Rh5AG120300

glucan endo-1,3-beta-glucosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0005800
Physical Location & Seq
Forward (+)
11225563 .. 11228636
3074 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG120300.1

Sequence Viewer

Length: 621 bp
ATGAGAAAGAACACTTGGGTTTTGCAGTGCTGCCTGTTGTTCATAGGATGCTATCTTGCTTTGACAATGGGAGTTGGTGTGGAAGAGAAAGTAGATGGTGCAATACCAGTGACCACATTGTCACCACCAGAAGGGAACACAACATTCCTTGATGGCACAACATGGTGTGTGGCTCTTGCTGGTGTCCCCCAAGATGATCTGCAGAATGCATTAGATTGGGCCTGTGGGCTCGGAATGGCAGACTGCAAGGCCATCCAAGAAGGCGGGCCCTGTTACAATCCAGACACTCTCTTATCTCATGCTTCCTTCGCTTTCAATAGCTACTATCAACAGAATGGGAATTCTGATATTGCTTGCAATTTTGGGGGCACTGCTGCTGTCACTAAACATAACCCAAGTTATGGAAAATGTGTCTATGCTGCTCCTGGGTATACTTGTTCTCACCTACATCTTTTAGTTTGCCTAATGCATTATTTACTAAATTGTACGCGTTGTTTGATTTTTAATATATATTTCTCGTCTTCCAGATCTGTAGGCTCTGCAGCACCTCCATTGTTGAGCTTTATTTGGTGGTGGAAACTTGCTCTCCTTTGTTTACTGCTTTTGTATCTAAGAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
X8

Protein Analysis

206

Amino Acids

22.48

Weight (kDa)

6.15

Isoelectric Point (pI)

