Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr3

Orthogroup ID

OG0004305

Physical Location & Seq

Genomic Coordinates Forward (+)

65376356 .. 65378707

Gene Structure 2352 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000015390

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2352 bp

ATGGATACCTTTCTCTCCATTTCCCTCATCTTCATCTTCTTCTTCTTCTTCGTATCTCAGATTGAATCAGAGCCCCTCTTCGTATCTCAGATTGAATCAGAGCCCCTCGATCAGACGGTGAAGACCTTCATCTTCAGAATCGACAGATTCTCCAAGCCGTCGATTTTCCAGTCGCATTACCACTGGTACACCTCGGAGTTCGCCGACCCACCTCAAATTCTCCACGTATACGACACCGTCTTCCATGGCTTCTCTGCAACTCTCACCTCCGACCAAGTTGCCTCCCTCAGCCAACACCCTGCCGTCCTCCACGTGTTCGAAGACCGCTGCCGCCAGCTCCACACCACCCGCTCGCCGCAGTTCCTCGGCCTGAGAAACCAGAAGGGGCTCTGGTCCGAATCCGATTACGGCTCCGACGTCATCGTCGGGGTTTTCGACACTGGAGTTTGGCCGGAGCGTCGGAGCTTTTCCGATAGGAATCTCGGTCCGGTTCCGAAGCGGTGGAGAGGGGTCTGTGAAACTGGAGTTAAATTTACAGCTCGGAATTGCAATAAGAAGGTGATCGGAGCTCGGTTCTTCATCAAAGGCCATGAAGCCGCCGCCAATGCCGGCGGTCCGATAACTGCGATCAACGGTTCAGTTGAATTCCGGTCTCCTAGAGATGCCGACGGACATGGAACACACACGGCGTCCACCGCCGCCGGCCGGTACGCCTTCGAGGCCAGCATGTCCGGCTACGCTTCCGGAATCGCAAAGGGAGTTGCTCCTAAATCTCGCTTGGCTGTCTACAAGGTCTGCTGGAAGGACTCCGGCTGCTTCGACTCCGATATCCTAGCCGCCTTCGACGCCGCCGTCAACGACGGTGTTGACGTCATTTCAATCTCAATTGGAGGCGGCGATGGAGTTTCTTCTCCCTATTATCTCGATCCGATTGCGATCGGATCATTCGGCGCCGCTGCGCATGGAGTGTTTGTTTCGGCCTCCGCCGGGAACGACGGACCTAACGGAATGTCGGTGACGAACCTGGCGCCGTGGCTGACGACTGTCGGAGCAGGAACAATTGACCGGAACTTCCCGGCGGTGGTGGTCCTAGGCGACGGCCGGAGACTCTCCGGAGTTTCTTTATACGCCGGAGCTCCGCTGAAGGGGAAGATGTACCCGCTGGTCTATCCCGGAAAGTCAGGTATGCTCTCTACTTCACTGTGCATGGAGAACTCCCTGGACCCGCGTCAGGTGAGGGGCAAAATCGTCATTTGCGACAGGGGAAACAATCCCAGAGTGGCGAAAGGTATGGTGGTGAAGAAAGCAGGAGGTGTGGGGATGATTCTGGCTAACGGAATCTCCAACGGCGAAGGACTCGTCGGCGATGCTCACCTTCTCCCCACCGCAGCTGTCGGCGCCGACGAAGGTGACGCCGTCAAGGCTTATGTCTCCTCTACCAAGACTCCTTCAGCTACTATTGATTTCGAGGGGACTGTAATCGGAATCAAGCCGGCTCCTGTGGTTGCTTCGTTTTCGGGTAGAGGGCCAAACGGGTTGAACCCGGAGATTCTGAAGCCGGATCTGATCGCTCCGGGAGTCAACATCCTTGCAGCTTGGACCGACGCTGTGGGCCCAACCGGGTTGGAAACGGATAACCGGAAAACGGAGTTCAATATCCTCTCGGGAACATCAATGGCTTGCCCTCACGTGAGTGGAGCCGCCGCTCTGCTGAAGTCTGCTCACCCCGATTGGAGTCCGGCGGCGATAAGATCGGCGATGATGACGACTGCTGGCATCACCAACAACCAGAACAAGACCATGACTGATGAGGCTACTGGAGACCCCTCCACTCCTTATGATTTAGGGGCGGGTCATTTGAATTTGGAGCGGGCTATGGACCCGGGTCTGGTATATGATATCACCGGCGAGGATTACGTCCAGTTTCTCTGCTCGGTCGGGTACGGGCCGAGAGTAATCCAGGTGATCACTCGGTCGCCGCCCAAATGTCCGGGGAAAAAGACTTCGCCGGGAAATTTGAACTACCCGTCCATCGCGGCGTTGTTTCCGACTTCCGCGGCGGGGCTGTCCAGTAAGACGTTTATTCGGACGGTGACGAATGTGGGTCAGCCCAATGCGGTGTACAGGCCCATGATAGAGGCGCCGAAAGGAGTGAAGGTGACGGTGAAGCCCTCGAAGCTGGTGTTCACGGAGGCGGTGAAGAAGCGGAGCTACTTGGTGACGGTGGCGGTGGATAGGAGCAACATGGTGTTGGGTGAGTCGGGGGGTGTGTTTGGGTCGCTGTATTGGAGCGATGGGAAGCAACATGTGGTTCGGAGCCCCATTGTGGTGACCCAAATGGATCCCTTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I9 PA Peptidase_S8

