Rmu_ssc0000410.1_g000044

DTW

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000410.1
OG0012344
Physical Location & Seq
Forward (+)
273719 .. 275038
1320 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000410.1_g000044.1.cds

Sequence Viewer

Length: 1320 bp
atggtgattcctactcaggccatttccaagagacccatttgcccttcctgctccaaacccatccggctctgcctctgcaatcggatccacaacccggggctccacaactctgtaagtgtgaccattctccaacacagctttgagaagaaccaccctcttaattcagctaggattgccagattagggcttaagaatctgaaagtggttactgtttttgatgtacattttgatgcccggttcgctatccggttgctcgacccggatttggaccggaatggttcaaagggtgcatcttttgatggagctgtggaaactggagacacccagaaggtggattctgggttttgtgagtcggaaattgggggttttgaaaattcattaaaacagagaaggtgtagtagtgtggttgattgtaatgagcaatgtagttttggggatgatataggggatatgagtactgctcaaaatcaattaggaagtgtagattctgcagaaaattgtagtgtatcgaacggtgatgcattgcttttagagcaagaagatgaattcattagtgaagcattcactgatggaaagagtgtgcttgctttggagacttgccatatgcatttggatggacaatccaatgtggatgaggcttcaactgctacgactttggattcattatctgatagagattttaattcagttgaaggtgtcaaagttagtaatgctggacctgttataactgctacaattggcaagaatggtgtcattagctctcttactcatacttggatgccacaaacccatcacaagaattcacaatttgatacgatctcagacattacagaagcgcgtgaggctctggcaaagggattcgttgtacagaagttgcaaaagcaggcacctaaaggaaatgttgagttagatgaagctgtagagtttgaagtagaagttccgccggggtctgttctcttgtttccatccaaagaagcagttagtgcgagtgaccttgaagccatggatattaaggtgaagaatttgattgtcttggatggaacatggtcaaaggcaaagagaatgtatgtggaaaacccctggttgaagctattgccacacttgaggttggatttggacaagctgagcttgtacaatgaagtgagagtccagcccaagcccggattcttgtccaccattgagagcattgtgtacgcactaaaggcggtaggggacagtccggaagggctggacaatctcttggatgttttcgagtccatggttggagaccagagacgatgtaaagaagagagattgagcaatgtctcgccgcccggctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DTW

Protein Analysis

439

Amino Acids

47.94

Weight (kDa)

5.04

Isoelectric Point (pI)