41.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
X8 PF07983 54 - 125 1.5e-23 X8 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 118
AccB7I CCANNNNNTGG 1 cut(s) 401
AccI GTMKAC 1 cut(s) 431
AccII CGCG 1 cut(s) 490
AciI CCGC 1 cut(s) 264
AcsI RAATTY 1 cut(s) 340
AfaI GTAC 1 cut(s) 487
AfiI CCNNNNNNNGG 2 cut(s) 131, 401
AflIII ACRYGT 1 cut(s) 488
AgsI TTSAA 1 cut(s) 316
AjnI CCWGG 1 cut(s) 424
AjuI GAANNNNNNNTTGG 1 cut(s) 30
AluBI AGCT 3 cut(s) 321, 561, 618
AluI AGCT 3 cut(s) 321, 561, 618
AoxI GGCC 3 cut(s) 219, 249, 266
ApaI GGGCCC 1 cut(s) 270
ApeKI GCWGC 4 cut(s) 30, 374, 419, 542
ApoI RAATTY 1 cut(s) 340
AspS9I GGNCC 3 cut(s) 219, 266, 267
AsuHPI GGTGA 2 cut(s) 114, 434
BaeGI GKGCMC 2 cut(s) 270, 371
BanII GRGCYC 2 cut(s) 231, 270
BbsI GAAGAC 1 cut(s) 513
BbvI GCAGC 4 cut(s) 17, 361, 406, 554
BccI CCATC 3 cut(s) 89, 146, 260
BciT130I CCWGG 1 cut(s) 426
BfmI CTRYAG 3 cut(s) 200, 531, 540
BglII AGATCT 1 cut(s) 527
BisI GCNGC 4 cut(s) 31, 375, 420, 543
BlsI GCNGC 4 cut(s) 32, 376, 421, 544
Bme1390I CCNGG 1 cut(s) 426
BmgT120I GGNCC 3 cut(s) 219, 266, 267
BmiI GGNNCC 1 cut(s) 268
BmrFI CCNGG 1 cut(s) 426
BmsI GCATC 1 cut(s) 38
BpiI GAAGAC 1 cut(s) 513
BsaJI CCNNGG 1 cut(s) 425
BsaXI ACNNNNNCTCC 4 cut(s) 63, 93, 570, 600
Bsc4I CCNNNNNNNGG 2 cut(s) 131, 401
Bse1I ACTGG 1 cut(s) 107
BseBI CCWGG 1 cut(s) 426
BseDI CCNNGG 1 cut(s) 425
BseGI GGATG 2 cut(s) 53, 252
BseLI CCNNNNNNNGG 2 cut(s) 131, 401
BseNI ACTGG 1 cut(s) 107
BseSI GKGCMC 2 cut(s) 270, 371
BseXI GCAGC 4 cut(s) 17, 361, 406, 554
Bsh1236I CGCG 1 cut(s) 490
BshFI GGCC 3 cut(s) 221, 251, 268
BslFI GGGAC 1 cut(s) 170
BslI CCNNNNNNNGG 2 cut(s) 131, 401
BsmFI GGGAC 1 cut(s) 170
BsmI GAATGC 1 cut(s) 211
BsnI GGCC 3 cut(s) 221, 251, 268
Bsp120I GGGCCC 1 cut(s) 266
Bsp1286I GDGCHC 3 cut(s) 231, 270, 371
Bsp143I GATC 2 cut(s) 196, 527
BspACI CCGC 1 cut(s) 264
BspANI GGCC 3 cut(s) 221, 251, 268
BspFNI CGCG 1 cut(s) 490
BspLI GGNNCC 1 cut(s) 268
BspMAI CTGCAG 2 cut(s) 204, 544
BsrI ACTGG 1 cut(s) 107
BssECI CCNNGG 1 cut(s) 425
BssMI GATC 2 cut(s) 196, 527
BssNAI GTATAC 1 cut(s) 432
Bst1107I GTATAC 1 cut(s) 432
Bst2UI CCWGG 1 cut(s) 426
Bst6I CTCTTC 1 cut(s) 78
BstC8I GCNNGC 2 cut(s) 266, 355
BstDEI CTNAG 1 cut(s) 611
BstF5I GGATG 2 cut(s) 53, 252
BstFNI CGCG 1 cut(s) 490
BstKTI GATC 2 cut(s) 199, 530
BstMBI GATC 2 cut(s) 196, 527
BstMWI GCNNNNNNNGC 1 cut(s) 308
BstNI CCWGG 1 cut(s) 426
BstSCI CCNGG 1 cut(s) 424
BstSFI CTRYAG 3 cut(s) 200, 531, 540
BstSLI GKGCMC 2 cut(s) 270, 371
BstUI CGCG 1 cut(s) 490
BstV1I GCAGC 4 cut(s) 17, 361, 406, 554
BstV2I GAAGAC 1 cut(s) 513
BstX2I RGATCY 1 cut(s) 527
BstYI RGATCY 1 cut(s) 527
BstZ17I GTATAC 1 cut(s) 432
BsuRI GGCC 3 cut(s) 221, 251, 268
BtsCI GGATG 2 cut(s) 53, 252
BtsI GCAGTG 2 cut(s) 32, 369
BtsIMutI CAGTG 3 cut(s) 32, 114, 369
Cac8I GCNNGC 2 cut(s) 266, 355
Cfr13I GGNCC 3 cut(s) 219, 266, 267
Csp6I GTAC 1 cut(s) 486
CviAII CATG 2 cut(s) 162, 299
CviJI RGCY 9 cut(s) 173, 221, 229, 251, 268, 321, 537, 561, 618
CviKI_1 RGCY 9 cut(s) 173, 221, 229, 251, 268, 321, 537, 561, 618
CviQI GTAC 1 cut(s) 486
