Rh2AG211900

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0004305
Physical Location & Seq
Reverse (-)
21552938 .. 21553300
363 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG211900.1

Sequence Viewer

Length: 363 bp
ATGTATGCATCCCATTACCACTGGTACACATTGGAGTTCGCCGACCCACCTCAAATCCTCCACGTATACGACACTGTTTTCCATGGCTTCTCTGCAACTCTCACCTCCGACCAAGTTGCCTCCCTCAACCAACACCCTGCCGTTCTCCACGTCTTCGAAGACCGCCGCCGCCAGCTCCACACCAACTGCTCGCCGCAGTTCCTGGGCCTGAGAAACCAGAAGGGGCTGTGGTCGGAATTGGACTACATCTCTGACGTCATCGTCGGGGTTTTCGATACTGGAGTTCGGCCGGAGCGTCGGAGCTTTTTCGATAGGAATCTCGGTCTAGTTCCAAAGCGGTGGAGAACAATTGGGCCTCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

120

Amino Acids

14.04

Weight (kDa)

8.0

Isoelectric Point (pI)

59.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 13 - 60 9.3e-13 Peptidase inhibitor I9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 260
AatII GACGTC 1 cut(s) 258
AccI GTMKAC 1 cut(s) 66
AciI CCGC 5 cut(s) 163, 166, 169, 194, 337
AcoI YGGCCR 1 cut(s) 287
AcyI GRCGYC 1 cut(s) 255
AfaI GTAC 1 cut(s) 26
AjiI CACGTC 1 cut(s) 151
AjnI CCWGG 1 cut(s) 201
AluBI AGCT 2 cut(s) 175, 303
AluI AGCT 2 cut(s) 175, 303
AlwNI CAGNNNCTG 1 cut(s) 202
AoxI GGCC 3 cut(s) 205, 287, 353
AspS9I GGNCC 2 cut(s) 205, 353
AsuHPI GGTGA 1 cut(s) 94
AsuII TTCGAA 1 cut(s) 156
BaeI ACNNNNGTAYC 2 cut(s) 267, 300
BbsI GAAGAC 2 cut(s) 145, 165
BceAI ACGGC 1 cut(s) 125
BcgI CGANNNNNNTGC 2 cut(s) 98, 132
BciT130I CCWGG 1 cut(s) 203
BfaI CTAG 1 cut(s) 326
BisI GCNGC 3 cut(s) 166, 169, 194
BlsI GCNGC 3 cut(s) 167, 170, 195
Bme1390I CCNGG 1 cut(s) 203
BmgBI CACGTC 1 cut(s) 151
BmgT120I GGNCC 2 cut(s) 205, 353
BmrFI CCNGG 1 cut(s) 203
BmsI GCATC 1 cut(s) 17
BpiI GAAGAC 2 cut(s) 145, 165
BpmI CTGGAG 1 cut(s) 300
Bpu14I TTCGAA 1 cut(s) 156
BsaAI YACGTR 1 cut(s) 64
BsaBI GATNNNNATC 1 cut(s) 315
BsaHI GRCGYC 1 cut(s) 255
BsaJI CCNNGG 2 cut(s) 82, 202
Bse1I ACTGG 2 cut(s) 26, 283
Bse8I GATNNNNATC 1 cut(s) 315
BseBI CCWGG 1 cut(s) 203
BseDI CCNNGG 2 cut(s) 82, 202
BseGI GGATG 1 cut(s) 8
BseJI GATNNNNATC 1 cut(s) 315
BseMII CTCAG 1 cut(s) 200
BseNI ACTGG 2 cut(s) 26, 283
