Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0004305

Physical Location & Seq

Genomic Coordinates Reverse (-)

49687 .. 52402

Gene Structure 2716 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ21870

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2316 bp

ATGGCTACCTTTCTCTCCATTTCCCTCATCTTCATCTTCTTCTTCATATCTCAGATCGAATCAGAGCCCCTCGATCAGACGGTGAAGACCTTCATCTTCAGAATCGACAGATTCTCCAAGCCGTCGATTTTCCAGTCCCATTACCACTGGTACACTTCCGAATTCGCCGACCCACCTCAAATCCTCCACGTATACGACACCGTTTTCCATGGCTTCTCCGCAACTCTCAGCCCCGACCAAGTTGCCTCCCTCAGCCAACACCCTGCCGTCCTCCACGTGTTCGAAGACCGCCGCCGCCAGCTCCACACCACCCGCTCGCCGCAGTTCCTCGGCCTGAGAAACCAGAAGGGGCTCTGGTCCGAATCCGATTACGGCTCTGACGTCATCGTCGGGGTTTTCGACACTGGAGTTTGGCCGGAGCGTCGGAGCTTTTCCGATAGGAATCTCGGTCCGGTTCCGAAGCGGTGGAGAGGGGTCTGTGAGACCGGAGTTAAATTTACAGCTCGGAATTGCAATAAGAAGGTGATCGGAGCTCGGTTCTTCATCAAAGGTCATGAAGCCGCCGCCAATGCTGGCGGTCCGATAACCGCGATCAACGGCTCAGTTGAATTCCGGTCTCCCAGAGATGCCGACGGCCATGGGACTCACACGGCGTCCACCGCCGCCGGGCGGTACGCCTTCGAGGCTAGCATGTCCGGCTACGCTTCCGGCATCGCAAAGGGAGTTGCTCCGAAATCTCGCTTGGCTGTCTACAAGGTCTGCTGGAAGGACTCCGGTTGCTTCGACTCCGATATTCTGGCCGCCTTCGACGCCGCCGTCAACGACGGCGTTGATGTCATTTCAATCTCAATTGGAGGCGGCGATGGAATTTCTTCTCCCTATTATCTCGATCCGATTGCAATCGGATCATTCGGCGCCGTTTCGCATGGAGTGTTTGTTTCGGCCTCCGCCGGGAACGACGGACCTAACGGAATGTCGGTGACGAACCTGGCGCCTTGGCTGACGACTGTCGGAGCGGGAACAATCGATCGGAACTTCCCGGCGGTGGTAGTCCTAGGCGACGGCCGGAGACTCTCTGGCGTTTCTCTATACGCCGGAGCTCCGCTGAAGGGGAAGATGTACCCTCTGGTCTATCCCGGAAAGTCAGGTATGCTCTCTACTTCACTGTGCATGGAGAACTCCCTGGACCCGCGTCAGGTGAGGGGCAAAATCGTAATTTGCGACAGGGGAAACAATCCCAGAGTGGCGAAAGGTATGGTGGTGAAGAAAGCAGGAGGTGTGGGGATGATCCTGGCTAACGGAATCTCCAACGGCGAAGGACTCGTCGGCGATGCTCACCTTCTCCCCACCGCAGCTGTCGGCGCCGACGAAGGTGACGCCGTCAAAGCTTATGTGTCGTCCACCAAGACTCCTTCAGCTACTATCGACTTCCAGGGGACTGTGATCGGAATCAAGCCGGCTCCGGTGGTTGCTTCGTTTTCGGGTAGAGGGCCAAACGGATTGAACCCGGAGATTCTGAAGCCGGATCTGATCGCTCCCGGAGTTAACATCCTCGCTGCTTGGACCGACGCGGTGGGCCCAACCGGGTTGGATGCGGATAACCGGAAAACAGAGTTTAATATCCTCTCGGGAACATCAATGGCTGCCCCTCACGTGAGCGGAGCCGCCGCTCTGCTCAAGTCGGCTCACCCCGATTGGAGTCCGGCGGTGATAAGATCGGCGATGATGACGACTGCTGGCATCACCAATAATCAGAACAAGACCATGACTGATGAAGCTACTGGAAATCCCTCCACTCCTTATGATTTAGGGGCGGGTCATTTGAATTTGGATCGGGCCATGGACCCGGGTCTGGTATATGATATCACCGGTGAGGATTACGTCCGGTTTCTCTGCTCGGTCGGGTACGGGCCGAGAGTAATCCAGGTGATCACTCGGTCGCCGCCGAAATGTCCGGGGAAAAAGACTTCGCCGGGAAATCTGAACTACCCGTCAATCGCCGCGTTGTTTCCGACTTCCTCGGCGGGGCCGTCCAGCAAGACGTTTATTCGGACGGTGACGAATGTGGGCCAGCCCAATGCGGTATACAGGCCCATGATAGAGGCGCCTAAAGGAGTGAAGGTGACGGTGAAGCCGTCGAAGCTGGTGTTCACGGAGGCGGTGAAGAAGCGGAGCTACTTGGTGACGGTGGCGGTGGATAGAAGCAACATCGTGTTGGGTGAGTCGGGGGGTGTGTTCGGGGCGCTATATTGGAGCGATGGGAAGCAGCATGTGGTTCGGAGCCCCATTGTGGTGACCCAAATGGATCCCTTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I9 PA Peptidase_S8

