MD03G1204100.v1.1

DTW

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0012344
Physical Location & Seq
Reverse (-)
27867777 .. 27868280
504 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1204100.v1.1.491

Sequence Viewer

Length: 504 bp
ATGGTGACTACTCAGCCCGCTTCCAAACGACCTATATGCCAGTCGTGCTCCAAACCGGTCCGGCTCTGCCTCTGCACTCGGATTGAGAGCCCTGGGCTCCATAACTCTGTAAACGTCACCATTCTTCAACACAGCCTAGAGAAGAACCACCCACTTAATTCCGCTAGGATTGCTACATTAGGACTCAAAAATCTCAGTGTAGTTACTGTTTATGATGTTAGTTTTGAATCCCGGTTCGTTATCCGGTCGCCGGGCCCGGTTTCTCAAATGGGTTCGGACCGAAATGGTTTGAAAAGTTCAGGGTTTGATCAGCTTGTAGAAAGTAGAGAGACCCAGAAGCTGGTTTTTGTGTATTGTGATGAGGTGAGCGCAGGGAAAGAAAATGTGGGTAAGTTGCGTTTTGGGTTATCTGCAAATTTGGCAAAAGAGAGAAACAATAGTAGTTTGATTGATGGTGAGAAATGCAATTTTGATGGTGTTGGAGAACAATTCAAAAATAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DTW

Protein Analysis

168

Amino Acids

18.33

Weight (kDa)

9.11

Isoelectric Point (pI)

