Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0005756

Physical Location & Seq

Genomic Coordinates Forward (+)

28472511 .. 28484115

Gene Structure 11605 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1197900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4107 bp

ATGGGATCGTTGAAGAAGGAAGAGGAGGACCTGGAACAATCGGGGGAGGACTCAAAAGAGGCCATACTGTATGTCAATGGAGTTCGTAGAGTTTTGCCTGACGGGTTGGCCCATTTGACCCTTCTCGAGTATCTCAGAGATATAGGTTTGACGGGGACAAAGCTTGGCTGTGGTGAAGGTGGTTGTGGTGCTTGCACTGTTATGGTTTCGCATTACGACAAAGAACTGAAGAAAAGTTTCCACTATGCTGTCAACGCATGCTTGGCTTCTTTGTATTCTGTAGAAGGGATGCATGTAATTACTGTGGAAGGACTAGGGAGCCACAAGCAGGGCTTGCACCCAATTCAAGAATCATTGGCACGATCTCATGGTTCACAATGTGGATTTTGCACCCCTGGTTTTATTATGTCCATCTATGCCTTGTTAAGGTCAAGTCAGAAACCACCTAATGAGGAGCAGATTGAAGAATGTCTTGCAGGAAATTTGTGTAGATGCACAGGTTACAGGCCAATTGTTGATGCTTTTCGTGTCTTTGCCAAAACAAATGATACACCATACATTAATACATCTTCACTAAGCTCCGAAGGAGGTGAGTTTGTGTGCCCTTCGACTGGAAAGCCCTGTTCGTGTGGATTGAAAAGTGAAAGTTCATGCACCACTCATGAAAGTGGTACTCATGGTGAGAGGTATGCACCTGTCTCGTACAGTGAAATTGATGGAAGCACATACACAGACAAGGAGTTTATTTTTCCTCCTGAGCTATTGTTGAGAAAATCGACTTATTTGAGTTTGACCGGTTTTGGTGGATTAAGGTGGTTCCGACCGTTGAGGCTTAAACAAGTGCTAGGATTGAAGGAAAAATACCCTGATGCAAAATTATTGGTAGGCAATACTGAGGTGGGAATTGAAATGAGGTTGAAAAATATTCAATATAAGGTTTTGATTTTCGTCACGAATGTACCTGAACTCAGTAAATTGACTGTGAAGGATGATGGCATAGAGATAGGTTCAGCAGTAAGACTTTCTGAACTACTTAAAGTTCTAAGAACGGTCATAACAGAACGTGCAGCTCATGAAACTTCTGCTTGTAAGGCCTTCGTTGAACAATTAAAATGGTTTGCTGGGGTGCAAATAAGAAATGTTGCATCTGTGGGTGGAAATATCTGTACAGCCAGTCCAATATCAGACTTGAACCCTCTTTGGATGGCCTCTAGAGCGAAATTTCAGATTATTGATTGCAAAGGGAATATTAGAACGACACTGGCAGAAAACTTTTTCTTGGGTTACCGAAAGGTGGATCTGGCCAGTGGTGAAATTTTACTCTCTGTATTTTTACCTTGGACTAGGTCCTTCGAGTATGTGAAAGAGTATAAGCAAGCTCACAGAAGGGACGATGATATTGCAATTGTGAATGCTGGGATTCGTGTTCATCTTGAGTACAGAGGTGGCTGGGTTGTGTCTGATGCATCCATTGCTTATGGTGGAGTTGCTCCACTCTCTCTTTCTGCAAAAAGAACAAAAAAATTTCTCATTGGGAAGAGTTGGAACCAGGAGATGTTGCAGGGTGCCTTGAAAATCCTGCAAGAAGATGTATTGCTCAAGGATGATGCTCCTGGTGGAATGGTGGAGTTTCGTAGATCATTGAGTGTTAGCTTTTTCTTCAAATTTTTTCTTTGGGTTTCTCATCAAATGGAAGGGAAGCAATGCATTAAAGAGAGTGTACCATTATCTCATCTTTCTGCTGTACAATCATTTCACCGGCCACCTGTTATAGGATCTCAAGATTATGAAGTCATAAAACGTGGGACAGCTGTTGGGTCTCCCGAGGTTCATTTATCAGCAAGACTTCAGGTTACAGGAGAGGCGGAATATGCCGATGACACACCATTGCCTCCTAATTGTTTACACGCTGCTTTAATACTCAGCAGAAAGCCTCACGCTCGTATACGTTCAATCGATGATTCTGGAGCAAAGTTATCACCTGGGTTTGCAGGCATATATTTAGCAAAGAACGTTCCAGCTGATAATAACATTGGACCAGTTGTTGAAGACGAGGAACTATTTGCTTCAGAATTTGTCACTTGTGTAGGTCAGGTTATAGGAGTGGTAGTTGCTGATAAACATGAGAATGCAAAACTAGCAGTGAGGAAGGTTCATGTTGAGTATGAAGAGCTACCAACCATCTTATCAATACAGGATGCCATCAATGCCAAAAGTTTCCATCCTAATACGGAGAGGTGTTTTAGGAAAGGGGATGTGGATATTTGCTTTCAATCAAGTCAATGTGACCATGTAATAGAGGGGGAAGTTCGAGTTGGCGGACAGGAACACTTCTATTTGGAGCCAAATAGCAGTGTGGTATGGACAGTGGATGGTGGCAATGAAGTTCATATGATCTCATCCACTCAGGCCCCTCAGAAGCACCAGAAATACATTTCTCATGTTCTTGGTCTTCCAATGTCGAAAGTCGTTTGTAAAACTAAGCGCATTGGTGGTGGTTTTGGCGGCAAGGAGACTAGGTCAGCTTTTATTGCTGCTGCAGCCGCTGTTCCAGCATATCTGTTAAATCGTCCAGTTAAAATTACATTAGGCCGTGATACAGATATGATGATAACTGGACAACGCCATAGCTTTCTCGGAAAGTACAAGGTTGGATTTACAAATGAGGGCAAGGTGTTGGCACTGGATCTTGAAATCTACAATAATGCTGGAAATTCGCTAGATTTGTCTCTTCCCGTACTTGAACGTGCTATGTTTCACTCGGATAACGTCTATGAGATTCCAAATGTGAGGATTGTAGGAAGGGTTTGCTTCACTAACATTCCAAGTAACACTGCTTTTCGAGGATTTGGTGGTCCTCAAGGTATGATTATTGCGGAAAATTGGATTCAGAGGGTTGCTGCAGAACTCAAAAAAAGCCCAGAAGAGATAAAAGAAATTAATTTTCAAGGTGAAGGATCAATATTGCATTATGGTCAACAACTTAAACACTGCACATTGGGTCCGCTCTGGAATCAACTGAAGTCATCTTGTGAATTTTCAAAGGCTCGCTATGAAGTTGACCAATTTAATATTCAAAATCGGTGGCGGAAGCGTGGTATTGCCATGGTTCCAACAAAATTTGGCATAGCCTTTACGTTAAAGCTTATGAACCAGGCAGGTGCTCTTGTTCATGTCTACACAGACGGAACTGTTTTAGTCACACATGGAGGTGTAGAGATGGGGCAAGGTTTACATACAAAAGTTGCTCAGGTCGCTGCTTCCGCCTTCAATATCCCTCTCAGTTCTGTCTTTATTTCGGAGACAAGTACTGACAAGGTTCCTAATGCATCACCAACAGCAGCTTCTGCGAGTTCTGACATGTATGGAGCTGCAGTTCTAGATGCATGTGAGAATATTAAGGCACGTATGAAGCCTATTGCTTCACAGCAAAATTTCAGCTCTTTTGCCGAGCTGGCAAGTGCATGCTATGTGGAACGGATAGATCTTTCTGCTCATGGGTTTTACATTACACCGGAAATTGACTTTGATTGGACAACTGGTAAAGGGAACCCATTCAACTACTTCACATATGGGGCTGCTTTTGCTGAGGTTGAAATTGATACATTGACTGGAGATTTTCACACCAGAGCGGCTAACATATTCTTGGATCTTGGTTACTCTCTCAACCCCGCAATTGATGTTGGGCAGATTGAAGGAGCATTTGTACAAGGTTTGGGATGGGTAGCTCTAGAAGAACTAAAGTGGGGCGATCCTGCTCATAAATGGATCTCACCCGGTTGCCTCTACACATCTGGGCCGGGAAGTTACAAGATTCCTTCCATTAATGATGTCCCCTTCAAATTCAGCATTTCACTTCTGAAGGGTCATCCAAATGTGAAGGCCATCCACTCATCTAAAGCTGTTGGTGAGCCACCATTTTTCTTGGCATCAGCCGTCTTCTTCGCCATCAAAGACGCCATAATAGCTGCAAGAGCAGAAGTAGGGTCTAATGAGTGGTTTCCACTTGATAATCCCGCGACACCTGAACGGATACGGATGGCCTGCTTAGACGAAATGATTGAACCATTTGTAAGCACAGATTTCCGCGCCAAGCTTAGCGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0000302 GO:0001101 GO:0003674 GO:0003824 GO:0004854 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006139 GO:0006144 GO:0006145 GO:0006725 GO:0006801 GO:0006807 GO:0006950 GO:0006979 GO:0008150 GO:0008152 GO:0009056 GO:0009112 GO:0009115 GO:0009414 GO:0009415 GO:0009628 GO:0009987 GO:0010035 GO:0016491 GO:0016725 GO:0016726 GO:0016903 GO:0019439 GO:0034641 GO:0036094 GO:0042221 GO:0042554 GO:0043167 GO:0043168 GO:0043546 GO:0044237 GO:0044238 GO:0044248 GO:0044270 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046110 GO:0046113 GO:0046483 GO:0046700 GO:0048037 GO:0050660 GO:0050662 GO:0050896 GO:0051536 GO:0051537 GO:0051540 GO:0055086 GO:0055114 GO:0071704 GO:0072521 GO:0072523 GO:0072593 GO:0097159 GO:1901265 GO:1901360 GO:1901361 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00230 ko00232 ko00983 ko01100 ko01110 ko01120 ko04146 map00230 map00232 map00983 map01100 map01110 map01120 map04146

