Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0004305

Physical Location & Seq

Genomic Coordinates Forward (+)

24163537 .. 24165894

Gene Structure 2358 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1120100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2358 bp

ATGCAGTCCTTCCACTTCACTCTCACCCTCACTCTATTTCCAATGGCTTCCACTCCTCTCCTCCCGCTCCTCTTCCTCCTCACATTCTTCAAATTCTCAGCTCAAATCGCGCCGCAAACCGCCGATCAAACCCTCAAAACCTTCATCTTCCGCGTCGACCGCCACTCCAAGCCCTCGATTTTCCCCACGCACTATCACTGGTACGCCTCCGAATTCGCCGACCCGCCCCAGATCCTCCACGTGTACGACACCGTTTTCCACGGCTTCTCCGCGTCTCTCACTCCAAACGAGGTCTCCGTCATCTCCGACCACCCTTCGGTGCTTGCCGTGATAGAAGACCGTCGCCGGAGTCTCCACACTACTCGCTCACCCCAATTTTTGGGGCTCAGGAACCAGAGGGGGCTGTGGTCGGAATCGGACTATGGCTCCGATGTAATCGTCGGAGTATTTGACACTGGCGTCTGGCCGGAGCATCGGAGCTTCCAAGATAAGAATCTCGGCCCTATTCCGAGGCGGTGGAAGGGGGTTTGCGAGACAGGAACTAAATTCGCGAAGGGTAGCTGCAACAGGAAGCTGATAGGAGCTCGATTTTTCATCAAGGGCCACGAAGCGGCGGCAAATGCCGGCGGTCCGATATCAGCGATCAACGACACGGTGGAGTACCGGTCGCCGAGAGACGCTGACGGCCATGGCACCCATACCGCCTCCACGGCGATTGGCCGGTACGCGTTCGACGCCAGCATGTCGGGCTACGCCGCCGGAATCGCGAAAGGAGTTGCACCGAAAGCTCGGTTGGCGGTTTATAAGGTATGCTGGAAAGATTCCGGTTGCTTCGACTCCGATATATTGGCCGCCTATGACGCCGCTGTTAATGACGGCGTTGATGTCATTTCCATTTCAATTGGAGGCGCCGACGGCGTTTCTTCTCCGTATTATCTCGATCCGATTGCAATTGGGTCGTACGGTGCCGTTTCGCACGGAGTGTTCGTGTCGTCCTCGGCAGGAAATGACGGTCCAAACGGCATGTCGGTGTCGAATCTCGCGCCTTGGCTTATGACGGTGGGCGCGGGAACCATCGATCGGAATTTCCCGGCGGCTGTGATTTTAGGTAGCGGGCGGAGGCTAAGCGGCGTGTCGTTGTACACCGGATTACCTCTGAAAGGCAAGATGTACCCTGTCGTTTATCCCGGGAAATCAGGCATGCTCTCTGCTTCTCTGTGCATGGAGAACTCCCTGGACCCGCACCAGGTCAGGGGCAAGATTGTAATATGCGACCGCGGGAGCAGTCAGCGAGTGGCCAAGGGCTTAGTCGTGAAGAAAGCCGGCGGTGTTGGAATGATCCTCGCTAACGGAATTTCTAACGGCGAGGGTCTCATCTGCGACGCCCATATCATTGCTGCTTGCGCCGTCGGGGCCAACGAGGGTGACGCCGTTAAGGCCTACGTGGCGTCCACCAAGACTCCCACAGCGACGCTCGATTTCCAAGGGACTGTGATTGGAATAAAACCGGCTCCGGTTGTGGCTTCGTTTTCGGGTCGTGGGCCGAATGGGTTGGACCCGGAGATTCTGAAGCCGGATATAATCGCCCCGGGAGTGAACATCCTAGCCGCTTGGACCGACGCCGTGGGACCCACCGGTTTGGACACGGACAAGAGGAAAACGGAGTTTAACATTCTGTCCGGTACATCCATGGCGGCGCCGCACGTGAGCGGAGCCGCGGCTTTGCTGAAGTCGGCTCACCCTGATTGGAGCCCAGCCGCCATAAGATCCGCCATGATGACGACGGCGAGCGTGACTGATAACAGAAATCAAACCATGACTGACGAGGCAACTGGAAAGCCCTCCACGCCTTATGATATGGGGGCGGGTCATTTAAATTTGGGCCGGGCTATGGATCCTGGGCTTATTTATGACATCACCAATGATGATTACGTTAAGTTTCTCTGTGGGGTTGGGTACGGACCCAAAGTGATTCAGGTCATCACTCGGTCCGCACAAAACTGTCCGGCGAAGAAGCCTGCGCCGGAGAATCTCAATTACCCGTCGATTGGCGCAGTGTTCCCCACGGCGGGTAAGTCGAGTAAGATGTTTATTCGGACCGTGACGAATGTGGGGCAGCCCAATGCGGTGTATCGAGCCAGAATTGAGGCCCCAAAAGGGGTGACGGTGACAGTGAAGCCGTCGAGGTTGGCGTTTAGTGGGGCGGTGAAGAAGCGGAGCTTTGTGGTGACAATTGCGGTGGATAGGAGGACTGTGGTGTTTGGCGAGTCAGGTGCGGCGTTCGGGTCGCTTTATTGGACTGACGGGAAGCACGCGGTTCGGAGCCCCATTGTAGTGACCCAAATGGATCCCTTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I9 PA Peptidase_S8

Protein Analysis

786

Amino Acids

83.24

Weight (kDa)

9.12

Isoelectric Point (pI)

34.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I9	PF05922	49 - 119	1.2e-11	Peptidase inhibitor I9
Peptidase_S8	PF00082	142 - 611	1.9e-49	Subtilase family
PA	PF02225	391 - 477	5.6e-11	PA domain
fn3_6	PF17766	677 - 779	6.1e-30	Fibronectin type-III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004305)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G34980
fragaria_vesca	FvH4_2g01410 FvH4_3g19440
malus_domestica	MD05G1120100.v1.1 MD10G1123500.v1.1
prunus_persica	Prupe.8G164000_v2.0.a1
pyrus_communis	pycom05g11390 pycom10g10580
rosa_chinensis	RchiOBHm_Chr6g0244021 RchiOBHm_Chr6g0252351 RchiOBHm_Chr6g0252361
rosa_laevigata	RLG00000015390 RLG00000029339
rosa_multiflora	Rmu_co8423929.1_g000001 Rmu_sc0001251.1_g000002 Rmu_sc0006415.1_g000012 Rmu_sc0006711.1_g000006 Rmu_sc0007419.1_g000002
rosa_roxburghii	Rroxscaffold_6G00388820 Rroxscaffold_6G00388840
rosa_rugosa	Rorug05G0223500 Rorug05G0506700 Rorug05G0515900
rosa_samantha	Rh1CG388200 Rh2AG211900 Rh3DG088300 Rh5BG286100 Rh5DG294800 Rh6AG021200 Rh6CG013400 Rh6DG014700
rosa_wichuraiana	Rw6G001740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	804
AasI	GACNNNNNNGTC	1 cut(s)	458
AccB1I	GGYRCC	4 cut(s)	692, 908, 965, 1696
AccB7I	CCANNNNNTGG	1 cut(s)	379
AccBSI	CCGCTC	2 cut(s)	67, 1710
AccI	GTMKAC	1 cut(s)	156
AclWI	GGATC	8 cut(s)	226, 935, 1333, 1761, 1889, 1902, 2342, 2355
AcoI	YGGCCR	5 cut(s)	464, 685, 718, 849, 1296
AcsI	RAATTY	6 cut(s)	92, 212, 545, 1084, 1353, 1876
AcuI	CTGAAG	2 cut(s)	1589, 1748
AcvI	CACGTG	2 cut(s)	241, 1705
AcyI	GRCGYC	9 cut(s)	459, 735, 861, 909, 1383, 1428, 1448, 1620, 1697
AdeI	CACNNNGTG	1 cut(s)	982
AfaI	GTAC	9 cut(s)	203, 245, 662, 725, 962, 1142, 1172, 1684, 1958
