MD00G1109800.v1.1

glucan endo-1,3-beta-glucosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0005800
Physical Location & Seq
Reverse (-)
22955261 .. 22957742
2482 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1109800.v1.1.491

Sequence Viewer

Length: 537 bp
ATGTTATCATCAAGCATGAGAAAGAAGCTATGGGTTTTGGTTTTGCAGTGCCTTTTGCTCATGGGATGCCATCTGGTTTCATCAATGGAAGTGGCGCTGCAAGATGGAGCAATACCAGACACCACATTGTCACCGCCAGAAGGGAACACAACATTCCTTGATGGCACAACATGGTGCGTAGCCCTTCCCGGGGTTTCCCAAGCCGATTTGCAGAATGCATTAGACTGGGCCTGTGGATTGGGAATGGCAGACTGCAAGCCAATTCAACAAGGTGGAGCGTGTTACGAACCAGATACTCTGGTATCTCATGCCTCTTATGCTTTCAATAATTTTTATCAGCAGAACGGGAATTCAGATATTGCTTGCAATTTTGGGGGAACTGCCGCTGTCACTAAAACTAACCCAAGTCATGGAAAATGCGTCTATGCTGCGCCCGGATCTGTAGGCTCTGCAGCACCTCCATTTTCCAAGCAAAATCCCAGCTCTCTTTGGTGGCAACTTGCTTGCCTTTTACTCCCCTTGTATCTAGGAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
X8

Protein Analysis

179

Amino Acids

18.82

Weight (kDa)

5.06

Isoelectric Point (pI)