34.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012344)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G54880
fragaria_vesca FvH4_3g22471
malus_domestica MD03G1204100.v1.1 MD11G1220900.v1.1
prunus_persica Prupe.4G211300_v2.0.a1
pyrus_communis pycom03g15470 pycom11g19490
rosa_chinensis RchiOBHm_Chr5g0039141
rosa_laevigata RLG00000033897
rosa_multiflora Rmu_sc0000533.1_g000008 Rmu_ssc0000410.1_g000018 Rmu_ssc0000410.1_g000044
rosa_roxburghii Rroxscaffold_1G00040360
rosa_rugosa Rorug05G0177000
rosa_samantha Rh5BG268300 Rh5CG301100 Rh5DG276100
rosa_wichuraiana Rw0G001970 Rw5G024630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 725
AccB1I GGYRCC 1 cut(s) 886
AccB7I CCANNNNNTGG 1 cut(s) 331
AccII CGCG 1 cut(s) 838
AccIII TCCGGA 1 cut(s) 1219
AciI CCGC 3 cut(s) 941, 1205, 1310
AclWI GGATC 2 cut(s) 79, 92
AcsI RAATTY 4 cut(s) 373, 545, 799, 1021
AfaI GTAC 5 cut(s) 222, 457, 867, 1133, 1193
AfiI CCNNNNNNNGG 5 cut(s) 94, 183, 265, 331, 1160
AflII CTTAAG 1 cut(s) 188
AgsI TTSAA 7 cut(s) 282, 371, 642, 692, 929, 998, 1087
AjnI CCWGG 1 cut(s) 1079
AjuI GAANNNNNNNTTGG 2 cut(s) 1148, 1180
AluBI AGCT 8 cut(s) 138, 167, 305, 759, 917, 1090, 1123, 1128
AluI AGCT 8 cut(s) 138, 167, 305, 759, 917, 1090, 1123, 1128
Alw26I GTCTC 6 cut(s) 25, 312, 587, 1260, 1267, 1309
AlwI GGATC 2 cut(s) 79, 92
Ama87I CYCGRG 1 cut(s) 94
Aor13HI TCCGGA 1 cut(s) 1219
AoxI GGCC 1 cut(s) 18
ApoI RAATTY 4 cut(s) 373, 545, 799, 1021
AspLEI GCGC 1 cut(s) 838
AspS9I GGNCC 2 cut(s) 268, 716
AsuC2I CCSGG 7 cut(s) 95, 96, 235, 260, 945, 1161, 1314
AsuHPI GGTGA 3 cut(s) 16, 527, 1027
AvaI CYCGRG 1 cut(s) 94
AvaII GGWCC 2 cut(s) 268, 716
BamHI GGATCC 1 cut(s) 84
BanI GGYRCC 1 cut(s) 886
BanII GRGCYC 1 cut(s) 102
BccI CCATC 7 cut(s) 68, 293, 563, 608, 798, 973, 1031
BciT130I CCWGG 1 cut(s) 1081
BcnI CCSGG 7 cut(s) 95, 96, 235, 260, 945, 1161, 1314
BcoDI GTCTC 6 cut(s) 25, 312, 587, 1260, 1267, 1309
BfaI CTAG 1 cut(s) 168
BfmI CTRYAG 2 cut(s) 489, 918
BfrI CTTAAG 1 cut(s) 188
BisI GCNGC 1 cut(s) 1310
BlpI GCTNAGC 1 cut(s) 1124
BlsI GCNGC 1 cut(s) 1311
BmcAI AGTACT 1 cut(s) 457
Bme1390I CCNGG 8 cut(s) 95, 96, 235, 260, 945, 1081, 1161, 1314
Bme18I GGWCC 2 cut(s) 268, 716
BmeT110I CYCGRG 1 cut(s) 94
BmgT120I GGNCC 2 cut(s) 268, 716
BmiI GGNNCC 3 cut(s) 86, 101, 888
BmrFI CCNGG 8 cut(s) 95, 96, 235, 260, 945, 1081, 1161, 1314
BmsI GCATC 4 cut(s) 220, 299, 508, 768
BplI GAGNNNNNCTC 2 cut(s) 445, 477
BpmI CTGGAG 1 cut(s) 336
Bpu1102I GCTNAGC 1 cut(s) 1124
BpuEI CTTGAG 1 cut(s) 1123
