FvH4_3g28390

Belongs to the glycosyl hydrolase 17 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0008128
Physical Location & Seq
Forward (+)
21348320 .. 21349336
1017 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g28390.t1

Sequence Viewer

Length: 852 bp
ATGAGGATTTATGAGCTAAACCAAGCAACCTTGGAAGCCCTTAGAGGTTCCAACATAGAACTCATGGTCACCATCCTCAACAACAACCTGCAAGCACTTACTGATTCTGCAGCAGCTACAGATTGGGTCCAGAAAAATGTGCAACCCTACTCGACTGATGTCAAGCTCAAATACATTGCCATTAGAAATGAAGTACACCCTGATGCTGTAGAAGCTCAATATCTTCTTCCTGCCATACAGAATATTCAGAATGCGGTAACAGCCGCTAATCAACAAGGCCAAATCAAAGTGTCAACAGCAATAGATACAACTCTTTTGGACCCTAGTTTCCCTCATTCAGATGGGGCATTCAGCAGTGCTACGAATTCATTCATTACCCTAATAATCACCTTTTTGGGCAACAATGGAGCACCCCTTCTTGTCAATATATATCCCTACTTCGCCTATATTGGTGACCATGCTAACATAAAACTAGAATATGCCTTGTTCACTTCACCAGGGGTAGTAGTACAGAATGGTAGTAATGGGTACCAGAACATCTTTGATGCTATCTTGGACACTCATTACTCTGCTCTTGAAAAAGCCAAGGCTCCCAATATGGCGATAGTTGTATCCGAGAGTGGTTGGCCATCTGAAGGTAGTGATGCTGCAACTAATGGTAATGCAAGAACTTACTACAGCAATTTGATCGATCATGTGAAGACCGGGACTCCAAAAAGACCAAATGGAGCTATCGAAAGTTATCTATTTGCCATGTTTGATGAAAACCTCAAGGACGGTGCAGAAGTTGAGAAATATTTTGGTATCTTCTCCCCTAACAAACAGCCCAAGTACCAACTCACCTTTGGCTAG

Protein Analysis

284

Amino Acids

30.98

Weight (kDa)

4.98

Isoelectric Point (pI)