Pfam Domains

Protein Families

Ald_Xan_dh_C Ald_Xan_dh_C2 CO_deh_flav_C FAD_binding_5 Fer2 Fer2_2

Protein Analysis

1369

Amino Acids

150.22

Weight (kDa)

6.58

Isoelectric Point (pI)

41.25

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Fer2	PF00111	27 - 89	2.5e-08	2Fe-2S iron-sulfur cluster binding domain
Fer2_2	PF01799	101 - 175	2.4e-32	[2Fe-2S] binding domain
FAD_binding_5	PF00941	270 - 447	2.2e-48	FAD binding domain in molybdopterin dehydrogenase
CO_deh_flav_C	PF03450	456 - 560	9.7e-31	CO dehydrogenase flavoprotein C-terminal domain
Ald_Xan_dh_C	PF01315	619 - 726	7.6e-29	Aldehyde oxidase and xanthine dehydrogenase, a/b hammerhead domain
MoCoBD_1	PF02738	744 - 982	6.6e-89	Molybdopterin cofactor-binding domain
MoCoBD_2	PF20256	1007 - 1279	1.1e-90	Molybdopterin cofactor-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0005756)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G34890 AT4G34900 AT4G34900 AT4G34900
fragaria_vesca	FvH4_3g29000 FvH4_3g29000 FvH4_3g29000 FvH4_3g29000
malus_domestica	MD03G1177200.v1.1 MD11G1161000.v1.1 MD11G1197900.v1.1
prunus_persica	Prupe.4G245700_v2.0.a1 Prupe.4G245700_v2.0.a1
pyrus_communis	pycom11g13410 pycom11g17110 pycom11g17120
rosa_chinensis	RchiOBHm_Chr5g0051661
rosa_laevigata	RLG00000034866
rosa_multiflora	Rmu_sc0000068.1_g000026 Rmu_sc0014882.1_g000001 Rmu_sc0018333.1_g000002
rosa_roxburghii	Rroxscaffold_1G00027170
rosa_rugosa	Rorug05G0277900
rosa_samantha	Rh5AG349700 Rh5BG361400 Rh5CG385900 Rh5DG376200
rosa_wichuraiana	Rw5G033070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	3149
Acc36I	ACCTGC	1 cut(s)	3149
AccB1I	GGYRCC	1 cut(s)	1565
AccBSI	CCGCTC	2 cut(s)	3007, 3632
AccI	GTMKAC	2 cut(s)	1945, 3177
AccII	CGCG	2 cut(s)	4019, 4089
AclI	AACGTT	1 cut(s)	2013
AclWI	GGATC	8 cut(s)	13, 1305, 1783, 2694, 2965, 3657, 3746, 3776
AcoI	YGGCCR	2 cut(s)	1302, 1760
AcuI	CTGAAG	5 cut(s)	248, 1832, 2052, 3041, 3881
AcyI	GRCGYC	1 cut(s)	3957
AdeI	CACNNNGTG	1 cut(s)	380
AfiI	CCNNNNNNNGG	5 cut(s)	328, 426, 611, 1294, 1772
AflIII	ACRYGT	1 cut(s)	3360
AgeI	ACCGGT	1 cut(s)	794
AjnI	CCWGG	6 cut(s)	30, 394, 1548, 1612, 1981, 3153
AjuI	GAANNNNNNNTTGG	6 cut(s)	2298, 2330, 3057, 3089, 3328, 3360
