Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0004305

Physical Location & Seq

Genomic Coordinates Forward (+)

20089135 .. 20091450

Gene Structure 2316 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1123500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2316 bp

ATGGCCTCCAATCCTCTCCTCCCCCTCCTCTTTCTCCTCACATTATTCAAATTCTCAGCCCAAATCGCGCCGCAGGCCGCCGATCAAACCCTTAAGACCTTCATTTTCCGCGTTGACCGCCACTCCAAGCCCTCGATTTTCCCCACCCACTACCACTGGTACGCCTCCGAATTCGCCGACCCGCCCCAGATCCTCCACGTCTACGACACCGTTTTCCACGGCTTCTCCGCCTCTCTCACTCCCGACCAGGTCTCCGCCATCTCCGACCACCCTTCGGTGCTTGCTGTGATCCAGGACCGCCGCCGGAGTCTCCACACTACTCGATCCCCACAATTTTTGGGGCTCAGGAACCAGAGGGGGCTGTGGTCGGAATCGGACTATGGCTCCGACGTCATTGTCGGAGTATTTGACACCGGCGTCTGGCCGGAGCGTCGCAGTTTCACGGATAAGAATCTTGGGGCGATTCCGAGGCGGTGGAAGGGGGTTTGCGAGACAGGAACTAAATTTGCGAAGAGTAACTGCAACAGGAAGCTGATCGGAGCTCGATTCTTCATCAAGGGCCACGAGGCGGCGGCCAATGCTGGAGGTCCGATATCACCGATCAACGATACGGTGGAGTTCCGGTCGCCGAGAGATGCGGACGGTCATGGTACCCACACCGCCTCCACGGCGATCGGCCGGCACGCTTTCGAGGCGAGCATGTCGGGTTACGCCGCCGGAATAGCGAAAGGAGTCGCTCCAAAAGCTCGATTGGCAGTTTATAAGGTATGCTGGAAGGATTCTGGTTGCTTCGACTCTGATATATTGGCCGCCTTTGACGCCGCCGTTAATGACGGCGTTGATGTCATTTCTATTTCAATCGGAGGCGGCGACGGTGTTACTCCTCCGTATTATCTCGATCCGATTGCGATTGGATCGTACGGAGCGGTTTCGCACGGAGTGTTCGTGTCTTCCTCGGCCGGCAACGACGGTCCAAACGGCATGTCGGTGACTAATCTCGCGCCTTGGCTTACTACGGTGGGCGCGGGAACCATCGATCGGAATTTCCCGGCGGTTGTGATTTTAGGCAACGGCCGGAGACTAAGCGGTGTGTCGCTGTATTCCGGATTACCTCTCAAAGGCAAGATGTACCCTGTCGTTTATCCTGGGAAATCAGGCATGCTCTCTGCTTCGCTGTGCATGGAGAACTCTCTGGACCCGCGCCAGGTCAGGGGCAAAATTGTAATATGTGACCGCGGGAGCAGTCCTCGAGTGGCCAAAGGCTTAGTCGTGAAGAAAGCCGGCGGTGTTGGAATGATCCTCGCTAACGGAATTTCTAACGGCGAGGGTCTCGTCGGCGACGCACATATCCTGGCCACTTGCGCCGTCGGGGCCGACGAGGGTGACGCAATAAAGGCCTACGTGGCCTCCACCAAGACCCCCATGGCGACGCTCGATTTCCAAGGGACTGTGATTGGAATCAAACCGGCTCCGGTTGTGGCTTCGTTCTCGGGTCGTGGGCCGAACGGGCTGAGCCCGGAGATTCTGAAGCCAGACCTGATCGCCCCTGGAGTCAACATCTTGGCCGCTTGGACCGACGCCGTGGGACCCACCGGTCTGGACACTGACAAGAGGAAAACCGAGTTTAACATTTTGTCCGGGACTTCCATGGCCGCTCCGCACGTGAGCGGAGCCGCGGCTTTACTGAAGTCGGCTCACCGGGATTGGAGCCCGGCCGCCATAAGATCCGCCATGATGACAACGGCGAGCGTGACTGATAACAGAAATCAGACCATGACCGATGAGGCAACCGGAAAGCCCTCCACGCCTTATGATTTGGGGGCGGGTCATTTGAATTTGGGCCGGGCTATGGATCCTGGGCTTATTTATGACATCACCAATGATGATTACGTTCGGTTTCTTTGTGGAGTTGGGTATGGGCCAAGAGTGATTCAGGTCATCACTCGGGTCCAACCCAACTGTCCGGTGAAGAAGCCTGCGCCGGAGAATCTCAATTACCCGTCCATAGGCGCGGTGTTTTCCAGGGCGGGAAAGTCGAGTAAGATGTTTATCCGGAGCGTGACGAATGTGGGGCAACCCAATGCGGTGTATGGGTCTAGGATTGAGGCCCCGAAAGGGGTGACAGTGTCAGTGAAGCCGTCGAGGCTGGTGTTTAGTGGGGCGGTAAAGAAGCGGAGCTTTGTAGTGACGGTCGCGGTGGACAGGAGGAATGTGGTGTTTGGTGAGTCAGGTGCGGCGTTCGGGTCGCTTTACTGGAGCGACGGGAAGCACGTGGTTCGGAGCCCCATTGTAGTGACACAAATGGATCCCTTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I9 PA Peptidase_S8

Protein Analysis

772

Amino Acids

81.65

Weight (kDa)

9.36

Isoelectric Point (pI)

34.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I9	PF05922	35 - 105	1.4e-12	Peptidase inhibitor I9
Peptidase_S8	PF00082	128 - 597	2.5e-45	Subtilase family
PA	PF02225	377 - 464	1.8e-11	PA domain
fn3_6	PF17766	663 - 765	3.7e-28	Fibronectin type-III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004305)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G34980
fragaria_vesca	FvH4_2g01410 FvH4_3g19440
malus_domestica	MD05G1120100.v1.1 MD10G1123500.v1.1
prunus_persica	Prupe.8G164000_v2.0.a1
pyrus_communis	pycom05g11390 pycom10g10580
rosa_chinensis	RchiOBHm_Chr6g0244021 RchiOBHm_Chr6g0252351 RchiOBHm_Chr6g0252361
rosa_laevigata	RLG00000015390 RLG00000029339
rosa_multiflora	Rmu_co8423929.1_g000001 Rmu_sc0001251.1_g000002 Rmu_sc0006415.1_g000012 Rmu_sc0006711.1_g000006 Rmu_sc0007419.1_g000002
rosa_roxburghii	Rroxscaffold_6G00388820 Rroxscaffold_6G00388840
rosa_rugosa	Rorug05G0223500 Rorug05G0506700 Rorug05G0515900
rosa_samantha	Rh1CG388200 Rh2AG211900 Rh3DG088300 Rh5BG286100 Rh5DG294800 Rh6AG021200 Rh6CG013400 Rh6DG014700
rosa_wichuraiana	Rw6G001740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	762
AasI	GACNNNNNNGTC	3 cut(s)	395, 416, 1593
AatII	GACGTC	1 cut(s)	393
Acc65I	GGTACC	1 cut(s)	650
AccB1I	GGYRCC	1 cut(s)	650
AccBSI	CCGCTC	3 cut(s)	926, 1655, 1668
AccI	GTMKAC	1 cut(s)	201
AccII	CGCG	9 cut(s)	68, 111, 1001, 1025, 1201, 1236, 1676, 2012, 2195
AccIII	TCCGGA	2 cut(s)	1103, 2052
AcsI	RAATTY	6 cut(s)	50, 170, 503, 1042, 1311, 1834
AcuI	CTGAAG	2 cut(s)	1547, 1706