45.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DTW PF03942 10 - 73 1.2e-10 DTW domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012344)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G54880
fragaria_vesca FvH4_3g22471
malus_domestica MD03G1204100.v1.1 MD11G1220900.v1.1
prunus_persica Prupe.4G211300_v2.0.a1
pyrus_communis pycom03g15470 pycom11g19490
rosa_chinensis RchiOBHm_Chr5g0039141
rosa_laevigata RLG00000033897
rosa_multiflora Rmu_sc0000533.1_g000008 Rmu_ssc0000410.1_g000018 Rmu_ssc0000410.1_g000044
rosa_roxburghii Rroxscaffold_1G00040360
rosa_rugosa Rorug05G0177000
rosa_samantha Rh5BG268300 Rh5CG301100 Rh5DG276100
rosa_wichuraiana Rw0G001970 Rw5G024630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 340
AciI CCGC 2 cut(s) 18, 162
AcsI RAATTY 1 cut(s) 415
AfiI CCNNNNNNNGG 2 cut(s) 250, 340
AgeI ACCGGT 1 cut(s) 55
AgsI TTSAA 4 cut(s) 128, 227, 292, 493
AjnI CCWGG 1 cut(s) 91
AluBI AGCT 2 cut(s) 313, 340
AluI AGCT 2 cut(s) 313, 340
Alw21I GWGCWC 1 cut(s) 50
Alw26I GTCTC 1 cut(s) 323
AlwNI CAGNNNCTG 1 cut(s) 340
AoxI GGCC 1 cut(s) 253
ApaI GGGCCC 1 cut(s) 257
ApoI RAATTY 1 cut(s) 415
AsiGI ACCGGT 1 cut(s) 55
AspLEI GCGC 1 cut(s) 371
AspS9I GGNCC 4 cut(s) 58, 253, 254, 277
AsuC2I CCSGG 3 cut(s) 232, 252, 257
AsuHPI GGTGA 4 cut(s) 16, 109, 376, 467
AvaII GGWCC 2 cut(s) 58, 277
BaeGI GKGCMC 1 cut(s) 257
BanII GRGCYC 3 cut(s) 92, 99, 257
Bbv12I GWGCWC 1 cut(s) 50
BccI CCATC 2 cut(s) 446, 467
BcgI CGANNNNNNTGC 2 cut(s) 18, 52
BciT130I CCWGG 1 cut(s) 93
BclI TGATCA 1 cut(s) 307
BcnI CCSGG 3 cut(s) 232, 252, 257
BcoDI GTCTC 1 cut(s) 323
BfaI CTAG 2 cut(s) 137, 165
Bme1390I CCNGG 4 cut(s) 93, 232, 252, 257
Bme18I GGWCC 2 cut(s) 58, 277
BmgT120I GGNCC 4 cut(s) 58, 253, 254, 277
BmiI GGNNCC 2 cut(s) 98, 255
BmrFI CCNGG 4 cut(s) 93, 232, 252, 257
BpuMI CCSGG 3 cut(s) 232, 252, 257
BsaI GGTCTC 1 cut(s) 323
BsaJI CCNNGG 2 cut(s) 91, 92
BsaWI WCCGGW 2 cut(s) 55, 243
Bsc4I CCNNNNNNNGG 2 cut(s) 250, 340
Bse118I RCCGGY 1 cut(s) 55
Bse1I ACTGG 1 cut(s) 40
BseBI CCWGG 1 cut(s) 93
BseDI CCNNGG 2 cut(s) 91, 92
BseLI CCNNNNNNNGG 2 cut(s) 250, 340
BseMII CTCAG 2 cut(s) 26, 208
BseNI ACTGG 1 cut(s) 40
BseSI GKGCMC 1 cut(s) 257
BsgI GTGCAG 1 cut(s) 58
Bsh1285I CGRYCG 1 cut(s) 248
BshFI GGCC 1 cut(s) 255
BshTI ACCGGT 1 cut(s) 55
BsiEI CGRYCG 1 cut(s) 248
BsiHKAI GWGCWC 1 cut(s) 50
BsiSI CCGG 6 cut(s) 56, 61, 232, 244, 251, 257
BslI CCNNNNNNNGG 2 cut(s) 250, 340
BsmAI GTCTC 1 cut(s) 323
BsnI GGCC 1 cut(s) 255
Bso31I GGTCTC 1 cut(s) 323
Bsp120I GGGCCC 1 cut(s) 253
Bsp1286I GDGCHC 4 cut(s) 50, 92, 99, 257
Bsp143I GATC 1 cut(s) 307
BspACI CCGC 2 cut(s) 18, 162
BspANI GGCC 1 cut(s) 255
BspCNI CTCAG 2 cut(s) 25, 207
BspLI GGNNCC 2 cut(s) 98, 255
BspTNI GGTCTC 1 cut(s) 323
BsrFI RCCGGY 1 cut(s) 55
BsrI ACTGG 1 cut(s) 40
BssAI RCCGGY 1 cut(s) 55
BssECI CCNNGG 2 cut(s) 91, 92
BssMI GATC 1 cut(s) 307
Bst2UI CCWGG 1 cut(s) 93
Bst4CI ACNGT 1 cut(s) 208
BstC8I GCNNGC 1 cut(s) 18
BstDEI CTNAG 2 cut(s) 12, 194
BstHHI GCGC 1 cut(s) 371
BstKTI GATC 1 cut(s) 310
BstMAI GTCTC 1 cut(s) 323
BstMBI GATC 1 cut(s) 307
BstMCI CGRYCG 1 cut(s) 248
BstMWI GCNNNNNNNGC 3 cut(s) 45, 170, 419
BstNI CCWGG 1 cut(s) 93
BstSCI CCNGG 4 cut(s) 91, 230, 250, 255
BstSLI GKGCMC 1 cut(s) 257
BsuRI GGCC 1 cut(s) 255
