RchiOBHm_Chr6g0252351

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0004305
Physical Location & Seq
Reverse (-)
7528977 .. 7529633
657 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22626

Sequence Viewer

Length: 657 bp
ATGGCTGCCCCTCACGTGAGCGGAGCCGCCGCTCTGCTCAAGTCGGCTCACCCCGATTGGAGTCCGGCGGTGATAAGATCGGCGATGATGACGACTGCTGGCATCACCAATAATCAGAACAAGACCATGACTGATGAAGCTACTGGAAATCCCTCCACTCCTTATGATTTAGGGGCGGGTCATTTGAATTTGGATCGGGCCATGGACCCGGGTCTGGTATATGATATCACCGGTGAGGATTACGTCCGGTTTCTCTGCTCGGTCGGGTACGGGCCGAGAGTAATCCAGGTGATCACTCGGTCGCCGCCGAAATGTCCGGGGAAAAAGACTTCGCCGGGAAATCTGAACTACCCGTCAATCGCCGCGTTGTTTCCGACTTCCTCGGCGGGGCCGTCCAGCAAGACGTTTATTCGGACGGTGACGAATGTGGGCCAGCCCAATGCGGTATACAGGCCCATGATAGAGGCGCCTAAAGGAGTGAAGGTGACGGTGAAGCCGTCGAAGCTGGTGTTCACGGAGGCGGTGAAGAAGCGGAGCTACTTGGTGACGGTGGCGGTGGATAGAAGCAACATCGTGTTGGGTGAGTCGGGGGTGTGTTCGGGGCGCTATATTGGAGCGATGGGAAGCAGCATGTGGTTCGGAGCCCCATTGTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

218

Amino Acids

23.1

Weight (kDa)

9.59

Isoelectric Point (pI)

