RLG00000032194

glucan endo-1,3-beta-glucosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0005800
Physical Location & Seq
Forward (+)
11952504 .. 11958017
5514 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032194

Sequence Viewer

Length: 1218 bp
ATGACTGCAGATATGGGTGGATCATTCCAGAGCCCGAAACCATCATCAGGAGGATCATTTCAGGGCCTGAAACCATTGGCAGTTTCCGATGTGGACGACAAGTCTCTTGATAGCAAGGTCCATGAGGTTCCTGGCACCAAGGCGGAAGAATCAAAGCCTCTCCACGGTGCTGATTCTTGTTTTGATATCTCTAACAACCCCAGTAATGGCATCTATTTCTCCGACTGTGAGGATGACTCGAGTGATGAAGATGGGACTCGAGTGATGAAGATGAAGTGCTGCCTGTTGTTCATAGGATGCTATCTTGCTTTGACAATGGGAGTTGGTGTGGAAGAGAAAGTAGATGGTGCAATACCAGTGACCACATTGTCACCACCAGAAGGGAACACAACATTCCTTGATGGCACAACATGGTGTGTGGCTCTTGCTGGTGTCCCCCAAGATGATCTGCAGAATGCATTAGATTGGGCCTGTGGGCTTGGAATGGCAGACTGCAAGGCCATCCAAGAAGGCGGGCCCTGTTACGATCCAGACACTCTCTTATCTCATGCTTCCTTCGCTTTCAATAGTTACTATCAACAGAATGGGAATTCTGATATTGCTTGCAATTTTGGGGGCACTGCTGCTGTCACTAAACATAACCCAAGTTATGGAAAGTGTGTCTATGCTGCTCCTGGATCTGTAGGCTCTGCAGCACCTCCATTGTTGAGCTTTATTTGGTGGTGGAAACTTGCTCTCCTTTGTTTACTGCTTTTACTCCAGACCACCAGCTTCTCGTCTCCGTCGTCTGCCTTTTTCAACAACCACCAGGATCACCATAGCCACTACCACCACACCGACTACCACCACCACCGTTCCGCTTCATCGACGCATATCAATCTCTACACCTCCAGTGCTTTGTCTCTAGCTTTCCGCTTCTCCATCGAGCACCGTTCCATCTCCCCAAACCGATCCATCACCACTTCCAAGAAGAACGGCCCGATTGCGGCGGCCAGGAAGACGTGTATGTGCTTGCCGACGAATCATCCAAGCTCGTTTCGCTGCAGATCTGCCATGATGAACTCGCTGGTTCGAATCAGTGGAGTCGAGGGGGAGTGGGTGAAGAGAGCCTTCACGGCTCTATTCGTCCTTCTACGCACCAGCAGAGGCAGAGAACCGGGTTTCTCTATTTTTCTGATCTTAGATTCTGGCGAGTCAACTCCAAGTAGCCACGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
X8

Protein Analysis

406

Amino Acids

43.72

Weight (kDa)

5.99

Isoelectric Point (pI)

