pycom03g15470

DTW

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0012344
Physical Location & Seq
Reverse (-)
16720205 .. 16720684
480 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g15470.1

Sequence Viewer

Length: 414 bp
ATGGTGACTACTCAGCCCGCTTCCAAACGACCCATATGCCCATTGTGCTCCAAACCGGTCCGGCTCTGCCTCTGCACTCGGATTCAGAACCCGGGGCTCCATAACTCTGTAAGCGTCACCATTCTTCAACACAGCCTAGAGAAGAACCACCCACTTAATTCCGTTAGGATTGCTAGATTAGGACTCAAAAATATCAGTTTAGTTACTGTTTATGATGTTAATTTTGAATCCCGGTTCGTTATCCTGTCGTCGGGCCCCGTTTCTCAGATGGGTTCGGTCCGCAATGATTTGAAAAGTTCAGGCGCAGGGAAGGGAAATATGGGTAACTTGGGATTTGGGTTATCTACAAATTTGGCAAAAGAGAAAAACAATAGTAGTTGGATTGATAGAAATGCAATTTTGGTAGTGATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DTW

Protein Analysis

138

Amino Acids

14.84

Weight (kDa)

10.13

Isoelectric Point (pI)

37.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DTW PF03942 10 - 73 1e-09 DTW domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012344)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G54880
fragaria_vesca FvH4_3g22471
malus_domestica MD03G1204100.v1.1 MD11G1220900.v1.1
prunus_persica Prupe.4G211300_v2.0.a1
pyrus_communis pycom03g15470 pycom11g19490
rosa_chinensis RchiOBHm_Chr5g0039141
rosa_laevigata RLG00000033897
rosa_multiflora Rmu_sc0000533.1_g000008 Rmu_ssc0000410.1_g000018 Rmu_ssc0000410.1_g000044
rosa_roxburghii Rroxscaffold_1G00040360
rosa_rugosa Rorug05G0177000
rosa_samantha Rh5BG268300 Rh5CG301100 Rh5DG276100
rosa_wichuraiana Rw0G001970 Rw5G024630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 18, 280
AcsI RAATTY 1 cut(s) 349
AfiI CCNNNNNNNGG 1 cut(s) 250
AgeI ACCGGT 1 cut(s) 55
AgsI TTSAA 3 cut(s) 128, 227, 292
Alw21I GWGCWC 1 cut(s) 50
Ama87I CYCGRG 1 cut(s) 91
AoxI GGCC 1 cut(s) 253
ApaI GGGCCC 1 cut(s) 257
ApoI RAATTY 1 cut(s) 349
AsiGI ACCGGT 1 cut(s) 55
AspLEI GCGC 1 cut(s) 305
AspS9I GGNCC 4 cut(s) 58, 253, 254, 277
AsuC2I CCSGG 3 cut(s) 92, 93, 232
AsuHPI GGTGA 2 cut(s) 16, 109
AvaI CYCGRG 1 cut(s) 91
AvaII GGWCC 2 cut(s) 58, 277
BaeGI GKGCMC 1 cut(s) 257
BanII GRGCYC 2 cut(s) 99, 257
Bbv12I GWGCWC 1 cut(s) 50
BccI CCATC 1 cut(s) 262
BcgI CGANNNNNNTGC 2 cut(s) 18, 52
BcnI CCSGG 3 cut(s) 92, 93, 232
BfaI CTAG 2 cut(s) 137, 174
Bme1390I CCNGG 3 cut(s) 92, 93, 232
Bme18I GGWCC 2 cut(s) 58, 277
BmeT110I CYCGRG 1 cut(s) 91
BmgT120I GGNCC 4 cut(s) 58, 253, 254, 277
BmiI GGNNCC 3 cut(s) 98, 255, 256
BmrFI CCNGG 3 cut(s) 92, 93, 232
BpuMI CCSGG 3 cut(s) 92, 93, 232
BsaJI CCNNGG 2 cut(s) 91, 92
BsaWI WCCGGW 1 cut(s) 55
Bsc4I CCNNNNNNNGG 1 cut(s) 250
Bse118I RCCGGY 1 cut(s) 55
Bse3DI GCAATG 1 cut(s) 289
BseDI CCNNGG 2 cut(s) 91, 92
BseLI CCNNNNNNNGG 1 cut(s) 250
BseMI GCAATG 1 cut(s) 289
BseMII CTCAG 2 cut(s) 26, 278
BseSI GKGCMC 1 cut(s) 257
BsgI GTGCAG 1 cut(s) 58
BshFI GGCC 1 cut(s) 255
BshTI ACCGGT 1 cut(s) 55
BsiHKAI GWGCWC 1 cut(s) 50
BsiHKCI CYCGRG 1 cut(s) 91
BsiSI CCGG 4 cut(s) 56, 61, 92, 232
BslI CCNNNNNNNGG 1 cut(s) 250
BsnI GGCC 1 cut(s) 255
BsoBI CYCGRG 1 cut(s) 91
Bsp120I GGGCCC 1 cut(s) 253
Bsp1286I GDGCHC 3 cut(s) 50, 99, 257
BspACI CCGC 2 cut(s) 18, 280
BspANI GGCC 1 cut(s) 255
BspCNI CTCAG 2 cut(s) 25, 277
BspLI GGNNCC 3 cut(s) 98, 255, 256