Protein Analysis

784

Amino Acids

83.18

Weight (kDa)

8.35

Isoelectric Point (pI)

34.97

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I9	PF05922	51 - 114	7.3e-13	Peptidase inhibitor I9
Peptidase_S8	PF00082	137 - 602	7.2e-47	Subtilase family
PA	PF02225	386 - 472	2.1e-13	PA domain
fn3_6	PF17766	672 - 777	1.4e-27	Fibronectin type-III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004305)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G34980
fragaria_vesca	FvH4_2g01410 FvH4_3g19440
malus_domestica	MD05G1120100.v1.1 MD10G1123500.v1.1
prunus_persica	Prupe.8G164000_v2.0.a1
pyrus_communis	pycom05g11390 pycom10g10580
rosa_chinensis	RchiOBHm_Chr6g0244021 RchiOBHm_Chr6g0252351 RchiOBHm_Chr6g0252361
rosa_laevigata	RLG00000015390 RLG00000029339
rosa_multiflora	Rmu_co8423929.1_g000001 Rmu_sc0001251.1_g000002 Rmu_sc0006415.1_g000012 Rmu_sc0006711.1_g000006 Rmu_sc0007419.1_g000002
rosa_roxburghii	Rroxscaffold_6G00388820 Rroxscaffold_6G00388840
rosa_rugosa	Rorug05G0223500 Rorug05G0506700 Rorug05G0515900
rosa_samantha	Rh1CG388200 Rh2AG211900 Rh3DG088300 Rh5BG286100 Rh5DG294800 Rh6AG021200 Rh6CG013400 Rh6DG014700
rosa_wichuraiana	Rw6G001740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	422, 851
AatII	GACGTC	2 cut(s)	420, 873
Acc16I	TGCGCA	1 cut(s)	960
AccB1I	GGYRCC	4 cut(s)	950, 1027, 1397, 2140
AccBSI	CCGCTC	3 cut(s)	351, 1706, 1870
AccI	GTMKAC	2 cut(s)	228, 786
AccII	CGCG	3 cut(s)	1228, 2036, 2057
AccIII	TCCGGA	2 cut(s)	743, 1112
AclWI	GGATC	5 cut(s)	920, 949, 1569, 2336, 2349
AcoI	YGGCCR	3 cut(s)	449, 703, 1099
AcsI	RAATTY	5 cut(s)	216, 530, 644, 1861, 2014
AcuI	CTGAAG	5 cut(s)	118, 1163, 1434, 1574, 1733
AcvI	CACGTG	2 cut(s)	313, 1690
AcyI	GRCGYC	9 cut(s)	417, 689, 846, 870, 951, 1028, 1398, 1413, 2141
AfaI	GTAC	5 cut(s)	188, 710, 1157, 1943, 2123
AfiI	CCNNNNNNNGG	9 cut(s)	705, 987, 1081, 1145, 1231, 1645, 1888, 2061, 2326
AflIII	ACRYGT	2 cut(s)	312, 2305
AgsI	TTSAA	8 cut(s)	65, 95, 644, 879, 1540, 1654, 1861, 2020
AjnI	CCWGG	3 cut(s)	1023, 1218, 1959
AleI	CACNNNNGTG	1 cut(s)	1692
AloI	GAACNNNNNNTCC	2 cut(s)	2012, 2044
Alw21I	GWGCWC	2 cut(s)	571, 1138
