FvH4_3g19440

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0004305
Physical Location & Seq
Forward (+)
12606860 .. 12607408
549 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g19440.t1

Sequence Viewer

Length: 471 bp
ATGAAAGGTCTCGTCGGTCTCTTTAATCCCGTCGCTCTCACCTCCGACCAAGCCGCCTCCCTCAGCCACCACCCCGCCGTCCTCCATGTCTTCGAAGACCGCCGCCGCCACCTCCACACCACCCGCTCGCCGCAGTTCCTAGGGCTTCGAAACCAGCGCGGGCTCTGGTTCGAATCCGACTACAGCTCTGACGTCATCGTAGGGGTTTTCGACATCGGAGTCTGGCCGGAGCATCGGAGCATCTTGGATTTGAATCTCGGCCCGATGCCAAAGCGGTGGAGAGGCCATGAAGCCGCTGCCAATGCCAGTGGTCTGATGACGGCGATCAACGGCTCGGTGGAATTCCGGTCTGCAAGAGACGTCGTCGGAAGAAGGTCGGTTAAGAAAGAGGTAGGAGATTTTGAGTGGCTGGTCAACGAATTGAAGATATTGGTTGTGCAGTCAGAGAAATTGTTTGCATTTAGAGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

157

Amino Acids

17.57

Weight (kDa)

9.59

Isoelectric Point (pI)

