Rw0G001970

DTW

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00062
OG0012344
Physical Location & Seq
Reverse (-)
82 .. 1378
1297 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G001970.1

Sequence Viewer

Length: 1260 bp
ATGGTGATTCCTACTCAGGCCATTTCCAAGAGACCCATTTGCCCTTCCTGCTCCAAACCCATCCGGCTCTGCCTCTGCAATCGGATCCACAACCCGGGGCTCCACAACTCTGTAAGTGTGACCATTCTCCAACACAGCTTTGAGAAGATCCACCCTCTTAATTCAGCTAGGATTGCCAGATTAGGGCTTAAGAATCTGAAAGTGGTTACTGTTTTTGATGTACATTTTGATGCCCGGTTCGCTATCCGGTTGCTCGACCCGGATTTGGAAACGGGTTTGGACCGGAATGGTTCAAAGGGTGCATCTTTTGATGGAGCTGTGGCAACTGGAGAGACCCAGAAGGTGGATTCTGGGTTTTGTGAGTCGGAAATTGGGGGTTTTGAAAATTCATCAAAACAGAGAAGGTGTAGTAGTGTGGTTGATTGTAATGAGCAATGTAGTTTTGGGGATGATATAGGGGATATGAGTAGTGCTCAAAATCAATTAGGGGGTGTAGATTCTGCAGAAAATTGTAGTGTATCGAACGGTGATGCATTGCTTGTAGAGCAAGAAGATGAATTCATTAGTGAAGCATTCACTGATGGAAAGAGTGTGCTTGCTTTGGAGACTTGCCCTATGCATTTGGATGGACAATCCAATGTGGATGAGGCTTCAACTGCTACGACTTTGGATTCATTATCTGATAGAGATTTTATTCTTAATGTCTTGAAGGTGTCAAATAATGCTGGACCTGTTATAACTGCTACAATTGGCAAGAATGGTGTCATTAGCTCTCTTACTCATACTTGGATGCCACAAACCCATCACAAGAATTCACAATTTGATACGATCTCAGACATTAAAGAAGCGCGTGAGGCTCTGGCAAAGGGATTCGTTGTACAGAAGTTGCAAAAGCAGGCACCTAAAGGAAATGTTGAGTTAGATGAAGCTGTAGAGTTTGAAGTAGAAGTTCCTCCGGGAGTTAGTGCAAGTGACCTTGAAGCCATGGATATTAAGGTGAAGAATTTGATTGTCTTGGATGGAACATGGTCAAAGGCAAAGAGAATGTATGTGGAAAACCCCTGGTTGAAGCTATTGCCACACTTGAGGTTGAATTTGGACAAGCTGAGCTTGTACAATGAAGTGAGAGTCCAGCCCAAGCCGGGATTTCTGTCCACCATTGAGAGCATTGTATACGCACTAAAAGCGGTAGGGGACAGTCCCGAAGGGCTGGACAATCTCTTGGATGTTTTCGAGTCCATGGTTGGCGACCAGAGCGAT
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DTW

Protein Analysis

420

Amino Acids

45.7

Weight (kDa)

4.97

Isoelectric Point (pI)

