Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb3

Orthogroup ID

OG0005756

Physical Location & Seq

Genomic Coordinates Forward (+)

22103027 .. 22117674

Gene Structure 14648 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_3g29000.t4

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4092 bp

ATGGGATCGTTGAAGAGCGAAGAGGAGCTGGAGCACGTAGAACCCATTTTGTACGTCAACGGAATTCGTAGAGTCTTGCCTGATGGATTGGCTCACCTTACTCTTCTTGAGTACCTCAGAGATATAGGTCTCACAGGGACAAAGCTTGGTTGTGGTGAAGGTGGTTGTGGAGCTTGTACTGTTATGGTTTCACAATATGACAAAAAACTGAAGAAATGCCAGCACTATGCCGTTAATGCATGCTTGGCTCCACTGTATTCTTTAGAAGGGATGCACGTAATTACAGTGGAAGGACTTGGGAACCACAAACAAGGCCTGCACCCAATTCAAGCATCATTGGCACAATCTCATGGCTCACAATGTGGATTTTGCACACCGGGTTTTGTTATGTCTGTCTATGCATTATTAAGGTCGAGTCAGACCCCACCTAATGAGGAGCAGATTGAAGAGTGTTTAGCAGGAAACTTGTGTCGATGCACTGGTTACAGACCAATTGTTGATGCTTTTCGTGTCTTTGCCAAAACAGATGATAAACCGTACATTGACATATCTTCTCTAAGCCTTGAAGGACGTAAATTTGTGTGCCCTTCAACTGGTAAGCCCTGTTCCTGTGGATTAAAAAGTGAGATATCAAGCAACCATCAGAAAACGGGTACTTGTGACACGAGGTATGCACCTGTCTCATATAGTGAAGTTGATGGAAGCACATACACAGACAAGGAATTTATTTTTCCTCCTGAGCTGGTATTGAGAAAATCAACTTATTTGAATTTGAATGGATTCAGTGGGTTAAAATGGTTTAGACCATTGAGACTTAAGCAAGTACTAGAGTTAAAAGAAAAGTACCCAGATGCAAAGTTATTGGTAGGTAATACTGAGGTAGGAATTGAAATGAGGTTGAAAAAAATTCAATATAGGGTTCTGATTTCTGTTACACATGTACCTGAACTCAGTATATTGAATGTGAAGGATGATGGAATTGAGATAGGTTCTGTTGTAAGACTTTCTGAACTACTTAAAGTCTTGAGAAAGGTCATAACAGAACGTGCAGCTCATGAAACTTCTTCCTGTAAGGCTTTTGTTGAACAATTAAAATGGTTTGCGGGGATGCAAATAAGAAATGTTGCCTGTGTGGGTGGAAATATCTGTACAGCCAGTCCAATTTCAGACTTGAACCCCCTCTGGATGGCTGCCAGGGCCAAGTTTCAAATTATTGATGCCAAAGGGAACATTAGAACAACCCCAGCAGAGAATTTTTTCCTCAGCTACCGTAAGGTGGATCTGGGAAGTGGTGAAATTCTACTATCTGTATTTTTACCTTGGACTAAGCCGTTTGAGTATGTGAAAGAATATAAACAAGCTCACAGAAGGGATGATGATATTGCAATTGTGAATGCTGGAATTCGTGTTCATCTTGAGGAAAGAGGTGAAGACATTGTTGTTTCTGATGCATCTATTGTTTATGGGGGTGTAGCTCCACTCTCTCTTTCTGCTACAAGAACAAAAGACTTCCTCATTGGAAAGATTTGGAACCAGGAGCTATTGCAGGGTGCTCTTAAAGTCCTCCAGAAGGATGTGATACTCAGAGATAATGCTCCTGGTGGGATGGTGGAGTTCCGTAAGTCTTTGACAGCTAGCTTTTTCTTCAAGTTTTTTCTGTGGGTTTCTCATCAGCTGGACAGGGAAAAAGGCCTTAAGGGGAGTGTACCGTTATCTCATCTTTCTGCTATACAACCGTTTCACAGGCCATCTGTTATAGGAACTCAAGACTATGAAATTACAAAACATGGGACAGCTGTTGGGTCTCCTGAGGTTCATCTGTCGGCGAAACTTCAGGTTTCAGGAGAGGCAGAATATGCTGATGATACACCATTGCCTCCAAATGGTTTGCATGCTGCTTTAGTATTGAGCAAAAAACCTCATGCTCGTATTCTTTCAATCGATGATTCAGGAGCAAAGATGTCACCTGGGTTTGCAGGCGTATTTTTTGCCAAGGATGTTCCAGCTGATAATAAAATTGGACCAGTTGTTGCTGATGAGGAACTATTTGCTTCAGAGTATGTCACTTGTGTAGGTCAGGTTATAGGAGTGGTAGTTGCAGATACACATGAAAAGGCAAAATTAGCAGCAACAAAAGTTCATGTTGAGTATGAAGAGCTCCCAGCTATCTTATCAATACAGGATGCTATCAATGCTAATAGTTTCCATCCTAATACTGAGAGGTGTTTTAGGAAAGGAGATGTGGATCTTTGCTTTCAATCAGGTCAATGTGACAAGGTCATAGAGGGGGAAGTTCTAGTGGGAGGCCAGGAACACTTCTATTTGGAGCCACATAGCAGTGTGATATGGACAATGGATGGTGGCAATGAAGTTCATATGATCTCATCTACTCAAGCTCCTCAGAAGCACCAGAAGTACGTATCTCATGTTCTTGGTCTTCCAATGTCAAAAGTAGTTTGTAAAACAAAGCGTATTGGTGGTGGTTTTGGCGGAAAGGAGACAAGATCATGTTTCATTGCTGCTGCAGCTTCTGTTCCTTCATTTCTATTAAATCGACCAGTCAAAATTACATTAGACCGTGATACAGACATGATGATAACTGGGCAGCGCCATAGCTTTCTTGGAAAATACAAGGTGGGCTTCACGAATGAGGGAAAGGTATTGGCATTGGATCTTCACATCTACAATAGTGCTGGAAATTCTCTAGACTTGTCCCTTCCCGTTCTTGAGCGTGCTATGTTTCACTCAGATAATGTCTATGAGATTCCAAATGTGAGAATTGTGGGAAGAGTTTGCTTCACTAATATACCCAGTAACACTGCTTTCCGAGGATTTGGTGGTCCTCAAGGGATGATTATTGCTGAAAACTGGATCCAAAGAATTGCTGTAGAACAGAAGAAAAGCCCAGAAGAGATAAGAGAAATTAATTTTCAAGGTGAAGGGTCGATATTGCATTATGGTCAACAACTTGAACATTGTACACTGGCCCCGCTTTGGAACGAACTAAAACTATCTTGTGAATTTTCAAAGGCTCGCAATGAAGTTCTCCAGTATAATACTCGTAATCGGTGGCGGAAGCGTGGTGTAGCCATGATACCAACAAAGTTTGGCATATCATTTACATTAAAGCTCATGAACCAGGCAGGTGCTCTTGTTCATGTCTACACAGATGGAACTGTTCTAGTCTCACATGGAGGTGTTGAGATGGGGCAAGGTCTGCATACAAAAGTTGCTCAGGTGGCTGCTTCTGCCTTCAATATTCCTCTCAGTTCTGTCTTCATATCAGAGACAAGTACCGACAAGGTGCCTAATGCTTCCCCAACAGCAGCTTCTGCAAGTTCTGACATGTATGGAGCTGCAGTCTTGGATGCATGTGAGCAAATCAAGGCACGCATGGAACCTATTGCTTCACAACACAATTTCAGCTCTTTTGCTGAGCTGGCAAGTGCATGCTATGTGGCCCGGATAGATCTTTCTGCCCATGGATTTTACATTATACCTGAGATCGACTTTGATTGGACTACTGGTAAAGGCACACCATTCAGGTACTTTACCTATGGGGCTGCTTTTGCCGAGGTTGAAATCGACACTTTGACTGGAGATTTCCACACTAGGGTGGCAAACATATTCCTGGATCTTGGATACTCACTCAACCCAGCAGTTGACGTGGGGCAGATCGAAGGAGCGTTTATACAAGGTTTGGGTTGGGTCGCCCTTGAAGAACTAAAATGGGGAGATCCTGCTCATAAGTGGATTGCACCAGGTTCCCTCTACACTTGTGGGCCTGGAAGCTACAAAATTCCTTCCATAAATGACGTTCCCTTCAAGTTCAATGTTTCACTTCTGAAGGGACATCCTAATGTAAAGGCCATCCACTCATCTAAAGCTGTTGGTGAGCCCCCATTTTTCTTGGCATCAGCGGTGTTTTTTGCCATCAAAGATGCCATTATAGCTGCGAGAGCTGACGTAGGGTGTAATGAGTGGTTTCCACTTGATAATCCAGCAACACCAGAACGTATACGGATGGCTTGTTTCGATGAATTTACATCAGCATTTGCTAGCTCGGATTTTCGCGCAAATCTTAGTGTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0000302 GO:0001101 GO:0003674 GO:0003824 GO:0004854 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006139 GO:0006144 GO:0006145 GO:0006725 GO:0006801 GO:0006807 GO:0006950 GO:0006979 GO:0008150 GO:0008152 GO:0009056 GO:0009112 GO:0009115 GO:0009414 GO:0009415 GO:0009628 GO:0009987 GO:0010035 GO:0016491 GO:0016725 GO:0016726 GO:0016903 GO:0019439 GO:0034641 GO:0036094 GO:0042221 GO:0042554 GO:0043167 GO:0043168 GO:0043546 GO:0044237 GO:0044238 GO:0044248 GO:0044270 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046110 GO:0046113 GO:0046483 GO:0046700 GO:0048037 GO:0050660 GO:0050662 GO:0050896 GO:0051536 GO:0051537 GO:0051540 GO:0055086 GO:0055114 GO:0071704 GO:0072521 GO:0072523 GO:0072593 GO:0097159 GO:1901265 GO:1901360 GO:1901361 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00230 ko00232 ko00983 ko01100 ko01110 ko01120 ko04146 map00230 map00232 map00983 map01100 map01110 map01120 map04146