Alw26I	GTCTC	4 cut(s)	657, 1099, 1435, 1815
AlwI	GGATC	5 cut(s)	920, 949, 1569, 2336, 2349
Ama87I	CYCGRG	2 cut(s)	1663, 1882
Aor13HI	TCCGGA	2 cut(s)	743, 1112
ApaI	GGGCCC	1 cut(s)	1616
ApeKI	GCWGC	5 cut(s)	327, 813, 956, 1388, 1592
ApoI	RAATTY	5 cut(s)	216, 530, 644, 1861, 2014
ArsI	GACNNNNNNTTYG	2 cut(s)	1344, 1376
Asp700I	GAANNNNTTC	1 cut(s)	125
AspA2I	CCTAGG	1 cut(s)	1090
AspLEI	GCGC	5 cut(s)	953, 961, 1030, 1400, 2143
AsuII	TTCGAA	1 cut(s)	318
AvaI	CYCGRG	2 cut(s)	1663, 1882
AvaII	GGWCC	8 cut(s)	393, 485, 614, 998, 1087, 1222, 1599, 1879
AvrII	CCTAGG	1 cut(s)	1090
BaeGI	GKGCMC	1 cut(s)	1616
BamHI	GGATCC	1 cut(s)	2341
BanI	GGYRCC	4 cut(s)	950, 1027, 1397, 2140
BanII	GRGCYC	7 cut(s)	75, 105, 390, 571, 1138, 1616, 2321
BarI	GAAGNNNNNNTAC	2 cut(s)	2195, 2227
BbrPI	CACGTG	2 cut(s)	313, 1690
BbsI	GAAGAC	3 cut(s)	128, 232, 327
Bbv12I	GWGCWC	2 cut(s)	571, 1138
BbvCI	CCTCAGC	1 cut(s)	287
BbvI	GCAGC	5 cut(s)	314, 800, 943, 1400, 1604
BccI	CCATC	3 cut(s)	893, 2039, 2288
BceAI	ACGGC	9 cut(s)	142, 287, 424, 702, 836, 1015, 1114, 1363, 1400
BcgI	CGANNNNNNTGC	4 cut(s)	260, 294, 914, 948
BciT130I	CCWGG	3 cut(s)	1025, 1220, 1961
BclI	TGATCA	1 cut(s)	1965
BcoDI	GTCTC	4 cut(s)	657, 1099, 1435, 1815
BfaI	CTAG	3 cut(s)	657, 833, 1091
BfoI	RGCGCY	4 cut(s)	954, 1031, 1401, 2144
BglI	GCCNNNNNGGC	2 cut(s)	719, 1421
BlnI	CCTAGG	1 cut(s)	1090
Bme18I	GGWCC	8 cut(s)	393, 485, 614, 998, 1087, 1222, 1599, 1879
BmeT110I	CYCGRG	2 cut(s)	1663, 1882
BmsI	GCATC	3 cut(s)	652, 1357, 1785
BoxI	GACNNNNGTC	3 cut(s)	1043, 1227, 1884
BpiI	GAAGAC	3 cut(s)	128, 232, 327
BpmI	CTGGAG	3 cut(s)	462, 543, 1839
Bpu10I	CCTNAGC	1 cut(s)	287
Bpu14I	TTCGAA	1 cut(s)	318
BsaAI	YACGTR	3 cut(s)	226, 313, 1690
BsaBI	GATNNNNATC	2 cut(s)	477, 935
BsaHI	GRCGYC	9 cut(s)	417, 689, 846, 870, 951, 1028, 1398, 1413, 2141
BsaI	GGTCTC	2 cut(s)	657, 1815
BsaJI	CCNNGG	9 cut(s)	192, 244, 364, 1031, 1090, 1218, 1882, 1991, 2055
BsaWI	WCCGGW	6 cut(s)	487, 648, 743, 1065, 1112, 1638
Bsc4I	CCNNNNNNNGG	9 cut(s)	705, 987, 1081, 1145, 1231, 1645, 1888, 2061, 2326
Bse118I	RCCGGY	5 cut(s)	608, 701, 705, 1492, 1904
Bse1I	ACTGG	7 cut(s)	169, 188, 445, 526, 1822, 1921, 2070
