Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr6

Orthogroup ID

OG0004305

Physical Location & Seq

Genomic Coordinates Reverse (-)

2780355 .. 2782669

Gene Structure 2315 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw6G001740.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2280 bp

ATGGCTACCTTTCTCTCCATTTCCCTCATCTTCATCTTCTTCTTCATATCTCAGATCGAATCAGAGCCCCTCGATCAGACGGTGAAGACCTTCATCTTCAGAATCGACAGATTCTCCAAGCCGTCGATTTTCCAGTCGCATTACCACTGGTACACTTCCGAATTCGCCGACCCACCTCAAATCCTCAACGTATACGACACCGTTTTCCATGGCTTCTCCGCAACTCTCAGCCCCGACCAAGTTGCCTCCCTCAGCCAACACCCCGCCGTCCTCCACGTGTTCGAAGACCGCCGCCGCCAGCTCCACACCACCCGCTCGCCGCAGTTCCTCGGCCTGAGAAACCAGAAGGGGCTCTGGTCGGAATCCGATTACGGCTCTGACGTCATCGTCGGGGTTTTCGACACTGGAGTTTGGCCCGAGCGTCGGAGCTTCTCGGATAGGAATCTCGGTCCGGTTCCGAAGCGGTGGAGAGGGGTCTGTGAAACCGGAGTTAAATTTACAGCTCGGAATTGCAATAAGAAGGTGATTGGAGCTCGGTTCTTCATCAAAGGTCATGAAGCTGCCGCCAATGCCGGCGGTCCGATAACTGCGATCAACGGTTCAGTTGAATTCCGGTCTCCTAGAGACGCCGACGGACATGGAACACACACGGCGTCCACCGCCGCCGGTCGGTACGCCTTCGAGGCCAGCATGTCCGGCTACGCTTCCGGCATCGCAAAGGGTGTTGCTCCGAAATCTCGCTTGGCTATCTACAAGGTCTGCTGGAAGGACTCCGGTTGCTTCGACTCCGATATCTTGGCCGCCTTCGACGCCGCCGTCAACGACGGCGTAGACGTCATTTCAATCTCAATTGGAGGCGGCGATGGAATTTCTTCTCCCTATTATCTCGATCCGATTGCAATCGGATCATTCGGCGCCGTTTCGCATGGAGTGTTTGTTTCGGCCTCCGCCGGGAACGACGGACCTAACGGAATGTCGGTGACGAACCTGGCGCCGTGGCTGACGACTGTCGGAGCGGGAACAATCGATCGGAACTTCCCGGCGGTGGTGGTCCTAGGCGACGGCCGGAGACTCTCCGGCGTTTCTCTATACGCCGGAGCTCCGCTGAAGGGGAAGATGTACCCGCTGGTCTATCCCGGAAAGTCAGGTATGCTCTCTACTTCACTGTGCATGGAGAACTCCCTGGACCCGCGTCAGGTGAGGGGCAAAATCGTCATTTGCGACAGGGGAAACAATCCCAGAGTGGCGAAAGGTATGGTGGTGAAGAAAGCAGGAGGTGTGGGGATGATCCTGGCTAACGGAATCTCCAACGGCGAAGGACTCGTCGGCGATGCTCACCTTCTCCCCACCGCAGCTGTCGGCGCCGACGAAGGTGACGCCGTCAAGTCTTATGTCTCCTCTACCAAGACTCCTTCAGCTACTATTGACTTCGAGGGGACTGTGATCGGAATCAAGCCGGCTCCGGTGGTTGCTTCGTTTTCGGGTAGAGGGCCAAACGGGTTGAACCCGGAGATTCTGAAGCCGGATCTGATCGCTCCGGGAGTCAACATCCTCGCTGCTTGGACCGACGCTGTGGGCCCAACCGGGTTGGAAGCGGATAACCGGAAAACGGAGTTTAATATCCTCTCGGGAACATCAATGGCTGCCCCTCACGTGAGCGGAGCCGCAGCTCTGCTGAAATCGGCGATGATGACGACTGCTGGCATCACCAATAATCAGAACAAGACCATGACTGATGAAGCTACTGGAAACCCCTCCACTCCTTATGATTTAGGGGCGGGTCATTTGAATTTGGAGCGGGCTATGGACCCGGGTCTGGTATATGATGTCACCGGCGAGGATTACGTCCAGTTTCTCTGCTCGGTCGGGTACGGGCCGAGAGTAATCCAGGTGATCACTCGGTCGCCGCCGAAATGTCCGGGGAAAAAGACTTCGCCGGGAAATCTGAACTACCCGTCAATCGCCGCGTTGTTTCCGACTTCCGCGTCGGGGCCGTCCAGCAAGACGTTTATTCGGACGGTGACGAATGTGGGCCAGCCCAATGCGGTATACAGGCCCATGATAGAGGCGCCTAAAGGAGTGAAGGTGATGGTGAAGCCGTCGAAGCTGGTGTTCACAGAGGCGGTGAAGAAGCGGAGCTACTTGGTGACGGTGGCGGTGGATAGGAGCAACATCGTGTTGGGTGAGTCGGGGGGTGTGTTCGGGTCGCTATATTGGAGCGATGGGAAGCAGCATGTGGTTCGGAGCCCCATTGTGGTGACCCAAATGGATCCCTTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I9 PA Peptidase_S8