AloI	GAACNNNNNNTCC	2 cut(s)	3360, 3392
Alw21I	GWGCWC	1 cut(s)	3166
Alw26I	GTCTC	5 cut(s)	703, 1824, 2507, 2733, 3296
AlwI	GGATC	8 cut(s)	13, 1305, 1783, 2694, 2965, 3657, 3746, 3776
AlwNI	CAGNNNCTG	2 cut(s)	2546, 3347
Ama87I	CYCGRG	2 cut(s)	125, 1823
AseI	ATTAAT	3 cut(s)	561, 2940, 3825
AsiGI	ACCGGT	1 cut(s)	794
Asp700I	GAANNNNTTC	2 cut(s)	236, 3554
AspLEI	GCGC	2 cut(s)	2487, 4091
AspS9I	GGNCC	8 cut(s)	28, 109, 1347, 2036, 2410, 2855, 3002, 3797
AsuC2I	CCSGG	2 cut(s)	3777, 3801
AvaI	CYCGRG	2 cut(s)	125, 1823
AvaII	GGWCC	5 cut(s)	28, 1347, 2036, 2855, 3002
BaeGI	GKGCMC	1 cut(s)	605
BaeI	ACNNNNGTAYC	2 cut(s)	942, 975
BalI	TGGCCA	1 cut(s)	1304
BanI	GGYRCC	1 cut(s)	1565
BarI	GAAGNNNNNNTAC	2 cut(s)	845, 877
BbsI	GAAGAC	3 cut(s)	2055, 2444, 3931
Bbv12I	GWGCWC	1 cut(s)	3166
BbvCI	CCTCAGC	1 cut(s)	3588
BceAI	ACGGC	2 cut(s)	2577, 3920
BcgI	CGANNNNNNTGC	4 cut(s)	681, 715, 1382, 1416
BciT130I	CCWGG	6 cut(s)	32, 396, 1550, 1614, 1983, 3155
BciVI	GTATCC	1 cut(s)	4026
BcnI	CCSGG	2 cut(s)	3777, 3801
BcoDI	GTCTC	5 cut(s)	703, 1824, 2507, 2733, 3296
BfaI	CTAG	9 cut(s)	314, 845, 1212, 1344, 2138, 2517, 2720, 3380, 3731
BfmI	CTRYAG	4 cut(s)	279, 2538, 2901, 3372
BfuAI	ACCTGC	1 cut(s)	3149
BfuI	GTATCC	1 cut(s)	4026
BglI	GCCNNNNNGGC	1 cut(s)	3455
BglII	AGATCT	1 cut(s)	3484
BlpI	GCTNAGC	1 cut(s)	4097
BmcAI	AGTACT	1 cut(s)	3310
Bme1390I	CCNGG	8 cut(s)	32, 396, 1550, 1614, 1983, 3155, 3777, 3801
Bme18I	GGWCC	5 cut(s)	28, 1347, 2036, 2855, 3002
BmeT110I	CYCGRG	2 cut(s)	125, 1823
BmgT120I	GGNCC	8 cut(s)	28, 109, 1347, 2036, 2410, 2855, 3002, 3797
BmrFI	CCNGG	8 cut(s)	32, 396, 1550, 1614, 1983, 3155, 3777, 3801
BpiI	GAAGAC	3 cut(s)	2055, 2444, 3931
BpmI	CTGGAG	2 cut(s)	1986, 3633
Bpu10I	CCTNAGC	3 cut(s)	756, 3249, 3588
Bpu1102I	GCTNAGC	1 cut(s)	4097
BpuEI	CTTGAG	4 cut(s)	1454, 1583, 1764, 2844
BpuMI	CCSGG	2 cut(s)	3777, 3801
Bsa29I	ATCGAT	1 cut(s)	1956
BsaAI	YACGTR	1 cut(s)	3407
BsaHI	GRCGYC	1 cut(s)	3957
BsaI	GGTCTC	1 cut(s)	1824
BsaJI	CCNNGG	5 cut(s)	394, 1337, 1824, 1982, 3105
BsaWI	WCCGGW	2 cut(s)	794, 3514
BsaXI	ACNNNNNCTCC	8 cut(s)	2094, 2124, 2504, 2534, 3360, 3390, 3690, 3720
Bsc4I	CCNNNNNNNGG	5 cut(s)	328, 426, 611, 1294, 1772
Bse118I	RCCGGY	2 cut(s)	794, 1758
