MD11G1220900.v1.1

DTW

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0012344
Physical Location & Seq
Reverse (-)
32392279 .. 32396709
4431 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1220900.v1.1.491

Sequence Viewer

Length: 2409 bp
ATGGCTACTCGAACACATAGACGCTGGTTCGGTTCTCGGACCCGAAACAACAATCTCTGCTCACTACTCCAAAACCCTTGCTTTCTTCCTCAAACCCTAAAAATGGTGACTACTCAGGCCGCTTCCAAACGACCCATTTGCCCATCGTGCTCCAAACCGGTCCGGCTCTGCCTCTGCACTCGGATTCAGAACCCGGGGCTCCATAACACTGTAAGTGTGACCATTCTTCAACACAGCTTAGAAAAGAACCACCCACTTAATTCCGCTAGAATTGCTAGATTAGGCCTCAAGAATCTCAGTGTAGTTACTGTTTCTGATGTGAATTTTGAATCCCGGTTCGTTATCCGGCCGCCGGACTCGGTTTCTCAAATGGGTTCGGACCGGAATGGTTTGAAAAGTTTAGGTTTTGATGAAGTTGTTGAAAGTAGAGAGACCCAGAAGCTGGGTTTTGAGTATTGTGATGGGTTCAGCCCAGGGGAAGAAAATGTGGGAAAGTTGGGATTTGGGTTATCTGCAAATTTGGCAGAAGAGAAAAACAATAGTAGTTTGACTGATAGTGAGAAATGCAATTTTGGTAGTGATTTAGGTGATATGAGTAGTTCGCAAAACCGAATAGGGGATGTAGATTTTTTGGAGAAAGAACATGAATTCAGTAGTGCAGATTTCACTGATGGTAGGCACGTAGATGGGCAATGCAATGTCGATGAGGTTTCTGCTTCTACTGCGTTGGATGCATTATCCGGGGATTCTGATGTAGGTTTTGATGCGGTTGAAGGTATCGAAATGGGTAATGGTGATCCTGTTATAACTGCTACCATTGGTAAGCATGGCGTCATTAGCTCACTTTCGCATAATTGGATGCCACAAACTTGTTGCAAGGATCTGAAATTTGATACGATTTTAGACATCGCAGATGCGCGTGGTGCACTAGCAGAGGGGTTTGTTGTACGGAAGTTGCAAAAGCAGCCGGTGAAGGGAAGTGTGGAGTTGGATGAGTATGTGGAGTTTGAGGTAGAGATTCCTCCTGGATCTGTACTCTTATTTCCATCTGAAGAAGCAGTCAGTGTCGGGGACCTTGAGGCGATGGATATTAAGGTGAAGAATCTGATTGTTTTGGATGGAACATGGTCAAAGGCAAAGAGAATGTATGGGGAAAACCCCTGGTTGAAACTCTTGCCGCACTTGAAGTTGAATGTGGACAAGATGAGCTTGTACAGTGAAGTGAGGCTTCAGCCGAAGCCGGGATTTCTCTCCACCATTGAGAGCATTGTGTATGCACTCAAGGCAGTAGGGGACAGCCCTGAGGGGCTGGACAACCTCTTGGATGTTTTCAGGTCTATGGTCGAGGACCAGATACGATGCAAAGAAGAGAGATTAAGCAATGTCTCGCCGGACCCAATCAAACCATTCCTCTCCATCTCCCGCCTTCTCACCCAGACTCTCTCTGACGATCCATACGAATACGACCCTCCGTTCTCTCCGGTTTCCAAACCCCCAAAGCCCAAAAAGAACCTGACTCAGTCTAAAGCTCCGGACCCGAAGAATGACCCGTCCCGGCCTCTCAAATCAGACCTGCCCTTCGACTTCAGCTACTCGTATTCGGAGACCAACCCAGCGGTCGAACCTATTGCTTACCGAGAGTCCCCCACGTTCTCTCCTTTTGGGCCCGGTCGACTTGATCGGAAATGGACGGGCACTGTTGCACCGGCCCAGCAAGAAGTGGACTTGGACCGGGTCGCAGAGGAGAGAAAACGGGTTCTCGGAGACCCACTAACGGAGGAGGAAGCGGCGGAGCTGGTGGAAGGCTGCCGTCATAGTGATTGTTCTCGGAAAATCAGTATGGGAAAGGGAGGAGTTGCACATAACATGTTAGATGATATCCACAACCATTGGAAGCGAGCCGAGGCGGTGAGGATCAAGTGTTTGGGAGTGCCAACTATTGACATGGACAATGTTTGCTTCCACCTTGAGGACAAGTGCGGTGGGAAGATTATTTACCGGCACATTAATGTCCTTATATTATATCGGGGTAGAAACTATGATCCGAAGAATCGATCTGTGATACCTGTAATGCTCTGGAAGCCGTATCCTCCGATATATCCAAAGCTTGTGAAGAATGTTGCTGACGGTTTGACATTTGAAGAAACGGAGTTAAGAAACAGAGGACTAAATTGTCCACCTCTAATGAAACTTATTAGGGAGGCCTTCAATACTGAAGAGGTAGTGAGACAAGACTGCACGCATGTTGGTACCAGCGATTGCAAAAGGATAGGCGTAAAATTAAGGGATCTAGCGCCTTGCATCCCTTTTTTGTTCAAGGATGAGCAGATTATACTTTGGAGGGGGAAGAGAGATCAAGAGCAAGACTCCAAGTGCACAGATAGATATGAAAAATCCACAGTTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DTW

Protein Analysis

803

Amino Acids

89.55

Weight (kDa)

5.8

Isoelectric Point (pI)

44.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DTW PF03942 44 - 107 2.8e-09 DTW domain
DTW PF03942 344 - 451 8.4e-27 DTW domain
CRS1_YhbY PF01985 591 - 676 4.4e-15 CRS1 / YhbY (CRM) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012344)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G54880
fragaria_vesca FvH4_3g22471
malus_domestica MD03G1204100.v1.1 MD11G1220900.v1.1
prunus_persica Prupe.4G211300_v2.0.a1
pyrus_communis pycom03g15470 pycom11g19490
rosa_chinensis RchiOBHm_Chr5g0039141
rosa_laevigata RLG00000033897
rosa_multiflora Rmu_sc0000533.1_g000008 Rmu_ssc0000410.1_g000018 Rmu_ssc0000410.1_g000044
rosa_roxburghii Rroxscaffold_1G00040360
rosa_rugosa Rorug05G0177000
rosa_samantha Rh5BG268300 Rh5CG301100 Rh5DG276100
rosa_wichuraiana Rw0G001970 Rw5G024630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 806
AasI GACNNNNNNGTC 1 cut(s) 2172
Acc36I ACCTGC 1 cut(s) 1581
Acc65I GGTACC 1 cut(s) 2249
AccB1I GGYRCC 1 cut(s) 2249
AccB7I CCANNNNNTGG 1 cut(s) 442
AccI GTMKAC 1 cut(s) 1672
AccII CGCG 1 cut(s) 919
AccIII TCCGGA 1 cut(s) 1531
AclWI GGATC 7 cut(s) 791, 888, 1036, 1445, 1922, 2036, 2295
AcoI YGGCCR 1 cut(s) 347
AcsI RAATTY 4 cut(s) 322, 517, 647, 887
AcuI CTGAAG 4 cut(s) 1071, 1214, 1570, 2235
AcyI GRCGYC 1 cut(s) 831
AfaI GTAC 4 cut(s) 948, 1035, 1214, 2251
AfiI CCNNNNNNNGG 8 cut(s) 103, 352, 442, 616, 974, 1241, 1774, 1969