Protein Analysis

759

Amino Acids

80.49

Weight (kDa)

8.91

Isoelectric Point (pI)

35.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I9	PF05922	39 - 102	8.7e-13	Peptidase inhibitor I9
Peptidase_S8	PF00082	125 - 576	3.6e-39	Subtilase family
PA	PF02225	374 - 460	2e-13	PA domain
fn3_6	PF17766	648 - 753	2.6e-27	Fibronectin type-III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0004305)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G34980
fragaria_vesca	FvH4_2g01410 FvH4_3g19440
malus_domestica	MD05G1120100.v1.1 MD10G1123500.v1.1
prunus_persica	Prupe.8G164000_v2.0.a1
pyrus_communis	pycom05g11390 pycom10g10580
rosa_chinensis	RchiOBHm_Chr6g0244021 RchiOBHm_Chr6g0252351 RchiOBHm_Chr6g0252361
rosa_laevigata	RLG00000015390 RLG00000029339
rosa_multiflora	Rmu_co8423929.1_g000001 Rmu_sc0001251.1_g000002 Rmu_sc0006415.1_g000012 Rmu_sc0006711.1_g000006 Rmu_sc0007419.1_g000002
rosa_roxburghii	Rroxscaffold_6G00388820 Rroxscaffold_6G00388840
rosa_rugosa	Rorug05G0223500 Rorug05G0506700 Rorug05G0515900
rosa_samantha	Rh1CG388200 Rh2AG211900 Rh3DG088300 Rh5BG286100 Rh5DG294800 Rh6AG021200 Rh6CG013400 Rh6DG014700
rosa_wichuraiana	Rw6G001740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	3 cut(s)	386, 815, 1984
AatII	GACGTC	2 cut(s)	384, 837
AccB1I	GGYRCC	4 cut(s)	914, 991, 1361, 2068
AccBSI	CCGCTC	4 cut(s)	315, 1016, 1659, 1798
AccI	GTMKAC	3 cut(s)	192, 831, 2049
AccII	CGCG	3 cut(s)	1192, 1967, 1985
AclWI	GGATC	6 cut(s)	884, 913, 1282, 1533, 2264, 2277
AcoI	YGGCCR	2 cut(s)	798, 1063
AcsI	RAATTY	5 cut(s)	161, 494, 608, 867, 1789
AcuI	CTGAAG	4 cut(s)	82, 1127, 1398, 1538
AcvI	CACGTG	2 cut(s)	277, 1654
AfaI	GTAC	4 cut(s)	152, 674, 1121, 1871
AflIII	ACRYGT	1 cut(s)	276
AgsI	TTSAA	4 cut(s)	608, 843, 1504, 1789
AjnI	CCWGG	4 cut(s)	987, 1182, 1290, 1887
AjuI	GAANNNNNNNTTGG	2 cut(s)	725, 757
Alw21I	GWGCWC	2 cut(s)	535, 1102
Alw26I	GTCTC	4 cut(s)	618, 621, 1063, 1399
AlwI	GGATC	6 cut(s)	884, 913, 1282, 1533, 2264, 2277
Ama87I	CYCGRG	3 cut(s)	416, 1627, 1810