AcvI	CACGTG	2 cut(s)	1663, 2272
AcyI	GRCGYC	4 cut(s)	390, 417, 819, 1578
AdeI	CACNNNGTG	1 cut(s)	940
AfaI	GTAC	4 cut(s)	161, 652, 920, 1130
AflII	CTTAAG	1 cut(s)	92
AgeI	ACCGGT	1 cut(s)	1592
AgsI	TTSAA	3 cut(s)	49, 858, 1834
AjiI	CACGTC	1 cut(s)	199
AjnI	CCWGG	8 cut(s)	246, 291, 1144, 1203, 1350, 1546, 1855, 2021
AluBI	AGCT	4 cut(s)	532, 542, 746, 2178
AluI	AGCT	4 cut(s)	532, 542, 746, 2178
Alw21I	GWGCWC	1 cut(s)	544
Alw26I	GTCTC	5 cut(s)	256, 314, 485, 1072, 1334
Ama87I	CYCGRG	3 cut(s)	1248, 1489, 1944
Aor13HI	TCCGGA	2 cut(s)	1103, 2052
ApoI	RAATTY	6 cut(s)	50, 170, 503, 1042, 1311, 1834
ArsI	GACNNNNNNTTYG	4 cut(s)	968, 1000, 1251, 1283
AsiGI	ACCGGT	1 cut(s)	1592
Asp718I	GGTACC	1 cut(s)	650
AspLEI	GCGC	7 cut(s)	70, 1003, 1025, 1203, 1364, 1981, 2012
AsuC2I	CCSGG	6 cut(s)	1049, 1517, 1639, 1700, 1712, 1844
AsuHPI	GGTGA	8 cut(s)	588, 1000, 1394, 1688, 1867, 1978, 2131, 2234
AvaI	CYCGRG	3 cut(s)	1248, 1489, 1944
AvaII	GGWCC	7 cut(s)	295, 587, 971, 1195, 1572, 1586, 1948
BalI	TGGCCA	2 cut(s)	1256, 1355
BamHI	GGATCC	2 cut(s)	1852, 2305
BanI	GGYRCC	1 cut(s)	650
BanII	GRGCYC	5 cut(s)	345, 544, 1517, 1712, 2285
BauI	CACGAG	1 cut(s)	563
BbrPI	CACGTG	2 cut(s)	1663, 2272
BbsI	GAAGAC	1 cut(s)	942
Bbv12I	GWGCWC	1 cut(s)	544
BccI	CCATC	2 cut(s)	266, 1040
BcgI	CGANNNNNNTGC	4 cut(s)	887, 921, 2258, 2292
BciT130I	CCWGG	8 cut(s)	248, 293, 1146, 1205, 1352, 1548, 1857, 2023
BcnI	CCSGG	6 cut(s)	1049, 1517, 1639, 1700, 1712, 1844
BcoDI	GTCTC	5 cut(s)	256, 314, 485, 1072, 1334
BfaI	CTAG	1 cut(s)	2097
BfrI	CTTAAG	1 cut(s)	92
BglI	GCCNNNNNGGC	5 cut(s)	668, 1370, 1403, 1507, 2143
BlpI	GCTNAGC	1 cut(s)	1511
Bme18I	GGWCC	7 cut(s)	295, 587, 971, 1195, 1572, 1586, 1948
BmeT110I	CYCGRG	3 cut(s)	1248, 1489, 1944
BmgBI	CACGTC	1 cut(s)	199
BmsI	GCATC	1 cut(s)	625
BpiI	GAAGAC	1 cut(s)	942
BplI	GAGNNNNNCTC	2 cut(s)	1231, 1263
BpmI	CTGGAG	3 cut(s)	603, 1569, 2275
Bpu10I	CCTNAGC	1 cut(s)	344
Bpu1102I	GCTNAGC	1 cut(s)	1511
BpuMI	CCSGG	6 cut(s)	1049, 1517, 1639, 1700, 1712, 1844
Bsa29I	ATCGAT	1 cut(s)	1035
BsaAI	YACGTR	3 cut(s)	1402, 1663, 2272
BsaBI	GATNNNNATC	3 cut(s)	450, 1457, 2048
BsaHI	GRCGYC	4 cut(s)	390, 417, 819, 1578
