Rw5G032090

Belongs to the glycosyl hydrolase 17 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0008128
Physical Location & Seq
Forward (+)
53913101 .. 53915073
1973 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G032090.1

Sequence Viewer

Length: 1032 bp
ATGGATTTTTCCTACACAACACATAAAAATACCTTCATGGCTCCCATACTGCTGCTCCTTGTACTGTTGATGCCTGCTCTACAAATGACAGGTGCACAATCTGTGGGTGTCTGCTATGGACGAAATGGCGACAATTTACCTACTGATACAGAAGTTGTTGATCTGTACAAAAGCAATGGAATTGGAAGGATGAGGATATACGAGCCAGACCAAGCAACCTTGGAGGCCCTTAAAGGTTCTAACATAGAACTCATAGTCACCATCCTCAACAACCACCTTCAAGAGCTCACTGATGCGGCAGCAGCTACAAATTGGGTCCAGCAAAATGTGCAACCTTACGCGGCTGATGTCAAATTCAAGTACATTGCTGTTGGCAATGAAGTACACCCTGATGCTGCAGAGGCAAAATATCTTCTTCCTGCCATACAGAACATTCAGAACGCGGTTACAGCCGCCAATCTACAAGTCAAAGTGTCAACAGCAATAGATACAACTCTTACCCAAAACTTCCCTCCTTCAAATGGGGCATTCAGCGATGCTGCGAATTCATTTATTACCCCAATTATCACCTTCTTAGGCAACAATGGATCACCACTTCTTGCCAATATATACCCCTACTTCGCCTATGTCGGTAACCCTGCTGACATAAAACTAGAATATGCCTTGTTCACTTCACCAGGGGTAGTGGTACAAGATGGTAGTAATGGGTACCAGAACCTCTTTGATGCTTTGTTGGATACTCTTTACTCTGCTCTTGAAAAAGCAGGGGCACCCAATATGGCAATAGTTGTATCAGAGAGCGGTTGGCCATCTGCAGGTAGTGATGCTGCAACTACTGGTAATGCAGGAACATACTACAGCAATTTGATCAAGCATGTGAAGACCGGGACTCCGAAGAGGCCTAATGGAGGTATCGAAACTTATCTGTTTGCCATGTTTGATGAAAACCTCAAGACTGGTGACGAAGTTGAGAAACATTTTGGTCTCTTCTTCCCTAACAAACAGCCGAAGTACCAACTCACCTTTGGCTAG

Protein Analysis

343

Amino Acids

37.11

Weight (kDa)

4.85

Isoelectric Point (pI)