AflIII	ACRYGT	2 cut(s)	240, 726
AgeI	ACCGGT	2 cut(s)	663, 1634
AgsI	TTSAA	2 cut(s)	91, 900
AjnI	CCWGG	3 cut(s)	1233, 1245, 1897
AjuI	GAANNNNNNNTTGG	2 cut(s)	776, 808
AluBI	AGCT	7 cut(s)	101, 480, 561, 574, 584, 788, 2220
AluI	AGCT	7 cut(s)	101, 480, 561, 574, 584, 788, 2220
Alw21I	GWGCWC	1 cut(s)	586
Alw26I	GTCTC	6 cut(s)	279, 298, 356, 527, 669, 1376
AlwI	GGATC	8 cut(s)	226, 935, 1333, 1761, 1889, 1902, 2342, 2355
Ama87I	CYCGRG	2 cut(s)	1187, 1588
ApeKI	GCWGC	3 cut(s)	561, 1397, 2116
ApoI	RAATTY	6 cut(s)	92, 212, 545, 1084, 1353, 1876
ArsI	GACNNNNNNTTYG	2 cut(s)	1010, 1042
AsiGI	ACCGGT	2 cut(s)	663, 1634
AspLEI	GCGC	8 cut(s)	112, 911, 1045, 1067, 1406, 1699, 2023, 2054
AsuC2I	CCSGG	7 cut(s)	1091, 1188, 1189, 1559, 1589, 1590, 1886
AsuHPI	GGTGA	9 cut(s)	16, 360, 1436, 1730, 1909, 2173, 2179, 2218, 2239
AvaI	CYCGRG	2 cut(s)	1187, 1588
AvaII	GGWCC	9 cut(s)	629, 1013, 1237, 1555, 1614, 1628, 1961, 1989, 2097
BalI	TGGCCA	1 cut(s)	1298
BamHI	GGATCC	2 cut(s)	1894, 2347
BanI	GGYRCC	4 cut(s)	692, 908, 965, 1696
BanII	GRGCYC	4 cut(s)	387, 586, 1754, 2327
BbrPI	CACGTG	2 cut(s)	241, 1705
BbsI	GAAGAC	1 cut(s)	342
Bbv12I	GWGCWC	1 cut(s)	586
BbvI	GCAGC	3 cut(s)	548, 1384, 2128
BccI	CCATC	1 cut(s)	1082
BcgI	CGANNNNNNTGC	8 cut(s)	929, 939, 963, 973, 2255, 2289, 2300, 2334
BciT130I	CCWGG	3 cut(s)	1235, 1247, 1899
BcnI	CCSGG	7 cut(s)	1091, 1188, 1189, 1559, 1589, 1590, 1886
BcoDI	GTCTC	6 cut(s)	279, 298, 356, 527, 669, 1376
BfaI	CTAG	1 cut(s)	1604
BfoI	RGCGCY	2 cut(s)	912, 1700
BglI	GCCNNNNNGGC	4 cut(s)	710, 1412, 1436, 1445
BlpI	GCTNAGC	1 cut(s)	1124
Bme18I	GGWCC	9 cut(s)	629, 1013, 1237, 1555, 1614, 1628, 1961, 1989, 2097
BmeT110I	CYCGRG	2 cut(s)	1187, 1588
BmsI	GCATC	1 cut(s)	481
BpiI	GAAGAC	1 cut(s)	342
Bpu10I	CCTNAGC	1 cut(s)	386
Bpu1102I	GCTNAGC	1 cut(s)	1124
BpuMI	CCSGG	7 cut(s)	1091, 1188, 1189, 1559, 1589, 1590, 1886
Bsa29I	ATCGAT	1 cut(s)	1077
BsaAI	YACGTR	3 cut(s)	241, 1444, 1705
BsaBI	GATNNNNATC	1 cut(s)	492
BsaHI	GRCGYC	9 cut(s)	459, 735, 861, 909, 1383, 1428, 1448, 1620, 1697
BsaI	GGTCTC	2 cut(s)	298, 1376
BsaWI	WCCGGW	6 cut(s)	663, 824, 1145, 1513, 1634, 1679
Bse118I	RCCGGY	6 cut(s)	623, 663, 720, 1322, 1507, 1634
Bse1I	ACTGG	3 cut(s)	203, 460, 1837
Bse3DI	GCAATG	1 cut(s)	1392