Protein Analysis

771

Amino Acids

81.76

Weight (kDa)

9.04

Isoelectric Point (pI)

36.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I9	PF05922	39 - 102	3.8e-13	Peptidase inhibitor I9
Peptidase_S8	PF00082	125 - 595	2e-46	Subtilase family
PA	PF02225	374 - 460	2.1e-13	PA domain
fn3_6	PF17766	660 - 765	1.8e-27	Fibronectin type-III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004305)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G34980
fragaria_vesca	FvH4_2g01410 FvH4_3g19440
malus_domestica	MD05G1120100.v1.1 MD10G1123500.v1.1
prunus_persica	Prupe.8G164000_v2.0.a1
pyrus_communis	pycom05g11390 pycom10g10580
rosa_chinensis	RchiOBHm_Chr6g0244021 RchiOBHm_Chr6g0252351 RchiOBHm_Chr6g0252361
rosa_laevigata	RLG00000015390 RLG00000029339
rosa_multiflora	Rmu_co8423929.1_g000001 Rmu_sc0001251.1_g000002 Rmu_sc0006415.1_g000012 Rmu_sc0006711.1_g000006 Rmu_sc0007419.1_g000002
rosa_roxburghii	Rroxscaffold_6G00388820 Rroxscaffold_6G00388840
rosa_rugosa	Rorug05G0223500 Rorug05G0506700 Rorug05G0515900
rosa_samantha	Rh1CG388200 Rh2AG211900 Rh3DG088300 Rh5BG286100 Rh5DG294800 Rh6AG021200 Rh6CG013400 Rh6DG014700
rosa_wichuraiana	Rw6G001740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	386, 815
AatII	GACGTC	1 cut(s)	384
AccB1I	GGYRCC	4 cut(s)	914, 991, 1361, 2104
AccBSI	CCGCTC	4 cut(s)	315, 1016, 1659, 1670
AccI	GTMKAC	3 cut(s)	192, 750, 2085
AccII	CGCG	4 cut(s)	590, 1192, 1571, 2003
AclWI	GGATC	7 cut(s)	884, 913, 1282, 1533, 1839, 2300, 2313
AcoI	YGGCCR	4 cut(s)	413, 634, 798, 1063
AcsI	RAATTY	5 cut(s)	161, 494, 608, 867, 1825
AcuI	CTGAAG	4 cut(s)	82, 1127, 1398, 1538
AcvI	CACGTG	2 cut(s)	277, 1654
AcyI	GRCGYC	8 cut(s)	381, 653, 810, 915, 992, 1362, 1377, 2105
AfaI	GTAC	4 cut(s)	152, 674, 1121, 1907
AfiI	CCNNNNNNNGG	9 cut(s)	666, 669, 951, 1045, 1109, 1195, 1852, 2025, 2290
AflIII	ACRYGT	1 cut(s)	276
AgeI	ACCGGT	1 cut(s)	1868
AgsI	TTSAA	4 cut(s)	608, 843, 1504, 1825
AjnI	CCWGG	5 cut(s)	987, 1182, 1290, 1431, 1923
AjuI	GAANNNNNNNTTGG	2 cut(s)	725, 757
Alw21I	GWGCWC	2 cut(s)	535, 1102
Alw26I	GTCTC	3 cut(s)	476, 621, 1063