45.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
X8 PF07983 57 - 128 1.5e-23 X8 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 410
AciI CCGC 2 cut(s) 134, 384
AclWI GGATC 1 cut(s) 445
AcsI RAATTY 1 cut(s) 349
AfiI CCNNNNNNNGG 4 cut(s) 140, 189, 190, 410
AgsI TTSAA 2 cut(s) 266, 325
AluBI AGCT 2 cut(s) 28, 483
AluI AGCT 2 cut(s) 28, 483
AlwI GGATC 1 cut(s) 445
Ama87I CYCGRG 1 cut(s) 188
AoxI GGCC 1 cut(s) 228
ApeKI GCWGC 3 cut(s) 97, 428, 452
ApoI RAATTY 1 cut(s) 349
AspLEI GCGC 2 cut(s) 97, 433
AspS9I GGNCC 1 cut(s) 228
AsuC2I CCSGG 3 cut(s) 189, 190, 435
AsuHPI GGTGA 1 cut(s) 123
AvaI CYCGRG 1 cut(s) 188
BaeI ACNNNNGTAYC 4 cut(s) 285, 285, 318, 318
BbvI GCAGC 3 cut(s) 84, 415, 464
BccI CCATC 3 cut(s) 78, 98, 155
BcnI CCSGG 3 cut(s) 189, 190, 435
BfaI CTAG 1 cut(s) 527
BfmI CTRYAG 2 cut(s) 441, 450
BfoI RGCGCY 1 cut(s) 98
BisI GCNGC 4 cut(s) 98, 384, 429, 453
BlsI GCNGC 4 cut(s) 99, 385, 430, 454
Bme1390I CCNGG 3 cut(s) 189, 190, 435
BmeT110I CYCGRG 1 cut(s) 188
BmgT120I GGNCC 1 cut(s) 228
BmrFI CCNGG 3 cut(s) 189, 190, 435
BmrI ACTGGG 1 cut(s) 235
BmsI GCATC 1 cut(s) 56
BmuI ACTGGG 1 cut(s) 235
BpuMI CCSGG 3 cut(s) 189, 190, 435
BsaJI CCNNGG 2 cut(s) 188, 189
Bsc4I CCNNNNNNNGG 4 cut(s) 140, 189, 190, 410
Bse1I ACTGG 1 cut(s) 230
BseDI CCNNGG 2 cut(s) 188, 189
BseGI GGATG 1 cut(s) 71
BseLI CCNNNNNNNGG 4 cut(s) 140, 189, 190, 410
BseNI ACTGG 1 cut(s) 230
BseXI GCAGC 3 cut(s) 84, 415, 464
BseYI CCCAGC 1 cut(s) 479
BshFI GGCC 1 cut(s) 230
BsiHKCI CYCGRG 1 cut(s) 188
BsiSI CCGG 2 cut(s) 189, 435
BslI CCNNNNNNNGG 4 cut(s) 140, 189, 190, 410
BsmI GAATGC 1 cut(s) 220
BsnI GGCC 1 cut(s) 230
BsoBI CYCGRG 1 cut(s) 188
Bsp143I GATC 1 cut(s) 437
BspACI CCGC 2 cut(s) 134, 384
BspANI GGCC 1 cut(s) 230
BspMAI CTGCAG 1 cut(s) 454
BspPI GGATC 1 cut(s) 445
BsrI ACTGG 1 cut(s) 230
BssECI CCNNGG 2 cut(s) 188, 189
BssMI GATC 1 cut(s) 437
BstC8I GCNNGC 3 cut(s) 257, 364, 505
BstF5I GGATG 1 cut(s) 71
BstH2I RGCGCY 1 cut(s) 98
BstHHI GCGC 2 cut(s) 97, 433
BstKTI GATC 1 cut(s) 440
BstMBI GATC 1 cut(s) 437
BstMWI GCNNNNNNNGC 1 cut(s) 317
BstSCI CCNGG 3 cut(s) 187, 188, 433
BstSFI CTRYAG 2 cut(s) 441, 450
BstV1I GCAGC 3 cut(s) 84, 415, 464
BstX2I RGATCY 1 cut(s) 437
BstYI RGATCY 1 cut(s) 437
BsuRI GGCC 1 cut(s) 230
BtsCI GGATG 1 cut(s) 71
BtsI GCAGTG 1 cut(s) 53
BtsIMutI CAGTG 1 cut(s) 53
Cac8I GCNNGC 3 cut(s) 257, 364, 505
CfoI GCGC 2 cut(s) 97, 433
Cfr13I GGNCC 1 cut(s) 228
Cfr9I CCCGGG 1 cut(s) 188
CseI GACGC 1 cut(s) 409
CspCI CAANNNNNGTGG 2 cut(s) 72, 107
CviAII CATG 5 cut(s) 16, 61, 171, 308, 410
CviJI RGCY 7 cut(s) 28, 182, 203, 230, 259, 447, 483
CviKI_1 RGCY 7 cut(s) 28, 182, 203, 230, 259, 447, 483
