RLG00000033897

DTW

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0012344
Physical Location & Seq
Forward (+)
33858246 .. 33859565
1320 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033897

Sequence Viewer

Length: 1320 bp
ATGGTGACTCCTTCTCAGGCCATTTCCAAGAGACCGACTTGCCCTTCCTGCTCCAAACCCGTCCGGCTCTGCCTCTGCAATCGGATCCACAACCCGGGGCTCCACAACTCTGTAAGTGTGACCATTCTTCAACACAGCTATGAGAAGAACCACCCACTTAATTCAGCTAGGATTGCCAGATTAGGGCTTAAGAATCTGAAAGTGGTTACTGTTTTTGATGTACATTTTGATGCCCGGTTCGTTTTCCGGTTGCTTGACCCGGATTCGAACCGGAATGGTTCAAAGGGTGCATCTTTTGATGGAGCTGTGGAAACTGGAGAGACCCAGAAGGTGGATTCTGGGTTTTGTGAGTCGGAAATTGGGGGTTTTGAAAATTCATCAAAACAGAGAAGGTGTAGTAGTGTGGTTGATTGTAATGAGCAATGTAGTTTTGGGAATGATGTAGGTGATATGAGTATTGCTCAAAATCAATTAGGAAGTGTAGATTCTGCAGAAAATTGTAGTGTATCGAACTGTGATGCATTGCTTGTAGAGCAAGAAGCTGAATTCATTAGTGAAGAATTCATCGATGGAAAGAGTGTGCTTGCTTTGGAGACTTGTCATATGCATTTGGATGGACAATCCAATGTGGATGAGGCTTCAACTGCTACAACTTTGGATTCATTATCTGATAGAGATTTTAATTTGGTTGAAGGTGTCAAAGTTAGTAATCCTGGACCTGTTATAACGGCTACAATTGGCAAGAATGGTGTCATTAGCTCTCTTACTCATAATTGGATGCCACAAACCCATCACAAGAATTCACAATTTGATACGATCTCAGACATTACAGAAGCGCGTGAGGCTCTGGCAAAGGGATTCGTTGTACAAAAGTTGCAAAAGCAGCCACCTAAAGGAAATGTAGAGTTAGATGAAGCTGTAGAGTTTGAAGTAGAAGTTCCTCCAGGGTCTGTTCTCTTGTTTCCATCCAAAGAAGCAGTTAGTGCTAGTGACCTTGAAGCCATGGATATTAAGGTGAAGAATTTGATTGTCTTGGATGGAACATGGTCAAAGGCAAAGAGAATGTATGTGGAAAATCCCTGGTTGAAGCTGTTGCCACACTTGAGGTTGGATTTGGACAAGCTGAGCTTGTACAATGAAGTGAGAGTCCAGCCCAAGCCTGGATTCTTGTCCACCATTGAGAGCATTGTGTACGCACTAAAGGCAGTAGGGGACAGTCCGGAAGGGCTGGACAATCTCTTGGATGTTTTCGAGTCCATGGTTGGAGACCAGAGACGATGTAAAGAAGAGAGATTGAGCAATGTCTCGCCGCTCGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DTW

Protein Analysis

440

Amino Acids

48.09

Weight (kDa)

5.15

Isoelectric Point (pI)