Bse8I	GATNNNNATC	1 cut(s)	492
BseBI	CCWGG	3 cut(s)	1235, 1247, 1899
BseCI	ATCGAT	1 cut(s)	1077
BseGI	GGATG	2 cut(s)	1599, 1685
BseJI	GATNNNNATC	1 cut(s)	492
BseMI	GCAATG	1 cut(s)	1392
BseMII	CTCAG	2 cut(s)	111, 400
BseNI	ACTGG	3 cut(s)	203, 460, 1837
BseRI	GAGGAG	4 cut(s)	45, 50, 59, 68
BseXI	GCAGC	3 cut(s)	548, 1384, 2128
BseYI	CCCAGC	1 cut(s)	1753
Bsh1285I	CGRYCG	4 cut(s)	160, 668, 1081, 1276
BshNI	GGYRCC	4 cut(s)	692, 908, 965, 1696
BshTI	ACCGGT	2 cut(s)	663, 1634
BshVI	ATCGAT	1 cut(s)	1077
BsiEI	CGRYCG	4 cut(s)	160, 668, 1081, 1276
BsiHKAI	GWGCWC	1 cut(s)	586
BsiHKCI	CYCGRG	2 cut(s)	1187, 1588
BsiWI	CGTACG	1 cut(s)	960
BslFI	GGGAC	2 cut(s)	1501, 1641
BsmAI	GTCTC	6 cut(s)	279, 298, 356, 527, 669, 1376
BsmBI	CGTCTC	2 cut(s)	279, 669
BsmFI	GGGAC	2 cut(s)	1501, 1641
Bso31I	GGTCTC	2 cut(s)	298, 1376
BsoBI	CYCGRG	2 cut(s)	1187, 1588
Bsp1286I	GDGCHC	4 cut(s)	387, 586, 1754, 2327
Bsp1407I	TGTACA	1 cut(s)	1140
Bsp143I	GATC	9 cut(s)	124, 231, 642, 940, 1078, 1338, 1766, 1894, 2347
Bsp1720I	GCTNAGC	1 cut(s)	1124
Bsp19I	CCATGG	2 cut(s)	688, 1689
Bsp68I	TCGCGA	2 cut(s)	551, 767
BspCNI	CTCAG	2 cut(s)	110, 399
BspDI	ATCGAT	1 cut(s)	1077
BspPI	GGATC	8 cut(s)	226, 935, 1333, 1761, 1889, 1902, 2342, 2355
BspT107I	GGYRCC	4 cut(s)	692, 908, 965, 1696
BspTNI	GGTCTC	2 cut(s)	298, 1376
BsrBI	CCGCTC	2 cut(s)	67, 1710
BsrDI	GCAATG	1 cut(s)	1392
BsrFI	RCCGGY	6 cut(s)	623, 663, 720, 1322, 1507, 1634
BsrGI	TGTACA	1 cut(s)	1140
BsrI	ACTGG	3 cut(s)	203, 460, 1837
BssAI	RCCGGY	6 cut(s)	623, 663, 720, 1322, 1507, 1634
BssMI	GATC	9 cut(s)	124, 231, 642, 940, 1078, 1338, 1766, 1894, 2347
BssNI	GRCGYC	9 cut(s)	459, 735, 861, 909, 1383, 1428, 1448, 1620, 1697
BssT1I	CCWWGG	5 cut(s)	688, 1046, 1299, 1483, 1689
Bst2UI	CCWGG	3 cut(s)	1235, 1247, 1899
Bst6I	CTCTTC	1 cut(s)	77
BstACI	GRCGYC	9 cut(s)	459, 735, 861, 909, 1383, 1428, 1448, 1620, 1697
BstAUI	TGTACA	1 cut(s)	1140
BstBAI	YACGTR	3 cut(s)	241, 1444, 1705
BstDEI	CTNAG	4 cut(s)	97, 386, 1124, 1306
BstDSI	CCRYGG	8 cut(s)	259, 688, 708, 1276, 1623, 1689, 1716, 2064
BstENI	CCTNNNNNAGG	1 cut(s)	1158
BstF5I	GGATG	2 cut(s)	1599, 1685
BstH2I	RGCGCY	2 cut(s)	912, 1700
BstHHI	GCGC	8 cut(s)	112, 911, 1045, 1067, 1406, 1699, 2023, 2054
BstKTI	GATC	9 cut(s)	127, 234, 645, 943, 1081, 1341, 1769, 1897, 2350
