Rmu_ssc0000410.1_g000018

DTW

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000410.1
OG0012344
Physical Location & Seq
Forward (+)
78451 .. 79487
1037 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000410.1_g000018.1.cds

Sequence Viewer

Length: 714 bp
atgtccggttcattatccggtcgcttgacccggattagaaccggaatggttcaaagggtgcatcttttgatggggctgtggaaactagagagacccagaaggtggattctgggtgatatgagtagtgctcaaaatcaattaggaggtgtggattctacagaaagttttagtgtaccaaccggtgatgcattacttgtagagcaagaacatgaattcactagtgaaagcgtgtttgctttggaggcttgtcataagcatttggatcgacaatccaatgtggatgaggcttcaactgctgtgacaatggattcactattcgatgaacacaagttttctggtacagattttgatttggttgaaggtgtcaaagttagtaatgctggaactgttataacggctacaattggcaagaatgaagcaattagcgctagtgaccttgaagcccttgatatcaaggtcaagaatctgattgtcttggatggaacatggtcaaaggcaaagagaatgtatgtggaaaacccctggttgaagctattgccacacttgaggttggatttgcacaagctgagcttgtacaatgatgttcgagtccagcccaagccgggatttctttccaccattaagagcattgtgtacgcactaaaagcggtaggggacagtctcgaaggcctagacaatctcttggatgtttttgagtccatggttggagactag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DTW

Protein Analysis

237

Amino Acids

26.2

Weight (kDa)

5.39

Isoelectric Point (pI)