34.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_17 PF00332 1 - 282 1.1e-87 Glycosyl hydrolases family 17
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 96
Acc65I GGTACC 1 cut(s) 528
AccB1I GGYRCC 1 cut(s) 528
AciI CCGC 2 cut(s) 254, 264
AcoI YGGCCR 1 cut(s) 626
AcsI RAATTY 1 cut(s) 364
AcuI CTGAAG 1 cut(s) 654
AfaI GTAC 4 cut(s) 195, 510, 530, 833
AfiI CCNNNNNNNGG 1 cut(s) 635
AgsI TTSAA 1 cut(s) 578
AjnI CCWGG 1 cut(s) 496
AluBI AGCT 5 cut(s) 16, 116, 166, 215, 731
AluI AGCT 5 cut(s) 16, 116, 166, 215, 731
Alw21I GWGCWC 1 cut(s) 412
AoxI GGCC 2 cut(s) 277, 626
ApeKI GCWGC 3 cut(s) 110, 113, 647
ApoI RAATTY 1 cut(s) 364
Asp700I GAANNNNTTC 1 cut(s) 368
Asp718I GGTACC 1 cut(s) 528
AspS9I GGNCC 2 cut(s) 127, 319
AsuC2I CCSGG 1 cut(s) 706
AsuHPI GGTGA 5 cut(s) 61, 379, 464, 486, 832
AvaII GGWCC 2 cut(s) 127, 319
BalI TGGCCA 1 cut(s) 628
BanI GGYRCC 1 cut(s) 528
BbsI GAAGAC 1 cut(s) 707
Bbv12I GWGCWC 1 cut(s) 412
BbvI GCAGC 3 cut(s) 122, 125, 634
BccI CCATC 3 cut(s) 80, 335, 637
BcgI CGANNNNNNTGC 2 cut(s) 670, 704
BciT130I CCWGG 1 cut(s) 498
BciVI GTATCC 1 cut(s) 622
BcnI CCSGG 1 cut(s) 706
BfaI CTAG 3 cut(s) 324, 473, 850
BfmI CTRYAG 4 cut(s) 108, 117, 207, 676
BfuAI ACCTGC 1 cut(s) 96
BfuI GTATCC 1 cut(s) 622
BisI GCNGC 4 cut(s) 111, 114, 264, 648
BlsI GCNGC 4 cut(s) 112, 115, 265, 649
Bme1390I CCNGG 2 cut(s) 498, 706
Bme18I GGWCC 2 cut(s) 127, 319
BmgT120I GGNCC 2 cut(s) 127, 319
BmiI GGNNCC 5 cut(s) 49, 128, 321, 530, 591
BmrFI CCNGG 2 cut(s) 498, 706
BmsI GCATC 3 cut(s) 193, 535, 634
BoxI GACNNNNGTC 1 cut(s) 158
BpiI GAAGAC 1 cut(s) 707
BpuEI CTTGAG 1 cut(s) 755
BpuMI CCSGG 1 cut(s) 706
Bsa29I ATCGAT 1 cut(s) 690
BsaJI CCNNGG 3 cut(s) 30, 497, 585
BsaXI ACNNNNNCTCC 2 cut(s) 694, 724
Bsc4I CCNNNNNNNGG 1 cut(s) 635
Bse3DI GCAATG 1 cut(s) 174
BseBI CCWGG 1 cut(s) 498
BseCI ATCGAT 1 cut(s) 690
BseDI CCNNGG 3 cut(s) 30, 497, 585
BseGI GGATG 1 cut(s) 72
BseLI CCNNNNNNNGG 1 cut(s) 635
BseMI GCAATG 1 cut(s) 174
BseXI GCAGC 3 cut(s) 122, 125, 634
BsgI GTGCAG 1 cut(s) 801
BshFI GGCC 2 cut(s) 279, 628
BshNI GGYRCC 1 cut(s) 528
BshVI ATCGAT 1 cut(s) 690
BsiHKAI GWGCWC 1 cut(s) 412
BsiSI CCGG 1 cut(s) 705
BslFI GGGAC 1 cut(s) 721
BslI CCNNNNNNNGG 1 cut(s) 635
BsmFI GGGAC 1 cut(s) 721
BsmI GAATGC 2 cut(s) 256, 347
BsnI GGCC 2 cut(s) 279, 628
Bsp1286I GDGCHC 1 cut(s) 412
Bsp143I GATC 2 cut(s) 687, 691
BspACI CCGC 2 cut(s) 254, 264
BspANI GGCC 2 cut(s) 279, 628
BspDI ATCGAT 1 cut(s) 690
BspLI GGNNCC 5 cut(s) 49, 128, 321, 530, 591