Bse3DI	GCAATG	4 cut(s)	1470, 1709, 1886, 2386
BseBI	CCWGG	6 cut(s)	32, 396, 1550, 1614, 1983, 3155
BseCI	ATCGAT	1 cut(s)	1956
BseDI	CCNNGG	5 cut(s)	394, 1337, 1824, 1982, 3105
BseLI	CCNNNNNNNGG	5 cut(s)	328, 426, 611, 1294, 1772
BseMI	GCAATG	4 cut(s)	1470, 1709, 1886, 2386
BseRI	GAGGAG	2 cut(s)	38, 467
BseSI	GKGCMC	1 cut(s)	605
BseYI	CCCAGC	3 cut(s)	1121, 1415, 1449
BsgI	GTGCAG	2 cut(s)	1086, 2977
Bsh1236I	CGCG	2 cut(s)	4019, 4089
Bsh1285I	CGRYCG	1 cut(s)	824
BshNI	GGYRCC	1 cut(s)	1565
BshTI	ACCGGT	1 cut(s)	794
BshVI	ATCGAT	1 cut(s)	1956
BsiEI	CGRYCG	1 cut(s)	824
BsiHKAI	GWGCWC	1 cut(s)	3166
BsiHKCI	CYCGRG	2 cut(s)	125, 1823
BsiSI	CCGG	5 cut(s)	795, 1759, 3515, 3777, 3800
BslFI	GGGAC	4 cut(s)	169, 1403, 1819, 3818
BslI	CCNNNNNNNGG	5 cut(s)	328, 426, 611, 1294, 1772
BsmAI	GTCTC	5 cut(s)	703, 1824, 2507, 2733, 3296
BsmFI	GGGAC	4 cut(s)	169, 1403, 1819, 3818
BsmI	GAATGC	2 cut(s)	1417, 2134
Bso31I	GGTCTC	1 cut(s)	1824
BsoBI	CYCGRG	2 cut(s)	125, 1823
Bsp1286I	GDGCHC	2 cut(s)	605, 3166
Bsp1407I	TGTACA	3 cut(s)	1166, 1744, 3706
Bsp1720I	GCTNAGC	1 cut(s)	4097
Bsp19I	CCATGG	1 cut(s)	3105
BspDI	ATCGAT	1 cut(s)	1956
BspFNI	CGCG	2 cut(s)	4019, 4089
BspHI	TCATGA	2 cut(s)	661, 1072
BspMAI	CTGCAG	3 cut(s)	2542, 2905, 3376
BspMI	ACCTGC	1 cut(s)	3149
BspPI	GGATC	8 cut(s)	13, 1305, 1783, 2694, 2965, 3657, 3746, 3776
BspQI	GCTCTTC	1 cut(s)	2163
BspT107I	GGYRCC	1 cut(s)	1565
BspTNI	GGTCTC	1 cut(s)	1824
BsrBI	CCGCTC	2 cut(s)	3007, 3632
BsrDI	GCAATG	4 cut(s)	1470, 1709, 1886, 2386
BsrFI	RCCGGY	2 cut(s)	794, 1758
BsrGI	TGTACA	3 cut(s)	1166, 1744, 3706
BssAI	RCCGGY	2 cut(s)	794, 1758
BssECI	CCNNGG	5 cut(s)	394, 1337, 1824, 1982, 3105
BssNAI	GTATAC	1 cut(s)	1946
BssNI	GRCGYC	1 cut(s)	3957
BssT1I	CCWWGG	2 cut(s)	1337, 3105
Bst1107I	GTATAC	1 cut(s)	1946
Bst2UI	CCWGG	6 cut(s)	32, 396, 1550, 1614, 1983, 3155
Bst4CI	ACNGT	9 cut(s)	69, 199, 304, 707, 825, 982, 1051, 2368, 3193
Bst6I	CTCTTC	5 cut(s)	15, 1532, 2163, 2736, 2919
BstACI	GRCGYC	1 cut(s)	3957
BstAPI	GCANNNNNTGC	3 cut(s)	334, 1472, 3347
BstAUI	TGTACA	3 cut(s)	1166, 1744, 3706
BstBAI	YACGTR	1 cut(s)	3407
BstC8I	GCNNGC	9 cut(s)	193, 259, 335, 1377, 1993, 3049, 3456, 3466, 4045
BstDSI	CCRYGG	1 cut(s)	3105