ApaI	GGGCCC	1 cut(s)	1580
ApeKI	GCWGC	6 cut(s)	560, 1352, 1556, 1643, 1667, 2230
ApoI	RAATTY	5 cut(s)	161, 494, 608, 867, 1789
ArsI	GACNNNNNNTTYG	2 cut(s)	1308, 1340
Asp700I	GAANNNNTTC	2 cut(s)	89, 871
AspA2I	CCTAGG	1 cut(s)	1054
AspLEI	GCGC	4 cut(s)	917, 994, 1364, 2071
AsuII	TTCGAA	1 cut(s)	282
AvaI	CYCGRG	3 cut(s)	416, 1627, 1810
AvaII	GGWCC	7 cut(s)	449, 578, 962, 1051, 1186, 1563, 1807
AvrII	CCTAGG	1 cut(s)	1054
BaeGI	GKGCMC	1 cut(s)	1580
BamHI	GGATCC	1 cut(s)	2269
BanI	GGYRCC	4 cut(s)	914, 991, 1361, 2068
BanII	GRGCYC	6 cut(s)	69, 354, 535, 1102, 1580, 2249
BarI	GAAGNNNNNNTAC	2 cut(s)	2123, 2155
BbrPI	CACGTG	2 cut(s)	277, 1654
BbsI	GAAGAC	2 cut(s)	92, 291
Bbv12I	GWGCWC	2 cut(s)	535, 1102
BbvCI	CCTCAGC	1 cut(s)	251
BbvI	GCAGC	6 cut(s)	547, 1364, 1543, 1630, 1679, 2242
BccI	CCATC	3 cut(s)	857, 2083, 2216
BcgI	CGANNNNNNTGC	6 cut(s)	224, 258, 878, 912, 2222, 2256
BciT130I	CCWGG	4 cut(s)	989, 1184, 1292, 1889
BclI	TGATCA	1 cut(s)	1893
BcoDI	GTCTC	4 cut(s)	618, 621, 1063, 1399
BfaI	CTAG	2 cut(s)	621, 1055
BfoI	RGCGCY	4 cut(s)	918, 995, 1365, 2072
BglI	GCCNNNNNGGC	1 cut(s)	683
BlnI	CCTAGG	1 cut(s)	1054
Bme18I	GGWCC	7 cut(s)	449, 578, 962, 1051, 1186, 1563, 1807
BmeT110I	CYCGRG	3 cut(s)	416, 1627, 1810
BmsI	GCATC	3 cut(s)	720, 1321, 1713
BoxI	GACNNNNGTC	3 cut(s)	1007, 1191, 1812
BpiI	GAAGAC	2 cut(s)	92, 291
BpmI	CTGGAG	1 cut(s)	426
Bpu10I	CCTNAGC	1 cut(s)	251
Bpu14I	TTCGAA	1 cut(s)	282
Bsa29I	ATCGAT	1 cut(s)	1026
BsaAI	YACGTR	2 cut(s)	277, 1654
BsaBI	GATNNNNATC	3 cut(s)	441, 899, 1448
BsaI	GGTCTC	1 cut(s)	621
BsaJI	CCNNGG	7 cut(s)	208, 328, 995, 1054, 1182, 1810, 1919
BsaWI	WCCGGW	6 cut(s)	451, 485, 612, 773, 1462, 1602
Bse118I	RCCGGY	4 cut(s)	572, 665, 1456, 1832
Bse1I	ACTGG	5 cut(s)	133, 152, 409, 1750, 1849
Bse8I	GATNNNNATC	3 cut(s)	441, 899, 1448
BseBI	CCWGG	4 cut(s)	989, 1184, 1292, 1889
BseCI	ATCGAT	1 cut(s)	1026
BseDI	CCNNGG	7 cut(s)	208, 328, 995, 1054, 1182, 1810, 1919
BseGI	GGATG	2 cut(s)	1290, 1548
BseJI	GATNNNNATC	3 cut(s)	441, 899, 1448
BseMII	CTCAG	4 cut(s)	65, 241, 265, 326
