MD03G1171500.v1.1

Belongs to the glycosyl hydrolase 17 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0008128
Physical Location & Seq
Forward (+)
23311267 .. 23313657
2391 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1171500.v1.1.491

Sequence Viewer

Length: 1047 bp
ATGCATTTTTCCGACATATATAAAATTGGGAAAGCTCTCATGGCTTCCATATTGCTACTACTTGTACTCTTGATGCCTGCCCTGGAAATAACAGGTGCACAATCTGTTGGTGTTTGCTATGGACGAAACGGCGACAATTTACCAGCTGAAGGAGAAGTGGTCGACTTGTACAAAAGCAATGGCATCGGACGGATGCGGATGTATGAACCAAATGAAGCAACCTTCCAAGCCCTAAGAGGTTCCAACATAGAACTCACTCTAACCATCCTCAACAGCGAGCTTTCAGCCCTCAATGATGCTGCTGCTGCAACTGCCTGGGTCCAGAAAAACGTACAACCCTATTCAGCCGATGTCCGATTCAAATACATCGCTGTTGGAAACGAAGTACACCCTGGTACTGCAGAGGCCGGGTTTCTCCTGCCTGCCATCCAAAACATCCACAGTGCAATTGTAGCGGCCAATCTCCAAGGCCAAATCAAAGTCTCAACAGCAATTGACACAACTCTTGTGACCAACGCCTACCCTCCCTCAGATGGAATATATACTGACGCTGCAAATCCATTCATAACACCTATCATCAATTTCCTCGTCAGCAATGGGGCCCCACTCCTCGTCAATGTGTACCCTTACTTCAGCTACAATGGTGACCCTAATAACATAGACCTTGGCTATGCCTTGTTCACTTCACAAGGGGTCGTGGTACCGGACGGAACTCGATACCCTAGCCTCTTTGATGCTCTTTTGGATGCTCAGTACTCGGCTCTTGAAAAAGCTGGCGCACCAAATATAGAGATAGTGGTATCGGAGAGCGGTTGGCCTTCTGAAGGTGGTAATCAAGCAACCGCTCAGAACGCAGCCACATTCTACCAGAACTTGATCAGCCATGTGACGAGTACTACTGGGACTCCAAAGAGGCCTGGGAGAGCTATAGAGACTTATCTTTTTGCATTGTTTGATGAGAATCTCAAGGGCCCTGAACCAGTTGAGAAACACTTCGGAGTTTTCTCCCCTAACAAACAACACAAGTACCAACTCACCTTCGGATAA

Protein Analysis

349

Amino Acids

37.48

Weight (kDa)

4.96

Isoelectric Point (pI)

