RLG00000029339

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0004305
Physical Location & Seq
Reverse (-)
36725508 .. 36725849
342 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000029339

Sequence Viewer

Length: 342 bp
ATGTCTGCATCCCATTACCACTGGTACACCTTGGAGTTCACCGACCCACCTCAAATCCTCCACATATACGACACCGTTTTCCATGGCTTCTCTGCAACTCTCACCTCCGACCAAGTTGCCTCCCTTAGCCAACACCCTGTCATTCTCCACGTCTTCGAAGACCGCCGCCGCCAGCTCCACACCACCCGCTCGCCACAGTTCCTAGGCCTGAGAAACCAGAAGGGGTTGTGGTTGGAATCGGACTACTGCTCCGACATCATCGTTGGGGTTTTCGATACTGGAGTTTGGCCGGAGCGTCGGAGCTTTTCCAATAGGAATCTCAATATGGTTCTGAAGTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

114

Amino Acids

13.29

Weight (kDa)

6.64

Isoelectric Point (pI)

66.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 14 - 60 3.1e-12 Peptidase inhibitor I9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 189
AciI CCGC 4 cut(s) 163, 166, 169, 187
AcoI YGGCCR 1 cut(s) 287
AfaI GTAC 1 cut(s) 26
AjiI CACGTC 1 cut(s) 151
AluBI AGCT 2 cut(s) 175, 303
AluI AGCT 2 cut(s) 175, 303
AoxI GGCC 2 cut(s) 205, 287
AspA2I CCTAGG 1 cut(s) 202
AsuHPI GGTGA 2 cut(s) 31, 94
AsuII TTCGAA 1 cut(s) 156
AvrII CCTAGG 1 cut(s) 202
BaeI ACNNNNGTAYC 2 cut(s) 267, 300
BbsI GAAGAC 2 cut(s) 145, 165
BcgI CGANNNNNNTGC 2 cut(s) 98, 132
BfaI CTAG 1 cut(s) 203
BisI GCNGC 2 cut(s) 166, 169
BlnI CCTAGG 1 cut(s) 202
BlsI GCNGC 2 cut(s) 167, 170
BmgBI CACGTC 1 cut(s) 151
BmsI GCATC 1 cut(s) 17
BpiI GAAGAC 2 cut(s) 145, 165
BpmI CTGGAG 1 cut(s) 300
Bpu10I CCTNAGC 1 cut(s) 125
Bpu14I TTCGAA 1 cut(s) 156
BsaJI CCNNGG 3 cut(s) 30, 82, 202
BsaXI ACNNNNNCTCC 2 cut(s) 233, 263
Bse1I ACTGG 2 cut(s) 26, 283
BseDI CCNNGG 3 cut(s) 30, 82, 202
BseGI GGATG 1 cut(s) 8
BseMII CTCAG 1 cut(s) 200
BseNI ACTGG 2 cut(s) 26, 283
BshFI GGCC 2 cut(s) 207, 289
BsiSI CCGG 1 cut(s) 290
BsnI GGCC 2 cut(s) 207, 289
Bsp119I TTCGAA 1 cut(s) 156
Bsp19I CCATGG 1 cut(s) 82
BspACI CCGC 4 cut(s) 163, 166, 169, 187
BspANI GGCC 2 cut(s) 207, 289
BspCNI CTCAG 1 cut(s) 201
BspT104I TTCGAA 1 cut(s) 156
BsrBI CCGCTC 1 cut(s) 189
BsrI ACTGG 2 cut(s) 26, 283
BssECI CCNNGG 3 cut(s) 30, 82, 202
BssT1I CCWWGG 3 cut(s) 30, 82, 202
Bst4CI ACNGT 2 cut(s) 76, 198
BstBI TTCGAA 1 cut(s) 156
BstC8I GCNNGC 2 cut(s) 173, 191
BstDEI CTNAG 2 cut(s) 125, 209
BstDSI CCRYGG 1 cut(s) 82
BstF5I GGATG 1 cut(s) 8
BstV2I GAAGAC 2 cut(s) 145, 165
BsuRI GGCC 2 cut(s) 207, 289