Bse8I	GATNNNNATC	2 cut(s)	477, 935
BseAI	TCCGGA	2 cut(s)	743, 1112
BseBI	CCWGG	3 cut(s)	1025, 1220, 1961
BseDI	CCNNGG	9 cut(s)	192, 244, 364, 1031, 1090, 1218, 1882, 1991, 2055
BseGI	GGATG	2 cut(s)	1326, 1584
BseJI	GATNNNNATC	2 cut(s)	477, 935
BseLI	CCNNNNNNNGG	9 cut(s)	705, 987, 1081, 1145, 1231, 1645, 1888, 2061, 2326
BseMII	CTCAG	4 cut(s)	71, 101, 301, 362
BseNI	ACTGG	7 cut(s)	169, 188, 445, 526, 1822, 1921, 2070
BseRI	GAGGAG	1 cut(s)	1423
BseSI	GKGCMC	1 cut(s)	1616
BseX3I	CGGCCG	2 cut(s)	703, 1099
BseXI	GCAGC	5 cut(s)	314, 800, 943, 1400, 1604
Bsh1236I	CGCG	3 cut(s)	1228, 2036, 2057
Bsh1285I	CGRYCG	5 cut(s)	706, 939, 1102, 1938, 1976
BshNI	GGYRCC	4 cut(s)	950, 1027, 1397, 2140
BsiEI	CGRYCG	5 cut(s)	706, 939, 1102, 1938, 1976
BsiHKAI	GWGCWC	2 cut(s)	571, 1138
BsiHKCI	CYCGRG	2 cut(s)	1663, 1882
BslFI	GGGAC	1 cut(s)	1486
BslI	CCNNNNNNNGG	9 cut(s)	705, 987, 1081, 1145, 1231, 1645, 1888, 2061, 2326
BsmAI	GTCTC	4 cut(s)	657, 1099, 1435, 1815
BsmFI	GGGAC	1 cut(s)	1486
Bso31I	GGTCTC	2 cut(s)	657, 1815
BsoBI	CYCGRG	2 cut(s)	1663, 1882
Bsp119I	TTCGAA	1 cut(s)	318
Bsp120I	GGGCCC	1 cut(s)	1612
Bsp1286I	GDGCHC	7 cut(s)	75, 105, 390, 571, 1138, 1616, 2321
Bsp13I	TCCGGA	2 cut(s)	743, 1112
Bsp1407I	TGTACA	1 cut(s)	2121
Bsp19I	CCATGG	1 cut(s)	244
BspCNI	CTCAG	4 cut(s)	70, 100, 300, 363
BspEI	TCCGGA	2 cut(s)	743, 1112
BspFNI	CGCG	3 cut(s)	1228, 2036, 2057
BspPI	GGATC	5 cut(s)	920, 949, 1569, 2336, 2349
BspT104I	TTCGAA	1 cut(s)	318
BspT107I	GGYRCC	4 cut(s)	950, 1027, 1397, 2140
BspTNI	GGTCTC	2 cut(s)	657, 1815
BsrBI	CCGCTC	3 cut(s)	351, 1706, 1870
BsrFI	RCCGGY	5 cut(s)	608, 701, 705, 1492, 1904
BsrGI	TGTACA	1 cut(s)	2121
BsrI	ACTGG	7 cut(s)	169, 188, 445, 526, 1822, 1921, 2070
BssAI	RCCGGY	5 cut(s)	608, 701, 705, 1492, 1904
BssECI	CCNNGG	9 cut(s)	192, 244, 364, 1031, 1090, 1218, 1882, 1991, 2055
BssNAI	GTATAC	1 cut(s)	229
BssNI	GRCGYC	9 cut(s)	417, 689, 846, 870, 951, 1028, 1398, 1413, 2141
BssT1I	CCWWGG	2 cut(s)	244, 1090
Bst1107I	GTATAC	1 cut(s)	229
Bst2UI	CCWGG	3 cut(s)	1025, 1220, 1961
Bst6I	CTCTTC	1 cut(s)	83
BstACI	GRCGYC	9 cut(s)	417, 689, 846, 870, 951, 1028, 1398, 1413, 2141
BstAUI	TGTACA	1 cut(s)	2121