Pfam Domains

Protein Families

Ald_Xan_dh_C Ald_Xan_dh_C2 CO_deh_flav_C FAD_binding_5 Fer2 Fer2_2

Protein Analysis

1364

Amino Acids

149.68

Weight (kDa)

6.61

Isoelectric Point (pI)

35.79

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Fer2	PF00111	32 - 84	3.6e-08	2Fe-2S iron-sulfur cluster binding domain
Fer2_2	PF01799	95 - 169	4.5e-32	[2Fe-2S] binding domain
FAD_binding_5	PF00941	264 - 441	2e-49	FAD binding domain in molybdopterin dehydrogenase
CO_deh_flav_C	PF03450	450 - 555	2.5e-29	CO dehydrogenase flavoprotein C-terminal domain
Ald_Xan_dh_C	PF01315	614 - 721	7.8e-30	Aldehyde oxidase and xanthine dehydrogenase, a/b hammerhead domain
MoCoBD_1	PF02738	739 - 977	5.1e-85	Molybdopterin cofactor-binding domain
MoCoBD_2	PF20256	1005 - 1138	1.1e-45	Molybdopterin cofactor-binding domain
MoCoBD_2	PF20256	1147 - 1195	6.7e-07	Molybdopterin cofactor-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0005756)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G34890 AT4G34900 AT4G34900 AT4G34900
fragaria_vesca	FvH4_3g29000 FvH4_3g29000 FvH4_3g29000 FvH4_3g29000
malus_domestica	MD03G1177200.v1.1 MD11G1161000.v1.1 MD11G1197900.v1.1
prunus_persica	Prupe.4G245700_v2.0.a1 Prupe.4G245700_v2.0.a1
pyrus_communis	pycom11g13410 pycom11g17110 pycom11g17120
rosa_chinensis	RchiOBHm_Chr5g0051661
rosa_laevigata	RLG00000034866
rosa_multiflora	Rmu_sc0000068.1_g000026 Rmu_sc0014882.1_g000001 Rmu_sc0018333.1_g000002
rosa_roxburghii	Rroxscaffold_1G00027170
rosa_rugosa	Rorug05G0277900
rosa_samantha	Rh5AG349700 Rh5BG361400 Rh5CG385900 Rh5DG376200
rosa_wichuraiana	Rw5G033070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	3134
Acc36I	ACCTGC	1 cut(s)	3134
AccB1I	GGYRCC	1 cut(s)	3304
AccI	GTMKAC	2 cut(s)	3162, 4018
AccII	CGCG	1 cut(s)	4074
AciI	CCGC	5 cut(s)	1103, 2490, 2990, 3073, 3920
AclWI	GGATC	8 cut(s)	13, 1287, 2253, 2679, 2866, 2879, 3642, 3731
AcuI	CTGAAG	4 cut(s)	230, 1817, 2037, 3866
AdeI	CACNNNGTG	1 cut(s)	362
AfiI	CCNNNNNNNGG	7 cut(s)	593, 1186, 1276, 1570, 1883, 2994, 3613
AflII	CTTAAG	2 cut(s)	815, 1694
AflIII	ACRYGT	2 cut(s)	937, 3345
AjiI	CACGTC	1 cut(s)	3667
AjnI	CCWGG	9 cut(s)	1193, 1533, 1597, 1966, 2307, 3138, 3630, 3760, 3784
AjuI	GAANNNNNNNTTGG	2 cut(s)	3313, 3345
AleI	CACNNNNGTG	1 cut(s)	3614
AloI	GAACNNNNNNTCC	2 cut(s)	1392, 1424
Alw21I	GWGCWC	4 cut(s)	36, 1555, 2160, 3151
Alw26I	GTCTC	7 cut(s)	134, 685, 805, 1809, 2492, 3190, 3281
