Rh5DG276100

DTW

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0012344
Physical Location & Seq
Forward (+)
33802937 .. 33804268
1332 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG276100.1

Sequence Viewer

Length: 1332 bp
ATGGTGATTCCTACTCAGGCCATTTCCAAGAGACCCATTTGCCCTTCCTGCTCCAAACCCATCCGGCTCTGCCTCTGCAATAGGATCCACAACCCGGGGCTCCACAACTCTGTAAGTGTGACCATTCTCCAACACAGCTTTGAGAAGAACCAGCCTCTTAATTCAGCTAGGATTGCCAGATTAGGGCTTAAGAATCTGAAAGTGGTTACTGTTTTTGATGTACATTTTGATGCCCGGATCGCTATCCGGTTGCTCGACCCGGATTTGGAAACGGGTTTGGACCGGAATGGTTCAAAGGGTGCATCTTTTGATGGAGCTGCGGAAACTGGAGAGACCCATAAGGTGGATTCTGGGTTTTGTGAGTCGGAAATTGGGGGTTTTGAAAATTCATCAAAACAGAGAGGGTGTAGTAGTGTAGTTGATTGTAATGAGCAATGTAGTTTTGGGGATGATATAGGGGATATGAGTAGTGCTCAAAATCAATTAGGAAGTGTAGATTCTGCAGAAAATTGTAGTGTATCGAACGGTGATGCATTGCTTGTAGAGCAAGAAGATGAATTCATTAGTGAAGCATTCACTGATGGAAAGAGTGTGCTTGCTTTGGAGACTTGCCATATGCATTTGGATGGACAATCCAATGTGGATGAGGCTTCAACTGCTACGACTTTGGATTCATTATCTGATAGAGATTTTAATTCAGTTGAAGGTGTCAAAGTTAGTAATGCTGGACCTGTTATAACTGCTACAATTGGCAAGAATGGTGTCATTAGCTCTCTTACTCATACTTGGATGCCACAAACCCATCACAAGAATTCACAATTTGATACGATCTCAGACATTACAGAAGCGCGTGAGGCTCTGGCAAAGGGATTCGTTGTACAGAAGTTGCAAAAGAAGGCACATAAAGGAAATGTAGAGTTAGATGAAGCTCTAGAGTTTGAAGTACAAGTTCCTCCGGGGTCTGTTCTCTTGTTTCCATCCAAAGAAGCAGTTAGTGCTAGTGACCTTGAAGCCATGGATATTAAGGTGAAGAATTTGATTGTCTTGGATGGAACATGGTCAAAGGCAAAGAGAATGTATGTGGAAAACCCCTGGTTGAAGCTATTGCCACACTTGAGGTTGGATTTGGACAAGCTGAGCTTGTACAATGAAGTGAGAGTCCAGCCCAAGCCGGGATTTCTGTCCACCATTGAGAGCATTGTATACGCACTAAAAGCGGTAGGGGACAGTCCCGAAGGGCTGGACAATCTCTTGGATGTTTTCGAGTCCATGGTTGGCGACCAGAGACGATGTAAAGAAGAGAGATTGAGCAATGTCTCGCCGCCCGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DTW

Protein Analysis

443

Amino Acids

48.19

Weight (kDa)

5.1

Isoelectric Point (pI)