BstBAI	YACGTR	3 cut(s)	226, 313, 1690
BstBI	TTCGAA	1 cut(s)	318
BstC8I	GCNNGC	9 cut(s)	335, 353, 610, 703, 724, 1494, 1681, 1774, 1872
BstDEI	CTNAG	4 cut(s)	57, 87, 287, 371
BstDSI	CCRYGG	3 cut(s)	244, 1031, 2055
BstEII	GGTNACC	1 cut(s)	2329
BstF5I	GGATG	2 cut(s)	1326, 1584
BstFNI	CGCG	3 cut(s)	1228, 2036, 2057
BstH2I	RGCGCY	4 cut(s)	954, 1031, 1401, 2144
BstHHI	GCGC	5 cut(s)	953, 961, 1030, 1400, 2143
BstMAI	GTCTC	4 cut(s)	657, 1099, 1435, 1815
BstMCI	CGRYCG	5 cut(s)	706, 939, 1102, 1938, 1976
BstMWI	GCNNNNNNNGC	8 cut(s)	605, 695, 719, 732, 845, 1397, 1421, 2176
BstNI	CCWGG	3 cut(s)	1025, 1220, 1961
BstNSI	RCATGY	2 cut(s)	730, 2309
BstPAI	GACNNNNGTC	3 cut(s)	1043, 1227, 1884
BstPI	GGTNACC	1 cut(s)	2329
BstSLI	GKGCMC	1 cut(s)	1616
BstUI	CGCG	3 cut(s)	1228, 2036, 2057
BstV1I	GCAGC	5 cut(s)	314, 800, 943, 1400, 1604
BstV2I	GAAGAC	3 cut(s)	128, 232, 327
BstX2I	RGATCY	2 cut(s)	1561, 2341
BstYI	RGATCY	2 cut(s)	1561, 2341
BstZ17I	GTATAC	1 cut(s)	229
BstZI	CGGCCG	2 cut(s)	703, 1099
BtgI	CCRYGG	3 cut(s)	244, 1031, 2055
BtgZI	GCGATG	5 cut(s)	912, 1380, 1772, 2017, 2307
BtsCI	GGATG	2 cut(s)	1326, 1584
BtsIMutI	CAGTG	3 cut(s)	181, 438, 1199
Cac8I	GCNNGC	9 cut(s)	335, 353, 610, 703, 724, 1494, 1681, 1774, 1872
CfoI	GCGC	5 cut(s)	953, 961, 1030, 1400, 2143
Cfr10I	RCCGGY	5 cut(s)	608, 701, 705, 1492, 1904
Cfr42I	CCGCGG	1 cut(s)	2058
Cfr9I	CCCGGG	1 cut(s)	1882
CpoI	CGGWCCG	2 cut(s)	485, 614
CseI	GACGC	6 cut(s)	446, 678, 854, 1217, 1421, 1613
Csp6I	GTAC	5 cut(s)	187, 709, 1156, 1942, 2122
CspI	CGGWCCG	2 cut(s)	485, 614
CviQI	GTAC	5 cut(s)	187, 709, 1156, 1942, 2122
DdeI	CTNAG	4 cut(s)	57, 87, 287, 371
DinI	GGCGCC	4 cut(s)	952, 1029, 1399, 2142
DrdI	GACNNNNNNGTC	2 cut(s)	422, 851
DseDI	GACNNNNNNGTC	2 cut(s)	422, 851
EaeI	YGGCCR	3 cut(s)	449, 703, 1099
EagI	CGGCCG	2 cut(s)	703, 1099
Eam1104I	CTCTTC	1 cut(s)	83
EarI	CTCTTC	1 cut(s)	83
EciI	GGCGGA	1 cut(s)	973
Ecl136II	GAGCTC	2 cut(s)	569, 1136
EclXI	CGGCCG	2 cut(s)	703, 1099
Eco130I	CCWWGG	2 cut(s)	244, 1090
Eco24I	GRGCYC	7 cut(s)	75, 105, 390, 571, 1138, 1616, 2321
Eco31I	GGTCTC	2 cut(s)	657, 1815
Eco32I	GATATC	2 cut(s)	829, 1900
