FvH4_3g09670

glucan endo-1,3-beta-glucosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0005800
Physical Location & Seq
Forward (+)
5625227 .. 5628001
2775 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g09670.t1

Sequence Viewer

Length: 522 bp
ATGAGAAAGAACACTTGGGTTTTGCAGTACTGCCTGTTGTTCATGGGATGCTATCTTGCTTTAACAATGGGAGCTGGTGTGGAAGAGAAAGTAGATGGTGCAATGCCAGTGACTACATTGTCGCCACCAGAAGGGAACACAACATTCCTTGATGGCACAACATGGTGTGTGGCTCTTGCTGGTGTCTCCCAAGTTGATCTGCAGAATGCATTAGATTGGGCCTGTGGACTTGGAATGGCAGACTGCAAGGCCATCCAAGATGGCGGGCCCTGTTACGATCCAGACACTCTCTTATCTCATGCTTCCTTTGCTTTCAATAGTTACTATCAACAGAATGGGAATTCTGATATTGCTTGCAATTTTGGGGGTACTGCTGCTGTCACTAAATATAACCCAAGTTATGGAAAATGTGTCTATGCTGCTCCTGGATCTGTTGGCTCTGCAGCAGCTCCATCGTTGATCTCCCTTTGGTGGTGGAAACTTGCTCTCCTTTGTTTACTCCCTTTGTATCTAGGAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
X8

Protein Analysis

174

Amino Acids

18.39

Weight (kDa)

4.47

Isoelectric Point (pI)