44.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
X8 PF07983 137 - 208 7.7e-23 X8 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 367
AccB1I GGYRCC 1 cut(s) 134
AccB7I CCANNNNNTGG 1 cut(s) 650
AciI CCGC 6 cut(s) 143, 513, 858, 913, 986, 989
AclWI GGATC 6 cut(s) 28, 61, 521, 685, 819, 945
AcoI YGGCCR 1 cut(s) 990
AcsI RAATTY 1 cut(s) 589
AfiI CCNNNNNNNGG 6 cut(s) 47, 164, 206, 380, 650, 985
AflIII ACRYGT 1 cut(s) 1001
AgsI TTSAA 2 cut(s) 565, 799
AhdI GACNNNNNGTC 1 cut(s) 100
AjiI CACGTC 1 cut(s) 1002
AjnI CCWGG 4 cut(s) 130, 673, 807, 992
AluBI AGCT 4 cut(s) 711, 771, 908, 1032
AluI AGCT 4 cut(s) 711, 771, 908, 1032
Alw21I GWGCWC 1 cut(s) 930
Alw26I GTCTC 3 cut(s) 108, 783, 906
AlwI GGATC 6 cut(s) 28, 61, 521, 685, 819, 945
AlwNI CAGNNNCTG 1 cut(s) 67
Ama87I CYCGRG 2 cut(s) 238, 258
AoxI GGCC 6 cut(s) 64, 468, 498, 515, 976, 990
ApaI GGGCCC 1 cut(s) 519
ApeKI GCWGC 5 cut(s) 279, 623, 668, 692, 1041
ApoI RAATTY 1 cut(s) 589
AspS9I GGNCC 6 cut(s) 64, 118, 468, 515, 516, 977
AsuC2I CCSGG 1 cut(s) 1158
AsuHPI GGTGA 4 cut(s) 363, 806, 949, 1111
AsuII TTCGAA 1 cut(s) 1072
AvaI CYCGRG 2 cut(s) 238, 258
AvaII GGWCC 1 cut(s) 118
BaeGI GKGCMC 2 cut(s) 519, 620
BanI GGYRCC 1 cut(s) 134
BanII GRGCYC 2 cut(s) 35, 519
BauI CACGAG 1 cut(s) 1211
BbsI GAAGAC 1 cut(s) 1004
Bbv12I GWGCWC 1 cut(s) 930
BbvI GCAGC 5 cut(s) 266, 610, 655, 704, 1028
BccI CCATC 8 cut(s) 49, 245, 338, 395, 509, 929, 944, 962
BceAI ACGGC 2 cut(s) 991, 1131
BciT130I CCWGG 4 cut(s) 132, 675, 809, 994
BcnI CCSGG 1 cut(s) 1158
BcoDI GTCTC 3 cut(s) 108, 783, 906
BfaI CTAG 1 cut(s) 905
BfmI CTRYAG 5 cut(s) 6, 449, 681, 690, 1042
BglI GCCNNNNNGGC 1 cut(s) 1115
BglII AGATCT 1 cut(s) 1046
BisI GCNGC 7 cut(s) 280, 624, 669, 693, 987, 990, 1042
BlsI GCNGC 7 cut(s) 281, 625, 670, 694, 988, 991, 1043
Bme1390I CCNGG 5 cut(s) 132, 675, 809, 994, 1158
Bme18I GGWCC 1 cut(s) 118
BmeRI GACNNNNNGTC 1 cut(s) 100
BmeT110I CYCGRG 2 cut(s) 238, 258
BmgBI CACGTC 1 cut(s) 1002
BmgT120I GGNCC 6 cut(s) 64, 118, 468, 515, 516, 977
BmiI GGNNCC 3 cut(s) 129, 136, 517
BmrFI CCNGG 5 cut(s) 132, 675, 809, 994, 1158
BmrI ACTGGG 1 cut(s) 195
BmsI GCATC 2 cut(s) 219, 287
BmuI ACTGGG 1 cut(s) 195
BpiI GAAGAC 1 cut(s) 1004
BplI GAGNNNNNCTC 2 cut(s) 221, 253
BpmI CTGGAG 2 cut(s) 743, 874
Bpu14I TTCGAA 1 cut(s) 1072
BpuMI CCSGG 1 cut(s) 1158
BsaJI CCNNGG 2 cut(s) 138, 163
BsaXI ACNNNNNCTCC 4 cut(s) 312, 342, 720, 750
Bsc4I CCNNNNNNNGG 6 cut(s) 47, 164, 206, 380, 650, 985
Bse1I ACTGG 3 cut(s) 201, 356, 891
BseBI CCWGG 4 cut(s) 132, 675, 809, 994
BseDI CCNNGG 2 cut(s) 138, 163
BseGI GGATG 4 cut(s) 238, 302, 501, 1024