AlwI	GGATC	7 cut(s)	884, 913, 1282, 1533, 1839, 2300, 2313
Ama87I	CYCGRG	2 cut(s)	1627, 1846
ApaI	GGGCCC	1 cut(s)	1580
ApeKI	GCWGC	4 cut(s)	1352, 1556, 1643, 2266
ApoI	RAATTY	5 cut(s)	161, 494, 608, 867, 1825
ArsI	GACNNNNNNTTYG	2 cut(s)	1308, 1340
AsiGI	ACCGGT	1 cut(s)	1868
Asp700I	GAANNNNTTC	2 cut(s)	89, 871
AspA2I	CCTAGG	1 cut(s)	1054
AspLEI	GCGC	5 cut(s)	917, 994, 1364, 2107, 2245
AsuII	TTCGAA	1 cut(s)	282
AsuNHI	GCTAGC	1 cut(s)	686
AvaI	CYCGRG	2 cut(s)	1627, 1846
AvaII	GGWCC	7 cut(s)	357, 449, 578, 962, 1186, 1563, 1843
AvrII	CCTAGG	1 cut(s)	1054
BaeGI	GKGCMC	1 cut(s)	1580
BamHI	GGATCC	1 cut(s)	2305
BanI	GGYRCC	4 cut(s)	914, 991, 1361, 2104
BanII	GRGCYC	6 cut(s)	69, 354, 535, 1102, 1580, 2285
BarI	GAAGNNNNNNTAC	2 cut(s)	2159, 2191
BbrPI	CACGTG	2 cut(s)	277, 1654
BbsI	GAAGAC	2 cut(s)	92, 291
Bbv12I	GWGCWC	2 cut(s)	535, 1102
BbvCI	CCTCAGC	1 cut(s)	251
BbvI	GCAGC	4 cut(s)	1364, 1543, 1630, 2278
BccI	CCATC	2 cut(s)	857, 2252
BcgI	CGANNNNNNTGC	6 cut(s)	224, 258, 878, 912, 2258, 2292
BciT130I	CCWGG	5 cut(s)	989, 1184, 1292, 1433, 1925
BclI	TGATCA	1 cut(s)	1929
BcoDI	GTCTC	3 cut(s)	476, 621, 1063
BfaI	CTAG	2 cut(s)	687, 1055
BfoI	RGCGCY	5 cut(s)	918, 995, 1365, 2108, 2246
BglI	GCCNNNNNGGC	1 cut(s)	683
BlnI	CCTAGG	1 cut(s)	1054
Bme18I	GGWCC	7 cut(s)	357, 449, 578, 962, 1186, 1563, 1843
BmeT110I	CYCGRG	2 cut(s)	1627, 1846
BmsI	GCATC	5 cut(s)	616, 720, 1321, 1582, 1749
BmtI	GCTAGC	1 cut(s)	690
BoxI	GACNNNNGTC	3 cut(s)	1007, 1191, 1848
BpiI	GAAGAC	2 cut(s)	92, 291
BpmI	CTGGAG	1 cut(s)	426
Bpu10I	CCTNAGC	1 cut(s)	251
Bpu14I	TTCGAA	1 cut(s)	282
BpuEI	CTTGAG	1 cut(s)	1661
Bsa29I	ATCGAT	1 cut(s)	1026
BsaAI	YACGTR	3 cut(s)	190, 277, 1654
BsaBI	GATNNNNATC	3 cut(s)	441, 899, 1448
BsaHI	GRCGYC	8 cut(s)	381, 653, 810, 915, 992, 1362, 1377, 2105
BsaI	GGTCTC	2 cut(s)	476, 621
BsaWI	WCCGGW	8 cut(s)	451, 485, 612, 773, 1462, 1602, 1868, 1884
Bsc4I	CCNNNNNNNGG	9 cut(s)	666, 669, 951, 1045, 1109, 1195, 1852, 2025, 2290
Bse118I	RCCGGY	2 cut(s)	1456, 1868
Bse1I	ACTGG	4 cut(s)	133, 152, 409, 1786
Bse8I	GATNNNNATC	3 cut(s)	441, 899, 1448
BseBI	CCWGG	5 cut(s)	989, 1184, 1292, 1433, 1925
BseCI	ATCGAT	1 cut(s)	1026