33.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DTW PF03942 11 - 76 1.1e-08 DTW domain
DTW PF03942 262 - 428 8.2e-29 DTW domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012344)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G54880
fragaria_vesca FvH4_3g22471
malus_domestica MD03G1204100.v1.1 MD11G1220900.v1.1
prunus_persica Prupe.4G211300_v2.0.a1
pyrus_communis pycom03g15470 pycom11g19490
rosa_chinensis RchiOBHm_Chr5g0039141
rosa_laevigata RLG00000033897
rosa_multiflora Rmu_sc0000533.1_g000008 Rmu_ssc0000410.1_g000018 Rmu_ssc0000410.1_g000044
rosa_roxburghii Rroxscaffold_1G00040360
rosa_rugosa Rorug05G0177000
rosa_samantha Rh5BG268300 Rh5CG301100 Rh5DG276100
rosa_wichuraiana Rw0G001970 Rw5G024630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 737
AccB7I CCANNNNNTGG 1 cut(s) 343
AccI GTMKAC 1 cut(s) 1203
AccII CGCG 1 cut(s) 850
AciI CCGC 3 cut(s) 320, 1217, 1322
AclWI GGATC 3 cut(s) 79, 92, 245
AcsI RAATTY 4 cut(s) 385, 557, 811, 1033
AfaI GTAC 4 cut(s) 222, 879, 945, 1145
AfiI CCNNNNNNNGG 5 cut(s) 94, 183, 265, 343, 1172
AflII CTTAAG 1 cut(s) 188
AgsI TTSAA 7 cut(s) 294, 383, 654, 704, 941, 1010, 1099
AjnI CCWGG 1 cut(s) 1091
AluBI AGCT 8 cut(s) 138, 167, 317, 771, 929, 1102, 1135, 1140
AluI AGCT 8 cut(s) 138, 167, 317, 771, 929, 1102, 1135, 1140
Alw21I GWGCWC 1 cut(s) 475
Alw26I GTCTC 5 cut(s) 25, 326, 599, 1279, 1321
AlwI GGATC 3 cut(s) 79, 92, 245
Ama87I CYCGRG 1 cut(s) 94
AoxI GGCC 1 cut(s) 18
ApeKI GCWGC 1 cut(s) 317
ApoI RAATTY 4 cut(s) 385, 557, 811, 1033
AspLEI GCGC 1 cut(s) 850
AspS9I GGNCC 2 cut(s) 280, 728
AsuC2I CCSGG 7 cut(s) 95, 96, 235, 260, 957, 1173, 1326
AsuHPI GGTGA 3 cut(s) 16, 539, 1039
AvaI CYCGRG 1 cut(s) 94
AvaII GGWCC 2 cut(s) 280, 728
BamHI GGATCC 1 cut(s) 84
BanII GRGCYC 1 cut(s) 102
Bbv12I GWGCWC 1 cut(s) 475
BbvI GCAGC 1 cut(s) 304
BccI CCATC 7 cut(s) 68, 305, 575, 620, 810, 985, 1043
BciT130I CCWGG 1 cut(s) 1093
BcnI CCSGG 7 cut(s) 95, 96, 235, 260, 957, 1173, 1326
BcoDI GTCTC 5 cut(s) 25, 326, 599, 1279, 1321
BfaI CTAG 3 cut(s) 168, 932, 999
BfmI CTRYAG 1 cut(s) 501
BfrI CTTAAG 1 cut(s) 188
BisI GCNGC 2 cut(s) 318, 1322
BlpI GCTNAGC 1 cut(s) 1136
BlsI GCNGC 2 cut(s) 319, 1323
Bme1390I CCNGG 8 cut(s) 95, 96, 235, 260, 957, 1093, 1173, 1326
Bme18I GGWCC 2 cut(s) 280, 728
BmeT110I CYCGRG 1 cut(s) 94
BmgT120I GGNCC 2 cut(s) 280, 728
BmiI GGNNCC 2 cut(s) 86, 101
BmrFI CCNGG 8 cut(s) 95, 96, 235, 260, 957, 1093, 1173, 1326
BmsI GCATC 4 cut(s) 220, 311, 520, 780
BplI GAGNNNNNCTC 2 cut(s) 457, 489
BpmI CTGGAG 1 cut(s) 348
Bpu1102I GCTNAGC 1 cut(s) 1136
BpuEI CTTGAG 1 cut(s) 1135
BpuMI CCSGG 7 cut(s) 95, 96, 235, 260, 957, 1173, 1326
