Rh5CG301100

DTW

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0012344
Physical Location & Seq
Forward (+)
33147364 .. 33148713
1350 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG301100.1

Sequence Viewer

Length: 1350 bp
ATGGTGACTCCTACTCAGGCCATTTCCAAGAGACCCATTTGCCCTTCCTGCTCCAAACCCATCCGGCTCTGCCTCTGCAATCGGATCCACAACCCGGGGCTCCACAACTCTGTAAGTGTGACCATTCTCCAACACAGCTTTGAGAAGAACCACCCTCTTAATTCAGCTAGGATTGCCAGGTTAGGGCTTAAGAATCTGAAAGTGGTTACTGTTTTTGATGTACATTTTGATGCCCGGTTCGTTATCCGGTTGCTCGACCCGGATTTGGAAACGGGTTTGGACCGGAATGGTTCAAAGGGTGCATCTTTTGATGGAGCTGTGGAAACTGGAGAGACCCAGAAGGTGGATTCTGGGTTTTGTGAGTCGGAAATTGAGGGTTTTGAAAATTCATCAAAACAGAGAAGGTGTAGTAGTGTGGTTGATTGTAATAAGCAATGTAGTTTTGGGGATGATATAGGGGATATGAGTAGTGCTCAAAATCAATTAGGAAGTGTAGATTCTGCAGAAAATTGTAGCACATCGACTGGTGATGCATTACTTGTAGAGAAAGAAGATGAATTCACCAGTGAAGAGTTCACTGATGGAAAGAGTGTGCTTGCTTTGGAGACTTGTCATAAGCATTTGGATGGACAATCCAATGTGGATGAGGCTTCAACTGCTACGACATTGGATTCACTATCCGATGAACACAAGCTTTCTGATACGGATTTTGATTCAGTTGACAGTGTCAGAGTTAGTAATGCTGGACCTCTTATAACGGCTACAATTGGCAAGAATGGTGTCATTAGCTCTCTTACTCATACTTGGATGCCACAAACCCATCACAAGAATTCACATTTTGATACGATTTCAGACACTACAGAAGCGCGCGAGGCTCTGGCAAAGGGATTCGTTGTACAAAAGTTGCAAAAGCAGCCACCGGAAGGAAATGTAGACATAGATGAAGCTGTAGAGTTTGAAGTAGAAGTTCCTCCGGGGTCTGTTCTCTTGTTTCCATCCAAAGAAGCAGTTGGTGCTAGTGACCTTGAAGCCATGGATATTAAGGTGAAGAATTTGATTGTCTTGGATGGAACATGGTCAAAGGCAAAAAGAATGTATGTGGAAAACCCCTGGTTGAAGCTATTGCCACACTTGAGGTTGGATTTGGACAAGCTGAGCTTGTACAATGAAGTGAGAGTCCAGCCCAAGCCGGGATTCTTGTCCACCATTGAGAGCATTGTGTACGCACTAAAGGCGGTAGGCGACAGTCCGGAAGGGCTGGACAATCTCTTGGATGTTTTCGAGTCCATGGTTGGAGACCAGAGACGATGTAAAGAAGAGAGATTGAGCAATGTATCACCGCCCGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DTW

Protein Analysis

449

Amino Acids

49.11

Weight (kDa)

4.99

Isoelectric Point (pI)