32.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DTW PF03942 11 - 74 2.4e-08 DTW domain
DTW PF03942 325 - 418 1.1e-23 DTW domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012344)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G54880
fragaria_vesca FvH4_3g22471
malus_domestica MD03G1204100.v1.1 MD11G1220900.v1.1
prunus_persica Prupe.4G211300_v2.0.a1
pyrus_communis pycom03g15470 pycom11g19490
rosa_chinensis RchiOBHm_Chr5g0039141
rosa_laevigata RLG00000033897
rosa_multiflora Rmu_sc0000533.1_g000008 Rmu_ssc0000410.1_g000018 Rmu_ssc0000410.1_g000044
rosa_roxburghii Rroxscaffold_1G00040360
rosa_rugosa Rorug05G0177000
rosa_samantha Rh5BG268300 Rh5CG301100 Rh5DG276100
rosa_wichuraiana Rw0G001970 Rw5G024630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 737
AccB1I GGYRCC 1 cut(s) 898
AccB7I CCANNNNNTGG 1 cut(s) 343
AccI GTMKAC 1 cut(s) 1173
AccII CGCG 1 cut(s) 850
AciI CCGC 1 cut(s) 1187
AclWI GGATC 3 cut(s) 79, 92, 142
AcsI RAATTY 5 cut(s) 385, 557, 811, 1003, 1093
AfaI GTAC 3 cut(s) 222, 879, 1115
AfiI CCNNNNNNNGG 5 cut(s) 94, 183, 265, 343, 1142
AflII CTTAAG 1 cut(s) 188
AgsI TTSAA 8 cut(s) 294, 383, 654, 709, 941, 980, 1069, 1093
AjnI CCWGG 1 cut(s) 1061
AluBI AGCT 8 cut(s) 138, 167, 317, 771, 929, 1072, 1105, 1110
AluI AGCT 8 cut(s) 138, 167, 317, 771, 929, 1072, 1105, 1110
Alw21I GWGCWC 1 cut(s) 475
Alw26I GTCTC 3 cut(s) 25, 326, 599
AlwI GGATC 3 cut(s) 79, 92, 142
Ama87I CYCGRG 1 cut(s) 94
AoxI GGCC 1 cut(s) 18
ApoI RAATTY 5 cut(s) 385, 557, 811, 1003, 1093
AspLEI GCGC 1 cut(s) 850
AspS9I GGNCC 2 cut(s) 280, 728
AsuC2I CCSGG 6 cut(s) 95, 96, 235, 260, 957, 1143
AsuHPI GGTGA 3 cut(s) 16, 539, 1009
AvaI CYCGRG 1 cut(s) 94
AvaII GGWCC 2 cut(s) 280, 728
BamHI GGATCC 1 cut(s) 84
BanI GGYRCC 1 cut(s) 898
BanII GRGCYC 1 cut(s) 102
Bbv12I GWGCWC 1 cut(s) 475
BccI CCATC 6 cut(s) 68, 305, 575, 620, 810, 1013
BciT130I CCWGG 1 cut(s) 1063
BcnI CCSGG 6 cut(s) 95, 96, 235, 260, 957, 1143
BcoDI GTCTC 3 cut(s) 25, 326, 599
BfaI CTAG 1 cut(s) 168
BfmI CTRYAG 2 cut(s) 501, 930
BfrI CTTAAG 1 cut(s) 188
BlpI GCTNAGC 1 cut(s) 1106
Bme1390I CCNGG 7 cut(s) 95, 96, 235, 260, 957, 1063, 1143
Bme18I GGWCC 2 cut(s) 280, 728
BmeT110I CYCGRG 1 cut(s) 94
BmgT120I GGNCC 2 cut(s) 280, 728
BmiI GGNNCC 3 cut(s) 86, 101, 900
BmrFI CCNGG 7 cut(s) 95, 96, 235, 260, 957, 1063, 1143
BmsI GCATC 4 cut(s) 220, 311, 520, 780
BplI GAGNNNNNCTC 2 cut(s) 457, 489
BpmI CTGGAG 1 cut(s) 348
Bpu1102I GCTNAGC 1 cut(s) 1106