BtsIMutI CAGTG 1 cut(s) 202
Cac8I GCNNGC 1 cut(s) 18
CaiI CAGNNNCTG 1 cut(s) 340
CfoI GCGC 1 cut(s) 371
Cfr10I RCCGGY 1 cut(s) 55
Cfr13I GGNCC 4 cut(s) 58, 253, 254, 277
CpoI CGGWCCG 2 cut(s) 58, 277
CspAI ACCGGT 1 cut(s) 55
CspI CGGWCCG 2 cut(s) 58, 277
CviJI RGCY 8 cut(s) 16, 64, 90, 97, 135, 255, 313, 340
CviKI_1 RGCY 8 cut(s) 16, 64, 90, 97, 135, 255, 313, 340
DdeI CTNAG 2 cut(s) 12, 194
DpnI GATC 1 cut(s) 309
DpnII GATC 1 cut(s) 307
Eco24I GRGCYC 3 cut(s) 92, 99, 257
Eco31I GGTCTC 1 cut(s) 323
Eco47I GGWCC 2 cut(s) 58, 277
EcoRII CCWGG 1 cut(s) 91
EcoT38I GRGCYC 3 cut(s) 92, 99, 257
FaiI YATR 4 cut(s) 35, 37, 102, 213
FauI CCCGC 1 cut(s) 25
FbaI TGATCA 1 cut(s) 307
FriOI GRGCYC 3 cut(s) 92, 99, 257
FspBI CTAG 2 cut(s) 137, 165
GlaI GCGC 1 cut(s) 370
HaeIII GGCC 1 cut(s) 255
HapII CCGG 6 cut(s) 56, 61, 232, 244, 251, 257
HhaI GCGC 1 cut(s) 371
Hin6I GCGC 1 cut(s) 369
HinP1I GCGC 1 cut(s) 369
HinfI GANTC 2 cut(s) 183, 227
HpaII CCGG 6 cut(s) 56, 61, 232, 244, 251, 257
HphI GGTGA 4 cut(s) 16, 109, 376, 467
Hpy166II GTNNAC 1 cut(s) 112
Hpy188I TCNGA 2 cut(s) 81, 277
Hpy8I GTNNAC 1 cut(s) 112
HpyCH4III ACNGT 1 cut(s) 208
HpyCH4IV ACGT 1 cut(s) 114
HpyCH4V TGCA 3 cut(s) 75, 413, 465
HpyF10VI GCNNNNNNNGC 3 cut(s) 45, 170, 419
HpyF3I CTNAG 2 cut(s) 12, 194
HpySE526I ACGT 1 cut(s) 114
HspAI GCGC 1 cut(s) 369
Ksp22I TGATCA 1 cut(s) 307
Kzo9I GATC 1 cut(s) 307
LmnI GCTCC 2 cut(s) 53, 102
MaeI CTAG 2 cut(s) 137, 165
MaeII ACGT 1 cut(s) 114
MaeIII GTNAC 3 cut(s) 4, 115, 202
MalI GATC 1 cut(s) 309
MboI GATC 1 cut(s) 307
MboII GAAGA 2 cut(s) 116, 154
MhlI GDGCHC 4 cut(s) 50, 92, 99, 257
MluCI AATT 4 cut(s) 157, 415, 466, 488
MlyI GAGTC 1 cut(s) 177
MmeI TCCRAC 1 cut(s) 460
MnlI CCTC 2 cut(s) 80, 355
MseI TTAA 1 cut(s) 156
MspI CCGG 6 cut(s) 56, 61, 232, 244, 251, 257
MspR9I CCNGG 4 cut(s) 93, 232, 252, 257
MvaI CCWGG 1 cut(s) 93
MwoI GCNNNNNNNGC 3 cut(s) 45, 170, 419
NciI CCSGG 3 cut(s) 232, 252, 257
NdeII GATC 1 cut(s) 307
NlaIV GGNNCC 2 cut(s) 98, 255
NmuCI GTSAC 2 cut(s) 4, 115
PasI CCCWGGG 1 cut(s) 92
PfeI GAWTC 1 cut(s) 227
PflMI CCANNNNNTGG 1 cut(s) 340
PinAI ACCGGT 1 cut(s) 55
PleI GAGTC 1 cut(s) 177
PpsI GAGTC 1 cut(s) 177
Psp6I CCWGG 1 cut(s) 91
PspGI CCWGG 1 cut(s) 91
PspN4I GGNNCC 2 cut(s) 98, 255
PspOMI GGGCCC 1 cut(s) 253
PspPI GGNCC 4 cut(s) 58, 253, 254, 277
PstNI CAGNNNCTG 1 cut(s) 340
Rsr2I CGGWCCG 2 cut(s) 58, 277
RsrII CGGWCCG 2 cut(s) 58, 277
SaqAI TTAA 1 cut(s) 156
Sau3AI GATC 1 cut(s) 307
Sau96I GGNCC 4 cut(s) 58, 253, 254, 277
SchI GAGTC 1 cut(s) 177
ScrFI CCNGG 4 cut(s) 93, 232, 252, 257
SduI GDGCHC 4 cut(s) 50, 92, 99, 257
SetI ASST 5 cut(s) 34, 117, 315, 342, 366
SinI GGWCC 2 cut(s) 58, 277
Sse9I AATT 4 cut(s) 157, 415, 466, 488
SsiI CCGC 2 cut(s) 18, 162
SspMI CTAG 2 cut(s) 137, 165
StyD4I CCNGG 4 cut(s) 91, 230, 250, 255
TaaI ACNGT 1 cut(s) 208
TaiI ACGT 1 cut(s) 117
TaqII GACCGA 1 cut(s) 294
TasI AATT 4 cut(s) 157, 415, 466, 488
TfiI GAWTC 1 cut(s) 227
Tru1I TTAA 1 cut(s) 156
Tru9I TTAA 1 cut(s) 156
TscAI CASTG 1 cut(s) 202
TseFI GTSAC 2 cut(s) 4, 115
Tsp45I GTSAC 2 cut(s) 4, 115
TspRI CASTG 1 cut(s) 202
Van91I CCANNNNNTGG 1 cut(s) 340
VpaK11BI GGWCC 2 cut(s) 58, 277
XapI RAATTY 1 cut(s) 415
XspI CTAG 2 cut(s) 137, 165
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.