BseGI	GGATG	3 cut(s)	1290, 1548, 1597
BseJI	GATNNNNATC	3 cut(s)	441, 899, 1448
BseLI	CCNNNNNNNGG	9 cut(s)	666, 669, 951, 1045, 1109, 1195, 1852, 2025, 2290
BseMII	CTCAG	5 cut(s)	65, 241, 265, 326, 615
BseNI	ACTGG	4 cut(s)	133, 152, 409, 1786
BseSI	GKGCMC	1 cut(s)	1580
BseX3I	CGGCCG	1 cut(s)	1063
BseXI	GCAGC	4 cut(s)	1364, 1543, 1630, 2278
Bsh1236I	CGCG	4 cut(s)	590, 1192, 1571, 2003
Bsh1285I	CGRYCG	4 cut(s)	1030, 1066, 1902, 1940
BshNI	GGYRCC	4 cut(s)	914, 991, 1361, 2104
BshTI	ACCGGT	1 cut(s)	1868
BshVI	ATCGAT	1 cut(s)	1026
BsiEI	CGRYCG	4 cut(s)	1030, 1066, 1902, 1940
BsiHKAI	GWGCWC	2 cut(s)	535, 1102
BsiHKCI	CYCGRG	2 cut(s)	1627, 1846
BslFI	GGGAC	3 cut(s)	121, 655, 1450
BslI	CCNNNNNNNGG	9 cut(s)	666, 669, 951, 1045, 1109, 1195, 1852, 2025, 2290
BsmAI	GTCTC	3 cut(s)	476, 621, 1063
BsmFI	GGGAC	3 cut(s)	121, 655, 1450
Bso31I	GGTCTC	2 cut(s)	476, 621
BsoBI	CYCGRG	2 cut(s)	1627, 1846
Bsp119I	TTCGAA	1 cut(s)	282
Bsp120I	GGGCCC	1 cut(s)	1576
Bsp1286I	GDGCHC	6 cut(s)	69, 354, 535, 1102, 1580, 2285
Bsp19I	CCATGG	3 cut(s)	208, 637, 1839
BspCNI	CTCAG	5 cut(s)	64, 240, 264, 327, 614
BspDI	ATCGAT	1 cut(s)	1026
BspFNI	CGCG	4 cut(s)	590, 1192, 1571, 2003
BspHI	TCATGA	1 cut(s)	553
BspOI	GCTAGC	1 cut(s)	690
BspPI	GGATC	7 cut(s)	884, 913, 1282, 1533, 1839, 2300, 2313
BspT104I	TTCGAA	1 cut(s)	282
BspT107I	GGYRCC	4 cut(s)	914, 991, 1361, 2104
BspTNI	GGTCTC	2 cut(s)	476, 621
BsrBI	CCGCTC	4 cut(s)	315, 1016, 1659, 1670
BsrFI	RCCGGY	2 cut(s)	1456, 1868
BsrI	ACTGG	4 cut(s)	133, 152, 409, 1786
BssAI	RCCGGY	2 cut(s)	1456, 1868
BssNAI	GTATAC	2 cut(s)	193, 2086
BssNI	GRCGYC	8 cut(s)	381, 653, 810, 915, 992, 1362, 1377, 2105
BssT1I	CCWWGG	5 cut(s)	208, 637, 995, 1054, 1839
Bst1107I	GTATAC	2 cut(s)	193, 2086
Bst2UI	CCWGG	5 cut(s)	989, 1184, 1292, 1433, 1925
Bst4CI	ACNGT	8 cut(s)	82, 202, 1009, 1167, 1441, 2056, 2128, 2188
BstACI	GRCGYC	8 cut(s)	381, 653, 810, 915, 992, 1362, 1377, 2105
BstBAI	YACGTR	3 cut(s)	190, 277, 1654
BstBI	TTCGAA	1 cut(s)	282
BstC8I	GCNNGC	7 cut(s)	299, 317, 574, 688, 1458, 1738, 2072
BstDEI	CTNAG	5 cut(s)	51, 227, 251, 335, 601
BstDSI	CCRYGG	3 cut(s)	208, 637, 1839