BsaI	GGTCTC	2 cut(s)	256, 1334
BsaWI	WCCGGW	7 cut(s)	621, 1103, 1471, 1592, 1790, 1963, 2052
Bse118I	RCCGGY	6 cut(s)	413, 678, 959, 1280, 1465, 1592
Bse1I	ACTGG	2 cut(s)	161, 2258
Bse8I	GATNNNNATC	3 cut(s)	450, 1457, 2048
BseAI	TCCGGA	2 cut(s)	1103, 2052
BseBI	CCWGG	8 cut(s)	248, 293, 1146, 1205, 1352, 1548, 1857, 2023
BseCI	ATCGAT	1 cut(s)	1035
BseJI	GATNNNNATC	3 cut(s)	450, 1457, 2048
BseMII	CTCAG	3 cut(s)	69, 358, 1502
BseNI	ACTGG	2 cut(s)	161, 2258
BseRI	GAGGAG	4 cut(s)	8, 17, 26, 873
BseX3I	CGGCCG	4 cut(s)	676, 957, 1072, 1713
Bsh1236I	CGCG	9 cut(s)	68, 111, 1001, 1025, 1201, 1236, 1676, 2012, 2195
Bsh1285I	CGRYCG	8 cut(s)	626, 675, 679, 960, 1039, 1075, 1716, 2193
BshNI	GGYRCC	1 cut(s)	650
BshTI	ACCGGT	1 cut(s)	1592
BshVI	ATCGAT	1 cut(s)	1035
BsiEI	CGRYCG	8 cut(s)	626, 675, 679, 960, 1039, 1075, 1716, 2193
BsiHKAI	GWGCWC	1 cut(s)	544
BsiHKCI	CYCGRG	3 cut(s)	1248, 1489, 1944
BsiWI	CGTACG	1 cut(s)	918
BslFI	GGGAC	3 cut(s)	1459, 1599, 1654
BsmAI	GTCTC	5 cut(s)	256, 314, 485, 1072, 1334
BsmFI	GGGAC	3 cut(s)	1459, 1599, 1654
Bso31I	GGTCTC	2 cut(s)	256, 1334
BsoBI	CYCGRG	3 cut(s)	1248, 1489, 1944
Bsp1286I	GDGCHC	5 cut(s)	345, 544, 1517, 1712, 2285
Bsp13I	TCCGGA	2 cut(s)	1103, 2052
Bsp1720I	GCTNAGC	1 cut(s)	1511
Bsp19I	CCATGG	2 cut(s)	1422, 1647
BspCNI	CTCAG	3 cut(s)	68, 357, 1503
BspDI	ATCGAT	1 cut(s)	1035
BspEI	TCCGGA	2 cut(s)	1103, 2052
BspFNI	CGCG	9 cut(s)	68, 111, 1001, 1025, 1201, 1236, 1676, 2012, 2195
BspT107I	GGYRCC	1 cut(s)	650
BspTI	CTTAAG	1 cut(s)	92
BspTNI	GGTCTC	2 cut(s)	256, 1334
BsrBI	CCGCTC	3 cut(s)	926, 1655, 1668
BsrFI	RCCGGY	6 cut(s)	413, 678, 959, 1280, 1465, 1592
BsrI	ACTGG	2 cut(s)	161, 2258
BssAI	RCCGGY	6 cut(s)	413, 678, 959, 1280, 1465, 1592
BssNI	GRCGYC	4 cut(s)	390, 417, 819, 1578
BssSI	CACGAG	1 cut(s)	563
BssT1I	CCWWGG	4 cut(s)	1004, 1422, 1441, 1647
Bst2BI	CACGAG	1 cut(s)	563
Bst2UI	CCWGG	8 cut(s)	248, 293, 1146, 1205, 1352, 1548, 1857, 2023
Bst6I	CTCTTC	1 cut(s)	506
BstACI	GRCGYC	4 cut(s)	390, 417, 819, 1578
BstAFI	CTTAAG	1 cut(s)	92
BstBAI	YACGTR	3 cut(s)	1402, 1663, 2272
BstDEI	CTNAG	5 cut(s)	55, 344, 1082, 1264, 1511
BstDSI	CCRYGG	7 cut(s)	217, 666, 1234, 1422, 1581, 1647, 1674
