Rh3DG088300

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0004305
Physical Location & Seq
Forward (+)
6832570 .. 6832800
231 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG088300.1

Sequence Viewer

Length: 231 bp
ATGTCTGCATCCCATTACCACTGGTACACCTCGGAGTTCGTCGACCCACCTCAAATTCTCCACGTATACGACACCGTTTTCCATGGCTTCTCTGCAACTCTCACCTCCGACCAAGTTGCCTCCCTCAACCAACACCCTGCCGCTCTCCACGTCTTCGAAGACCGCCTCAGCCAGCTCCACACCACCCTCTCGCCGCAGTTCCTGGGCCTGAGAAACCAGAAGGGGCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

76

Amino Acids

8.64

Weight (kDa)

6.1

Isoelectric Point (pI)

41.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 4 - 60 9.2e-11 Peptidase inhibitor I9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 143
AccI GTMKAC 2 cut(s) 42, 66
AciI CCGC 3 cut(s) 141, 163, 194
AcsI RAATTY 1 cut(s) 54
AfaI GTAC 1 cut(s) 26
AjiI CACGTC 1 cut(s) 151
AjnI CCWGG 1 cut(s) 201
AluBI AGCT 1 cut(s) 175
AluI AGCT 1 cut(s) 175
AlwNI CAGNNNCTG 1 cut(s) 202
AoxI GGCC 1 cut(s) 205
ApoI RAATTY 1 cut(s) 54
AspS9I GGNCC 1 cut(s) 205
AsuHPI GGTGA 1 cut(s) 94
AsuII TTCGAA 1 cut(s) 156
BbsI GAAGAC 2 cut(s) 145, 165
BbvCI CCTCAGC 1 cut(s) 167
BcgI CGANNNNNNTGC 2 cut(s) 98, 132
BciT130I CCWGG 1 cut(s) 203
BisI GCNGC 2 cut(s) 141, 194
BlsI GCNGC 2 cut(s) 142, 195
Bme1390I CCNGG 1 cut(s) 203
BmgBI CACGTC 1 cut(s) 151
BmgT120I GGNCC 1 cut(s) 205
BmrFI CCNGG 1 cut(s) 203
BmsI GCATC 1 cut(s) 17
BpiI GAAGAC 2 cut(s) 145, 165
Bpu10I CCTNAGC 1 cut(s) 167
Bpu14I TTCGAA 1 cut(s) 156
BsaAI YACGTR 1 cut(s) 64
BsaJI CCNNGG 3 cut(s) 30, 82, 202
Bse1I ACTGG 1 cut(s) 26
BseBI CCWGG 1 cut(s) 203
BseDI CCNNGG 3 cut(s) 30, 82, 202
BseGI GGATG 1 cut(s) 8
BseMII CTCAG 2 cut(s) 181, 200
BseNI ACTGG 1 cut(s) 26
BshFI GGCC 1 cut(s) 207
BsnI GGCC 1 cut(s) 207
Bsp119I TTCGAA 1 cut(s) 156
Bsp19I CCATGG 1 cut(s) 82
BspACI CCGC 3 cut(s) 141, 163, 194
BspANI GGCC 1 cut(s) 207
BspCNI CTCAG 2 cut(s) 180, 201
BspT104I TTCGAA 1 cut(s) 156
BsrBI CCGCTC 1 cut(s) 143
BsrI ACTGG 1 cut(s) 26
BssECI CCNNGG 3 cut(s) 30, 82, 202
BssNAI GTATAC 1 cut(s) 67
BssT1I CCWWGG 1 cut(s) 82
Bst1107I GTATAC 1 cut(s) 67
Bst2UI CCWGG 1 cut(s) 203
Bst4CI ACNGT 1 cut(s) 76
BstBAI YACGTR 1 cut(s) 64
BstBI TTCGAA 1 cut(s) 156
BstC8I GCNNGC 1 cut(s) 173
BstDEI CTNAG 2 cut(s) 167, 209
BstDSI CCRYGG 1 cut(s) 82
BstF5I GGATG 1 cut(s) 8
BstNI CCWGG 1 cut(s) 203
BstSCI CCNGG 1 cut(s) 201
BstV2I GAAGAC 2 cut(s) 145, 165