BpuEI CTTGAG 1 cut(s) 1105
BpuMI CCSGG 6 cut(s) 95, 96, 235, 260, 957, 1143
BsaI GGTCTC 2 cut(s) 25, 326
BsaJI CCNNGG 5 cut(s) 94, 95, 984, 1061, 1239
BsaWI WCCGGW 2 cut(s) 246, 282
Bsc4I CCNNNNNNNGG 5 cut(s) 94, 183, 265, 343, 1142
Bse1I ACTGG 1 cut(s) 331
Bse3DI GCAATG 2 cut(s) 440, 533
BseBI CCWGG 1 cut(s) 1063
BseDI CCNNGG 5 cut(s) 94, 95, 984, 1061, 1239
BseGI GGATG 7 cut(s) 60, 454, 631, 649, 795, 1024, 1231
BseLI CCNNNNNNNGG 5 cut(s) 94, 183, 265, 343, 1142
BseMI GCAATG 2 cut(s) 440, 533
BseMII CTCAG 3 cut(s) 29, 846, 1097
BseNI ACTGG 1 cut(s) 331
Bsh1236I CGCG 1 cut(s) 850
BshFI GGCC 1 cut(s) 20
BshNI GGYRCC 1 cut(s) 898
BsiHKAI GWGCWC 1 cut(s) 475
BsiHKCI CYCGRG 1 cut(s) 94
BsiSI CCGG 8 cut(s) 64, 95, 235, 247, 260, 283, 956, 1142
BslFI GGGAC 2 cut(s) 1185, 1208
BslI CCNNNNNNNGG 5 cut(s) 94, 183, 265, 343, 1142
BsmAI GTCTC 3 cut(s) 25, 326, 599
BsmFI GGGAC 2 cut(s) 1185, 1208
BsmI GAATGC 1 cut(s) 572
BsnI GGCC 1 cut(s) 20
Bso31I GGTCTC 2 cut(s) 25, 326
BsoBI CYCGRG 1 cut(s) 94
Bsp1286I GDGCHC 2 cut(s) 102, 475
Bsp1407I TGTACA 3 cut(s) 220, 877, 1113
Bsp143I GATC 3 cut(s) 84, 147, 828
Bsp1720I GCTNAGC 1 cut(s) 1106
Bsp19I CCATGG 2 cut(s) 984, 1239
BspACI CCGC 1 cut(s) 1187
BspANI GGCC 1 cut(s) 20
BspCNI CTCAG 3 cut(s) 28, 845, 1098
BspFNI CGCG 1 cut(s) 850
BspLI GGNNCC 3 cut(s) 86, 101, 900
BspMAI CTGCAG 1 cut(s) 505
BspPI GGATC 3 cut(s) 79, 92, 142
BspT107I GGYRCC 1 cut(s) 898
BspTI CTTAAG 1 cut(s) 188
BspTNI GGTCTC 2 cut(s) 25, 326
BsrDI GCAATG 2 cut(s) 440, 533
BsrGI TGTACA 3 cut(s) 220, 877, 1113
BsrI ACTGG 1 cut(s) 331
BssECI CCNNGG 5 cut(s) 94, 95, 984, 1061, 1239
BssMI GATC 3 cut(s) 84, 147, 828
BssNAI GTATAC 1 cut(s) 1174
BssT1I CCWWGG 2 cut(s) 984, 1239
Bst1107I GTATAC 1 cut(s) 1174
Bst2UI CCWGG 1 cut(s) 1063
Bst4CI ACNGT 3 cut(s) 211, 527, 1199
BstAFI CTTAAG 1 cut(s) 188
BstAUI TGTACA 3 cut(s) 220, 877, 1113
BstC8I GCNNGC 2 cut(s) 597, 897
BstDEI CTNAG 3 cut(s) 15, 832, 1106
BstDSI CCRYGG 2 cut(s) 984, 1239
BstF5I GGATG 7 cut(s) 60, 454, 631, 649, 795, 1024, 1231
BstFNI CGCG 1 cut(s) 850
BstHHI GCGC 1 cut(s) 850
BstKTI GATC 3 cut(s) 87, 150, 831
BstMAI GTCTC 3 cut(s) 25, 326, 599
BstMBI GATC 3 cut(s) 84, 147, 828
BstMWI GCNNNNNNNGC 8 cut(s) 48, 173, 239, 544, 656, 854, 1184, 1254
BstNI CCWGG 1 cut(s) 1063
BstSCI CCNGG 7 cut(s) 93, 94, 233, 258, 955, 1061, 1141
BstSFI CTRYAG 2 cut(s) 501, 930
BstUI CGCG 1 cut(s) 850
BstX2I RGATCY 2 cut(s) 84, 147
BstYI RGATCY 2 cut(s) 84, 147