29.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_17 PF00332 35 - 342 4.3e-108 Glycosyl hydrolases family 17
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 806
Acc65I GGTACC 1 cut(s) 708
AccB1I GGYRCC 2 cut(s) 708, 769
AccBSI CCGCTC 1 cut(s) 801
AccII CGCG 2 cut(s) 341, 443
AciI CCGC 5 cut(s) 296, 341, 443, 453, 801
AclWI GGATC 1 cut(s) 595
AcoI YGGCCR 1 cut(s) 806
AcsI RAATTY 2 cut(s) 353, 544
AfaI GTAC 7 cut(s) 63, 167, 362, 384, 690, 710, 1013
AfiI CCNNNNNNNGG 3 cut(s) 521, 815, 908
AgsI TTSAA 4 cut(s) 281, 358, 519, 758
AjnI CCWGG 1 cut(s) 676
AluBI AGCT 2 cut(s) 286, 305
AluI AGCT 2 cut(s) 286, 305
Alw21I GWGCWC 2 cut(s) 97, 288
Alw26I GTCTC 1 cut(s) 989
Alw44I GTGCAC 1 cut(s) 93
AlwI GGATC 1 cut(s) 595
AoxI GGCC 3 cut(s) 225, 806, 899
ApaLI GTGCAC 1 cut(s) 93
ApeKI GCWGC 6 cut(s) 52, 299, 302, 395, 539, 827
ApoI RAATTY 2 cut(s) 353, 544
Asp718I GGTACC 1 cut(s) 708
AspS9I GGNCC 2 cut(s) 226, 316
AsuC2I CCSGG 1 cut(s) 886
AsuHPI GGTGA 6 cut(s) 250, 559, 582, 666, 971, 1012
AvaII GGWCC 1 cut(s) 316
BaeGI GKGCMC 2 cut(s) 97, 772
BaeI ACNNNNGTAYC 2 cut(s) 138, 171
BalI TGGCCA 1 cut(s) 808
BanI GGYRCC 2 cut(s) 708, 769
BanII GRGCYC 1 cut(s) 288
BbsI GAAGAC 1 cut(s) 887
Bbv12I GWGCWC 2 cut(s) 97, 288
BbvI GCAGC 6 cut(s) 39, 311, 314, 382, 526, 814
BccI CCATC 3 cut(s) 269, 689, 817
BciT130I CCWGG 1 cut(s) 678
BciVI GTATCC 1 cut(s) 730
BclI TGATCA 1 cut(s) 867
BcnI CCSGG 1 cut(s) 886
BcoDI GTCTC 1 cut(s) 989
BfaI CTAG 2 cut(s) 653, 1030
BfmI CTRYAG 3 cut(s) 396, 813, 856
BfuAI ACCTGC 1 cut(s) 806
BfuI GTATCC 1 cut(s) 730
BisI GCNGC 9 cut(s) 53, 297, 300, 303, 342, 396, 453, 540, 828
BlsI GCNGC 9 cut(s) 54, 298, 301, 304, 343, 397, 454, 541, 829
Bme1390I CCNGG 2 cut(s) 678, 886
Bme18I GGWCC 1 cut(s) 316
BmgT120I GGNCC 2 cut(s) 226, 316
BmiI GGNNCC 4 cut(s) 42, 317, 710, 771
BmrFI CCNGG 2 cut(s) 678, 886
BmsI GCATC 6 cut(s) 60, 283, 382, 526, 715, 814
BpiI GAAGAC 1 cut(s) 887
BpuEI CTTGAG 1 cut(s) 935
BpuMI CCSGG 1 cut(s) 886
BsaI GGTCTC 1 cut(s) 989
BsaJI CCNNGG 2 cut(s) 219, 677
BsaXI ACNNNNNCTCC 4 cut(s) 39, 69, 874, 904
Bsc4I CCNNNNNNNGG 3 cut(s) 521, 815, 908
Bse1I ACTGG 2 cut(s) 841, 961
Bse3DI GCAATG 3 cut(s) 181, 363, 382
BseBI CCWGG 1 cut(s) 678
BseDI CCNNGG 2 cut(s) 219, 677
BseGI GGATG 2 cut(s) 195, 261
BseLI CCNNNNNNNGG 3 cut(s) 521, 815, 908