BtgI CCRYGG 1 cut(s) 82
BtrI CACGTC 1 cut(s) 151
BtsCI GGATG 1 cut(s) 8
BtsIMutI CAGTG 1 cut(s) 19
Cac8I GCNNGC 2 cut(s) 173, 191
CseI GACGC 1 cut(s) 284
Csp6I GTAC 1 cut(s) 25
CviAII CATG 1 cut(s) 83
CviJI RGCY 6 cut(s) 87, 129, 175, 207, 289, 303
CviKI_1 RGCY 6 cut(s) 87, 129, 175, 207, 289, 303
CviQI GTAC 1 cut(s) 25
DdeI CTNAG 2 cut(s) 125, 209
EaeI YGGCCR 1 cut(s) 287
Eco130I CCWWGG 3 cut(s) 30, 82, 202
Eco147I AGGCCT 1 cut(s) 207
EcoT14I CCWWGG 3 cut(s) 30, 82, 202
ErhI CCWWGG 3 cut(s) 30, 82, 202
FaeI CATG 1 cut(s) 86
FaiI YATR 4 cut(s) 65, 67, 84, 326
FatI CATG 1 cut(s) 82
FauI CCCGC 1 cut(s) 194
Fnu4HI GCNGC 2 cut(s) 166, 169
Fsp4HI GCNGC 2 cut(s) 166, 169
FspBI CTAG 1 cut(s) 203
GluI GCNGC 2 cut(s) 166, 169
GsuI CTGGAG 1 cut(s) 300
HaeIII GGCC 2 cut(s) 207, 289
HapII CCGG 1 cut(s) 290
HgaI GACGC 1 cut(s) 284
Hin1II CATG 1 cut(s) 86
HinfI GANTC 2 cut(s) 236, 316
HpaII CCGG 1 cut(s) 290
HphI GGTGA 2 cut(s) 31, 94
Hpy166II GTNNAC 2 cut(s) 27, 39
Hpy188I TCNGA 5 cut(s) 109, 241, 253, 300, 333
Hpy8I GTNNAC 2 cut(s) 27, 39
Hpy99I CGWCG 1 cut(s) 300
HpyAV CCTTC 1 cut(s) 214
HpyCH4III ACNGT 2 cut(s) 76, 198
HpyCH4IV ACGT 1 cut(s) 150
HpyCH4V TGCA 2 cut(s) 8, 95
HpyF3I CTNAG 2 cut(s) 125, 209
HpySE526I ACGT 1 cut(s) 150
Hsp92II CATG 1 cut(s) 86
LmnI GCTCC 4 cut(s) 180, 254, 292, 300
LpnPI CCDG 7 cut(s) 7, 150, 185, 221, 230, 264, 303
LweI GCATC 1 cut(s) 17
MaeI CTAG 1 cut(s) 203
MaeII ACGT 1 cut(s) 150
MbiI CCGCTC 1 cut(s) 189
MboII GAAGA 2 cut(s) 145, 170
MmeI TCCRAC 4 cut(s) 132, 213, 276, 278
MnlI CCTC 4 cut(s) 60, 68, 115, 130
MspI CCGG 1 cut(s) 290
NcoI CCATGG 1 cut(s) 82
NlaIII CATG 1 cut(s) 86
NspV TTCGAA 1 cut(s) 156
PceI AGGCCT 1 cut(s) 207
PfeI GAWTC 2 cut(s) 236, 316
PkrI GCNGC 2 cut(s) 167, 170
RsaI GTAC 1 cut(s) 26
RsaNI GTAC 1 cut(s) 25
SatI GCNGC 2 cut(s) 166, 169
SetI ASST 6 cut(s) 32, 52, 107, 153, 177, 305
SfaNI GCATC 1 cut(s) 17
SfuI TTCGAA 1 cut(s) 156
SseBI AGGCCT 1 cut(s) 207
SsiI CCGC 4 cut(s) 163, 166, 169, 187
SspMI CTAG 1 cut(s) 203
StuI AGGCCT 1 cut(s) 207
StyI CCWWGG 3 cut(s) 30, 82, 202
TaaI ACNGT 2 cut(s) 76, 198
TaiI ACGT 1 cut(s) 153
TaqI TCGA 2 cut(s) 156, 273
TauI GCSGC 2 cut(s) 168, 171
TfiI GAWTC 2 cut(s) 236, 316
TscAI CASTG 1 cut(s) 26
TspRI CASTG 1 cut(s) 26
XmaJI CCTAGG 1 cut(s) 202
XspI CTAG 1 cut(s) 203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.