BstMAI	GTCTC	6 cut(s)	279, 298, 356, 527, 669, 1376
BstMBI	GATC	9 cut(s)	124, 231, 642, 940, 1078, 1338, 1766, 1894, 2347
BstMCI	CGRYCG	4 cut(s)	160, 668, 1081, 1276
BstNI	CCWGG	3 cut(s)	1235, 1247, 1899
BstNSI	RCATGY	3 cut(s)	745, 1027, 1204
BstV1I	GCAGC	3 cut(s)	548, 1384, 2128
BstV2I	GAAGAC	1 cut(s)	342
BstX2I	RGATCY	4 cut(s)	231, 1766, 1894, 2347
BstXI	CCANNNNNNTGG	1 cut(s)	1639
BstYI	RGATCY	4 cut(s)	231, 1766, 1894, 2347
Bsu15I	ATCGAT	1 cut(s)	1077
BsuTUI	ATCGAT	1 cut(s)	1077
BtgI	CCRYGG	8 cut(s)	259, 688, 708, 1276, 1623, 1689, 1716, 2064
BtsCI	GGATG	2 cut(s)	1599, 1685
BtsI	GCAGTG	1 cut(s)	2061
BtsIMutI	CAGTG	4 cut(s)	196, 453, 2061, 2178
BtuMI	TCGCGA	2 cut(s)	551, 767
CfoI	GCGC	8 cut(s)	112, 911, 1045, 1067, 1406, 1699, 2023, 2054
Cfr10I	RCCGGY	6 cut(s)	623, 663, 720, 1322, 1507, 1634
Cfr42I	CCGCGG	2 cut(s)	1279, 1719
Cfr9I	CCCGGG	2 cut(s)	1187, 1588
ClaI	ATCGAT	1 cut(s)	1077
CpoI	CGGWCCG	3 cut(s)	629, 1989, 2097
CsiI	ACCWGGT	1 cut(s)	1245
Csp6I	GTAC	9 cut(s)	202, 244, 661, 724, 961, 1141, 1171, 1683, 1957
CspAI	ACCGGT	2 cut(s)	663, 1634
CspCI	CAANNNNNGTGG	4 cut(s)	697, 732, 2220, 2255
CspI	CGGWCCG	3 cut(s)	629, 1989, 2097
CviAII	CATG	8 cut(s)	689, 742, 1024, 1201, 1222, 1690, 1774, 1816
CviQI	GTAC	9 cut(s)	202, 244, 661, 724, 961, 1141, 1171, 1683, 1957
DdeI	CTNAG	4 cut(s)	97, 386, 1124, 1306
DinI	GGCGCC	2 cut(s)	910, 1698
DpnI	GATC	9 cut(s)	126, 233, 644, 942, 1080, 1340, 1768, 1896, 2349
DpnII	GATC	9 cut(s)	124, 231, 642, 940, 1078, 1338, 1766, 1894, 2347
DraI	TTTAAA	1 cut(s)	1875
DraIII	CACNNNGTG	1 cut(s)	982
DrdI	GACNNNNNNGTC	1 cut(s)	458
DseDI	GACNNNNNNGTC	1 cut(s)	458
EaeI	YGGCCR	5 cut(s)	464, 685, 718, 849, 1296
Eam1104I	CTCTTC	1 cut(s)	77
EarI	CTCTTC	1 cut(s)	77
EciI	GGCGGA	2 cut(s)	1132, 1759
Ecl136II	GAGCTC	1 cut(s)	584
Eco130I	CCWWGG	5 cut(s)	688, 1046, 1299, 1483, 1689
Eco147I	AGGCCT	1 cut(s)	1439
Eco24I	GRGCYC	4 cut(s)	387, 586, 1754, 2327
Eco31I	GGTCTC	2 cut(s)	298, 1376
Eco32I	GATATC	1 cut(s)	636
Eco47I	GGWCC	9 cut(s)	629, 1013, 1237, 1555, 1614, 1628, 1961, 1989, 2097
Eco53kI	GAGCTC	1 cut(s)	584
Eco57I	CTGAAG	2 cut(s)	1589, 1748
Eco72I	CACGTG	2 cut(s)	241, 1705
Eco88I	CYCGRG	2 cut(s)	1187, 1588
EcoICRI	GAGCTC	1 cut(s)	584
EcoNI	CCTNNNNNAGG	1 cut(s)	1158