AflIII ACRYGT 1 cut(s) 1866
AgeI ACCGGT 1 cut(s) 157
AjnI CCWGG 3 cut(s) 472, 1024, 1160
AluBI AGCT 8 cut(s) 237, 442, 840, 1209, 1529, 1590, 1795, 2107
AluI AGCT 8 cut(s) 237, 442, 840, 1209, 1529, 1590, 1795, 2107
Alw21I GWGCWC 3 cut(s) 152, 928, 2378
Alw26I GTCTC 5 cut(s) 425, 1390, 1598, 1758, 2221
Alw44I GTGCAC 2 cut(s) 924, 2374
AlwI GGATC 7 cut(s) 791, 888, 1036, 1445, 1922, 2036, 2295
AlwNI CAGNNNCTG 1 cut(s) 442
Ama87I CYCGRG 1 cut(s) 193
Aor13HI TCCGGA 1 cut(s) 1531
AoxI GGCC 7 cut(s) 117, 283, 347, 1556, 1664, 1707, 2202
ApaI GGGCCC 1 cut(s) 1668
ApaLI GTGCAC 2 cut(s) 924, 2374
ApeKI GCWGC 2 cut(s) 964, 1806
ApoI RAATTY 4 cut(s) 322, 517, 647, 887
ArsI GACNNNNNNTTYG 2 cut(s) 1562, 1594
AseI ATTAAT 1 cut(s) 2007
AsiGI ACCGGT 1 cut(s) 157
Asp718I GGTACC 1 cut(s) 2249
AspLEI GCGC 2 cut(s) 919, 2296
AsuC2I CCSGG 8 cut(s) 194, 195, 334, 742, 1242, 1555, 1668, 1733
AsuHPI GGTGA 7 cut(s) 118, 599, 806, 982, 1108, 1423, 1921
AvaI CYCGRG 1 cut(s) 193
AvaII GGWCC 8 cut(s) 39, 160, 379, 1072, 1348, 1393, 1534, 1729
AxyI CCTNAGG 1 cut(s) 1302
BaeGI GKGCMC 4 cut(s) 928, 1668, 1697, 2378
BanI GGYRCC 1 cut(s) 2249
BanII GRGCYC 2 cut(s) 201, 1668
BarI GAAGNNNNNNTAC 2 cut(s) 1979, 2011
Bbv12I GWGCWC 3 cut(s) 152, 928, 2378
BbvI GCAGC 2 cut(s) 976, 1793
BccI CCATC 8 cut(s) 151, 455, 665, 680, 1054, 1078, 1112, 1424
BceAI ACGGC 2 cut(s) 1794, 2068
BcgI CGANNNNNNTGC 4 cut(s) 120, 154, 1610, 1644
BciT130I CCWGG 3 cut(s) 474, 1026, 1162
BciVI GTATCC 1 cut(s) 2097
BcnI CCSGG 8 cut(s) 194, 195, 334, 742, 1242, 1555, 1668, 1733
BcoDI GTCTC 5 cut(s) 425, 1390, 1598, 1758, 2221
BfaI CTAG 4 cut(s) 267, 276, 929, 2291
BfoI RGCGCY 1 cut(s) 2297
BfuAI ACCTGC 1 cut(s) 1581
BfuI GTATCC 1 cut(s) 2097
BisI GCNGC 6 cut(s) 120, 350, 965, 1178, 1788, 1807
BlsI GCNGC 6 cut(s) 121, 351, 966, 1179, 1789, 1808
Bme18I GGWCC 8 cut(s) 39, 160, 379, 1072, 1348, 1393, 1534, 1729
BmeT110I CYCGRG 1 cut(s) 193
BmiI GGNNCC 7 cut(s) 41, 200, 1073, 1395, 1536, 1666, 2251
BmsI GCATC 6 cut(s) 721, 754, 849, 904, 1349, 2310
BplI GAGNNNNNCTC 2 cut(s) 2351, 2383