BseLI CCNNNNNNNGG 6 cut(s) 47, 164, 206, 380, 650, 985
BseNI ACTGG 3 cut(s) 201, 356, 891
BseSI GKGCMC 2 cut(s) 519, 620
BseXI GCAGC 5 cut(s) 266, 610, 655, 704, 1028
BshFI GGCC 6 cut(s) 66, 470, 500, 517, 978, 992
BshNI GGYRCC 1 cut(s) 134
BsiHKAI GWGCWC 1 cut(s) 930
BsiHKCI CYCGRG 2 cut(s) 238, 258
BsiSI CCGG 1 cut(s) 1157
BslFI GGGAC 2 cut(s) 268, 419
BslI CCNNNNNNNGG 6 cut(s) 47, 164, 206, 380, 650, 985
BsmAI GTCTC 3 cut(s) 108, 783, 906
BsmBI CGTCTC 1 cut(s) 783
BsmFI GGGAC 2 cut(s) 268, 419
BsmI GAATGC 1 cut(s) 460
BsnI GGCC 6 cut(s) 66, 470, 500, 517, 978, 992
BsoBI CYCGRG 2 cut(s) 238, 258
Bsp119I TTCGAA 1 cut(s) 1072
Bsp120I GGGCCC 1 cut(s) 515
Bsp1286I GDGCHC 4 cut(s) 35, 519, 620, 930
Bsp143I GATC 9 cut(s) 20, 53, 445, 526, 677, 811, 950, 1046, 1176
BspACI CCGC 6 cut(s) 143, 513, 858, 913, 986, 989
BspANI GGCC 6 cut(s) 66, 470, 500, 517, 978, 992
BspLI GGNNCC 3 cut(s) 129, 136, 517
BspMAI CTGCAG 4 cut(s) 10, 453, 694, 1046
BspPI GGATC 6 cut(s) 28, 61, 521, 685, 819, 945
BspT104I TTCGAA 1 cut(s) 1072
BspT107I GGYRCC 1 cut(s) 134
BsrI ACTGG 3 cut(s) 201, 356, 891
BssECI CCNNGG 2 cut(s) 138, 163
BssMI GATC 9 cut(s) 20, 53, 445, 526, 677, 811, 950, 1046, 1176
BssSI CACGAG 1 cut(s) 1211
BssT1I CCWWGG 1 cut(s) 138
Bst2BI CACGAG 1 cut(s) 1211
Bst2UI CCWGG 4 cut(s) 132, 675, 809, 994
Bst4CI ACNGT 4 cut(s) 167, 227, 854, 932
Bst6I CTCTTC 2 cut(s) 327, 1097
BstBI TTCGAA 1 cut(s) 1072
BstC8I GCNNGC 3 cut(s) 515, 604, 1013
BstDEI CTNAG 1 cut(s) 1180
BstDSI CCRYGG 1 cut(s) 163
BstF5I GGATG 4 cut(s) 238, 302, 501, 1024
BstKTI GATC 9 cut(s) 23, 56, 448, 529, 680, 814, 953, 1049, 1179
BstMAI GTCTC 3 cut(s) 108, 783, 906
BstMBI GATC 9 cut(s) 20, 53, 445, 526, 677, 811, 950, 1046, 1176
BstMWI GCNNNNNNNGC 3 cut(s) 557, 1038, 1115
BstNI CCWGG 4 cut(s) 132, 675, 809, 994
BstSCI CCNGG 5 cut(s) 130, 673, 807, 992, 1156
BstSFI CTRYAG 5 cut(s) 6, 449, 681, 690, 1042
BstSLI GKGCMC 2 cut(s) 519, 620
BstV1I GCAGC 5 cut(s) 266, 610, 655, 704, 1028
BstV2I GAAGAC 1 cut(s) 1004
BstX2I RGATCY 2 cut(s) 677, 1046
BstYI RGATCY 2 cut(s) 677, 1046
BsuRI GGCC 6 cut(s) 66, 470, 500, 517, 978, 992
BtgI CCRYGG 1 cut(s) 163
BtrI CACGTC 1 cut(s) 1002
BtsCI GGATG 4 cut(s) 238, 302, 501, 1024
BtsI GCAGTG 1 cut(s) 618
BtsIMutI CAGTG 4 cut(s) 363, 618, 898, 1084
Cac8I GCNNGC 3 cut(s) 515, 604, 1013
CaiI CAGNNNCTG 1 cut(s) 67
Cfr13I GGNCC 6 cut(s) 64, 118, 468, 515, 516, 977
CseI GACGC 1 cut(s) 877
CviAII CATG 4 cut(s) 122, 411, 548, 1054
DdeI CTNAG 1 cut(s) 1180
DpnI GATC 9 cut(s) 22, 55, 447, 528, 679, 813, 952, 1048, 1178
DpnII GATC 9 cut(s) 20, 53, 445, 526, 677, 811, 950, 1046, 1176
DrdI GACNNNNNNGTC 1 cut(s) 367