BseMI GCAATG 3 cut(s) 181, 363, 382
BseNI ACTGG 2 cut(s) 841, 961
BseSI GKGCMC 2 cut(s) 97, 772
BseXI GCAGC 6 cut(s) 39, 311, 314, 382, 526, 814
Bsh1236I CGCG 2 cut(s) 341, 443
BshFI GGCC 3 cut(s) 227, 808, 901
BshNI GGYRCC 2 cut(s) 708, 769
BsiHKAI GWGCWC 2 cut(s) 97, 288
BsiSI CCGG 1 cut(s) 885
BslFI GGGAC 1 cut(s) 901
BslI CCNNNNNNNGG 3 cut(s) 521, 815, 908
BsmAI GTCTC 1 cut(s) 989
BsmFI GGGAC 1 cut(s) 901
BsmI GAATGC 1 cut(s) 527
BsnI GGCC 3 cut(s) 227, 808, 901
Bso31I GGTCTC 1 cut(s) 989
Bsp1286I GDGCHC 3 cut(s) 97, 288, 772
Bsp1407I TGTACA 1 cut(s) 165
Bsp143I GATC 3 cut(s) 160, 587, 867
BspACI CCGC 5 cut(s) 296, 341, 443, 453, 801
BspANI GGCC 3 cut(s) 227, 808, 901
BspFNI CGCG 2 cut(s) 341, 443
BspLI GGNNCC 4 cut(s) 42, 317, 710, 771
BspMAI CTGCAG 2 cut(s) 400, 817
BspMI ACCTGC 1 cut(s) 806
BspPI GGATC 1 cut(s) 595
BspT107I GGYRCC 2 cut(s) 708, 769
BspTNI GGTCTC 1 cut(s) 989
BsrBI CCGCTC 1 cut(s) 801
BsrDI GCAATG 3 cut(s) 181, 363, 382
BsrGI TGTACA 1 cut(s) 165
BsrI ACTGG 2 cut(s) 841, 961
BssECI CCNNGG 2 cut(s) 219, 677
BssMI GATC 3 cut(s) 160, 587, 867
BssT1I CCWWGG 1 cut(s) 219
Bst2UI CCWGG 1 cut(s) 678
Bst4CI ACNGT 1 cut(s) 66
Bst6I CTCTTC 2 cut(s) 890, 992
BstAPI GCANNNNNTGC 1 cut(s) 328
BstAUI TGTACA 1 cut(s) 165
BstC8I GCNNGC 1 cut(s) 75
BstDEI CTNAG 1 cut(s) 574
BstEII GGTNACC 1 cut(s) 632
BstENI CCTNNNNNAGG 1 cut(s) 906
BstF5I GGATG 2 cut(s) 195, 261
BstFNI CGCG 2 cut(s) 341, 443
BstKTI GATC 3 cut(s) 163, 590, 870
BstMAI GTCTC 1 cut(s) 989
BstMBI GATC 3 cut(s) 160, 587, 867
BstMWI GCNNNNNNNGC 4 cut(s) 302, 328, 401, 449
BstNI CCWGG 1 cut(s) 678
BstNSI RCATGY 1 cut(s) 878
BstPI GGTNACC 1 cut(s) 632
BstSCI CCNGG 2 cut(s) 676, 884
BstSFI CTRYAG 3 cut(s) 396, 813, 856
BstSLI GKGCMC 2 cut(s) 97, 772
BstUI CGCG 2 cut(s) 341, 443
BstV1I GCAGC 6 cut(s) 39, 311, 314, 382, 526, 814
BstV2I GAAGAC 1 cut(s) 887
BsuI GTATCC 1 cut(s) 730
BsuRI GGCC 3 cut(s) 227, 808, 901
BtgZI GCGATG 1 cut(s) 549
BtsCI GGATG 2 cut(s) 195, 261
BtsIMutI CAGTG 1 cut(s) 288
BveI ACCTGC 1 cut(s) 806
Cac8I GCNNGC 1 cut(s) 75
Cfr13I GGNCC 2 cut(s) 226, 316
Csp6I GTAC 7 cut(s) 62, 166, 361, 383, 689, 709, 1012
CviAII CATG 3 cut(s) 37, 875, 934
CviQI GTAC 7 cut(s) 62, 166, 361, 383, 689, 709, 1012
DdeI CTNAG 1 cut(s) 574
DpnI GATC 3 cut(s) 162, 589, 869
DpnII GATC 3 cut(s) 160, 587, 867
EaeI YGGCCR 1 cut(s) 806