Eco47I	GGWCC	8 cut(s)	393, 485, 614, 998, 1087, 1222, 1599, 1879
Eco52I	CGGCCG	2 cut(s)	703, 1099
Eco53kI	GAGCTC	2 cut(s)	569, 1136
Eco57I	CTGAAG	5 cut(s)	118, 1163, 1434, 1574, 1733
Eco72I	CACGTG	2 cut(s)	313, 1690
Eco88I	CYCGRG	2 cut(s)	1663, 1882
Eco91I	GGTNACC	1 cut(s)	2329
EcoICRI	GAGCTC	2 cut(s)	569, 1136
EcoO65I	GGTNACC	1 cut(s)	2329
EcoRI	GAATTC	1 cut(s)	644
EcoRII	CCWGG	3 cut(s)	1023, 1218, 1959
EcoRV	GATATC	2 cut(s)	829, 1900
EcoT14I	CCWWGG	2 cut(s)	244, 1090
EcoT38I	GRGCYC	7 cut(s)	75, 105, 390, 571, 1138, 1616, 2321
EgeI	GGCGCC	4 cut(s)	952, 1029, 1399, 2142
EheI	GGCGCC	4 cut(s)	952, 1029, 1399, 2142
ErhI	CCWWGG	2 cut(s)	244, 1090
FaqI	GGGAC	1 cut(s)	1486
FauI	CCCGC	6 cut(s)	356, 1167, 1233, 1843, 1863, 2053
FbaI	TGATCA	1 cut(s)	1965
FblI	GTMKAC	2 cut(s)	228, 786
FokI	GGATG	2 cut(s)	1333, 1571
FriOI	GRGCYC	7 cut(s)	75, 105, 390, 571, 1138, 1616, 2321
FspBI	CTAG	3 cut(s)	657, 833, 1091
FspI	TGCGCA	1 cut(s)	960
GlaI	GCGC	5 cut(s)	952, 960, 1029, 1399, 2142
GsuI	CTGGAG	3 cut(s)	462, 543, 1839
HaeII	RGCGCY	4 cut(s)	954, 1031, 1401, 2144
HgaI	GACGC	6 cut(s)	446, 678, 854, 1217, 1421, 1613
HhaI	GCGC	5 cut(s)	953, 961, 1030, 1400, 2143
Hin1I	GRCGYC	9 cut(s)	417, 689, 846, 870, 951, 1028, 1398, 1413, 2141
Hin6I	GCGC	5 cut(s)	951, 959, 1028, 1398, 2141
HinP1I	GCGC	5 cut(s)	951, 959, 1028, 1398, 2141
HincII	GTYRAC	3 cut(s)	856, 868, 1582
HindII	GTYRAC	3 cut(s)	856, 868, 1582
Hpy166II	GTNNAC	9 cut(s)	189, 229, 693, 787, 856, 868, 1582, 2122, 2187
Hpy188III	TCNNGA	4 cut(s)	744, 923, 1113, 1665
Hpy8I	GTNNAC	9 cut(s)	189, 229, 693, 787, 856, 868, 1582, 2122, 2187
HpyCH4IV	ACGT	7 cut(s)	225, 312, 417, 870, 1689, 1917, 2078
HpyCH4V	TGCA	4 cut(s)	257, 549, 1206, 1592
HpyF10VI	GCNNNNNNNGC	8 cut(s)	605, 695, 719, 732, 845, 1397, 1421, 2176
HpyF3I	CTNAG	4 cut(s)	57, 87, 287, 371
HpySE526I	ACGT	7 cut(s)	225, 312, 417, 870, 1689, 1917, 2078
Hsp92I	GRCGYC	9 cut(s)	417, 689, 846, 870, 951, 1028, 1398, 1413, 2141
HspAI	GCGC	5 cut(s)	951, 959, 1028, 1398, 2141
KasI	GGCGCC	4 cut(s)	950, 1027, 1397, 2140
Kpn2I	TCCGGA	2 cut(s)	743, 1112
KroI	GCCGGC	3 cut(s)	608, 701, 1492
KroNI	GCCGGC	3 cut(s)	610, 703, 1494
