MD05G1276200.v1.1

glucan endo-1,3-beta-glucosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0005800
Physical Location & Seq
Reverse (-)
41058782 .. 41060871
2090 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1276200.v1.1.491

Sequence Viewer

Length: 540 bp
ATGATGAGAAAGAAGAAATGGGTTTTGGTTTTGCAGTGCCTCTTGCTCATGGGAATGGGATGCCATCTGGTTTCGACAATCGGAGCAGCGGTGCAAGAGAAGGCAGATGGAGCAATTCCAGACACCACATTGTCACCACCAGAAGGGAACACAACATTCCTTGATGGCACGACATGGTGCGTAGCCCTTCCCGGGGTTTCCCAAGCCGATTTGCAGAATGCATTAGACTGGGCCTGCGGATTGGGAATGGTAGACTGCAAGCCAATTCAAAAAGGTGGAGCGTGTTATGAACCAGATACTCTGGTGTCTCATGCCTCTTATGCCTTCAATAATTATTATCAGCAGAACGGGAATTCGGACATTGCTTGCAATTTTGGAGGAACTGCAGGTGTTGCTAAAAAGAACCCAAGTCATGGAAAATGCAACTACGCTGCGCCCGGATCTGTAGCCTCTGCAGCACCTCCACTCTCAAAGCAAAGTCCCATTTCTCTTTGGTGGCGACTTGCTGGCCTTCTGCTGCCATTGTATCTCGGAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
X8

Protein Analysis

180

Amino Acids

18.86

Weight (kDa)

7.53

Isoelectric Point (pI)