BseNI	ACTGG	5 cut(s)	133, 152, 409, 1750, 1849
BseRI	GAGGAG	1 cut(s)	1387
BseSI	GKGCMC	1 cut(s)	1580
BseX3I	CGGCCG	1 cut(s)	1063
BseXI	GCAGC	6 cut(s)	547, 1364, 1543, 1630, 1679, 2242
Bsh1236I	CGCG	3 cut(s)	1192, 1967, 1985
Bsh1285I	CGRYCG	5 cut(s)	670, 1030, 1066, 1866, 1904
BshNI	GGYRCC	4 cut(s)	914, 991, 1361, 2068
BshVI	ATCGAT	1 cut(s)	1026
BsiEI	CGRYCG	5 cut(s)	670, 1030, 1066, 1866, 1904
BsiHKAI	GWGCWC	2 cut(s)	535, 1102
BsiHKCI	CYCGRG	3 cut(s)	416, 1627, 1810
BslFI	GGGAC	1 cut(s)	1450
BsmAI	GTCTC	4 cut(s)	618, 621, 1063, 1399
BsmBI	CGTCTC	1 cut(s)	618
BsmFI	GGGAC	1 cut(s)	1450
Bso31I	GGTCTC	1 cut(s)	621
BsoBI	CYCGRG	3 cut(s)	416, 1627, 1810
Bsp119I	TTCGAA	1 cut(s)	282
Bsp120I	GGGCCC	1 cut(s)	1576
Bsp1286I	GDGCHC	6 cut(s)	69, 354, 535, 1102, 1580, 2249
Bsp19I	CCATGG	1 cut(s)	208
BspCNI	CTCAG	4 cut(s)	64, 240, 264, 327
BspDI	ATCGAT	1 cut(s)	1026
BspFNI	CGCG	3 cut(s)	1192, 1967, 1985
BspHI	TCATGA	1 cut(s)	553
BspPI	GGATC	6 cut(s)	884, 913, 1282, 1533, 2264, 2277
BspT104I	TTCGAA	1 cut(s)	282
BspT107I	GGYRCC	4 cut(s)	914, 991, 1361, 2068
BspTNI	GGTCTC	1 cut(s)	621
BsrBI	CCGCTC	4 cut(s)	315, 1016, 1659, 1798
BsrFI	RCCGGY	4 cut(s)	572, 665, 1456, 1832
BsrI	ACTGG	5 cut(s)	133, 152, 409, 1750, 1849
BssAI	RCCGGY	4 cut(s)	572, 665, 1456, 1832
BssECI	CCNNGG	7 cut(s)	208, 328, 995, 1054, 1182, 1810, 1919
BssNAI	GTATAC	2 cut(s)	193, 2050
BssT1I	CCWWGG	2 cut(s)	208, 1054
Bst1107I	GTATAC	2 cut(s)	193, 2050
Bst2UI	CCWGG	4 cut(s)	989, 1184, 1292, 1889
Bst4CI	ACNGT	8 cut(s)	82, 202, 599, 1009, 1167, 1441, 2020, 2152
BstBAI	YACGTR	2 cut(s)	277, 1654
BstBI	TTCGAA	1 cut(s)	282
BstC8I	GCNNGC	8 cut(s)	299, 317, 574, 688, 1458, 1702, 1800, 2036
BstDEI	CTNAG	4 cut(s)	51, 227, 251, 335
BstDSI	CCRYGG	2 cut(s)	208, 995
BstEII	GGTNACC	1 cut(s)	2257
BstF5I	GGATG	2 cut(s)	1290, 1548
BstFNI	CGCG	3 cut(s)	1192, 1967, 1985
BstH2I	RGCGCY	4 cut(s)	918, 995, 1365, 2072
BstHHI	GCGC	4 cut(s)	917, 994, 1364, 2071
BstMAI	GTCTC	4 cut(s)	618, 621, 1063, 1399
BstMCI	CGRYCG	5 cut(s)	670, 1030, 1066, 1866, 1904
