Rw5G006050

Bifunctional epoxide hydrolase 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0001460
Physical Location & Seq
Reverse (-)
6268966 .. 6270586
1621 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G006050.1

Sequence Viewer

Length: 939 bp
ATGGATCAAATCCAGCACAACTTCATAACAGTGCAAGGCCTCAAGCTCCACGTAGCCGACATCGGAACAGGTCCCAATGTGGTCTTGTTCCTGCACGGATTCCCAGAAATATGGTACACGTGGCGCCACCAGATGATCGCTGCCGCCAACGCCGGTTTCCGGGCCATCGCGCCTGACTACCGTGGACACGGTCTTTCCGACCAGCCTCCCGAACCCGACAAGGCCTCCTACCGTGACCTCGTCAACGACATCCTTGCTATTCTTGAAGCTCTTGCTATTACCAAGGTTTTCGTTGTCGCAAAAGATTTTGGAGCTTGGCCAGCGTACATACTTGCCCTTCTTTACCCGGATAGGATCTTGGGAGTTGTAACATTGGGGGTGCCATACATACCACCTTCAAGTCGTCCCAATTTCACCAAATCCCTCCCTGAAGGTTTCTACATTTCAAGATTTCATGAACCAGGCAGGGCTGAGGCTGATTTTGGGCGCTTTGATGCGACAACAGTTTTTCGGAATATTTACATCCTTTTCTCCAGAAGTGAAATCCCAATAGCTGCAGAAAACCAGGAGATCATGGACTTGGTTGACCCATCTACGCCTCTTCCCCCATGGTTCTCTGAGGAAGATCTTAAAGCATATGGAGCTGCATATGAGAGAACAGGTTTCCAGACTGCATTGCAGATCACATATAGGTCCAAGTCAGACGACATGGGCATAACAGAACTAATTGTTAAAGTTCCGGTGCTTGTTATAATGGGACGGAAGGATTACTTTAACAAGTTTCCGGGGATAGAGGACTCTATAACCAGTGGAAAGGTGAACGAGTTTACGTCTGATTTGGAGCTTGTATATATGCCTGAAGGAACACATTTTGCTCAAGAACAAGCTCCTGACCAGTTCAATCAGCTAATGCTCACCTTCCTTAAAAAGCACATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000287 GO:0001676 GO:0002532 GO:0002538 GO:0002539 GO:0003008 GO:0003013 GO:0003018 GO:0003674 GO:0003824 GO:0004301 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005782 GO:0005829 GO:0006082 GO:0006605 GO:0006625 GO:0006629 GO:0006631 GO:0006633 GO:0006690 GO:0006725 GO:0006793 GO:0006796 GO:0006805 GO:0006810 GO:0006873 GO:0006874 GO:0006875 GO:0006886 GO:0006950 GO:0006952 GO:0006954 GO:0006996 GO:0007031 GO:0007600 GO:0008015 GO:0008104 GO:0008150 GO:0008152 GO:0008217 GO:0008610 GO:0009056 GO:0009058 GO:0009410 GO:0009636 GO:0009810 GO:0009893 GO:0009987 GO:0010468 GO:0010604 GO:0010628 GO:0015031 GO:0015643 GO:0015833 GO:0016043 GO:0016053 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0016801 GO:0016803 GO:0017144 GO:0018904 GO:0019216 GO:0019218 GO:0019222 GO:0019233 GO:0019369 GO:0019373 GO:0019439 GO:0019725 GO:0019752 GO:0030003 GO:0030258 GO:0031907 GO:0031974 GO:0032501 GO:0032787 GO:0033036 GO:0033365 GO:0033559 GO:0034613 GO:0035150 GO:0035296 GO:0042221 GO:0042577 GO:0042578 GO:0042579 GO:0042592 GO:0042632 GO:0042759 GO:0042802 GO:0042803 GO:0042886 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043574 GO:0043651 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0045777 GO:0046272 GO:0046394 GO:0046483 GO:0046839 GO:0046872 GO:0046907 GO:0046983 GO:0048518 GO:0048878 GO:0050789 GO:0050801 GO:0050877 GO:0050880 GO:0050896 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051716 GO:0055065 GO:0055074 GO:0055080 GO:0055082 GO:0055088 GO:0055092 GO:0060255 GO:0062012 GO:0065007 GO:0065008 GO:0070013 GO:0070727 GO:0070887 GO:0071466 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072330 GO:0072503 GO:0072507 GO:0072593 GO:0072594 GO:0072662 GO:0072663 GO:0080090 GO:0090066 GO:0090181 GO:0097176 GO:0097746 GO:0097755 GO:0098771 GO:1900673 GO:1901360 GO:1901361 GO:1901568 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