Ksp22I	TGATCA	1 cut(s)	1965
KspI	CCGCGG	1 cut(s)	2058
Lsp1109I	GCAGC	5 cut(s)	314, 800, 943, 1400, 1604
LweI	GCATC	3 cut(s)	652, 1357, 1785
MaeI	CTAG	3 cut(s)	657, 833, 1091
MaeII	ACGT	7 cut(s)	225, 312, 417, 870, 1689, 1917, 2078
MaeIII	GTNAC	6 cut(s)	1015, 1409, 2092, 2158, 2218, 2329
MbiI	CCGCTC	3 cut(s)	351, 1706, 1870
MfeI	CAATTG	2 cut(s)	885, 1059
MflI	RGATCY	2 cut(s)	1561, 2341
MhlI	GDGCHC	7 cut(s)	75, 105, 390, 571, 1138, 1616, 2321
MluCI	AATT	8 cut(s)	216, 530, 544, 644, 885, 1059, 1861, 2014
Mly113I	GGCGCC	4 cut(s)	951, 1028, 1398, 2141
MlyI	GAGTC	8 cut(s)	800, 815, 1101, 1350, 1438, 1587, 1744, 2267
MmeI	TCCRAC	7 cut(s)	294, 438, 440, 1027, 1368, 1605, 2072
MroI	TCCGGA	2 cut(s)	743, 1112
MroNI	GCCGGC	3 cut(s)	608, 701, 1492
MroXI	GAANNNNTTC	1 cut(s)	125
MseI	TTAA	1 cut(s)	528
MslI	CAYNNNNRTG	2 cut(s)	1692, 2327
MspA1I	CMGCKG	6 cut(s)	327, 956, 1141, 1162, 1391, 2057
MunI	CAATTG	2 cut(s)	885, 1059
MvaI	CCWGG	3 cut(s)	1025, 1220, 1961
MvnI	CGCG	3 cut(s)	1228, 2036, 2057
MwoI	GCNNNNNNNGC	8 cut(s)	605, 695, 719, 732, 845, 1397, 1421, 2176
NaeI	GCCGGC	3 cut(s)	610, 703, 1494
NarI	GGCGCC	4 cut(s)	951, 1028, 1398, 2141
NcoI	CCATGG	1 cut(s)	244
NgoMIV	GCCGGC	3 cut(s)	608, 701, 1492
NmeAIII	GCCGAG	2 cut(s)	345, 1974
NmuCI	GTSAC	6 cut(s)	1015, 1409, 2092, 2158, 2218, 2329
NsbI	TGCGCA	1 cut(s)	960
NspI	RCATGY	2 cut(s)	730, 2309
NspV	TTCGAA	1 cut(s)	318
OliI	CACNNNNGTG	1 cut(s)	1692
PciI	ACATGT	1 cut(s)	2305
PcsI	WCGNNNNNNNCGW	6 cut(s)	414, 432, 849, 867, 1254, 1401
PdiI	GCCGGC	3 cut(s)	610, 703, 1494
PdmI	GAANNNNTTC	1 cut(s)	125
PflFI	GACNNNGTC	2 cut(s)	236, 1415
PfoI	TCCNGGA	2 cut(s)	1171, 1573
Ple19I	CGATCG	1 cut(s)	939
PleI	GAGTC	8 cut(s)	800, 815, 1101, 1350, 1438, 1586, 1743, 2266
PluTI	GGCGCC	4 cut(s)	954, 1031, 1401, 2144
PmaCI	CACGTG	2 cut(s)	313, 1690
PmlI	CACGTG	2 cut(s)	313, 1690
PpsI	GAGTC	8 cut(s)	800, 815, 1101, 1350, 1438, 1586, 1743, 2266
Ppu21I	YACGTR	3 cut(s)	226, 313, 1690
PscI	ACATGT	1 cut(s)	2305
PshAI	GACNNNNGTC	3 cut(s)	1043, 1227, 1884
Psp124BI	GAGCTC	2 cut(s)	571, 1138
Psp6I	CCWGG	3 cut(s)	1023, 1218, 1959
PspCI	CACGTG	2 cut(s)	313, 1690
PspEI	GGTNACC	1 cut(s)	2329