BstZ17I GTATAC 1 cut(s) 1174
BsuRI GGCC 1 cut(s) 20
BtgI CCRYGG 2 cut(s) 984, 1239
BtsCI GGATG 7 cut(s) 60, 454, 631, 649, 795, 1024, 1231
BtsIMutI CAGTG 1 cut(s) 576
Cac8I GCNNGC 2 cut(s) 597, 897
CfoI GCGC 1 cut(s) 850
Cfr13I GGNCC 2 cut(s) 280, 728
Cfr9I CCCGGG 1 cut(s) 94
Csp6I GTAC 3 cut(s) 221, 878, 1114
CviAII CATG 3 cut(s) 985, 1026, 1240
CviQI GTAC 3 cut(s) 221, 878, 1114
DdeI CTNAG 3 cut(s) 15, 832, 1106
DpnI GATC 3 cut(s) 86, 149, 830
DpnII GATC 3 cut(s) 84, 147, 828
Eco130I CCWWGG 2 cut(s) 984, 1239
Eco24I GRGCYC 1 cut(s) 102
Eco31I GGTCTC 2 cut(s) 25, 326
Eco47I GGWCC 2 cut(s) 280, 728
Eco88I CYCGRG 1 cut(s) 94
EcoRI GAATTC 2 cut(s) 557, 811
EcoRII CCWGG 1 cut(s) 1061
EcoT14I CCWWGG 2 cut(s) 984, 1239
EcoT22I ATGCAT 2 cut(s) 535, 621
EcoT38I GRGCYC 1 cut(s) 102
ErhI CCWWGG 2 cut(s) 984, 1239
FaeI CATG 3 cut(s) 988, 1029, 1243
FalI AAGNNNNNCTT 2 cut(s) 1094, 1126
FaqI GGGAC 2 cut(s) 1185, 1208
FatI CATG 3 cut(s) 984, 1025, 1239
FblI GTMKAC 1 cut(s) 1173
FokI GGATG 7 cut(s) 47, 461, 638, 656, 802, 1031, 1238
FriOI GRGCYC 1 cut(s) 102
FspBI CTAG 1 cut(s) 168
GlaI GCGC 1 cut(s) 849
GsuI CTGGAG 1 cut(s) 348
HaeIII GGCC 1 cut(s) 20
HapII CCGG 8 cut(s) 64, 95, 235, 247, 260, 283, 956, 1142
HhaI GCGC 1 cut(s) 850
Hin1II CATG 3 cut(s) 988, 1029, 1243
Hin6I GCGC 1 cut(s) 848
HinP1I GCGC 1 cut(s) 848
HinfI GANTC 9 cut(s) 7, 193, 347, 362, 497, 671, 870, 1128, 1235
HpaII CCGG 8 cut(s) 64, 95, 235, 247, 260, 283, 956, 1142
HphI GGTGA 3 cut(s) 16, 539, 1009
Hpy166II GTNNAC 2 cut(s) 1155, 1174
Hpy188I TCNGA 5 cut(s) 84, 198, 367, 682, 835
Hpy188III TCNNGA 2 cut(s) 706, 1202
Hpy8I GTNNAC 2 cut(s) 1155, 1174
HpyAV CCTTC 5 cut(s) 54, 334, 396, 703, 1199
HpyCH4III ACNGT 3 cut(s) 211, 527, 1199
HpyCH4V TGCA 7 cut(s) 78, 302, 503, 533, 619, 889, 968
HpyF10VI GCNNNNNNNGC 8 cut(s) 48, 173, 239, 544, 656, 854, 1184, 1254
HpyF3I CTNAG 3 cut(s) 15, 832, 1106
Hsp92II CATG 3 cut(s) 988, 1029, 1243
HspAI GCGC 1 cut(s) 848
Kzo9I GATC 3 cut(s) 84, 147, 828
LmnI GCTCC 3 cut(s) 56, 105, 314
LweI GCATC 4 cut(s) 220, 311, 520, 780
MaeI CTAG 1 cut(s) 168
MaeIII GTNAC 3 cut(s) 118, 205, 971
MalI GATC 3 cut(s) 86, 149, 830
MboI GATC 3 cut(s) 84, 147, 828
MboII GAAGA 3 cut(s) 157, 563, 1012
MfeI CAATTG 1 cut(s) 747
MflI RGATCY 2 cut(s) 84, 147
MhlI GDGCHC 2 cut(s) 102, 475
MlyI GAGTC 3 cut(s) 371, 1137, 1244
MmeI TCCRAC 2 cut(s) 154, 345
MnlI CCTC 6 cut(s) 83, 165, 640, 847, 963, 1080
Mph1103I ATGCAT 2 cut(s) 535, 621