BpuEI CTTGAG 4 cut(s) 272, 1097, 1265, 1988
BpuMI CCSGG 8 cut(s) 194, 195, 334, 742, 1242, 1555, 1668, 1733
Bsa29I ATCGAT 1 cut(s) 2053
BsaAI YACGTR 1 cut(s) 682
BsaHI GRCGYC 1 cut(s) 831
BsaI GGTCTC 3 cut(s) 425, 1598, 1758
BsaJI CCNNGG 7 cut(s) 193, 194, 472, 473, 741, 1160, 1902
BsaWI WCCGGW 4 cut(s) 157, 381, 1480, 1531
BsaXI ACNNNNNCTCC 2 cut(s) 1639, 1669
Bsc4I CCNNNNNNNGG 8 cut(s) 103, 352, 442, 616, 974, 1241, 1774, 1969
Bse118I RCCGGY 4 cut(s) 157, 967, 1705, 1998
Bse21I CCTNAGG 1 cut(s) 1302
Bse3DI GCAATG 3 cut(s) 698, 703, 1387
BseAI TCCGGA 1 cut(s) 1531
BseBI CCWGG 3 cut(s) 474, 1026, 1162
BseCI ATCGAT 1 cut(s) 2053
BseDI CCNNGG 7 cut(s) 193, 194, 472, 473, 741, 1160, 1902
BseGI GGATG 8 cut(s) 625, 736, 864, 997, 1123, 1330, 2301, 2326
BseLI CCNNNNNNNGG 8 cut(s) 103, 352, 442, 616, 974, 1241, 1774, 1969
BseMI GCAATG 3 cut(s) 698, 703, 1387
BseMII CTCAG 4 cut(s) 128, 310, 1293, 1532
BseRI GAGGAG 3 cut(s) 1757, 1793, 1866
BseSI GKGCMC 4 cut(s) 928, 1668, 1697, 2378
BseX3I CGGCCG 1 cut(s) 347
BseXI GCAGC 2 cut(s) 976, 1793
BseYI CCCAGC 3 cut(s) 442, 1612, 1710
BsgI GTGCAG 3 cut(s) 160, 678, 2221
Bsh1236I CGCG 1 cut(s) 919
Bsh1285I CGRYCG 3 cut(s) 350, 1620, 1672
BshFI GGCC 7 cut(s) 119, 285, 349, 1558, 1666, 1709, 2204
BshNI GGYRCC 1 cut(s) 2249
BshTI ACCGGT 1 cut(s) 157
BshVI ATCGAT 1 cut(s) 2053
BsiEI CGRYCG 3 cut(s) 350, 1620, 1672
BsiHKAI GWGCWC 3 cut(s) 152, 928, 2378
BsiHKCI CYCGRG 1 cut(s) 193
BslFI GGGAC 4 cut(s) 1085, 1307, 1537, 1627
BslI CCNNNNNNNGG 8 cut(s) 103, 352, 442, 616, 974, 1241, 1774, 1969
BsmAI GTCTC 5 cut(s) 425, 1390, 1598, 1758, 2221
BsmFI GGGAC 4 cut(s) 1085, 1307, 1537, 1627
BsnI GGCC 7 cut(s) 119, 285, 349, 1558, 1666, 1709, 2204
Bso31I GGTCTC 3 cut(s) 425, 1598, 1758
BsoBI CYCGRG 1 cut(s) 193
Bsp120I GGGCCC 1 cut(s) 1664
Bsp1286I GDGCHC 6 cut(s) 152, 201, 928, 1668, 1697, 2378
Bsp13I TCCGGA 1 cut(s) 1531
Bsp1407I TGTACA 1 cut(s) 1212
BspANI GGCC 7 cut(s) 119, 285, 349, 1558, 1666, 1709, 2204
BspCNI CTCAG 4 cut(s) 127, 309, 1294, 1531
BspDI ATCGAT 1 cut(s) 2053
BspEI TCCGGA 1 cut(s) 1531
BspFNI CGCG 1 cut(s) 919
