Rroxscaffold_1G00061100

glucan endo-1,3-beta-glucosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0005800
Physical Location & Seq
Reverse (-)
83288068 .. 83291122
3055 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00061100.1

Sequence Viewer

Length: 522 bp
ATGAGAAAGAACACTTGGGTTTTGCAGTACTGCCTGTTGTTCATGGGATGCTATCTTGCTTTGACAATGGGAGTTGGTGTGGAAGAGAAAGTAGATGGTGCAATACCAGCGACCACATTGTCACCACCAGAAGGGAACACAACATTCCTTGATGGCACAACATGGTGTGTGGCTCTTGCCGGTGTCCCCCAAGATGATCTGCAGAATGCATTAGATTGGGCCTGTGGGCTTGGAATGGCAGACTGCAAGGCCATCCAAGAAGGCGGGCCCTGTTACGATCCAGACACTCTCTTATCTCATGCTTCCTTCGCTTTCAATAGTTACTATCAACAGAATGGGAATTCTGATATTGCTTGCAATTTTGGGGGCACTGCTGCTGTCACTAAACATAACCCAAGTTATGGAAAATGTGTCTATGCTGCTCCTGGATCTGTAGGCTCTGCAGCACCTCCATTGTTGAGCTTTATTTGGTGGTGGAAACTTGCTCTCCTTTGTTTACTGCTTTTGTATCTAAGAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
X8

Protein Analysis

173

Amino Acids

18.59

Weight (kDa)

4.71

Isoelectric Point (pI)