28.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
X8 PF07983 54 - 125 7.9e-23 X8 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 118
AccB7I CCANNNNNTGG 1 cut(s) 401
AciI CCGC 1 cut(s) 264
AclWI GGATC 2 cut(s) 272, 436
AcsI RAATTY 1 cut(s) 340
AfaI GTAC 2 cut(s) 29, 370
AfiI CCNNNNNNNGG 3 cut(s) 131, 401, 471
AgsI TTSAA 1 cut(s) 316
AjnI CCWGG 1 cut(s) 424
AjuI GAANNNNNNNTTGG 1 cut(s) 30
AluBI AGCT 3 cut(s) 74, 449, 519
AluI AGCT 3 cut(s) 74, 449, 519
Alw26I GTCTC 1 cut(s) 190
AlwI GGATC 2 cut(s) 272, 436
AoxI GGCC 3 cut(s) 219, 249, 266
ApaI GGGCCC 1 cut(s) 270
ApeKI GCWGC 4 cut(s) 374, 419, 443, 446
ApoI RAATTY 1 cut(s) 340
AspS9I GGNCC 3 cut(s) 219, 266, 267
BaeGI GKGCMC 1 cut(s) 270
BanII GRGCYC 1 cut(s) 270
BbvI GCAGC 4 cut(s) 361, 406, 455, 458
BccI CCATC 5 cut(s) 89, 146, 254, 260, 460
BcgI CGANNNNNNTGC 2 cut(s) 435, 469
BciT130I CCWGG 1 cut(s) 426
BcoDI GTCTC 1 cut(s) 190
BfaI CTAG 1 cut(s) 512
BfmI CTRYAG 2 cut(s) 200, 441
BisI GCNGC 4 cut(s) 375, 420, 444, 447
BlsI GCNGC 4 cut(s) 376, 421, 445, 448
BmcAI AGTACT 1 cut(s) 29
Bme1390I CCNGG 1 cut(s) 426
BmgT120I GGNCC 3 cut(s) 219, 266, 267
BmiI GGNNCC 1 cut(s) 268
BmrFI CCNGG 1 cut(s) 426
BmsI GCATC 1 cut(s) 38
BsaXI ACNNNNNCTCC 4 cut(s) 63, 93, 471, 501
Bsc4I CCNNNNNNNGG 3 cut(s) 131, 401, 471
Bse1I ACTGG 1 cut(s) 107
Bse3DI GCAATG 1 cut(s) 108
BseBI CCWGG 1 cut(s) 426
BseGI GGATG 2 cut(s) 53, 252
BseLI CCNNNNNNNGG 3 cut(s) 131, 401, 471
BseMI GCAATG 1 cut(s) 108
BseNI ACTGG 1 cut(s) 107
BseSI GKGCMC 1 cut(s) 270
BseXI GCAGC 4 cut(s) 361, 406, 455, 458
BshFI GGCC 3 cut(s) 221, 251, 268
BslI CCNNNNNNNGG 3 cut(s) 131, 401, 471
BsmAI GTCTC 1 cut(s) 190
BsmI GAATGC 1 cut(s) 211
BsnI GGCC 3 cut(s) 221, 251, 268
Bsp120I GGGCCC 1 cut(s) 266
Bsp1286I GDGCHC 1 cut(s) 270
Bsp143I GATC 4 cut(s) 196, 277, 428, 459
BspACI CCGC 1 cut(s) 264
BspANI GGCC 3 cut(s) 221, 251, 268
BspLI GGNNCC 1 cut(s) 268
BspMAI CTGCAG 2 cut(s) 204, 445
BspPI GGATC 2 cut(s) 272, 436
BsrDI GCAATG 1 cut(s) 108
BsrI ACTGG 1 cut(s) 107
BssMI GATC 4 cut(s) 196, 277, 428, 459
Bst2UI CCWGG 1 cut(s) 426
Bst6I CTCTTC 1 cut(s) 78
BstC8I GCNNGC 2 cut(s) 266, 355
BstF5I GGATG 2 cut(s) 53, 252
BstKTI GATC 4 cut(s) 199, 280, 431, 462
BstMAI GTCTC 1 cut(s) 190
BstMBI GATC 4 cut(s) 196, 277, 428, 459
BstMWI GCNNNNNNNGC 1 cut(s) 308
BstNI CCWGG 1 cut(s) 426
BstSCI CCNGG 1 cut(s) 424
BstSFI CTRYAG 2 cut(s) 200, 441
BstSLI GKGCMC 1 cut(s) 270
BstV1I GCAGC 4 cut(s) 361, 406, 455, 458
BstX2I RGATCY 1 cut(s) 428
BstYI RGATCY 1 cut(s) 428
BsuRI GGCC 3 cut(s) 221, 251, 268
BtsCI GGATG 2 cut(s) 53, 252
BtsIMutI CAGTG 1 cut(s) 114
Cac8I GCNNGC 2 cut(s) 266, 355
Cfr13I GGNCC 3 cut(s) 219, 266, 267
Csp6I GTAC 2 cut(s) 28, 369
CviAII CATG 3 cut(s) 43, 162, 299
CviJI RGCY 8 cut(s) 74, 173, 221, 251, 268, 438, 449, 519
CviKI_1 RGCY 8 cut(s) 74, 173, 221, 251, 268, 438, 449, 519
CviQI GTAC 2 cut(s) 28, 369
DpnI GATC 4 cut(s) 198, 279, 430, 461