BpuMI CCSGG 7 cut(s) 95, 96, 235, 260, 945, 1161, 1314
BsaI GGTCTC 2 cut(s) 25, 1260
BsaJI CCNNGG 6 cut(s) 94, 95, 944, 1002, 1079, 1257
BsaWI WCCGGW 3 cut(s) 246, 270, 1219
Bsc4I CCNNNNNNNGG 5 cut(s) 94, 183, 265, 331, 1160
Bse1I ACTGG 1 cut(s) 319
Bse3DI GCAATG 3 cut(s) 428, 521, 1306
BseAI TCCGGA 1 cut(s) 1219
BseBI CCWGG 1 cut(s) 1081
BseDI CCNNGG 6 cut(s) 94, 95, 944, 1002, 1079, 1257
BseGI GGATG 8 cut(s) 60, 442, 619, 637, 783, 965, 1042, 1249
BseLI CCNNNNNNNGG 5 cut(s) 94, 183, 265, 331, 1160
BseMI GCAATG 3 cut(s) 428, 521, 1306
BseMII CTCAG 3 cut(s) 29, 834, 1115
BseNI ACTGG 1 cut(s) 319
Bsh1236I CGCG 1 cut(s) 838
BshFI GGCC 1 cut(s) 20
BshNI GGYRCC 1 cut(s) 886
BsiHKCI CYCGRG 1 cut(s) 94
BslFI GGGAC 1 cut(s) 1226
BslI CCNNNNNNNGG 5 cut(s) 94, 183, 265, 331, 1160
BsmAI GTCTC 6 cut(s) 25, 312, 587, 1260, 1267, 1309
BsmBI CGTCTC 1 cut(s) 1267
BsmFI GGGAC 1 cut(s) 1226
BsmI GAATGC 1 cut(s) 560
BsnI GGCC 1 cut(s) 20
Bso31I GGTCTC 2 cut(s) 25, 1260
BsoBI CYCGRG 1 cut(s) 94
Bsp1286I GDGCHC 1 cut(s) 102
Bsp13I TCCGGA 1 cut(s) 1219
Bsp1407I TGTACA 3 cut(s) 220, 865, 1131
Bsp143I GATC 2 cut(s) 84, 816
Bsp1720I GCTNAGC 1 cut(s) 1124
Bsp19I CCATGG 2 cut(s) 1002, 1257
BspACI CCGC 3 cut(s) 941, 1205, 1310
BspANI GGCC 1 cut(s) 20
BspCNI CTCAG 3 cut(s) 28, 833, 1116
BspEI TCCGGA 1 cut(s) 1219
BspFNI CGCG 1 cut(s) 838
BspLI GGNNCC 3 cut(s) 86, 101, 888
BspMAI CTGCAG 1 cut(s) 493
BspPI GGATC 2 cut(s) 79, 92
BspT107I GGYRCC 1 cut(s) 886
BspTI CTTAAG 1 cut(s) 188
BspTNI GGTCTC 2 cut(s) 25, 1260
BsrDI GCAATG 3 cut(s) 428, 521, 1306
BsrGI TGTACA 3 cut(s) 220, 865, 1131
BsrI ACTGG 1 cut(s) 319
BssECI CCNNGG 6 cut(s) 94, 95, 944, 1002, 1079, 1257
BssMI GATC 2 cut(s) 84, 816
BssT1I CCWWGG 2 cut(s) 1002, 1257
Bst2UI CCWGG 1 cut(s) 1081
Bst4CI ACNGT 3 cut(s) 211, 515, 1217
Bst6I CTCTTC 1 cut(s) 1281
BstAFI CTTAAG 1 cut(s) 188
BstAPI GCANNNNNTGC 1 cut(s) 983
BstAUI TGTACA 3 cut(s) 220, 865, 1131
BstC8I GCNNGC 2 cut(s) 585, 885
BstDEI CTNAG 3 cut(s) 15, 820, 1124
BstDSI CCRYGG 2 cut(s) 1002, 1257
BstF5I GGATG 8 cut(s) 60, 442, 619, 637, 783, 965, 1042, 1249
BstFNI CGCG 1 cut(s) 838
BstHHI GCGC 1 cut(s) 838
BstKTI GATC 2 cut(s) 87, 819
BstMAI GTCTC 6 cut(s) 25, 312, 587, 1260, 1267, 1309
BstMBI GATC 2 cut(s) 84, 816
BstMWI GCNNNNNNNGC 8 cut(s) 48, 173, 239, 532, 644, 842, 983, 1202
BstNI CCWGG 1 cut(s) 1081
BstSCI CCNGG 8 cut(s) 93, 94, 233, 258, 943, 1079, 1159, 1312
BstSFI CTRYAG 2 cut(s) 489, 918
BstUI CGCG 1 cut(s) 838
BstX2I RGATCY 1 cut(s) 84
BstYI RGATCY 1 cut(s) 84