312

Amino Acids

35.16

Weight (kDa)

5.19

Isoelectric Point (pI)

39.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 26 - 258 9.4e-25 alpha/beta hydrolase fold
Hydrolase_4 PF12146 27 - 126 2e-08 Serine aminopeptidase, S33
Abhydrolase_6 PF12697 28 - 302 9.3e-16 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 752
AccB1I GGYRCC 2 cut(s) 123, 379
AccII CGCG 1 cut(s) 170
AciI CCGC 1 cut(s) 144
AclWI GGATC 2 cut(s) 12, 362
AcoI YGGCCR 1 cut(s) 317
AcuI CTGAAG 2 cut(s) 450, 879
AcvI CACGTG 1 cut(s) 120
AcyI GRCGYC 1 cut(s) 124
AfaI GTAC 2 cut(s) 116, 326
AfiI CCNNNNNNNGG 1 cut(s) 159
AflIII ACRYGT 1 cut(s) 117
AgsI TTSAA 4 cut(s) 266, 399, 447, 901
AjnI CCWGG 2 cut(s) 460, 564
AjuI GAANNNNNNNTTGG 2 cut(s) 140, 172
AluBI AGCT 8 cut(s) 46, 269, 314, 554, 644, 844, 887, 907
AluI AGCT 8 cut(s) 46, 269, 314, 554, 644, 844, 887, 907
AlwI GGATC 2 cut(s) 12, 362
AoxI GGCC 4 cut(s) 37, 162, 222, 317
ApeKI GCWGC 3 cut(s) 140, 554, 644
AspLEI GCGC 3 cut(s) 126, 172, 489
AspS9I GGNCC 3 cut(s) 71, 162, 693
AsuC2I CCSGG 3 cut(s) 161, 347, 786
AsuHPI GGTGA 3 cut(s) 406, 829, 907
AvaII GGWCC 2 cut(s) 71, 693
BalI TGGCCA 1 cut(s) 319
BanI GGYRCC 2 cut(s) 123, 379
BbrPI CACGTG 1 cut(s) 120
BbvCI CCTCAGC 1 cut(s) 471
BbvI GCAGC 3 cut(s) 127, 541, 631
BccI CCATC 2 cut(s) 173, 598
BcgI CGANNNNNNTGC 2 cut(s) 236, 270
BciT130I CCWGG 2 cut(s) 462, 566
BcnI CCSGG 3 cut(s) 161, 347, 786
BfmI CTRYAG 1 cut(s) 555
BfoI RGCGCY 2 cut(s) 127, 490
BglII AGATCT 1 cut(s) 625
BisI GCNGC 4 cut(s) 141, 144, 555, 645
BlsI GCNGC 4 cut(s) 142, 145, 556, 646
Bme1390I CCNGG 5 cut(s) 161, 347, 462, 566, 786
Bme18I GGWCC 2 cut(s) 71, 693
BmgT120I GGNCC 3 cut(s) 71, 162, 693
BmiI GGNNCC 3 cut(s) 73, 125, 381
BmrFI CCNGG 5 cut(s) 161, 347, 462, 566, 786
BmsI GCATC 1 cut(s) 484
BpmI CTGGAG 1 cut(s) 517
Bpu10I CCTNAGC 1 cut(s) 471
BpuEI CTTGAG 2 cut(s) 26, 861
BpuMI CCSGG 3 cut(s) 161, 347, 786
BsaAI YACGTR 2 cut(s) 52, 120
BsaHI GRCGYC 1 cut(s) 124
BsaJI CCNNGG 4 cut(s) 181, 282, 608, 785
BsaWI WCCGGW 1 cut(s) 739
BsaXI ACNNNNNCTCC 2 cut(s) 209, 239
Bsc4I CCNNNNNNNGG 1 cut(s) 159
Bse118I RCCGGY 1 cut(s) 152
Bse1I ACTGG 2 cut(s) 807, 895
Bse3DI GCAATG 1 cut(s) 674
BseBI CCWGG 2 cut(s) 462, 566
BseDI CCNNGG 4 cut(s) 181, 282, 608, 785
BseGI GGATG 2 cut(s) 249, 522
BseLI CCNNNNNNNGG 1 cut(s) 159
BseMI GCAATG 1 cut(s) 674
BseMII CTCAG 2 cut(s) 462, 609
BseNI ACTGG 2 cut(s) 807, 895
BseXI GCAGC 3 cut(s) 127, 541, 631
