Rh1CG388200

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0004305
Physical Location & Seq
Forward (+)
65748976 .. 65749313
338 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG388200.1

Sequence Viewer

Length: 338 bp
ATGTCTGCGTCCCATTACCACTGGTACACATCGGAGTTCGCCGACCCACCTCAAATCCTCCACGTATACGACACCGTTTTCCATGGCTTCTCTGCAACTCTCACCTCCGACCAAGTTGCCTCCCTCCGCCAACACCTTGCCGTTCTCCACGTCTTCGAAGACCGCCGCCGCCAGCTCCACACCACCCACTCGCTGCAGTTCCTGGGCCTGAGAAACCAGAAGGGGCTGTGGTCGGAATCGGACTACAGCTCCGACGTCATCGTCGGGGTTTTCGATACTGGAGTTTGGCCGGAGCGTCAGAGCTTTTCCTATAGGAATCTCGGTCCGGTTCCGAAGCG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

112

Amino Acids

12.93

Weight (kDa)

6.42

Isoelectric Point (pI)

56.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 4 - 60 2.3e-12 Peptidase inhibitor I9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 260
AatII GACGTC 1 cut(s) 258
AccI GTMKAC 1 cut(s) 66
AciI CCGC 4 cut(s) 127, 163, 166, 169
AcoI YGGCCR 1 cut(s) 287
AcyI GRCGYC 1 cut(s) 255
AfaI GTAC 1 cut(s) 26
AjiI CACGTC 1 cut(s) 151
AjnI CCWGG 1 cut(s) 201
AluBI AGCT 3 cut(s) 175, 249, 303
AluI AGCT 3 cut(s) 175, 249, 303
AlwNI CAGNNNCTG 1 cut(s) 202
AoxI GGCC 2 cut(s) 205, 287
ApeKI GCWGC 1 cut(s) 193
AspS9I GGNCC 2 cut(s) 205, 323
AsuHPI GGTGA 1 cut(s) 94
AsuII TTCGAA 1 cut(s) 156
AvaII GGWCC 1 cut(s) 323
BaeI ACNNNNGTAYC 2 cut(s) 267, 300
BbsI GAAGAC 2 cut(s) 145, 165
BbvI GCAGC 1 cut(s) 180
BceAI ACGGC 1 cut(s) 125
BcgI CGANNNNNNTGC 2 cut(s) 98, 132
BciT130I CCWGG 1 cut(s) 203
BfmI CTRYAG 3 cut(s) 194, 244, 310
BisI GCNGC 3 cut(s) 166, 169, 194
BlsI GCNGC 3 cut(s) 167, 170, 195
Bme1390I CCNGG 1 cut(s) 203
Bme18I GGWCC 1 cut(s) 323
BmgBI CACGTC 1 cut(s) 151
BmgT120I GGNCC 2 cut(s) 205, 323
BmiI GGNNCC 1 cut(s) 330
BmrFI CCNGG 1 cut(s) 203
BpiI GAAGAC 2 cut(s) 145, 165
BpmI CTGGAG 1 cut(s) 300
Bpu14I TTCGAA 1 cut(s) 156
BsaAI YACGTR 1 cut(s) 64
BsaHI GRCGYC 1 cut(s) 255
BsaJI CCNNGG 2 cut(s) 82, 202
BsaWI WCCGGW 1 cut(s) 325
BsaXI ACNNNNNCTCC 2 cut(s) 233, 263
Bse1I ACTGG 2 cut(s) 26, 283
BseBI CCWGG 1 cut(s) 203
BseDI CCNNGG 2 cut(s) 82, 202
BseMII CTCAG 1 cut(s) 200
BseNI ACTGG 2 cut(s) 26, 283
BseXI GCAGC 1 cut(s) 180
BshFI GGCC 2 cut(s) 207, 289
BsiSI CCGG 2 cut(s) 290, 326
BsnI GGCC 2 cut(s) 207, 289
Bsp119I TTCGAA 1 cut(s) 156
Bsp19I CCATGG 1 cut(s) 82
BspACI CCGC 4 cut(s) 127, 163, 166, 169
BspANI GGCC 2 cut(s) 207, 289
BspCNI CTCAG 1 cut(s) 201
BspLI GGNNCC 1 cut(s) 330
BspMAI CTGCAG 1 cut(s) 198
BspT104I TTCGAA 1 cut(s) 156
BsrI ACTGG 2 cut(s) 26, 283
BssECI CCNNGG 2 cut(s) 82, 202
BssNAI GTATAC 1 cut(s) 67
BssNI GRCGYC 1 cut(s) 255
BssT1I CCWWGG 1 cut(s) 82
Bst1107I GTATAC 1 cut(s) 67
Bst2UI CCWGG 1 cut(s) 203
Bst4CI ACNGT 1 cut(s) 76
BstACI GRCGYC 1 cut(s) 255
BstBAI YACGTR 1 cut(s) 64
BstBI TTCGAA 1 cut(s) 156
BstC8I GCNNGC 1 cut(s) 173
BstDEI CTNAG 1 cut(s) 209
BstDSI CCRYGG 1 cut(s) 82
BstNI CCWGG 1 cut(s) 203
BstSCI CCNGG 1 cut(s) 201
BstSFI CTRYAG 3 cut(s) 194, 244, 310
BstV1I GCAGC 1 cut(s) 180
BstV2I GAAGAC 2 cut(s) 145, 165
BstZ17I GTATAC 1 cut(s) 67
BsuRI GGCC 2 cut(s) 207, 289