BstMWI	GCNNNNNNNGC	7 cut(s)	569, 659, 683, 696, 809, 1361, 2104
BstNI	CCWGG	4 cut(s)	989, 1184, 1292, 1889
BstNSI	RCATGY	2 cut(s)	694, 2237
BstPAI	GACNNNNGTC	3 cut(s)	1007, 1191, 1812
BstPI	GGTNACC	1 cut(s)	2257
BstSLI	GKGCMC	1 cut(s)	1580
BstUI	CGCG	3 cut(s)	1192, 1967, 1985
BstV1I	GCAGC	6 cut(s)	547, 1364, 1543, 1630, 1679, 2242
BstV2I	GAAGAC	2 cut(s)	92, 291
BstX2I	RGATCY	2 cut(s)	1525, 2269
BstYI	RGATCY	2 cut(s)	1525, 2269
BstZ17I	GTATAC	2 cut(s)	193, 2050
BstZI	CGGCCG	1 cut(s)	1063
Bsu15I	ATCGAT	1 cut(s)	1026
BsuTUI	ATCGAT	1 cut(s)	1026
BtgI	CCRYGG	2 cut(s)	208, 995
BtgZI	GCGATG	5 cut(s)	697, 876, 1344, 1700, 2235
BtsCI	GGATG	2 cut(s)	1290, 1548
BtsIMutI	CAGTG	3 cut(s)	145, 402, 1163
Cac8I	GCNNGC	8 cut(s)	299, 317, 574, 688, 1458, 1702, 1800, 2036
CciI	TCATGA	1 cut(s)	553
CfoI	GCGC	4 cut(s)	917, 994, 1364, 2071
Cfr10I	RCCGGY	4 cut(s)	572, 665, 1456, 1832
Cfr9I	CCCGGG	1 cut(s)	1810
ClaI	ATCGAT	1 cut(s)	1026
CpoI	CGGWCCG	2 cut(s)	449, 578
CseI	GACGC	8 cut(s)	410, 635, 642, 818, 1181, 1385, 1577, 1974
Csp6I	GTAC	4 cut(s)	151, 673, 1120, 1870
CspI	CGGWCCG	2 cut(s)	449, 578
CviAII	CATG	9 cut(s)	209, 554, 638, 691, 926, 1171, 1729, 2059, 2234
CviQI	GTAC	4 cut(s)	151, 673, 1120, 1870
DdeI	CTNAG	4 cut(s)	51, 227, 251, 335
DinI	GGCGCC	4 cut(s)	916, 993, 1363, 2070
DrdI	GACNNNNNNGTC	3 cut(s)	386, 815, 1984
DseDI	GACNNNNNNGTC	3 cut(s)	386, 815, 1984
EaeI	YGGCCR	2 cut(s)	798, 1063
EagI	CGGCCG	1 cut(s)	1063
EciI	GGCGGA	1 cut(s)	937
Ecl136II	GAGCTC	2 cut(s)	533, 1100
EclXI	CGGCCG	1 cut(s)	1063
Eco130I	CCWWGG	2 cut(s)	208, 1054
Eco24I	GRGCYC	6 cut(s)	69, 354, 535, 1102, 1580, 2249
Eco31I	GGTCTC	1 cut(s)	621
Eco32I	GATATC	1 cut(s)	793
Eco47I	GGWCC	7 cut(s)	449, 578, 962, 1051, 1186, 1563, 1807
Eco52I	CGGCCG	1 cut(s)	1063
Eco53kI	GAGCTC	2 cut(s)	533, 1100
Eco57I	CTGAAG	4 cut(s)	82, 1127, 1398, 1538
Eco72I	CACGTG	2 cut(s)	277, 1654
Eco88I	CYCGRG	3 cut(s)	416, 1627, 1810
Eco91I	GGTNACC	1 cut(s)	2257
EcoICRI	GAGCTC	2 cut(s)	533, 1100
EcoO65I	GGTNACC	1 cut(s)	2257