AlwI	GGATC	8 cut(s)	13, 1287, 2253, 2679, 2866, 2879, 3642, 3731
AlwNI	CAGNNNCTG	2 cut(s)	2531, 3332
AoxI	GGCC	9 cut(s)	313, 1197, 1690, 1745, 2305, 2985, 3459, 3782, 3867
AseI	ATTAAT	1 cut(s)	2925
Asp700I	GAANNNNTTC	2 cut(s)	779, 1256
AspLEI	GCGC	2 cut(s)	2610, 4076
AspS9I	GGNCC	6 cut(s)	1197, 2021, 2840, 2986, 3460, 3782
AsuC2I	CCSGG	2 cut(s)	378, 3463
AsuHPI	GGTGA	7 cut(s)	86, 167, 1304, 1439, 1956, 2948, 3905
AsuNHI	GCTAGC	2 cut(s)	1634, 4058
AvaII	GGWCC	2 cut(s)	2021, 2840
AxyI	CCTNAGG	1 cut(s)	1809
BaeGI	GKGCMC	1 cut(s)	587
BaeI	ACNNNNGTAYC	2 cut(s)	924, 957
BamHI	GGATCC	1 cut(s)	2871
BanI	GGYRCC	1 cut(s)	3304
BanII	GRGCYC	2 cut(s)	2160, 3900
BauI	CACGAG	1 cut(s)	664
BbsI	GAAGAC	3 cut(s)	1437, 2429, 3268
Bbv12I	GWGCWC	4 cut(s)	36, 1555, 2160, 3151
BbvCI	CCTCAGC	1 cut(s)	1262
BceAI	ACGGC	2 cut(s)	215, 1315
BciT130I	CCWGG	9 cut(s)	1195, 1535, 1599, 1968, 2309, 3140, 3632, 3762, 3786
BciVI	GTATCC	1 cut(s)	3635
BcnI	CCSGG	2 cut(s)	378, 3463
BcoDI	GTCTC	7 cut(s)	134, 685, 805, 1809, 2492, 3190, 3281
BfaI	CTAG	7 cut(s)	827, 1635, 2297, 2705, 3182, 3612, 4059
BfmI	CTRYAG	3 cut(s)	2523, 2886, 3357
BfoI	RGCGCY	1 cut(s)	2611
BfrI	CTTAAG	2 cut(s)	815, 1694
BfuAI	ACCTGC	1 cut(s)	3134
BfuI	GTATCC	1 cut(s)	3635
BglII	AGATCT	1 cut(s)	3469
BlpI	GCTNAGC	1 cut(s)	3435
BmcAI	AGTACT	1 cut(s)	825
Bme18I	GGWCC	2 cut(s)	2021, 2840
BmgBI	CACGTC	1 cut(s)	3667
BmgT120I	GGNCC	6 cut(s)	1197, 2021, 2840, 2986, 3460, 3782
BmiI	GGNNCC	9 cut(s)	249, 302, 1532, 2328, 2873, 2988, 3306, 3399, 3766
BmrI	ACTGGG	2 cut(s)	2610, 2805
BmtI	GCTAGC	2 cut(s)	1638, 4062
BmuI	ACTGGG	2 cut(s)	2610, 2805
BpiI	GAAGAC	3 cut(s)	1437, 2429, 3268
BpmI	CTGGAG	4 cut(s)	50, 1550, 3032, 3618
Bpu10I	CCTNAGC	3 cut(s)	738, 1262, 3234
Bpu1102I	GCTNAGC	1 cut(s)	3435
BpuEI	CTTGAG	7 cut(s)	128, 1045, 1436, 1749, 2376, 2747, 2829
BpuMI	CCSGG	2 cut(s)	378, 3463
Bsa29I	ATCGAT	1 cut(s)	1941
BsaAI	YACGTR	3 cut(s)	37, 277, 2419
BsaBI	GATNNNNATC	1 cut(s)	2244
BsaI	GGTCTC	2 cut(s)	134, 1809
BsaJI	CCNNGG	7 cut(s)	1194, 1319, 1967, 1992, 2827, 3481, 3573
BsaXI	ACNNNNNCTCC	6 cut(s)	2079, 2109, 2380, 2410, 3345, 3375
Bsc4I	CCNNNNNNNGG	7 cut(s)	593, 1186, 1276, 1570, 1883, 2994, 3613
Bse21I	CCTNAGG	1 cut(s)	1809
Bse3DI	GCAATG	4 cut(s)	1871, 2371, 2514, 3043
Bse8I	GATNNNNATC	1 cut(s)	2244
BseBI	CCWGG	9 cut(s)	1195, 1535, 1599, 1968, 2309, 3140, 3632, 3762, 3786
BseCI	ATCGAT	1 cut(s)	1941