37.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_17 PF00332 36 - 347 9.4e-109 Glycosyl hydrolases family 17
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 700
AccB1I GGYRCC 1 cut(s) 700
AccBSI CCGCTC 2 cut(s) 810, 845
AccI GTMKAC 1 cut(s) 162
AciI CCGC 4 cut(s) 196, 455, 810, 843
AcoI YGGCCR 1 cut(s) 456
AcuI CTGAAG 3 cut(s) 168, 616, 843
AfiI CCNNNNNNNGG 3 cut(s) 149, 533, 824
AgsI TTSAA 2 cut(s) 361, 767
AjnI CCWGG 4 cut(s) 81, 314, 391, 916
AluBI AGCT 6 cut(s) 35, 146, 280, 636, 773, 926
AluI AGCT 6 cut(s) 35, 146, 280, 636, 773, 926
Alw21I GWGCWC 1 cut(s) 100
Alw26I GTCTC 2 cut(s) 487, 926
Alw44I GTGCAC 1 cut(s) 96
AoxI GGCC 7 cut(s) 405, 456, 469, 600, 815, 914, 970
ApaI GGGCCC 2 cut(s) 604, 974
ApaLI GTGCAC 1 cut(s) 96
ApeKI GCWGC 5 cut(s) 299, 302, 305, 551, 854
Asp700I GAANNNNTTC 1 cut(s) 992
Asp718I GGTACC 1 cut(s) 700
AspLEI GCGC 1 cut(s) 779
AspS9I GGNCC 5 cut(s) 319, 600, 601, 970, 971
AsuC2I CCSGG 1 cut(s) 409
AsuHPI GGTGA 2 cut(s) 656, 1027
AvaII GGWCC 1 cut(s) 319
BaeGI GKGCMC 3 cut(s) 100, 604, 974
BaeI ACNNNNGTAYC 2 cut(s) 1010, 1043
BanI GGYRCC 1 cut(s) 700
BanII GRGCYC 2 cut(s) 604, 974
BarI GAAGNNNNNNTAC 2 cut(s) 614, 646
Bbv12I GWGCWC 1 cut(s) 100
BbvI GCAGC 5 cut(s) 286, 289, 292, 538, 866
BccI CCATC 3 cut(s) 272, 434, 527
BceAI ACGGC 1 cut(s) 145
BcgI CGANNNNNNTGC 2 cut(s) 166, 200
BciT130I CCWGG 4 cut(s) 83, 316, 393, 918
BclI TGATCA 1 cut(s) 876
BcnI CCSGG 1 cut(s) 409
BcoDI GTCTC 2 cut(s) 487, 926
BfaI CTAG 1 cut(s) 723
BfmI CTRYAG 2 cut(s) 399, 927
BisI GCNGC 6 cut(s) 300, 303, 306, 456, 552, 855
BlsI GCNGC 6 cut(s) 301, 304, 307, 457, 553, 856
BmcAI AGTACT 2 cut(s) 755, 895
Bme1390I CCNGG 5 cut(s) 83, 316, 393, 409, 918
Bme18I GGWCC 1 cut(s) 319
BmgT120I GGNCC 5 cut(s) 319, 600, 601, 970, 971
BmiI GGNNCC 7 cut(s) 241, 320, 601, 602, 603, 702, 972
BmrFI CCNGG 5 cut(s) 83, 316, 393, 409, 918
BmrI ACTGGG 1 cut(s) 909
BmsI GCATC 6 cut(s) 63, 183, 192, 286, 724, 736
BmuI ACTGGG 1 cut(s) 909
BpuEI CTTGAG 1 cut(s) 950
BpuMI CCSGG 1 cut(s) 409
BsaBI GATNNNNATC 1 cut(s) 960
BsaJI CCNNGG 6 cut(s) 81, 315, 391, 466, 664, 917
BsaWI WCCGGW 1 cut(s) 703
BsaXI ACNNNNNCTCC 4 cut(s) 144, 174, 889, 919
Bsc4I CCNNNNNNNGG 3 cut(s) 149, 533, 824
Bse1I ACTGG 2 cut(s) 904, 980
Bse3DI GCAATG 2 cut(s) 184, 601
Bse8I GATNNNNATC 1 cut(s) 960
BseBI CCWGG 4 cut(s) 83, 316, 393, 918