BsuRI GGCC 1 cut(s) 20
BtgI CCRYGG 2 cut(s) 1002, 1257
BtsCI GGATG 8 cut(s) 60, 442, 619, 637, 783, 965, 1042, 1249
BtsIMutI CAGTG 1 cut(s) 564
Cac8I GCNNGC 2 cut(s) 585, 885
CfoI GCGC 1 cut(s) 838
Cfr13I GGNCC 2 cut(s) 268, 716
Cfr9I CCCGGG 1 cut(s) 94
Csp6I GTAC 5 cut(s) 221, 456, 866, 1132, 1192
CviAII CATG 3 cut(s) 1003, 1044, 1258
CviQI GTAC 5 cut(s) 221, 456, 866, 1132, 1192
DdeI CTNAG 3 cut(s) 15, 820, 1124
DpnI GATC 2 cut(s) 86, 818
DpnII GATC 2 cut(s) 84, 816
Eam1104I CTCTTC 1 cut(s) 1281
EarI CTCTTC 1 cut(s) 1281
EciI GGCGGA 1 cut(s) 930
Eco130I CCWWGG 2 cut(s) 1002, 1257
Eco24I GRGCYC 1 cut(s) 102
Eco31I GGTCTC 2 cut(s) 25, 1260
Eco47I GGWCC 2 cut(s) 268, 716
Eco88I CYCGRG 1 cut(s) 94
EcoRI GAATTC 2 cut(s) 545, 799
EcoRII CCWGG 1 cut(s) 1079
EcoT14I CCWWGG 2 cut(s) 1002, 1257
EcoT22I ATGCAT 2 cut(s) 523, 609
EcoT38I GRGCYC 1 cut(s) 102
ErhI CCWWGG 2 cut(s) 1002, 1257
Esp3I CGTCTC 1 cut(s) 1267
FaeI CATG 3 cut(s) 1006, 1047, 1261
FalI AAGNNNNNCTT 2 cut(s) 1112, 1144
FaqI GGGAC 1 cut(s) 1226
FatI CATG 3 cut(s) 1002, 1043, 1257
FauNDI CATATG 1 cut(s) 603
Fnu4HI GCNGC 1 cut(s) 1310
FokI GGATG 8 cut(s) 47, 449, 626, 644, 790, 952, 1049, 1256
FriOI GRGCYC 1 cut(s) 102
Fsp4HI GCNGC 1 cut(s) 1310
FspBI CTAG 1 cut(s) 168
GlaI GCGC 1 cut(s) 837
GluI GCNGC 1 cut(s) 1310
GsuI CTGGAG 1 cut(s) 336
HaeIII GGCC 1 cut(s) 20
HhaI GCGC 1 cut(s) 838
Hin1II CATG 3 cut(s) 1006, 1047, 1261
Hin6I GCGC 1 cut(s) 836
HinP1I GCGC 1 cut(s) 836
HphI GGTGA 3 cut(s) 16, 527, 1027
Hpy166II GTNNAC 2 cut(s) 1173, 1192
Hpy188I TCNGA 5 cut(s) 84, 198, 355, 670, 823
Hpy188III TCNNGA 1 cut(s) 1220
Hpy8I GTNNAC 2 cut(s) 1173, 1192
HpyAV CCTTC 5 cut(s) 54, 322, 384, 686, 1217
HpyCH4III ACNGT 3 cut(s) 211, 515, 1217
HpyCH4V TGCA 6 cut(s) 78, 290, 491, 521, 607, 877
HpyF10VI GCNNNNNNNGC 8 cut(s) 48, 173, 239, 532, 644, 842, 983, 1202
HpyF3I CTNAG 3 cut(s) 15, 820, 1124
Hsp92II CATG 3 cut(s) 1006, 1047, 1261
HspAI GCGC 1 cut(s) 836
Kpn2I TCCGGA 1 cut(s) 1219
Kzo9I GATC 2 cut(s) 84, 816
LmnI GCTCC 3 cut(s) 56, 105, 302
LweI GCATC 4 cut(s) 220, 299, 508, 768
MaeI CTAG 1 cut(s) 168
MaeIII GTNAC 3 cut(s) 118, 205, 989
MalI GATC 2 cut(s) 86, 818
MboI GATC 2 cut(s) 84, 816
MboII GAAGA 4 cut(s) 157, 551, 1030, 1298
MfeI CAATTG 1 cut(s) 735
MflI RGATCY 1 cut(s) 84
MhlI GDGCHC 1 cut(s) 102
MlyI GAGTC 3 cut(s) 359, 1155, 1262
MmeI TCCRAC 4 cut(s) 154, 333, 1089, 1243
MnlI CCTC 5 cut(s) 83, 165, 628, 835, 1098
Mph1103I ATGCAT 2 cut(s) 523, 609
MroI TCCGGA 1 cut(s) 1219
MseI TTAA 5 cut(s) 159, 189, 380, 681, 1011