DriI GACNNNNNGTC 1 cut(s) 100
DseDI GACNNNNNNGTC 1 cut(s) 367
EaeI YGGCCR 1 cut(s) 990
Eam1104I CTCTTC 2 cut(s) 327, 1097
Eam1105I GACNNNNNGTC 1 cut(s) 100
EarI CTCTTC 2 cut(s) 327, 1097
EciI GGCGGA 1 cut(s) 158
Eco130I CCWWGG 1 cut(s) 138
Eco24I GRGCYC 2 cut(s) 35, 519
Eco32I GATATC 1 cut(s) 187
Eco47I GGWCC 1 cut(s) 118
Eco88I CYCGRG 2 cut(s) 238, 258
EcoO109I RGGNCCY 2 cut(s) 64, 516
EcoRI GAATTC 1 cut(s) 589
EcoRII CCWGG 4 cut(s) 130, 673, 807, 992
EcoRV GATATC 1 cut(s) 187
EcoT14I CCWWGG 1 cut(s) 138
EcoT22I ATGCAT 1 cut(s) 460
EcoT38I GRGCYC 2 cut(s) 35, 519
ErhI CCWWGG 1 cut(s) 138
Esp3I CGTCTC 1 cut(s) 783
FaeI CATG 4 cut(s) 125, 414, 551, 1057
FalI AAGNNNNNCTT 2 cut(s) 1094, 1126
FaqI GGGAC 2 cut(s) 268, 419
FatI CATG 4 cut(s) 121, 410, 547, 1053
FauI CCCGC 1 cut(s) 506
Fnu4HI GCNGC 7 cut(s) 280, 624, 669, 693, 987, 990, 1042
FokI GGATG 4 cut(s) 245, 309, 488, 1011
FriOI GRGCYC 2 cut(s) 35, 519
Fsp4HI GCNGC 7 cut(s) 280, 624, 669, 693, 987, 990, 1042
FspBI CTAG 1 cut(s) 905
GluI GCNGC 7 cut(s) 280, 624, 669, 693, 987, 990, 1042
GsuI CTGGAG 2 cut(s) 743, 874
HaeIII GGCC 6 cut(s) 66, 470, 500, 517, 978, 992
HapII CCGG 1 cut(s) 1157
HgaI GACGC 1 cut(s) 877
Hin1II CATG 4 cut(s) 125, 414, 551, 1057
HincII GTYRAC 1 cut(s) 1197
HindII GTYRAC 1 cut(s) 1197
HinfI GANTC 9 cut(s) 149, 173, 236, 256, 1021, 1074, 1083, 1184, 1193
HpaII CCGG 1 cut(s) 1157
HphI GGTGA 4 cut(s) 363, 806, 949, 1111
Hpy166II GTNNAC 3 cut(s) 94, 746, 1197
Hpy188I TCNGA 4 cut(s) 88, 223, 595, 1176
Hpy188III TCNNGA 5 cut(s) 28, 48, 107, 530, 760
Hpy8I GTNNAC 3 cut(s) 94, 746, 1197
Hpy99I CGWCG 3 cut(s) 787, 871, 1021
HpyAV CCTTC 5 cut(s) 374, 503, 565, 1120, 1139
HpyCH4III ACNGT 4 cut(s) 167, 227, 854, 932
HpyCH4IV ACGT 1 cut(s) 1001
HpyCH4V TGCA 8 cut(s) 8, 350, 451, 458, 495, 606, 692, 1044
HpyF10VI GCNNNNNNNGC 3 cut(s) 557, 1038, 1115
HpyF3I CTNAG 1 cut(s) 1180
HpySE526I ACGT 1 cut(s) 1001
Hsp92II CATG 4 cut(s) 125, 414, 551, 1057
Kzo9I GATC 9 cut(s) 20, 53, 445, 526, 677, 811, 950, 1046, 1176
LmnI GCTCC 1 cut(s) 676
Lsp1109I GCAGC 5 cut(s) 266, 610, 655, 704, 1028
LweI GCATC 2 cut(s) 219, 287
MaeI CTAG 1 cut(s) 905
MaeII ACGT 1 cut(s) 1001
MaeIII GTNAC 5 cut(s) 358, 369, 521, 569, 628
MalI GATC 9 cut(s) 22, 55, 447, 528, 679, 813, 952, 1048, 1178
MboI GATC 9 cut(s) 20, 53, 445, 526, 677, 811, 950, 1046, 1176
MboII GAAGA 7 cut(s) 158, 260, 280, 344, 982, 1009, 1114
MflI RGATCY 2 cut(s) 677, 1046
MhlI GDGCHC 4 cut(s) 35, 519, 620, 930
MluCI AATT 2 cut(s) 589, 607
MlyI GAGTC 4 cut(s) 230, 250, 1092, 1202
MmeI TCCRAC 1 cut(s) 246
MnlI CCTC 8 cut(s) 44, 118, 168, 223, 708, 898, 1081, 1139
Mph1103I ATGCAT 1 cut(s) 460