32.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
X8 PF07983 54 - 125 1.5e-23 X8 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 118
AccB7I CCANNNNNTGG 1 cut(s) 401
AciI CCGC 1 cut(s) 264
AclWI GGATC 2 cut(s) 272, 436
AcsI RAATTY 1 cut(s) 340
AfaI GTAC 1 cut(s) 29
AfiI CCNNNNNNNGG 2 cut(s) 131, 401
AgsI TTSAA 1 cut(s) 316
AjnI CCWGG 1 cut(s) 424
AjuI GAANNNNNNNTTGG 1 cut(s) 30
AluBI AGCT 2 cut(s) 462, 519
AluI AGCT 2 cut(s) 462, 519
AlwI GGATC 2 cut(s) 272, 436
AoxI GGCC 3 cut(s) 219, 249, 266
ApaI GGGCCC 1 cut(s) 270
ApeKI GCWGC 3 cut(s) 374, 419, 443
ApoI RAATTY 1 cut(s) 340
AspS9I GGNCC 3 cut(s) 219, 266, 267
AsuHPI GGTGA 1 cut(s) 114
BaeGI GKGCMC 2 cut(s) 270, 371
BanII GRGCYC 1 cut(s) 270
BbvI GCAGC 3 cut(s) 361, 406, 455
BccI CCATC 3 cut(s) 89, 146, 260
BciT130I CCWGG 1 cut(s) 426
BfmI CTRYAG 3 cut(s) 200, 432, 441
BisI GCNGC 3 cut(s) 375, 420, 444
BlsI GCNGC 3 cut(s) 376, 421, 445
BmcAI AGTACT 1 cut(s) 29
Bme1390I CCNGG 1 cut(s) 426
BmgT120I GGNCC 3 cut(s) 219, 266, 267
BmiI GGNNCC 1 cut(s) 268
BmrFI CCNGG 1 cut(s) 426
BmsI GCATC 1 cut(s) 38
BsaXI ACNNNNNCTCC 4 cut(s) 63, 93, 471, 501
Bsc4I CCNNNNNNNGG 2 cut(s) 131, 401
Bse118I RCCGGY 1 cut(s) 179
BseBI CCWGG 1 cut(s) 426
BseGI GGATG 2 cut(s) 53, 252
BseLI CCNNNNNNNGG 2 cut(s) 131, 401
BseSI GKGCMC 2 cut(s) 270, 371
BseXI GCAGC 3 cut(s) 361, 406, 455
BshFI GGCC 3 cut(s) 221, 251, 268
BsiSI CCGG 1 cut(s) 180
BslFI GGGAC 1 cut(s) 170
BslI CCNNNNNNNGG 2 cut(s) 131, 401
BsmFI GGGAC 1 cut(s) 170
BsmI GAATGC 1 cut(s) 211
BsnI GGCC 3 cut(s) 221, 251, 268
Bsp120I GGGCCC 1 cut(s) 266
Bsp1286I GDGCHC 2 cut(s) 270, 371
Bsp143I GATC 3 cut(s) 196, 277, 428
BspACI CCGC 1 cut(s) 264
BspANI GGCC 3 cut(s) 221, 251, 268
BspLI GGNNCC 1 cut(s) 268
BspMAI CTGCAG 2 cut(s) 204, 445
BspPI GGATC 2 cut(s) 272, 436
BsrFI RCCGGY 1 cut(s) 179
BssAI RCCGGY 1 cut(s) 179
BssMI GATC 3 cut(s) 196, 277, 428
Bst2UI CCWGG 1 cut(s) 426
Bst6I CTCTTC 1 cut(s) 78
BstC8I GCNNGC 2 cut(s) 266, 355
BstDEI CTNAG 1 cut(s) 512
BstF5I GGATG 2 cut(s) 53, 252
BstKTI GATC 3 cut(s) 199, 280, 431
BstMBI GATC 3 cut(s) 196, 277, 428
BstMWI GCNNNNNNNGC 2 cut(s) 107, 308
BstNI CCWGG 1 cut(s) 426
BstSCI CCNGG 1 cut(s) 424
BstSFI CTRYAG 3 cut(s) 200, 432, 441
BstSLI GKGCMC 2 cut(s) 270, 371
BstV1I GCAGC 3 cut(s) 361, 406, 455
BstX2I RGATCY 1 cut(s) 428
BstYI RGATCY 1 cut(s) 428
BsuRI GGCC 3 cut(s) 221, 251, 268
BtsCI GGATG 2 cut(s) 53, 252
BtsI GCAGTG 1 cut(s) 369
BtsIMutI CAGTG 1 cut(s) 369
Cac8I GCNNGC 2 cut(s) 266, 355
Cfr10I RCCGGY 1 cut(s) 179
Cfr13I GGNCC 3 cut(s) 219, 266, 267
Csp6I GTAC 1 cut(s) 28
CviAII CATG 3 cut(s) 43, 162, 299
CviJI RGCY 8 cut(s) 173, 221, 229, 251, 268, 438, 462, 519
CviKI_1 RGCY 8 cut(s) 173, 221, 229, 251, 268, 438, 462, 519
CviQI GTAC 1 cut(s) 28
DdeI CTNAG 1 cut(s) 512
DpnI GATC 3 cut(s) 198, 279, 430
DpnII GATC 3 cut(s) 196, 277, 428
DrdI GACNNNNNNGTC 1 cut(s) 118