BseDI	CCNNGG	7 cut(s)	1194, 1319, 1967, 1992, 2827, 3481, 3573
BseJI	GATNNNNATC	1 cut(s)	2244
BseLI	CCNNNNNNNGG	7 cut(s)	593, 1186, 1276, 1570, 1883, 2994, 3613
BseMI	GCAATG	4 cut(s)	1871, 2371, 2514, 3043
BseRI	GAGGAG	3 cut(s)	38, 449, 2388
BseSI	GKGCMC	1 cut(s)	587
BseYI	CCCAGC	3 cut(s)	1243, 2161, 3655
BsgI	GTGCAG	2 cut(s)	302, 1068
Bsh1236I	CGCG	1 cut(s)	4074
BshFI	GGCC	9 cut(s)	315, 1199, 1692, 1747, 2307, 2987, 3461, 3784, 3869
BshNI	GGYRCC	1 cut(s)	3304
BshVI	ATCGAT	1 cut(s)	1941
BsiHKAI	GWGCWC	4 cut(s)	36, 1555, 2160, 3151
BsiSI	CCGG	2 cut(s)	377, 3463
BslFI	GGGAC	4 cut(s)	151, 1804, 2698, 3864
BslI	CCNNNNNNNGG	7 cut(s)	593, 1186, 1276, 1570, 1883, 2994, 3613
BsmAI	GTCTC	7 cut(s)	134, 685, 805, 1809, 2492, 3190, 3281
BsmFI	GGGAC	4 cut(s)	151, 1804, 2698, 3864
BsmI	GAATGC	1 cut(s)	1399
BsnI	GGCC	9 cut(s)	315, 1199, 1692, 1747, 2307, 2987, 3461, 3784, 3869
Bso31I	GGTCTC	2 cut(s)	134, 1809
Bsp1286I	GDGCHC	6 cut(s)	36, 587, 1555, 2160, 3151, 3900
Bsp1407I	TGTACA	2 cut(s)	1148, 2978
Bsp1720I	GCTNAGC	1 cut(s)	3435
Bsp19I	CCATGG	1 cut(s)	3481
BspACI	CCGC	5 cut(s)	1103, 2490, 2990, 3073, 3920
BspANI	GGCC	9 cut(s)	315, 1199, 1692, 1747, 2307, 2987, 3461, 3784, 3869
BspDI	ATCGAT	1 cut(s)	1941
BspFNI	CGCG	1 cut(s)	4074
BspHI	TCATGA	2 cut(s)	1054, 3132
BspLI	GGNNCC	9 cut(s)	249, 302, 1532, 2328, 2873, 2988, 3306, 3399, 3766
BspMAI	CTGCAG	2 cut(s)	2527, 3361
BspMI	ACCTGC	1 cut(s)	3134
BspOI	GCTAGC	2 cut(s)	1638, 4062
BspPI	GGATC	8 cut(s)	13, 1287, 2253, 2679, 2866, 2879, 3642, 3731
BspQI	GCTCTTC	2 cut(s)	8, 2148
BspT107I	GGYRCC	1 cut(s)	3304
BspTI	CTTAAG	2 cut(s)	815, 1694
BspTNI	GGTCTC	2 cut(s)	134, 1809
BsrDI	GCAATG	4 cut(s)	1871, 2371, 2514, 3043
BsrGI	TGTACA	2 cut(s)	1148, 2978
BssECI	CCNNGG	7 cut(s)	1194, 1319, 1967, 1992, 2827, 3481, 3573
BssNAI	GTATAC	1 cut(s)	4019
BssSI	CACGAG	1 cut(s)	664
BssT1I	CCWWGG	3 cut(s)	1319, 1992, 3481
Bst1107I	GTATAC	1 cut(s)	4019
Bst2BI	CACGAG	1 cut(s)	664
Bst2UI	CCWGG	9 cut(s)	1195, 1535, 1599, 1968, 2309, 3140, 3632, 3762, 3786
Bst4CI	ACNGT	9 cut(s)	181, 255, 286, 537, 1271, 1710, 1737, 2579, 3178
Bst6I	CTCTTC	7 cut(s)	8, 15, 108, 441, 2148, 2782, 2904
BstAFI	CTTAAG	2 cut(s)	815, 1694
BstAPI	GCANNNNNTGC	3 cut(s)	1856, 3217, 3332
BstAUI	TGTACA	2 cut(s)	1148, 2978
BstBAI	YACGTR	3 cut(s)	37, 277, 2419
BstDSI	CCRYGG	1 cut(s)	3481
BstENI	CCTNNNNNAGG	1 cut(s)	1568
BstFNI	CGCG	1 cut(s)	4074
BstH2I	RGCGCY	1 cut(s)	2611
