pycom11g16540

Belongs to the glycosyl hydrolase 17 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0008128
Physical Location & Seq
Forward (+)
18172534 .. 18174429
1896 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g16540.1

Sequence Viewer

Length: 1047 bp
ATGCATTTTTCCAACATATATAAAACTGGAAAAGCTCTCATGGCTTCCATATTGCTACTACTTGTTGTCTTGATGCCAGCCCTGCAAATAACAGGTGCACAATCTGTTGGTGTTTGTTATGGACGAAACGGCAACAATTTACCAGCTGAAGGAGAAGTGGTCGACTTGTACAAAAGCAATGGCATCGGGCGCATGATGATCTATGAACCAAATGAAGCAACCTTGCAAGCCCTAAGAGGTTCCAACATAGAACTCACTGTCACCATCCTCAACAACGAGCTTCAAGCCCTCAATGATGCTGCAGCTGCAACTGCCTGGGTCCAGAAGAACGTACAACCCTATTCAGCTGATGTTAAATTCAAATACATCGCTGTTGGAAACGAAGTGCGCCCCGGTGCTGCAGAGGTCGGGTTTCTCCTGCCTGCCATCCAAAACATCCACAGTGCAATTGTAGCAGCCAATCTGCAAGGCCAAATCAAGGTCTCTACAGCAATTGACACAAGTCTTGTGACCAATGCCTACCCTCCCTCCGATGGAGTATACACTGACCCTGCAAACCAATTCATAAAACCAGTCATCGACTTCCTCGTCAGCAATGGGGCCCCACTCCTTGTGAATGTGTACCCTTACTTCAGCTACAATGACAACCCTGGTAGCATAGACCTTGCCTATGCCTTGTTCACTTCACAAGGGGTTGTAGTGCCAGACGGGACTCGATACCCTAGCCTCTTTGACGCTCTCCTGGACGCTCAGTACGCAGCTCTTGAAAAAGCTGGTGCACCAAATGTGGAGATAGTCGTATCGGAGAGTGGTTGGCCTTCTGAAGGTGGAAATCAAGCAACCCCTGAGAACGCAGCCACATTCTACCAGAATTTGATCAAGCATGTGACGAGTACTACTGGGACTCCAAAGAGGCCTGGTAAAGCTATAGAGACTTATCTTTTTGCAATGTTTGATGAGAACCTCAAGGCAGGTAATGCAGATGAGAAACACTTTGGAATTTTTACCCCTGACAAACAACCCAAGTACCAACTCACATTTGGATAG

Protein Analysis

349

Amino Acids

37.42

Weight (kDa)

4.97

Isoelectric Point (pI)