EcoO109I	RGGNCCY	2 cut(s)	1628, 2149
EcoRI	GAATTC	1 cut(s)	212
EcoRII	CCWGG	3 cut(s)	1233, 1245, 1897
EcoRV	GATATC	1 cut(s)	636
EcoT14I	CCWWGG	5 cut(s)	688, 1046, 1299, 1483, 1689
EcoT38I	GRGCYC	4 cut(s)	387, 586, 1754, 2327
EgeI	GGCGCC	2 cut(s)	910, 1698
EheI	GGCGCC	2 cut(s)	910, 1698
ErhI	CCWWGG	5 cut(s)	688, 1046, 1299, 1483, 1689
Esp3I	CGTCTC	2 cut(s)	279, 669
FaeI	CATG	8 cut(s)	692, 745, 1027, 1204, 1225, 1693, 1777, 1819
FalI	AAGNNNNNCTT	2 cut(s)	2204, 2236
FaqI	GGGAC	2 cut(s)	1501, 1641
FatI	CATG	8 cut(s)	688, 741, 1023, 1200, 1221, 1689, 1773, 1815
FauI	CCCGC	8 cut(s)	72, 231, 1060, 1106, 1248, 1271, 1858, 2062
FblI	GTMKAC	1 cut(s)	156
FokI	GGATG	2 cut(s)	1586, 1672
FriOI	GRGCYC	4 cut(s)	387, 586, 1754, 2327
FspBI	CTAG	1 cut(s)	1604
GlaI	GCGC	8 cut(s)	111, 910, 1044, 1066, 1405, 1698, 2022, 2053
GsaI	CCCAGC	1 cut(s)	1757
HaeII	RGCGCY	2 cut(s)	912, 1700
HhaI	GCGC	8 cut(s)	112, 911, 1045, 1067, 1406, 1699, 2023, 2054
Hin1I	GRCGYC	9 cut(s)	459, 735, 861, 909, 1383, 1428, 1448, 1620, 1697
Hin1II	CATG	8 cut(s)	692, 745, 1027, 1204, 1225, 1693, 1777, 1819
Hin6I	GCGC	8 cut(s)	110, 909, 1043, 1065, 1404, 1697, 2021, 2052
HinP1I	GCGC	8 cut(s)	110, 909, 1043, 1065, 1404, 1697, 2021, 2052
HincII	GTYRAC	1 cut(s)	157
HindII	GTYRAC	1 cut(s)	157
HphI	GGTGA	9 cut(s)	16, 360, 1436, 1730, 1909, 2173, 2179, 2218, 2239
Hpy166II	GTNNAC	5 cut(s)	157, 244, 1143, 1452, 1597
Hpy188III	TCNNGA	5 cut(s)	388, 550, 766, 938, 1312
Hpy8I	GTNNAC	5 cut(s)	157, 244, 1143, 1452, 1597
HpyAV	CCTTC	5 cut(s)	19, 151, 324, 514, 547
HpyCH4IV	ACGT	4 cut(s)	240, 1443, 1704, 1932
HpyCH4V	TGCA	5 cut(s)	4, 564, 779, 950, 1221
HpyF3I	CTNAG	4 cut(s)	97, 386, 1124, 1306
HpySE526I	ACGT	4 cut(s)	240, 1443, 1704, 1932
Hsp92I	GRCGYC	9 cut(s)	459, 735, 861, 909, 1383, 1428, 1448, 1620, 1697
Hsp92II	CATG	8 cut(s)	692, 745, 1027, 1204, 1225, 1693, 1777, 1819
HspAI	GCGC	8 cut(s)	110, 909, 1043, 1065, 1404, 1697, 2021, 2052
KasI	GGCGCC	2 cut(s)	908, 1696
KflI	GGGWCCC	1 cut(s)	1628
KroI	GCCGGC	2 cut(s)	623, 1322
KroNI	GCCGGC	2 cut(s)	625, 1324
KspI	CCGCGG	2 cut(s)	1279, 1719
Kzo9I	GATC	9 cut(s)	124, 231, 642, 940, 1078, 1338, 1766, 1894, 2347
Lsp1109I	GCAGC	3 cut(s)	548, 1384, 2128
LweI	GCATC	1 cut(s)	481
MabI	ACCWGGT	1 cut(s)	1245
MaeI	CTAG	1 cut(s)	1604