38.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DTW PF03942 11 - 72 1e-09 DTW domain
DTW PF03942 258 - 424 9.3e-28 DTW domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012344)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G54880
fragaria_vesca FvH4_3g22471
malus_domestica MD03G1204100.v1.1 MD11G1220900.v1.1
prunus_persica Prupe.4G211300_v2.0.a1
pyrus_communis pycom03g15470 pycom11g19490
rosa_chinensis RchiOBHm_Chr5g0039141
rosa_laevigata RLG00000033897
rosa_multiflora Rmu_sc0000533.1_g000008 Rmu_ssc0000410.1_g000018 Rmu_ssc0000410.1_g000044
rosa_roxburghii Rroxscaffold_1G00040360
rosa_rugosa Rorug05G0177000
rosa_samantha Rh5BG268300 Rh5CG301100 Rh5DG276100
rosa_wichuraiana Rw0G001970 Rw5G024630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 725
AccB7I CCANNNNNTGG 1 cut(s) 331
AccBSI CCGCTC 1 cut(s) 1312
AccII CGCG 1 cut(s) 838
AccIII TCCGGA 1 cut(s) 1219
AciI CCGC 1 cut(s) 1310
AclWI GGATC 2 cut(s) 79, 92
AcsI RAATTY 5 cut(s) 373, 545, 560, 799, 1021
AfaI GTAC 4 cut(s) 222, 867, 1133, 1193
AfiI CCNNNNNNNGG 5 cut(s) 94, 183, 331, 893, 1160
AflII CTTAAG 1 cut(s) 188
AgsI TTSAA 8 cut(s) 131, 282, 371, 642, 692, 929, 998, 1087
AjnI CCWGG 4 cut(s) 712, 943, 1079, 1159
AjuI GAANNNNNNNTTGG 2 cut(s) 1148, 1180
AluBI AGCT 9 cut(s) 138, 167, 305, 542, 759, 917, 1090, 1123, 1128
AluI AGCT 9 cut(s) 138, 167, 305, 542, 759, 917, 1090, 1123, 1128
Alw26I GTCTC 6 cut(s) 25, 314, 587, 1260, 1267, 1309
AlwI GGATC 2 cut(s) 79, 92
AlwNI CAGNNNCTG 1 cut(s) 950
Ama87I CYCGRG 1 cut(s) 94
Aor13HI TCCGGA 1 cut(s) 1219
AoxI GGCC 1 cut(s) 18
ApeKI GCWGC 1 cut(s) 883
ApoI RAATTY 5 cut(s) 373, 545, 560, 799, 1021
AspLEI GCGC 1 cut(s) 838
AspS9I GGNCC 1 cut(s) 716
AsuC2I CCSGG 4 cut(s) 95, 96, 235, 260
AsuHPI GGTGA 3 cut(s) 16, 458, 1027
AsuII TTCGAA 1 cut(s) 266
AvaI CYCGRG 1 cut(s) 94
AvaII GGWCC 1 cut(s) 716
BamHI GGATCC 1 cut(s) 84
BanII GRGCYC 1 cut(s) 102
BbvI GCAGC 1 cut(s) 895
BccI CCATC 6 cut(s) 293, 563, 608, 798, 973, 1031
BceAI ACGGC 1 cut(s) 744
BciT130I CCWGG 4 cut(s) 714, 945, 1081, 1161
BcnI CCSGG 4 cut(s) 95, 96, 235, 260
BcoDI GTCTC 6 cut(s) 25, 314, 587, 1260, 1267, 1309
BfaI CTAG 2 cut(s) 168, 987