MspI CCGG 1 cut(s) 1157
MspR9I CCNGG 5 cut(s) 132, 675, 809, 994, 1158
Mva1269I GAATGC 1 cut(s) 460
MvaI CCWGG 4 cut(s) 132, 675, 809, 994
MwoI GCNNNNNNNGC 3 cut(s) 557, 1038, 1115
NciI CCSGG 1 cut(s) 1158
NdeII GATC 9 cut(s) 20, 53, 445, 526, 677, 811, 950, 1046, 1176
NlaIII CATG 4 cut(s) 125, 414, 551, 1057
NlaIV GGNNCC 3 cut(s) 129, 136, 517
NmuCI GTSAC 3 cut(s) 358, 369, 628
NsiI ATGCAT 1 cut(s) 460
NspV TTCGAA 1 cut(s) 1072
PaeR7I CTCGAG 2 cut(s) 238, 258
PcsI WCGNNNNNNNCGW 1 cut(s) 782
PctI GAATGC 1 cut(s) 460
PfeI GAWTC 5 cut(s) 149, 173, 1021, 1074, 1184
PflMI CCANNNNNTGG 1 cut(s) 650
PfoI TCCNGGA 1 cut(s) 673
PkrI GCNGC 7 cut(s) 281, 625, 670, 694, 988, 991, 1043
PleI GAGTC 4 cut(s) 230, 250, 1091, 1201
PpsI GAGTC 4 cut(s) 230, 250, 1091, 1201
Psp6I CCWGG 4 cut(s) 130, 673, 807, 992
PspGI CCWGG 4 cut(s) 130, 673, 807, 992
PspN4I GGNNCC 3 cut(s) 129, 136, 517
PspOMI GGGCCC 1 cut(s) 515
PspPI GGNCC 6 cut(s) 64, 118, 468, 515, 516, 977
PspXI VCTCGAGB 2 cut(s) 238, 258
PstI CTGCAG 4 cut(s) 10, 453, 694, 1046
PstNI CAGNNNCTG 1 cut(s) 67
PsuI RGATCY 2 cut(s) 677, 1046
SatI GCNGC 7 cut(s) 280, 624, 669, 693, 987, 990, 1042
Sau3AI GATC 9 cut(s) 20, 53, 445, 526, 677, 811, 950, 1046, 1176
Sau96I GGNCC 6 cut(s) 64, 118, 468, 515, 516, 977
SchI GAGTC 4 cut(s) 230, 250, 1092, 1202
ScrFI CCNGG 5 cut(s) 132, 675, 809, 994, 1158
SduI GDGCHC 4 cut(s) 35, 519, 620, 930
SetI ASST 9 cut(s) 120, 129, 700, 713, 773, 890, 910, 1004, 1034
SfaNI GCATC 2 cut(s) 219, 287
SfcI CTRYAG 5 cut(s) 6, 449, 681, 690, 1042
Sfr274I CTCGAG 2 cut(s) 238, 258
SfuI TTCGAA 1 cut(s) 1072
SinI GGWCC 1 cut(s) 118
SlaI CTCGAG 2 cut(s) 238, 258
SmlI CTYRAG 2 cut(s) 238, 258
SmoI CTYRAG 2 cut(s) 238, 258
Sse9I AATT 2 cut(s) 589, 607
SsiI CCGC 6 cut(s) 143, 513, 858, 913, 986, 989
SspMI CTAG 1 cut(s) 905
StyD4I CCNGG 5 cut(s) 130, 673, 807, 992, 1156
StyI CCWWGG 1 cut(s) 138
TaaI ACNGT 4 cut(s) 167, 227, 854, 932
TaiI ACGT 1 cut(s) 1004
TaqI TCGA 6 cut(s) 239, 259, 866, 924, 1072, 1086
TasI AATT 2 cut(s) 589, 607
TauI GCSGC 2 cut(s) 989, 992
TfiI GAWTC 5 cut(s) 149, 173, 1021, 1074, 1184
TscAI CASTG 4 cut(s) 363, 625, 898, 1084
TseFI GTSAC 3 cut(s) 358, 369, 628
TseI GCWGC 5 cut(s) 279, 623, 668, 692, 1041
Tsp45I GTSAC 3 cut(s) 358, 369, 628
TspDTI ATGAA 6 cut(s) 261, 280, 281, 287, 852, 1073
TspGWI ACGGA 1 cut(s) 771
TspRI CASTG 4 cut(s) 363, 625, 898, 1084
Van91I CCANNNNNTGG 1 cut(s) 650
VpaK11BI GGWCC 1 cut(s) 118
XapI RAATTY 1 cut(s) 589
XcmI CCANNNNNNNNNTGG 1 cut(s) 128
XhoI CTCGAG 2 cut(s) 238, 258
XspI CTAG 1 cut(s) 905
Zsp2I ATGCAT 1 cut(s) 460
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.