Rh5BG268300

DTW

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0012344
Physical Location & Seq
Forward (+)
33487991 .. 33489409
1419 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG268300.1

Sequence Viewer

Length: 1419 bp
ATGTTGGTTCGGTTCGTGAGTCCGATCCACTCACAGACCCGAAACAAGCGTGTCTGCCCAATTCTCCATAAACCCTACTTTCTTCCCCAAACCCTAAAAATGGTGATTCCTACTCAGGCCATTTCCAAGAGACCCATTTGCCCTTCCTGCTCCAAACCCATCCGGCTCTGCCTCTGCAATCGGATCCACAACCCGGGGCTCCACAACTCTGTAAGTGTGACCATTCTCCAACACAGCTTTGAGAAGAACCACCCTCTTAATTCAGCTAGGATTGCCAGATTAGGGCTTAAGAATCTGAAAGTGGTTACTGTTTTTGATGTACATTTTGATGCCCGGTTCGCTATCCGGTTGCTCGACCCGGATTTGGACCGGAATGGTTCAAAGGGTGCATCTTTTGATGGCGCTGTGGAAACTGGAGAGACCCAGAAGGTGGATTCTGGGTTTTGTGAGTCGGAAATTGGGGGTTTTGAAAATTCATTAAAACAGAGAAGGTGTAGTAGTGTGGTTGATTGTAATGAGCAATGTAGTTTTGGGGATGATATAGGGGATATGAGTACTGCTCAAAATCAATTAGGAAGTGTAGATTCTGCAGAAAATTGTAGTGTATCGAACGGTGATGCATTGCTTTTAGAGCAAGAAGATGAATTCATTAGTGAAGCATTCACTGATGGAAAGAGTGTGCTTGCTTTGGAGACTTGCCATATGCATTTGGATGGACAATCCAATGTGGATGAGGCTTCAACTGCTACGACTTTGGATTCATTATCTGATAGAGATTTTAATTCAGTTGAAGGTGTCAAAGTTAGTAATGCTGGACCTGTTATAACTGCTACAATTGGCAAGAATGGTGTCATTAGCTCTCTTACTCATACTTGGATGCCACAAACCCATCACAAGAATTCACAATTTGATACGATCTCAGACATTACAGAAGCACGTGAGGCTCTGGCAAAGGGATTCGTTGTACAGAAGTTGCAAAAGCAGGCACCTAAAGGAAATGTTGAGTTAGATGAAGCTGTAGAGTTTGAAGTAGAAGTTCCTCCGGGGTCTGTTCTCTTGTTTCCATCCAAAGAAGCAGTTAGTGCAAGTGACCTTGAAGCCATGGATATTAAGGTGAAGAATTTGATTGTCTTGGATGGAACATGGTCAAAGGCAAAGAGAATGTATGTGGAAAACCCCTGGTTGAAGCTATTGCCACACTTGAGGTTGGATTTGGACAAGCTTAGCTTGTATAATGAAGTGAGAGTCCAGCCCAAGCCGGGATTCTTGTCCACCATTGAGAGCATTGTGTACGCACTAAAGGCGGTAGGGGACAGTCCGGAAGGGCTGGACAATCTCTTGGATGTTTTCGAGTCCATGGTTGGAGACCAGAGACGATGTAAAGAAGAGAGATTGAGCAATGTCTCGCCGCCCGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DTW

Protein Analysis

472

Amino Acids

51.89

Weight (kDa)

5.53

Isoelectric Point (pI)

