FvH4_3g22471

DTW

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0012344
Physical Location & Seq
Forward (+)
15501847 .. 15504157
2311 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g22471.t1

Sequence Viewer

Length: 1485 bp
ATGACATCTAACAGTACTAAAAGAAAAAGGAAACTTTATAAATGTGTTCTCACAAAGATGCATGGAAACAATAAAAACAGAGGAGTCTTTACAATGTTGGTTCGGTTCTCACAGACCCGAAACCGACATTTCCCCCCGATCCTCCATAAACCCTACTCACTTCTTCTTCCCCAACCCCTGAAAATGCTGACTCCAACTCAGGCCATCTCGAAAAGACCCATTTGCCCTTCCTGCTCCAAACCCGTCCGGCTCTGCCTCTGCAGTCGGATCCACAATCCGGGGCTCCACAACTCTGTAAGTGTCACCATTCTTCAACACAGCTTTGAGAAGAAGCACCCTCTTAATTCAGCTAGGATTGCTAGACTTGGGCTTAAGAATGTGAAGGTGGTTACTGTGTTTGATGTTCATTTTGATGCCCGGTTCGTTATCCGGTTGCTCGACCCGGATTTGGACCCGAATGGTTCAAAGGGTGCATCTTTTGATGGAGGTGTGGGTTCTGGGTTTTGTGTTGAGTCAGAAATTGTGGGTTTTGATAGTTTAGAGAAACAGAGGAGTTGTGGTAGTGTGATTGATTGTAATGAGGAATGTAGTCTTGGGAATGATATAGGTAATGTGAGGAGTGCTCAAAATGAATTAGGAGGTGTAGAATATGTAGAAAATTGTAGCGTATCGAGCGGTGATGCATTACTTGTAGAGCAAGAACATGAGTTCACTAGTGATGCATGCACTGATGTAAAGGGTGTGTTTAATCTGGAGACTTGTGATAGGCATTTGGAAGGACAATCTGGTGTGGATGAGGATTCAACTGCAACGACATTGGCTTCACTATGTGATGCCGATACGGATTTTGAGTCAGTTGAAGGTGTTACAGTTGGTAATGATGGACCTGTTATAATGGCTACAATTGGCAAGAATGGCATTAGCTCTCTTACTCATACTTGGATGCCACAAACCCATCATAGGAATTCACAGTTTGATGCAATTTCAGAGATTACAGAAGCGCGTGAGGCTCTGGCAAAGGGATTTGTTGTACAAAAGTTGCAAAGGCAGCCATCGAAAGGAAATGTGGAATTGGATGAAACAGTAGAATTTGAAGTAGAAGTTCCTCCGGGATCTGTTCTCTTGTTTCCATCTGAAGAAGCAGTTAGTGTTAGTGACCTTGAAGCGATGGATATTAAGGTCAAGAATTTGATTGTCTTGGATGGAACATGGTCTAAGGCAAAGAGAATGTATGTGGAAAACCCCTGGTTGAAGCTATTGCCACATTTGAGGTTGGATTTGGACAAGCTGAGTTTGTACAATGAAGTGAGAGTCCAACCCAAGCCGGGATTTCTGTCCACTATTGAGAGCATTGTATACGCACTAAAGGCGGTAGGGGACAGTCCGGAAGGCCTGGACAATCTCTTGGATGTTTTCGAGTCCATGGTTGGAGACCAGAGACGATGCAAAGAAGAAAGAATAAGCAATGTCTTGCCGCCTGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DTW

Protein Analysis

495

Amino Acids

54.56

Weight (kDa)

5.76

Isoelectric Point (pI)