BsgI GTGCAG 1 cut(s) 77
Bsh1236I CGCG 1 cut(s) 170
BshFI GGCC 4 cut(s) 39, 164, 224, 319
BshNI GGYRCC 2 cut(s) 123, 379
BsiSI CCGG 5 cut(s) 153, 160, 347, 740, 785
BslFI GGGAC 3 cut(s) 57, 390, 771
BslI CCNNNNNNNGG 1 cut(s) 159
BsmFI GGGAC 3 cut(s) 57, 390, 771
BsnI GGCC 4 cut(s) 39, 164, 224, 319
Bsp143I GATC 6 cut(s) 4, 135, 354, 570, 625, 681
Bsp19I CCATGG 1 cut(s) 608
BspACI CCGC 1 cut(s) 144
BspANI GGCC 4 cut(s) 39, 164, 224, 319
BspCNI CTCAG 2 cut(s) 463, 610
BspFNI CGCG 1 cut(s) 170
BspHI TCATGA 1 cut(s) 454
BspLI GGNNCC 3 cut(s) 73, 125, 381
BspMAI CTGCAG 1 cut(s) 559
BspPI GGATC 2 cut(s) 12, 362
BspT107I GGYRCC 2 cut(s) 123, 379
BsrDI GCAATG 1 cut(s) 674
BsrFI RCCGGY 1 cut(s) 152
BsrI ACTGG 2 cut(s) 807, 895
BssAI RCCGGY 1 cut(s) 152
BssECI CCNNGG 4 cut(s) 181, 282, 608, 785
BssMI GATC 6 cut(s) 4, 135, 354, 570, 625, 681
BssNI GRCGYC 1 cut(s) 124
BssT1I CCWWGG 2 cut(s) 282, 608
Bst2UI CCWGG 2 cut(s) 462, 566
Bst4CI ACNGT 5 cut(s) 31, 182, 191, 233, 505
Bst6I CTCTTC 1 cut(s) 606
BstACI GRCGYC 1 cut(s) 124
BstBAI YACGTR 2 cut(s) 52, 120
BstC8I GCNNGC 1 cut(s) 321
BstDEI CTNAG 2 cut(s) 471, 618
BstDSI CCRYGG 2 cut(s) 181, 608
BstF5I GGATG 2 cut(s) 249, 522
BstFNI CGCG 1 cut(s) 170
BstH2I RGCGCY 2 cut(s) 127, 490
BstHHI GCGC 3 cut(s) 126, 172, 489
BstKTI GATC 6 cut(s) 7, 138, 357, 573, 628, 684
BstMBI GATC 6 cut(s) 4, 135, 354, 570, 625, 681
BstMWI GCNNNNNNNGC 3 cut(s) 149, 320, 641
BstNI CCWGG 2 cut(s) 462, 566
BstSCI CCNGG 5 cut(s) 159, 345, 460, 564, 784
BstSFI CTRYAG 1 cut(s) 555
BstUI CGCG 1 cut(s) 170
BstV1I GCAGC 3 cut(s) 127, 541, 631
BstX2I RGATCY 2 cut(s) 354, 625
BstXI CCANNNNNNTGG 1 cut(s) 111
BstYI RGATCY 2 cut(s) 354, 625
BsuRI GGCC 4 cut(s) 39, 164, 224, 319
BtgI CCRYGG 2 cut(s) 181, 608
BtgZI GCGATG 1 cut(s) 151
BtsCI GGATG 2 cut(s) 249, 522
BtsIMutI CAGTG 2 cut(s) 36, 814
Cac8I GCNNGC 1 cut(s) 321
CciI TCATGA 1 cut(s) 454
CfoI GCGC 3 cut(s) 126, 172, 489
Cfr10I RCCGGY 1 cut(s) 152
Cfr13I GGNCC 3 cut(s) 71, 162, 693
Csp6I GTAC 2 cut(s) 115, 325
CviAII CATG 4 cut(s) 455, 574, 609, 709
CviQI GTAC 2 cut(s) 115, 325
DdeI CTNAG 2 cut(s) 471, 618
DinI GGCGCC 1 cut(s) 125
DpnI GATC 6 cut(s) 6, 137, 356, 572, 627, 683
DpnII GATC 6 cut(s) 4, 135, 354, 570, 625, 681
EaeI YGGCCR 1 cut(s) 317
Eam1104I CTCTTC 1 cut(s) 606
EarI CTCTTC 1 cut(s) 606
Eco130I CCWWGG 2 cut(s) 282, 608
Eco147I AGGCCT 2 cut(s) 39, 224
Eco47I GGWCC 2 cut(s) 71, 693
Eco57I CTGAAG 2 cut(s) 450, 879