BseDI CCNNGG 6 cut(s) 81, 315, 391, 466, 664, 917
BseGI GGATG 6 cut(s) 198, 204, 264, 426, 435, 751
BseJI GATNNNNATC 1 cut(s) 960
BseLI CCNNNNNNNGG 3 cut(s) 149, 533, 824
BseMI GCAATG 2 cut(s) 184, 601
BseMII CTCAG 3 cut(s) 543, 764, 860
BseNI ACTGG 2 cut(s) 904, 980
BseRI GAGGAG 1 cut(s) 599
BseSI GKGCMC 3 cut(s) 100, 604, 974
BseXI GCAGC 5 cut(s) 286, 289, 292, 538, 866
BshFI GGCC 7 cut(s) 407, 458, 471, 602, 817, 916, 972
BshNI GGYRCC 1 cut(s) 700
BsiHKAI GWGCWC 1 cut(s) 100
BsiSI CCGG 2 cut(s) 408, 704
BslFI GGGAC 1 cut(s) 916
BslI CCNNNNNNNGG 3 cut(s) 149, 533, 824
BsmAI GTCTC 2 cut(s) 487, 926
BsmFI GGGAC 1 cut(s) 916
BsnI GGCC 7 cut(s) 407, 458, 471, 602, 817, 916, 972
Bsp120I GGGCCC 2 cut(s) 600, 970
Bsp1286I GDGCHC 3 cut(s) 100, 604, 974
Bsp1407I TGTACA 1 cut(s) 168
Bsp143I GATC 1 cut(s) 876
BspACI CCGC 4 cut(s) 196, 455, 810, 843
BspANI GGCC 7 cut(s) 407, 458, 471, 602, 817, 916, 972
BspCNI CTCAG 3 cut(s) 542, 763, 859
BspLI GGNNCC 7 cut(s) 241, 320, 601, 602, 603, 702, 972
BspMAI CTGCAG 1 cut(s) 403
BspT107I GGYRCC 1 cut(s) 700
BsrBI CCGCTC 2 cut(s) 810, 845
BsrDI GCAATG 2 cut(s) 184, 601
BsrGI TGTACA 1 cut(s) 168
BsrI ACTGG 2 cut(s) 904, 980
BssECI CCNNGG 6 cut(s) 81, 315, 391, 466, 664, 917
BssMI GATC 1 cut(s) 876
BssT1I CCWWGG 2 cut(s) 466, 664
Bst2UI CCWGG 4 cut(s) 83, 316, 393, 918
Bst4CI ACNGT 1 cut(s) 443
BstAUI TGTACA 1 cut(s) 168
BstC8I GCNNGC 4 cut(s) 78, 278, 423, 775
BstDEI CTNAG 4 cut(s) 233, 529, 750, 846
BstEII GGTNACC 1 cut(s) 644
BstENI CCTNNNNNAGG 1 cut(s) 822
BstF5I GGATG 6 cut(s) 198, 204, 264, 426, 435, 751
BstHHI GCGC 1 cut(s) 779
BstKTI GATC 1 cut(s) 879
BstMAI GTCTC 2 cut(s) 487, 926
BstMBI GATC 1 cut(s) 876
BstMWI GCNNNNNNNGC 5 cut(s) 41, 305, 311, 452, 851
BstNI CCWGG 4 cut(s) 83, 316, 393, 918
BstPI GGTNACC 1 cut(s) 644
BstSCI CCNGG 5 cut(s) 81, 314, 391, 407, 916
BstSFI CTRYAG 2 cut(s) 399, 927
BstSLI GKGCMC 3 cut(s) 100, 604, 974
BstV1I GCAGC 5 cut(s) 286, 289, 292, 538, 866
BsuRI GGCC 7 cut(s) 407, 458, 471, 602, 817, 916, 972
BtgZI GCGATG 1 cut(s) 352
BtsCI GGATG 6 cut(s) 198, 204, 264, 426, 435, 751
BtsIMutI CAGTG 1 cut(s) 448
Cac8I GCNNGC 4 cut(s) 78, 278, 423, 775
CfoI GCGC 1 cut(s) 779
Cfr13I GGNCC 5 cut(s) 319, 600, 601, 970, 971
CseI GACGC 1 cut(s) 557
CviAII CATG 2 cut(s) 40, 884
DdeI CTNAG 4 cut(s) 233, 529, 750, 846