BfmI CTRYAG 2 cut(s) 489, 918
BfrI CTTAAG 1 cut(s) 188
BisI GCNGC 2 cut(s) 884, 1310
BlpI GCTNAGC 1 cut(s) 1124
BlsI GCNGC 2 cut(s) 885, 1311
Bme1390I CCNGG 8 cut(s) 95, 96, 235, 260, 714, 945, 1081, 1161
Bme18I GGWCC 1 cut(s) 716
BmeT110I CYCGRG 1 cut(s) 94
BmgT120I GGNCC 1 cut(s) 716
BmiI GGNNCC 2 cut(s) 86, 101
BmrFI CCNGG 8 cut(s) 95, 96, 235, 260, 714, 945, 1081, 1161
BmsI GCATC 4 cut(s) 220, 299, 508, 768
BplI GAGNNNNNCTC 2 cut(s) 445, 477
BpmI CTGGAG 2 cut(s) 336, 927
Bpu1102I GCTNAGC 1 cut(s) 1124
Bpu14I TTCGAA 1 cut(s) 266
BpuEI CTTGAG 1 cut(s) 1123
BpuMI CCSGG 4 cut(s) 95, 96, 235, 260
Bsa29I ATCGAT 1 cut(s) 567
BsaI GGTCTC 3 cut(s) 25, 314, 1260
BsaJI CCNNGG 6 cut(s) 94, 95, 944, 1002, 1079, 1257
BsaWI WCCGGW 3 cut(s) 246, 270, 1219
Bsc4I CCNNNNNNNGG 5 cut(s) 94, 183, 331, 893, 1160
Bse1I ACTGG 1 cut(s) 319
Bse3DI GCAATG 3 cut(s) 428, 521, 1306
BseAI TCCGGA 1 cut(s) 1219
BseBI CCWGG 4 cut(s) 714, 945, 1081, 1161
BseCI ATCGAT 1 cut(s) 567
BseDI CCNNGG 6 cut(s) 94, 95, 944, 1002, 1079, 1257
BseGI GGATG 6 cut(s) 619, 637, 783, 965, 1042, 1249
BseLI CCNNNNNNNGG 5 cut(s) 94, 183, 331, 893, 1160
BseMI GCAATG 3 cut(s) 428, 521, 1306
BseMII CTCAG 3 cut(s) 29, 834, 1115
BseNI ACTGG 1 cut(s) 319
BseXI GCAGC 1 cut(s) 895
Bsh1236I CGCG 1 cut(s) 838
BshFI GGCC 1 cut(s) 20
BshVI ATCGAT 1 cut(s) 567
BsiHKCI CYCGRG 1 cut(s) 94
BsiSI CCGG 7 cut(s) 64, 95, 235, 247, 260, 271, 1220
BslFI GGGAC 1 cut(s) 1226
BslI CCNNNNNNNGG 5 cut(s) 94, 183, 331, 893, 1160
BsmAI GTCTC 6 cut(s) 25, 314, 587, 1260, 1267, 1309
BsmBI CGTCTC 1 cut(s) 1267
BsmFI GGGAC 1 cut(s) 1226
BsnI GGCC 1 cut(s) 20
Bso31I GGTCTC 3 cut(s) 25, 314, 1260
BsoBI CYCGRG 1 cut(s) 94
Bsp119I TTCGAA 1 cut(s) 266
Bsp1286I GDGCHC 1 cut(s) 102
Bsp13I TCCGGA 1 cut(s) 1219
Bsp1407I TGTACA 3 cut(s) 220, 865, 1131
Bsp143I GATC 2 cut(s) 84, 816
Bsp1720I GCTNAGC 1 cut(s) 1124
Bsp19I CCATGG 2 cut(s) 1002, 1257
BspACI CCGC 1 cut(s) 1310
BspANI GGCC 1 cut(s) 20
BspCNI CTCAG 3 cut(s) 28, 833, 1116
BspDI ATCGAT 1 cut(s) 567
BspEI TCCGGA 1 cut(s) 1219
BspFNI CGCG 1 cut(s) 838
BspLI GGNNCC 2 cut(s) 86, 101
BspMAI CTGCAG 1 cut(s) 493
BspPI GGATC 2 cut(s) 79, 92
BspT104I TTCGAA 1 cut(s) 266
BspTI CTTAAG 1 cut(s) 188