BspLI GGNNCC 7 cut(s) 41, 200, 1073, 1395, 1536, 1666, 2251
BspMI ACCTGC 1 cut(s) 1581
BspPI GGATC 7 cut(s) 791, 888, 1036, 1445, 1922, 2036, 2295
BspT107I GGYRCC 1 cut(s) 2249
BspTNI GGTCTC 3 cut(s) 425, 1598, 1758
BsrDI GCAATG 3 cut(s) 698, 703, 1387
BsrFI RCCGGY 4 cut(s) 157, 967, 1705, 1998
BsrGI TGTACA 1 cut(s) 1212
BssAI RCCGGY 4 cut(s) 157, 967, 1705, 1998
BssECI CCNNGG 7 cut(s) 193, 194, 472, 473, 741, 1160, 1902
BssNI GRCGYC 1 cut(s) 831
Bst2UI CCWGG 3 cut(s) 474, 1026, 1162
Bst4CI ACNGT 6 cut(s) 211, 310, 1217, 1699, 2129, 2401
Bst6I CTCTTC 4 cut(s) 522, 1362, 2211, 2342
BstACI GRCGYC 1 cut(s) 831
BstAUI TGTACA 1 cut(s) 1212
BstBAI YACGTR 1 cut(s) 682
BstC8I GCNNGC 2 cut(s) 1899, 2240
BstDEI CTNAG 5 cut(s) 114, 238, 296, 1302, 1518
BstF5I GGATG 8 cut(s) 625, 736, 864, 997, 1123, 1330, 2301, 2326
BstFNI CGCG 1 cut(s) 919
BstH2I RGCGCY 1 cut(s) 2297
BstHHI GCGC 2 cut(s) 919, 2296
BstMAI GTCTC 5 cut(s) 425, 1390, 1598, 1758, 2221
BstMCI CGRYCG 3 cut(s) 350, 1620, 1672
BstNI CCWGG 3 cut(s) 474, 1026, 1162
BstNSI RCATGY 2 cut(s) 1870, 2246
BstSLI GKGCMC 4 cut(s) 928, 1668, 1697, 2378
BstUI CGCG 1 cut(s) 919
BstV1I GCAGC 2 cut(s) 976, 1793
BstX2I RGATCY 3 cut(s) 880, 1028, 2287
BstYI RGATCY 3 cut(s) 880, 1028, 2287
BstZI CGGCCG 1 cut(s) 347
Bsu15I ATCGAT 1 cut(s) 2053
Bsu36I CCTNAGG 1 cut(s) 1302
BsuI GTATCC 1 cut(s) 2097
BsuRI GGCC 7 cut(s) 119, 285, 349, 1558, 1666, 1709, 2204
BsuTUI ATCGAT 1 cut(s) 2053
BtgZI GCGATG 2 cut(s) 892, 1097
BtsCI GGATG 8 cut(s) 625, 736, 864, 997, 1123, 1330, 2301, 2326
BtsIMutI CAGTG 6 cut(s) 207, 304, 666, 1069, 1222, 1695
BveI ACCTGC 1 cut(s) 1581
Cac8I GCNNGC 2 cut(s) 1899, 2240
CaiI CAGNNNCTG 1 cut(s) 442
CfoI GCGC 2 cut(s) 919, 2296
Cfr10I RCCGGY 4 cut(s) 157, 967, 1705, 1998
Cfr9I CCCGGG 1 cut(s) 193
ClaI ATCGAT 1 cut(s) 2053
CpoI CGGWCCG 2 cut(s) 160, 379
CseI GACGC 2 cut(s) 30, 820
Csp6I GTAC 4 cut(s) 947, 1034, 1213, 2250
CspAI ACCGGT 1 cut(s) 157
CspCI CAANNNNNGTGG 4 cut(s) 1871, 1906, 1963, 1998
CspI CGGWCCG 2 cut(s) 160, 379
CviAII CATG 6 cut(s) 644, 827, 1125, 1867, 1945, 2243