44.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DTW PF03942 72 - 132 1e-09 DTW domain
DTW PF03942 315 - 479 1.2e-27 DTW domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012344)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G54880
fragaria_vesca FvH4_3g22471
malus_domestica MD03G1204100.v1.1 MD11G1220900.v1.1
prunus_persica Prupe.4G211300_v2.0.a1
pyrus_communis pycom03g15470 pycom11g19490
rosa_chinensis RchiOBHm_Chr5g0039141
rosa_laevigata RLG00000033897
rosa_multiflora Rmu_sc0000533.1_g000008 Rmu_ssc0000410.1_g000018 Rmu_ssc0000410.1_g000044
rosa_roxburghii Rroxscaffold_1G00040360
rosa_rugosa Rorug05G0177000
rosa_samantha Rh5BG268300 Rh5CG301100 Rh5DG276100
rosa_wichuraiana Rw0G001970 Rw5G024630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 39, 893
AccBSI CCGCTC 1 cut(s) 675
AccI GTMKAC 1 cut(s) 1356
AccII CGCG 1 cut(s) 1003
AccIII TCCGGA 1 cut(s) 1384
AciI CCGC 3 cut(s) 675, 1370, 1475
AclWI GGATC 4 cut(s) 133, 262, 275, 1120
AcsI RAATTY 3 cut(s) 964, 1088, 1186
AcuI CTGAAG 1 cut(s) 1155
AdeI CACNNNGTG 1 cut(s) 830
AfaI GTAC 3 cut(s) 16, 1032, 1298
AfiI CCNNNNNNNGG 5 cut(s) 277, 448, 960, 1058, 1325
AflII CTTAAG 1 cut(s) 371
AgsI TTSAA 7 cut(s) 314, 465, 804, 860, 1094, 1163, 1252
AhlI ACTAGT 1 cut(s) 713
AjnI CCWGG 2 cut(s) 1244, 1392
AjuI GAANNNNNNNTTGG 2 cut(s) 576, 608
AluBI AGCT 5 cut(s) 321, 350, 924, 1255, 1288
AluI AGCT 5 cut(s) 321, 350, 924, 1255, 1288
Alw21I GWGCWC 1 cut(s) 625
Alw26I GTCTC 3 cut(s) 749, 1425, 1432
AlwI GGATC 4 cut(s) 133, 262, 275, 1120
Aor13HI TCCGGA 1 cut(s) 1384
AoxI GGCC 2 cut(s) 201, 1390
ApeKI GCWGC 1 cut(s) 1048
ApoI RAATTY 3 cut(s) 964, 1088, 1186
AspLEI GCGC 1 cut(s) 1003
AspS9I GGNCC 2 cut(s) 451, 884
AsuC2I CCSGG 5 cut(s) 279, 418, 443, 1110, 1326
AsuHPI GGTGA 2 cut(s) 295, 689
AvaII GGWCC 2 cut(s) 451, 884
BamHI GGATCC 1 cut(s) 267
BanII GRGCYC 1 cut(s) 285
Bbv12I GWGCWC 1 cut(s) 625
BbvI GCAGC 1 cut(s) 1060
BccI CCATC 8 cut(s) 212, 476, 875, 963, 1060, 1138, 1162, 1196
BciT130I CCWGG 2 cut(s) 1246, 1394
BcnI CCSGG 5 cut(s) 279, 418, 443, 1110, 1326
BcoDI GTCTC 3 cut(s) 749, 1425, 1432
BcuI ACTAGT 1 cut(s) 713
