104I	TTCGAA	1 cut(s)	770
BspT107I	GGYRCC	2 cut(s)	126, 483
BspTNI	GGTCTC	1 cut(s)	437
BsrFI	RCCGGY	1 cut(s)	152
BsrI	ACTGG	2 cut(s)	243, 722
BssAI	RCCGGY	1 cut(s)	152
BssECI	CCNNGG	6 cut(s)	181, 253, 282, 605, 782, 919
BssMI	GATC	3 cut(s)	354, 583, 622
BssNI	GRCGYC	1 cut(s)	484
BssSI	CACGAG	2 cut(s)	118, 578
BssT1I	CCWWGG	3 cut(s)	253, 282, 605
Bst2BI	CACGAG	2 cut(s)	118, 578
Bst2UI	CCWGG	4 cut(s)	396, 459, 563, 783
Bst4CI	ACNGT	5 cut(s)	31, 98, 233, 502, 882
Bst6I	CTCTTC	1 cut(s)	603
BstACI	GRCGYC	1 cut(s)	484
BstBAI	YACGTR	1 cut(s)	25
BstBI	TTCGAA	1 cut(s)	770
BstC8I	GCNNGC	2 cut(s)	154, 345
BstDSI	CCRYGG	1 cut(s)	181
BstF5I	GGATG	2 cut(s)	249, 519
BstFNI	CGCG	2 cut(s)	183, 347
BstH2I	RGCGCY	2 cut(s)	383, 487
BstHHI	GCGC	3 cut(s)	382, 486, 673
BstKTI	GATC	3 cut(s)	357, 586, 625
BstMAI	GTCTC	2 cut(s)	437, 693
BstMBI	GATC	3 cut(s)	354, 583, 622
BstMWI	GCNNNNNNNGC	3 cut(s)	13, 388, 638
BstNI	CCWGG	4 cut(s)	396, 459, 563, 783
BstSCI	CCNGG	6 cut(s)	102, 159, 394, 457, 561, 781
BstSFI	CTRYAG	1 cut(s)	552
BstSNI	TACGTA	1 cut(s)	25
BstUI	CGCG	2 cut(s)	183, 347
BstV1I	GCAGC	3 cut(s)	538, 641, 660
BstV2I	GAAGAC	2 cut(s)	227, 564
BstX2I	RGATCY	3 cut(s)	354, 583, 622
BstYI	RGATCY	3 cut(s)	354, 583, 622
Bsu15I	ATCGAT	1 cut(s)	411
BsuI	GTATCC	1 cut(s)	179
BsuRI	GGCC	3 cut(s)	39, 164, 399
BsuTUI	ATCGAT	1 cut(s)	411
BtgI	CCRYGG	1 cut(s)	181
BtgZI	GCGATG	1 cut(s)	151
BtrI	CACGTC	1 cut(s)	52
BtsCI	GGATG	2 cut(s)	249, 519
BtsI	GCAGTG	1 cut(s)	811
BtsIMutI	CAGTG	3 cut(s)	250, 619, 811
Cac8I	GCNNGC	2 cut(s)	154, 345
CaiI	CAGNNNCTG	1 cut(s)	473
CfoI	GCGC	3 cut(s)	382, 486, 673
Cfr10I	RCCGGY	1 cut(s)	152
Cfr13I	GGNCC	3 cut(s)	162, 398, 574
Cfr42I	CCGCGG	1 cut(s)	184
ClaI	ATCGAT	1 cut(s)	411
Csp6I	GTAC	5 cut(s)	22, 32, 115, 229, 685
CviAII	CATG	1 cut(s)	388
CviQI	GTAC	5 cut(s)	22, 32, 115, 229, 685
DinI	GGCGCC	1 cut(s)	485
DpnI	GATC	3 cut(s)	356, 585, 624
DpnII	GATC	3 cut(s)	354, 583, 622
Eam1104I	CTCTTC	1 cut(s)	603
EarI	CTCTTC	1 cut(s)	603
Eco105I	TACGTA	1 cut(s)	25
Eco130I	CCWWGG	3 cut(s)	253, 282, 605
Eco147I	AGGCCT	1 cut(s)	39
Eco31I	GGTCTC	1 cut(s)	437
Eco47I	GGWCC	1 cut(s)	574
Eco57I	CTGAAG	1 cut(s)	62
EcoO109I	RGGNCCY	1 cut(s)	398
EcoRI	GAATTC	1 cut(s)	258
EcoRII	CCWGG	4 cut(s)	394, 457, 561, 781
EcoT14I	CCWWGG	3 cut(s)	253, 282, 605