BtgI CCRYGG 1 cut(s) 82
BtrI CACGTC 1 cut(s) 151
BtsIMutI CAGTG 1 cut(s) 19
Cac8I GCNNGC 1 cut(s) 173
CaiI CAGNNNCTG 1 cut(s) 202
Cfr13I GGNCC 2 cut(s) 205, 323
CpoI CGGWCCG 1 cut(s) 323
CseI GACGC 1 cut(s) 284
Csp6I GTAC 1 cut(s) 25
CspI CGGWCCG 1 cut(s) 323
CviAII CATG 1 cut(s) 83
CviJI RGCY 7 cut(s) 87, 175, 207, 226, 249, 289, 303
CviKI_1 RGCY 7 cut(s) 87, 175, 207, 226, 249, 289, 303
CviQI GTAC 1 cut(s) 25
DdeI CTNAG 1 cut(s) 209
DrdI GACNNNNNNGTC 1 cut(s) 260
DseDI GACNNNNNNGTC 1 cut(s) 260
EaeI YGGCCR 1 cut(s) 287
EciI GGCGGA 1 cut(s) 116
Eco130I CCWWGG 1 cut(s) 82
Eco47I GGWCC 1 cut(s) 323
EcoRII CCWGG 1 cut(s) 201
EcoT14I CCWWGG 1 cut(s) 82
ErhI CCWWGG 1 cut(s) 82
FaeI CATG 1 cut(s) 86
FaiI YATR 3 cut(s) 67, 84, 312
FatI CATG 1 cut(s) 82
FblI GTMKAC 1 cut(s) 66
Fnu4HI GCNGC 3 cut(s) 166, 169, 194
Fsp4HI GCNGC 3 cut(s) 166, 169, 194
GluI GCNGC 3 cut(s) 166, 169, 194
GsuI CTGGAG 1 cut(s) 300
HaeIII GGCC 2 cut(s) 207, 289
HapII CCGG 2 cut(s) 290, 326
HgaI GACGC 1 cut(s) 284
Hin1I GRCGYC 1 cut(s) 255
Hin1II CATG 1 cut(s) 86
HinfI GANTC 2 cut(s) 236, 316
HpaII CCGG 2 cut(s) 290, 326
HphI GGTGA 1 cut(s) 94
Hpy166II GTNNAC 2 cut(s) 27, 67
Hpy188I TCNGA 7 cut(s) 34, 109, 235, 241, 253, 300, 333
Hpy8I GTNNAC 2 cut(s) 27, 67
Hpy99I CGWCG 2 cut(s) 257, 266
HpyAV CCTTC 1 cut(s) 214
HpyCH4III ACNGT 1 cut(s) 76
HpyCH4IV ACGT 3 cut(s) 63, 150, 255
HpyCH4V TGCA 2 cut(s) 95, 196
HpyF3I CTNAG 1 cut(s) 209
HpySE526I ACGT 3 cut(s) 63, 150, 255
Hsp92I GRCGYC 1 cut(s) 255
Hsp92II CATG 1 cut(s) 86
LmnI GCTCC 3 cut(s) 180, 254, 292
LpnPI CCDG 8 cut(s) 7, 185, 188, 215, 221, 230, 264, 303
Lsp1109I GCAGC 1 cut(s) 180
MaeII ACGT 3 cut(s) 63, 150, 255
MboII GAAGA 2 cut(s) 145, 170
MmeI TCCRAC 3 cut(s) 132, 213, 276
MnlI CCTC 5 cut(s) 60, 68, 115, 130, 134
MspI CCGG 2 cut(s) 290, 326
MspR9I CCNGG 1 cut(s) 203
MvaI CCWGG 1 cut(s) 203
NcoI CCATGG 1 cut(s) 82
NlaIII CATG 1 cut(s) 86
NlaIV GGNNCC 1 cut(s) 330
NspV TTCGAA 1 cut(s) 156
PcsI WCGNNNNNNNCGW 1 cut(s) 270
PfeI GAWTC 2 cut(s) 236, 316
PkrI GCNGC 3 cut(s) 167, 170, 195
Ppu21I YACGTR 1 cut(s) 64
Psp6I CCWGG 1 cut(s) 201
PspGI CCWGG 1 cut(s) 201
PspN4I GGNNCC 1 cut(s) 330
PspPI GGNCC 2 cut(s) 205, 323
PstI CTGCAG 1 cut(s) 198
PstNI CAGNNNCTG 1 cut(s) 202
RsaI GTAC 1 cut(s) 26
RsaNI GTAC 1 cut(s) 25
Rsr2I CGGWCCG 1 cut(s) 323
RsrII CGGWCCG 1 cut(s) 323
SatI GCNGC 3 cut(s) 166, 169, 194
Sau96I GGNCC 2 cut(s) 205, 323
ScrFI CCNGG 1 cut(s) 203
SetI ASST 9 cut(s) 52, 66, 107, 138, 153, 177, 251, 258, 305
SfcI CTRYAG 3 cut(s) 194, 244, 310
SfuI TTCGAA 1 cut(s) 156
SinI GGWCC 1 cut(s) 323
SsiI CCGC 4 cut(s) 127, 163, 166, 169
StyD4I CCNGG 1 cut(s) 201
StyI CCWWGG 1 cut(s) 82
TaaI ACNGT 1 cut(s) 76
TaiI ACGT 3 cut(s) 66, 153, 258
TaqI TCGA 2 cut(s) 156, 273
TaqII GACCGA 1 cut(s) 311
TauI GCSGC 2 cut(s) 168, 171
TfiI GAWTC 2 cut(s) 236, 316
TscAI CASTG 1 cut(s) 26
TseI GCWGC 1 cut(s) 193
TspRI CASTG 1 cut(s) 26
VpaK11BI GGWCC 1 cut(s) 323
XmiI GTMKAC 1 cut(s) 66
ZraI GACGTC 1 cut(s) 256
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.