BfaI CTAG 3 cut(s) 351, 360, 714
BfmI CTRYAG 1 cut(s) 259
BfrI CTTAAG 1 cut(s) 371
BisI GCNGC 2 cut(s) 1049, 1475
BlsI GCNGC 2 cut(s) 1050, 1476
BmcAI AGTACT 1 cut(s) 16
Bme1390I CCNGG 7 cut(s) 279, 418, 443, 1110, 1246, 1326, 1394
Bme18I GGWCC 2 cut(s) 451, 884
BmgT120I GGNCC 2 cut(s) 451, 884
BmiI GGNNCC 3 cut(s) 269, 284, 453
BmrFI CCNGG 7 cut(s) 279, 418, 443, 1110, 1246, 1326, 1394
BmsI GCATC 9 cut(s) 48, 403, 482, 670, 709, 823, 933, 967, 1433
BplI GAGNNNNNCTC 2 cut(s) 607, 639
BpmI CTGGAG 1 cut(s) 773
BpuMI CCSGG 5 cut(s) 279, 418, 443, 1110, 1326
BsaI GGTCTC 1 cut(s) 1425
BsaJI CCNNGG 3 cut(s) 278, 1244, 1422
BsaWI WCCGGW 2 cut(s) 429, 1384
BsaXI ACNNNNNCTCC 2 cut(s) 544, 574
Bsc4I CCNNNNNNNGG 5 cut(s) 277, 448, 960, 1058, 1325
Bse3DI GCAATG 1 cut(s) 1471
BseAI TCCGGA 1 cut(s) 1384
BseBI CCWGG 2 cut(s) 1246, 1394
BseDI CCNNGG 3 cut(s) 278, 1244, 1422
BseGI GGATG 5 cut(s) 799, 948, 1081, 1207, 1414
BseLI CCNNNNNNNGG 5 cut(s) 277, 448, 960, 1058, 1325
BseMI GCAATG 1 cut(s) 1471
BseMII CTCAG 3 cut(s) 212, 1280, 1470
BseRI GAGGAG 3 cut(s) 96, 565, 631
BseXI GCAGC 1 cut(s) 1060
Bsh1236I CGCG 1 cut(s) 1003
BshFI GGCC 2 cut(s) 203, 1392
BsiHKAI GWGCWC 1 cut(s) 625
BsiSI CCGG 8 cut(s) 247, 278, 418, 430, 443, 1109, 1325, 1385
BslFI GGGAC 1 cut(s) 1391
BslI CCNNNNNNNGG 5 cut(s) 277, 448, 960, 1058, 1325
BsmAI GTCTC 3 cut(s) 749, 1425, 1432
BsmBI CGTCTC 1 cut(s) 1432
BsmFI GGGAC 1 cut(s) 1391
BsnI GGCC 2 cut(s) 203, 1392
Bso31I GGTCTC 1 cut(s) 1425
Bsp1286I GDGCHC 2 cut(s) 285, 625
Bsp13I TCCGGA 1 cut(s) 1384
Bsp1407I TGTACA 2 cut(s) 1030, 1296
Bsp143I GATC 3 cut(s) 138, 267, 1112
Bsp19I CCATGG 1 cut(s) 1422
BspACI CCGC 3 cut(s) 675, 1370, 1475
BspANI GGCC 2 cut(s) 203, 1392
BspCNI CTCAG 3 cut(s) 211, 1281, 1471
BspEI TCCGGA 1 cut(s) 1384
BspFNI CGCG 1 cut(s) 1003
BspLI GGNNCC 3 cut(s) 269, 284, 453
BspMAI CTGCAG 1 cut(s) 263
BspPI GGATC 4 cut(s) 133, 262, 275, 1120
BspTI CTTAAG 1 cut(s) 371
BspTNI GGTCTC 1 cut(s) 1425
BsrBI CCGCTC 1 cut(s) 675
BsrDI GCAATG 1 cut(s) 1471
BsrGI TGTACA 2 cut(s) 1030, 1296
BssECI CCNNGG 3 cut(s) 278, 1244, 1422
BssMI GATC 3 cut(s) 138, 267, 1112