35.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DTW PF03942 11 - 71 7e-09 DTW domain
DTW PF03942 268 - 434 3.8e-27 DTW domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012344)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G54880
fragaria_vesca FvH4_3g22471
malus_domestica MD03G1204100.v1.1 MD11G1220900.v1.1
prunus_persica Prupe.4G211300_v2.0.a1
pyrus_communis pycom03g15470 pycom11g19490
rosa_chinensis RchiOBHm_Chr5g0039141
rosa_laevigata RLG00000033897
rosa_multiflora Rmu_sc0000533.1_g000008 Rmu_ssc0000410.1_g000018 Rmu_ssc0000410.1_g000044
rosa_roxburghii Rroxscaffold_1G00040360
rosa_rugosa Rorug05G0177000
rosa_samantha Rh5BG268300 Rh5CG301100 Rh5DG276100
rosa_wichuraiana Rw0G001970 Rw5G024630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 755
AccB7I CCANNNNNTGG 1 cut(s) 343
AccI GTMKAC 1 cut(s) 933
AccII CGCG 2 cut(s) 868, 870
AccIII TCCGGA 1 cut(s) 1249
AciI CCGC 2 cut(s) 1235, 1340
AclWI GGATC 2 cut(s) 79, 92
AcsI RAATTY 4 cut(s) 385, 557, 829, 1051
AfaI GTAC 4 cut(s) 222, 897, 1163, 1223
AfiI CCNNNNNNNGG 6 cut(s) 94, 183, 265, 343, 923, 1190
AflII CTTAAG 1 cut(s) 188
AgsI TTSAA 6 cut(s) 294, 383, 654, 959, 1028, 1117
AjnI CCWGG 2 cut(s) 176, 1109
AjuI GAANNNNNNNTTGG 2 cut(s) 1178, 1210
AluBI AGCT 9 cut(s) 138, 167, 317, 694, 789, 947, 1120, 1153, 1158
AluI AGCT 9 cut(s) 138, 167, 317, 694, 789, 947, 1120, 1153, 1158
Alw21I GWGCWC 1 cut(s) 475
Alw26I GTCTC 5 cut(s) 25, 326, 599, 1290, 1297
AlwI GGATC 2 cut(s) 79, 92
Ama87I CYCGRG 1 cut(s) 94
Aor13HI TCCGGA 1 cut(s) 1249
AoxI GGCC 1 cut(s) 18
ApeKI GCWGC 1 cut(s) 913
ApoI RAATTY 4 cut(s) 385, 557, 829, 1051
AspLEI GCGC 2 cut(s) 868, 870
AspS9I GGNCC 2 cut(s) 280, 746
AsuC2I CCSGG 7 cut(s) 95, 96, 235, 260, 975, 1191, 1344
AsuHPI GGTGA 5 cut(s) 16, 539, 553, 1057, 1329
AvaI CYCGRG 1 cut(s) 94
AvaII GGWCC 2 cut(s) 280, 746
BamHI GGATCC 1 cut(s) 84
BanII GRGCYC 1 cut(s) 102
Bbv12I GWGCWC 1 cut(s) 475
BbvI GCAGC 1 cut(s) 925
BccI CCATC 7 cut(s) 68, 305, 575, 620, 828, 1003, 1061
BceAI ACGGC 1 cut(s) 774
BciT130I CCWGG 2 cut(s) 178, 1111
BcnI CCSGG 7 cut(s) 95, 96, 235, 260, 975, 1191, 1344
BcoDI GTCTC 5 cut(s) 25, 326, 599, 1290, 1297