BseX3I CGGCCG 1 cut(s) 287
Bsh1285I CGRYCG 1 cut(s) 290
BshFI GGCC 3 cut(s) 207, 289, 355
BsiEI CGRYCG 1 cut(s) 290
BsiSI CCGG 1 cut(s) 290
BsnI GGCC 3 cut(s) 207, 289, 355
Bsp119I TTCGAA 1 cut(s) 156
Bsp19I CCATGG 1 cut(s) 82
BspACI CCGC 5 cut(s) 163, 166, 169, 194, 337
BspANI GGCC 3 cut(s) 207, 289, 355
BspCNI CTCAG 1 cut(s) 201
BspT104I TTCGAA 1 cut(s) 156
BsrI ACTGG 2 cut(s) 26, 283
BssECI CCNNGG 2 cut(s) 82, 202
BssNAI GTATAC 1 cut(s) 67
BssNI GRCGYC 1 cut(s) 255
BssT1I CCWWGG 1 cut(s) 82
Bst1107I GTATAC 1 cut(s) 67
Bst2UI CCWGG 1 cut(s) 203
Bst4CI ACNGT 1 cut(s) 76
BstACI GRCGYC 1 cut(s) 255
BstBAI YACGTR 1 cut(s) 64
BstBI TTCGAA 1 cut(s) 156
BstC8I GCNNGC 2 cut(s) 173, 191
BstDEI CTNAG 1 cut(s) 209
BstDSI CCRYGG 1 cut(s) 82
BstF5I GGATG 1 cut(s) 8
BstMCI CGRYCG 1 cut(s) 290
BstNI CCWGG 1 cut(s) 203
BstSCI CCNGG 1 cut(s) 201
BstV2I GAAGAC 2 cut(s) 145, 165
BstXI CCANNNNNNTGG 1 cut(s) 339
BstZ17I GTATAC 1 cut(s) 67
BstZI CGGCCG 1 cut(s) 287
BsuRI GGCC 3 cut(s) 207, 289, 355
BtgI CCRYGG 1 cut(s) 82
BtrI CACGTC 1 cut(s) 151
BtsCI GGATG 1 cut(s) 8
BtsIMutI CAGTG 2 cut(s) 19, 72
Cac8I GCNNGC 2 cut(s) 173, 191
CaiI CAGNNNCTG 1 cut(s) 202
Cfr13I GGNCC 2 cut(s) 205, 353
CseI GACGC 1 cut(s) 284
Csp6I GTAC 1 cut(s) 25
CviAII CATG 1 cut(s) 83
CviJI RGCY 7 cut(s) 87, 175, 207, 226, 289, 303, 355
CviKI_1 RGCY 7 cut(s) 87, 175, 207, 226, 289, 303, 355
CviQI GTAC 1 cut(s) 25
DdeI CTNAG 1 cut(s) 209
DrdI GACNNNNNNGTC 1 cut(s) 260
DseDI GACNNNNNNGTC 1 cut(s) 260
EaeI YGGCCR 1 cut(s) 287
EagI CGGCCG 1 cut(s) 287
EclXI CGGCCG 1 cut(s) 287
Eco130I CCWWGG 1 cut(s) 82
Eco52I CGGCCG 1 cut(s) 287
EcoRII CCWGG 1 cut(s) 201
EcoT14I CCWWGG 1 cut(s) 82
EcoT22I ATGCAT 1 cut(s) 10
ErhI CCWWGG 1 cut(s) 82
FaeI CATG 1 cut(s) 86
FaiI YATR 3 cut(s) 6, 67, 84
FatI CATG 1 cut(s) 82
FblI GTMKAC 1 cut(s) 66
Fnu4HI GCNGC 3 cut(s) 166, 169, 194
Fsp4HI GCNGC 3 cut(s) 166, 169, 194
FspBI CTAG 1 cut(s) 326
GluI GCNGC 3 cut(s) 166, 169, 194
GsuI CTGGAG 1 cut(s) 300
HaeIII GGCC 3 cut(s) 207, 289, 355
HapII CCGG 1 cut(s) 290
HgaI GACGC 1 cut(s) 284