35.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012344)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G54880
fragaria_vesca FvH4_3g22471
malus_domestica MD03G1204100.v1.1 MD11G1220900.v1.1
prunus_persica Prupe.4G211300_v2.0.a1
pyrus_communis pycom03g15470 pycom11g19490
rosa_chinensis RchiOBHm_Chr5g0039141
rosa_laevigata RLG00000033897
rosa_multiflora Rmu_sc0000533.1_g000008 Rmu_ssc0000410.1_g000018 Rmu_ssc0000410.1_g000044
rosa_roxburghii Rroxscaffold_1G00040360
rosa_rugosa Rorug05G0177000
rosa_samantha Rh5BG268300 Rh5CG301100 Rh5DG276100
rosa_wichuraiana Rw0G001970 Rw5G024630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 392
AccB7I CCANNNNNTGG 1 cut(s) 102
AciI CCGC 1 cut(s) 647
AclWI GGATC 1 cut(s) 270
AcsI RAATTY 1 cut(s) 212
AfaI GTAC 4 cut(s) 174, 340, 575, 635
AfeI AGCGCT 1 cut(s) 427
AfiI CCNNNNNNNGG 2 cut(s) 102, 602
AgeI ACCGGT 1 cut(s) 179
AgsI TTSAA 5 cut(s) 53, 291, 359, 440, 529
AhlI ACTAGT 1 cut(s) 218
AjnI CCWGG 1 cut(s) 521
AluBI AGCT 3 cut(s) 532, 565, 570
AluI AGCT 3 cut(s) 532, 565, 570
Alw21I GWGCWC 1 cut(s) 130
Alw26I GTCTC 3 cut(s) 85, 665, 702
AlwI GGATC 1 cut(s) 270
Aor51HI AGCGCT 1 cut(s) 427
AoxI GGCC 1 cut(s) 667
ApoI RAATTY 1 cut(s) 212
AsiGI ACCGGT 1 cut(s) 179
AspLEI GCGC 1 cut(s) 428
AsuC2I CCSGG 2 cut(s) 31, 603
AsuHPI GGTGA 2 cut(s) 125, 194
Bbv12I GWGCWC 1 cut(s) 130
BccI CCATC 2 cut(s) 64, 473
BceAI ACGGC 1 cut(s) 411
BcgI CGANNNNNNTGC 2 cut(s) 245, 279
BciT130I CCWGG 1 cut(s) 523
BcnI CCSGG 2 cut(s) 31, 603
BcoDI GTCTC 3 cut(s) 85, 665, 702
BcuI ACTAGT 1 cut(s) 218
BfaI CTAG 5 cut(s) 86, 219, 429, 671, 712
BfmI CTRYAG 1 cut(s) 156
BfoI RGCGCY 1 cut(s) 429
BlpI GCTNAGC 1 cut(s) 566
Bme1390I CCNGG 3 cut(s) 31, 523, 603
BmrFI CCNGG 3 cut(s) 31, 523, 603
BmsI GCATC 2 cut(s) 70, 175
BplI GAGNNNNNCTC 2 cut(s) 112, 144
Bpu1102I GCTNAGC 1 cut(s) 566
BpuEI CTTGAG 1 cut(s) 565
BpuMI CCSGG 2 cut(s) 31, 603
BsaI GGTCTC 1 cut(s) 85
BsaJI CCNNGG 2 cut(s) 521, 699
BsaWI WCCGGW 4 cut(s) 5, 17, 41, 179
Bsc4I CCNNNNNNNGG 2 cut(s) 102, 602
Bse118I RCCGGY 1 cut(s) 179
BseBI CCWGG 1 cut(s) 523
BseDI CCNNGG 2 cut(s) 521, 699
BseGI GGATG 3 cut(s) 286, 484, 691
BseLI CCNNNNNNNGG 2 cut(s) 102, 602
BseMII CTCAG 1 cut(s) 557
Bsh1285I CGRYCG 1 cut(s) 22
BshFI GGCC 1 cut(s) 669
BshTI ACCGGT 1 cut(s) 179
BsiEI CGRYCG 1 cut(s) 22
BsiHKAI GWGCWC 1 cut(s) 130
BsiSI CCGG 6 cut(s) 6, 18, 31, 42, 180, 602
BslFI GGGAC 1 cut(s) 668
BslI CCNNNNNNNGG 2 cut(s) 102, 602
BsmAI GTCTC 3 cut(s) 85, 665, 702
BsmFI GGGAC 1 cut(s) 668
BsnI GGCC 1 cut(s) 669
Bso31I GGTCTC 1 cut(s) 85
Bsp1286I GDGCHC 1 cut(s) 130
Bsp1407I TGTACA 1 cut(s) 573
Bsp143I GATC 1 cut(s) 262
Bsp1720I GCTNAGC 1 cut(s) 566
Bsp19I CCATGG 1 cut(s) 699
BspACI CCGC 1 cut(s) 647
BspANI GGCC 1 cut(s) 669
BspCNI CTCAG 1 cut(s) 558
BspPI GGATC 1 cut(s) 270
BspTNI GGTCTC 1 cut(s) 85
BsrFI RCCGGY 1 cut(s) 179
BsrGI TGTACA 1 cut(s) 573
BssAI RCCGGY 1 cut(s) 179
BssECI CCNNGG 2 cut(s) 521, 699
BssMI GATC 1 cut(s) 262
BssT1I CCWWGG 1 cut(s) 699
Bst2UI CCWGG 1 cut(s) 523
Bst4CI ACNGT 2 cut(s) 388, 659
BstAUI TGTACA 1 cut(s) 573
BstDEI CTNAG 1 cut(s) 566
BstDSI CCRYGG 1 cut(s) 699
BstF5I GGATG 3 cut(s) 286, 484, 691
BstH2I RGCGCY 1 cut(s) 429
BstHHI GCGC 1 cut(s) 428
BstKTI GATC 1 cut(s) 265
BstMAI GTCTC 3 cut(s) 85, 665, 702
BstMBI GATC 1 cut(s) 262
BstMCI CGRYCG 1 cut(s) 22
BstMWI GCNNNNNNNGC 4 cut(s) 242, 293, 425, 644
BstNI CCWGG 1 cut(s) 523
BstSCI CCNGG 3 cut(s) 29, 521, 601
BstSFI CTRYAG 1 cut(s) 156
BsuRI GGCC 1 cut(s) 669
BtgI CCRYGG 1 cut(s) 699
BtsCI GGATG 3 cut(s) 286, 484, 691
CfoI GCGC 1 cut(s) 428
Cfr10I RCCGGY 1 cut(s) 179