BspMAI CTGCAG 1 cut(s) 112
BspMI ACCTGC 1 cut(s) 96
BspT107I GGYRCC 1 cut(s) 528
BsrDI GCAATG 1 cut(s) 174
BssECI CCNNGG 3 cut(s) 30, 497, 585
BssMI GATC 2 cut(s) 687, 691
BssT1I CCWWGG 2 cut(s) 30, 585
Bst2UI CCWGG 1 cut(s) 498
Bst4CI ACNGT 1 cut(s) 779
BstC8I GCNNGC 1 cut(s) 93
BstDEI CTNAG 1 cut(s) 41
BstEII GGTNACC 2 cut(s) 67, 452
BstF5I GGATG 1 cut(s) 72
BstKTI GATC 2 cut(s) 690, 694
BstMBI GATC 2 cut(s) 687, 691
BstMWI GCNNNNNNNGC 2 cut(s) 212, 260
BstNI CCWGG 1 cut(s) 498
BstPAI GACNNNNGTC 1 cut(s) 158
BstPI GGTNACC 2 cut(s) 67, 452
BstSCI CCNGG 2 cut(s) 496, 704
BstSFI CTRYAG 4 cut(s) 108, 117, 207, 676
BstV1I GCAGC 3 cut(s) 122, 125, 634
BstV2I GAAGAC 1 cut(s) 707
Bsu15I ATCGAT 1 cut(s) 690
BsuI GTATCC 1 cut(s) 622
BsuRI GGCC 2 cut(s) 279, 628
BsuTUI ATCGAT 1 cut(s) 690
BtsCI GGATG 1 cut(s) 72
BtsI GCAGTG 1 cut(s) 361
BtsIMutI CAGTG 1 cut(s) 361
BveI ACCTGC 1 cut(s) 96
Cac8I GCNNGC 1 cut(s) 93
Cfr13I GGNCC 2 cut(s) 127, 319
ClaI ATCGAT 1 cut(s) 690
Csp6I GTAC 4 cut(s) 194, 509, 529, 832
CviAII CATG 4 cut(s) 64, 458, 695, 754
CviQI GTAC 4 cut(s) 194, 509, 529, 832
DdeI CTNAG 1 cut(s) 41
DpnI GATC 2 cut(s) 689, 693
DpnII GATC 2 cut(s) 687, 691
EaeI YGGCCR 1 cut(s) 626
Eco130I CCWWGG 2 cut(s) 30, 585
Eco47I GGWCC 2 cut(s) 127, 319
Eco57I CTGAAG 1 cut(s) 654
Eco91I GGTNACC 2 cut(s) 67, 452
EcoO65I GGTNACC 2 cut(s) 67, 452
EcoRI GAATTC 1 cut(s) 364
EcoRII CCWGG 1 cut(s) 496
EcoT14I CCWWGG 2 cut(s) 30, 585
ErhI CCWWGG 2 cut(s) 30, 585
FaeI CATG 4 cut(s) 67, 461, 698, 757
FaqI GGGAC 1 cut(s) 721
FatI CATG 4 cut(s) 63, 457, 694, 753
Fnu4HI GCNGC 4 cut(s) 111, 114, 264, 648
FokI GGATG 1 cut(s) 59
Fsp4HI GCNGC 4 cut(s) 111, 114, 264, 648
FspBI CTAG 3 cut(s) 324, 473, 850
GluI GCNGC 4 cut(s) 111, 114, 264, 648
HaeIII GGCC 2 cut(s) 279, 628
HapII CCGG 1 cut(s) 705
Hin1II CATG 4 cut(s) 67, 461, 698, 757
HincII GTYRAC 1 cut(s) 294
HindII GTYRAC 1 cut(s) 294
HinfI GANTC 2 cut(s) 104, 709
HpaII CCGG 1 cut(s) 705
HphI GGTGA 5 cut(s) 61, 379, 464, 486, 832
Hpy166II GTNNAC 3 cut(s) 196, 294, 489
Hpy188I TCNGA 4 cut(s) 249, 340, 616, 634
Hpy188III TCNNGA 2 cut(s) 130, 575
Hpy8I GTNNAC 3 cut(s) 196, 294, 489
HpyAV CCTTC 2 cut(s) 425, 629
HpyCH4III ACNGT 1 cut(s) 779
HpyCH4V TGCA 6 cut(s) 91, 110, 142, 650, 665, 782
HpyF10VI GCNNNNNNNGC 2 cut(s) 212, 260
HpyF3I CTNAG 1 cut(s) 41
Hsp92II CATG 4 cut(s) 67, 461, 698, 757
KpnI GGTACC 1 cut(s) 532
Kzo9I GATC 2 cut(s) 687, 691
LmnI GCTCC 3 cut(s) 407, 595, 728
LpnPI CCDG 8 cut(s) 101, 143, 213, 243, 483, 510, 545, 718