32.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_17 PF00332 36 - 347 3.3e-108 Glycosyl hydrolases family 17
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 587
Acc36I ACCTGC 1 cut(s) 962
AccI GTMKAC 2 cut(s) 162, 540
AcsI RAATTY 3 cut(s) 356, 871, 999
AcuI CTGAAG 3 cut(s) 168, 616, 843
AfaI GTAC 6 cut(s) 170, 333, 623, 755, 895, 1028
AfiI CCNNNNNNNGG 4 cut(s) 149, 478, 533, 824
AgsI TTSAA 3 cut(s) 284, 361, 767
AjnI CCWGG 4 cut(s) 314, 649, 741, 916
AluBI AGCT 9 cut(s) 35, 146, 280, 305, 347, 636, 761, 773, 926
AluI AGCT 9 cut(s) 35, 146, 280, 305, 347, 636, 761, 773, 926
Alw21I GWGCWC 2 cut(s) 100, 781
Alw26I GTCTC 2 cut(s) 487, 926
Alw44I GTGCAC 2 cut(s) 96, 777
AoxI GGCC 4 cut(s) 469, 600, 815, 914
ApaI GGGCCC 1 cut(s) 604
ApaLI GTGCAC 2 cut(s) 96, 777
ApeKI GCWGC 7 cut(s) 299, 302, 305, 398, 455, 758, 854
ApoI RAATTY 3 cut(s) 356, 871, 999
AspLEI GCGC 2 cut(s) 192, 390
AspS9I GGNCC 3 cut(s) 319, 600, 601
AsuC2I CCSGG 1 cut(s) 393
AsuHPI GGTGA 1 cut(s) 253
AvaII GGWCC 1 cut(s) 319
BaeGI GKGCMC 3 cut(s) 100, 604, 781
BaeI ACNNNNGTAYC 2 cut(s) 1010, 1043
BanII GRGCYC 1 cut(s) 604
BarI GAAGNNNNNNTAC 2 cut(s) 614, 646
Bbv12I GWGCWC 2 cut(s) 100, 781
BbvI GCAGC 7 cut(s) 286, 292, 314, 385, 467, 770, 866
BccI CCATC 3 cut(s) 272, 434, 527
BceAI ACGGC 1 cut(s) 145
BcgI CGANNNNNNTGC 2 cut(s) 166, 200
BciT130I CCWGG 4 cut(s) 316, 651, 743, 918
BclI TGATCA 1 cut(s) 876
BcnI CCSGG 1 cut(s) 393
BcoDI GTCTC 2 cut(s) 487, 926
BfaI CTAG 1 cut(s) 723
BfmI CTRYAG 4 cut(s) 300, 399, 486, 927
BfuAI ACCTGC 1 cut(s) 962
BisI GCNGC 7 cut(s) 300, 303, 306, 399, 456, 759, 855
BlsI GCNGC 7 cut(s) 301, 304, 307, 400, 457, 760, 856
BmcAI AGTACT 1 cut(s) 895
Bme1390I CCNGG 5 cut(s) 316, 393, 651, 743, 918
Bme18I GGWCC 1 cut(s) 319
BmgT120I GGNCC 3 cut(s) 319, 600, 601
BmiI GGNNCC 5 cut(s) 241, 320, 601, 602, 603
BmrFI CCNGG 5 cut(s) 316, 393, 651, 743, 918
BmrI ACTGGG 1 cut(s) 909
BmsI GCATC 3 cut(s) 63, 192, 286
BmuI ACTGGG 1 cut(s) 909
BoxI GACNNNNGTC 1 cut(s) 501
BpuEI CTTGAG 1 cut(s) 950
BpuMI CCSGG 1 cut(s) 393
BsaI GGTCTC 1 cut(s) 487
BsaJI CCNNGG 3 cut(s) 315, 391, 649
BsaXI ACNNNNNCTCC 6 cut(s) 144, 174, 512, 542, 889, 919
Bsc4I CCNNNNNNNGG 4 cut(s) 149, 478, 533, 824
Bse1I ACTGG 3 cut(s) 31, 572, 904
Bse3DI GCAATG 3 cut(s) 184, 601, 954
BseBI CCWGG 4 cut(s) 316, 651, 743, 918
BseDI CCNNGG 3 cut(s) 315, 391, 649