BstHHI	GCGC	2 cut(s)	2610, 4076
BstMAI	GTCTC	7 cut(s)	134, 685, 805, 1809, 2492, 3190, 3281
BstNI	CCWGG	9 cut(s)	1195, 1535, 1599, 1968, 2309, 3140, 3632, 3762, 3786
BstNSI	RCATGY	6 cut(s)	243, 941, 1895, 3349, 3375, 3453
BstSFI	CTRYAG	3 cut(s)	2523, 2886, 3357
BstSLI	GKGCMC	1 cut(s)	587
BstSNI	TACGTA	1 cut(s)	2419
BstUI	CGCG	1 cut(s)	4074
BstV2I	GAAGAC	3 cut(s)	1437, 2429, 3268
BstX2I	RGATCY	7 cut(s)	1279, 2245, 2671, 2871, 3469, 3634, 3736
BstYI	RGATCY	7 cut(s)	1279, 2245, 2671, 2871, 3469, 3634, 3736
BstZ17I	GTATAC	1 cut(s)	4019
Bsu15I	ATCGAT	1 cut(s)	1941
Bsu36I	CCTNAGG	1 cut(s)	1809
BsuI	GTATCC	1 cut(s)	3635
BsuRI	GGCC	9 cut(s)	315, 1199, 1692, 1747, 2307, 2987, 3461, 3784, 3869
BsuTUI	ATCGAT	1 cut(s)	1941
BtgI	CCRYGG	1 cut(s)	3481
BtrI	CACGTC	1 cut(s)	3667
BtsI	GCAGTG	2 cut(s)	2344, 2817
BtsIMutI	CAGTG	7 cut(s)	251, 291, 477, 790, 2344, 2817, 2981
BveI	ACCTGC	1 cut(s)	3134
CaiI	CAGNNNCTG	2 cut(s)	2531, 3332
CciI	TCATGA	2 cut(s)	1054, 3132
CfoI	GCGC	2 cut(s)	2610, 4076
Cfr13I	GGNCC	6 cut(s)	1197, 2021, 2840, 2986, 3460, 3782
ClaI	ATCGAT	1 cut(s)	1941
CsiI	ACCWGGT	1 cut(s)	3760
DraIII	CACNNNGTG	1 cut(s)	362
Eam1104I	CTCTTC	7 cut(s)	8, 15, 108, 441, 2148, 2782, 2904
EarI	CTCTTC	7 cut(s)	8, 15, 108, 441, 2148, 2782, 2904
EciI	GGCGGA	2 cut(s)	2505, 3088
Ecl136II	GAGCTC	1 cut(s)	2158
Eco105I	TACGTA	1 cut(s)	2419
Eco130I	CCWWGG	3 cut(s)	1319, 1992, 3481
Eco147I	AGGCCT	2 cut(s)	315, 1692
Eco24I	GRGCYC	2 cut(s)	2160, 3900
Eco31I	GGTCTC	2 cut(s)	134, 1809
Eco32I	GATATC	1 cut(s)	630
Eco47I	GGWCC	2 cut(s)	2021, 2840
Eco53kI	GAGCTC	1 cut(s)	2158
Eco57I	CTGAAG	4 cut(s)	230, 1817, 2037, 3866
Eco81I	CCTNAGG	1 cut(s)	1809
EcoICRI	GAGCTC	1 cut(s)	2158
EcoNI	CCTNNNNNAGG	1 cut(s)	1568
EcoRI	GAATTC	2 cut(s)	63, 1401
EcoRII	CCWGG	9 cut(s)	1193, 1533, 1597, 1966, 2307, 3138, 3630, 3760, 3784
EcoRV	GATATC	1 cut(s)	630
EcoT14I	CCWWGG	3 cut(s)	1319, 1992, 3481
EcoT22I	ATGCAT	4 cut(s)	241, 403, 1453, 3373
EcoT38I	GRGCYC	2 cut(s)	2160, 3900
ErhI	CCWWGG	3 cut(s)	1319, 1992, 3481
FalI	AAGNNNNNCTT	2 cut(s)	2624, 2656
FaqI	GGGAC	4 cut(s)	151, 1804, 2698, 3864
FauI	CCCGC	2 cut(s)	1096, 2997
FauNDI	CATATG	1 cut(s)	2376
FblI	GTMKAC	2 cut(s)	3162, 4018
FriOI	GRGCYC	2 cut(s)	2160, 3900
FspBI	CTAG	7 cut(s)	827, 1635, 2297, 2705, 3182, 3612, 4059
GlaI	GCGC	2 cut(s)	2609, 4075
GsaI	CCCAGC	3 cut(s)	1247, 2165, 3659
GsuI	CTGGAG	4 cut(s)	50, 1550, 3032, 3618
HaeII	RGCGCY	1 cut(s)	2611