38.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DTW PF03942 44 - 105 7.4e-09 DTW domain
DTW PF03942 291 - 457 6.7e-28 DTW domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012344)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G54880
fragaria_vesca FvH4_3g22471
malus_domestica MD03G1204100.v1.1 MD11G1220900.v1.1
prunus_persica Prupe.4G211300_v2.0.a1
pyrus_communis pycom03g15470 pycom11g19490
rosa_chinensis RchiOBHm_Chr5g0039141
rosa_laevigata RLG00000033897
rosa_multiflora Rmu_sc0000533.1_g000008 Rmu_ssc0000410.1_g000018 Rmu_ssc0000410.1_g000044
rosa_roxburghii Rroxscaffold_1G00040360
rosa_rugosa Rorug05G0177000
rosa_samantha Rh5BG268300 Rh5CG301100 Rh5DG276100
rosa_wichuraiana Rw0G001970 Rw5G024630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 824
AccB1I GGYRCC 1 cut(s) 985
AccB7I CCANNNNNTGG 1 cut(s) 430
AccIII TCCGGA 1 cut(s) 1318
AciI CCGC 2 cut(s) 1304, 1409
AclWI GGATC 3 cut(s) 19, 178, 191
AcsI RAATTY 4 cut(s) 472, 644, 898, 1120
AcvI CACGTG 1 cut(s) 938
AfaI GTAC 4 cut(s) 321, 556, 966, 1292
AfiI CCNNNNNNNGG 6 cut(s) 100, 193, 282, 364, 430, 1259
AflII CTTAAG 1 cut(s) 287
AgsI TTSAA 7 cut(s) 381, 470, 741, 791, 1028, 1097, 1186
AjnI CCWGG 1 cut(s) 1178
AjuI GAANNNNNNNTTGG 2 cut(s) 1247, 1279
AluBI AGCT 7 cut(s) 237, 266, 858, 1016, 1189, 1222, 1227
AluI AGCT 7 cut(s) 237, 266, 858, 1016, 1189, 1222, 1227
Alw26I GTCTC 6 cut(s) 124, 413, 686, 1359, 1366, 1408
AlwI GGATC 3 cut(s) 19, 178, 191
Ama87I CYCGRG 1 cut(s) 193
Aor13HI TCCGGA 1 cut(s) 1318
AoxI GGCC 1 cut(s) 117
ApoI RAATTY 4 cut(s) 472, 644, 898, 1120
AspLEI GCGC 1 cut(s) 404
AspS9I GGNCC 2 cut(s) 367, 815
AsuC2I CCSGG 7 cut(s) 194, 195, 334, 359, 1044, 1260, 1413
AsuHPI GGTGA 3 cut(s) 115, 626, 1126
AvaI CYCGRG 1 cut(s) 193
AvaII GGWCC 2 cut(s) 367, 815
BamHI GGATCC 1 cut(s) 183
BanI GGYRCC 1 cut(s) 985
BanII GRGCYC 1 cut(s) 201
BbrPI CACGTG 1 cut(s) 938
BccI CCATC 7 cut(s) 167, 392, 662, 707, 897, 1072, 1130
BciT130I CCWGG 1 cut(s) 1180
BcnI CCSGG 7 cut(s) 194, 195, 334, 359, 1044, 1260, 1413
BcoDI GTCTC 6 cut(s) 124, 413, 686, 1359, 1366, 1408
BfaI CTAG 1 cut(s) 267
BfmI CTRYAG 2 cut(s) 588, 1017