BssNAI GTATAC 1 cut(s) 1357
BssT1I CCWWGG 1 cut(s) 1422
Bst1107I GTATAC 1 cut(s) 1357
Bst2UI CCWGG 2 cut(s) 1246, 1394
Bst4CI ACNGT 6 cut(s) 14, 394, 871, 972, 1084, 1382
BstAFI CTTAAG 1 cut(s) 371
BstAUI TGTACA 2 cut(s) 1030, 1296
BstC8I GCNNGC 1 cut(s) 724
BstDEI CTNAG 4 cut(s) 198, 1215, 1289, 1479
BstDSI CCRYGG 1 cut(s) 1422
BstF5I GGATG 5 cut(s) 799, 948, 1081, 1207, 1414
BstFNI CGCG 1 cut(s) 1003
BstHHI GCGC 1 cut(s) 1003
BstKTI GATC 3 cut(s) 141, 270, 1115
BstMAI GTCTC 3 cut(s) 749, 1425, 1432
BstMBI GATC 3 cut(s) 138, 267, 1112
BstMWI GCNNNNNNNGC 7 cut(s) 231, 356, 672, 915, 1007, 1048, 1367
BstNI CCWGG 2 cut(s) 1246, 1394
BstNSI RCATGY 1 cut(s) 726
BstSCI CCNGG 7 cut(s) 277, 416, 441, 1108, 1244, 1324, 1392
BstSFI CTRYAG 1 cut(s) 259
BstUI CGCG 1 cut(s) 1003
BstV1I GCAGC 1 cut(s) 1060
BstX2I RGATCY 2 cut(s) 267, 1112
BstYI RGATCY 2 cut(s) 267, 1112
BstZ17I GTATAC 1 cut(s) 1357
BsuRI GGCC 2 cut(s) 203, 1392
BtgI CCRYGG 1 cut(s) 1422
BtgZI GCGATG 1 cut(s) 1181
BtsCI GGATG 5 cut(s) 799, 948, 1081, 1207, 1414
BtsIMutI CAGTG 1 cut(s) 726
Cac8I GCNNGC 1 cut(s) 724
CfoI GCGC 1 cut(s) 1003
Cfr13I GGNCC 2 cut(s) 451, 884
Csp6I GTAC 3 cut(s) 15, 1031, 1297
CviAII CATG 5 cut(s) 62, 704, 723, 1209, 1423
CviQI GTAC 3 cut(s) 15, 1031, 1297
DdeI CTNAG 4 cut(s) 198, 1215, 1289, 1479
DpnI GATC 3 cut(s) 140, 269, 1114
DpnII GATC 3 cut(s) 138, 267, 1112
DraIII CACNNNGTG 1 cut(s) 830
Eco130I CCWWGG 1 cut(s) 1422
Eco147I AGGCCT 1 cut(s) 1392
Eco24I GRGCYC 1 cut(s) 285
Eco31I GGTCTC 1 cut(s) 1425
Eco47I GGWCC 2 cut(s) 451, 884
Eco57I CTGAAG 1 cut(s) 1155
EcoRI GAATTC 1 cut(s) 964
EcoRII CCWGG 2 cut(s) 1244, 1392
EcoT14I CCWWGG 1 cut(s) 1422
EcoT22I ATGCAT 3 cut(s) 63, 685, 724
EcoT38I GRGCYC 1 cut(s) 285
ErhI CCWWGG 1 cut(s) 1422
Esp3I CGTCTC 1 cut(s) 1432
FaeI CATG 5 cut(s) 65, 707, 726, 1212, 1426
FaqI GGGAC 1 cut(s) 1391
FatI CATG 5 cut(s) 61, 703, 722, 1208, 1422
FblI GTMKAC 1 cut(s) 1356
Fnu4HI GCNGC 2 cut(s) 1049, 1475
FokI GGATG 5 cut(s) 806, 955, 1088, 1214, 1421
FriOI GRGCYC 1 cut(s) 285
Fsp4HI GCNGC 2 cut(s) 1049, 1475
FspBI CTAG 3 cut(s) 351, 360, 714
GlaI GCGC 1 cut(s) 1002
