Rmu_sc0000533.1_g000008

DTW

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000533.1
OG0012344
Physical Location & Seq
Reverse (-)
31288 .. 32619
1332 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000533.1_g000008.1.cds

Sequence Viewer

Length: 1332 bp
atggtgattcctactcaggccatttccaagagacccatttgcccttcctgctccaaacccatccggctctgcctctgcaatcggatccacaacccggggctccacaactctgtaagtgtgaccattctccaacacagctttgagaagaaccaccctcttaattcagctaggattgccagattagggcttaagaatctgaaagtggttactgtttttgatgtacattttgatgcccggttcgctatccggttgctcgacccggatttggaaacgggtttggaccggaatggttcaaagggtgcatcttttgatggagctgtggcaactggagagacccagaaggtggattctgggttttgtgagtcggaaattgggggttttgaaaattcatcaaaacagagaaggtgtagtagtgtggttgattgtaatgagcaatgtagttttggggatgatataggggatatgagtagtgctcaaaatcaattagggagtgtagattctgcagaaaattgtagtgtatcgaacggtgatgcattgcttgtagagcaagaagatgaattcattagtgaagcattcactgatggaaagagtgtgcttgctttggagacttgccctatgcatttggatggacaatccaatgtggatgaggcttcaactgctacgactttggattcattatctgatagagattttaattcagttgaaggtgtcaaagttagtaatgctggacctgttataactgctacaattggcaagaatggtgtcattagctctcttactcatacttggatgccacaaacccatcacaagaattcacaatttgatacgatctcagacattacagaagcgcgtgaggctctggcaaagggattcgttgtacagaagttgcaaaagcaggcacctaaaggaaatgttgagttagatgaagctgtagagtttgaagtagaagttcctccggggtctgttctcttgtttccatccaaagaagcagttagtgccagtgaccttgaagccatggatattaaggtgaagaatttgattgtcttggatggaacatggtcaaaggcaaagagaatgtatgtggaaaacccctggttgaagctattgccacacttgaggttggatttggacaagcttagcttgtataatgaagtgagagtccagcccaagcctggattcttgtccaccattgagagcattgtatacgcactaaaagcggtaggggacagtcccgaagggctggacaatctcttggatgttttcgagtccatggttggagaccagagacgatgtaaagaagagagattgagcaatgtctcgccgcgcggctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DTW

Protein Analysis

443

Amino Acids

48.26

Weight (kDa)

5.06

Isoelectric Point (pI)