BspTNI GGTCTC 3 cut(s) 25, 314, 1260
BsrBI CCGCTC 1 cut(s) 1312
BsrDI GCAATG 3 cut(s) 428, 521, 1306
BsrGI TGTACA 3 cut(s) 220, 865, 1131
BsrI ACTGG 1 cut(s) 319
BssECI CCNNGG 6 cut(s) 94, 95, 944, 1002, 1079, 1257
BssMI GATC 2 cut(s) 84, 816
BssT1I CCWWGG 2 cut(s) 1002, 1257
Bst2UI CCWGG 4 cut(s) 714, 945, 1081, 1161
Bst4CI ACNGT 3 cut(s) 211, 515, 1217
Bst6I CTCTTC 1 cut(s) 1281
BstAFI CTTAAG 1 cut(s) 188
BstAPI GCANNNNNTGC 1 cut(s) 983
BstAUI TGTACA 3 cut(s) 220, 865, 1131
BstBI TTCGAA 1 cut(s) 266
BstC8I GCNNGC 1 cut(s) 585
BstDEI CTNAG 3 cut(s) 15, 820, 1124
BstDSI CCRYGG 2 cut(s) 1002, 1257
BstF5I GGATG 6 cut(s) 619, 637, 783, 965, 1042, 1249
BstFNI CGCG 1 cut(s) 838
BstHHI GCGC 1 cut(s) 838
BstKTI GATC 2 cut(s) 87, 819
BstMAI GTCTC 6 cut(s) 25, 314, 587, 1260, 1267, 1309
BstMBI GATC 2 cut(s) 84, 816
BstMWI GCNNNNNNNGC 8 cut(s) 48, 173, 532, 644, 842, 883, 983, 1202
BstNI CCWGG 4 cut(s) 714, 945, 1081, 1161
BstSCI CCNGG 8 cut(s) 93, 94, 233, 258, 712, 943, 1079, 1159
BstSFI CTRYAG 2 cut(s) 489, 918
BstUI CGCG 1 cut(s) 838
BstV1I GCAGC 1 cut(s) 895
BstX2I RGATCY 1 cut(s) 84
BstYI RGATCY 1 cut(s) 84
Bsu15I ATCGAT 1 cut(s) 567
BsuRI GGCC 1 cut(s) 20
BsuTUI ATCGAT 1 cut(s) 567
BtgI CCRYGG 2 cut(s) 1002, 1257
BtsCI GGATG 6 cut(s) 619, 637, 783, 965, 1042, 1249
Cac8I GCNNGC 1 cut(s) 585
CaiI CAGNNNCTG 1 cut(s) 950
CfoI GCGC 1 cut(s) 838
Cfr13I GGNCC 1 cut(s) 716
Cfr9I CCCGGG 1 cut(s) 94
ClaI ATCGAT 1 cut(s) 567
Csp6I GTAC 4 cut(s) 221, 866, 1132, 1192
CviAII CATG 3 cut(s) 1003, 1044, 1258
CviQI GTAC 4 cut(s) 221, 866, 1132, 1192
DdeI CTNAG 3 cut(s) 15, 820, 1124
DpnI GATC 2 cut(s) 86, 818
DpnII GATC 2 cut(s) 84, 816
Eam1104I CTCTTC 1 cut(s) 1281
EarI CTCTTC 1 cut(s) 1281
Eco130I CCWWGG 2 cut(s) 1002, 1257
Eco24I GRGCYC 1 cut(s) 102
Eco31I GGTCTC 3 cut(s) 25, 314, 1260
Eco47I GGWCC 1 cut(s) 716
Eco88I CYCGRG 1 cut(s) 94
EcoRI GAATTC 3 cut(s) 545, 560, 799
EcoRII CCWGG 4 cut(s) 712, 943, 1079, 1159
EcoT14I CCWWGG 2 cut(s) 1002, 1257
EcoT22I ATGCAT 2 cut(s) 523, 609
EcoT38I GRGCYC 1 cut(s) 102
ErhI CCWWGG 2 cut(s) 1002, 1257
Esp3I CGTCTC 1 cut(s) 1267
FaeI CATG 3 cut(s) 1006, 1047, 1261
FalI AAGNNNNNCTT 2 cut(s) 1112, 1144