EgeI	GGCGCC	1 cut(s)	485
EheI	GGCGCC	1 cut(s)	485
ErhI	CCWWGG	3 cut(s)	253, 282, 605
Esp3I	CGTCTC	1 cut(s)	693
FaeI	CATG	1 cut(s)	391
FaiI	YATR	7 cut(s)	389, 572, 634, 636, 648, 749, 800
FatI	CATG	1 cut(s)	387
FauI	CCCGC	1 cut(s)	210
FauNDI	CATATG	1 cut(s)	634
Fnu4HI	GCNGC	4 cut(s)	125, 552, 630, 674
FokI	GGATG	2 cut(s)	236, 506
Fsp4HI	GCNGC	4 cut(s)	125, 552, 630, 674
GlaI	GCGC	3 cut(s)	381, 485, 672
GluI	GCNGC	4 cut(s)	125, 552, 630, 674
GsuI	CTGGAG	1 cut(s)	514
HaeII	RGCGCY	2 cut(s)	383, 487
HaeIII	GGCC	3 cut(s)	39, 164, 399
HapII	CCGG	4 cut(s)	104, 153, 160, 737
HhaI	GCGC	3 cut(s)	382, 486, 673
Hin1I	GRCGYC	1 cut(s)	484
Hin1II	CATG	1 cut(s)	391
Hin6I	GCGC	3 cut(s)	380, 484, 671
HinP1I	GCGC	3 cut(s)	380, 484, 671
HinfI	GANTC	3 cut(s)	266, 408, 704
HpaII	CCGG	4 cut(s)	104, 153, 160, 737
HphI	GGTGA	2 cut(s)	876, 904
Hpy166II	GTNNAC	1 cut(s)	517
Hpy188I	TCNGA	5 cut(s)	65, 199, 217, 665, 923
Hpy188III	TCNNGA	6 cut(s)	173, 263, 444, 531, 854, 875
Hpy8I	GTNNAC	1 cut(s)	517
Hpy99I	CGWCG	1 cut(s)	56
HpyAV	CCTTC	4 cut(s)	347, 422, 851, 925
HpyCH4III	ACNGT	5 cut(s)	31, 98, 233, 502, 882
HpyCH4IV	ACGT	2 cut(s)	24, 51
HpyCH4V	TGCA	6 cut(s)	94, 299, 343, 554, 629, 676
HpyF10VI	GCNNNNNNNGC	3 cut(s)	13, 388, 638
HpySE526I	ACGT	2 cut(s)	24, 51
Hsp92I	GRCGYC	1 cut(s)	484
Hsp92II	CATG	1 cut(s)	391
HspAI	GCGC	3 cut(s)	380, 484, 671
KasI	GGCGCC	1 cut(s)	483
KroI	GCCGGC	1 cut(s)	152
KroNI	GCCGGC	1 cut(s)	154
KspI	CCGCGG	1 cut(s)	184
Kzo9I	GATC	3 cut(s)	354, 583, 622
LmnI	GCTCC	7 cut(s)	4, 51, 77, 175, 377, 638, 739
Lsp1109I	GCAGC	3 cut(s)	538, 641, 660
LweI	GCATC	1 cut(s)	481
MaeII	ACGT	2 cut(s)	24, 51
MaeIII	GTNAC	5 cut(s)	25, 233, 245, 367, 882
MalI	GATC	3 cut(s)	356, 585, 624
MboI	GATC	3 cut(s)	354, 583, 622
MboII	GAAGA	6 cut(s)	232, 349, 569, 590, 632, 842
MflI	RGATCY	3 cut(s)	354, 583, 622
MluCI	AATT	6 cut(s)	108, 258, 294, 693, 776, 832
Mly113I	GGCGCC	1 cut(s)	484
MlyI	GAGTC	1 cut(s)	713
MmeI	TCCRAC	1 cut(s)	222
MroNI	GCCGGC	1 cut(s)	152
MseI	TTAA	1 cut(s)	729
MspA1I	CMGCKG	1 cut(s)	183
MspI	CCGG	4 cut(s)	104, 153, 160, 737
MspR9I	CCNGG	6 cut(s)	104, 161, 396, 459, 563, 783
MvaI	CCWGG	4 cut(s)	396, 459, 563, 783
MvnI	CGCG	2 cut(s)	183, 347
MwoI	GCNNNNNNNGC	3 cut(s)	13, 388, 638
NaeI	GCCGGC	1 cut(s)	154