BstEII	GGTNACC	1 cut(s)	2293
BstF5I	GGATG	3 cut(s)	1290, 1548, 1597
BstFNI	CGCG	4 cut(s)	590, 1192, 1571, 2003
BstH2I	RGCGCY	5 cut(s)	918, 995, 1365, 2108, 2246
BstHHI	GCGC	5 cut(s)	917, 994, 1364, 2107, 2245
BstMAI	GTCTC	3 cut(s)	476, 621, 1063
BstMCI	CGRYCG	4 cut(s)	1030, 1066, 1902, 1940
BstMWI	GCNNNNNNNGC	8 cut(s)	569, 659, 683, 696, 809, 1361, 1385, 2140
BstNI	CCWGG	5 cut(s)	989, 1184, 1292, 1433, 1925
BstNSI	RCATGY	2 cut(s)	694, 2273
BstPAI	GACNNNNGTC	3 cut(s)	1007, 1191, 1848
BstPI	GGTNACC	1 cut(s)	2293
BstSLI	GKGCMC	1 cut(s)	1580
BstUI	CGCG	4 cut(s)	590, 1192, 1571, 2003
BstV1I	GCAGC	4 cut(s)	1364, 1543, 1630, 2278
BstV2I	GAAGAC	2 cut(s)	92, 291
BstX2I	RGATCY	2 cut(s)	1525, 2305
BstYI	RGATCY	2 cut(s)	1525, 2305
BstZ17I	GTATAC	2 cut(s)	193, 2086
BstZI	CGGCCG	1 cut(s)	1063
Bsu15I	ATCGAT	1 cut(s)	1026
BsuTUI	ATCGAT	1 cut(s)	1026
BtgI	CCRYGG	3 cut(s)	208, 637, 1839
BtgZI	GCGATG	5 cut(s)	697, 876, 1344, 1736, 2271
BtsCI	GGATG	3 cut(s)	1290, 1548, 1597
BtsIMutI	CAGTG	3 cut(s)	145, 402, 1163
Cac8I	GCNNGC	7 cut(s)	299, 317, 574, 688, 1458, 1738, 2072
CciI	TCATGA	1 cut(s)	553
CfoI	GCGC	5 cut(s)	917, 994, 1364, 2107, 2245
Cfr10I	RCCGGY	2 cut(s)	1456, 1868
Cfr9I	CCCGGG	1 cut(s)	1846
ClaI	ATCGAT	1 cut(s)	1026
CpoI	CGGWCCG	2 cut(s)	449, 578
CseI	GACGC	6 cut(s)	410, 642, 818, 1181, 1385, 1577
Csp6I	GTAC	4 cut(s)	151, 673, 1120, 1906
CspAI	ACCGGT	1 cut(s)	1868
CspI	CGGWCCG	2 cut(s)	449, 578
CviQI	GTAC	4 cut(s)	151, 673, 1120, 1906
DdeI	CTNAG	5 cut(s)	51, 227, 251, 335, 601
DinI	GGCGCC	4 cut(s)	916, 993, 1363, 2106
DrdI	GACNNNNNNGTC	2 cut(s)	386, 815
DseDI	GACNNNNNNGTC	2 cut(s)	386, 815
EaeI	YGGCCR	4 cut(s)	413, 634, 798, 1063
EagI	CGGCCG	1 cut(s)	1063
EciI	GGCGGA	1 cut(s)	937
Ecl136II	GAGCTC	2 cut(s)	533, 1100
EclXI	CGGCCG	1 cut(s)	1063
Eco130I	CCWWGG	5 cut(s)	208, 637, 995, 1054, 1839
Eco24I	GRGCYC	6 cut(s)	69, 354, 535, 1102, 1580, 2285
Eco31I	GGTCTC	2 cut(s)	476, 621
Eco32I	GATATC	1 cut(s)	1864
Eco47I	GGWCC	7 cut(s)	357, 449, 578, 962, 1186, 1563, 1843
Eco52I	CGGCCG	1 cut(s)	1063
Eco53kI	GAGCTC	2 cut(s)	533, 1100
Eco57I	CTGAAG	4 cut(s)	82, 1127, 1398, 1538