EcoRI	GAATTC	2 cut(s)	161, 608
EcoRII	CCWGG	4 cut(s)	987, 1182, 1290, 1887
EcoRV	GATATC	1 cut(s)	793
EcoT14I	CCWWGG	2 cut(s)	208, 1054
EcoT38I	GRGCYC	6 cut(s)	69, 354, 535, 1102, 1580, 2249
EgeI	GGCGCC	4 cut(s)	916, 993, 1363, 2070
EheI	GGCGCC	4 cut(s)	916, 993, 1363, 2070
ErhI	CCWWGG	2 cut(s)	208, 1054
Esp3I	CGTCTC	1 cut(s)	618
FaeI	CATG	9 cut(s)	212, 557, 641, 694, 929, 1174, 1732, 2062, 2237
FaqI	GGGAC	1 cut(s)	1450
FatI	CATG	9 cut(s)	208, 553, 637, 690, 925, 1170, 1728, 2058, 2233
FauI	CCCGC	7 cut(s)	271, 320, 1009, 1131, 1197, 1771, 1791
FbaI	TGATCA	1 cut(s)	1893
FblI	GTMKAC	3 cut(s)	192, 831, 2049
FokI	GGATG	2 cut(s)	1297, 1535
FriOI	GRGCYC	6 cut(s)	69, 354, 535, 1102, 1580, 2249
FspBI	CTAG	2 cut(s)	621, 1055
GlaI	GCGC	4 cut(s)	916, 993, 1363, 2070
GsuI	CTGGAG	1 cut(s)	426
HaeII	RGCGCY	4 cut(s)	918, 995, 1365, 2072
HgaI	GACGC	8 cut(s)	410, 635, 642, 818, 1181, 1385, 1577, 1974
HhaI	GCGC	4 cut(s)	917, 994, 1364, 2071
Hin1II	CATG	9 cut(s)	212, 557, 641, 694, 929, 1174, 1732, 2062, 2237
Hin6I	GCGC	4 cut(s)	915, 992, 1362, 2069
HinP1I	GCGC	4 cut(s)	915, 992, 1362, 2069
HincII	GTYRAC	2 cut(s)	820, 1546
HindII	GTYRAC	2 cut(s)	820, 1546
Hpy166II	GTNNAC	8 cut(s)	153, 193, 657, 820, 832, 1546, 2050, 2115
Hpy188III	TCNNGA	3 cut(s)	554, 887, 1629
Hpy8I	GTNNAC	8 cut(s)	153, 193, 657, 820, 832, 1546, 2050, 2115
HpyCH4III	ACNGT	8 cut(s)	82, 202, 599, 1009, 1167, 1441, 2020, 2152
HpyCH4IV	ACGT	7 cut(s)	189, 276, 381, 834, 1653, 1845, 2006
HpyCH4V	TGCA	3 cut(s)	513, 899, 1170
HpyF10VI	GCNNNNNNNGC	7 cut(s)	569, 659, 683, 696, 809, 1361, 2104
HpyF3I	CTNAG	4 cut(s)	51, 227, 251, 335
HpySE526I	ACGT	7 cut(s)	189, 276, 381, 834, 1653, 1845, 2006
Hsp92II	CATG	9 cut(s)	212, 557, 641, 694, 929, 1174, 1732, 2062, 2237
HspAI	GCGC	4 cut(s)	915, 992, 1362, 2069
KasI	GGCGCC	4 cut(s)	914, 991, 1361, 2068
KroI	GCCGGC	2 cut(s)	572, 1456
KroNI	GCCGGC	2 cut(s)	574, 1458
Ksp22I	TGATCA	1 cut(s)	1893
Lsp1109I	GCAGC	6 cut(s)	547, 1364, 1543, 1630, 1679, 2242
LweI	GCATC	3 cut(s)	720, 1321, 1713