BsaBI GATNNNNATC 1 cut(s) 242
BsaI GGTCTC 2 cut(s) 25, 326
BsaJI CCNNGG 6 cut(s) 94, 95, 956, 1014, 1091, 1269
BsaWI WCCGGW 2 cut(s) 246, 282
Bsc4I CCNNNNNNNGG 5 cut(s) 94, 183, 265, 343, 1172
Bse1I ACTGG 1 cut(s) 331
Bse3DI GCAATG 3 cut(s) 440, 533, 1318
Bse8I GATNNNNATC 1 cut(s) 242
BseBI CCWGG 1 cut(s) 1093
BseDI CCNNGG 6 cut(s) 94, 95, 956, 1014, 1091, 1269
BseGI GGATG 8 cut(s) 60, 454, 631, 649, 795, 977, 1054, 1261
BseJI GATNNNNATC 1 cut(s) 242
BseLI CCNNNNNNNGG 5 cut(s) 94, 183, 265, 343, 1172
BseMI GCAATG 3 cut(s) 440, 533, 1318
BseMII CTCAG 3 cut(s) 29, 846, 1127
BseNI ACTGG 1 cut(s) 331
BseXI GCAGC 1 cut(s) 304
Bsh1236I CGCG 1 cut(s) 850
BshFI GGCC 1 cut(s) 20
BsiHKAI GWGCWC 1 cut(s) 475
BsiHKCI CYCGRG 1 cut(s) 94
BsiSI CCGG 9 cut(s) 64, 95, 235, 247, 260, 283, 956, 1172, 1326
BslFI GGGAC 2 cut(s) 1215, 1238
BslI CCNNNNNNNGG 5 cut(s) 94, 183, 265, 343, 1172
BsmAI GTCTC 5 cut(s) 25, 326, 599, 1279, 1321
BsmBI CGTCTC 1 cut(s) 1279
BsmFI GGGAC 2 cut(s) 1215, 1238
BsmI GAATGC 1 cut(s) 572
BsnI GGCC 1 cut(s) 20
Bso31I GGTCTC 2 cut(s) 25, 326
BsoBI CYCGRG 1 cut(s) 94
Bsp1286I GDGCHC 2 cut(s) 102, 475
Bsp1407I TGTACA 3 cut(s) 220, 877, 1143
Bsp143I GATC 3 cut(s) 84, 237, 828
Bsp1720I GCTNAGC 1 cut(s) 1136
Bsp19I CCATGG 2 cut(s) 1014, 1269
BspACI CCGC 3 cut(s) 320, 1217, 1322
BspANI GGCC 1 cut(s) 20
BspCNI CTCAG 3 cut(s) 28, 845, 1128
BspFNI CGCG 1 cut(s) 850
BspLI GGNNCC 2 cut(s) 86, 101
BspMAI CTGCAG 1 cut(s) 505
BspPI GGATC 3 cut(s) 79, 92, 245
BspTI CTTAAG 1 cut(s) 188
BspTNI GGTCTC 2 cut(s) 25, 326
BsrDI GCAATG 3 cut(s) 440, 533, 1318
BsrGI TGTACA 3 cut(s) 220, 877, 1143
BsrI ACTGG 1 cut(s) 331
BssECI CCNNGG 6 cut(s) 94, 95, 956, 1014, 1091, 1269
BssMI GATC 3 cut(s) 84, 237, 828
BssNAI GTATAC 1 cut(s) 1204
BssT1I CCWWGG 2 cut(s) 1014, 1269
Bst1107I GTATAC 1 cut(s) 1204
Bst2UI CCWGG 1 cut(s) 1093
Bst4CI ACNGT 3 cut(s) 211, 527, 1229
Bst6I CTCTTC 1 cut(s) 1293
BstAFI CTTAAG 1 cut(s) 188
BstAPI GCANNNNNTGC 1 cut(s) 995
BstAUI TGTACA 3 cut(s) 220, 877, 1143
BstC8I GCNNGC 1 cut(s) 597
BstDEI CTNAG 3 cut(s) 15, 832, 1136
BstDSI CCRYGG 2 cut(s) 1014, 1269
BstF5I GGATG 8 cut(s) 60, 454, 631, 649, 795, 977, 1054, 1261
BstFNI CGCG 1 cut(s) 850
BstHHI GCGC 1 cut(s) 850
BstKTI GATC 3 cut(s) 87, 240, 831
BstMAI GTCTC 5 cut(s) 25, 326, 599, 1279, 1321
BstMBI GATC 3 cut(s) 84, 237, 828
BstMWI GCNNNNNNNGC 8 cut(s) 48, 173, 239, 544, 656, 854, 995, 1214
BstNI CCWGG 1 cut(s) 1093
BstSCI CCNGG 8 cut(s) 93, 94, 233, 258, 955, 1091, 1171, 1324
BstSFI CTRYAG 1 cut(s) 501
BstUI CGCG 1 cut(s) 850
BstV1I GCAGC 1 cut(s) 304
BstX2I RGATCY 1 cut(s) 84
BstYI RGATCY 1 cut(s) 84
BstZ17I GTATAC 1 cut(s) 1204
BsuRI GGCC 1 cut(s) 20