36.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
X8 PF07983 58 - 129 2.5e-24 X8 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 377
Acc36I ACCTGC 1 cut(s) 377
AccB7I CCANNNNNTGG 1 cut(s) 413
AccI GTMKAC 1 cut(s) 252
AciI CCGC 2 cut(s) 89, 237
AclWI GGATC 1 cut(s) 448
AcsI RAATTY 1 cut(s) 352
AfiI CCNNNNNNNGG 4 cut(s) 143, 192, 193, 413
AgsI TTSAA 2 cut(s) 269, 328
AjuI GAANNNNNNNTTGG 2 cut(s) 8, 40
AluBI AGCT 1 cut(s) 537
AluI AGCT 1 cut(s) 537
Alw26I GTCTC 1 cut(s) 312
AlwI GGATC 1 cut(s) 448
Ama87I CYCGRG 1 cut(s) 191
AoxI GGCC 2 cut(s) 231, 508
ApeKI GCWGC 4 cut(s) 86, 431, 455, 517
ApoI RAATTY 1 cut(s) 352
AspLEI GCGC 1 cut(s) 436
AspS9I GGNCC 1 cut(s) 231
AsuC2I CCSGG 3 cut(s) 192, 193, 438
AsuHPI GGTGA 1 cut(s) 126
AvaI CYCGRG 1 cut(s) 191
BaeI ACNNNNGTAYC 2 cut(s) 288, 321
BbvI GCAGC 4 cut(s) 98, 418, 467, 504
BccI CCATC 3 cut(s) 72, 101, 158
BcnI CCSGG 3 cut(s) 192, 193, 438
BcoDI GTCTC 1 cut(s) 312
BfmI CTRYAG 3 cut(s) 384, 444, 453
BfuAI ACCTGC 1 cut(s) 377
BisI GCNGC 4 cut(s) 87, 432, 456, 518
BlsI GCNGC 4 cut(s) 88, 433, 457, 519
Bme1390I CCNGG 3 cut(s) 192, 193, 438
BmeT110I CYCGRG 1 cut(s) 191
BmgT120I GGNCC 1 cut(s) 231
BmrFI CCNGG 3 cut(s) 192, 193, 438
BmrI ACTGGG 1 cut(s) 238
BmsI GCATC 1 cut(s) 50
BmuI ACTGGG 1 cut(s) 238
BpuMI CCSGG 3 cut(s) 192, 193, 438
BsaJI CCNNGG 2 cut(s) 191, 192
BsaXI ACNNNNNCTCC 2 cut(s) 75, 105
Bsc4I CCNNNNNNNGG 4 cut(s) 143, 192, 193, 413
Bse1I ACTGG 1 cut(s) 233
Bse3DI GCAATG 1 cut(s) 360
BseDI CCNNGG 2 cut(s) 191, 192
BseGI GGATG 1 cut(s) 65
BseLI CCNNNNNNNGG 4 cut(s) 143, 192, 193, 413
BseMI GCAATG 1 cut(s) 360
BseNI ACTGG 1 cut(s) 233
BseXI GCAGC 4 cut(s) 98, 418, 467, 504
BshFI GGCC 2 cut(s) 233, 510
BsiHKCI CYCGRG 1 cut(s) 191
BsiSI CCGG 2 cut(s) 192, 438
BslFI GGGAC 1 cut(s) 465
BslI CCNNNNNNNGG 4 cut(s) 143, 192, 193, 413
BsmAI GTCTC 1 cut(s) 312
BsmFI GGGAC 1 cut(s) 465
BsmI GAATGC 1 cut(s) 223
BsnI GGCC 2 cut(s) 233, 510
BsoBI CYCGRG 1 cut(s) 191
Bsp143I GATC 1 cut(s) 440
BspACI CCGC 2 cut(s) 89, 237
BspANI GGCC 2 cut(s) 233, 510
BspMAI CTGCAG 2 cut(s) 388, 457
BspMI ACCTGC 1 cut(s) 377
BspPI GGATC 1 cut(s) 448
BsrDI GCAATG 1 cut(s) 360
BsrI ACTGG 1 cut(s) 233
BssECI CCNNGG 2 cut(s) 191, 192
BssMI GATC 1 cut(s) 440
BstAPI GCANNNNNTGC 1 cut(s) 392
BstC8I GCNNGC 4 cut(s) 235, 260, 367, 508
BstF5I GGATG 1 cut(s) 65
BstHHI GCGC 1 cut(s) 436
BstKTI GATC 1 cut(s) 443
BstMAI GTCTC 1 cut(s) 312
BstMBI GATC 1 cut(s) 440
BstMWI GCNNNNNNNGC 4 cut(s) 110, 320, 392, 455
BstSCI CCNGG 3 cut(s) 190, 191, 436
BstSFI CTRYAG 3 cut(s) 384, 444, 453
BstV1I GCAGC 4 cut(s) 98, 418, 467, 504
BstX2I RGATCY 1 cut(s) 440
BstYI RGATCY 1 cut(s) 440
BsuRI GGCC 2 cut(s) 233, 510
BtsCI GGATG 1 cut(s) 65
BtsI GCAGTG 1 cut(s) 41
BtsIMutI CAGTG 1 cut(s) 41
BveI ACCTGC 1 cut(s) 377
Cac8I GCNNGC 4 cut(s) 235, 260, 367, 508
CfoI GCGC 1 cut(s) 436
Cfr13I GGNCC 1 cut(s) 231
Cfr9I CCCGGG 1 cut(s) 191
CviAII CATG 4 cut(s) 49, 174, 311, 413