MseI TTAA 5 cut(s) 159, 189, 699, 840, 993
MslI CAYNNNNRTG 2 cut(s) 228, 624
MspCI CTTAAG 1 cut(s) 188
MspI CCGG 8 cut(s) 64, 95, 235, 247, 260, 283, 956, 1142
MspR9I CCNGG 7 cut(s) 95, 96, 235, 260, 957, 1063, 1143
MunI CAATTG 1 cut(s) 747
Mva1269I GAATGC 1 cut(s) 572
MvaI CCWGG 1 cut(s) 1063
MvnI CGCG 1 cut(s) 850
MwoI GCNNNNNNNGC 8 cut(s) 48, 173, 239, 544, 656, 854, 1184, 1254
NciI CCSGG 6 cut(s) 95, 96, 235, 260, 957, 1143
NcoI CCATGG 2 cut(s) 984, 1239
NdeII GATC 3 cut(s) 84, 147, 828
NlaIII CATG 3 cut(s) 988, 1029, 1243
NlaIV GGNNCC 3 cut(s) 86, 101, 900
NmuCI GTSAC 2 cut(s) 118, 971
NsiI ATGCAT 2 cut(s) 535, 621
PctI GAATGC 1 cut(s) 572
PfeI GAWTC 6 cut(s) 7, 193, 347, 497, 671, 870
PflMI CCANNNNNTGG 1 cut(s) 343
PfoI TCCNGGA 1 cut(s) 955
PleI GAGTC 3 cut(s) 370, 1136, 1243
PpsI GAGTC 3 cut(s) 370, 1136, 1243
PsiI TTATAA 1 cut(s) 737
Psp6I CCWGG 1 cut(s) 1061
PspGI CCWGG 1 cut(s) 1061
PspN4I GGNNCC 3 cut(s) 86, 101, 900
PspPI GGNCC 2 cut(s) 280, 728
PstI CTGCAG 1 cut(s) 505
PsuI RGATCY 2 cut(s) 84, 147
RsaI GTAC 3 cut(s) 222, 879, 1115
RsaNI GTAC 3 cut(s) 221, 878, 1114
RseI CAYNNNNRTG 2 cut(s) 228, 624
SaqAI TTAA 5 cut(s) 159, 189, 699, 840, 993
Sau3AI GATC 3 cut(s) 84, 147, 828
Sau96I GGNCC 2 cut(s) 280, 728
SchI GAGTC 3 cut(s) 371, 1137, 1244
ScrFI CCNGG 7 cut(s) 95, 96, 235, 260, 957, 1063, 1143
SduI GDGCHC 2 cut(s) 102, 475
SfaNI GCATC 4 cut(s) 220, 311, 520, 780
SfcI CTRYAG 2 cut(s) 501, 930
SinI GGWCC 2 cut(s) 280, 728
SmaI CCCGGG 1 cut(s) 96
SmiMI CAYNNNNRTG 2 cut(s) 228, 624
SmlI CTYRAG 2 cut(s) 188, 1084
SmoI CTYRAG 2 cut(s) 188, 1084
SsiI CCGC 1 cut(s) 1187
SspMI CTAG 1 cut(s) 168
StyD4I CCNGG 7 cut(s) 93, 94, 233, 258, 955, 1061, 1141
StyI CCWWGG 2 cut(s) 984, 1239
TaaI ACNGT 3 cut(s) 211, 527, 1199
TaqI TCGA 3 cut(s) 255, 521, 1233
TatI WGTACW 3 cut(s) 220, 877, 1113
TfiI GAWTC 6 cut(s) 7, 193, 347, 497, 671, 870
Tru1I TTAA 5 cut(s) 159, 189, 699, 840, 993
Tru9I TTAA 5 cut(s) 159, 189, 699, 840, 993
TscAI CASTG 1 cut(s) 583
TseFI GTSAC 2 cut(s) 118, 971
Tsp45I GTSAC 2 cut(s) 118, 971
TspDTI ATGAA 6 cut(s) 378, 550, 570, 663, 939, 1134
TspMI CCCGGG 1 cut(s) 94
TspRI CASTG 1 cut(s) 583
Van91I CCANNNNNTGG 1 cut(s) 343
Vha464I CTTAAG 1 cut(s) 188
VpaK11BI GGWCC 2 cut(s) 280, 728
XapI RAATTY 5 cut(s) 385, 557, 811, 1003, 1093
XmaI CCCGGG 1 cut(s) 94
XmiI GTMKAC 1 cut(s) 1173
XspI CTAG 1 cut(s) 168
Zsp2I ATGCAT 2 cut(s) 535, 621
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.