BstENI	CCTNNNNNAGG	1 cut(s)	1116
BstFNI	CGCG	9 cut(s)	68, 111, 1001, 1025, 1201, 1236, 1676, 2012, 2195
BstHHI	GCGC	7 cut(s)	70, 1003, 1025, 1203, 1364, 1981, 2012
BstMAI	GTCTC	5 cut(s)	256, 314, 485, 1072, 1334
BstMCI	CGRYCG	8 cut(s)	626, 675, 679, 960, 1039, 1075, 1716, 2193
BstNI	CCWGG	8 cut(s)	248, 293, 1146, 1205, 1352, 1548, 1857, 2023
BstNSI	RCATGY	3 cut(s)	703, 985, 1162
BstUI	CGCG	9 cut(s)	68, 111, 1001, 1025, 1201, 1236, 1676, 2012, 2195
BstV2I	GAAGAC	1 cut(s)	942
BstX2I	RGATCY	4 cut(s)	189, 1724, 1852, 2305
BstXI	CCANNNNNNTGG	1 cut(s)	1597
BstYI	RGATCY	4 cut(s)	189, 1724, 1852, 2305
BstZI	CGGCCG	4 cut(s)	676, 957, 1072, 1713
Bsu15I	ATCGAT	1 cut(s)	1035
BsuTUI	ATCGAT	1 cut(s)	1035
BtgI	CCRYGG	7 cut(s)	217, 666, 1234, 1422, 1581, 1647, 1674
BtrI	CACGTC	1 cut(s)	199
BtsIMutI	CAGTG	4 cut(s)	154, 1602, 2130, 2136
CfoI	GCGC	7 cut(s)	70, 1003, 1025, 1203, 1364, 1981, 2012
Cfr10I	RCCGGY	6 cut(s)	413, 678, 959, 1280, 1465, 1592
Cfr42I	CCGCGG	2 cut(s)	1237, 1677
ClaI	ATCGAT	1 cut(s)	1035
CseI	GACGC	7 cut(s)	406, 419, 827, 1349, 1394, 1438, 1586
CsiI	ACCWGGT	1 cut(s)	246
Csp6I	GTAC	4 cut(s)	160, 651, 919, 1129
CspAI	ACCGGT	1 cut(s)	1592
CspCI	CAANNNNNGTGG	2 cut(s)	318, 353
CviAII	CATG	9 cut(s)	647, 700, 982, 1159, 1180, 1423, 1648, 1732, 1774
CviQI	GTAC	4 cut(s)	160, 651, 919, 1129
DdeI	CTNAG	5 cut(s)	55, 344, 1082, 1264, 1511
DraIII	CACNNNGTG	1 cut(s)	940
DrdI	GACNNNNNNGTC	3 cut(s)	395, 416, 1593
DseDI	GACNNNNNNGTC	3 cut(s)	395, 416, 1593
EagI	CGGCCG	4 cut(s)	676, 957, 1072, 1713
Eam1104I	CTCTTC	1 cut(s)	506
EarI	CTCTTC	1 cut(s)	506
EciI	GGCGGA	3 cut(s)	217, 244, 1717
Ecl136II	GAGCTC	1 cut(s)	542
EclXI	CGGCCG	4 cut(s)	676, 957, 1072, 1713
Eco130I	CCWWGG	4 cut(s)	1004, 1422, 1441, 1647
Eco147I	AGGCCT	1 cut(s)	1397
Eco24I	GRGCYC	5 cut(s)	345, 544, 1517, 1712, 2285
Eco31I	GGTCTC	2 cut(s)	256, 1334
Eco32I	GATATC	1 cut(s)	594
Eco47I	GGWCC	7 cut(s)	295, 587, 971, 1195, 1572, 1586, 1948
Eco52I	CGGCCG	4 cut(s)	676, 957, 1072, 1713
Eco53kI	GAGCTC	1 cut(s)	542
Eco57I	CTGAAG	2 cut(s)	1547, 1706
Eco72I	CACGTG	2 cut(s)	1663, 2272
Eco88I	CYCGRG	3 cut(s)	1248, 1489, 1944
EcoICRI	GAGCTC	1 cut(s)	542