Eco72I CACGTG 1 cut(s) 120
EcoO109I RGGNCCY 1 cut(s) 71
EcoRII CCWGG 2 cut(s) 460, 564
EcoT14I CCWWGG 2 cut(s) 282, 608
EgeI GGCGCC 1 cut(s) 125
EheI GGCGCC 1 cut(s) 125
ErhI CCWWGG 2 cut(s) 282, 608
FaeI CATG 4 cut(s) 458, 577, 612, 712
FalI AAGNNNNNCTT 2 cut(s) 755, 787
FaqI GGGAC 3 cut(s) 57, 390, 771
FatI CATG 4 cut(s) 454, 573, 608, 708
FauNDI CATATG 2 cut(s) 637, 649
Fnu4HI GCNGC 4 cut(s) 141, 144, 555, 645
FokI GGATG 2 cut(s) 236, 509
Fsp4HI GCNGC 4 cut(s) 141, 144, 555, 645
GlaI GCGC 3 cut(s) 125, 171, 488
GluI GCNGC 4 cut(s) 141, 144, 555, 645
GsuI CTGGAG 1 cut(s) 517
HaeII RGCGCY 2 cut(s) 127, 490
HaeIII GGCC 4 cut(s) 39, 164, 224, 319
HapII CCGG 5 cut(s) 153, 160, 347, 740, 785
HhaI GCGC 3 cut(s) 126, 172, 489
Hin1I GRCGYC 1 cut(s) 124
Hin1II CATG 4 cut(s) 458, 577, 612, 712
Hin6I GCGC 3 cut(s) 124, 170, 487
HinP1I GCGC 3 cut(s) 124, 170, 487
HincII GTYRAC 2 cut(s) 244, 586
HindII GTYRAC 2 cut(s) 244, 586
HinfI GANTC 2 cut(s) 99, 797
HpaII CCGG 5 cut(s) 153, 160, 347, 740, 785
HphI GGTGA 3 cut(s) 406, 829, 907
Hpy166II GTNNAC 6 cut(s) 117, 185, 244, 586, 820, 828
Hpy188I TCNGA 7 cut(s) 65, 199, 513, 619, 703, 835, 938
Hpy188III TCNNGA 8 cut(s) 209, 263, 447, 455, 534, 667, 878, 890
Hpy8I GTNNAC 6 cut(s) 117, 185, 244, 586, 820, 828
HpyAV CCTTC 6 cut(s) 347, 405, 425, 757, 854, 928
HpyCH4III ACNGT 5 cut(s) 31, 182, 191, 233, 505
HpyCH4IV ACGT 3 cut(s) 51, 119, 830
HpyCH4V TGCA 6 cut(s) 34, 94, 557, 647, 674, 679
HpyF10VI GCNNNNNNNGC 3 cut(s) 149, 320, 641
HpyF3I CTNAG 2 cut(s) 471, 618
HpySE526I ACGT 3 cut(s) 51, 119, 830
Hsp92I GRCGYC 1 cut(s) 124
Hsp92II CATG 4 cut(s) 458, 577, 612, 712
HspAI GCGC 3 cut(s) 124, 170, 487
KasI GGCGCC 1 cut(s) 123
Kzo9I GATC 6 cut(s) 4, 135, 354, 570, 625, 681
LmnI GCTCC 5 cut(s) 51, 311, 641, 841, 892
Lsp1109I GCAGC 3 cut(s) 127, 541, 631
LweI GCATC 1 cut(s) 484
MaeII ACGT 3 cut(s) 51, 119, 830
MaeIII GTNAC 2 cut(s) 233, 367
MalI GATC 6 cut(s) 6, 137, 356, 572, 627, 683
MboI GATC 6 cut(s) 4, 135, 354, 570, 625, 681
MboII GAAGA 2 cut(s) 593, 635
MflI RGATCY 2 cut(s) 354, 625
MlsI TGGCCA 1 cut(s) 319
MluCI AATT 2 cut(s) 409, 726
MluNI TGGCCA 1 cut(s) 319
Mly113I GGCGCC 1 cut(s) 124
MlyI GAGTC 1 cut(s) 791
MmeI TCCRAC 1 cut(s) 222
MnlI CCTC 9 cut(s) 50, 216, 235, 248, 434, 466, 609, 613, 787
Mox20I TGGCCA 1 cut(s) 319
MscI TGGCCA 1 cut(s) 319
MseI TTAA 4 cut(s) 630, 732, 774, 924
MslI CAYNNNNRTG 1 cut(s) 29
Msp20I TGGCCA 1 cut(s) 319