52.98

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 218
AatII GACGTC 2 cut(s) 195, 363
AccBSI CCGCTC 1 cut(s) 126
AccII CGCG 1 cut(s) 159
AcoI YGGCCR 1 cut(s) 224
AcsI RAATTY 1 cut(s) 341
AcyI GRCGYC 2 cut(s) 192, 360
AgsI TTSAA 2 cut(s) 253, 424
AluBI AGCT 1 cut(s) 186
AluI AGCT 1 cut(s) 186
Alw26I GTCTC 3 cut(s) 14, 23, 351
AoxI GGCC 3 cut(s) 224, 259, 283
ApeKI GCWGC 1 cut(s) 296
ApoI RAATTY 1 cut(s) 341
AspA2I CCTAGG 1 cut(s) 139
AspLEI GCGC 1 cut(s) 159
AspS9I GGNCC 1 cut(s) 260
AsuHPI GGTGA 1 cut(s) 31
AsuII TTCGAA 3 cut(s) 93, 148, 171
AvrII CCTAGG 1 cut(s) 139
BanII GRGCYC 1 cut(s) 165
BbsI GAAGAC 2 cut(s) 82, 102
BbvCI CCTCAGC 1 cut(s) 62
BbvI GCAGC 1 cut(s) 283
BceAI ACGGC 3 cut(s) 62, 336, 346
BcoDI GTCTC 3 cut(s) 14, 23, 351
BfaI CTAG 1 cut(s) 140
BfmI CTRYAG 1 cut(s) 181
BisI GCNGC 6 cut(s) 54, 103, 106, 131, 294, 297
BlnI CCTAGG 1 cut(s) 139
BlsI GCNGC 6 cut(s) 55, 104, 107, 132, 295, 298
BmgT120I GGNCC 1 cut(s) 260
BmsI GCATC 3 cut(s) 241, 249, 255
BpiI GAAGAC 2 cut(s) 82, 102
Bpu10I CCTNAGC 1 cut(s) 62
Bpu14I TTCGAA 3 cut(s) 93, 148, 171
BsaBI GATNNNNATC 1 cut(s) 252
BsaHI GRCGYC 2 cut(s) 192, 360
BsaI GGTCTC 2 cut(s) 14, 23
BsaJI CCNNGG 1 cut(s) 139
BsaWI WCCGGW 1 cut(s) 345
Bse1I ACTGG 1 cut(s) 306
Bse8I GATNNNNATC 1 cut(s) 252
BseDI CCNNGG 1 cut(s) 139
BseJI GATNNNNATC 1 cut(s) 252
BseMII CTCAG 1 cut(s) 76
BseNI ACTGG 1 cut(s) 306
BseXI GCAGC 1 cut(s) 283
BsgI GTGCAG 1 cut(s) 458
Bsh1236I CGCG 1 cut(s) 159
BshFI GGCC 3 cut(s) 226, 261, 285
BsiSI CCGG 2 cut(s) 227, 346
BsmAI GTCTC 3 cut(s) 14, 23, 351
BsmBI CGTCTC 1 cut(s) 351
BsnI GGCC 3 cut(s) 226, 261, 285
Bso31I GGTCTC 2 cut(s) 14, 23
Bsp119I TTCGAA 3 cut(s) 93, 148, 171
Bsp1286I GDGCHC 1 cut(s) 165
Bsp143I GATC 1 cut(s) 324
BspANI GGCC 3 cut(s) 226, 261, 285
BspCNI CTCAG 1 cut(s) 75
BspFNI CGCG 1 cut(s) 159
BspT104I TTCGAA 3 cut(s) 93, 148, 171
BspTNI GGTCTC 2 cut(s) 14, 23
BsrBI CCGCTC 1 cut(s) 126
BsrI ACTGG 1 cut(s) 306
BssECI CCNNGG 1 cut(s) 139
BssMI GATC 1 cut(s) 324
BssNI GRCGYC 2 cut(s) 192, 360
BssT1I CCWWGG 1 cut(s) 139
BstACI GRCGYC 2 cut(s) 192, 360
BstBI TTCGAA 3 cut(s) 93, 148, 171
BstC8I GCNNGC 2 cut(s) 128, 161
BstDEI CTNAG 1 cut(s) 62
BstFNI CGCG 1 cut(s) 159
BstHHI GCGC 1 cut(s) 159
BstKTI GATC 1 cut(s) 327
BstMAI GTCTC 3 cut(s) 14, 23, 351
BstMBI GATC 1 cut(s) 324
BstMWI GCNNNNNNNGC 2 cut(s) 302, 464
BstSFI CTRYAG 1 cut(s) 181
BstUI CGCG 1 cut(s) 159
BstV1I GCAGC 1 cut(s) 283
BstV2I GAAGAC 2 cut(s) 82, 102
BstXI CCANNNNNNTGG 1 cut(s) 276
BsuRI GGCC 3 cut(s) 226, 261, 285
BtsIMutI CAGTG 1 cut(s) 313
Cac8I GCNNGC 2 cut(s) 128, 161
CfoI GCGC 1 cut(s) 159
Cfr13I GGNCC 1 cut(s) 260
CspCI CAANNNNNGTGG 2 cut(s) 289, 324
CviAII CATG 2 cut(s) 86, 287
DdeI CTNAG 1 cut(s) 62
DpnI GATC 1 cut(s) 326
DpnII GATC 1 cut(s) 324
DrdI GACNNNNNNGTC 1 cut(s) 218
DseDI GACNNNNNNGTC 1 cut(s) 218
EaeI YGGCCR 1 cut(s) 224
Eco130I CCWWGG 1 cut(s) 139
Eco24I GRGCYC 1 cut(s) 165
Eco31I GGTCTC 2 cut(s) 14, 23
EcoRI GAATTC 1 cut(s) 341
EcoT14I CCWWGG 1 cut(s) 139
EcoT38I GRGCYC 1 cut(s) 165
ErhI CCWWGG 1 cut(s) 139