BstZ17I GTATAC 1 cut(s) 67
BsuRI GGCC 1 cut(s) 207
BtgI CCRYGG 1 cut(s) 82
BtrI CACGTC 1 cut(s) 151
BtsCI GGATG 1 cut(s) 8
BtsIMutI CAGTG 1 cut(s) 19
Cac8I GCNNGC 1 cut(s) 173
CaiI CAGNNNCTG 1 cut(s) 202
Cfr13I GGNCC 1 cut(s) 205
Csp6I GTAC 1 cut(s) 25
CviAII CATG 1 cut(s) 83
CviJI RGCY 5 cut(s) 87, 171, 175, 207, 226
CviKI_1 RGCY 5 cut(s) 87, 171, 175, 207, 226
CviQI GTAC 1 cut(s) 25
DdeI CTNAG 2 cut(s) 167, 209
Eco130I CCWWGG 1 cut(s) 82
EcoRII CCWGG 1 cut(s) 201
EcoT14I CCWWGG 1 cut(s) 82
ErhI CCWWGG 1 cut(s) 82
FaeI CATG 1 cut(s) 86
FaiI YATR 2 cut(s) 67, 84
FatI CATG 1 cut(s) 82
FblI GTMKAC 2 cut(s) 42, 66
Fnu4HI GCNGC 2 cut(s) 141, 194
Fsp4HI GCNGC 2 cut(s) 141, 194
GluI GCNGC 2 cut(s) 141, 194
HaeIII GGCC 1 cut(s) 207
Hin1II CATG 1 cut(s) 86
HincII GTYRAC 1 cut(s) 43
HindII GTYRAC 1 cut(s) 43
HphI GGTGA 1 cut(s) 94
Hpy166II GTNNAC 3 cut(s) 27, 43, 67
Hpy188I TCNGA 2 cut(s) 34, 109
Hpy8I GTNNAC 3 cut(s) 27, 43, 67
Hpy99I CGWCG 1 cut(s) 44
HpyAV CCTTC 1 cut(s) 214
HpyCH4III ACNGT 1 cut(s) 76
HpyCH4IV ACGT 2 cut(s) 63, 150
HpyCH4V TGCA 2 cut(s) 8, 95
HpyF3I CTNAG 2 cut(s) 167, 209
HpySE526I ACGT 2 cut(s) 63, 150
Hsp92II CATG 1 cut(s) 86
LmnI GCTCC 1 cut(s) 180
LpnPI CCDG 6 cut(s) 7, 150, 185, 188, 215, 221
LweI GCATC 1 cut(s) 17
MaeII ACGT 2 cut(s) 63, 150
MbiI CCGCTC 1 cut(s) 143
MboII GAAGA 2 cut(s) 145, 170
MluCI AATT 1 cut(s) 54
MmeI TCCRAC 1 cut(s) 132
MnlI CCTC 7 cut(s) 40, 60, 115, 130, 134, 176, 197
MspR9I CCNGG 1 cut(s) 203
MvaI CCWGG 1 cut(s) 203
NcoI CCATGG 1 cut(s) 82
NlaIII CATG 1 cut(s) 86
NspV TTCGAA 1 cut(s) 156
PkrI GCNGC 2 cut(s) 142, 195
Ppu21I YACGTR 1 cut(s) 64
Psp6I CCWGG 1 cut(s) 201
PspGI CCWGG 1 cut(s) 201
PspPI GGNCC 1 cut(s) 205
PstNI CAGNNNCTG 1 cut(s) 202
RsaI GTAC 1 cut(s) 26
RsaNI GTAC 1 cut(s) 25
SalI GTCGAC 1 cut(s) 41
SatI GCNGC 2 cut(s) 141, 194
Sau96I GGNCC 1 cut(s) 205
ScrFI CCNGG 1 cut(s) 203
SetI ASST 6 cut(s) 32, 52, 66, 107, 153, 177
SfaNI GCATC 1 cut(s) 17
SfuI TTCGAA 1 cut(s) 156
Sse9I AATT 1 cut(s) 54
SsiI CCGC 3 cut(s) 141, 163, 194
StyD4I CCNGG 1 cut(s) 201
StyI CCWWGG 1 cut(s) 82
TaaI ACNGT 1 cut(s) 76
TaiI ACGT 2 cut(s) 66, 153
TaqI TCGA 2 cut(s) 42, 156
TasI AATT 1 cut(s) 54
TauI GCSGC 2 cut(s) 143, 196
TscAI CASTG 1 cut(s) 26
TspRI CASTG 1 cut(s) 26
XapI RAATTY 1 cut(s) 54
XmiI GTMKAC 2 cut(s) 42, 66
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.