HaeIII	GGCC	9 cut(s)	315, 1199, 1692, 1747, 2307, 2987, 3461, 3784, 3869
HapII	CCGG	2 cut(s)	377, 3463
HhaI	GCGC	2 cut(s)	2610, 4076
Hin6I	GCGC	2 cut(s)	2608, 4074
HinP1I	GCGC	2 cut(s)	2608, 4074
HincII	GTYRAC	3 cut(s)	58, 2963, 3664
HindII	GTYRAC	3 cut(s)	58, 2963, 3664
HindIII	AAGCTT	1 cut(s)	143
HinfI	GANTC	5 cut(s)	72, 415, 780, 1946, 2764
HpaII	CCGG	2 cut(s)	377, 3463
HphI	GGTGA	7 cut(s)	86, 167, 1304, 1439, 1956, 2948, 3905
Hpy166II	GTNNAC	7 cut(s)	58, 1706, 2963, 2981, 3163, 3664, 4019
Hpy8I	GTNNAC	7 cut(s)	58, 1706, 2963, 2981, 3163, 3664, 4019
HpyCH4III	ACNGT	9 cut(s)	181, 255, 286, 537, 1271, 1710, 1737, 2579, 3178
HspAI	GCGC	2 cut(s)	2608, 4074
LguI	GCTCTTC	2 cut(s)	8, 2148
MabI	ACCWGGT	1 cut(s)	3760
MaeI	CTAG	7 cut(s)	827, 1635, 2297, 2705, 3182, 3612, 4059
MaeIII	GTNAC	7 cut(s)	482, 659, 931, 1962, 2062, 2270, 2813
MfeI	CAATTG	2 cut(s)	492, 1386
MflI	RGATCY	7 cut(s)	1279, 2245, 2671, 2871, 3469, 3634, 3736
MhlI	GDGCHC	6 cut(s)	36, 587, 1555, 2160, 3151, 3900
MlyI	GAGTC	2 cut(s)	81, 424
Mph1103I	ATGCAT	4 cut(s)	241, 403, 1453, 3373
MroXI	GAANNNNTTC	2 cut(s)	779, 1256
MslI	CAYNNNNRTG	4 cut(s)	2337, 3195, 3614, 3858
MspA1I	CMGCKG	4 cut(s)	1675, 1796, 2006, 3920
MspCI	CTTAAG	2 cut(s)	815, 1694
MspI	CCGG	2 cut(s)	377, 3463
MunI	CAATTG	2 cut(s)	492, 1386
Mva1269I	GAATGC	1 cut(s)	1399
MvaI	CCWGG	9 cut(s)	1195, 1535, 1599, 1968, 2309, 3140, 3632, 3762, 3786
MvnI	CGCG	1 cut(s)	4074
NciI	CCSGG	2 cut(s)	378, 3463
NcoI	CCATGG	1 cut(s)	3481
NdeI	CATATG	1 cut(s)	2376
NheI	GCTAGC	2 cut(s)	1634, 4058
NlaIV	GGNNCC	9 cut(s)	249, 302, 1532, 2328, 2873, 2988, 3306, 3399, 3766
NmeAIII	GCCGAG	1 cut(s)	3598
NmuCI	GTSAC	4 cut(s)	659, 1962, 2062, 2270
NsiI	ATGCAT	4 cut(s)	241, 403, 1453, 3373
NspI	RCATGY	6 cut(s)	243, 941, 1895, 3349, 3375, 3453
OliI	CACNNNNGTG	1 cut(s)	3614
PaeI	GCATGC	3 cut(s)	243, 1895, 3453
PagI	TCATGA	2 cut(s)	1054, 3132
PaqCI	CACCTGC	1 cut(s)	3134
PceI	AGGCCT	2 cut(s)	315, 1692
PciI	ACATGT	2 cut(s)	937, 3345
PciSI	GCTCTTC	2 cut(s)	8, 2148
PctI	GAATGC	1 cut(s)	1399
PdmI	GAANNNNTTC	2 cut(s)	779, 1256
PfeI	GAWTC	3 cut(s)	780, 1946, 2764
PflFI	GACNNNGTC	1 cut(s)	2276
PfoI	TCCNGGA	1 cut(s)	3630
PleI	GAGTC	2 cut(s)	80, 423
PpsI	GAGTC	2 cut(s)	80, 423
Ppu21I	YACGTR	3 cut(s)	37, 277, 2419
PscI	ACATGT	2 cut(s)	937, 3345
PshBI	ATTAAT	1 cut(s)	2925
Psp124BI	GAGCTC	1 cut(s)	2160
Psp6I	CCWGG	9 cut(s)	1193, 1533, 1597, 1966, 2307, 3138, 3630, 3760, 3784