Eam1104I CTCTTC 2 cut(s) 890, 992
EarI CTCTTC 2 cut(s) 890, 992
Ecl136II GAGCTC 1 cut(s) 286
Eco130I CCWWGG 1 cut(s) 219
Eco147I AGGCCT 1 cut(s) 901
Eco24I GRGCYC 1 cut(s) 288
Eco31I GGTCTC 1 cut(s) 989
Eco47I GGWCC 1 cut(s) 316
Eco53kI GAGCTC 1 cut(s) 286
Eco91I GGTNACC 1 cut(s) 632
EcoICRI GAGCTC 1 cut(s) 286
EcoNI CCTNNNNNAGG 1 cut(s) 906
EcoO109I RGGNCCY 1 cut(s) 226
EcoO65I GGTNACC 1 cut(s) 632
EcoRI GAATTC 1 cut(s) 544
EcoRII CCWGG 1 cut(s) 676
EcoT14I CCWWGG 1 cut(s) 219
EcoT38I GRGCYC 1 cut(s) 288
ErhI CCWWGG 1 cut(s) 219
FaeI CATG 3 cut(s) 40, 878, 937
FaqI GGGAC 1 cut(s) 901
FatI CATG 3 cut(s) 36, 874, 933
FbaI TGATCA 1 cut(s) 867
Fnu4HI GCNGC 9 cut(s) 53, 297, 300, 303, 342, 396, 453, 540, 828
FokI GGATG 2 cut(s) 202, 248
FriOI GRGCYC 1 cut(s) 288
Fsp4HI GCNGC 9 cut(s) 53, 297, 300, 303, 342, 396, 453, 540, 828
FspBI CTAG 2 cut(s) 653, 1030
GluI GCNGC 9 cut(s) 53, 297, 300, 303, 342, 396, 453, 540, 828
HaeIII GGCC 3 cut(s) 227, 808, 901
HapII CCGG 1 cut(s) 885
Hin1II CATG 3 cut(s) 40, 878, 937
HincII GTYRAC 1 cut(s) 477
HindII GTYRAC 1 cut(s) 477
HinfI GANTC 1 cut(s) 889
HpaII CCGG 1 cut(s) 885
HphI GGTGA 6 cut(s) 250, 559, 582, 666, 971, 1012
Hpy166II GTNNAC 4 cut(s) 95, 385, 477, 669
Hpy188I TCNGA 3 cut(s) 438, 796, 894
Hpy188III TCNNGA 3 cut(s) 281, 755, 952
Hpy8I GTNNAC 4 cut(s) 95, 385, 477, 669
HpyAV CCTTC 5 cut(s) 43, 180, 287, 525, 580
HpyCH4III ACNGT 1 cut(s) 66
HpyCH4V TGCA 6 cut(s) 95, 331, 398, 815, 830, 845
HpyF10VI GCNNNNNNNGC 4 cut(s) 302, 328, 401, 449
HpyF3I CTNAG 1 cut(s) 574
Hsp92II CATG 3 cut(s) 40, 878, 937
KpnI GGTACC 1 cut(s) 712
Ksp22I TGATCA 1 cut(s) 867
Kzo9I GATC 3 cut(s) 160, 587, 867
LmnI GCTCC 2 cut(s) 46, 60
Lsp1109I GCAGC 6 cut(s) 39, 311, 314, 382, 526, 814
LweI GCATC 6 cut(s) 60, 283, 382, 526, 715, 814
MaeI CTAG 2 cut(s) 653, 1030
MaeIII GTNAC 4 cut(s) 256, 445, 632, 959
MalI GATC 3 cut(s) 162, 589, 869
MbiI CCGCTC 1 cut(s) 801
MboI GATC 3 cut(s) 160, 587, 867
MboII GAAGA 6 cut(s) 404, 407, 892, 907, 979, 982
MhlI GDGCHC 3 cut(s) 97, 288, 772
MlsI TGGCCA 1 cut(s) 808
MluCI AATT 7 cut(s) 133, 180, 310, 353, 544, 561, 862
MluNI TGGCCA 1 cut(s) 808
MlyI GAGTC 1 cut(s) 883
MmeI TCCRAC 1 cut(s) 714
MnlI CCTC 9 cut(s) 186, 217, 275, 394, 522, 728, 891, 902, 959
Mox20I TGGCCA 1 cut(s) 808
MscI TGGCCA 1 cut(s) 808
MseI TTAA 1 cut(s) 231