BseGI GGATG 3 cut(s) 264, 426, 435
BseLI CCNNNNNNNGG 4 cut(s) 149, 478, 533, 824
BseMI GCAATG 3 cut(s) 184, 601, 954
BseMII CTCAG 2 cut(s) 764, 837
BseNI ACTGG 3 cut(s) 31, 572, 904
BseSI GKGCMC 3 cut(s) 100, 604, 781
BseXI GCAGC 7 cut(s) 286, 292, 314, 385, 467, 770, 866
BshFI GGCC 4 cut(s) 471, 602, 817, 916
BsiHKAI GWGCWC 2 cut(s) 100, 781
BsiSI CCGG 1 cut(s) 393
BslFI GGGAC 2 cut(s) 724, 916
BslI CCNNNNNNNGG 4 cut(s) 149, 478, 533, 824
BsmAI GTCTC 2 cut(s) 487, 926
BsmFI GGGAC 2 cut(s) 724, 916
BsnI GGCC 4 cut(s) 471, 602, 817, 916
Bso31I GGTCTC 1 cut(s) 487
Bsp120I GGGCCC 1 cut(s) 600
Bsp1286I GDGCHC 3 cut(s) 100, 604, 781
Bsp1407I TGTACA 1 cut(s) 168
Bsp143I GATC 2 cut(s) 198, 876
BspANI GGCC 4 cut(s) 471, 602, 817, 916
BspCNI CTCAG 2 cut(s) 763, 838
BspLI GGNNCC 5 cut(s) 241, 320, 601, 602, 603
BspMAI CTGCAG 2 cut(s) 304, 403
BspMI ACCTGC 1 cut(s) 962
BspTNI GGTCTC 1 cut(s) 487
BsrDI GCAATG 3 cut(s) 184, 601, 954
BsrGI TGTACA 1 cut(s) 168
BsrI ACTGG 3 cut(s) 31, 572, 904
BssECI CCNNGG 3 cut(s) 315, 391, 649
BssMI GATC 2 cut(s) 198, 876
BssNAI GTATAC 1 cut(s) 541
Bst1107I GTATAC 1 cut(s) 541
Bst2UI CCWGG 4 cut(s) 316, 651, 743, 918
Bst4CI ACNGT 2 cut(s) 259, 443
BstAPI GCANNNNNTGC 1 cut(s) 977
BstAUI TGTACA 1 cut(s) 168
BstC8I GCNNGC 3 cut(s) 78, 228, 423
BstDEI CTNAG 3 cut(s) 233, 750, 846
BstENI CCTNNNNNAGG 1 cut(s) 822
BstF5I GGATG 3 cut(s) 264, 426, 435
BstHHI GCGC 2 cut(s) 192, 390
BstKTI GATC 2 cut(s) 201, 879
BstMAI GTCTC 2 cut(s) 487, 926
BstMBI GATC 2 cut(s) 198, 876
BstMWI GCNNNNNNNGC 8 cut(s) 41, 82, 189, 305, 311, 452, 755, 977
BstNI CCWGG 4 cut(s) 316, 651, 743, 918
BstNSI RCATGY 1 cut(s) 887
BstPAI GACNNNNGTC 1 cut(s) 501
BstSCI CCNGG 5 cut(s) 314, 391, 649, 741, 916
BstSFI CTRYAG 4 cut(s) 300, 399, 486, 927
BstSLI GKGCMC 3 cut(s) 100, 604, 781
BstV1I GCAGC 7 cut(s) 286, 292, 314, 385, 467, 770, 866
BstZ17I GTATAC 1 cut(s) 541
BsuRI GGCC 4 cut(s) 471, 602, 817, 916
BtgZI GCGATG 1 cut(s) 352
BtsCI GGATG 3 cut(s) 264, 426, 435
BtsIMutI CAGTG 3 cut(s) 255, 448, 543
BveI ACCTGC 1 cut(s) 962
Cac8I GCNNGC 3 cut(s) 78, 228, 423
CfoI GCGC 2 cut(s) 192, 390
Cfr13I GGNCC 3 cut(s) 319, 600, 601
CseI GACGC 2 cut(s) 743, 755
Csp6I GTAC 6 cut(s) 169, 332, 622, 754, 894, 1027
CviAII CATG 3 cut(s) 40, 193, 884
CviQI GTAC 6 cut(s) 169, 332, 622, 754, 894, 1027
DdeI CTNAG 3 cut(s) 233, 750, 846