33.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012344)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G54880
fragaria_vesca FvH4_3g22471
malus_domestica MD03G1204100.v1.1 MD11G1220900.v1.1
prunus_persica Prupe.4G211300_v2.0.a1
pyrus_communis pycom03g15470 pycom11g19490
rosa_chinensis RchiOBHm_Chr5g0039141
rosa_laevigata RLG00000033897
rosa_multiflora Rmu_sc0000533.1_g000008 Rmu_ssc0000410.1_g000018 Rmu_ssc0000410.1_g000044
rosa_roxburghii Rroxscaffold_1G00040360
rosa_rugosa Rorug05G0177000
rosa_samantha Rh5BG268300 Rh5CG301100 Rh5DG276100
rosa_wichuraiana Rw0G001970 Rw5G024630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 737
AccB1I GGYRCC 1 cut(s) 898
AccB7I CCANNNNNTGG 1 cut(s) 343
AccI GTMKAC 1 cut(s) 1203
AccII CGCG 3 cut(s) 850, 1324, 1326
AciI CCGC 3 cut(s) 1217, 1322, 1326
AclWI GGATC 2 cut(s) 79, 92
AcsI RAATTY 4 cut(s) 385, 557, 811, 1033
AfaI GTAC 2 cut(s) 222, 879
AfiI CCNNNNNNNGG 5 cut(s) 94, 183, 265, 343, 1172
AflII CTTAAG 1 cut(s) 188
AgsI TTSAA 7 cut(s) 294, 383, 654, 704, 941, 1010, 1099
AjnI CCWGG 2 cut(s) 1091, 1171
AjuI GAANNNNNNNTTGG 2 cut(s) 1160, 1192
AluBI AGCT 8 cut(s) 138, 167, 317, 771, 929, 1102, 1135, 1140
AluI AGCT 8 cut(s) 138, 167, 317, 771, 929, 1102, 1135, 1140
Alw21I GWGCWC 1 cut(s) 475
Alw26I GTCTC 6 cut(s) 25, 326, 599, 1272, 1279, 1321
AlwI GGATC 2 cut(s) 79, 92
Ama87I CYCGRG 1 cut(s) 94
AoxI GGCC 1 cut(s) 18
ApoI RAATTY 4 cut(s) 385, 557, 811, 1033
AspLEI GCGC 2 cut(s) 850, 1326
AspS9I GGNCC 2 cut(s) 280, 728
AsuC2I CCSGG 5 cut(s) 95, 96, 235, 260, 957
AsuHPI GGTGA 3 cut(s) 16, 539, 1039
AvaI CYCGRG 1 cut(s) 94
AvaII GGWCC 2 cut(s) 280, 728
BamHI GGATCC 1 cut(s) 84
BanI GGYRCC 1 cut(s) 898
BanII GRGCYC 1 cut(s) 102
Bbv12I GWGCWC 1 cut(s) 475
BccI CCATC 7 cut(s) 68, 305, 575, 620, 810, 985, 1043
BciT130I CCWGG 2 cut(s) 1093, 1173
BcnI CCSGG 5 cut(s) 95, 96, 235, 260, 957
BcoDI GTCTC 6 cut(s) 25, 326, 599, 1272, 1279, 1321
BfaI CTAG 1 cut(s) 168
BfmI CTRYAG 2 cut(s) 501, 930
BfrI CTTAAG 1 cut(s) 188
BisI GCNGC 2 cut(s) 1322, 1327
BlpI GCTNAGC 1 cut(s) 1136
BlsI GCNGC 2 cut(s) 1323, 1328
Bme1390I CCNGG 7 cut(s) 95, 96, 235, 260, 957, 1093, 1173
Bme18I GGWCC 2 cut(s) 280, 728
BmeT110I CYCGRG 1 cut(s) 94
BmgT120I GGNCC 2 cut(s) 280, 728
BmiI GGNNCC 3 cut(s) 86, 101, 900
BmrFI CCNGG 7 cut(s) 95, 96, 235, 260, 957, 1093, 1173
BmsI GCATC 4 cut(s) 220, 311, 520, 780
BplI GAGNNNNNCTC 2 cut(s) 457, 489
BpmI CTGGAG 1 cut(s) 348
Bpu1102I GCTNAGC 1 cut(s) 1136
BpuEI CTTGAG 1 cut(s) 1135
BpuMI CCSGG 5 cut(s) 95, 96, 235, 260, 957
BsaI GGTCTC 3 cut(s) 25, 326, 1272