MaeII	ACGT	4 cut(s)	240, 1443, 1704, 1932
MaeIII	GTNAC	7 cut(s)	1424, 1792, 2101, 2161, 2167, 2227, 2335
MalI	GATC	9 cut(s)	126, 233, 644, 942, 1080, 1340, 1768, 1896, 2349
MbiI	CCGCTC	2 cut(s)	67, 1710
MboI	GATC	9 cut(s)	124, 231, 642, 940, 1078, 1338, 1766, 1894, 2347
MboII	GAAGA	8 cut(s)	64, 79, 139, 347, 915, 1327, 2023, 2221
MfeI	CAATTG	3 cut(s)	900, 951, 2232
MflI	RGATCY	4 cut(s)	231, 1766, 1894, 2347
MhlI	GDGCHC	4 cut(s)	387, 586, 1754, 2327
MlsI	TGGCCA	1 cut(s)	1298
MluI	ACGCGT	1 cut(s)	726
MluNI	TGGCCA	1 cut(s)	1298
Mly113I	GGCGCC	2 cut(s)	909, 1697
MlyI	GAGTC	4 cut(s)	358, 830, 1453, 2276
MmeI	TCCRAC	5 cut(s)	330, 390, 421, 1312, 1533
Mox20I	TGGCCA	1 cut(s)	1298
MroNI	GCCGGC	2 cut(s)	623, 1322
MscI	TGGCCA	1 cut(s)	1298
MseI	TTAA	5 cut(s)	870, 1434, 1668, 1874, 1935
MslI	CAYNNNNRTG	2 cut(s)	1028, 2333
Msp20I	TGGCCA	1 cut(s)	1298
MspA1I	CMGCKG	3 cut(s)	866, 1278, 1718
MunI	CAATTG	3 cut(s)	900, 951, 2232
MvaI	CCWGG	3 cut(s)	1235, 1247, 1899
NaeI	GCCGGC	2 cut(s)	625, 1324
NarI	GGCGCC	2 cut(s)	909, 1697
NciI	CCSGG	7 cut(s)	1091, 1188, 1189, 1559, 1589, 1590, 1886
NcoI	CCATGG	2 cut(s)	688, 1689
NdeII	GATC	9 cut(s)	124, 231, 642, 940, 1078, 1338, 1766, 1894, 2347
NgoMIV	GCCGGC	2 cut(s)	623, 1322
NlaIII	CATG	8 cut(s)	692, 745, 1027, 1204, 1225, 1693, 1777, 1819
NmeAIII	GCCGAG	3 cut(s)	477, 696, 977
NmuCI	GTSAC	7 cut(s)	1424, 1792, 2101, 2161, 2167, 2227, 2335
NruI	TCGCGA	2 cut(s)	551, 767
NspI	RCATGY	3 cut(s)	745, 1027, 1204
PaeI	GCATGC	1 cut(s)	1204
PceI	AGGCCT	1 cut(s)	1439
PcsI	WCGNNNNNNNCGW	4 cut(s)	294, 984, 1416, 2189
PdiI	GCCGGC	2 cut(s)	625, 1324
PfeI	GAWTC	8 cut(s)	413, 493, 762, 821, 1036, 1564, 1972, 2029
Pfl23II	CGTACG	1 cut(s)	960
PflMI	CCANNNNNTGG	1 cut(s)	379
PinAI	ACCGGT	2 cut(s)	663, 1634
Ple19I	CGATCG	1 cut(s)	1081
PleI	GAGTC	4 cut(s)	357, 830, 1453, 2275
PluTI	GGCGCC	2 cut(s)	912, 1700
PmaCI	CACGTG	2 cut(s)	241, 1705
PmlI	CACGTG	2 cut(s)	241, 1705
PpsI	GAGTC	4 cut(s)	357, 830, 1453, 2275
Ppu21I	YACGTR	3 cut(s)	241, 1444, 1705
PpuMI	RGGWCCY	1 cut(s)	1628
PsiI	TTATAA	1 cut(s)	804
Psp124BI	GAGCTC	1 cut(s)	586
Psp5II	RGGWCCY	1 cut(s)	1628
Psp6I	CCWGG	3 cut(s)	1233, 1245, 1897
PspCI	CACGTG	2 cut(s)	241, 1705
PspFI	CCCAGC	1 cut(s)	1753
PspGI	CCWGG	3 cut(s)	1233, 1245, 1897