MslI CAYNNNNRTG 1 cut(s) 390
Msp20I TGGCCA 1 cut(s) 808
MspI CCGG 1 cut(s) 885
MspR9I CCNGG 2 cut(s) 678, 886
Mva1269I GAATGC 1 cut(s) 527
MvaI CCWGG 1 cut(s) 678
MvnI CGCG 2 cut(s) 341, 443
MwoI GCNNNNNNNGC 4 cut(s) 302, 328, 401, 449
NciI CCSGG 1 cut(s) 886
NdeII GATC 3 cut(s) 160, 587, 867
NlaIII CATG 3 cut(s) 40, 878, 937
NlaIV GGNNCC 4 cut(s) 42, 317, 710, 771
NmuCI GTSAC 2 cut(s) 256, 959
NspI RCATGY 1 cut(s) 878
PceI AGGCCT 1 cut(s) 901
PctI GAATGC 1 cut(s) 527
PkrI GCNGC 9 cut(s) 54, 298, 301, 304, 343, 397, 454, 541, 829
PleI GAGTC 1 cut(s) 883
PpsI GAGTC 1 cut(s) 883
Psp124BI GAGCTC 1 cut(s) 288
Psp6I CCWGG 1 cut(s) 676
PspEI GGTNACC 1 cut(s) 632
PspGI CCWGG 1 cut(s) 676
PspN4I GGNNCC 4 cut(s) 42, 317, 710, 771
PspPI GGNCC 2 cut(s) 226, 316
PstI CTGCAG 2 cut(s) 400, 817
RsaI GTAC 7 cut(s) 63, 167, 362, 384, 690, 710, 1013
RsaNI GTAC 7 cut(s) 62, 166, 361, 383, 689, 709, 1012
RseI CAYNNNNRTG 1 cut(s) 390
SacI GAGCTC 1 cut(s) 288
SaqAI TTAA 1 cut(s) 231
SatI GCNGC 9 cut(s) 53, 297, 300, 303, 342, 396, 453, 540, 828
Sau3AI GATC 3 cut(s) 160, 587, 867
Sau96I GGNCC 2 cut(s) 226, 316
SchI GAGTC 1 cut(s) 883
ScrFI CCNGG 2 cut(s) 678, 886
SduI GDGCHC 3 cut(s) 97, 288, 772
SfaNI GCATC 6 cut(s) 60, 283, 382, 526, 715, 814
SfcI CTRYAG 3 cut(s) 396, 813, 856
SinI GGWCC 1 cut(s) 316
SmiMI CAYNNNNRTG 1 cut(s) 390
SmlI CTYRAG 1 cut(s) 950
SmoI CTYRAG 1 cut(s) 950
Sse9I AATT 7 cut(s) 133, 180, 310, 353, 544, 561, 862
SseBI AGGCCT 1 cut(s) 901
SsiI CCGC 5 cut(s) 296, 341, 443, 453, 801
SspMI CTAG 2 cut(s) 653, 1030
SstI GAGCTC 1 cut(s) 288
StuI AGGCCT 1 cut(s) 901
StyD4I CCNGG 2 cut(s) 676, 884
StyI CCWWGG 1 cut(s) 219
TaaI ACNGT 1 cut(s) 66
TaqI TCGA 1 cut(s) 915
TasI AATT 7 cut(s) 133, 180, 310, 353, 544, 561, 862
TatI WGTACW 4 cut(s) 61, 165, 360, 382
TauI GCSGC 3 cut(s) 299, 344, 455
Tru1I TTAA 1 cut(s) 231
Tru9I TTAA 1 cut(s) 231
TscAI CASTG 1 cut(s) 295
TseFI GTSAC 2 cut(s) 256, 959
TseI GCWGC 6 cut(s) 52, 299, 302, 395, 539, 827
Tsp45I GTSAC 2 cut(s) 256, 959
TspDTI ATGAA 4 cut(s) 25, 393, 537, 957
TspRI CASTG 1 cut(s) 295
VneI GTGCAC 1 cut(s) 93
VpaK11BI GGWCC 1 cut(s) 316
XagI CCTNNNNNAGG 1 cut(s) 906
XapI RAATTY 2 cut(s) 353, 544
XceI RCATGY 1 cut(s) 878
XcmI CCANNNNNNNNNTGG 1 cut(s) 1022
XspI CTAG 2 cut(s) 653, 1030
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.