PspFI	CCCAGC	3 cut(s)	1243, 2161, 3655
PspGI	CCWGG	9 cut(s)	1193, 1533, 1597, 1966, 2307, 3138, 3630, 3760, 3784
PspN4I	GGNNCC	9 cut(s)	249, 302, 1532, 2328, 2873, 2988, 3306, 3399, 3766
PspPI	GGNCC	6 cut(s)	1197, 2021, 2840, 2986, 3460, 3782
PsrI	GAACNNNNNNTAC	4 cut(s)	589, 621, 2412, 2444
PstI	CTGCAG	2 cut(s)	2527, 3361
PstNI	CAGNNNCTG	2 cut(s)	2531, 3332
PsuI	RGATCY	7 cut(s)	1279, 2245, 2671, 2871, 3469, 3634, 3736
PsyI	GACNNNGTC	1 cut(s)	2276
PvuII	CAGCTG	3 cut(s)	1675, 1796, 2006
RseI	CAYNNNNRTG	4 cut(s)	2337, 3195, 3614, 3858
SacI	GAGCTC	1 cut(s)	2160
SapI	GCTCTTC	2 cut(s)	8, 2148
Sau96I	GGNCC	6 cut(s)	1197, 2021, 2840, 2986, 3460, 3782
ScaI	AGTACT	1 cut(s)	825
SchI	GAGTC	2 cut(s)	81, 424
SduI	GDGCHC	6 cut(s)	36, 587, 1555, 2160, 3151, 3900
SexAI	ACCWGGT	1 cut(s)	3760
SfcI	CTRYAG	3 cut(s)	2523, 2886, 3357
SinI	GGWCC	2 cut(s)	2021, 2840
SmiMI	CAYNNNNRTG	4 cut(s)	2337, 3195, 3614, 3858
SmlI	CTYRAG	9 cut(s)	107, 815, 1024, 1415, 1694, 1764, 2391, 2726, 2844
SmoI	CTYRAG	9 cut(s)	107, 815, 1024, 1415, 1694, 1764, 2391, 2726, 2844
SnaBI	TACGTA	1 cut(s)	2419
SphI	GCATGC	3 cut(s)	243, 1895, 3453
SseBI	AGGCCT	2 cut(s)	315, 1692
SsiI	CCGC	5 cut(s)	1103, 2490, 2990, 3073, 3920
SspI	AATATT	1 cut(s)	3259
SspMI	CTAG	7 cut(s)	827, 1635, 2297, 2705, 3182, 3612, 4059
SstI	GAGCTC	1 cut(s)	2160
StuI	AGGCCT	2 cut(s)	315, 1692
StyI	CCWWGG	3 cut(s)	1319, 1992, 3481
TaaI	ACNGT	9 cut(s)	181, 255, 286, 537, 1271, 1710, 1737, 2579, 3178
TaqI	TCGA	9 cut(s)	413, 472, 1941, 2554, 2945, 3507, 3585, 3678, 4035
TatI	WGTACW	4 cut(s)	176, 823, 1148, 2978
TfiI	GAWTC	3 cut(s)	780, 1946, 2764
TscAI	CASTG	7 cut(s)	258, 291, 484, 790, 2344, 2824, 2988
TseFI	GTSAC	4 cut(s)	659, 1962, 2062, 2270
Tsp45I	GTSAC	4 cut(s)	659, 1962, 2062, 2270
TspGWI	ACGGA	3 cut(s)	75, 1607, 4036
TspRI	CASTG	7 cut(s)	258, 291, 484, 790, 2344, 2824, 2988
Tth111I	GACNNNGTC	1 cut(s)	2276
Vha464I	CTTAAG	2 cut(s)	815, 1694
VpaK11BI	GGWCC	2 cut(s)	2021, 2840
VspI	ATTAAT	1 cut(s)	2925
XagI	CCTNNNNNAGG	1 cut(s)	1568
XbaI	TCTAGA	1 cut(s)	2704
XceI	RCATGY	6 cut(s)	243, 941, 1895, 3349, 3375, 3453
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2618
XmiI	GTMKAC	2 cut(s)	3162, 4018
XmnI	GAANNNNTTC	2 cut(s)	779, 1256
XspI	CTAG	7 cut(s)	827, 1635, 2297, 2705, 3182, 3612, 4059
ZrmI	AGTACT	1 cut(s)	825
Zsp2I	ATGCAT	4 cut(s)	241, 403, 1453, 3373

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.