BsrDI GCAATG 1 cut(s) 289
BsrFI RCCGGY 1 cut(s) 55
BssAI RCCGGY 1 cut(s) 55
BssECI CCNNGG 2 cut(s) 91, 92
Bst4CI ACNGT 1 cut(s) 208
BstC8I GCNNGC 1 cut(s) 18
BstDEI CTNAG 2 cut(s) 12, 264
BstHHI GCGC 1 cut(s) 305
BstMWI GCNNNNNNNGC 1 cut(s) 45
BstSCI CCNGG 3 cut(s) 90, 91, 230
BstSLI GKGCMC 1 cut(s) 257
BsuRI GGCC 1 cut(s) 255
Cac8I GCNNGC 1 cut(s) 18
CfoI GCGC 1 cut(s) 305
Cfr10I RCCGGY 1 cut(s) 55
Cfr13I GGNCC 4 cut(s) 58, 253, 254, 277
Cfr9I CCCGGG 1 cut(s) 91
CpoI CGGWCCG 2 cut(s) 58, 277
CseI GACGC 1 cut(s) 103
CspAI ACCGGT 1 cut(s) 55
CspI CGGWCCG 2 cut(s) 58, 277
CviJI RGCY 5 cut(s) 16, 64, 97, 135, 255
CviKI_1 RGCY 5 cut(s) 16, 64, 97, 135, 255
DdeI CTNAG 2 cut(s) 12, 264
Eco24I GRGCYC 2 cut(s) 99, 257
Eco47I GGWCC 2 cut(s) 58, 277
Eco88I CYCGRG 1 cut(s) 91
EcoO109I RGGNCCY 1 cut(s) 254
EcoT38I GRGCYC 2 cut(s) 99, 257
FaiI YATR 5 cut(s) 35, 37, 102, 213, 320
FauI CCCGC 1 cut(s) 25
FauNDI CATATG 1 cut(s) 35
FriOI GRGCYC 2 cut(s) 99, 257
FspBI CTAG 2 cut(s) 137, 174
GlaI GCGC 1 cut(s) 304
HaeIII GGCC 1 cut(s) 255
HapII CCGG 4 cut(s) 56, 61, 92, 232
HgaI GACGC 1 cut(s) 103
HhaI GCGC 1 cut(s) 305
Hin6I GCGC 1 cut(s) 303
HinP1I GCGC 1 cut(s) 303
HinfI GANTC 3 cut(s) 82, 183, 227
HpaII CCGG 4 cut(s) 56, 61, 92, 232
HphI GGTGA 2 cut(s) 16, 109
Hpy188I TCNGA 3 cut(s) 81, 87, 267
Hpy99I CGWCG 1 cut(s) 253
HpyAV CCTTC 1 cut(s) 304
HpyCH4III ACNGT 1 cut(s) 208
HpyCH4V TGCA 2 cut(s) 75, 395
HpyF10VI GCNNNNNNNGC 1 cut(s) 45
HpyF3I CTNAG 2 cut(s) 12, 264
HspAI GCGC 1 cut(s) 303
LmnI GCTCC 2 cut(s) 53, 102
LpnPI CCDG 7 cut(s) 69, 74, 105, 245, 257, 285, 291
MaeI CTAG 2 cut(s) 137, 174
MaeIII GTNAC 4 cut(s) 4, 115, 202, 323
MboII GAAGA 2 cut(s) 116, 154
MhlI GDGCHC 3 cut(s) 50, 99, 257
MluCI AATT 4 cut(s) 157, 220, 349, 396
MlyI GAGTC 1 cut(s) 177
MmeI TCCRAC 1 cut(s) 359
MnlI CCTC 1 cut(s) 80
MseI TTAA 2 cut(s) 156, 219
MspI CCGG 4 cut(s) 56, 61, 92, 232
MspR9I CCNGG 3 cut(s) 92, 93, 232
MwoI GCNNNNNNNGC 1 cut(s) 45
NciI CCSGG 3 cut(s) 92, 93, 232
NdeI CATATG 1 cut(s) 35
NlaIV GGNNCC 3 cut(s) 98, 255, 256
NmuCI GTSAC 2 cut(s) 4, 115
PfeI GAWTC 2 cut(s) 82, 227
PinAI ACCGGT 1 cut(s) 55
PleI GAGTC 1 cut(s) 177
PpsI GAGTC 1 cut(s) 177
PspN4I GGNNCC 3 cut(s) 98, 255, 256
PspOMI GGGCCC 1 cut(s) 253
PspPI GGNCC 4 cut(s) 58, 253, 254, 277
Rsr2I CGGWCCG 2 cut(s) 58, 277
RsrII CGGWCCG 2 cut(s) 58, 277
SaqAI TTAA 2 cut(s) 156, 219
Sau96I GGNCC 4 cut(s) 58, 253, 254, 277
SchI GAGTC 1 cut(s) 177
ScrFI CCNGG 3 cut(s) 92, 93, 232
SduI GDGCHC 3 cut(s) 50, 99, 257
SinI GGWCC 2 cut(s) 58, 277
SmaI CCCGGG 1 cut(s) 93
Sse9I AATT 4 cut(s) 157, 220, 349, 396
SsiI CCGC 2 cut(s) 18, 280
SspMI CTAG 2 cut(s) 137, 174
StyD4I CCNGG 3 cut(s) 90, 91, 230
TaaI ACNGT 1 cut(s) 208
TaqII GACCGA 1 cut(s) 265
TasI AATT 4 cut(s) 157, 220, 349, 396
TfiI GAWTC 2 cut(s) 82, 227
Tru1I TTAA 2 cut(s) 156, 219
Tru9I TTAA 2 cut(s) 156, 219
TseFI GTSAC 2 cut(s) 4, 115
Tsp45I GTSAC 2 cut(s) 4, 115
TspGWI ACGGA 1 cut(s) 151
TspMI CCCGGG 1 cut(s) 91
VpaK11BI GGWCC 2 cut(s) 58, 277
XapI RAATTY 1 cut(s) 349
XmaI CCCGGG 1 cut(s) 91
XspI CTAG 2 cut(s) 137, 174
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.