DpnI GATC 2 cut(s) 200, 878
DpnII GATC 2 cut(s) 198, 876
DrdI GACNNNNNNGTC 1 cut(s) 587
DseDI GACNNNNNNGTC 1 cut(s) 587
Eco147I AGGCCT 1 cut(s) 916
Eco24I GRGCYC 1 cut(s) 604
Eco31I GGTCTC 1 cut(s) 487
Eco47I GGWCC 1 cut(s) 319
Eco57I CTGAAG 3 cut(s) 168, 616, 843
EcoNI CCTNNNNNAGG 1 cut(s) 822
EcoO109I RGGNCCY 2 cut(s) 600, 601
EcoRII CCWGG 4 cut(s) 314, 649, 741, 916
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 604
FaeI CATG 3 cut(s) 43, 196, 887
FaqI GGGAC 2 cut(s) 724, 916
FatI CATG 3 cut(s) 39, 192, 883
FbaI TGATCA 1 cut(s) 876
FblI GTMKAC 2 cut(s) 162, 540
Fnu4HI GCNGC 7 cut(s) 300, 303, 306, 399, 456, 759, 855
FokI GGATG 3 cut(s) 251, 413, 422
FriOI GRGCYC 1 cut(s) 604
Fsp4HI GCNGC 7 cut(s) 300, 303, 306, 399, 456, 759, 855
FspBI CTAG 1 cut(s) 723
GlaI GCGC 2 cut(s) 191, 389
GluI GCNGC 7 cut(s) 300, 303, 306, 399, 456, 759, 855
HaeIII GGCC 4 cut(s) 471, 602, 817, 916
HapII CCGG 1 cut(s) 393
HgaI GACGC 2 cut(s) 743, 755
HhaI GCGC 2 cut(s) 192, 390
Hin1II CATG 3 cut(s) 43, 196, 887
Hin6I GCGC 2 cut(s) 190, 388
HinP1I GCGC 2 cut(s) 190, 388
HincII GTYRAC 1 cut(s) 163
HindII GTYRAC 1 cut(s) 163
HinfI GANTC 2 cut(s) 712, 904
HpaII CCGG 1 cut(s) 393
HphI GGTGA 1 cut(s) 253
Hpy166II GTNNAC 6 cut(s) 98, 163, 541, 622, 681, 779
Hpy188I TCNGA 3 cut(s) 532, 805, 823
Hpy188III TCNNGA 3 cut(s) 70, 322, 764
Hpy8I GTNNAC 6 cut(s) 98, 163, 541, 622, 681, 779
HpyAV CCTTC 3 cut(s) 143, 818, 828
HpyCH4III ACNGT 2 cut(s) 259, 443
HpyCH4IV ACGT 1 cut(s) 330
HpyF10VI GCNNNNNNNGC 8 cut(s) 41, 82, 189, 305, 311, 452, 755, 977
HpyF3I CTNAG 3 cut(s) 233, 750, 846
HpySE526I ACGT 1 cut(s) 330
Hsp92II CATG 3 cut(s) 43, 196, 887
HspAI GCGC 2 cut(s) 190, 388
Ksp22I TGATCA 1 cut(s) 876
Kzo9I GATC 2 cut(s) 198, 876
Lsp1109I GCAGC 7 cut(s) 286, 292, 314, 385, 467, 770, 866
LweI GCATC 3 cut(s) 63, 192, 286
MaeI CTAG 1 cut(s) 723
MaeII ACGT 1 cut(s) 330
MaeIII GTNAC 3 cut(s) 259, 508, 886
MalI GATC 2 cut(s) 200, 878
MboI GATC 2 cut(s) 198, 876
MboII GAAGA 1 cut(s) 337
MfeI CAATTG 2 cut(s) 447, 492
MhlI GDGCHC 3 cut(s) 100, 604, 781
MluCI AATT 7 cut(s) 136, 356, 447, 492, 560, 871, 999
MlyI GAGTC 2 cut(s) 706, 898
MmeI TCCRAC 3 cut(s) 36, 267, 355
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 1 cut(s) 354
MspA1I CMGCKG 3 cut(s) 146, 305, 347
MspI CCGG 1 cut(s) 393
MspR9I CCNGG 5 cut(s) 316, 393, 651, 743, 918