Eco72I	CACGTG	2 cut(s)	277, 1654
Eco88I	CYCGRG	2 cut(s)	1627, 1846
Eco91I	GGTNACC	1 cut(s)	2293
EcoICRI	GAGCTC	2 cut(s)	533, 1100
EcoO65I	GGTNACC	1 cut(s)	2293
EcoRI	GAATTC	2 cut(s)	161, 608
EcoRII	CCWGG	5 cut(s)	987, 1182, 1290, 1431, 1923
EcoRV	GATATC	1 cut(s)	1864
EcoT14I	CCWWGG	5 cut(s)	208, 637, 995, 1054, 1839
EcoT38I	GRGCYC	6 cut(s)	69, 354, 535, 1102, 1580, 2285
EgeI	GGCGCC	4 cut(s)	916, 993, 1363, 2106
EheI	GGCGCC	4 cut(s)	916, 993, 1363, 2106
ErhI	CCWWGG	5 cut(s)	208, 637, 995, 1054, 1839
FaqI	GGGAC	3 cut(s)	121, 655, 1450
FauI	CCCGC	5 cut(s)	320, 1009, 1197, 1807, 2017
FbaI	TGATCA	1 cut(s)	1929
FblI	GTMKAC	3 cut(s)	192, 750, 2085
FokI	GGATG	3 cut(s)	1297, 1535, 1604
FriOI	GRGCYC	6 cut(s)	69, 354, 535, 1102, 1580, 2285
FspBI	CTAG	2 cut(s)	687, 1055
GlaI	GCGC	5 cut(s)	916, 993, 1363, 2106, 2244
GsuI	CTGGAG	1 cut(s)	426
HaeII	RGCGCY	5 cut(s)	918, 995, 1365, 2108, 2246
HgaI	GACGC	6 cut(s)	410, 642, 818, 1181, 1385, 1577
HhaI	GCGC	5 cut(s)	917, 994, 1364, 2107, 2245
Hin1I	GRCGYC	8 cut(s)	381, 653, 810, 915, 992, 1362, 1377, 2105
Hin6I	GCGC	5 cut(s)	915, 992, 1362, 2105, 2243
HinP1I	GCGC	5 cut(s)	915, 992, 1362, 2105, 2243
HincII	GTYRAC	2 cut(s)	820, 1546
HindII	GTYRAC	2 cut(s)	820, 1546
HindIII	AAGCTT	1 cut(s)	1386
HpaI	GTTAAC	1 cut(s)	1546
Hpy166II	GTNNAC	9 cut(s)	153, 193, 657, 751, 820, 1401, 1546, 2086, 2151
Hpy188III	TCNNGA	3 cut(s)	554, 887, 1629
Hpy8I	GTNNAC	9 cut(s)	153, 193, 657, 751, 820, 1401, 1546, 2086, 2151
HpyCH4III	ACNGT	8 cut(s)	82, 202, 1009, 1167, 1441, 2056, 2128, 2188
HpyCH4IV	ACGT	6 cut(s)	189, 276, 381, 1653, 1881, 2042
HpyCH4V	TGCA	3 cut(s)	513, 899, 1170
HpyF10VI	GCNNNNNNNGC	8 cut(s)	569, 659, 683, 696, 809, 1361, 1385, 2140
HpyF3I	CTNAG	5 cut(s)	51, 227, 251, 335, 601
HpySE526I	ACGT	6 cut(s)	189, 276, 381, 1653, 1881, 2042
Hsp92I	GRCGYC	8 cut(s)	381, 653, 810, 915, 992, 1362, 1377, 2105
HspAI	GCGC	5 cut(s)	915, 992, 1362, 2105, 2243
KasI	GGCGCC	4 cut(s)	914, 991, 1361, 2104
KroI	GCCGGC	1 cut(s)	1456
KroNI	GCCGGC	1 cut(s)	1458
Ksp22I	TGATCA	1 cut(s)	1929
KspAI	GTTAAC	1 cut(s)	1546
Lsp1109I	GCAGC	4 cut(s)	1364, 1543, 1630, 2278