MslI CAYNNNNRTG 2 cut(s) 228, 612
MspCI CTTAAG 1 cut(s) 188
MspR9I CCNGG 8 cut(s) 95, 96, 235, 260, 945, 1081, 1161, 1314
MunI CAATTG 1 cut(s) 735
Mva1269I GAATGC 1 cut(s) 560
MvaI CCWGG 1 cut(s) 1081
MvnI CGCG 1 cut(s) 838
MwoI GCNNNNNNNGC 8 cut(s) 48, 173, 239, 532, 644, 842, 983, 1202
NciI CCSGG 7 cut(s) 95, 96, 235, 260, 945, 1161, 1314
NcoI CCATGG 2 cut(s) 1002, 1257
NdeI CATATG 1 cut(s) 603
NdeII GATC 2 cut(s) 84, 816
NlaIII CATG 3 cut(s) 1006, 1047, 1261
NlaIV GGNNCC 3 cut(s) 86, 101, 888
NmuCI GTSAC 2 cut(s) 118, 989
NsiI ATGCAT 2 cut(s) 523, 609
PctI GAATGC 1 cut(s) 560
PfeI GAWTC 7 cut(s) 7, 193, 335, 485, 659, 858, 1164
PflMI CCANNNNNTGG 1 cut(s) 331
PkrI GCNGC 1 cut(s) 1311
PleI GAGTC 3 cut(s) 358, 1154, 1261
PpsI GAGTC 3 cut(s) 358, 1154, 1261
PsiI TTATAA 1 cut(s) 725
Psp6I CCWGG 1 cut(s) 1079
PspGI CCWGG 1 cut(s) 1079
PspN4I GGNNCC 3 cut(s) 86, 101, 888
PspPI GGNCC 2 cut(s) 268, 716
PstI CTGCAG 1 cut(s) 493
PsuI RGATCY 1 cut(s) 84
RsaI GTAC 5 cut(s) 222, 457, 867, 1133, 1193
RsaNI GTAC 5 cut(s) 221, 456, 866, 1132, 1192
RseI CAYNNNNRTG 2 cut(s) 228, 612
SaqAI TTAA 5 cut(s) 159, 189, 380, 681, 1011
SatI GCNGC 1 cut(s) 1310
Sau3AI GATC 2 cut(s) 84, 816
Sau96I GGNCC 2 cut(s) 268, 716
ScaI AGTACT 1 cut(s) 457
SchI GAGTC 3 cut(s) 359, 1155, 1262
ScrFI CCNGG 8 cut(s) 95, 96, 235, 260, 945, 1081, 1161, 1314
SduI GDGCHC 1 cut(s) 102
SfaNI GCATC 4 cut(s) 220, 299, 508, 768
SfcI CTRYAG 2 cut(s) 489, 918
SinI GGWCC 2 cut(s) 268, 716
SmaI CCCGGG 1 cut(s) 96
SmiMI CAYNNNNRTG 2 cut(s) 228, 612
SmlI CTYRAG 2 cut(s) 188, 1102
SmoI CTYRAG 2 cut(s) 188, 1102
SsiI CCGC 3 cut(s) 941, 1205, 1310
SspMI CTAG 1 cut(s) 168
StyD4I CCNGG 8 cut(s) 93, 94, 233, 258, 943, 1079, 1159, 1312
StyI CCWWGG 2 cut(s) 1002, 1257
TaaI ACNGT 3 cut(s) 211, 515, 1217
TaqI TCGA 3 cut(s) 255, 509, 1251
TatI WGTACW 4 cut(s) 220, 455, 865, 1131
TauI GCSGC 1 cut(s) 1312
TfiI GAWTC 7 cut(s) 7, 193, 335, 485, 659, 858, 1164
Tru1I TTAA 5 cut(s) 159, 189, 380, 681, 1011
Tru9I TTAA 5 cut(s) 159, 189, 380, 681, 1011
TscAI CASTG 1 cut(s) 571
TseFI GTSAC 2 cut(s) 118, 989
Tsp45I GTSAC 2 cut(s) 118, 989
TspDTI ATGAA 6 cut(s) 366, 538, 558, 651, 927, 1152
TspMI CCCGGG 1 cut(s) 94
TspRI CASTG 1 cut(s) 571
Van91I CCANNNNNTGG 1 cut(s) 331
Vha464I CTTAAG 1 cut(s) 188
VpaK11BI GGWCC 2 cut(s) 268, 716
XapI RAATTY 4 cut(s) 373, 545, 799, 1021
XmaI CCCGGG 1 cut(s) 94
XspI CTAG 1 cut(s) 168
ZrmI AGTACT 1 cut(s) 457
Zsp2I ATGCAT 2 cut(s) 523, 609
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.