35.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 1 - 49 8e-10 Subtilase family
fn3_6 PF17766 114 - 188 1.8e-20 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 466
AccBSI CCGCTC 2 cut(s) 21, 32
AccI GTMKAC 1 cut(s) 447
AccII CGCG 1 cut(s) 365
AclWI GGATC 1 cut(s) 201
AcsI RAATTY 1 cut(s) 187
AcvI CACGTG 1 cut(s) 16
AcyI GRCGYC 1 cut(s) 467
AfaI GTAC 1 cut(s) 269
AfiI CCNNNNNNNGG 3 cut(s) 214, 387, 651
AgeI ACCGGT 1 cut(s) 230
AgsI TTSAA 1 cut(s) 187
AjnI CCWGG 1 cut(s) 285
AluBI AGCT 3 cut(s) 140, 505, 537
AluI AGCT 3 cut(s) 140, 505, 537
AlwI GGATC 1 cut(s) 201
Ama87I CYCGRG 1 cut(s) 208
AoxI GGCC 5 cut(s) 198, 272, 389, 430, 452
ApeKI GCWGC 2 cut(s) 5, 627
ApoI RAATTY 1 cut(s) 187
AsiGI ACCGGT 1 cut(s) 230
AspLEI GCGC 2 cut(s) 469, 606
AspS9I GGNCC 6 cut(s) 198, 205, 272, 389, 430, 453
AsuC2I CCSGG 4 cut(s) 209, 210, 318, 336
AvaI CYCGRG 1 cut(s) 208
AvaII GGWCC 1 cut(s) 205
BanI GGYRCC 1 cut(s) 466
BanII GRGCYC 1 cut(s) 646
BarI GAAGNNNNNNTAC 2 cut(s) 521, 553
BbrPI CACGTG 1 cut(s) 16
BbvI GCAGC 1 cut(s) 639
BccI CCATC 1 cut(s) 613
BceAI ACGGC 2 cut(s) 376, 481
BcgI CGANNNNNNTGC 2 cut(s) 619, 653
BciT130I CCWGG 1 cut(s) 287
BclI TGATCA 1 cut(s) 291
BcnI CCSGG 4 cut(s) 209, 210, 318, 336
BfoI RGCGCY 2 cut(s) 470, 607
BisI GCNGC 6 cut(s) 6, 27, 30, 305, 363, 628
BlsI GCNGC 6 cut(s) 7, 28, 31, 306, 364, 629
Bme1390I CCNGG 5 cut(s) 209, 210, 287, 318, 336
Bme18I GGWCC 1 cut(s) 205
BmeT110I CYCGRG 1 cut(s) 208
BmgT120I GGNCC 6 cut(s) 198, 205, 272, 389, 430, 453
BmiI GGNNCC 5 cut(s) 25, 207, 390, 468, 643
BmrFI CCNGG 5 cut(s) 209, 210, 287, 318, 336
BmsI GCATC 1 cut(s) 111
BoxI GACNNNNGTC 1 cut(s) 210
BpuEI CTTGAG 1 cut(s) 23
BpuMI CCSGG 4 cut(s) 209, 210, 318, 336
BsaAI YACGTR 1 cut(s) 16
BsaHI GRCGYC 1 cut(s) 467
BsaJI CCNNGG 4 cut(s) 201, 208, 317, 381
BsaWI WCCGGW 2 cut(s) 230, 246
BsaXI ACNNNNNCTCC 2 cut(s) 468, 498
Bsc4I CCNNNNNNNGG 3 cut(s) 214, 387, 651
Bse118I RCCGGY 1 cut(s) 230
Bse1I ACTGG 1 cut(s) 148
BseBI CCWGG 1 cut(s) 287
BseDI CCNNGG 4 cut(s) 201, 208, 317, 381
BseLI CCNNNNNNNGG 3 cut(s) 214, 387, 651
BseNI ACTGG 1 cut(s) 148
BseXI GCAGC 1 cut(s) 639
Bsh1236I CGCG 1 cut(s) 365
Bsh1285I CGRYCG 2 cut(s) 264, 302
BshFI GGCC 5 cut(s) 200, 274, 391, 432, 454
BshNI GGYRCC 1 cut(s) 466
BshTI ACCGGT 1 cut(s) 230
BsiEI CGRYCG 2 cut(s) 264, 302
BsiHKCI CYCGRG 1 cut(s) 208
BsiSI CCGG 6 cut(s) 65, 209, 231, 247, 317, 335
BslI CCNNNNNNNGG 3 cut(s) 214, 387, 651
BsnI GGCC 5 cut(s) 200, 274, 391, 432, 454
BsoBI CYCGRG 1 cut(s) 208
Bsp1286I GDGCHC 1 cut(s) 646
Bsp143I GATC 3 cut(s) 77, 193, 291
Bsp19I CCATGG 1 cut(s) 201
BspANI GGCC 5 cut(s) 200, 274, 391, 432, 454
BspFNI CGCG 1 cut(s) 365
BspLI GGNNCC 5 cut(s) 25, 207, 390, 468, 643
BspPI GGATC 1 cut(s) 201
BspT107I GGYRCC 1 cut(s) 466
BsrBI CCGCTC 2 cut(s) 21, 32
