pycom05g25720

glucan endo-1,3-beta-glucosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0005800
Physical Location & Seq
Reverse (-)
27063054 .. 27065790
2737 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g25720.1

Sequence Viewer

Length: 654 bp
ATGTCCGATATAGTAGTAGAAATAGAGAGAGATAGAGAGAGGGAGAGTGGTGGGTTTTCTTATATTTTTATTTTTATTTTAGTATTATATTTGTTTTCTAGGAGAAGGGTGTCAAGCATGAGCAAGAAGATATGGGTTTTGGTTTTGCAGTGCCTCTTGCTCGTGGGAATGGGATGCCAACTGGTTTCGACAATGGGAGCAGCGGTGCAAGAGAAGGCAGATGGAGCAATTCCAGACACCACATTGTCACCACCAGAAGGGAACACAACATTCCTTGATGGCACGACATGGTGCGTAGCCCTTCCCGGGGTTTCCCAAGCCGATTTGCAGAACGCATTAGACTGGGCCTGCGGATTGGGAATGGCAGACTGCAAGCCAATTCAAAAGGGTGGACCGTGTTACGAACCAGATACTCTGGTGTCTCATGCCTCTTATGCCTTCAATAATTATTATCAGCAGAACGGGAATTCGGACATTGCTTGCAATTTTGGAGGAACTGCAGGTGTTACTAAAAAGAACCCAAGTCATGGAAAATGCAACTACGCTGCGCCCGGATCTGTAGGCTCTGCAGCACCTCCATTCTCAAAGCAAAGTCCCATCTCTCTTTGGTGGCAACTTGCTGGCCTTCTGCTGCCATTGTATCTCGGAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
X8

Protein Analysis

218

Amino Acids

23.31

Weight (kDa)

5.64

Isoelectric Point (pI)