BfaI CTAG 2 cut(s) 168, 1017
BfmI CTRYAG 3 cut(s) 501, 858, 948
BfrI CTTAAG 1 cut(s) 188
BisI GCNGC 1 cut(s) 914
BlpI GCTNAGC 1 cut(s) 1154
BlsI GCNGC 1 cut(s) 915
Bme1390I CCNGG 9 cut(s) 95, 96, 178, 235, 260, 975, 1111, 1191, 1344
Bme18I GGWCC 2 cut(s) 280, 746
BmeT110I CYCGRG 1 cut(s) 94
BmgT120I GGNCC 2 cut(s) 280, 746
BmiI GGNNCC 2 cut(s) 86, 101
BmrFI CCNGG 9 cut(s) 95, 96, 178, 235, 260, 975, 1111, 1191, 1344
BmsI GCATC 4 cut(s) 220, 311, 520, 798
BplI GAGNNNNNCTC 2 cut(s) 457, 489
BpmI CTGGAG 1 cut(s) 348
Bpu1102I GCTNAGC 1 cut(s) 1154
BpuEI CTTGAG 1 cut(s) 1153
BpuMI CCSGG 7 cut(s) 95, 96, 235, 260, 975, 1191, 1344
BsaI GGTCTC 3 cut(s) 25, 326, 1290
BsaJI CCNNGG 6 cut(s) 94, 95, 974, 1032, 1109, 1287
BsaWI WCCGGW 4 cut(s) 246, 282, 919, 1249
Bsc4I CCNNNNNNNGG 6 cut(s) 94, 183, 265, 343, 923, 1190
Bse1I ACTGG 3 cut(s) 331, 529, 564
Bse3DI GCAATG 2 cut(s) 440, 1336
BseAI TCCGGA 1 cut(s) 1249
BseBI CCWGG 2 cut(s) 178, 1111
BseDI CCNNGG 6 cut(s) 94, 95, 974, 1032, 1109, 1287
BseGI GGATG 8 cut(s) 60, 454, 631, 649, 813, 995, 1072, 1279
BseLI CCNNNNNNNGG 6 cut(s) 94, 183, 265, 343, 923, 1190
BseMI GCAATG 2 cut(s) 440, 1336
BseMII CTCAG 2 cut(s) 29, 1145
BseNI ACTGG 3 cut(s) 331, 529, 564
BsePI GCGCGC 1 cut(s) 866
BseXI GCAGC 1 cut(s) 925
Bsh1236I CGCG 2 cut(s) 868, 870
BshFI GGCC 1 cut(s) 20
BsiHKAI GWGCWC 1 cut(s) 475
BsiHKCI CYCGRG 1 cut(s) 94
BslI CCNNNNNNNGG 6 cut(s) 94, 183, 265, 343, 923, 1190
BsmAI GTCTC 5 cut(s) 25, 326, 599, 1290, 1297
BsmBI CGTCTC 1 cut(s) 1297
BsnI GGCC 1 cut(s) 20
Bso31I GGTCTC 3 cut(s) 25, 326, 1290
BsoBI CYCGRG 1 cut(s) 94
Bsp1286I GDGCHC 2 cut(s) 102, 475
Bsp13I TCCGGA 1 cut(s) 1249
Bsp1407I TGTACA 3 cut(s) 220, 895, 1161
Bsp143I GATC 1 cut(s) 84
Bsp1720I GCTNAGC 1 cut(s) 1154
Bsp19I CCATGG 2 cut(s) 1032, 1287
BspACI CCGC 2 cut(s) 1235, 1340
BspANI GGCC 1 cut(s) 20
BspCNI CTCAG 2 cut(s) 28, 1146
BspEI TCCGGA 1 cut(s) 1249
BspFNI CGCG 2 cut(s) 868, 870
BspLI GGNNCC 2 cut(s) 86, 101
BspMAI CTGCAG 1 cut(s) 505
BspPI GGATC 2 cut(s) 79, 92
BspTI CTTAAG 1 cut(s) 188
BspTNI GGTCTC 3 cut(s) 25, 326, 1290
BsrDI GCAATG 2 cut(s) 440, 1336
BsrGI TGTACA 3 cut(s) 220, 895, 1161