Hin1I GRCGYC 1 cut(s) 255
Hin1II CATG 1 cut(s) 86
HinfI GANTC 1 cut(s) 316
HpaII CCGG 1 cut(s) 290
HphI GGTGA 1 cut(s) 94
Hpy166II GTNNAC 2 cut(s) 27, 67
Hpy188I TCNGA 4 cut(s) 109, 235, 253, 300
Hpy8I GTNNAC 2 cut(s) 27, 67
Hpy99I CGWCG 2 cut(s) 266, 300
HpyAV CCTTC 1 cut(s) 214
HpyCH4III ACNGT 1 cut(s) 76
HpyCH4IV ACGT 3 cut(s) 63, 150, 255
HpyCH4V TGCA 2 cut(s) 8, 95
HpyF3I CTNAG 1 cut(s) 209
HpySE526I ACGT 3 cut(s) 63, 150, 255
Hsp92I GRCGYC 1 cut(s) 255
Hsp92II CATG 1 cut(s) 86
LmnI GCTCC 3 cut(s) 180, 292, 300
LpnPI CCDG 9 cut(s) 7, 150, 185, 188, 215, 221, 230, 264, 303
LweI GCATC 1 cut(s) 17
MaeI CTAG 1 cut(s) 326
MaeII ACGT 3 cut(s) 63, 150, 255
MboII GAAGA 2 cut(s) 145, 170
MfeI CAATTG 1 cut(s) 348
MluCI AATT 2 cut(s) 236, 348
MmeI TCCRAC 3 cut(s) 132, 213, 278
MnlI CCTC 5 cut(s) 60, 68, 115, 130, 134
Mph1103I ATGCAT 1 cut(s) 10
MspI CCGG 1 cut(s) 290
MspR9I CCNGG 1 cut(s) 203
MunI CAATTG 1 cut(s) 348
MvaI CCWGG 1 cut(s) 203
NcoI CCATGG 1 cut(s) 82
NlaIII CATG 1 cut(s) 86
NsiI ATGCAT 1 cut(s) 10
NspV TTCGAA 1 cut(s) 156
PcsI WCGNNNNNNNCGW 2 cut(s) 270, 292
PfeI GAWTC 1 cut(s) 316
PkrI GCNGC 3 cut(s) 167, 170, 195
Ppu21I YACGTR 1 cut(s) 64
Psp6I CCWGG 1 cut(s) 201
PspGI CCWGG 1 cut(s) 201
PspPI GGNCC 2 cut(s) 205, 353
PstNI CAGNNNCTG 1 cut(s) 202
RsaI GTAC 1 cut(s) 26
RsaNI GTAC 1 cut(s) 25
SatI GCNGC 3 cut(s) 166, 169, 194
Sau96I GGNCC 2 cut(s) 205, 353
ScrFI CCNGG 1 cut(s) 203
SetI ASST 7 cut(s) 52, 66, 107, 153, 177, 258, 305
SfaNI GCATC 1 cut(s) 17
SfuI TTCGAA 1 cut(s) 156
Sse9I AATT 2 cut(s) 236, 348
SsiI CCGC 5 cut(s) 163, 166, 169, 194, 337
SspMI CTAG 1 cut(s) 326
StyD4I CCNGG 1 cut(s) 201
StyI CCWWGG 1 cut(s) 82
TaaI ACNGT 1 cut(s) 76
TaiI ACGT 3 cut(s) 66, 153, 258
TaqI TCGA 4 cut(s) 156, 273, 309, 358
TaqII GACCGA 1 cut(s) 311
TasI AATT 2 cut(s) 236, 348
TauI GCSGC 3 cut(s) 168, 171, 196
TfiI GAWTC 1 cut(s) 316
TscAI CASTG 2 cut(s) 26, 79
TspRI CASTG 2 cut(s) 26, 79
XmiI GTMKAC 1 cut(s) 66
XspI CTAG 1 cut(s) 326
ZraI GACGTC 1 cut(s) 256
Zsp2I ATGCAT 1 cut(s) 10
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.