BsrFI RCCGGY 1 cut(s) 230
BsrI ACTGG 1 cut(s) 148
BssAI RCCGGY 1 cut(s) 230
BssECI CCNNGG 4 cut(s) 201, 208, 317, 381
BssMI GATC 3 cut(s) 77, 193, 291
BssNAI GTATAC 1 cut(s) 448
BssNI GRCGYC 1 cut(s) 467
BssT1I CCWWGG 1 cut(s) 201
Bst1107I GTATAC 1 cut(s) 448
Bst2UI CCWGG 1 cut(s) 287
Bst4CI ACNGT 3 cut(s) 418, 490, 550
BstACI GRCGYC 1 cut(s) 467
BstBAI YACGTR 1 cut(s) 16
BstC8I GCNNGC 2 cut(s) 100, 434
BstDSI CCRYGG 1 cut(s) 201
BstFNI CGCG 1 cut(s) 365
BstH2I RGCGCY 2 cut(s) 470, 607
BstHHI GCGC 2 cut(s) 469, 606
BstKTI GATC 3 cut(s) 80, 196, 294
BstMBI GATC 3 cut(s) 77, 193, 291
BstMCI CGRYCG 2 cut(s) 264, 302
BstMWI GCNNNNNNNGC 1 cut(s) 502
BstNI CCWGG 1 cut(s) 287
BstNSI RCATGY 1 cut(s) 634
BstPAI GACNNNNGTC 1 cut(s) 210
BstSCI CCNGG 5 cut(s) 207, 208, 285, 316, 334
BstUI CGCG 1 cut(s) 365
BstV1I GCAGC 1 cut(s) 639
BstZ17I GTATAC 1 cut(s) 448
BsuRI GGCC 5 cut(s) 200, 274, 391, 432, 454
BtgI CCRYGG 1 cut(s) 201
BtgZI GCGATG 2 cut(s) 98, 632
Cac8I GCNNGC 2 cut(s) 100, 434
CfoI GCGC 2 cut(s) 469, 606
Cfr10I RCCGGY 1 cut(s) 230
Cfr13I GGNCC 6 cut(s) 198, 205, 272, 389, 430, 453
Cfr9I CCCGGG 1 cut(s) 208
Csp6I GTAC 1 cut(s) 268
CspAI ACCGGT 1 cut(s) 230
CviAII CATG 4 cut(s) 127, 202, 457, 631
CviQI GTAC 1 cut(s) 268
DinI GGCGCC 1 cut(s) 468
DpnI GATC 3 cut(s) 79, 195, 293
DpnII GATC 3 cut(s) 77, 193, 291
Eco130I CCWWGG 1 cut(s) 201
Eco24I GRGCYC 1 cut(s) 646
Eco32I GATATC 1 cut(s) 226
Eco47I GGWCC 1 cut(s) 205
Eco72I CACGTG 1 cut(s) 16
Eco88I CYCGRG 1 cut(s) 208
EcoRII CCWGG 1 cut(s) 285
EcoRV GATATC 1 cut(s) 226
EcoT14I CCWWGG 1 cut(s) 201
EcoT38I GRGCYC 1 cut(s) 646
EgeI GGCGCC 1 cut(s) 468
EheI GGCGCC 1 cut(s) 468
ErhI CCWWGG 1 cut(s) 201
FaeI CATG 4 cut(s) 130, 205, 460, 634
FaiI YATR 9 cut(s) 128, 165, 203, 220, 222, 448, 458, 609, 632
FatI CATG 4 cut(s) 126, 201, 456, 630
FauI CCCGC 2 cut(s) 169, 379
FbaI TGATCA 1 cut(s) 291
FblI GTMKAC 1 cut(s) 447
Fnu4HI GCNGC 6 cut(s) 6, 27, 30, 305, 363, 628
FriOI GRGCYC 1 cut(s) 646
Fsp4HI GCNGC 6 cut(s) 6, 27, 30, 305, 363, 628
GlaI GCGC 2 cut(s) 468, 605
GluI GCNGC 6 cut(s) 6, 27, 30, 305, 363, 628
HaeII RGCGCY 2 cut(s) 470, 607
HaeIII GGCC 5 cut(s) 200, 274, 391, 432, 454
HapII CCGG 6 cut(s) 65, 209, 231, 247, 317, 335
HhaI GCGC 2 cut(s) 469, 606
Hin1I GRCGYC 1 cut(s) 467
Hin1II CATG 4 cut(s) 130, 205, 460, 634
Hin6I GCGC 2 cut(s) 467, 604
HinP1I GCGC 2 cut(s) 467, 604
HinfI GANTC 2 cut(s) 61, 584
HpaII CCGG 6 cut(s) 65, 209, 231, 247, 317, 335
Hpy166II GTNNAC 2 cut(s) 448, 513
Hpy188I TCNGA 5 cut(s) 117, 345, 375, 414, 641
Hpy8I GTNNAC 2 cut(s) 448, 513
Hpy99I CGWCG 1 cut(s) 502
HpyAV CCTTC 1 cut(s) 475
HpyCH4III ACNGT 3 cut(s) 418, 490, 550
HpyCH4IV ACGT 3 cut(s) 15, 243, 404
HpyF10VI GCNNNNNNNGC 1 cut(s) 502
HpySE526I ACGT 3 cut(s) 15, 243, 404
Hsp92I GRCGYC 1 cut(s) 467