Csp6I GTAC 4 cut(s) 173, 339, 574, 634
CspAI ACCGGT 1 cut(s) 179
CviAII CATG 3 cut(s) 209, 486, 700
CviQI GTAC 4 cut(s) 173, 339, 574, 634
DdeI CTNAG 1 cut(s) 566
DpnI GATC 1 cut(s) 264
DpnII GATC 1 cut(s) 262
Eco130I CCWWGG 1 cut(s) 699
Eco147I AGGCCT 1 cut(s) 669
Eco31I GGTCTC 1 cut(s) 85
Eco32I GATATC 1 cut(s) 451
Eco47III AGCGCT 1 cut(s) 427
EcoRI GAATTC 1 cut(s) 212
EcoRII CCWGG 1 cut(s) 521
EcoRV GATATC 1 cut(s) 451
EcoT14I CCWWGG 1 cut(s) 699
EcoT22I ATGCAT 1 cut(s) 190
ErhI CCWWGG 1 cut(s) 699
FaeI CATG 3 cut(s) 212, 489, 703
FaiI YATR 7 cut(s) 119, 210, 252, 392, 487, 510, 701
FalI AAGNNNNNCTT 2 cut(s) 554, 586
FaqI GGGAC 1 cut(s) 668
FatI CATG 3 cut(s) 208, 485, 699
FokI GGATG 3 cut(s) 293, 491, 698
FspBI CTAG 5 cut(s) 86, 219, 429, 671, 712
GlaI GCGC 1 cut(s) 427
HaeII RGCGCY 1 cut(s) 429
HaeIII GGCC 1 cut(s) 669
HapII CCGG 6 cut(s) 6, 18, 31, 42, 180, 602
HhaI GCGC 1 cut(s) 428
Hin1II CATG 3 cut(s) 212, 489, 703
Hin6I GCGC 1 cut(s) 426
HinP1I GCGC 1 cut(s) 426
HinfI GANTC 6 cut(s) 106, 152, 308, 463, 588, 695
HpaII CCGG 6 cut(s) 6, 18, 31, 42, 180, 602
HphI GGTGA 2 cut(s) 125, 194
Hpy166II GTNNAC 2 cut(s) 173, 634
Hpy188I TCNGA 1 cut(s) 468
Hpy188III TCNNGA 2 cut(s) 460, 662
Hpy8I GTNNAC 2 cut(s) 173, 634
HpyAV CCTTC 3 cut(s) 93, 353, 659
HpyCH4III ACNGT 2 cut(s) 388, 659
HpyCH4V TGCA 3 cut(s) 61, 188, 559
HpyF10VI GCNNNNNNNGC 4 cut(s) 242, 293, 425, 644
HpyF3I CTNAG 1 cut(s) 566
Hsp92II CATG 3 cut(s) 212, 489, 703
HspAI GCGC 1 cut(s) 426
Kzo9I GATC 1 cut(s) 262
LweI GCATC 2 cut(s) 70, 175
MaeI CTAG 5 cut(s) 86, 219, 429, 671, 712
MaeIII GTNAC 2 cut(s) 298, 431
MalI GATC 1 cut(s) 264
MboI GATC 1 cut(s) 262
MfeI CAATTG 1 cut(s) 402
MhlI GDGCHC 1 cut(s) 130
MluCI AATT 4 cut(s) 137, 212, 402, 420
MlyI GAGTC 2 cut(s) 597, 704
MmeI TCCRAC 2 cut(s) 531, 685
MnlI CCTC 4 cut(s) 137, 235, 277, 540
Mph1103I ATGCAT 1 cut(s) 190
MseI TTAA 1 cut(s) 621
MspI CCGG 6 cut(s) 6, 18, 31, 42, 180, 602
MspR9I CCNGG 3 cut(s) 31, 523, 603
MunI CAATTG 1 cut(s) 402
MvaI CCWGG 1 cut(s) 523
MwoI GCNNNNNNNGC 4 cut(s) 242, 293, 425, 644
NciI CCSGG 2 cut(s) 31, 603
NcoI CCATGG 1 cut(s) 699
NdeII GATC 1 cut(s) 262
NlaIII CATG 3 cut(s) 212, 489, 703
NmuCI GTSAC 2 cut(s) 298, 431
NsiI ATGCAT 1 cut(s) 190
PceI AGGCCT 1 cut(s) 669
PfeI GAWTC 4 cut(s) 106, 152, 308, 463
PflMI CCANNNNNTGG 1 cut(s) 102
PinAI ACCGGT 1 cut(s) 179
PleI GAGTC 2 cut(s) 596, 703
PpsI GAGTC 2 cut(s) 596, 703
PsiI TTATAA 1 cut(s) 392
Psp6I CCWGG 1 cut(s) 521
PspGI CCWGG 1 cut(s) 521
RsaI GTAC 4 cut(s) 174, 340, 575, 635
RsaNI GTAC 4 cut(s) 173, 339, 574, 634
SaqAI TTAA 1 cut(s) 621
Sau3AI GATC 1 cut(s) 262
SchI GAGTC 2 cut(s) 597, 704
ScrFI CCNGG 3 cut(s) 31, 523, 603
SduI GDGCHC 1 cut(s) 130
SetI ASST 9 cut(s) 104, 148, 364, 438, 459, 534, 551, 567, 572
SfaNI GCATC 2 cut(s) 70, 175
SfcI CTRYAG 1 cut(s) 156
SmlI CTYRAG 1 cut(s) 544
SmoI CTYRAG 1 cut(s) 544
SpeI ACTAGT 1 cut(s) 218
Sse9I AATT 4 cut(s) 137, 212, 402, 420
SseBI AGGCCT 1 cut(s) 669
SsiI CCGC 1 cut(s) 647
SspMI CTAG 5 cut(s) 86, 219, 429, 671, 712
StuI AGGCCT 1 cut(s) 669
StyD4I CCNGG 3 cut(s) 29, 521, 601
StyI CCWWGG 1 cut(s) 699
TaaI ACNGT 2 cut(s) 388, 659
TaqI TCGA 4 cut(s) 265, 318, 586, 663
TasI AATT 4 cut(s) 137, 212, 402, 420
TatI WGTACW 1 cut(s) 573
TfiI GAWTC 4 cut(s) 106, 152, 308, 463
Tru1I TTAA 1 cut(s) 621
Tru9I TTAA 1 cut(s) 621
TseFI GTSAC 2 cut(s) 298, 431
Tsp45I GTSAC 2 cut(s) 298, 431
TspDTI ATGAA 3 cut(s) 225, 336, 429
Van91I CCANNNNNTGG 1 cut(s) 102
XapI RAATTY 1 cut(s) 212
XspI CTAG 5 cut(s) 86, 219, 429, 671, 712
Zsp2I ATGCAT 1 cut(s) 190
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.