BsrI ACTGG 3 cut(s) 331, 529, 564
BssECI CCNNGG 6 cut(s) 94, 95, 974, 1032, 1109, 1287
BssHII GCGCGC 1 cut(s) 866
BssMI GATC 1 cut(s) 84
BssT1I CCWWGG 2 cut(s) 1032, 1287
Bst2UI CCWGG 2 cut(s) 178, 1111
Bst4CI ACNGT 3 cut(s) 211, 725, 1247
Bst6I CTCTTC 2 cut(s) 564, 1311
BstAFI CTTAAG 1 cut(s) 188
BstAPI GCANNNNNTGC 1 cut(s) 1013
BstAUI TGTACA 3 cut(s) 220, 895, 1161
BstC8I GCNNGC 2 cut(s) 597, 868
BstDEI CTNAG 2 cut(s) 15, 1154
BstDSI CCRYGG 2 cut(s) 1032, 1287
BstF5I GGATG 8 cut(s) 60, 454, 631, 649, 813, 995, 1072, 1279
BstFNI CGCG 2 cut(s) 868, 870
BstHHI GCGC 2 cut(s) 868, 870
BstKTI GATC 1 cut(s) 87
BstMAI GTCTC 5 cut(s) 25, 326, 599, 1290, 1297
BstMBI GATC 1 cut(s) 84
BstMWI GCNNNNNNNGC 7 cut(s) 48, 173, 656, 872, 913, 1013, 1232
BstNI CCWGG 2 cut(s) 178, 1111
BstSCI CCNGG 9 cut(s) 93, 94, 176, 233, 258, 973, 1109, 1189, 1342
BstSFI CTRYAG 3 cut(s) 501, 858, 948
BstUI CGCG 2 cut(s) 868, 870
BstV1I GCAGC 1 cut(s) 925
BstX2I RGATCY 1 cut(s) 84
BstYI RGATCY 1 cut(s) 84
BsuRI GGCC 1 cut(s) 20
BtgI CCRYGG 2 cut(s) 1032, 1287
BtsCI GGATG 8 cut(s) 60, 454, 631, 649, 813, 995, 1072, 1279
BtsIMutI CAGTG 3 cut(s) 571, 576, 730
Cac8I GCNNGC 2 cut(s) 597, 868
CfoI GCGC 2 cut(s) 868, 870
Cfr13I GGNCC 2 cut(s) 280, 746
Cfr9I CCCGGG 1 cut(s) 94
Csp6I GTAC 4 cut(s) 221, 896, 1162, 1222
CviAII CATG 3 cut(s) 1033, 1074, 1288
CviQI GTAC 4 cut(s) 221, 896, 1162, 1222
DdeI CTNAG 2 cut(s) 15, 1154
DpnI GATC 1 cut(s) 86
DpnII GATC 1 cut(s) 84
Eam1104I CTCTTC 2 cut(s) 564, 1311
EarI CTCTTC 2 cut(s) 564, 1311
Eco130I CCWWGG 2 cut(s) 1032, 1287
Eco24I GRGCYC 1 cut(s) 102
Eco31I GGTCTC 3 cut(s) 25, 326, 1290
Eco47I GGWCC 2 cut(s) 280, 746
Eco88I CYCGRG 1 cut(s) 94
EcoRI GAATTC 2 cut(s) 557, 829
EcoRII CCWGG 2 cut(s) 176, 1109
EcoT14I CCWWGG 2 cut(s) 1032, 1287
EcoT22I ATGCAT 1 cut(s) 535
EcoT38I GRGCYC 1 cut(s) 102
ErhI CCWWGG 2 cut(s) 1032, 1287
Esp3I CGTCTC 1 cut(s) 1297
FaeI CATG 3 cut(s) 1036, 1077, 1291
FalI AAGNNNNNCTT 2 cut(s) 1142, 1174
FatI CATG 3 cut(s) 1032, 1073, 1287
FblI GTMKAC 1 cut(s) 933
Fnu4HI GCNGC 1 cut(s) 914
FokI GGATG 8 cut(s) 47, 461, 638, 656, 820, 982, 1079, 1286