BfoI RGCGCY 1 cut(s) 405
BfrI CTTAAG 1 cut(s) 287
BisI GCNGC 1 cut(s) 1409
BlpI GCTNAGC 1 cut(s) 1223
BlsI GCNGC 1 cut(s) 1410
BmcAI AGTACT 1 cut(s) 556
Bme1390I CCNGG 8 cut(s) 194, 195, 334, 359, 1044, 1180, 1260, 1413
Bme18I GGWCC 2 cut(s) 367, 815
BmeT110I CYCGRG 1 cut(s) 193
BmgT120I GGNCC 2 cut(s) 367, 815
BmiI GGNNCC 3 cut(s) 185, 200, 987
BmrFI CCNGG 8 cut(s) 194, 195, 334, 359, 1044, 1180, 1260, 1413
BmsI GCATC 4 cut(s) 319, 398, 607, 867
BplI GAGNNNNNCTC 2 cut(s) 544, 576
BpmI CTGGAG 1 cut(s) 435
Bpu1102I GCTNAGC 1 cut(s) 1223
BpuEI CTTGAG 1 cut(s) 1222
BpuMI CCSGG 7 cut(s) 194, 195, 334, 359, 1044, 1260, 1413
BsaAI YACGTR 1 cut(s) 938
BsaI GGTCTC 3 cut(s) 124, 413, 1359
BsaJI CCNNGG 6 cut(s) 193, 194, 1043, 1101, 1178, 1356
BsaWI WCCGGW 3 cut(s) 345, 369, 1318
Bsc4I CCNNNNNNNGG 6 cut(s) 100, 193, 282, 364, 430, 1259
Bse1I ACTGG 1 cut(s) 418
Bse3DI GCAATG 3 cut(s) 527, 620, 1405
BseAI TCCGGA 1 cut(s) 1318
BseBI CCWGG 1 cut(s) 1180
BseDI CCNNGG 6 cut(s) 193, 194, 1043, 1101, 1178, 1356
BseGI GGATG 8 cut(s) 159, 541, 718, 736, 882, 1064, 1141, 1348
BseLI CCNNNNNNNGG 6 cut(s) 100, 193, 282, 364, 430, 1259
BseMI GCAATG 3 cut(s) 527, 620, 1405
BseMII CTCAG 2 cut(s) 128, 933
BseNI ACTGG 1 cut(s) 418
BshFI GGCC 1 cut(s) 119
BshNI GGYRCC 1 cut(s) 985
BsiHKCI CYCGRG 1 cut(s) 193
BslFI GGGAC 1 cut(s) 1325
BslI CCNNNNNNNGG 6 cut(s) 100, 193, 282, 364, 430, 1259
BsmAI GTCTC 6 cut(s) 124, 413, 686, 1359, 1366, 1408
BsmBI CGTCTC 1 cut(s) 1366
BsmFI GGGAC 1 cut(s) 1325
BsmI GAATGC 1 cut(s) 659
BsnI GGCC 1 cut(s) 119
Bso31I GGTCTC 3 cut(s) 124, 413, 1359
BsoBI CYCGRG 1 cut(s) 193
Bsp1286I GDGCHC 1 cut(s) 201
Bsp13I TCCGGA 1 cut(s) 1318
Bsp1407I TGTACA 2 cut(s) 319, 964
Bsp143I GATC 3 cut(s) 24, 183, 915
Bsp1720I GCTNAGC 1 cut(s) 1223
Bsp19I CCATGG 2 cut(s) 1101, 1356
BspACI CCGC 2 cut(s) 1304, 1409
BspANI GGCC 1 cut(s) 119
BspCNI CTCAG 2 cut(s) 127, 932
BspEI TCCGGA 1 cut(s) 1318
BspLI GGNNCC 3 cut(s) 185, 200, 987
BspMAI CTGCAG 1 cut(s) 592
BspPI GGATC 3 cut(s) 19, 178, 191
BspT107I GGYRCC 1 cut(s) 985
BspTI CTTAAG 1 cut(s) 287
BspTNI GGTCTC 3 cut(s) 124, 413, 1359