MspI CCGG 5 cut(s) 153, 160, 347, 740, 785
MspR9I CCNGG 5 cut(s) 161, 347, 462, 566, 786
MvaI CCWGG 2 cut(s) 462, 566
MvnI CGCG 1 cut(s) 170
MwoI GCNNNNNNNGC 3 cut(s) 149, 320, 641
NarI GGCGCC 1 cut(s) 124
NciI CCSGG 3 cut(s) 161, 347, 786
NcoI CCATGG 1 cut(s) 608
NdeI CATATG 2 cut(s) 637, 649
NdeII GATC 6 cut(s) 4, 135, 354, 570, 625, 681
NlaIII CATG 4 cut(s) 458, 577, 612, 712
NlaIV GGNNCC 3 cut(s) 73, 125, 381
NmuCI GTSAC 1 cut(s) 233
PagI TCATGA 1 cut(s) 454
PceI AGGCCT 2 cut(s) 39, 224
PcsI WCGNNNNNNNCGW 1 cut(s) 195
PfeI GAWTC 1 cut(s) 99
PflFI GACNNNGTC 2 cut(s) 189, 239
PkrI GCNGC 4 cut(s) 142, 145, 556, 646
PleI GAGTC 1 cut(s) 791
PluTI GGCGCC 1 cut(s) 127
PmaCI CACGTG 1 cut(s) 120
PmlI CACGTG 1 cut(s) 120
PpsI GAGTC 1 cut(s) 791
Ppu21I YACGTR 2 cut(s) 52, 120
PpuMI RGGWCCY 1 cut(s) 71
PsiI TTATAA 1 cut(s) 752
Psp5II RGGWCCY 1 cut(s) 71
Psp6I CCWGG 2 cut(s) 460, 564
PspCI CACGTG 1 cut(s) 120
PspGI CCWGG 2 cut(s) 460, 564
PspN4I GGNNCC 3 cut(s) 73, 125, 381
PspPI GGNCC 3 cut(s) 71, 162, 693
PspPPI RGGWCCY 1 cut(s) 71
PstI CTGCAG 1 cut(s) 559
PsuI RGATCY 2 cut(s) 354, 625
PsyI GACNNNGTC 2 cut(s) 189, 239
RsaI GTAC 2 cut(s) 116, 326
RsaNI GTAC 2 cut(s) 115, 325
RseI CAYNNNNRTG 1 cut(s) 29
SaqAI TTAA 4 cut(s) 630, 732, 774, 924
SatI GCNGC 4 cut(s) 141, 144, 555, 645
Sau3AI GATC 6 cut(s) 4, 135, 354, 570, 625, 681
Sau96I GGNCC 3 cut(s) 71, 162, 693
SchI GAGTC 1 cut(s) 791
ScrFI CCNGG 5 cut(s) 161, 347, 462, 566, 786
SfaNI GCATC 1 cut(s) 484
SfcI CTRYAG 1 cut(s) 555
SfoI GGCGCC 1 cut(s) 125
SinI GGWCC 2 cut(s) 71, 693
SmiMI CAYNNNNRTG 1 cut(s) 29
SmlI CTYRAG 2 cut(s) 41, 876
SmoI CTYRAG 2 cut(s) 41, 876
Sse9I AATT 2 cut(s) 409, 726
SseBI AGGCCT 2 cut(s) 39, 224
SsiI CCGC 1 cut(s) 144
SspDI GGCGCC 1 cut(s) 123
SspI AATATT 1 cut(s) 517
StuI AGGCCT 2 cut(s) 39, 224
StyD4I CCNGG 5 cut(s) 159, 345, 460, 564, 784
StyI CCWWGG 2 cut(s) 282, 608
TaaI ACNGT 5 cut(s) 31, 182, 191, 233, 505
TaiI ACGT 3 cut(s) 54, 122, 833
TasI AATT 2 cut(s) 409, 726
TauI GCSGC 1 cut(s) 146
TfiI GAWTC 1 cut(s) 99
Tru1I TTAA 4 cut(s) 630, 732, 774, 924
Tru9I TTAA 4 cut(s) 630, 732, 774, 924
TscAI CASTG 2 cut(s) 36, 814
TseFI GTSAC 1 cut(s) 233
TseI GCWGC 3 cut(s) 140, 554, 644
Tsp45I GTSAC 1 cut(s) 233
TspDTI ATGAA 3 cut(s) 13, 443, 471
TspGWI ACGGA 2 cut(s) 111, 775
TspRI CASTG 2 cut(s) 36, 814
Tth111I GACNNNGTC 2 cut(s) 189, 239
VpaK11BI GGWCC 2 cut(s) 71, 693
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.