BsaJI CCNNGG 6 cut(s) 94, 95, 956, 1014, 1091, 1269
BsaWI WCCGGW 2 cut(s) 246, 282
Bsc4I CCNNNNNNNGG 5 cut(s) 94, 183, 265, 343, 1172
Bse1I ACTGG 2 cut(s) 331, 999
Bse3DI GCAATG 3 cut(s) 440, 533, 1318
BseBI CCWGG 2 cut(s) 1093, 1173
BseDI CCNNGG 6 cut(s) 94, 95, 956, 1014, 1091, 1269
BseGI GGATG 8 cut(s) 60, 454, 631, 649, 795, 977, 1054, 1261
BseLI CCNNNNNNNGG 5 cut(s) 94, 183, 265, 343, 1172
BseMI GCAATG 3 cut(s) 440, 533, 1318
BseMII CTCAG 2 cut(s) 29, 846
BseNI ACTGG 2 cut(s) 331, 999
Bsh1236I CGCG 3 cut(s) 850, 1324, 1326
BshFI GGCC 1 cut(s) 20
BshNI GGYRCC 1 cut(s) 898
BsiHKAI GWGCWC 1 cut(s) 475
BsiHKCI CYCGRG 1 cut(s) 94
BsiSI CCGG 7 cut(s) 64, 95, 235, 247, 260, 283, 956
BslFI GGGAC 2 cut(s) 1215, 1238
BslI CCNNNNNNNGG 5 cut(s) 94, 183, 265, 343, 1172
BsmAI GTCTC 6 cut(s) 25, 326, 599, 1272, 1279, 1321
BsmBI CGTCTC 1 cut(s) 1279
BsmFI GGGAC 2 cut(s) 1215, 1238
BsmI GAATGC 1 cut(s) 572
BsnI GGCC 1 cut(s) 20
Bso31I GGTCTC 3 cut(s) 25, 326, 1272
BsoBI CYCGRG 1 cut(s) 94
Bsp1286I GDGCHC 2 cut(s) 102, 475
Bsp1407I TGTACA 2 cut(s) 220, 877
Bsp143I GATC 2 cut(s) 84, 828
Bsp1720I GCTNAGC 1 cut(s) 1136
Bsp19I CCATGG 2 cut(s) 1014, 1269
BspACI CCGC 3 cut(s) 1217, 1322, 1326
BspANI GGCC 1 cut(s) 20
BspCNI CTCAG 2 cut(s) 28, 845
BspFNI CGCG 3 cut(s) 850, 1324, 1326
BspLI GGNNCC 3 cut(s) 86, 101, 900
BspMAI CTGCAG 1 cut(s) 505
BspPI GGATC 2 cut(s) 79, 92
BspT107I GGYRCC 1 cut(s) 898
BspTI CTTAAG 1 cut(s) 188
BspTNI GGTCTC 3 cut(s) 25, 326, 1272
BsrDI GCAATG 3 cut(s) 440, 533, 1318
BsrGI TGTACA 2 cut(s) 220, 877
BsrI ACTGG 2 cut(s) 331, 999
BssECI CCNNGG 6 cut(s) 94, 95, 956, 1014, 1091, 1269
BssMI GATC 2 cut(s) 84, 828
BssNAI GTATAC 1 cut(s) 1204
BssT1I CCWWGG 2 cut(s) 1014, 1269
Bst1107I GTATAC 1 cut(s) 1204
Bst2UI CCWGG 2 cut(s) 1093, 1173
Bst4CI ACNGT 3 cut(s) 211, 527, 1229
Bst6I CTCTTC 1 cut(s) 1293
BstAFI CTTAAG 1 cut(s) 188
BstAPI GCANNNNNTGC 1 cut(s) 995
BstAUI TGTACA 2 cut(s) 220, 877
BstC8I GCNNGC 2 cut(s) 597, 897
BstDEI CTNAG 3 cut(s) 15, 832, 1136
BstDSI CCRYGG 2 cut(s) 1014, 1269
BstF5I GGATG 8 cut(s) 60, 454, 631, 649, 795, 977, 1054, 1261
BstFNI CGCG 3 cut(s) 850, 1324, 1326
BstHHI GCGC 2 cut(s) 850, 1326
BstKTI GATC 2 cut(s) 87, 831
BstMAI GTCTC 6 cut(s) 25, 326, 599, 1272, 1279, 1321
BstMBI GATC 2 cut(s) 84, 828
BstMWI GCNNNNNNNGC 8 cut(s) 48, 173, 239, 544, 656, 854, 995, 1214
BstNI CCWGG 2 cut(s) 1093, 1173
BstSCI CCNGG 7 cut(s) 93, 94, 233, 258, 955, 1091, 1171
BstSFI CTRYAG 2 cut(s) 501, 930
BstUI CGCG 3 cut(s) 850, 1324, 1326
BstX2I RGATCY 1 cut(s) 84
BstYI RGATCY 1 cut(s) 84
BstZ17I GTATAC 1 cut(s) 1204