LweI	GCATC	5 cut(s)	616, 720, 1321, 1582, 1749
MaeI	CTAG	2 cut(s)	687, 1055
MaeII	ACGT	6 cut(s)	189, 276, 381, 1653, 1881, 2042
MaeIII	GTNAC	6 cut(s)	979, 1373, 2056, 2122, 2182, 2293
MbiI	CCGCTC	4 cut(s)	315, 1016, 1659, 1670
MfeI	CAATTG	1 cut(s)	849
MflI	RGATCY	2 cut(s)	1525, 2305
MhlI	GDGCHC	6 cut(s)	69, 354, 535, 1102, 1580, 2285
MluCI	AATT	8 cut(s)	161, 494, 508, 608, 849, 867, 1215, 1825
Mly113I	GGCGCC	4 cut(s)	915, 992, 1362, 2105
MlyI	GAGTC	8 cut(s)	637, 764, 779, 1065, 1314, 1402, 1708, 2231
MmeI	TCCRAC	5 cut(s)	404, 991, 1332, 1569, 2036
MroNI	GCCGGC	1 cut(s)	1456
MroXI	GAANNNNTTC	2 cut(s)	89, 871
MseI	TTAA	3 cut(s)	492, 1545, 1617
MslI	CAYNNNNRTG	1 cut(s)	2291
MspA1I	CMGCKG	2 cut(s)	1105, 1355
MunI	CAATTG	1 cut(s)	849
MvaI	CCWGG	5 cut(s)	989, 1184, 1292, 1433, 1925
MvnI	CGCG	4 cut(s)	590, 1192, 1571, 2003
MwoI	GCNNNNNNNGC	8 cut(s)	569, 659, 683, 696, 809, 1361, 1385, 2140
NaeI	GCCGGC	1 cut(s)	1458
NarI	GGCGCC	4 cut(s)	915, 992, 1362, 2105
NcoI	CCATGG	3 cut(s)	208, 637, 1839
NgoMIV	GCCGGC	1 cut(s)	1456
NheI	GCTAGC	1 cut(s)	686
NmeAIII	GCCGAG	3 cut(s)	309, 1938, 2000
NmuCI	GTSAC	6 cut(s)	979, 1373, 2056, 2122, 2182, 2293
NspI	RCATGY	2 cut(s)	694, 2273
NspV	TTCGAA	1 cut(s)	282
PagI	TCATGA	1 cut(s)	553
PcsI	WCGNNNNNNNCGW	7 cut(s)	378, 396, 813, 1218, 1365, 2027, 2132
PdiI	GCCGGC	1 cut(s)	1458
PdmI	GAANNNNTTC	2 cut(s)	89, 871
PfeI	GAWTC	8 cut(s)	59, 102, 111, 362, 442, 1302, 1449, 1513
PflFI	GACNNNGTC	1 cut(s)	1379
PfoI	TCCNGGA	2 cut(s)	1135, 1537
PinAI	ACCGGT	1 cut(s)	1868
Ple19I	CGATCG	1 cut(s)	1030
PleI	GAGTC	8 cut(s)	637, 764, 779, 1065, 1314, 1402, 1707, 2230
PluTI	GGCGCC	4 cut(s)	918, 995, 1365, 2108
PmaCI	CACGTG	2 cut(s)	277, 1654
PmlI	CACGTG	2 cut(s)	277, 1654
PpsI	GAGTC	8 cut(s)	637, 764, 779, 1065, 1314, 1402, 1707, 2230
Ppu21I	YACGTR	3 cut(s)	190, 277, 1654
PshAI	GACNNNNGTC	3 cut(s)	1007, 1191, 1848
Psp124BI	GAGCTC	2 cut(s)	535, 1102
Psp6I	CCWGG	5 cut(s)	987, 1182, 1290, 1431, 1923
PspCI	CACGTG	2 cut(s)	277, 1654
PspEI	GGTNACC	1 cut(s)	2293
PspGI	CCWGG	5 cut(s)	987, 1182, 1290, 1431, 1923
PspOMI	GGGCCC	1 cut(s)	1576