CviQI GTAC 4 cut(s) 947, 1034, 1213, 2250
DdeI CTNAG 5 cut(s) 114, 238, 296, 1302, 1518
DrdI GACNNNNNNGTC 1 cut(s) 2172
DseDI GACNNNNNNGTC 1 cut(s) 2172
EaeI YGGCCR 1 cut(s) 347
EagI CGGCCG 1 cut(s) 347
Eam1104I CTCTTC 4 cut(s) 522, 1362, 2211, 2342
EarI CTCTTC 4 cut(s) 522, 1362, 2211, 2342
EciI GGCGGA 1 cut(s) 1805
EclXI CGGCCG 1 cut(s) 347
Eco147I AGGCCT 2 cut(s) 285, 2204
Eco24I GRGCYC 2 cut(s) 201, 1668
Eco31I GGTCTC 3 cut(s) 425, 1598, 1758
Eco32I GATATC 1 cut(s) 1879
Eco47I GGWCC 8 cut(s) 39, 160, 379, 1072, 1348, 1393, 1534, 1729
Eco52I CGGCCG 1 cut(s) 347
Eco57I CTGAAG 4 cut(s) 1071, 1214, 1570, 2235
Eco81I CCTNAGG 1 cut(s) 1302
Eco88I CYCGRG 1 cut(s) 193
EcoO109I RGGNCCY 1 cut(s) 1072
EcoRI GAATTC 1 cut(s) 647
EcoRII CCWGG 3 cut(s) 472, 1024, 1160
EcoRV GATATC 1 cut(s) 1879
EcoT22I ATGCAT 1 cut(s) 736
EcoT38I GRGCYC 2 cut(s) 201, 1668
FaeI CATG 6 cut(s) 647, 830, 1128, 1870, 1948, 2246
FalI AAGNNNNNCTT 4 cut(s) 1193, 1225, 1212, 1244
FaqI GGGAC 4 cut(s) 1085, 1307, 1537, 1627
FatI CATG 6 cut(s) 643, 826, 1124, 1866, 1944, 2242
FauI CCCGC 1 cut(s) 1430
FblI GTMKAC 1 cut(s) 1672
Fnu4HI GCNGC 6 cut(s) 120, 350, 965, 1178, 1788, 1807
FokI GGATG 8 cut(s) 632, 743, 871, 1004, 1130, 1337, 2288, 2333
FriOI GRGCYC 2 cut(s) 201, 1668
Fsp4HI GCNGC 6 cut(s) 120, 350, 965, 1178, 1788, 1807
FspBI CTAG 4 cut(s) 267, 276, 929, 2291
GlaI GCGC 2 cut(s) 918, 2295
GluI GCNGC 6 cut(s) 120, 350, 965, 1178, 1788, 1807
GsaI CCCAGC 3 cut(s) 446, 1616, 1714
HaeII RGCGCY 1 cut(s) 2297
HaeIII GGCC 7 cut(s) 119, 285, 349, 1558, 1666, 1709, 2204
HgaI GACGC 2 cut(s) 30, 820
HhaI GCGC 2 cut(s) 919, 2296
Hin1I GRCGYC 1 cut(s) 831
Hin1II CATG 6 cut(s) 647, 830, 1128, 1870, 1948, 2246
Hin6I GCGC 2 cut(s) 917, 2294
HinP1I GCGC 2 cut(s) 917, 2294
HincII GTYRAC 1 cut(s) 1673
HindII GTYRAC 1 cut(s) 1673
HindIII AAGCTT 1 cut(s) 2105
HphI GGTGA 7 cut(s) 118, 599, 806, 982, 1108, 1423, 1921
Hpy166II GTNNAC 6 cut(s) 926, 1198, 1673, 1723, 2177, 2376
Hpy188III TCNNGA 4 cut(s) 289, 1532, 2077, 2357
Hpy8I GTNNAC 6 cut(s) 926, 1198, 1673, 1723, 2177, 2376
HpyAV CCTTC 6 cut(s) 767, 967, 1436, 1588, 1796, 2215