BsrDI GCAATG 3 cut(s) 527, 620, 1405
BsrGI TGTACA 2 cut(s) 319, 964
BsrI ACTGG 1 cut(s) 418
BssECI CCNNGG 6 cut(s) 193, 194, 1043, 1101, 1178, 1356
BssMI GATC 3 cut(s) 24, 183, 915
BssT1I CCWWGG 2 cut(s) 1101, 1356
Bst2UI CCWGG 1 cut(s) 1180
Bst4CI ACNGT 3 cut(s) 310, 614, 1316
Bst6I CTCTTC 1 cut(s) 1380
BstAFI CTTAAG 1 cut(s) 287
BstAPI GCANNNNNTGC 1 cut(s) 1082
BstAUI TGTACA 2 cut(s) 319, 964
BstBAI YACGTR 1 cut(s) 938
BstC8I GCNNGC 2 cut(s) 684, 984
BstDEI CTNAG 3 cut(s) 114, 919, 1223
BstDSI CCRYGG 2 cut(s) 1101, 1356
BstF5I GGATG 8 cut(s) 159, 541, 718, 736, 882, 1064, 1141, 1348
BstH2I RGCGCY 1 cut(s) 405
BstHHI GCGC 1 cut(s) 404
BstKTI GATC 3 cut(s) 27, 186, 918
BstMAI GTCTC 6 cut(s) 124, 413, 686, 1359, 1366, 1408
BstMBI GATC 3 cut(s) 24, 183, 915
BstMWI GCNNNNNNNGC 8 cut(s) 147, 272, 338, 631, 743, 941, 1082, 1301
BstNI CCWGG 1 cut(s) 1180
BstSCI CCNGG 8 cut(s) 192, 193, 332, 357, 1042, 1178, 1258, 1411
BstSFI CTRYAG 2 cut(s) 588, 1017
BstX2I RGATCY 1 cut(s) 183
BstYI RGATCY 1 cut(s) 183
BsuRI GGCC 1 cut(s) 119
BtgI CCRYGG 2 cut(s) 1101, 1356
BtsCI GGATG 8 cut(s) 159, 541, 718, 736, 882, 1064, 1141, 1348
BtsIMutI CAGTG 1 cut(s) 663
Cac8I GCNNGC 2 cut(s) 684, 984
CfoI GCGC 1 cut(s) 404
Cfr13I GGNCC 2 cut(s) 367, 815
Cfr9I CCCGGG 1 cut(s) 193
Csp6I GTAC 4 cut(s) 320, 555, 965, 1291
CviAII CATG 3 cut(s) 1102, 1143, 1357
CviQI GTAC 4 cut(s) 320, 555, 965, 1291
DdeI CTNAG 3 cut(s) 114, 919, 1223
DpnI GATC 3 cut(s) 26, 185, 917
DpnII GATC 3 cut(s) 24, 183, 915
Eam1104I CTCTTC 1 cut(s) 1380
EarI CTCTTC 1 cut(s) 1380
Eco130I CCWWGG 2 cut(s) 1101, 1356
Eco24I GRGCYC 1 cut(s) 201
Eco31I GGTCTC 3 cut(s) 124, 413, 1359
Eco47I GGWCC 2 cut(s) 367, 815
Eco72I CACGTG 1 cut(s) 938
Eco88I CYCGRG 1 cut(s) 193
EcoRI GAATTC 2 cut(s) 644, 898
EcoRII CCWGG 1 cut(s) 1178
EcoT14I CCWWGG 2 cut(s) 1101, 1356
EcoT22I ATGCAT 2 cut(s) 622, 708
EcoT38I GRGCYC 1 cut(s) 201
ErhI CCWWGG 2 cut(s) 1101, 1356
Esp3I CGTCTC 1 cut(s) 1366
FaeI CATG 3 cut(s) 1105, 1146, 1360
FalI AAGNNNNNCTT 2 cut(s) 1211, 1243
FaqI GGGAC 1 cut(s) 1325
FatI CATG 3 cut(s) 1101, 1142, 1356
FauNDI CATATG 1 cut(s) 702
Fnu4HI GCNGC 1 cut(s) 1409