PsuI	RGATCY	2 cut(s)	1525, 2305
PsyI	GACNNNGTC	1 cut(s)	1379
PvuI	CGATCG	1 cut(s)	1030
PvuII	CAGCTG	1 cut(s)	1355
RsaI	GTAC	4 cut(s)	152, 674, 1121, 1907
RsaNI	GTAC	4 cut(s)	151, 673, 1120, 1906
RseI	CAYNNNNRTG	1 cut(s)	2291
Rsr2I	CGGWCCG	2 cut(s)	449, 578
RsrII	CGGWCCG	2 cut(s)	449, 578
SacI	GAGCTC	2 cut(s)	535, 1102
SaqAI	TTAA	3 cut(s)	492, 1545, 1617
SchI	GAGTC	8 cut(s)	637, 764, 779, 1065, 1314, 1402, 1708, 2231
SduI	GDGCHC	6 cut(s)	69, 354, 535, 1102, 1580, 2285
SfaNI	GCATC	5 cut(s)	616, 720, 1321, 1582, 1749
SfoI	GGCGCC	4 cut(s)	916, 993, 1363, 2106
SfuI	TTCGAA	1 cut(s)	282
SgrAI	CRCCGGYG	1 cut(s)	1868
SinI	GGWCC	7 cut(s)	357, 449, 578, 962, 1186, 1563, 1843
SmaI	CCCGGG	1 cut(s)	1848
SmiMI	CAYNNNNRTG	1 cut(s)	2291
SmlI	CTYRAG	1 cut(s)	1676
SmoI	CTYRAG	1 cut(s)	1676
Sse9I	AATT	8 cut(s)	161, 494, 508, 608, 849, 867, 1215, 1825
SspDI	GGCGCC	4 cut(s)	914, 991, 1361, 2104
SspMI	CTAG	2 cut(s)	687, 1055
SstI	GAGCTC	2 cut(s)	535, 1102
StyI	CCWWGG	5 cut(s)	208, 637, 995, 1054, 1839
TaaI	ACNGT	8 cut(s)	82, 202, 1009, 1167, 1441, 2056, 2128, 2188
TaiI	ACGT	6 cut(s)	192, 279, 384, 1656, 1884, 2045
TaqII	GACCGA	4 cut(s)	437, 1580, 1888, 1926
TasI	AATT	8 cut(s)	161, 494, 508, 608, 849, 867, 1215, 1825
TfiI	GAWTC	8 cut(s)	59, 102, 111, 362, 442, 1302, 1449, 1513
Tru1I	TTAA	3 cut(s)	492, 1545, 1617
Tru9I	TTAA	3 cut(s)	492, 1545, 1617
TscAI	CASTG	3 cut(s)	152, 409, 1170
TseFI	GTSAC	6 cut(s)	979, 1373, 2056, 2122, 2182, 2293
TseI	GCWGC	4 cut(s)	1352, 1556, 1643, 2266
Tsp45I	GTSAC	6 cut(s)	979, 1373, 2056, 2122, 2182, 2293
TspDTI	ATGAA	6 cut(s)	22, 34, 82, 532, 570, 1788
TspGWI	ACGGA	5 cut(s)	975, 984, 1314, 1512, 2168
TspMI	CCCGGG	1 cut(s)	1846
TspRI	CASTG	3 cut(s)	152, 409, 1170
Tth111I	GACNNNGTC	1 cut(s)	1379
VpaK11BI	GGWCC	7 cut(s)	357, 449, 578, 962, 1186, 1563, 1843
XapI	RAATTY	5 cut(s)	161, 494, 608, 867, 1825
XceI	RCATGY	2 cut(s)	694, 2273
XmaI	CCCGGG	1 cut(s)	1846
XmaJI	CCTAGG	1 cut(s)	1054
XmiI	GTMKAC	3 cut(s)	192, 750, 2085
XmnI	GAANNNNTTC	2 cut(s)	89, 871
XspI	CTAG	2 cut(s)	687, 1055
ZraI	GACGTC	1 cut(s)	382

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.