Esp3I CGTCTC 1 cut(s) 351
FaeI CATG 2 cut(s) 89, 290
FaiI YATR 2 cut(s) 87, 288
FatI CATG 2 cut(s) 85, 286
FauI CCCGC 3 cut(s) 82, 131, 152
Fnu4HI GCNGC 6 cut(s) 54, 103, 106, 131, 294, 297
FriOI GRGCYC 1 cut(s) 165
Fsp4HI GCNGC 6 cut(s) 54, 103, 106, 131, 294, 297
FspBI CTAG 1 cut(s) 140
GlaI GCGC 1 cut(s) 158
GluI GCNGC 6 cut(s) 54, 103, 106, 131, 294, 297
HaeIII GGCC 3 cut(s) 226, 261, 285
HapII CCGG 2 cut(s) 227, 346
HhaI GCGC 1 cut(s) 159
Hin1I GRCGYC 2 cut(s) 192, 360
Hin1II CATG 2 cut(s) 89, 290
Hin6I GCGC 1 cut(s) 157
HinP1I GCGC 1 cut(s) 157
HincII GTYRAC 1 cut(s) 415
HindII GTYRAC 1 cut(s) 415
HinfI GANTC 3 cut(s) 173, 219, 253
HpaII CCGG 2 cut(s) 227, 346
HphI GGTGA 1 cut(s) 31
Hpy166II GTNNAC 1 cut(s) 415
Hpy188I TCNGA 8 cut(s) 46, 178, 190, 218, 237, 315, 368, 445
Hpy8I GTNNAC 1 cut(s) 415
Hpy99I CGWCG 4 cut(s) 17, 35, 365, 368
HpyAV CCTTC 1 cut(s) 366
HpyCH4IV ACGT 2 cut(s) 192, 360
HpyCH4V TGCA 3 cut(s) 353, 439, 458
HpyF10VI GCNNNNNNNGC 2 cut(s) 302, 464
HpyF3I CTNAG 1 cut(s) 62
HpySE526I ACGT 2 cut(s) 192, 360
Hsp92I GRCGYC 2 cut(s) 192, 360
Hsp92II CATG 2 cut(s) 89, 290
HspAI GCGC 1 cut(s) 157
Kzo9I GATC 1 cut(s) 324
LmnI GCTCC 2 cut(s) 229, 237
LpnPI CCDG 7 cut(s) 151, 167, 208, 240, 319, 359, 395
Lsp1109I GCAGC 1 cut(s) 283
LweI GCATC 3 cut(s) 241, 249, 255
MaeI CTAG 1 cut(s) 140
MaeII ACGT 2 cut(s) 192, 360
MalI GATC 1 cut(s) 326
MbiI CCGCTC 1 cut(s) 126
MboI GATC 1 cut(s) 324
MboII GAAGA 4 cut(s) 82, 107, 381, 436
MhlI GDGCHC 1 cut(s) 165
MluCI AATT 3 cut(s) 341, 419, 449
MlyI GAGTC 1 cut(s) 228
MmeI TCCRAC 3 cut(s) 69, 201, 346
MnlI CCTC 7 cut(s) 52, 67, 71, 92, 122, 275, 382
MseI TTAA 2 cut(s) 24, 381
MspA1I CMGCKG 1 cut(s) 296
MspI CCGG 2 cut(s) 227, 346
MvnI CGCG 1 cut(s) 159
MwoI GCNNNNNNNGC 2 cut(s) 302, 464
NdeII GATC 1 cut(s) 324
NlaIII CATG 2 cut(s) 89, 290
NmeAIII GCCGAG 1 cut(s) 237
NspV TTCGAA 3 cut(s) 93, 148, 171
PfeI GAWTC 2 cut(s) 173, 253
PflFI GACNNNGTC 1 cut(s) 362
PkrI GCNGC 6 cut(s) 55, 104, 107, 132, 295, 298
PleI GAGTC 1 cut(s) 227
PpsI GAGTC 1 cut(s) 227
PspPI GGNCC 1 cut(s) 260
PsyI GACNNNGTC 1 cut(s) 362
SaqAI TTAA 2 cut(s) 24, 381
SatI GCNGC 6 cut(s) 54, 103, 106, 131, 294, 297
Sau3AI GATC 1 cut(s) 324
Sau96I GGNCC 1 cut(s) 260
SchI GAGTC 1 cut(s) 228
SduI GDGCHC 1 cut(s) 165
SetI ASST 8 cut(s) 10, 44, 114, 188, 195, 363, 377, 393
SfaNI GCATC 3 cut(s) 241, 249, 255
SfcI CTRYAG 1 cut(s) 181
SfuI TTCGAA 3 cut(s) 93, 148, 171
Sse9I AATT 3 cut(s) 341, 419, 449
SspMI CTAG 1 cut(s) 140
StyI CCWWGG 1 cut(s) 139
TaiI ACGT 2 cut(s) 195, 363
TaqI TCGA 4 cut(s) 93, 148, 171, 210
TaqII GACCGA 1 cut(s) 5
TasI AATT 3 cut(s) 341, 419, 449
TauI GCSGC 5 cut(s) 56, 105, 108, 133, 296
TfiI GAWTC 2 cut(s) 173, 253
Tru1I TTAA 2 cut(s) 24, 381
Tru9I TTAA 2 cut(s) 24, 381
TscAI CASTG 1 cut(s) 313
TseI GCWGC 1 cut(s) 296
TspDTI ATGAA 2 cut(s) 17, 303
TspRI CASTG 1 cut(s) 313
Tth111I GACNNNGTC 1 cut(s) 362
XapI RAATTY 1 cut(s) 341
XmaJI CCTAGG 1 cut(s) 139
XspI CTAG 1 cut(s) 140
ZraI GACGTC 2 cut(s) 193, 361
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.