EcoNI	CCTNNNNNAGG	1 cut(s)	1116
EcoO109I	RGGNCCY	2 cut(s)	1586, 2107
EcoRI	GAATTC	1 cut(s)	170
EcoRII	CCWGG	8 cut(s)	246, 291, 1144, 1203, 1350, 1546, 1855, 2021
EcoRV	GATATC	1 cut(s)	594
EcoT14I	CCWWGG	4 cut(s)	1004, 1422, 1441, 1647
EcoT38I	GRGCYC	5 cut(s)	345, 544, 1517, 1712, 2285
ErhI	CCWWGG	4 cut(s)	1004, 1422, 1441, 1647
FaeI	CATG	9 cut(s)	650, 703, 985, 1162, 1183, 1426, 1651, 1735, 1777
FalI	AAGNNNNNCTT	2 cut(s)	2162, 2194
FaqI	GGGAC	3 cut(s)	1459, 1599, 1654
FatI	CATG	9 cut(s)	646, 699, 981, 1158, 1179, 1422, 1647, 1731, 1773
FauI	CCCGC	6 cut(s)	189, 1018, 1206, 1229, 1816, 2020
FblI	GTMKAC	1 cut(s)	201
FriOI	GRGCYC	5 cut(s)	345, 544, 1517, 1712, 2285
FspBI	CTAG	1 cut(s)	2097
GlaI	GCGC	7 cut(s)	69, 1002, 1024, 1202, 1363, 1980, 2011
GsuI	CTGGAG	3 cut(s)	603, 1569, 2275
HgaI	GACGC	7 cut(s)	406, 419, 827, 1349, 1394, 1438, 1586
HhaI	GCGC	7 cut(s)	70, 1003, 1025, 1203, 1364, 1981, 2012
Hin1I	GRCGYC	4 cut(s)	390, 417, 819, 1578
Hin1II	CATG	9 cut(s)	650, 703, 985, 1162, 1183, 1426, 1651, 1735, 1777
Hin6I	GCGC	7 cut(s)	68, 1001, 1023, 1201, 1362, 1979, 2010
HinP1I	GCGC	7 cut(s)	68, 1001, 1023, 1201, 1362, 1979, 2010
HincII	GTYRAC	2 cut(s)	115, 1555
HindII	GTYRAC	2 cut(s)	115, 1555
HphI	GGTGA	8 cut(s)	588, 1000, 1394, 1688, 1867, 1978, 2131, 2234
Hpy166II	GTNNAC	4 cut(s)	115, 202, 1555, 2200
Hpy188III	TCNNGA	8 cut(s)	242, 346, 896, 1104, 1193, 1270, 1598, 2053
Hpy8I	GTNNAC	4 cut(s)	115, 202, 1555, 2200
HpyAV	CCTTC	4 cut(s)	109, 282, 472, 769
HpyCH4IV	ACGT	6 cut(s)	198, 390, 1401, 1662, 1890, 2271
HpyCH4V	TGCA	2 cut(s)	522, 1179
HpyF3I	CTNAG	5 cut(s)	55, 344, 1082, 1264, 1511
HpySE526I	ACGT	6 cut(s)	198, 390, 1401, 1662, 1890, 2271
Hsp92I	GRCGYC	4 cut(s)	390, 417, 819, 1578
Hsp92II	CATG	9 cut(s)	650, 703, 985, 1162, 1183, 1426, 1651, 1735, 1777
HspAI	GCGC	7 cut(s)	68, 1001, 1023, 1201, 1362, 1979, 2010
KflI	GGGWCCC	1 cut(s)	1586
Kpn2I	TCCGGA	2 cut(s)	1103, 2052
KpnI	GGTACC	1 cut(s)	654
KroI	GCCGGC	3 cut(s)	678, 959, 1280
KroNI	GCCGGC	3 cut(s)	680, 961, 1282
KspI	CCGCGG	2 cut(s)	1237, 1677
LweI	GCATC	1 cut(s)	625
MabI	ACCWGGT	1 cut(s)	246
MaeI	CTAG	1 cut(s)	2097