BstEII	GGTNACC	1 cut(s)	1283
BstENI	CCTNNNNNAGG	2 cut(s)	424, 1770
BstFNI	CGCG	2 cut(s)	4019, 4089
BstHHI	GCGC	2 cut(s)	2487, 4091
BstMAI	GTCTC	5 cut(s)	703, 1824, 2507, 2733, 3296
BstMCI	CGRYCG	1 cut(s)	824
BstNI	CCWGG	6 cut(s)	32, 396, 1550, 1614, 1983, 3155
BstNSI	RCATGY	5 cut(s)	261, 296, 3364, 3390, 3468
BstPI	GGTNACC	1 cut(s)	1283
BstSCI	CCNGG	8 cut(s)	30, 394, 1548, 1612, 1981, 3153, 3775, 3799
BstSFI	CTRYAG	4 cut(s)	279, 2538, 2901, 3372
BstSLI	GKGCMC	1 cut(s)	605
BstUI	CGCG	2 cut(s)	4019, 4089
BstV2I	GAAGAC	3 cut(s)	2055, 2444, 3931
BstX2I	RGATCY	6 cut(s)	1297, 1775, 2686, 3484, 3649, 3768
BstYI	RGATCY	6 cut(s)	1297, 1775, 2686, 3484, 3649, 3768
BstZ17I	GTATAC	1 cut(s)	1946
Bsu15I	ATCGAT	1 cut(s)	1956
BsuI	GTATCC	1 cut(s)	4026
BsuTUI	ATCGAT	1 cut(s)	1956
BtgI	CCRYGG	1 cut(s)	3105
BtsI	GCAGTG	4 cut(s)	2148, 2359, 2832, 2989
BveI	ACCTGC	1 cut(s)	3149
Cac8I	GCNNGC	9 cut(s)	193, 259, 335, 1377, 1993, 3049, 3456, 3466, 4045
CaiI	CAGNNNCTG	2 cut(s)	2546, 3347
CciI	TCATGA	2 cut(s)	661, 1072
CfoI	GCGC	2 cut(s)	2487, 4091
Cfr10I	RCCGGY	2 cut(s)	794, 1758
Cfr13I	GGNCC	8 cut(s)	28, 109, 1347, 2036, 2410, 2855, 3002, 3797
ClaI	ATCGAT	1 cut(s)	1956
CseI	GACGC	1 cut(s)	3965
CspAI	ACCGGT	1 cut(s)	794
CspCI	CAANNNNNGTGG	2 cut(s)	3065, 3100
DraIII	CACNNNGTG	1 cut(s)	380
EaeI	YGGCCR	2 cut(s)	1302, 1760
Eam1104I	CTCTTC	5 cut(s)	15, 1532, 2163, 2736, 2919
EarI	CTCTTC	5 cut(s)	15, 1532, 2163, 2736, 2919
EciI	GGCGGA	4 cut(s)	1880, 2334, 3103, 3253
Eco130I	CCWWGG	2 cut(s)	1337, 3105
Eco147I	AGGCCT	1 cut(s)	1094
Eco31I	GGTCTC	1 cut(s)	1824
Eco47I	GGWCC	5 cut(s)	28, 1347, 2036, 2855, 3002
Eco57I	CTGAAG	5 cut(s)	248, 1832, 2052, 3041, 3881
Eco88I	CYCGRG	2 cut(s)	125, 1823
Eco91I	GGTNACC	1 cut(s)	1283
EcoNI	CCTNNNNNAGG	2 cut(s)	424, 1770
EcoO109I	RGGNCCY	3 cut(s)	28, 1347, 2410
EcoO65I	GGTNACC	1 cut(s)	1283
EcoRII	CCWGG	6 cut(s)	30, 394, 1548, 1612, 1981, 3153
EcoT14I	CCWWGG	2 cut(s)	1337, 3105
EcoT22I	ATGCAT	5 cut(s)	294, 1468, 1709, 3331, 3388
ErhI	CCWWGG	2 cut(s)	1337, 3105
FalI	AAGNNNNNCTT	4 cut(s)	317, 349, 456, 488
FaqI	GGGAC	4 cut(s)	169, 1403, 1819, 3818
FauI	CCCGC	2 cut(s)	3679, 4024
FauNDI	CATATG	2 cut(s)	2391, 3571
FblI	GTMKAC	2 cut(s)	1945, 3177