GluI GCNGC 2 cut(s) 1049, 1475
GsuI CTGGAG 1 cut(s) 773
HaeIII GGCC 2 cut(s) 203, 1392
HapII CCGG 8 cut(s) 247, 278, 418, 430, 443, 1109, 1325, 1385
HhaI GCGC 1 cut(s) 1003
Hin1II CATG 5 cut(s) 65, 707, 726, 1212, 1426
Hin6I GCGC 1 cut(s) 1001
HinP1I GCGC 1 cut(s) 1001
HinfI GANTC 7 cut(s) 84, 190, 512, 800, 851, 1311, 1418
HpaII CCGG 8 cut(s) 247, 278, 418, 430, 443, 1109, 1325, 1385
HphI GGTGA 2 cut(s) 295, 689
Hpy166II GTNNAC 3 cut(s) 711, 1338, 1357
Hpy188I TCNGA 4 cut(s) 267, 517, 988, 1135
Hpy188III TCNNGA 4 cut(s) 208, 752, 1183, 1385
Hpy8I GTNNAC 3 cut(s) 711, 1338, 1357
HpyAV CCTTC 5 cut(s) 237, 376, 770, 854, 1382
HpyCH4III ACNGT 6 cut(s) 14, 394, 871, 972, 1084, 1382
HpyF10VI GCNNNNNNNGC 7 cut(s) 231, 356, 672, 915, 1007, 1048, 1367
HpyF3I CTNAG 4 cut(s) 198, 1215, 1289, 1479
Hsp92II CATG 5 cut(s) 65, 707, 726, 1212, 1426
HspAI GCGC 1 cut(s) 1001
Kpn2I TCCGGA 1 cut(s) 1384
Kzo9I GATC 3 cut(s) 138, 267, 1112
LmnI GCTCC 2 cut(s) 239, 288
Lsp1109I GCAGC 1 cut(s) 1060
LweI GCATC 9 cut(s) 48, 403, 482, 670, 709, 823, 933, 967, 1433
MaeI CTAG 3 cut(s) 351, 360, 714
MaeIII GTNAC 4 cut(s) 301, 388, 865, 1154
MalI GATC 3 cut(s) 140, 269, 1114
MbiI CCGCTC 1 cut(s) 675
MboI GATC 3 cut(s) 138, 267, 1112
MboII GAAGA 6 cut(s) 155, 158, 302, 340, 1148, 1463
MfeI CAATTG 1 cut(s) 903
MflI RGATCY 2 cut(s) 267, 1112
MhlI GDGCHC 2 cut(s) 285, 625
MlyI GAGTC 6 cut(s) 93, 184, 521, 860, 1320, 1427
MmeI TCCRAC 5 cut(s) 218, 245, 1254, 1339, 1408
Mph1103I ATGCAT 3 cut(s) 63, 685, 724
MroI TCCGGA 1 cut(s) 1384
MseI TTAA 4 cut(s) 342, 372, 747, 1176
MslI CAYNNNNRTG 2 cut(s) 56, 411
MspCI CTTAAG 1 cut(s) 371
MspI CCGG 8 cut(s) 247, 278, 418, 430, 443, 1109, 1325, 1385
MspR9I CCNGG 7 cut(s) 279, 418, 443, 1110, 1246, 1326, 1394
MunI CAATTG 1 cut(s) 903
MvaI CCWGG 2 cut(s) 1246, 1394
MvnI CGCG 1 cut(s) 1003
MwoI GCNNNNNNNGC 7 cut(s) 231, 356, 672, 915, 1007, 1048, 1367
NciI CCSGG 5 cut(s) 279, 418, 443, 1110, 1326
NcoI CCATGG 1 cut(s) 1422
NdeII GATC 3 cut(s) 138, 267, 1112
NlaIII CATG 5 cut(s) 65, 707, 726, 1212, 1426
NlaIV GGNNCC 3 cut(s) 269, 284, 453
NmuCI GTSAC 2 cut(s) 301, 1154
NsiI ATGCAT 3 cut(s) 63, 685, 724