Lsp1109I GCAGC 3 cut(s) 122, 125, 634
LweI GCATC 3 cut(s) 193, 535, 634
MaeI CTAG 3 cut(s) 324, 473, 850
MaeIII GTNAC 3 cut(s) 67, 256, 452
MalI GATC 2 cut(s) 689, 693
MboI GATC 2 cut(s) 687, 691
MboII GAAGA 4 cut(s) 215, 218, 712, 799
MhlI GDGCHC 1 cut(s) 412
MlsI TGGCCA 1 cut(s) 628
MluCI AATT 2 cut(s) 364, 682
MluNI TGGCCA 1 cut(s) 628
MlyI GAGTC 1 cut(s) 703
MmeI TCCRAC 1 cut(s) 75
MnlI CCTC 4 cut(s) 38, 86, 342, 779
Mox20I TGGCCA 1 cut(s) 628
MroXI GAANNNNTTC 1 cut(s) 368
MscI TGGCCA 1 cut(s) 628
MslI CAYNNNNRTG 2 cut(s) 201, 339
Msp20I TGGCCA 1 cut(s) 628
MspI CCGG 1 cut(s) 705
MspR9I CCNGG 2 cut(s) 498, 706
Mva1269I GAATGC 2 cut(s) 256, 347
MvaI CCWGG 1 cut(s) 498
MwoI GCNNNNNNNGC 2 cut(s) 212, 260
NciI CCSGG 1 cut(s) 706
NdeII GATC 2 cut(s) 687, 691
NlaIII CATG 4 cut(s) 67, 461, 698, 757
NlaIV GGNNCC 5 cut(s) 49, 128, 321, 530, 591
NmuCI GTSAC 2 cut(s) 67, 452
PctI GAATGC 2 cut(s) 256, 347
PdmI GAANNNNTTC 1 cut(s) 368
PfeI GAWTC 1 cut(s) 104
PkrI GCNGC 4 cut(s) 112, 115, 265, 649
PleI GAGTC 1 cut(s) 703
PpsI GAGTC 1 cut(s) 703
PshAI GACNNNNGTC 1 cut(s) 158
Psp6I CCWGG 1 cut(s) 496
PspEI GGTNACC 2 cut(s) 67, 452
PspGI CCWGG 1 cut(s) 496
PspN4I GGNNCC 5 cut(s) 49, 128, 321, 530, 591
PspPI GGNCC 2 cut(s) 127, 319
PstI CTGCAG 1 cut(s) 112
RsaI GTAC 4 cut(s) 195, 510, 530, 833
RsaNI GTAC 4 cut(s) 194, 509, 529, 832
RseI CAYNNNNRTG 2 cut(s) 201, 339
SatI GCNGC 4 cut(s) 111, 114, 264, 648
Sau3AI GATC 2 cut(s) 687, 691
Sau96I GGNCC 2 cut(s) 127, 319
SchI GAGTC 1 cut(s) 703
ScrFI CCNGG 2 cut(s) 498, 706
SduI GDGCHC 1 cut(s) 412
SfaNI GCATC 3 cut(s) 193, 535, 634
SfcI CTRYAG 4 cut(s) 108, 117, 207, 676
SinI GGWCC 2 cut(s) 127, 319
SmiMI CAYNNNNRTG 2 cut(s) 201, 339
SmlI CTYRAG 1 cut(s) 770
SmoI CTYRAG 1 cut(s) 770
Sse9I AATT 2 cut(s) 364, 682
SsiI CCGC 2 cut(s) 254, 264
SspI AATATT 2 cut(s) 244, 797
SspMI CTAG 3 cut(s) 324, 473, 850
StyD4I CCNGG 2 cut(s) 496, 704
StyI CCWWGG 2 cut(s) 30, 585
TaaI ACNGT 1 cut(s) 779
TaqI TCGA 3 cut(s) 152, 690, 735
TasI AATT 2 cut(s) 364, 682
TatI WGTACW 2 cut(s) 193, 508
TauI GCSGC 1 cut(s) 266
TfiI GAWTC 1 cut(s) 104
TscAI CASTG 1 cut(s) 361
TseFI GTSAC 2 cut(s) 67, 452
TseI GCWGC 3 cut(s) 110, 113, 647
Tsp45I GTSAC 2 cut(s) 67, 452
TspDTI ATGAA 4 cut(s) 204, 357, 361, 777
TspRI CASTG 1 cut(s) 361
VpaK11BI GGWCC 2 cut(s) 127, 319
XapI RAATTY 1 cut(s) 364
XcmI CCANNNNNNNNNTGG 1 cut(s) 842
XmnI GAANNNNTTC 1 cut(s) 368
XspI CTAG 3 cut(s) 324, 473, 850
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.