PspGI	CCWGG	3 cut(s)	1023, 1218, 1959
PspOMI	GGGCCC	1 cut(s)	1612
PsuI	RGATCY	2 cut(s)	1561, 2341
PsyI	GACNNNGTC	2 cut(s)	236, 1415
PvuI	CGATCG	1 cut(s)	939
PvuII	CAGCTG	1 cut(s)	1391
RsaI	GTAC	5 cut(s)	188, 710, 1157, 1943, 2123
RsaNI	GTAC	5 cut(s)	187, 709, 1156, 1942, 2122
RseI	CAYNNNNRTG	2 cut(s)	1692, 2327
Rsr2I	CGGWCCG	2 cut(s)	485, 614
RsrII	CGGWCCG	2 cut(s)	485, 614
SacI	GAGCTC	2 cut(s)	571, 1138
SacII	CCGCGG	1 cut(s)	2058
SaqAI	TTAA	1 cut(s)	528
SchI	GAGTC	8 cut(s)	800, 815, 1101, 1350, 1438, 1587, 1744, 2267
SduI	GDGCHC	7 cut(s)	75, 105, 390, 571, 1138, 1616, 2321
SfaNI	GCATC	3 cut(s)	652, 1357, 1785
SfoI	GGCGCC	4 cut(s)	952, 1029, 1399, 2142
Sfr303I	CCGCGG	1 cut(s)	2058
SfuI	TTCGAA	1 cut(s)	318
SgrAI	CRCCGGYG	1 cut(s)	1904
SgrBI	CCGCGG	1 cut(s)	2058
SinI	GGWCC	8 cut(s)	393, 485, 614, 998, 1087, 1222, 1599, 1879
SmaI	CCCGGG	1 cut(s)	1884
SmiMI	CAYNNNNRTG	2 cut(s)	1692, 2327
Sse9I	AATT	8 cut(s)	216, 530, 544, 644, 885, 1059, 1861, 2014
SspDI	GGCGCC	4 cut(s)	950, 1027, 1397, 2140
SspMI	CTAG	3 cut(s)	657, 833, 1091
SstI	GAGCTC	2 cut(s)	571, 1138
StyI	CCWWGG	2 cut(s)	244, 1090
TaiI	ACGT	7 cut(s)	228, 315, 420, 873, 1692, 1920, 2081
TaqII	GACCGA	4 cut(s)	473, 1616, 1924, 1962
TasI	AATT	8 cut(s)	216, 530, 544, 644, 885, 1059, 1861, 2014
TatI	WGTACW	1 cut(s)	2121
Tru1I	TTAA	1 cut(s)	528
Tru9I	TTAA	1 cut(s)	528
TscAI	CASTG	3 cut(s)	188, 445, 1206
TseFI	GTSAC	6 cut(s)	1015, 1409, 2092, 2158, 2218, 2329
TseI	GCWGC	5 cut(s)	327, 813, 956, 1388, 1592
Tsp45I	GTSAC	6 cut(s)	1015, 1409, 2092, 2158, 2218, 2329
TspDTI	ATGAA	4 cut(s)	22, 118, 568, 606
TspGWI	ACGGA	7 cut(s)	684, 1011, 1020, 1350, 1646, 1661, 2204
TspMI	CCCGGG	1 cut(s)	1882
TspRI	CASTG	3 cut(s)	188, 445, 1206
Tth111I	GACNNNGTC	2 cut(s)	236, 1415
VpaK11BI	GGWCC	8 cut(s)	393, 485, 614, 998, 1087, 1222, 1599, 1879
XapI	RAATTY	5 cut(s)	216, 530, 644, 1861, 2014
XceI	RCATGY	2 cut(s)	730, 2309
XmaI	CCCGGG	1 cut(s)	1882
XmaJI	CCTAGG	1 cut(s)	1090
XmiI	GTMKAC	2 cut(s)	228, 786
XmnI	GAANNNNTTC	1 cut(s)	125
XspI	CTAG	3 cut(s)	657, 833, 1091
ZraI	GACGTC	2 cut(s)	418, 871

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.