NspI RCATGY 1 cut(s) 726
PaeI GCATGC 1 cut(s) 726
PceI AGGCCT 1 cut(s) 1392
PfeI GAWTC 1 cut(s) 800
PfoI TCCNGGA 1 cut(s) 1108
PkrI GCNGC 2 cut(s) 1050, 1476
PleI GAGTC 6 cut(s) 92, 184, 520, 859, 1319, 1426
PpsI GAGTC 6 cut(s) 92, 184, 520, 859, 1319, 1426
PsiI TTATAA 2 cut(s) 39, 893
Psp6I CCWGG 2 cut(s) 1244, 1392
PspGI CCWGG 2 cut(s) 1244, 1392
PspN4I GGNNCC 3 cut(s) 269, 284, 453
PspPI GGNCC 2 cut(s) 451, 884
PstI CTGCAG 1 cut(s) 263
PsuI RGATCY 2 cut(s) 267, 1112
RsaI GTAC 3 cut(s) 16, 1032, 1298
RsaNI GTAC 3 cut(s) 15, 1031, 1297
RseI CAYNNNNRTG 2 cut(s) 56, 411
SaqAI TTAA 4 cut(s) 342, 372, 747, 1176
SatI GCNGC 2 cut(s) 1049, 1475
Sau3AI GATC 3 cut(s) 138, 267, 1112
Sau96I GGNCC 2 cut(s) 451, 884
ScaI AGTACT 1 cut(s) 16
SchI GAGTC 6 cut(s) 93, 184, 521, 860, 1320, 1427
ScrFI CCNGG 7 cut(s) 279, 418, 443, 1110, 1246, 1326, 1394
SduI GDGCHC 2 cut(s) 285, 625
SfaNI GCATC 9 cut(s) 48, 403, 482, 670, 709, 823, 933, 967, 1433
SfcI CTRYAG 1 cut(s) 259
SinI GGWCC 2 cut(s) 451, 884
SmiMI CAYNNNNRTG 2 cut(s) 56, 411
SmlI CTYRAG 1 cut(s) 371
SmoI CTYRAG 1 cut(s) 371
SpeI ACTAGT 1 cut(s) 713
SphI GCATGC 1 cut(s) 726
SseBI AGGCCT 1 cut(s) 1392
SsiI CCGC 3 cut(s) 675, 1370, 1475
SspMI CTAG 3 cut(s) 351, 360, 714
StuI AGGCCT 1 cut(s) 1392
StyD4I CCNGG 7 cut(s) 277, 416, 441, 1108, 1244, 1324, 1392
StyI CCWWGG 1 cut(s) 1422
TaaI ACNGT 6 cut(s) 14, 394, 871, 972, 1084, 1382
TaqI TCGA 5 cut(s) 209, 438, 671, 1055, 1416
TatI WGTACW 3 cut(s) 14, 1030, 1296
TauI GCSGC 1 cut(s) 1477
TfiI GAWTC 1 cut(s) 800
Tru1I TTAA 4 cut(s) 342, 372, 747, 1176
Tru9I TTAA 4 cut(s) 342, 372, 747, 1176
TscAI CASTG 1 cut(s) 733
TseFI GTSAC 2 cut(s) 301, 1154
TseI GCWGC 1 cut(s) 1048
Tsp45I GTSAC 2 cut(s) 301, 1154
TspDTI ATGAA 4 cut(s) 395, 645, 1092, 1317
TspGWI ACGGA 1 cut(s) 857
TspRI CASTG 1 cut(s) 733
Vha464I CTTAAG 1 cut(s) 371
VpaK11BI GGWCC 2 cut(s) 451, 884
XapI RAATTY 3 cut(s) 964, 1088, 1186
XceI RCATGY 1 cut(s) 726
XmiI GTMKAC 1 cut(s) 1356
XspI CTAG 3 cut(s) 351, 360, 714
ZrmI AGTACT 1 cut(s) 16
Zsp2I ATGCAT 3 cut(s) 63, 685, 724
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.