DseDI GACNNNNNNGTC 1 cut(s) 118
Eam1104I CTCTTC 1 cut(s) 78
EarI CTCTTC 1 cut(s) 78
Eco24I GRGCYC 1 cut(s) 270
EcoO109I RGGNCCY 1 cut(s) 267
EcoRI GAATTC 1 cut(s) 340
EcoRII CCWGG 1 cut(s) 424
EcoT22I ATGCAT 1 cut(s) 211
EcoT38I GRGCYC 1 cut(s) 270
FaeI CATG 3 cut(s) 46, 165, 302
FaiI YATR 6 cut(s) 44, 163, 300, 390, 402, 417
FaqI GGGAC 1 cut(s) 170
FatI CATG 3 cut(s) 42, 161, 298
FauI CCCGC 1 cut(s) 257
Fnu4HI GCNGC 3 cut(s) 375, 420, 444
FokI GGATG 2 cut(s) 60, 239
FriOI GRGCYC 1 cut(s) 270
Fsp4HI GCNGC 3 cut(s) 375, 420, 444
GluI GCNGC 3 cut(s) 375, 420, 444
HaeIII GGCC 3 cut(s) 221, 251, 268
HapII CCGG 1 cut(s) 180
Hin1II CATG 3 cut(s) 46, 165, 302
HpaII CCGG 1 cut(s) 180
HphI GGTGA 1 cut(s) 114
Hpy166II GTNNAC 1 cut(s) 497
Hpy188I TCNGA 1 cut(s) 346
Hpy188III TCNNGA 1 cut(s) 281
Hpy8I GTNNAC 1 cut(s) 497
HpyAV CCTTC 3 cut(s) 125, 254, 316
HpyCH4V TGCA 7 cut(s) 25, 101, 202, 209, 246, 357, 443
HpyF10VI GCNNNNNNNGC 2 cut(s) 107, 308
HpyF3I CTNAG 1 cut(s) 512
Hsp92II CATG 3 cut(s) 46, 165, 302
Kzo9I GATC 3 cut(s) 196, 277, 428
LmnI GCTCC 1 cut(s) 427
LpnPI CCDG 9 cut(s) 47, 120, 141, 193, 235, 283, 294, 411, 438
Lsp1109I GCAGC 3 cut(s) 361, 406, 455
LweI GCATC 1 cut(s) 38
MaeIII GTNAC 4 cut(s) 120, 272, 320, 379
MalI GATC 3 cut(s) 198, 279, 430
MboI GATC 3 cut(s) 196, 277, 428
MboII GAAGA 1 cut(s) 95
MflI RGATCY 1 cut(s) 428
MhlI GDGCHC 2 cut(s) 270, 371
MluCI AATT 2 cut(s) 340, 358
MnlI CCTC 1 cut(s) 459
Mph1103I ATGCAT 1 cut(s) 211
MspI CCGG 1 cut(s) 180
MspR9I CCNGG 1 cut(s) 426
Mva1269I GAATGC 1 cut(s) 211
MvaI CCWGG 1 cut(s) 426
MwoI GCNNNNNNNGC 2 cut(s) 107, 308
NdeII GATC 3 cut(s) 196, 277, 428
NlaIII CATG 3 cut(s) 46, 165, 302
NlaIV GGNNCC 1 cut(s) 268
NmuCI GTSAC 2 cut(s) 120, 379
NsiI ATGCAT 1 cut(s) 211
PctI GAATGC 1 cut(s) 211
PflMI CCANNNNNTGG 1 cut(s) 401
PfoI TCCNGGA 1 cut(s) 424
PkrI GCNGC 3 cut(s) 376, 421, 445
Psp6I CCWGG 1 cut(s) 424
PspGI CCWGG 1 cut(s) 424
PspN4I GGNNCC 1 cut(s) 268
PspOMI GGGCCC 1 cut(s) 266
PspPI GGNCC 3 cut(s) 219, 266, 267
PstI CTGCAG 2 cut(s) 204, 445
PsuI RGATCY 1 cut(s) 428
RsaI GTAC 1 cut(s) 29
RsaNI GTAC 1 cut(s) 28
SatI GCNGC 3 cut(s) 375, 420, 444
Sau3AI GATC 3 cut(s) 196, 277, 428
Sau96I GGNCC 3 cut(s) 219, 266, 267
ScaI AGTACT 1 cut(s) 29
ScrFI CCNGG 1 cut(s) 426
SduI GDGCHC 2 cut(s) 270, 371
SetI ASST 3 cut(s) 451, 464, 521
SfaNI GCATC 1 cut(s) 38
SfcI CTRYAG 3 cut(s) 200, 432, 441
Sse9I AATT 2 cut(s) 340, 358
SsiI CCGC 1 cut(s) 264
StyD4I CCNGG 1 cut(s) 424
TasI AATT 2 cut(s) 340, 358
TatI WGTACW 1 cut(s) 27
TscAI CASTG 1 cut(s) 376
TseFI GTSAC 2 cut(s) 120, 379
TseI GCWGC 3 cut(s) 374, 419, 443
Tsp45I GTSAC 2 cut(s) 120, 379
TspDTI ATGAA 1 cut(s) 31
TspRI CASTG 1 cut(s) 376
Van91I CCANNNNNTGG 1 cut(s) 401
XapI RAATTY 1 cut(s) 340
ZrmI AGTACT 1 cut(s) 29
Zsp2I ATGCAT 1 cut(s) 211
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.