BsuRI GGCC 1 cut(s) 20
BtgI CCRYGG 2 cut(s) 1014, 1269
BtsCI GGATG 8 cut(s) 60, 454, 631, 649, 795, 977, 1054, 1261
BtsIMutI CAGTG 2 cut(s) 576, 1006
Cac8I GCNNGC 2 cut(s) 597, 897
CfoI GCGC 2 cut(s) 850, 1326
Cfr13I GGNCC 2 cut(s) 280, 728
Cfr9I CCCGGG 1 cut(s) 94
Csp6I GTAC 2 cut(s) 221, 878
CviAII CATG 3 cut(s) 1015, 1056, 1270
CviQI GTAC 2 cut(s) 221, 878
DdeI CTNAG 3 cut(s) 15, 832, 1136
DpnI GATC 2 cut(s) 86, 830
DpnII GATC 2 cut(s) 84, 828
Eam1104I CTCTTC 1 cut(s) 1293
EarI CTCTTC 1 cut(s) 1293
Eco130I CCWWGG 2 cut(s) 1014, 1269
Eco24I GRGCYC 1 cut(s) 102
Eco31I GGTCTC 3 cut(s) 25, 326, 1272
Eco47I GGWCC 2 cut(s) 280, 728
Eco88I CYCGRG 1 cut(s) 94
EcoRI GAATTC 2 cut(s) 557, 811
EcoRII CCWGG 2 cut(s) 1091, 1171
EcoT14I CCWWGG 2 cut(s) 1014, 1269
EcoT22I ATGCAT 2 cut(s) 535, 621
EcoT38I GRGCYC 1 cut(s) 102
ErhI CCWWGG 2 cut(s) 1014, 1269
Esp3I CGTCTC 1 cut(s) 1279
FaeI CATG 3 cut(s) 1018, 1059, 1273
FalI AAGNNNNNCTT 2 cut(s) 1124, 1156
FaqI GGGAC 2 cut(s) 1215, 1238
FatI CATG 3 cut(s) 1014, 1055, 1269
FblI GTMKAC 1 cut(s) 1203
Fnu4HI GCNGC 2 cut(s) 1322, 1327
FokI GGATG 8 cut(s) 47, 461, 638, 656, 802, 964, 1061, 1268
FriOI GRGCYC 1 cut(s) 102
Fsp4HI GCNGC 2 cut(s) 1322, 1327
FspBI CTAG 1 cut(s) 168
GlaI GCGC 2 cut(s) 849, 1325
GluI GCNGC 2 cut(s) 1322, 1327
GsuI CTGGAG 1 cut(s) 348
HaeIII GGCC 1 cut(s) 20
HapII CCGG 7 cut(s) 64, 95, 235, 247, 260, 283, 956
HhaI GCGC 2 cut(s) 850, 1326
Hin1II CATG 3 cut(s) 1018, 1059, 1273
Hin6I GCGC 2 cut(s) 848, 1324
HinP1I GCGC 2 cut(s) 848, 1324
HindIII AAGCTT 1 cut(s) 1133
HpaII CCGG 7 cut(s) 64, 95, 235, 247, 260, 283, 956
HphI GGTGA 3 cut(s) 16, 539, 1039
Hpy166II GTNNAC 2 cut(s) 1185, 1204
Hpy188I TCNGA 5 cut(s) 84, 198, 367, 682, 835
Hpy188III TCNNGA 1 cut(s) 1232
Hpy8I GTNNAC 2 cut(s) 1185, 1204
HpyAV CCTTC 5 cut(s) 54, 334, 396, 698, 1229
HpyCH4III ACNGT 3 cut(s) 211, 527, 1229
HpyCH4V TGCA 6 cut(s) 78, 302, 503, 533, 619, 889
HpyF10VI GCNNNNNNNGC 8 cut(s) 48, 173, 239, 544, 656, 854, 995, 1214
HpyF3I CTNAG 3 cut(s) 15, 832, 1136
Hsp92II CATG 3 cut(s) 1018, 1059, 1273
HspAI GCGC 2 cut(s) 848, 1324
Kzo9I GATC 2 cut(s) 84, 828
LmnI GCTCC 3 cut(s) 56, 105, 314
LweI GCATC 4 cut(s) 220, 311, 520, 780
MaeI CTAG 1 cut(s) 168
MaeIII GTNAC 3 cut(s) 118, 205, 1001
MalI GATC 2 cut(s) 86, 830
MboI GATC 2 cut(s) 84, 828
MboII GAAGA 4 cut(s) 157, 563, 1042, 1310
MfeI CAATTG 1 cut(s) 747
MflI RGATCY 1 cut(s) 84
MhlI GDGCHC 2 cut(s) 102, 475
MlyI GAGTC 3 cut(s) 371, 1167, 1274
MmeI TCCRAC 4 cut(s) 154, 345, 1101, 1255
MnlI CCTC 6 cut(s) 83, 165, 640, 847, 963, 1110
Mph1103I ATGCAT 2 cut(s) 535, 621