DpnII GATC 4 cut(s) 196, 277, 428, 459
DrdI GACNNNNNNGTC 1 cut(s) 118
DseDI GACNNNNNNGTC 1 cut(s) 118
Eam1104I CTCTTC 1 cut(s) 78
EarI CTCTTC 1 cut(s) 78
Eco24I GRGCYC 1 cut(s) 270
EcoO109I RGGNCCY 1 cut(s) 267
EcoRI GAATTC 1 cut(s) 340
EcoRII CCWGG 1 cut(s) 424
EcoT22I ATGCAT 1 cut(s) 211
EcoT38I GRGCYC 1 cut(s) 270
FaeI CATG 3 cut(s) 46, 165, 302
FaiI YATR 6 cut(s) 44, 163, 300, 390, 402, 417
FatI CATG 3 cut(s) 42, 161, 298
FauI CCCGC 1 cut(s) 257
Fnu4HI GCNGC 4 cut(s) 375, 420, 444, 447
FokI GGATG 2 cut(s) 60, 239
FriOI GRGCYC 1 cut(s) 270
Fsp4HI GCNGC 4 cut(s) 375, 420, 444, 447
FspBI CTAG 1 cut(s) 512
GluI GCNGC 4 cut(s) 375, 420, 444, 447
HaeIII GGCC 3 cut(s) 221, 251, 268
Hin1II CATG 3 cut(s) 46, 165, 302
Hpy166II GTNNAC 2 cut(s) 227, 497
Hpy188I TCNGA 1 cut(s) 346
Hpy188III TCNNGA 1 cut(s) 281
Hpy8I GTNNAC 2 cut(s) 227, 497
HpyAV CCTTC 1 cut(s) 125
HpyCH4V TGCA 7 cut(s) 25, 101, 202, 209, 246, 357, 443
HpyF10VI GCNNNNNNNGC 1 cut(s) 308
Hsp92II CATG 3 cut(s) 46, 165, 302
Kzo9I GATC 4 cut(s) 196, 277, 428, 459
LmnI GCTCC 3 cut(s) 71, 427, 454
Lsp1109I GCAGC 4 cut(s) 361, 406, 455, 458
LweI GCATC 1 cut(s) 38
MaeI CTAG 1 cut(s) 512
MaeIII GTNAC 4 cut(s) 109, 272, 320, 379
MalI GATC 4 cut(s) 198, 279, 430, 461
MboI GATC 4 cut(s) 196, 277, 428, 459
MboII GAAGA 1 cut(s) 95
MflI RGATCY 1 cut(s) 428
MhlI GDGCHC 1 cut(s) 270
MluCI AATT 2 cut(s) 340, 358
Mph1103I ATGCAT 1 cut(s) 211
MseI TTAA 1 cut(s) 62
MspR9I CCNGG 1 cut(s) 426
Mva1269I GAATGC 1 cut(s) 211
MvaI CCWGG 1 cut(s) 426
MwoI GCNNNNNNNGC 1 cut(s) 308
NdeII GATC 4 cut(s) 196, 277, 428, 459
NlaIII CATG 3 cut(s) 46, 165, 302
NlaIV GGNNCC 1 cut(s) 268
NmuCI GTSAC 2 cut(s) 109, 379
NsiI ATGCAT 1 cut(s) 211
PctI GAATGC 1 cut(s) 211
PflMI CCANNNNNTGG 1 cut(s) 401
PfoI TCCNGGA 1 cut(s) 424
PkrI GCNGC 4 cut(s) 376, 421, 445, 448
Psp6I CCWGG 1 cut(s) 424
PspGI CCWGG 1 cut(s) 424
PspN4I GGNNCC 1 cut(s) 268
PspOMI GGGCCC 1 cut(s) 266
PspPI GGNCC 3 cut(s) 219, 266, 267
PstI CTGCAG 2 cut(s) 204, 445
PsuI RGATCY 1 cut(s) 428
RsaI GTAC 2 cut(s) 29, 370
RsaNI GTAC 2 cut(s) 28, 369
SaqAI TTAA 1 cut(s) 62
SatI GCNGC 4 cut(s) 375, 420, 444, 447
Sau3AI GATC 4 cut(s) 196, 277, 428, 459
Sau96I GGNCC 3 cut(s) 219, 266, 267
ScaI AGTACT 1 cut(s) 29
ScrFI CCNGG 1 cut(s) 426
SduI GDGCHC 1 cut(s) 270
SetI ASST 3 cut(s) 76, 451, 521
SfaNI GCATC 1 cut(s) 38
SfcI CTRYAG 2 cut(s) 200, 441
Sse9I AATT 2 cut(s) 340, 358
SsiI CCGC 1 cut(s) 264
SspMI CTAG 1 cut(s) 512
StyD4I CCNGG 1 cut(s) 424
TasI AATT 2 cut(s) 340, 358
TatI WGTACW 1 cut(s) 27
Tru1I TTAA 1 cut(s) 62
Tru9I TTAA 1 cut(s) 62
TscAI CASTG 1 cut(s) 114
TseFI GTSAC 2 cut(s) 109, 379
TseI GCWGC 4 cut(s) 374, 419, 443, 446
Tsp45I GTSAC 2 cut(s) 109, 379
TspDTI ATGAA 1 cut(s) 31
TspRI CASTG 1 cut(s) 114
Van91I CCANNNNNTGG 1 cut(s) 401
XapI RAATTY 1 cut(s) 340
XspI CTAG 1 cut(s) 512
ZrmI AGTACT 1 cut(s) 29
Zsp2I ATGCAT 1 cut(s) 211
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.