43.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
X8 PF07983 96 - 167 4.3e-24 X8 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 491
Acc36I ACCTGC 1 cut(s) 491
AccB7I CCANNNNNTGG 1 cut(s) 527
AciI CCGC 2 cut(s) 203, 351
AclWI GGATC 1 cut(s) 562
AcsI RAATTY 1 cut(s) 466
AfiI CCNNNNNNNGG 4 cut(s) 257, 306, 307, 527
AgsI TTSAA 2 cut(s) 383, 442
AluBI AGCT 1 cut(s) 651
AluI AGCT 1 cut(s) 651
Alw26I GTCTC 1 cut(s) 426
AlwI GGATC 1 cut(s) 562
Ama87I CYCGRG 1 cut(s) 305
AoxI GGCC 2 cut(s) 345, 622
ApeKI GCWGC 4 cut(s) 200, 545, 569, 631
ApoI RAATTY 1 cut(s) 466
AspLEI GCGC 1 cut(s) 550
AspS9I GGNCC 2 cut(s) 345, 392
AsuC2I CCSGG 3 cut(s) 306, 307, 552
AsuHPI GGTGA 1 cut(s) 240
AvaI CYCGRG 1 cut(s) 305
AvaII GGWCC 1 cut(s) 392
BaeI ACNNNNGTAYC 2 cut(s) 402, 435
BauI CACGAG 1 cut(s) 161
BbvI GCAGC 4 cut(s) 212, 532, 581, 618
BccI CCATC 3 cut(s) 215, 272, 605
BcnI CCSGG 3 cut(s) 306, 307, 552
BcoDI GTCTC 1 cut(s) 426
BfaI CTAG 1 cut(s) 99
BfmI CTRYAG 3 cut(s) 498, 558, 567
BfuAI ACCTGC 1 cut(s) 491
BisI GCNGC 4 cut(s) 201, 546, 570, 632
BlsI GCNGC 4 cut(s) 202, 547, 571, 633
Bme1390I CCNGG 3 cut(s) 306, 307, 552
Bme18I GGWCC 1 cut(s) 392
BmeT110I CYCGRG 1 cut(s) 305
BmgT120I GGNCC 2 cut(s) 345, 392
BmrFI CCNGG 3 cut(s) 306, 307, 552
BmrI ACTGGG 1 cut(s) 352
BmsI GCATC 1 cut(s) 164
BmuI ACTGGG 1 cut(s) 352
BpuMI CCSGG 3 cut(s) 306, 307, 552
BsaJI CCNNGG 2 cut(s) 305, 306
BsaXI ACNNNNNCTCC 2 cut(s) 189, 219
Bsc4I CCNNNNNNNGG 4 cut(s) 257, 306, 307, 527
Bse1I ACTGG 2 cut(s) 186, 347
Bse3DI GCAATG 1 cut(s) 474
BseDI CCNNGG 2 cut(s) 305, 306
BseGI GGATG 1 cut(s) 179
BseLI CCNNNNNNNGG 4 cut(s) 257, 306, 307, 527
BseMI GCAATG 1 cut(s) 474
BseNI ACTGG 2 cut(s) 186, 347
BseXI GCAGC 4 cut(s) 212, 532, 581, 618
BshFI GGCC 2 cut(s) 347, 624
BsiHKCI CYCGRG 1 cut(s) 305
BsiSI CCGG 2 cut(s) 306, 552
BslFI GGGAC 1 cut(s) 579
BslI CCNNNNNNNGG 4 cut(s) 257, 306, 307, 527
BsmAI GTCTC 1 cut(s) 426
BsmFI GGGAC 1 cut(s) 579
BsnI GGCC 2 cut(s) 347, 624
BsoBI CYCGRG 1 cut(s) 305
Bsp143I GATC 1 cut(s) 554
BspACI CCGC 2 cut(s) 203, 351
BspANI GGCC 2 cut(s) 347, 624
BspMAI CTGCAG 2 cut(s) 502, 571
BspMI ACCTGC 1 cut(s) 491
BspPI GGATC 1 cut(s) 562
BsrDI GCAATG 1 cut(s) 474
BsrI ACTGG 2 cut(s) 186, 347
BssECI CCNNGG 2 cut(s) 305, 306
BssMI GATC 1 cut(s) 554
BssSI CACGAG 1 cut(s) 161
Bst2BI CACGAG 1 cut(s) 161
Bst4CI ACNGT 1 cut(s) 396
BstC8I GCNNGC 4 cut(s) 349, 374, 481, 622
BstF5I GGATG 1 cut(s) 179
BstHHI GCGC 1 cut(s) 550
BstKTI GATC 1 cut(s) 557
BstMAI GTCTC 1 cut(s) 426
BstMBI GATC 1 cut(s) 554
BstMWI GCNNNNNNNGC 2 cut(s) 224, 434
BstSCI CCNGG 3 cut(s) 304, 305, 550
BstSFI CTRYAG 3 cut(s) 498, 558, 567
BstV1I GCAGC 4 cut(s) 212, 532, 581, 618
BstX2I RGATCY 1 cut(s) 554
BstYI RGATCY 1 cut(s) 554
BsuRI GGCC 2 cut(s) 347, 624
BtsCI GGATG 1 cut(s) 179
BtsI GCAGTG 1 cut(s) 155
BtsIMutI CAGTG 1 cut(s) 155
BveI ACCTGC 1 cut(s) 491
Cac8I GCNNGC 4 cut(s) 349, 374, 481, 622
CfoI GCGC 1 cut(s) 550