MunI CAATTG 2 cut(s) 447, 492
MvaI CCWGG 4 cut(s) 316, 651, 743, 918
MwoI GCNNNNNNNGC 8 cut(s) 41, 82, 189, 305, 311, 452, 755, 977
NciI CCSGG 1 cut(s) 393
NdeII GATC 2 cut(s) 198, 876
NlaIII CATG 3 cut(s) 43, 196, 887
NlaIV GGNNCC 5 cut(s) 241, 320, 601, 602, 603
NmuCI GTSAC 3 cut(s) 259, 508, 886
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 887
PceI AGGCCT 1 cut(s) 916
PcsI WCGNNNNNNNCGW 1 cut(s) 585
PfoI TCCNGGA 1 cut(s) 741
PkrI GCNGC 7 cut(s) 301, 304, 307, 400, 457, 760, 856
PleI GAGTC 2 cut(s) 706, 898
PpsI GAGTC 2 cut(s) 706, 898
PshAI GACNNNNGTC 1 cut(s) 501
Psp6I CCWGG 4 cut(s) 314, 649, 741, 916
PspGI CCWGG 4 cut(s) 314, 649, 741, 916
PspN4I GGNNCC 5 cut(s) 241, 320, 601, 602, 603
PspOMI GGGCCC 1 cut(s) 600
PspPI GGNCC 3 cut(s) 319, 600, 601
PstI CTGCAG 2 cut(s) 304, 403
PvuII CAGCTG 3 cut(s) 146, 305, 347
RsaI GTAC 6 cut(s) 170, 333, 623, 755, 895, 1028
RsaNI GTAC 6 cut(s) 169, 332, 622, 754, 894, 1027
SalI GTCGAC 1 cut(s) 161
SaqAI TTAA 1 cut(s) 354
SatI GCNGC 7 cut(s) 300, 303, 306, 399, 456, 759, 855
Sau3AI GATC 2 cut(s) 198, 876
Sau96I GGNCC 3 cut(s) 319, 600, 601
ScaI AGTACT 1 cut(s) 895
SchI GAGTC 2 cut(s) 706, 898
ScrFI CCNGG 5 cut(s) 316, 393, 651, 743, 918
SduI GDGCHC 3 cut(s) 100, 604, 781
SfaNI GCATC 3 cut(s) 63, 192, 286
SfcI CTRYAG 4 cut(s) 300, 399, 486, 927
SinI GGWCC 1 cut(s) 319
SmlI CTYRAG 1 cut(s) 965
SmoI CTYRAG 1 cut(s) 965
Sse9I AATT 7 cut(s) 136, 356, 447, 492, 560, 871, 999
SseBI AGGCCT 1 cut(s) 916
SspMI CTAG 1 cut(s) 723
StuI AGGCCT 1 cut(s) 916
StyD4I CCNGG 5 cut(s) 314, 391, 649, 741, 916
TaaI ACNGT 2 cut(s) 259, 443
TaiI ACGT 1 cut(s) 333
TaqI TCGA 3 cut(s) 162, 579, 715
TasI AATT 7 cut(s) 136, 356, 447, 492, 560, 871, 999
TatI WGTACW 2 cut(s) 168, 893
Tru1I TTAA 1 cut(s) 354
Tru9I TTAA 1 cut(s) 354
TscAI CASTG 3 cut(s) 262, 448, 550
TseFI GTSAC 3 cut(s) 259, 508, 886
TseI GCWGC 7 cut(s) 299, 302, 305, 398, 455, 758, 854
Tsp45I GTSAC 3 cut(s) 259, 508, 886
TspDTI ATGAA 3 cut(s) 219, 228, 553
TspRI CASTG 3 cut(s) 262, 448, 550
VneI GTGCAC 2 cut(s) 96, 777
VpaK11BI GGWCC 1 cut(s) 319
XagI CCTNNNNNAGG 1 cut(s) 822
XapI RAATTY 3 cut(s) 356, 871, 999
XceI RCATGY 1 cut(s) 887
XcmI CCANNNNNNNNNTGG 1 cut(s) 1037
XmiI GTMKAC 2 cut(s) 162, 540
XspI CTAG 1 cut(s) 723
ZrmI AGTACT 1 cut(s) 895
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.