BtgI CCRYGG 2 cut(s) 1014, 1269
BtsCI GGATG 8 cut(s) 60, 454, 631, 649, 795, 977, 1054, 1261
BtsIMutI CAGTG 1 cut(s) 576
Cac8I GCNNGC 1 cut(s) 597
CfoI GCGC 1 cut(s) 850
Cfr13I GGNCC 2 cut(s) 280, 728
Cfr9I CCCGGG 1 cut(s) 94
Csp6I GTAC 4 cut(s) 221, 878, 944, 1144
CviAII CATG 3 cut(s) 1015, 1056, 1270
CviQI GTAC 4 cut(s) 221, 878, 944, 1144
DdeI CTNAG 3 cut(s) 15, 832, 1136
DpnI GATC 3 cut(s) 86, 239, 830
DpnII GATC 3 cut(s) 84, 237, 828
Eam1104I CTCTTC 1 cut(s) 1293
EarI CTCTTC 1 cut(s) 1293
Eco130I CCWWGG 2 cut(s) 1014, 1269
Eco24I GRGCYC 1 cut(s) 102
Eco31I GGTCTC 2 cut(s) 25, 326
Eco47I GGWCC 2 cut(s) 280, 728
Eco88I CYCGRG 1 cut(s) 94
EcoRI GAATTC 2 cut(s) 557, 811
EcoRII CCWGG 1 cut(s) 1091
EcoT14I CCWWGG 2 cut(s) 1014, 1269
EcoT22I ATGCAT 2 cut(s) 535, 621
EcoT38I GRGCYC 1 cut(s) 102
ErhI CCWWGG 2 cut(s) 1014, 1269
Esp3I CGTCTC 1 cut(s) 1279
FaeI CATG 3 cut(s) 1018, 1059, 1273
FalI AAGNNNNNCTT 2 cut(s) 1124, 1156
FaqI GGGAC 2 cut(s) 1215, 1238
FatI CATG 3 cut(s) 1014, 1055, 1269
FauNDI CATATG 1 cut(s) 615
FblI GTMKAC 1 cut(s) 1203
Fnu4HI GCNGC 2 cut(s) 318, 1322
FokI GGATG 8 cut(s) 47, 461, 638, 656, 802, 964, 1061, 1268
FriOI GRGCYC 1 cut(s) 102
Fsp4HI GCNGC 2 cut(s) 318, 1322
FspBI CTAG 3 cut(s) 168, 932, 999
GlaI GCGC 1 cut(s) 849
GluI GCNGC 2 cut(s) 318, 1322
GsuI CTGGAG 1 cut(s) 348
HaeIII GGCC 1 cut(s) 20
HapII CCGG 9 cut(s) 64, 95, 235, 247, 260, 283, 956, 1172, 1326
HhaI GCGC 1 cut(s) 850
Hin1II CATG 3 cut(s) 1018, 1059, 1273
Hin6I GCGC 1 cut(s) 848
HinP1I GCGC 1 cut(s) 848
HinfI GANTC 9 cut(s) 7, 193, 347, 362, 497, 671, 870, 1158, 1265
HpaII CCGG 9 cut(s) 64, 95, 235, 247, 260, 283, 956, 1172, 1326
HphI GGTGA 3 cut(s) 16, 539, 1039
Hpy166II GTNNAC 2 cut(s) 1185, 1204
Hpy188I TCNGA 4 cut(s) 198, 367, 682, 835
Hpy188III TCNNGA 2 cut(s) 932, 1232
Hpy8I GTNNAC 2 cut(s) 1185, 1204
HpyAV CCTTC 4 cut(s) 54, 698, 889, 1229
HpyCH4III ACNGT 3 cut(s) 211, 527, 1229
HpyCH4V TGCA 6 cut(s) 78, 302, 503, 533, 619, 889
HpyF10VI GCNNNNNNNGC 8 cut(s) 48, 173, 239, 544, 656, 854, 995, 1214
HpyF3I CTNAG 3 cut(s) 15, 832, 1136
Hsp92II CATG 3 cut(s) 1018, 1059, 1273
HspAI GCGC 1 cut(s) 848
Kzo9I GATC 3 cut(s) 84, 237, 828
LmnI GCTCC 3 cut(s) 56, 105, 314
Lsp1109I GCAGC 1 cut(s) 304
LweI GCATC 4 cut(s) 220, 311, 520, 780
MaeI CTAG 3 cut(s) 168, 932, 999
MaeIII GTNAC 3 cut(s) 118, 205, 1001
MalI GATC 3 cut(s) 86, 239, 830
MboI GATC 3 cut(s) 84, 237, 828
MboII GAAGA 4 cut(s) 157, 563, 1042, 1310
MfeI CAATTG 1 cut(s) 747
MflI RGATCY 1 cut(s) 84
MhlI GDGCHC 2 cut(s) 102, 475
MlyI GAGTC 3 cut(s) 371, 1167, 1274
MmeI TCCRAC 3 cut(s) 154, 345, 1101
MnlI CCTC 7 cut(s) 83, 165, 395, 640, 847, 963, 1110