FaqI GGGAC 1 cut(s) 1226
FatI CATG 3 cut(s) 1002, 1043, 1257
FauNDI CATATG 1 cut(s) 603
Fnu4HI GCNGC 2 cut(s) 884, 1310
FokI GGATG 6 cut(s) 626, 644, 790, 952, 1049, 1256
FriOI GRGCYC 1 cut(s) 102
Fsp4HI GCNGC 2 cut(s) 884, 1310
FspBI CTAG 2 cut(s) 168, 987
GlaI GCGC 1 cut(s) 837
GluI GCNGC 2 cut(s) 884, 1310
GsuI CTGGAG 2 cut(s) 336, 927
HaeIII GGCC 1 cut(s) 20
HapII CCGG 7 cut(s) 64, 95, 235, 247, 260, 271, 1220
HhaI GCGC 1 cut(s) 838
Hin1II CATG 3 cut(s) 1006, 1047, 1261
Hin6I GCGC 1 cut(s) 836
HinP1I GCGC 1 cut(s) 836
HpaII CCGG 7 cut(s) 64, 95, 235, 247, 260, 271, 1220
HphI GGTGA 3 cut(s) 16, 458, 1027
Hpy166II GTNNAC 2 cut(s) 1173, 1192
Hpy188I TCNGA 5 cut(s) 84, 198, 355, 670, 823
Hpy188III TCNNGA 1 cut(s) 1220
Hpy8I GTNNAC 2 cut(s) 1173, 1192
HpyAV CCTTC 6 cut(s) 21, 54, 322, 384, 686, 1217
HpyCH4III ACNGT 3 cut(s) 211, 515, 1217
HpyCH4V TGCA 6 cut(s) 78, 290, 491, 521, 607, 877
HpyF10VI GCNNNNNNNGC 8 cut(s) 48, 173, 532, 644, 842, 883, 983, 1202
HpyF3I CTNAG 3 cut(s) 15, 820, 1124
Hsp92II CATG 3 cut(s) 1006, 1047, 1261
HspAI GCGC 1 cut(s) 836
Kpn2I TCCGGA 1 cut(s) 1219
Kzo9I GATC 2 cut(s) 84, 816
LmnI GCTCC 3 cut(s) 56, 105, 302
Lsp1109I GCAGC 1 cut(s) 895
LweI GCATC 4 cut(s) 220, 299, 508, 768
MaeI CTAG 2 cut(s) 168, 987
MaeIII GTNAC 4 cut(s) 4, 118, 205, 989
MalI GATC 2 cut(s) 86, 818
MbiI CCGCTC 1 cut(s) 1312
MboI GATC 2 cut(s) 84, 816
MboII GAAGA 5 cut(s) 119, 157, 569, 1030, 1298
MfeI CAATTG 1 cut(s) 735
MflI RGATCY 1 cut(s) 84
MhlI GDGCHC 1 cut(s) 102
MlyI GAGTC 3 cut(s) 359, 1155, 1262
MmeI TCCRAC 3 cut(s) 333, 1089, 1243
MnlI CCTC 5 cut(s) 83, 628, 835, 951, 1098
Mph1103I ATGCAT 2 cut(s) 523, 609
MroI TCCGGA 1 cut(s) 1219
MseI TTAA 4 cut(s) 159, 189, 681, 1011
MslI CAYNNNNRTG 3 cut(s) 138, 228, 612
MspCI CTTAAG 1 cut(s) 188
MspI CCGG 7 cut(s) 64, 95, 235, 247, 260, 271, 1220
MspR9I CCNGG 8 cut(s) 95, 96, 235, 260, 714, 945, 1081, 1161
MunI CAATTG 1 cut(s) 735
MvaI CCWGG 4 cut(s) 714, 945, 1081, 1161
MvnI CGCG 1 cut(s) 838
MwoI GCNNNNNNNGC 8 cut(s) 48, 173, 532, 644, 842, 883, 983, 1202
NciI CCSGG 4 cut(s) 95, 96, 235, 260
NcoI CCATGG 2 cut(s) 1002, 1257
NdeI CATATG 1 cut(s) 603
NdeII GATC 2 cut(s) 84, 816