MaeII	ACGT	6 cut(s)	198, 390, 1401, 1662, 1890, 2271
MbiI	CCGCTC	3 cut(s)	926, 1655, 1668
MboII	GAAGA	5 cut(s)	523, 541, 942, 1285, 1981
MflI	RGATCY	4 cut(s)	189, 1724, 1852, 2305
MhlI	GDGCHC	5 cut(s)	345, 544, 1517, 1712, 2285
MlsI	TGGCCA	2 cut(s)	1256, 1355
MluCI	AATT	9 cut(s)	50, 170, 332, 503, 1042, 1218, 1311, 1834, 1993
MluNI	TGGCCA	2 cut(s)	1256, 1355
MlyI	GAGTC	5 cut(s)	316, 741, 788, 1560, 2234
MmeI	TCCRAC	6 cut(s)	288, 348, 379, 411, 1270, 1975
Mox20I	TGGCCA	2 cut(s)	1256, 1355
MroI	TCCGGA	2 cut(s)	1103, 2052
MroNI	GCCGGC	3 cut(s)	678, 959, 1280
MscI	TGGCCA	2 cut(s)	1256, 1355
MseI	TTAA	3 cut(s)	93, 828, 1626
MslI	CAYNNNNRTG	2 cut(s)	986, 2291
Msp20I	TGGCCA	2 cut(s)	1256, 1355
MspA1I	CMGCKG	2 cut(s)	1236, 1676
MspCI	CTTAAG	1 cut(s)	92
MvaI	CCWGG	8 cut(s)	248, 293, 1146, 1205, 1352, 1548, 1857, 2023
MvnI	CGCG	9 cut(s)	68, 111, 1001, 1025, 1201, 1236, 1676, 2012, 2195
NaeI	GCCGGC	3 cut(s)	680, 961, 1282
NciI	CCSGG	6 cut(s)	1049, 1517, 1639, 1700, 1712, 1844
NcoI	CCATGG	2 cut(s)	1422, 1647
NgoMIV	GCCGGC	3 cut(s)	678, 959, 1280
NlaIII	CATG	9 cut(s)	650, 703, 985, 1162, 1183, 1426, 1651, 1735, 1777
NmeAIII	GCCGAG	2 cut(s)	654, 935
NmuCI	GTSAC	8 cut(s)	988, 1229, 1382, 1750, 2059, 2119, 2185, 2293
NspI	RCATGY	3 cut(s)	703, 985, 1162
PaeI	GCATGC	1 cut(s)	1162
PaeR7I	CTCGAG	1 cut(s)	1248
PceI	AGGCCT	1 cut(s)	1397
PcsI	WCGNNNNNNNCGW	4 cut(s)	867, 942, 1374, 2268
PdiI	GCCGGC	3 cut(s)	680, 961, 1282
PfeI	GAWTC	9 cut(s)	371, 451, 463, 546, 779, 1458, 1522, 1930, 1987
Pfl23II	CGTACG	1 cut(s)	918
PflFI	GACNNNGTC	2 cut(s)	248, 2125
PfoI	TCCNGGA	2 cut(s)	291, 1637
PinAI	ACCGGT	1 cut(s)	1592
Ple19I	CGATCG	2 cut(s)	675, 1039
PleI	GAGTC	5 cut(s)	315, 740, 788, 1559, 2233
PmaCI	CACGTG	2 cut(s)	1663, 2272
PmlI	CACGTG	2 cut(s)	1663, 2272
PpsI	GAGTC	5 cut(s)	315, 740, 788, 1559, 2233
Ppu21I	YACGTR	3 cut(s)	1402, 1663, 2272
PpuMI	RGGWCCY	1 cut(s)	1586
PsiI	TTATAA	1 cut(s)	762
Psp124BI	GAGCTC	1 cut(s)	544
Psp5II	RGGWCCY	1 cut(s)	1586
Psp6I	CCWGG	8 cut(s)	246, 291, 1144, 1203, 1350, 1546, 1855, 2021
PspCI	CACGTG	2 cut(s)	1663, 2272
PspGI	CCWGG	8 cut(s)	246, 291, 1144, 1203, 1350, 1546, 1855, 2021
PspLI	CGTACG	1 cut(s)	918