Mph1103I ATGCAT 2 cut(s) 535, 621
MseI TTAA 4 cut(s) 159, 189, 693, 1023
MslI CAYNNNNRTG 2 cut(s) 228, 624
MspCI CTTAAG 1 cut(s) 188
MspI CCGG 9 cut(s) 64, 95, 235, 247, 260, 283, 956, 1172, 1326
MspR9I CCNGG 8 cut(s) 95, 96, 235, 260, 957, 1093, 1173, 1326
MunI CAATTG 1 cut(s) 747
Mva1269I GAATGC 1 cut(s) 572
MvaI CCWGG 1 cut(s) 1093
MvnI CGCG 1 cut(s) 850
MwoI GCNNNNNNNGC 8 cut(s) 48, 173, 239, 544, 656, 854, 995, 1214
NciI CCSGG 7 cut(s) 95, 96, 235, 260, 957, 1173, 1326
NcoI CCATGG 2 cut(s) 1014, 1269
NdeI CATATG 1 cut(s) 615
NdeII GATC 3 cut(s) 84, 237, 828
NlaIII CATG 3 cut(s) 1018, 1059, 1273
NlaIV GGNNCC 2 cut(s) 86, 101
NmuCI GTSAC 2 cut(s) 118, 1001
NsiI ATGCAT 2 cut(s) 535, 621
PctI GAATGC 1 cut(s) 572
PfeI GAWTC 6 cut(s) 7, 193, 347, 497, 671, 870
PflMI CCANNNNNTGG 1 cut(s) 343
PkrI GCNGC 2 cut(s) 319, 1323
PleI GAGTC 3 cut(s) 370, 1166, 1273
PpsI GAGTC 3 cut(s) 370, 1166, 1273
PsiI TTATAA 1 cut(s) 737
Psp6I CCWGG 1 cut(s) 1091
PspGI CCWGG 1 cut(s) 1091
PspN4I GGNNCC 2 cut(s) 86, 101
PspPI GGNCC 2 cut(s) 280, 728
PstI CTGCAG 1 cut(s) 505
PsuI RGATCY 1 cut(s) 84
RsaI GTAC 4 cut(s) 222, 879, 945, 1145
RsaNI GTAC 4 cut(s) 221, 878, 944, 1144
RseI CAYNNNNRTG 2 cut(s) 228, 624
SaqAI TTAA 4 cut(s) 159, 189, 693, 1023
SatI GCNGC 2 cut(s) 318, 1322
Sau3AI GATC 3 cut(s) 84, 237, 828
Sau96I GGNCC 2 cut(s) 280, 728
SchI GAGTC 3 cut(s) 371, 1167, 1274
ScrFI CCNGG 8 cut(s) 95, 96, 235, 260, 957, 1093, 1173, 1326
SduI GDGCHC 2 cut(s) 102, 475
SfaNI GCATC 4 cut(s) 220, 311, 520, 780
SfcI CTRYAG 1 cut(s) 501
SinI GGWCC 2 cut(s) 280, 728
SmaI CCCGGG 1 cut(s) 96
SmiMI CAYNNNNRTG 2 cut(s) 228, 624
SmlI CTYRAG 2 cut(s) 188, 1114
SmoI CTYRAG 2 cut(s) 188, 1114
SsiI CCGC 3 cut(s) 320, 1217, 1322
SspMI CTAG 3 cut(s) 168, 932, 999
StyD4I CCNGG 8 cut(s) 93, 94, 233, 258, 955, 1091, 1171, 1324
StyI CCWWGG 2 cut(s) 1014, 1269
TaaI ACNGT 3 cut(s) 211, 527, 1229
TaqI TCGA 3 cut(s) 255, 521, 1263
TatI WGTACW 4 cut(s) 220, 877, 943, 1143
TauI GCSGC 1 cut(s) 1324
TfiI GAWTC 6 cut(s) 7, 193, 347, 497, 671, 870
Tru1I TTAA 4 cut(s) 159, 189, 693, 1023
Tru9I TTAA 4 cut(s) 159, 189, 693, 1023
TscAI CASTG 1 cut(s) 583
TseFI GTSAC 2 cut(s) 118, 1001
TseI GCWGC 1 cut(s) 317
Tsp45I GTSAC 2 cut(s) 118, 1001
TspDTI ATGAA 6 cut(s) 378, 550, 570, 663, 939, 1164
TspMI CCCGGG 1 cut(s) 94
TspRI CASTG 1 cut(s) 583
Van91I CCANNNNNTGG 1 cut(s) 343
Vha464I CTTAAG 1 cut(s) 188
VpaK11BI GGWCC 2 cut(s) 280, 728
XapI RAATTY 4 cut(s) 385, 557, 811, 1033
XbaI TCTAGA 1 cut(s) 931
XmaI CCCGGG 1 cut(s) 94
XmiI GTMKAC 1 cut(s) 1203
XspI CTAG 3 cut(s) 168, 932, 999
Zsp2I ATGCAT 2 cut(s) 535, 621
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.