HpyCH4III ACNGT 6 cut(s) 211, 310, 1217, 1699, 2129, 2401
HpyCH4IV ACGT 2 cut(s) 681, 1649
HpyF3I CTNAG 5 cut(s) 114, 238, 296, 1302, 1518
HpySE526I ACGT 2 cut(s) 681, 1649
Hsp92I GRCGYC 1 cut(s) 831
Hsp92II CATG 6 cut(s) 647, 830, 1128, 1870, 1948, 2246
HspAI GCGC 2 cut(s) 917, 2294
Kpn2I TCCGGA 1 cut(s) 1531
KpnI GGTACC 1 cut(s) 2253
LmnI GCTCC 4 cut(s) 155, 204, 1534, 1792
Lsp1109I GCAGC 2 cut(s) 976, 1793
LweI GCATC 6 cut(s) 721, 754, 849, 904, 1349, 2310
MaeI CTAG 4 cut(s) 267, 276, 929, 2291
MaeII ACGT 2 cut(s) 681, 1649
MaeIII GTNAC 3 cut(s) 106, 217, 304
MflI RGATCY 3 cut(s) 880, 1028, 2287
MhlI GDGCHC 6 cut(s) 152, 201, 928, 1668, 1697, 2378
MlyI GAGTC 5 cut(s) 350, 1432, 1510, 1649, 2360
MmeI TCCRAC 2 cut(s) 708, 969
Mph1103I ATGCAT 1 cut(s) 736
MroI TCCGGA 1 cut(s) 1531
MseI TTAA 7 cut(s) 258, 1092, 1376, 2007, 2153, 2282, 2407
MslI CAYNNNNRTG 2 cut(s) 684, 2007
MspA1I CMGCKG 1 cut(s) 1616
MvaI CCWGG 3 cut(s) 474, 1026, 1162
MvnI CGCG 1 cut(s) 919
NciI CCSGG 8 cut(s) 194, 195, 334, 742, 1242, 1555, 1668, 1733
NlaIII CATG 6 cut(s) 647, 830, 1128, 1870, 1948, 2246
NlaIV GGNNCC 7 cut(s) 41, 200, 1073, 1395, 1536, 1666, 2251
NmeAIII GCCGAG 1 cut(s) 1927
NmuCI GTSAC 2 cut(s) 106, 217
NsiI ATGCAT 1 cut(s) 736
NspI RCATGY 2 cut(s) 1870, 2246
PasI CCCWGGG 1 cut(s) 473
PceI AGGCCT 2 cut(s) 285, 2204
PciI ACATGT 1 cut(s) 1866
PcsI WCGNNNNNNNCGW 1 cut(s) 1455
PfeI GAWTC 7 cut(s) 184, 292, 329, 746, 1018, 1102, 2050
PflFI GACNNNGTC 2 cut(s) 1519, 1733
PflMI CCANNNNNTGG 1 cut(s) 442
PfoI TCCNGGA 1 cut(s) 1024
PinAI ACCGGT 1 cut(s) 157
PkrI GCNGC 6 cut(s) 121, 351, 966, 1179, 1789, 1808
PleI GAGTC 5 cut(s) 350, 1432, 1510, 1648, 2360
PpsI GAGTC 5 cut(s) 350, 1432, 1510, 1648, 2360
Ppu21I YACGTR 1 cut(s) 682
PpuMI RGGWCCY 1 cut(s) 1072
PscI ACATGT 1 cut(s) 1866
PshBI ATTAAT 1 cut(s) 2007
PsiI TTATAA 1 cut(s) 806
Psp5II RGGWCCY 1 cut(s) 1072
Psp6I CCWGG 3 cut(s) 472, 1024, 1160
PspFI CCCAGC 3 cut(s) 442, 1612, 1710
PspGI CCWGG 3 cut(s) 472, 1024, 1160
PspN4I GGNNCC 7 cut(s) 41, 200, 1073, 1395, 1536, 1666, 2251