Hsp92II CATG 4 cut(s) 130, 205, 460, 634
HspAI GCGC 2 cut(s) 467, 604
KasI GGCGCC 1 cut(s) 466
Ksp22I TGATCA 1 cut(s) 291
Kzo9I GATC 3 cut(s) 77, 193, 291
LmnI GCTCC 4 cut(s) 23, 534, 614, 641
Lsp1109I GCAGC 1 cut(s) 639
LweI GCATC 1 cut(s) 111
MaeII ACGT 3 cut(s) 15, 243, 404
MaeIII GTNAC 3 cut(s) 418, 484, 544
MalI GATC 3 cut(s) 79, 195, 293
MbiI CCGCTC 2 cut(s) 21, 32
MboI GATC 3 cut(s) 77, 193, 291
MboII GAAGA 1 cut(s) 538
MhlI GDGCHC 1 cut(s) 646
MluCI AATT 1 cut(s) 187
Mly113I GGCGCC 1 cut(s) 467
MlyI GAGTC 2 cut(s) 70, 593
MmeI TCCRAC 1 cut(s) 398
MnlI CCTC 6 cut(s) 21, 163, 229, 391, 457, 511
MslI CAYNNNNRTG 1 cut(s) 652
MspI CCGG 6 cut(s) 65, 209, 231, 247, 317, 335
MspR9I CCNGG 5 cut(s) 209, 210, 287, 318, 336
MvaI CCWGG 1 cut(s) 287
MvnI CGCG 1 cut(s) 365
MwoI GCNNNNNNNGC 1 cut(s) 502
NarI GGCGCC 1 cut(s) 467
NciI CCSGG 4 cut(s) 209, 210, 318, 336
NcoI CCATGG 1 cut(s) 201
NdeII GATC 3 cut(s) 77, 193, 291
NlaIII CATG 4 cut(s) 130, 205, 460, 634
NlaIV GGNNCC 5 cut(s) 25, 207, 390, 468, 643
NmeAIII GCCGAG 2 cut(s) 300, 362
NmuCI GTSAC 3 cut(s) 418, 484, 544
NspI RCATGY 1 cut(s) 634
PcsI WCGNNNNNNNCGW 2 cut(s) 389, 494
PinAI ACCGGT 1 cut(s) 230
PkrI GCNGC 6 cut(s) 7, 28, 31, 306, 364, 629
PleI GAGTC 2 cut(s) 69, 592
PluTI GGCGCC 1 cut(s) 470
PmaCI CACGTG 1 cut(s) 16
PmlI CACGTG 1 cut(s) 16
PpsI GAGTC 2 cut(s) 69, 592
Ppu21I YACGTR 1 cut(s) 16
PshAI GACNNNNGTC 1 cut(s) 210
Psp6I CCWGG 1 cut(s) 285
PspCI CACGTG 1 cut(s) 16
PspGI CCWGG 1 cut(s) 285
PspN4I GGNNCC 5 cut(s) 25, 207, 390, 468, 643
PspPI GGNCC 6 cut(s) 198, 205, 272, 389, 430, 453
RsaI GTAC 1 cut(s) 269
RsaNI GTAC 1 cut(s) 268
RseI CAYNNNNRTG 1 cut(s) 652
SatI GCNGC 6 cut(s) 6, 27, 30, 305, 363, 628
Sau3AI GATC 3 cut(s) 77, 193, 291
Sau96I GGNCC 6 cut(s) 198, 205, 272, 389, 430, 453
SchI GAGTC 2 cut(s) 70, 593
ScrFI CCNGG 5 cut(s) 209, 210, 287, 318, 336
SduI GDGCHC 1 cut(s) 646
SetI ASST 8 cut(s) 18, 142, 246, 291, 407, 486, 507, 539
SfaNI GCATC 1 cut(s) 111
SfoI GGCGCC 1 cut(s) 468
SgrAI CRCCGGYG 1 cut(s) 230
SinI GGWCC 1 cut(s) 205
SmaI CCCGGG 1 cut(s) 210
SmiMI CAYNNNNRTG 1 cut(s) 652
SmlI CTYRAG 1 cut(s) 38
SmoI CTYRAG 1 cut(s) 38
Sse9I AATT 1 cut(s) 187
SspDI GGCGCC 1 cut(s) 466
StyD4I CCNGG 5 cut(s) 207, 208, 285, 316, 334
StyI CCWWGG 1 cut(s) 201
TaaI ACNGT 3 cut(s) 418, 490, 550
TaiI ACGT 3 cut(s) 18, 246, 407
TaqI TCGA 1 cut(s) 500
TaqII GACCGA 2 cut(s) 250, 288
TasI AATT 1 cut(s) 187
TauI GCSGC 4 cut(s) 29, 32, 307, 365
TseFI GTSAC 3 cut(s) 418, 484, 544
TseI GCWGC 2 cut(s) 5, 627
Tsp45I GTSAC 3 cut(s) 418, 484, 544
TspDTI ATGAA 1 cut(s) 150
TspGWI ACGGA 1 cut(s) 530
TspMI CCCGGG 1 cut(s) 208
VpaK11BI GGWCC 1 cut(s) 205
XapI RAATTY 1 cut(s) 187
XceI RCATGY 1 cut(s) 634
XmaI CCCGGG 1 cut(s) 208
XmiI GTMKAC 1 cut(s) 447
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.