FriOI GRGCYC 1 cut(s) 102
Fsp4HI GCNGC 1 cut(s) 914
FspBI CTAG 2 cut(s) 168, 1017
GlaI GCGC 2 cut(s) 867, 869
GluI GCNGC 1 cut(s) 914
GsuI CTGGAG 1 cut(s) 348
HaeIII GGCC 1 cut(s) 20
HhaI GCGC 2 cut(s) 868, 870
Hin1II CATG 3 cut(s) 1036, 1077, 1291
Hin6I GCGC 2 cut(s) 866, 868
HinP1I GCGC 2 cut(s) 866, 868
HincII GTYRAC 1 cut(s) 721
HindII GTYRAC 1 cut(s) 721
HindIII AAGCTT 1 cut(s) 692
HphI GGTGA 5 cut(s) 16, 539, 553, 1057, 1329
Hpy166II GTNNAC 5 cut(s) 576, 721, 934, 1203, 1222
Hpy188I TCNGA 7 cut(s) 84, 198, 367, 682, 700, 731, 853
Hpy188III TCNNGA 1 cut(s) 1250
Hpy8I GTNNAC 5 cut(s) 576, 721, 934, 1203, 1222
HpyAV CCTTC 5 cut(s) 54, 334, 396, 917, 1247
HpyCH4III ACNGT 3 cut(s) 211, 725, 1247
HpyCH4V TGCA 5 cut(s) 78, 302, 503, 533, 907
HpyF10VI GCNNNNNNNGC 7 cut(s) 48, 173, 656, 872, 913, 1013, 1232
HpyF3I CTNAG 2 cut(s) 15, 1154
Hsp92II CATG 3 cut(s) 1036, 1077, 1291
HspAI GCGC 2 cut(s) 866, 868
Kpn2I TCCGGA 1 cut(s) 1249
Kzo9I GATC 1 cut(s) 84
LmnI GCTCC 3 cut(s) 56, 105, 314
Lsp1109I GCAGC 1 cut(s) 925
LweI GCATC 4 cut(s) 220, 311, 520, 798
MaeI CTAG 2 cut(s) 168, 1017
MaeIII GTNAC 4 cut(s) 4, 118, 205, 1019
MalI GATC 1 cut(s) 86
MboI GATC 1 cut(s) 84
MboII GAAGA 5 cut(s) 157, 563, 581, 1060, 1328
MfeI CAATTG 1 cut(s) 765
MflI RGATCY 1 cut(s) 84
MhlI GDGCHC 2 cut(s) 102, 475
MluCI AATT 9 cut(s) 160, 369, 385, 482, 508, 557, 765, 829, 1051
MlyI GAGTC 3 cut(s) 371, 1185, 1292
MmeI TCCRAC 4 cut(s) 154, 345, 1119, 1273
MnlI CCTC 8 cut(s) 83, 165, 367, 640, 759, 865, 981, 1128
Mph1103I ATGCAT 1 cut(s) 535
MroI TCCGGA 1 cut(s) 1249
MseI TTAA 3 cut(s) 159, 189, 1041
MslI CAYNNNNRTG 2 cut(s) 228, 624
MspCI CTTAAG 1 cut(s) 188
MspR9I CCNGG 9 cut(s) 95, 96, 178, 235, 260, 975, 1111, 1191, 1344
MunI CAATTG 1 cut(s) 765
MvaI CCWGG 2 cut(s) 178, 1111
MvnI CGCG 2 cut(s) 868, 870
MwoI GCNNNNNNNGC 7 cut(s) 48, 173, 656, 872, 913, 1013, 1232
NciI CCSGG 7 cut(s) 95, 96, 235, 260, 975, 1191, 1344
NcoI CCATGG 2 cut(s) 1032, 1287
NdeII GATC 1 cut(s) 84
NlaIII CATG 3 cut(s) 1036, 1077, 1291
NlaIV GGNNCC 2 cut(s) 86, 101
NmuCI GTSAC 3 cut(s) 4, 118, 1019
NsiI ATGCAT 1 cut(s) 535
PauI GCGCGC 1 cut(s) 866