DpnI GATC 1 cut(s) 878
DpnII GATC 1 cut(s) 876
EaeI YGGCCR 1 cut(s) 456
Eco130I CCWWGG 2 cut(s) 466, 664
Eco147I AGGCCT 1 cut(s) 916
Eco24I GRGCYC 2 cut(s) 604, 974
Eco47I GGWCC 1 cut(s) 319
Eco57I CTGAAG 3 cut(s) 168, 616, 843
Eco91I GGTNACC 1 cut(s) 644
EcoNI CCTNNNNNAGG 1 cut(s) 822
EcoO109I RGGNCCY 4 cut(s) 600, 601, 970, 971
EcoO65I GGTNACC 1 cut(s) 644
EcoRII CCWGG 4 cut(s) 81, 314, 391, 916
EcoT14I CCWWGG 2 cut(s) 466, 664
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 2 cut(s) 604, 974
ErhI CCWWGG 2 cut(s) 466, 664
FaeI CATG 2 cut(s) 43, 887
FaqI GGGAC 1 cut(s) 916
FatI CATG 2 cut(s) 39, 883
FbaI TGATCA 1 cut(s) 876
FblI GTMKAC 1 cut(s) 162
Fnu4HI GCNGC 6 cut(s) 300, 303, 306, 456, 552, 855
FokI GGATG 6 cut(s) 205, 211, 251, 413, 422, 758
FriOI GRGCYC 2 cut(s) 604, 974
Fsp4HI GCNGC 6 cut(s) 300, 303, 306, 456, 552, 855
FspBI CTAG 1 cut(s) 723
GlaI GCGC 1 cut(s) 778
GluI GCNGC 6 cut(s) 300, 303, 306, 456, 552, 855
HaeIII GGCC 7 cut(s) 407, 458, 471, 602, 817, 916, 972
HapII CCGG 2 cut(s) 408, 704
HgaI GACGC 1 cut(s) 557
HhaI GCGC 1 cut(s) 779
Hin1II CATG 2 cut(s) 43, 887
Hin6I GCGC 1 cut(s) 777
HinP1I GCGC 1 cut(s) 777
HincII GTYRAC 1 cut(s) 163
HindII GTYRAC 1 cut(s) 163
HinfI GANTC 3 cut(s) 357, 904, 961
HpaII CCGG 2 cut(s) 408, 704
HphI GGTGA 2 cut(s) 656, 1027
Hpy166II GTNNAC 5 cut(s) 98, 163, 388, 622, 681
Hpy188I TCNGA 9 cut(s) 13, 188, 356, 532, 805, 823, 849, 998, 1043
Hpy188III TCNNGA 3 cut(s) 70, 322, 764
Hpy8I GTNNAC 5 cut(s) 98, 163, 388, 622, 681
HpyAV CCTTC 4 cut(s) 143, 232, 818, 828
HpyCH4III ACNGT 1 cut(s) 443
HpyCH4IV ACGT 1 cut(s) 330
HpyCH4V TGCA 7 cut(s) 4, 98, 308, 401, 446, 554, 947
HpyF10VI GCNNNNNNNGC 5 cut(s) 41, 305, 311, 452, 851
HpyF3I CTNAG 4 cut(s) 233, 529, 750, 846
HpySE526I ACGT 1 cut(s) 330
Hsp92II CATG 2 cut(s) 43, 887
HspAI GCGC 1 cut(s) 777
KpnI GGTACC 1 cut(s) 704
Ksp22I TGATCA 1 cut(s) 876
Kzo9I GATC 1 cut(s) 876
Lsp1109I GCAGC 5 cut(s) 286, 289, 292, 538, 866
LweI GCATC 6 cut(s) 63, 183, 192, 286, 724, 736
MaeI CTAG 1 cut(s) 723
MaeII ACGT 1 cut(s) 330
MaeIII GTNAC 3 cut(s) 508, 644, 886
MalI GATC 1 cut(s) 878
MbiI CCGCTC 2 cut(s) 810, 845
MboI GATC 1 cut(s) 876
MfeI CAATTG 2 cut(s) 447, 492
MhlI GDGCHC 3 cut(s) 100, 604, 974
MluCI AATT 5 cut(s) 24, 136, 447, 492, 580
MlyI GAGTC 1 cut(s) 898
MmeI TCCRAC 3 cut(s) 36, 267, 355