MseI TTAA 4 cut(s) 159, 189, 693, 1023
MslI CAYNNNNRTG 2 cut(s) 228, 624
MspCI CTTAAG 1 cut(s) 188
MspI CCGG 7 cut(s) 64, 95, 235, 247, 260, 283, 956
MspR9I CCNGG 7 cut(s) 95, 96, 235, 260, 957, 1093, 1173
MunI CAATTG 1 cut(s) 747
Mva1269I GAATGC 1 cut(s) 572
MvaI CCWGG 2 cut(s) 1093, 1173
MvnI CGCG 3 cut(s) 850, 1324, 1326
MwoI GCNNNNNNNGC 8 cut(s) 48, 173, 239, 544, 656, 854, 995, 1214
NciI CCSGG 5 cut(s) 95, 96, 235, 260, 957
NcoI CCATGG 2 cut(s) 1014, 1269
NdeII GATC 2 cut(s) 84, 828
NlaIII CATG 3 cut(s) 1018, 1059, 1273
NlaIV GGNNCC 3 cut(s) 86, 101, 900
NmuCI GTSAC 2 cut(s) 118, 1001
NsiI ATGCAT 2 cut(s) 535, 621
PctI GAATGC 1 cut(s) 572
PfeI GAWTC 7 cut(s) 7, 193, 347, 497, 671, 870, 1176
PflMI CCANNNNNTGG 1 cut(s) 343
PkrI GCNGC 2 cut(s) 1323, 1328
PleI GAGTC 3 cut(s) 370, 1166, 1273
PpsI GAGTC 3 cut(s) 370, 1166, 1273
PsiI TTATAA 1 cut(s) 737
Psp6I CCWGG 2 cut(s) 1091, 1171
PspGI CCWGG 2 cut(s) 1091, 1171
PspN4I GGNNCC 3 cut(s) 86, 101, 900
PspPI GGNCC 2 cut(s) 280, 728
PstI CTGCAG 1 cut(s) 505
PsuI RGATCY 1 cut(s) 84
RsaI GTAC 2 cut(s) 222, 879
RsaNI GTAC 2 cut(s) 221, 878
RseI CAYNNNNRTG 2 cut(s) 228, 624
SaqAI TTAA 4 cut(s) 159, 189, 693, 1023
SatI GCNGC 2 cut(s) 1322, 1327
Sau3AI GATC 2 cut(s) 84, 828
Sau96I GGNCC 2 cut(s) 280, 728
SchI GAGTC 3 cut(s) 371, 1167, 1274
ScrFI CCNGG 7 cut(s) 95, 96, 235, 260, 957, 1093, 1173
SduI GDGCHC 2 cut(s) 102, 475
SfaNI GCATC 4 cut(s) 220, 311, 520, 780
SfcI CTRYAG 2 cut(s) 501, 930
SinI GGWCC 2 cut(s) 280, 728
SmaI CCCGGG 1 cut(s) 96
SmiMI CAYNNNNRTG 2 cut(s) 228, 624
SmlI CTYRAG 2 cut(s) 188, 1114
SmoI CTYRAG 2 cut(s) 188, 1114
SsiI CCGC 3 cut(s) 1217, 1322, 1326
SspMI CTAG 1 cut(s) 168
StyD4I CCNGG 7 cut(s) 93, 94, 233, 258, 955, 1091, 1171
StyI CCWWGG 2 cut(s) 1014, 1269
TaaI ACNGT 3 cut(s) 211, 527, 1229
TaqI TCGA 3 cut(s) 255, 521, 1263
TatI WGTACW 2 cut(s) 220, 877
TauI GCSGC 2 cut(s) 1324, 1329
TfiI GAWTC 7 cut(s) 7, 193, 347, 497, 671, 870, 1176
Tru1I TTAA 4 cut(s) 159, 189, 693, 1023
Tru9I TTAA 4 cut(s) 159, 189, 693, 1023
TscAI CASTG 2 cut(s) 583, 1006
TseFI GTSAC 2 cut(s) 118, 1001
Tsp45I GTSAC 2 cut(s) 118, 1001
TspDTI ATGAA 6 cut(s) 378, 550, 570, 663, 939, 1164
TspMI CCCGGG 1 cut(s) 94
TspRI CASTG 2 cut(s) 583, 1006
Van91I CCANNNNNTGG 1 cut(s) 343
Vha464I CTTAAG 1 cut(s) 188
VpaK11BI GGWCC 2 cut(s) 280, 728
XapI RAATTY 4 cut(s) 385, 557, 811, 1033
XcmI CCANNNNNNNNNTGG 1 cut(s) 1169
XmaI CCCGGG 1 cut(s) 94
XmiI GTMKAC 1 cut(s) 1203
XspI CTAG 1 cut(s) 168
Zsp2I ATGCAT 2 cut(s) 535, 621
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.