MaeI	CTAG	2 cut(s)	621, 1055
MaeII	ACGT	7 cut(s)	189, 276, 381, 834, 1653, 1845, 2006
MaeIII	GTNAC	6 cut(s)	979, 1373, 1828, 2020, 2146, 2257
MbiI	CCGCTC	4 cut(s)	315, 1016, 1659, 1798
MfeI	CAATTG	1 cut(s)	849
MflI	RGATCY	2 cut(s)	1525, 2269
MhlI	GDGCHC	6 cut(s)	69, 354, 535, 1102, 1580, 2249
MluCI	AATT	7 cut(s)	161, 494, 508, 608, 849, 867, 1789
Mly113I	GGCGCC	4 cut(s)	915, 992, 1362, 2069
MlyI	GAGTC	7 cut(s)	764, 779, 1065, 1314, 1402, 1551, 2195
MmeI	TCCRAC	6 cut(s)	339, 404, 991, 1332, 1569, 2000
MroNI	GCCGGC	2 cut(s)	572, 1456
MroXI	GAANNNNTTC	2 cut(s)	89, 871
MseI	TTAA	2 cut(s)	492, 1617
MslI	CAYNNNNRTG	1 cut(s)	2255
MspA1I	CMGCKG	3 cut(s)	1105, 1126, 1355
MunI	CAATTG	1 cut(s)	849
MvaI	CCWGG	4 cut(s)	989, 1184, 1292, 1889
MvnI	CGCG	3 cut(s)	1192, 1967, 1985
MwoI	GCNNNNNNNGC	7 cut(s)	569, 659, 683, 696, 809, 1361, 2104
NaeI	GCCGGC	2 cut(s)	574, 1458
NarI	GGCGCC	4 cut(s)	915, 992, 1362, 2069
NcoI	CCATGG	1 cut(s)	208
NgoMIV	GCCGGC	2 cut(s)	572, 1456
NlaIII	CATG	9 cut(s)	212, 557, 641, 694, 929, 1174, 1732, 2062, 2237
NmeAIII	GCCGAG	2 cut(s)	309, 1902
NmuCI	GTSAC	6 cut(s)	979, 1373, 1828, 2020, 2146, 2257
NspI	RCATGY	2 cut(s)	694, 2237
NspV	TTCGAA	1 cut(s)	282
PagI	TCATGA	1 cut(s)	553
PcsI	WCGNNNNNNNCGW	6 cut(s)	378, 396, 813, 831, 1218, 1365
PdiI	GCCGGC	2 cut(s)	574, 1458
PdmI	GAANNNNTTC	2 cut(s)	89, 871
PfeI	GAWTC	8 cut(s)	59, 102, 111, 362, 442, 1302, 1449, 1513
PflFI	GACNNNGTC	1 cut(s)	1379
PfoI	TCCNGGA	2 cut(s)	1135, 1537
Ple19I	CGATCG	1 cut(s)	1030
PleI	GAGTC	7 cut(s)	764, 779, 1065, 1314, 1402, 1550, 2194
PluTI	GGCGCC	4 cut(s)	918, 995, 1365, 2072
PmaCI	CACGTG	2 cut(s)	277, 1654
PmlI	CACGTG	2 cut(s)	277, 1654
PpsI	GAGTC	7 cut(s)	764, 779, 1065, 1314, 1402, 1550, 2194
Ppu21I	YACGTR	2 cut(s)	277, 1654
PshAI	GACNNNNGTC	3 cut(s)	1007, 1191, 1812
Psp124BI	GAGCTC	2 cut(s)	535, 1102
Psp6I	CCWGG	4 cut(s)	987, 1182, 1290, 1887
PspCI	CACGTG	2 cut(s)	277, 1654
PspEI	GGTNACC	1 cut(s)	2257
PspGI	CCWGG	4 cut(s)	987, 1182, 1290, 1887
PspOMI	GGGCCC	1 cut(s)	1576