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 1 cut(s) 992
MspA1I CMGCKG 1 cut(s) 146
MspI CCGG 2 cut(s) 408, 704
MspR9I CCNGG 5 cut(s) 83, 316, 393, 409, 918
MunI CAATTG 2 cut(s) 447, 492
MvaI CCWGG 4 cut(s) 83, 316, 393, 918
MwoI GCNNNNNNNGC 5 cut(s) 41, 305, 311, 452, 851
NciI CCSGG 1 cut(s) 409
NdeII GATC 1 cut(s) 876
NlaIII CATG 2 cut(s) 43, 887
NlaIV GGNNCC 7 cut(s) 241, 320, 601, 602, 603, 702, 972
NmeAIII GCCGAG 1 cut(s) 737
NmuCI GTSAC 3 cut(s) 508, 644, 886
NsiI ATGCAT 1 cut(s) 6
PceI AGGCCT 1 cut(s) 916
PdmI GAANNNNTTC 1 cut(s) 992
PfeI GAWTC 2 cut(s) 357, 961
PkrI GCNGC 6 cut(s) 301, 304, 307, 457, 553, 856
PleI GAGTC 1 cut(s) 898
PpsI GAGTC 1 cut(s) 898
Psp6I CCWGG 4 cut(s) 81, 314, 391, 916
PspEI GGTNACC 1 cut(s) 644
PspGI CCWGG 4 cut(s) 81, 314, 391, 916
PspN4I GGNNCC 7 cut(s) 241, 320, 601, 602, 603, 702, 972
PspOMI GGGCCC 2 cut(s) 600, 970
PspPI GGNCC 5 cut(s) 319, 600, 601, 970, 971
PstI CTGCAG 1 cut(s) 403
PvuII CAGCTG 1 cut(s) 146
SalI GTCGAC 1 cut(s) 161
SatI GCNGC 6 cut(s) 300, 303, 306, 456, 552, 855
Sau3AI GATC 1 cut(s) 876
Sau96I GGNCC 5 cut(s) 319, 600, 601, 970, 971
ScaI AGTACT 2 cut(s) 755, 895
SchI GAGTC 1 cut(s) 898
ScrFI CCNGG 5 cut(s) 83, 316, 393, 409, 918
SduI GDGCHC 3 cut(s) 100, 604, 974
SfaNI GCATC 6 cut(s) 63, 183, 192, 286, 724, 736
SfcI CTRYAG 2 cut(s) 399, 927
SinI GGWCC 1 cut(s) 319
SmlI CTYRAG 1 cut(s) 965
SmoI CTYRAG 1 cut(s) 965
Sse9I AATT 5 cut(s) 24, 136, 447, 492, 580
SseBI AGGCCT 1 cut(s) 916
SsiI CCGC 4 cut(s) 196, 455, 810, 843
SspMI CTAG 1 cut(s) 723
StuI AGGCCT 1 cut(s) 916
StyD4I CCNGG 5 cut(s) 81, 314, 391, 407, 916
StyI CCWWGG 2 cut(s) 466, 664
TaaI ACNGT 1 cut(s) 443
TaiI ACGT 1 cut(s) 333
TaqI TCGA 2 cut(s) 162, 715
TasI AATT 5 cut(s) 24, 136, 447, 492, 580
TatI WGTACW 5 cut(s) 64, 168, 385, 753, 893
TauI GCSGC 1 cut(s) 458
TfiI GAWTC 2 cut(s) 357, 961
TscAI CASTG 1 cut(s) 448
TseFI GTSAC 3 cut(s) 508, 644, 886
TseI GCWGC 5 cut(s) 299, 302, 305, 551, 854
Tsp45I GTSAC 3 cut(s) 508, 644, 886
TspDTI ATGAA 3 cut(s) 219, 228, 553
TspGWI ACGGA 2 cut(s) 205, 723
TspRI CASTG 1 cut(s) 448
VneI GTGCAC 1 cut(s) 96
VpaK11BI GGWCC 1 cut(s) 319
XagI CCTNNNNNAGG 1 cut(s) 822
XmiI GTMKAC 1 cut(s) 162
XmnI GAANNNNTTC 1 cut(s) 992
XspI CTAG 1 cut(s) 723
ZrmI AGTACT 2 cut(s) 755, 895
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.