NlaIII CATG 3 cut(s) 1006, 1047, 1261
NlaIV GGNNCC 2 cut(s) 86, 101
NmeAIII GCCGAG 1 cut(s) 1293
NmuCI GTSAC 3 cut(s) 4, 118, 989
NsiI ATGCAT 2 cut(s) 523, 609
NspV TTCGAA 1 cut(s) 266
PfeI GAWTC 7 cut(s) 193, 263, 335, 485, 659, 858, 1164
PflMI CCANNNNNTGG 1 cut(s) 331
PfoI TCCNGGA 1 cut(s) 712
PkrI GCNGC 2 cut(s) 885, 1311
PleI GAGTC 3 cut(s) 358, 1154, 1261
PpsI GAGTC 3 cut(s) 358, 1154, 1261
PsiI TTATAA 1 cut(s) 725
Psp6I CCWGG 4 cut(s) 712, 943, 1079, 1159
PspGI CCWGG 4 cut(s) 712, 943, 1079, 1159
PspN4I GGNNCC 2 cut(s) 86, 101
PspPI GGNCC 1 cut(s) 716
PstI CTGCAG 1 cut(s) 493
PstNI CAGNNNCTG 1 cut(s) 950
PsuI RGATCY 1 cut(s) 84
RsaI GTAC 4 cut(s) 222, 867, 1133, 1193
RsaNI GTAC 4 cut(s) 221, 866, 1132, 1192
RseI CAYNNNNRTG 3 cut(s) 138, 228, 612
SaqAI TTAA 4 cut(s) 159, 189, 681, 1011
SatI GCNGC 2 cut(s) 884, 1310
Sau3AI GATC 2 cut(s) 84, 816
Sau96I GGNCC 1 cut(s) 716
SchI GAGTC 3 cut(s) 359, 1155, 1262
ScrFI CCNGG 8 cut(s) 95, 96, 235, 260, 714, 945, 1081, 1161
SduI GDGCHC 1 cut(s) 102
SfaNI GCATC 4 cut(s) 220, 299, 508, 768
SfcI CTRYAG 2 cut(s) 489, 918
SfuI TTCGAA 1 cut(s) 266
SinI GGWCC 1 cut(s) 716
SmaI CCCGGG 1 cut(s) 96
SmiMI CAYNNNNRTG 3 cut(s) 138, 228, 612
SmlI CTYRAG 2 cut(s) 188, 1102
SmoI CTYRAG 2 cut(s) 188, 1102
SsiI CCGC 1 cut(s) 1310
SspMI CTAG 2 cut(s) 168, 987
StyD4I CCNGG 8 cut(s) 93, 94, 233, 258, 712, 943, 1079, 1159
StyI CCWWGG 2 cut(s) 1002, 1257
TaaI ACNGT 3 cut(s) 211, 515, 1217
TaqI TCGA 4 cut(s) 266, 509, 567, 1251
TaqII GACCGA 1 cut(s) 49
TatI WGTACW 3 cut(s) 220, 865, 1131
TauI GCSGC 1 cut(s) 1312
TfiI GAWTC 7 cut(s) 193, 263, 335, 485, 659, 858, 1164
Tru1I TTAA 4 cut(s) 159, 189, 681, 1011
Tru9I TTAA 4 cut(s) 159, 189, 681, 1011
TseFI GTSAC 3 cut(s) 4, 118, 989
TseI GCWGC 1 cut(s) 883
Tsp45I GTSAC 3 cut(s) 4, 118, 989
TspDTI ATGAA 6 cut(s) 366, 538, 553, 651, 927, 1152
TspMI CCCGGG 1 cut(s) 94
Van91I CCANNNNNTGG 1 cut(s) 331
Vha464I CTTAAG 1 cut(s) 188
VpaK11BI GGWCC 1 cut(s) 716
XapI RAATTY 5 cut(s) 373, 545, 560, 799, 1021
XcmI CCANNNNNNNNNTGG 1 cut(s) 1157
XmaI CCCGGG 1 cut(s) 94
XspI CTAG 2 cut(s) 168, 987
Zsp2I ATGCAT 2 cut(s) 523, 609
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.