PspLI	CGTACG	1 cut(s)	960
PspPPI	RGGWCCY	1 cut(s)	1628
PsuI	RGATCY	4 cut(s)	231, 1766, 1894, 2347
PvuI	CGATCG	1 cut(s)	1081
RruI	TCGCGA	2 cut(s)	551, 767
RsaI	GTAC	9 cut(s)	203, 245, 662, 725, 962, 1142, 1172, 1684, 1958
RsaNI	GTAC	9 cut(s)	202, 244, 661, 724, 961, 1141, 1171, 1683, 1957
RseI	CAYNNNNRTG	2 cut(s)	1028, 2333
Rsr2I	CGGWCCG	3 cut(s)	629, 1989, 2097
RsrII	CGGWCCG	3 cut(s)	629, 1989, 2097
SacI	GAGCTC	1 cut(s)	586
SacII	CCGCGG	2 cut(s)	1279, 1719
SalI	GTCGAC	1 cut(s)	155
SaqAI	TTAA	5 cut(s)	870, 1434, 1668, 1874, 1935
Sau3AI	GATC	9 cut(s)	124, 231, 642, 940, 1078, 1338, 1766, 1894, 2347
SchI	GAGTC	4 cut(s)	358, 830, 1453, 2276
SduI	GDGCHC	4 cut(s)	387, 586, 1754, 2327
SexAI	ACCWGGT	1 cut(s)	1245
SfaNI	GCATC	1 cut(s)	481
SfoI	GGCGCC	2 cut(s)	910, 1698
Sfr303I	CCGCGG	2 cut(s)	1279, 1719
SgrBI	CCGCGG	2 cut(s)	1279, 1719
SinI	GGWCC	9 cut(s)	629, 1013, 1237, 1555, 1614, 1628, 1961, 1989, 2097
SmaI	CCCGGG	2 cut(s)	1189, 1590
SmiI	ATTTAAAT	1 cut(s)	1875
SmiMI	CAYNNNNRTG	2 cut(s)	1028, 2333
SphI	GCATGC	1 cut(s)	1204
SseBI	AGGCCT	1 cut(s)	1439
SspDI	GGCGCC	2 cut(s)	908, 1696
SspMI	CTAG	1 cut(s)	1604
SstI	GAGCTC	1 cut(s)	586
StuI	AGGCCT	1 cut(s)	1439
StyI	CCWWGG	5 cut(s)	688, 1046, 1299, 1483, 1689
SwaI	ATTTAAAT	1 cut(s)	1875
TaiI	ACGT	4 cut(s)	243, 1446, 1707, 1935
TaqII	GACCGA	2 cut(s)	1631, 1977
TatI	WGTACW	1 cut(s)	1140
TfiI	GAWTC	8 cut(s)	413, 493, 762, 821, 1036, 1564, 1972, 2029
Tru1I	TTAA	5 cut(s)	870, 1434, 1668, 1874, 1935
Tru9I	TTAA	5 cut(s)	870, 1434, 1668, 1874, 1935
TscAI	CASTG	4 cut(s)	203, 460, 2061, 2178
TseFI	GTSAC	7 cut(s)	1424, 1792, 2101, 2161, 2167, 2227, 2335
TseI	GCWGC	3 cut(s)	561, 1397, 2116
Tsp45I	GTSAC	7 cut(s)	1424, 1792, 2101, 2161, 2167, 2227, 2335
TspDTI	ATGAA	2 cut(s)	133, 583
TspGWI	ACGGA	7 cut(s)	286, 918, 993, 1365, 1661, 1676, 1974
TspMI	CCCGGG	2 cut(s)	1187, 1588
TspRI	CASTG	4 cut(s)	203, 460, 2061, 2178
Van91I	CCANNNNNTGG	1 cut(s)	379
VpaK11BI	GGWCC	9 cut(s)	629, 1013, 1237, 1555, 1614, 1628, 1961, 1989, 2097
XagI	CCTNNNNNAGG	1 cut(s)	1158
XapI	RAATTY	6 cut(s)	92, 212, 545, 1084, 1353, 1876
XceI	RCATGY	3 cut(s)	745, 1027, 1204
XmaI	CCCGGG	2 cut(s)	1187, 1588
XmiI	GTMKAC	1 cut(s)	156
XspI	CTAG	1 cut(s)	1604

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.