CviJI RGCY 7 cut(s) 185, 206, 233, 262, 449, 510, 537
CviKI_1 RGCY 7 cut(s) 185, 206, 233, 262, 449, 510, 537
DpnI GATC 1 cut(s) 442
DpnII GATC 1 cut(s) 440
Eco88I CYCGRG 1 cut(s) 191
EcoRI GAATTC 1 cut(s) 352
EcoT22I ATGCAT 1 cut(s) 223
FaeI CATG 4 cut(s) 52, 177, 314, 416
FaiI YATR 6 cut(s) 50, 175, 288, 312, 321, 414
FaqI GGGAC 1 cut(s) 465
FatI CATG 4 cut(s) 48, 173, 310, 412
FblI GTMKAC 1 cut(s) 252
Fnu4HI GCNGC 4 cut(s) 87, 432, 456, 518
FokI GGATG 1 cut(s) 72
Fsp4HI GCNGC 4 cut(s) 87, 432, 456, 518
GlaI GCGC 1 cut(s) 435
GluI GCNGC 4 cut(s) 87, 432, 456, 518
HaeIII GGCC 2 cut(s) 233, 510
HapII CCGG 2 cut(s) 192, 438
HhaI GCGC 1 cut(s) 436
Hin1II CATG 4 cut(s) 52, 177, 314, 416
Hin6I GCGC 1 cut(s) 434
HinP1I GCGC 1 cut(s) 434
HpaII CCGG 2 cut(s) 192, 438
HphI GGTGA 1 cut(s) 126
Hpy166II GTNNAC 1 cut(s) 253
Hpy188I TCNGA 3 cut(s) 83, 358, 533
Hpy188III TCNNGA 1 cut(s) 119
Hpy8I GTNNAC 1 cut(s) 253
HpyAV CCTTC 5 cut(s) 94, 137, 197, 334, 521
HpyCH4V TGCA 9 cut(s) 34, 94, 214, 221, 258, 369, 386, 423, 455
HpyF10VI GCNNNNNNNGC 4 cut(s) 110, 320, 392, 455
Hsp92II CATG 4 cut(s) 52, 177, 314, 416
HspAI GCGC 1 cut(s) 434
Kzo9I GATC 1 cut(s) 440
LmnI GCTCC 3 cut(s) 83, 110, 278
Lsp1109I GCAGC 4 cut(s) 98, 418, 467, 504
LweI GCATC 1 cut(s) 50
MaeIII GTNAC 1 cut(s) 132
MalI GATC 1 cut(s) 442
MboI GATC 1 cut(s) 440
MboII GAAGA 1 cut(s) 25
MflI RGATCY 1 cut(s) 440
MluCI AATT 5 cut(s) 114, 264, 331, 352, 370
MnlI CCTC 5 cut(s) 50, 325, 371, 460, 471
Mph1103I ATGCAT 1 cut(s) 223
MslI CAYNNNNRTG 1 cut(s) 53
MspA1I CMGCKG 1 cut(s) 89
MspI CCGG 2 cut(s) 192, 438
MspR9I CCNGG 3 cut(s) 192, 193, 438
Mva1269I GAATGC 1 cut(s) 223
MwoI GCNNNNNNNGC 4 cut(s) 110, 320, 392, 455
NciI CCSGG 3 cut(s) 192, 193, 438
NdeII GATC 1 cut(s) 440
NlaIII CATG 4 cut(s) 52, 177, 314, 416
NmuCI GTSAC 1 cut(s) 132
NsiI ATGCAT 1 cut(s) 223
PaqCI CACCTGC 1 cut(s) 377
PctI GAATGC 1 cut(s) 223
PflMI CCANNNNNTGG 1 cut(s) 413
PkrI GCNGC 4 cut(s) 88, 433, 457, 519
PspPI GGNCC 1 cut(s) 231
PstI CTGCAG 2 cut(s) 388, 457
PsuI RGATCY 1 cut(s) 440
RseI CAYNNNNRTG 1 cut(s) 53
SatI GCNGC 4 cut(s) 87, 432, 456, 518
Sau3AI GATC 1 cut(s) 440
Sau96I GGNCC 1 cut(s) 231
ScrFI CCNGG 3 cut(s) 192, 193, 438
SetI ASST 4 cut(s) 277, 391, 463, 539
SfaNI GCATC 1 cut(s) 50
SfcI CTRYAG 3 cut(s) 384, 444, 453
SmaI CCCGGG 1 cut(s) 193
SmiMI CAYNNNNRTG 1 cut(s) 53
Sse9I AATT 5 cut(s) 114, 264, 331, 352, 370
SsiI CCGC 2 cut(s) 89, 237
StyD4I CCNGG 3 cut(s) 190, 191, 436
TaqI TCGA 1 cut(s) 74
TasI AATT 5 cut(s) 114, 264, 331, 352, 370
TscAI CASTG 1 cut(s) 41
TseFI GTSAC 1 cut(s) 132
TseI GCWGC 4 cut(s) 86, 431, 455, 517
Tsp45I GTSAC 1 cut(s) 132
TspDTI ATGAA 1 cut(s) 303
TspMI CCCGGG 1 cut(s) 191
TspRI CASTG 1 cut(s) 41
Van91I CCANNNNNTGG 1 cut(s) 413
XapI RAATTY 1 cut(s) 352
XmaI CCCGGG 1 cut(s) 191
XmiI GTMKAC 1 cut(s) 252
Zsp2I ATGCAT 1 cut(s) 223
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.