FokI GGATG 8 cut(s) 146, 548, 725, 743, 889, 1051, 1148, 1355
FriOI GRGCYC 1 cut(s) 201
Fsp4HI GCNGC 1 cut(s) 1409
FspBI CTAG 1 cut(s) 267
GlaI GCGC 1 cut(s) 403
GluI GCNGC 1 cut(s) 1409
GsuI CTGGAG 1 cut(s) 435
HaeII RGCGCY 1 cut(s) 405
HaeIII GGCC 1 cut(s) 119
HhaI GCGC 1 cut(s) 404
Hin1II CATG 3 cut(s) 1105, 1146, 1360
Hin6I GCGC 1 cut(s) 402
HinP1I GCGC 1 cut(s) 402
HindIII AAGCTT 1 cut(s) 1220
HphI GGTGA 3 cut(s) 115, 626, 1126
Hpy166II GTNNAC 2 cut(s) 1272, 1291
Hpy188I TCNGA 6 cut(s) 24, 183, 297, 454, 769, 922
Hpy188III TCNNGA 2 cut(s) 16, 1319
Hpy8I GTNNAC 2 cut(s) 1272, 1291
HpyAV CCTTC 5 cut(s) 153, 421, 483, 785, 1316
HpyCH4III ACNGT 3 cut(s) 310, 614, 1316
HpyCH4IV ACGT 1 cut(s) 937
HpyCH4V TGCA 7 cut(s) 177, 389, 590, 620, 706, 976, 1085
HpyF10VI GCNNNNNNNGC 8 cut(s) 147, 272, 338, 631, 743, 941, 1082, 1301
HpyF3I CTNAG 3 cut(s) 114, 919, 1223
HpySE526I ACGT 1 cut(s) 937
Hsp92II CATG 3 cut(s) 1105, 1146, 1360
HspAI GCGC 1 cut(s) 402
Kpn2I TCCGGA 1 cut(s) 1318
Kzo9I GATC 3 cut(s) 24, 183, 915
LmnI GCTCC 2 cut(s) 155, 204
LweI GCATC 4 cut(s) 319, 398, 607, 867
MaeI CTAG 1 cut(s) 267
MaeII ACGT 1 cut(s) 937
MaeIII GTNAC 3 cut(s) 217, 304, 1088
MalI GATC 3 cut(s) 26, 185, 917
MboI GATC 3 cut(s) 24, 183, 915
MboII GAAGA 5 cut(s) 74, 256, 650, 1129, 1397
MfeI CAATTG 1 cut(s) 834
MflI RGATCY 1 cut(s) 183
MhlI GDGCHC 1 cut(s) 201
MlyI GAGTC 4 cut(s) 28, 458, 1254, 1361
MmeI TCCRAC 4 cut(s) 253, 432, 1188, 1342
MnlI CCTC 6 cut(s) 182, 264, 727, 934, 1050, 1197
Mph1103I ATGCAT 2 cut(s) 622, 708
MroI TCCGGA 1 cut(s) 1318
MseI TTAA 5 cut(s) 258, 288, 479, 780, 1110
MslI CAYNNNNRTG 2 cut(s) 327, 711
MspCI CTTAAG 1 cut(s) 287
MspR9I CCNGG 8 cut(s) 194, 195, 334, 359, 1044, 1180, 1260, 1413
MunI CAATTG 1 cut(s) 834
Mva1269I GAATGC 1 cut(s) 659
MvaI CCWGG 1 cut(s) 1180
MwoI GCNNNNNNNGC 8 cut(s) 147, 272, 338, 631, 743, 941, 1082, 1301
NciI CCSGG 7 cut(s) 194, 195, 334, 359, 1044, 1260, 1413
NcoI CCATGG 2 cut(s) 1101, 1356
NdeI CATATG 1 cut(s) 702
NdeII GATC 3 cut(s) 24, 183, 915
NlaIII CATG 3 cut(s) 1105, 1146, 1360
NlaIV GGNNCC 3 cut(s) 185, 200, 987
NmuCI GTSAC 2 cut(s) 217, 1088