DdeI CTNAG 1 cut(s) 611
DpnI GATC 2 cut(s) 198, 529
DpnII GATC 2 cut(s) 196, 527
DrdI GACNNNNNNGTC 1 cut(s) 118
DseDI GACNNNNNNGTC 1 cut(s) 118
Eam1104I CTCTTC 1 cut(s) 78
EarI CTCTTC 1 cut(s) 78
Eco24I GRGCYC 2 cut(s) 231, 270
EcoO109I RGGNCCY 1 cut(s) 267
EcoRI GAATTC 1 cut(s) 340
EcoRII CCWGG 1 cut(s) 424
EcoT22I ATGCAT 2 cut(s) 211, 471
EcoT38I GRGCYC 2 cut(s) 231, 270
FaeI CATG 2 cut(s) 165, 302
FaiI YATR 9 cut(s) 44, 163, 300, 390, 402, 417, 432, 509, 511
FaqI GGGAC 1 cut(s) 170
FatI CATG 2 cut(s) 161, 298
FauI CCCGC 1 cut(s) 257
FblI GTMKAC 1 cut(s) 431
Fnu4HI GCNGC 4 cut(s) 31, 375, 420, 543
FokI GGATG 2 cut(s) 60, 239
FriOI GRGCYC 2 cut(s) 231, 270
Fsp4HI GCNGC 4 cut(s) 31, 375, 420, 543
GluI GCNGC 4 cut(s) 31, 375, 420, 543
HaeIII GGCC 3 cut(s) 221, 251, 268
Hin1II CATG 2 cut(s) 165, 302
HphI GGTGA 2 cut(s) 114, 434
Hpy166II GTNNAC 2 cut(s) 432, 596
Hpy188I TCNGA 2 cut(s) 233, 346
Hpy188III TCNNGA 2 cut(s) 281, 525
Hpy8I GTNNAC 2 cut(s) 432, 596
HpyAV CCTTC 3 cut(s) 125, 254, 316
HpyCH4V TGCA 8 cut(s) 25, 101, 202, 209, 246, 357, 469, 542
HpyF10VI GCNNNNNNNGC 1 cut(s) 308
HpyF3I CTNAG 1 cut(s) 611
Hsp92II CATG 2 cut(s) 165, 302
Kzo9I GATC 2 cut(s) 196, 527
LmnI GCTCC 1 cut(s) 427
Lsp1109I GCAGC 4 cut(s) 17, 361, 406, 554
LweI GCATC 1 cut(s) 38
MaeIII GTNAC 4 cut(s) 109, 120, 272, 379
MalI GATC 2 cut(s) 198, 529
MboI GATC 2 cut(s) 196, 527
MboII GAAGA 2 cut(s) 95, 513
MflI RGATCY 1 cut(s) 527
MhlI GDGCHC 3 cut(s) 231, 270, 371
MluCI AATT 3 cut(s) 340, 358, 481
MluI ACGCGT 1 cut(s) 488
MnlI CCTC 1 cut(s) 558
Mph1103I ATGCAT 2 cut(s) 211, 471
MseI TTAA 1 cut(s) 504
MspR9I CCNGG 1 cut(s) 426
Mva1269I GAATGC 1 cut(s) 211
MvaI CCWGG 1 cut(s) 426
MvnI CGCG 1 cut(s) 490
MwoI GCNNNNNNNGC 1 cut(s) 308
NdeII GATC 2 cut(s) 196, 527
NlaIII CATG 2 cut(s) 165, 302
NlaIV GGNNCC 1 cut(s) 268
NmuCI GTSAC 3 cut(s) 109, 120, 379
NsiI ATGCAT 2 cut(s) 211, 471
PctI GAATGC 1 cut(s) 211
PflMI CCANNNNNTGG 1 cut(s) 401
PkrI GCNGC 4 cut(s) 32, 376, 421, 544
Psp6I CCWGG 1 cut(s) 424
PspGI CCWGG 1 cut(s) 424
PspN4I GGNNCC 1 cut(s) 268
PspOMI GGGCCC 1 cut(s) 266
PspPI GGNCC 3 cut(s) 219, 266, 267
PstI CTGCAG 2 cut(s) 204, 544
PsuI RGATCY 1 cut(s) 527
RsaI GTAC 1 cut(s) 487
RsaNI GTAC 1 cut(s) 486
SaqAI TTAA 1 cut(s) 504
SatI GCNGC 4 cut(s) 31, 375, 420, 543
Sau3AI GATC 2 cut(s) 196, 527
Sau96I GGNCC 3 cut(s) 219, 266, 267
ScrFI CCNGG 1 cut(s) 426
SduI GDGCHC 3 cut(s) 231, 270, 371
SetI ASST 5 cut(s) 323, 447, 550, 563, 620
SfaNI GCATC 1 cut(s) 38
SfcI CTRYAG 3 cut(s) 200, 531, 540
Sse9I AATT 3 cut(s) 340, 358, 481
SsiI CCGC 1 cut(s) 264
StyD4I CCNGG 1 cut(s) 424
TasI AATT 3 cut(s) 340, 358, 481
Tru1I TTAA 1 cut(s) 504
Tru9I TTAA 1 cut(s) 504
TscAI CASTG 3 cut(s) 32, 114, 376
TseFI GTSAC 3 cut(s) 109, 120, 379
TseI GCWGC 4 cut(s) 30, 374, 419, 542
Tsp45I GTSAC 3 cut(s) 109, 120, 379
TspDTI ATGAA 1 cut(s) 31
TspRI CASTG 3 cut(s) 32, 114, 376
Van91I CCANNNNNTGG 1 cut(s) 401
XapI RAATTY 1 cut(s) 340
XmiI GTMKAC 1 cut(s) 431
Zsp2I ATGCAT 2 cut(s) 211, 471
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.