PspOMI GGGCCC 1 cut(s) 1664
PspPPI RGGWCCY 1 cut(s) 1072
PstNI CAGNNNCTG 1 cut(s) 442
PsuI RGATCY 3 cut(s) 880, 1028, 2287
PsyI GACNNNGTC 2 cut(s) 1519, 1733
RsaI GTAC 4 cut(s) 948, 1035, 1214, 2251
RsaNI GTAC 4 cut(s) 947, 1034, 1213, 2250
RseI CAYNNNNRTG 2 cut(s) 684, 2007
Rsr2I CGGWCCG 2 cut(s) 160, 379
RsrII CGGWCCG 2 cut(s) 160, 379
SalI GTCGAC 1 cut(s) 1671
SaqAI TTAA 7 cut(s) 258, 1092, 1376, 2007, 2153, 2282, 2407
SatI GCNGC 6 cut(s) 120, 350, 965, 1178, 1788, 1807
SchI GAGTC 5 cut(s) 350, 1432, 1510, 1649, 2360
SduI GDGCHC 6 cut(s) 152, 201, 928, 1668, 1697, 2378
SfaNI GCATC 6 cut(s) 721, 754, 849, 904, 1349, 2310
SinI GGWCC 8 cut(s) 39, 160, 379, 1072, 1348, 1393, 1534, 1729
SmaI CCCGGG 1 cut(s) 195
SmiMI CAYNNNNRTG 2 cut(s) 684, 2007
SmlI CTYRAG 4 cut(s) 287, 1076, 1280, 1967
SmoI CTYRAG 4 cut(s) 287, 1076, 1280, 1967
SseBI AGGCCT 2 cut(s) 285, 2204
SspMI CTAG 4 cut(s) 267, 276, 929, 2291
StuI AGGCCT 2 cut(s) 285, 2204
TaaI ACNGT 6 cut(s) 211, 310, 1217, 1699, 2129, 2401
TaiI ACGT 2 cut(s) 684, 1652
TaqI TCGA 8 cut(s) 10, 702, 780, 1344, 1581, 1620, 1672, 2053
TatI WGTACW 2 cut(s) 1033, 1212
TauI GCSGC 4 cut(s) 122, 352, 1180, 1790
TfiI GAWTC 7 cut(s) 184, 292, 329, 746, 1018, 1102, 2050
Tru1I TTAA 7 cut(s) 258, 1092, 1376, 2007, 2153, 2282, 2407
Tru9I TTAA 7 cut(s) 258, 1092, 1376, 2007, 2153, 2282, 2407
TscAI CASTG 6 cut(s) 214, 304, 673, 1069, 1222, 1702
TseFI GTSAC 2 cut(s) 106, 217
TseI GCWGC 2 cut(s) 964, 1806
Tsp45I GTSAC 2 cut(s) 106, 217
TspDTI ATGAA 4 cut(s) 426, 660, 2201, 2403
TspGWI ACGGA 4 cut(s) 964, 1461, 1790, 2162
TspMI CCCGGG 1 cut(s) 193
TspRI CASTG 6 cut(s) 214, 304, 673, 1069, 1222, 1702
Tth111I GACNNNGTC 2 cut(s) 1519, 1733
Van91I CCANNNNNTGG 1 cut(s) 442
VneI GTGCAC 2 cut(s) 924, 2374
VpaK11BI GGWCC 8 cut(s) 39, 160, 379, 1072, 1348, 1393, 1534, 1729
VspI ATTAAT 1 cut(s) 2007
XapI RAATTY 4 cut(s) 322, 517, 647, 887
XceI RCATGY 2 cut(s) 1870, 2246
XmaI CCCGGG 1 cut(s) 193
XmiI GTMKAC 1 cut(s) 1672
XspI CTAG 4 cut(s) 267, 276, 929, 2291
Zsp2I ATGCAT 1 cut(s) 736
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.