PsuI	RGATCY	2 cut(s)	1525, 2269
PsyI	GACNNNGTC	1 cut(s)	1379
PvuI	CGATCG	1 cut(s)	1030
PvuII	CAGCTG	1 cut(s)	1355
RsaI	GTAC	4 cut(s)	152, 674, 1121, 1871
RsaNI	GTAC	4 cut(s)	151, 673, 1120, 1870
RseI	CAYNNNNRTG	1 cut(s)	2255
Rsr2I	CGGWCCG	2 cut(s)	449, 578
RsrII	CGGWCCG	2 cut(s)	449, 578
SacI	GAGCTC	2 cut(s)	535, 1102
SaqAI	TTAA	2 cut(s)	492, 1617
SchI	GAGTC	7 cut(s)	764, 779, 1065, 1314, 1402, 1551, 2195
SduI	GDGCHC	6 cut(s)	69, 354, 535, 1102, 1580, 2249
SfaNI	GCATC	3 cut(s)	720, 1321, 1713
SfoI	GGCGCC	4 cut(s)	916, 993, 1363, 2070
SfuI	TTCGAA	1 cut(s)	282
SgrAI	CRCCGGYG	1 cut(s)	1832
SinI	GGWCC	7 cut(s)	449, 578, 962, 1051, 1186, 1563, 1807
SmaI	CCCGGG	1 cut(s)	1812
SmiMI	CAYNNNNRTG	1 cut(s)	2255
Sse9I	AATT	7 cut(s)	161, 494, 508, 608, 849, 867, 1789
SspDI	GGCGCC	4 cut(s)	914, 991, 1361, 2068
SspMI	CTAG	2 cut(s)	621, 1055
SstI	GAGCTC	2 cut(s)	535, 1102
StyI	CCWWGG	2 cut(s)	208, 1054
TaaI	ACNGT	8 cut(s)	82, 202, 599, 1009, 1167, 1441, 2020, 2152
TaiI	ACGT	7 cut(s)	192, 279, 384, 837, 1656, 1848, 2009
TaqII	GACCGA	4 cut(s)	437, 1580, 1852, 1890
TasI	AATT	7 cut(s)	161, 494, 508, 608, 849, 867, 1789
TfiI	GAWTC	8 cut(s)	59, 102, 111, 362, 442, 1302, 1449, 1513
Tru1I	TTAA	2 cut(s)	492, 1617
Tru9I	TTAA	2 cut(s)	492, 1617
TscAI	CASTG	3 cut(s)	152, 409, 1170
TseFI	GTSAC	6 cut(s)	979, 1373, 1828, 2020, 2146, 2257
TseI	GCWGC	6 cut(s)	560, 1352, 1556, 1643, 1667, 2230
Tsp45I	GTSAC	6 cut(s)	979, 1373, 1828, 2020, 2146, 2257
TspDTI	ATGAA	6 cut(s)	22, 34, 82, 532, 570, 1752
TspGWI	ACGGA	5 cut(s)	648, 975, 984, 1314, 1625
TspMI	CCCGGG	1 cut(s)	1810
TspRI	CASTG	3 cut(s)	152, 409, 1170
Tth111I	GACNNNGTC	1 cut(s)	1379
VpaK11BI	GGWCC	7 cut(s)	449, 578, 962, 1051, 1186, 1563, 1807
XapI	RAATTY	5 cut(s)	161, 494, 608, 867, 1789
XceI	RCATGY	2 cut(s)	694, 2237
XmaI	CCCGGG	1 cut(s)	1810
XmaJI	CCTAGG	1 cut(s)	1054
XmiI	GTMKAC	3 cut(s)	192, 831, 2049
XmnI	GAANNNNTTC	2 cut(s)	89, 871
XspI	CTAG	2 cut(s)	621, 1055
ZraI	GACGTC	2 cut(s)	382, 835

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.