PspPPI	RGGWCCY	1 cut(s)	1586
PspXI	VCTCGAGB	1 cut(s)	1248
PsuI	RGATCY	4 cut(s)	189, 1724, 1852, 2305
PsyI	GACNNNGTC	2 cut(s)	248, 2125
PvuI	CGATCG	2 cut(s)	675, 1039
RsaI	GTAC	4 cut(s)	161, 652, 920, 1130
RsaNI	GTAC	4 cut(s)	160, 651, 919, 1129
RseI	CAYNNNNRTG	2 cut(s)	986, 2291
SacI	GAGCTC	1 cut(s)	544
SacII	CCGCGG	2 cut(s)	1237, 1677
SaqAI	TTAA	3 cut(s)	93, 828, 1626
SchI	GAGTC	5 cut(s)	316, 741, 788, 1560, 2234
SduI	GDGCHC	5 cut(s)	345, 544, 1517, 1712, 2285
SexAI	ACCWGGT	1 cut(s)	246
SfaNI	GCATC	1 cut(s)	625
SfiI	GGCCNNNNNGGCC	1 cut(s)	1403
Sfr274I	CTCGAG	1 cut(s)	1248
Sfr303I	CCGCGG	2 cut(s)	1237, 1677
SgrAI	CRCCGGYG	1 cut(s)	413
SgrBI	CCGCGG	2 cut(s)	1237, 1677
SinI	GGWCC	7 cut(s)	295, 587, 971, 1195, 1572, 1586, 1948
SlaI	CTCGAG	1 cut(s)	1248
SmiMI	CAYNNNNRTG	2 cut(s)	986, 2291
SmlI	CTYRAG	2 cut(s)	92, 1248
SmoI	CTYRAG	2 cut(s)	92, 1248
SphI	GCATGC	1 cut(s)	1162
Sse9I	AATT	9 cut(s)	50, 170, 332, 503, 1042, 1218, 1311, 1834, 1993
SseBI	AGGCCT	1 cut(s)	1397
SspMI	CTAG	1 cut(s)	2097
SstI	GAGCTC	1 cut(s)	544
StuI	AGGCCT	1 cut(s)	1397
StyI	CCWWGG	4 cut(s)	1004, 1422, 1441, 1647
TaiI	ACGT	6 cut(s)	201, 393, 1404, 1665, 1893, 2274
TaqII	GACCGA	2 cut(s)	1589, 1793
TasI	AATT	9 cut(s)	50, 170, 332, 503, 1042, 1218, 1311, 1834, 1993
TfiI	GAWTC	9 cut(s)	371, 451, 463, 546, 779, 1458, 1522, 1930, 1987
Tru1I	TTAA	3 cut(s)	93, 828, 1626
Tru9I	TTAA	3 cut(s)	93, 828, 1626
TscAI	CASTG	4 cut(s)	161, 1609, 2130, 2136
TseFI	GTSAC	8 cut(s)	988, 1229, 1382, 1750, 2059, 2119, 2185, 2293
Tsp45I	GTSAC	8 cut(s)	988, 1229, 1382, 1750, 2059, 2119, 2185, 2293
TspDTI	ATGAA	2 cut(s)	91, 541
TspGWI	ACGGA	5 cut(s)	458, 876, 936, 951, 1323
TspRI	CASTG	4 cut(s)	161, 1609, 2130, 2136
Tth111I	GACNNNGTC	2 cut(s)	248, 2125
Vha464I	CTTAAG	1 cut(s)	92
VpaK11BI	GGWCC	7 cut(s)	295, 587, 971, 1195, 1572, 1586, 1948
XagI	CCTNNNNNAGG	1 cut(s)	1116
XapI	RAATTY	6 cut(s)	50, 170, 503, 1042, 1311, 1834
XceI	RCATGY	3 cut(s)	703, 985, 1162
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1420
XhoI	CTCGAG	1 cut(s)	1248
XmiI	GTMKAC	1 cut(s)	201
XspI	CTAG	1 cut(s)	2097
ZraI	GACGTC	1 cut(s)	391

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.