Cfr13I GGNCC 2 cut(s) 345, 392
Cfr9I CCCGGG 1 cut(s) 305
CviAII CATG 4 cut(s) 118, 288, 425, 527
CviJI RGCY 7 cut(s) 299, 320, 347, 376, 564, 624, 651
CviKI_1 RGCY 7 cut(s) 299, 320, 347, 376, 564, 624, 651
DpnI GATC 1 cut(s) 556
DpnII GATC 1 cut(s) 554
Eco47I GGWCC 1 cut(s) 392
Eco88I CYCGRG 1 cut(s) 305
EcoRI GAATTC 1 cut(s) 466
FaeI CATG 4 cut(s) 121, 291, 428, 530
FaiI YATR 9 cut(s) 11, 63, 88, 119, 133, 289, 426, 435, 528
FaqI GGGAC 1 cut(s) 579
FatI CATG 4 cut(s) 117, 287, 424, 526
Fnu4HI GCNGC 4 cut(s) 201, 546, 570, 632
FokI GGATG 1 cut(s) 186
Fsp4HI GCNGC 4 cut(s) 201, 546, 570, 632
FspBI CTAG 1 cut(s) 99
GlaI GCGC 1 cut(s) 549
GluI GCNGC 4 cut(s) 201, 546, 570, 632
HaeIII GGCC 2 cut(s) 347, 624
HapII CCGG 2 cut(s) 306, 552
HhaI GCGC 1 cut(s) 550
Hin1II CATG 4 cut(s) 121, 291, 428, 530
Hin6I GCGC 1 cut(s) 548
HinP1I GCGC 1 cut(s) 548
HpaII CCGG 2 cut(s) 306, 552
HphI GGTGA 1 cut(s) 240
Hpy166II GTNNAC 1 cut(s) 392
Hpy188I TCNGA 3 cut(s) 7, 472, 647
Hpy188III TCNNGA 1 cut(s) 233
Hpy8I GTNNAC 1 cut(s) 392
HpyAV CCTTC 6 cut(s) 99, 208, 251, 311, 448, 635
HpyCH4III ACNGT 1 cut(s) 396
HpyCH4V TGCA 8 cut(s) 148, 208, 328, 372, 483, 500, 537, 569
HpyF10VI GCNNNNNNNGC 2 cut(s) 224, 434
Hsp92II CATG 4 cut(s) 121, 291, 428, 530
HspAI GCGC 1 cut(s) 548
Kzo9I GATC 1 cut(s) 554
LmnI GCTCC 2 cut(s) 197, 224
Lsp1109I GCAGC 4 cut(s) 212, 532, 581, 618
LweI GCATC 1 cut(s) 164
MaeI CTAG 1 cut(s) 99
MaeIII GTNAC 3 cut(s) 246, 398, 505
MalI GATC 1 cut(s) 556
MboI GATC 1 cut(s) 554
MboII GAAGA 1 cut(s) 139
MflI RGATCY 1 cut(s) 554
MluCI AATT 5 cut(s) 228, 378, 445, 466, 484
MnlI CCTC 5 cut(s) 33, 164, 439, 485, 585
MspA1I CMGCKG 1 cut(s) 203
MspI CCGG 2 cut(s) 306, 552
MspR9I CCNGG 3 cut(s) 306, 307, 552
MwoI GCNNNNNNNGC 2 cut(s) 224, 434
NciI CCSGG 3 cut(s) 306, 307, 552
NdeII GATC 1 cut(s) 554
NlaIII CATG 4 cut(s) 121, 291, 428, 530
NmuCI GTSAC 1 cut(s) 246
PaqCI CACCTGC 1 cut(s) 491
PflMI CCANNNNNTGG 1 cut(s) 527
PkrI GCNGC 4 cut(s) 202, 547, 571, 633
PspPI GGNCC 2 cut(s) 345, 392
PstI CTGCAG 2 cut(s) 502, 571
PsuI RGATCY 1 cut(s) 554
SatI GCNGC 4 cut(s) 201, 546, 570, 632
Sau3AI GATC 1 cut(s) 554
Sau96I GGNCC 2 cut(s) 345, 392
ScrFI CCNGG 3 cut(s) 306, 307, 552
SetI ASST 3 cut(s) 505, 577, 653
SfaNI GCATC 1 cut(s) 164
SfcI CTRYAG 3 cut(s) 498, 558, 567
SinI GGWCC 1 cut(s) 392
SmaI CCCGGG 1 cut(s) 307
Sse9I AATT 5 cut(s) 228, 378, 445, 466, 484
SsiI CCGC 2 cut(s) 203, 351
SspMI CTAG 1 cut(s) 99
StyD4I CCNGG 3 cut(s) 304, 305, 550
TaaI ACNGT 1 cut(s) 396
TaqI TCGA 1 cut(s) 188
TasI AATT 5 cut(s) 228, 378, 445, 466, 484
TscAI CASTG 1 cut(s) 155
TseFI GTSAC 1 cut(s) 246
TseI GCWGC 4 cut(s) 200, 545, 569, 631
Tsp45I GTSAC 1 cut(s) 246
TspMI CCCGGG 1 cut(s) 305
TspRI CASTG 1 cut(s) 155
Van91I CCANNNNNTGG 1 cut(s) 527
VpaK11BI GGWCC 1 cut(s) 392
XapI RAATTY 1 cut(s) 466
XmaI CCCGGG 1 cut(s) 305
XspI CTAG 1 cut(s) 99
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.