NsiI ATGCAT 2 cut(s) 622, 708
PctI GAATGC 1 cut(s) 659
PfeI GAWTC 7 cut(s) 106, 292, 434, 584, 758, 957, 1263
PflMI CCANNNNNTGG 1 cut(s) 430
PkrI GCNGC 1 cut(s) 1410
PleI GAGTC 4 cut(s) 27, 457, 1253, 1360
PmaCI CACGTG 1 cut(s) 938
PmlI CACGTG 1 cut(s) 938
PpsI GAGTC 4 cut(s) 27, 457, 1253, 1360
Ppu21I YACGTR 1 cut(s) 938
PsiI TTATAA 1 cut(s) 824
Psp6I CCWGG 1 cut(s) 1178
PspCI CACGTG 1 cut(s) 938
PspGI CCWGG 1 cut(s) 1178
PspN4I GGNNCC 3 cut(s) 185, 200, 987
PspPI GGNCC 2 cut(s) 367, 815
PstI CTGCAG 1 cut(s) 592
PsuI RGATCY 1 cut(s) 183
RsaI GTAC 4 cut(s) 321, 556, 966, 1292
RsaNI GTAC 4 cut(s) 320, 555, 965, 1291
RseI CAYNNNNRTG 2 cut(s) 327, 711
SaqAI TTAA 5 cut(s) 258, 288, 479, 780, 1110
SatI GCNGC 1 cut(s) 1409
Sau3AI GATC 3 cut(s) 24, 183, 915
Sau96I GGNCC 2 cut(s) 367, 815
ScaI AGTACT 1 cut(s) 556
SchI GAGTC 4 cut(s) 28, 458, 1254, 1361
ScrFI CCNGG 8 cut(s) 194, 195, 334, 359, 1044, 1180, 1260, 1413
SduI GDGCHC 1 cut(s) 201
SfaNI GCATC 4 cut(s) 319, 398, 607, 867
SfcI CTRYAG 2 cut(s) 588, 1017
SinI GGWCC 2 cut(s) 367, 815
SmaI CCCGGG 1 cut(s) 195
SmiMI CAYNNNNRTG 2 cut(s) 327, 711
SmlI CTYRAG 2 cut(s) 287, 1201
SmoI CTYRAG 2 cut(s) 287, 1201
SsiI CCGC 2 cut(s) 1304, 1409
SspMI CTAG 1 cut(s) 267
StyD4I CCNGG 8 cut(s) 192, 193, 332, 357, 1042, 1178, 1258, 1411
StyI CCWWGG 2 cut(s) 1101, 1356
TaaI ACNGT 3 cut(s) 310, 614, 1316
TaiI ACGT 1 cut(s) 940
TaqI TCGA 3 cut(s) 354, 608, 1350
TatI WGTACW 3 cut(s) 319, 554, 964
TauI GCSGC 1 cut(s) 1411
TfiI GAWTC 7 cut(s) 106, 292, 434, 584, 758, 957, 1263
Tru1I TTAA 5 cut(s) 258, 288, 479, 780, 1110
Tru9I TTAA 5 cut(s) 258, 288, 479, 780, 1110
TscAI CASTG 1 cut(s) 670
TseFI GTSAC 2 cut(s) 217, 1088
Tsp45I GTSAC 2 cut(s) 217, 1088
TspDTI ATGAA 6 cut(s) 465, 637, 657, 750, 1026, 1251
TspMI CCCGGG 1 cut(s) 193
TspRI CASTG 1 cut(s) 670
Van91I CCANNNNNTGG 1 cut(s) 430
Vha464I CTTAAG 1 cut(s) 287
VpaK11BI GGWCC 2 cut(s) 367, 815
XapI RAATTY 4 cut(s) 472, 644, 898, 1120
XmaI CCCGGG 1 cut(s) 193
XspI CTAG 1 cut(s) 267
ZrmI AGTACT 1 cut(s) 556
Zsp2I ATGCAT 2 cut(s) 622, 708
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.