DpnI GATC 1 cut(s) 439
DpnII GATC 1 cut(s) 437
Eco88I CYCGRG 1 cut(s) 188
EcoRI GAATTC 1 cut(s) 349
EcoT22I ATGCAT 1 cut(s) 220
FaeI CATG 5 cut(s) 19, 64, 174, 311, 413
FaiI YATR 8 cut(s) 17, 31, 62, 172, 309, 318, 411, 426
FatI CATG 5 cut(s) 15, 60, 170, 307, 409
Fnu4HI GCNGC 4 cut(s) 98, 384, 429, 453
FokI GGATG 1 cut(s) 78
Fsp4HI GCNGC 4 cut(s) 98, 384, 429, 453
FspBI CTAG 1 cut(s) 527
GlaI GCGC 2 cut(s) 96, 432
GluI GCNGC 4 cut(s) 98, 384, 429, 453
GsaI CCCAGC 1 cut(s) 483
HaeII RGCGCY 1 cut(s) 98
HaeIII GGCC 1 cut(s) 230
HapII CCGG 2 cut(s) 189, 435
HgaI GACGC 1 cut(s) 409
HhaI GCGC 2 cut(s) 97, 433
Hin1II CATG 5 cut(s) 19, 64, 174, 311, 413
Hin6I GCGC 2 cut(s) 95, 431
HinP1I GCGC 2 cut(s) 95, 431
HpaII CCGG 2 cut(s) 189, 435
HphI GGTGA 1 cut(s) 123
Hpy188I TCNGA 1 cut(s) 355
HpyAV CCTTC 2 cut(s) 134, 194
HpyCH4V TGCA 7 cut(s) 46, 100, 211, 218, 255, 366, 452
HpyF10VI GCNNNNNNNGC 1 cut(s) 317
Hsp92II CATG 5 cut(s) 19, 64, 174, 311, 413
HspAI GCGC 2 cut(s) 95, 431
Kzo9I GATC 1 cut(s) 437
LmnI GCTCC 2 cut(s) 107, 275
Lsp1109I GCAGC 3 cut(s) 84, 415, 464
LweI GCATC 1 cut(s) 56
MaeI CTAG 1 cut(s) 527
MaeIII GTNAC 3 cut(s) 129, 281, 388
MalI GATC 1 cut(s) 439
MboI GATC 1 cut(s) 437
MflI RGATCY 1 cut(s) 437
MluCI AATT 4 cut(s) 261, 328, 349, 367
MnlI CCTC 2 cut(s) 322, 468
Mph1103I ATGCAT 1 cut(s) 220
MspA1I CMGCKG 1 cut(s) 386
MspI CCGG 2 cut(s) 189, 435
MspR9I CCNGG 3 cut(s) 189, 190, 435
Mva1269I GAATGC 1 cut(s) 220
MwoI GCNNNNNNNGC 1 cut(s) 317
NciI CCSGG 3 cut(s) 189, 190, 435
NdeII GATC 1 cut(s) 437
NlaIII CATG 5 cut(s) 19, 64, 174, 311, 413
NmuCI GTSAC 2 cut(s) 129, 388
NsiI ATGCAT 1 cut(s) 220
PctI GAATGC 1 cut(s) 220
PflMI CCANNNNNTGG 1 cut(s) 410
PkrI GCNGC 4 cut(s) 99, 385, 430, 454
PspFI CCCAGC 1 cut(s) 479
PspPI GGNCC 1 cut(s) 228
PstI CTGCAG 1 cut(s) 454
PsuI RGATCY 1 cut(s) 437
SatI GCNGC 4 cut(s) 98, 384, 429, 453
Sau3AI GATC 1 cut(s) 437
Sau96I GGNCC 1 cut(s) 228
ScrFI CCNGG 3 cut(s) 189, 190, 435
SetI ASST 4 cut(s) 30, 274, 460, 485
SfaNI GCATC 1 cut(s) 56
SfcI CTRYAG 2 cut(s) 441, 450
SmaI CCCGGG 1 cut(s) 190
Sse9I AATT 4 cut(s) 261, 328, 349, 367
SsiI CCGC 2 cut(s) 134, 384
SspMI CTAG 1 cut(s) 527
StyD4I CCNGG 3 cut(s) 187, 188, 433
TasI AATT 4 cut(s) 261, 328, 349, 367
TauI GCSGC 1 cut(s) 386
TscAI CASTG 1 cut(s) 53
TseFI GTSAC 2 cut(s) 129, 388
TseI GCWGC 3 cut(s) 97, 428, 452
Tsp45I GTSAC 2 cut(s) 129, 388
TspDTI ATGAA 1 cut(s) 69
TspMI CCCGGG 1 cut(s) 188
TspRI CASTG 1 cut(s) 53
Van91I CCANNNNNTGG 1 cut(s) 410
XapI RAATTY 1 cut(s) 349
XmaI CCCGGG 1 cut(s) 188
XspI CTAG 1 cut(s) 527
Zsp2I ATGCAT 1 cut(s) 220
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.