PfeI GAWTC 7 cut(s) 193, 347, 497, 671, 713, 888, 1194
PflFI GACNNNGTC 1 cut(s) 725
PflMI CCANNNNNTGG 1 cut(s) 343
PkrI GCNGC 1 cut(s) 915
PleI GAGTC 3 cut(s) 370, 1184, 1291
PpsI GAGTC 3 cut(s) 370, 1184, 1291
PsiI TTATAA 1 cut(s) 755
Psp6I CCWGG 2 cut(s) 176, 1109
PspGI CCWGG 2 cut(s) 176, 1109
PspN4I GGNNCC 2 cut(s) 86, 101
PspPI GGNCC 2 cut(s) 280, 746
PstI CTGCAG 1 cut(s) 505
PsuI RGATCY 1 cut(s) 84
PsyI GACNNNGTC 1 cut(s) 725
PteI GCGCGC 1 cut(s) 866
RsaI GTAC 4 cut(s) 222, 897, 1163, 1223
RsaNI GTAC 4 cut(s) 221, 896, 1162, 1222
RseI CAYNNNNRTG 2 cut(s) 228, 624
SaqAI TTAA 3 cut(s) 159, 189, 1041
SatI GCNGC 1 cut(s) 914
Sau3AI GATC 1 cut(s) 84
Sau96I GGNCC 2 cut(s) 280, 746
SchI GAGTC 3 cut(s) 371, 1185, 1292
ScrFI CCNGG 9 cut(s) 95, 96, 178, 235, 260, 975, 1111, 1191, 1344
SduI GDGCHC 2 cut(s) 102, 475
SfaNI GCATC 4 cut(s) 220, 311, 520, 798
SfcI CTRYAG 3 cut(s) 501, 858, 948
SinI GGWCC 2 cut(s) 280, 746
SmaI CCCGGG 1 cut(s) 96
SmiMI CAYNNNNRTG 2 cut(s) 228, 624
SmlI CTYRAG 2 cut(s) 188, 1132
SmoI CTYRAG 2 cut(s) 188, 1132
Sse9I AATT 9 cut(s) 160, 369, 385, 482, 508, 557, 765, 829, 1051
SsiI CCGC 2 cut(s) 1235, 1340
SspMI CTAG 2 cut(s) 168, 1017
StyD4I CCNGG 9 cut(s) 93, 94, 176, 233, 258, 973, 1109, 1189, 1342
StyI CCWWGG 2 cut(s) 1032, 1287
TaaI ACNGT 3 cut(s) 211, 725, 1247
TaqI TCGA 3 cut(s) 255, 521, 1281
TasI AATT 9 cut(s) 160, 369, 385, 482, 508, 557, 765, 829, 1051
TatI WGTACW 3 cut(s) 220, 895, 1161
TfiI GAWTC 7 cut(s) 193, 347, 497, 671, 713, 888, 1194
Tru1I TTAA 3 cut(s) 159, 189, 1041
Tru9I TTAA 3 cut(s) 159, 189, 1041
TscAI CASTG 3 cut(s) 571, 583, 730
TseFI GTSAC 3 cut(s) 4, 118, 1019
TseI GCWGC 1 cut(s) 913
Tsp45I GTSAC 3 cut(s) 4, 118, 1019
TspDTI ATGAA 5 cut(s) 378, 570, 699, 957, 1182
TspGWI ACGGA 1 cut(s) 719
TspMI CCCGGG 1 cut(s) 94
TspRI CASTG 3 cut(s) 571, 583, 730
Tth111I GACNNNGTC 1 cut(s) 725
Van91I CCANNNNNTGG 1 cut(s) 343
Vha464I CTTAAG 1 cut(s) 188
VpaK11BI GGWCC 2 cut(s) 280, 746
XapI RAATTY 4 cut(s) 385, 557, 829, 1051
XmaI CCCGGG 1 cut(s) 94
XmiI GTMKAC 1 cut(s) 933
XspI CTAG 2 cut(s) 168, 1017
Zsp2I ATGCAT 1 cut(s) 535
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.