NarI	GGCGCC	1 cut(s)	484
NciI	CCSGG	2 cut(s)	104, 161
NdeI	CATATG	1 cut(s)	634
NdeII	GATC	3 cut(s)	354, 583, 622
NgoMIV	GCCGGC	1 cut(s)	152
NlaIII	CATG	1 cut(s)	391
NlaIV	GGNNCC	5 cut(s)	73, 128, 456, 485, 585
NmuCI	GTSAC	3 cut(s)	233, 245, 882
NspV	TTCGAA	1 cut(s)	770
PceI	AGGCCT	1 cut(s)	39
PcsI	WCGNNNNNNNCGW	1 cut(s)	195
PdiI	GCCGGC	1 cut(s)	154
PfeI	GAWTC	2 cut(s)	266, 408
PflFI	GACNNNGTC	1 cut(s)	893
PfoI	TCCNGGA	1 cut(s)	102
PkrI	GCNGC	4 cut(s)	126, 553, 631, 675
PleI	GAGTC	1 cut(s)	712
PluTI	GGCGCC	1 cut(s)	487
PpsI	GAGTC	1 cut(s)	712
Ppu21I	YACGTR	1 cut(s)	25
PsiI	TTATAA	1 cut(s)	749
Psp6I	CCWGG	4 cut(s)	394, 457, 561, 781
PspGI	CCWGG	4 cut(s)	394, 457, 561, 781
PspN4I	GGNNCC	5 cut(s)	73, 128, 456, 485, 585
PspPI	GGNCC	3 cut(s)	162, 398, 574
PstI	CTGCAG	1 cut(s)	556
PstNI	CAGNNNCTG	1 cut(s)	473
PsuI	RGATCY	3 cut(s)	354, 583, 622
PsyI	GACNNNGTC	1 cut(s)	893
RsaI	GTAC	5 cut(s)	23, 33, 116, 230, 686
RsaNI	GTAC	5 cut(s)	22, 32, 115, 229, 685
SacII	CCGCGG	1 cut(s)	184
SaqAI	TTAA	1 cut(s)	729
SatI	GCNGC	4 cut(s)	125, 552, 630, 674
Sau3AI	GATC	3 cut(s)	354, 583, 622
Sau96I	GGNCC	3 cut(s)	162, 398, 574
SchI	GAGTC	1 cut(s)	713
ScrFI	CCNGG	6 cut(s)	104, 161, 396, 459, 563, 783
SfaNI	GCATC	1 cut(s)	481
SfcI	CTRYAG	1 cut(s)	552
SfoI	GGCGCC	1 cut(s)	485
Sfr303I	CCGCGG	1 cut(s)	184
SfuI	TTCGAA	1 cut(s)	770
SgrBI	CCGCGG	1 cut(s)	184
SinI	GGWCC	1 cut(s)	574
SnaBI	TACGTA	1 cut(s)	25
Sse9I	AATT	6 cut(s)	108, 258, 294, 693, 776, 832
SseBI	AGGCCT	1 cut(s)	39
SsiI	CCGC	6 cut(s)	124, 140, 181, 183, 203, 347
SspDI	GGCGCC	1 cut(s)	483
StuI	AGGCCT	1 cut(s)	39
StyD4I	CCNGG	6 cut(s)	102, 159, 394, 457, 561, 781
StyI	CCWWGG	3 cut(s)	253, 282, 605
TaaI	ACNGT	5 cut(s)	31, 98, 233, 502, 882
TaiI	ACGT	2 cut(s)	27, 54
TaqI	TCGA	2 cut(s)	411, 770
TasI	AATT	6 cut(s)	108, 258, 294, 693, 776, 832
TauI	GCSGC	1 cut(s)	127
TfiI	GAWTC	2 cut(s)	266, 408
Tru1I	TTAA	1 cut(s)	729
Tru9I	TTAA	1 cut(s)	729
TscAI	CASTG	3 cut(s)	250, 619, 811
TseFI	GTSAC	3 cut(s)	233, 245, 882
TseI	GCWGC	3 cut(s)	551, 629, 673
Tsp45I	GTSAC	3 cut(s)	233, 245, 882
TspGWI	ACGGA	2 cut(s)	204, 672
TspRI	CASTG	3 cut(s)	250, 619, 811
Tth111I	GACNNNGTC	1 cut(s)	893
VpaK11BI	GGWCC	1 cut(s)	574
XapI	RAATTY	3 cut(s)	108, 258, 776

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.