FspBI	CTAG	9 cut(s)	314, 845, 1212, 1344, 2138, 2517, 2720, 3380, 3731
GlaI	GCGC	2 cut(s)	2486, 4090
GsaI	CCCAGC	3 cut(s)	1125, 1419, 1453
GsuI	CTGGAG	2 cut(s)	1986, 3633
HapII	CCGG	5 cut(s)	795, 1759, 3515, 3777, 3800
HgaI	GACGC	1 cut(s)	3965
HhaI	GCGC	2 cut(s)	2487, 4091
Hin1I	GRCGYC	1 cut(s)	3957
Hin6I	GCGC	2 cut(s)	2485, 4089
HinP1I	GCGC	2 cut(s)	2485, 4089
HincII	GTYRAC	3 cut(s)	253, 2978, 3061
HindII	GTYRAC	3 cut(s)	253, 2978, 3061
HindIII	AAGCTT	3 cut(s)	161, 3143, 4094
HinfI	GANTC	8 cut(s)	50, 350, 1420, 1961, 2779, 2887, 3013, 3814
HpaII	CCGG	5 cut(s)	795, 1759, 3515, 3777, 3800
Hpy166II	GTNNAC	9 cut(s)	253, 374, 1721, 1904, 1946, 2978, 3061, 3178, 3233
Hpy8I	GTNNAC	9 cut(s)	253, 374, 1721, 1904, 1946, 2978, 3061, 3178, 3233
HpyCH4III	ACNGT	9 cut(s)	69, 199, 304, 707, 825, 982, 1051, 2368, 3193
HpyCH4IV	ACGT	8 cut(s)	1063, 1801, 1948, 2013, 2746, 2769, 3137, 3406
HpySE526I	ACGT	8 cut(s)	1063, 1801, 1948, 2013, 2746, 2769, 3137, 3406
Hsp92I	GRCGYC	1 cut(s)	3957
HspAI	GCGC	2 cut(s)	2485, 4089
LguI	GCTCTTC	1 cut(s)	2163
LmnI	GCTCC	9 cut(s)	318, 454, 584, 1495, 1615, 1967, 2341, 3368, 3698
MaeI	CTAG	9 cut(s)	314, 845, 1212, 1344, 2138, 2517, 2720, 3380, 3731
MaeII	ACGT	8 cut(s)	1063, 1801, 1948, 2013, 2746, 2769, 3137, 3406
MbiI	CCGCTC	2 cut(s)	3007, 3632
MfeI	CAATTG	3 cut(s)	510, 1404, 3675
MflI	RGATCY	6 cut(s)	1297, 1775, 2686, 3484, 3649, 3768
MhlI	GDGCHC	2 cut(s)	605, 3166
MlsI	TGGCCA	1 cut(s)	1304
MluNI	TGGCCA	1 cut(s)	1304
MlyI	GAGTC	1 cut(s)	44
MmeI	TCCRAC	4 cut(s)	844, 1523, 2632, 3137
Mox20I	TGGCCA	1 cut(s)	1304
Mph1103I	ATGCAT	5 cut(s)	294, 1468, 1709, 3331, 3388
MroXI	GAANNNNTTC	2 cut(s)	236, 3554
MscI	TGGCCA	1 cut(s)	1304
MslI	CAYNNNNRTG	5 cut(s)	200, 666, 2127, 3210, 3873
Msp20I	TGGCCA	1 cut(s)	1304
MspA1I	CMGCKG	3 cut(s)	1811, 2021, 2546
MspI	CCGG	5 cut(s)	795, 1759, 3515, 3777, 3800
MspR9I	CCNGG	8 cut(s)	32, 396, 1550, 1614, 1983, 3155, 3777, 3801
MunI	CAATTG	3 cut(s)	510, 1404, 3675
Mva1269I	GAATGC	2 cut(s)	1417, 2134
MvaI	CCWGG	6 cut(s)	32, 396, 1550, 1614, 1983, 3155
MvnI	CGCG	2 cut(s)	4019, 4089
NciI	CCSGG	2 cut(s)	3777, 3801
NcoI	CCATGG	1 cut(s)	3105
NdeI	CATATG	2 cut(s)	2391, 3571
NmeAIII	GCCGAG	1 cut(s)	3475
NmuCI	GTSAC	4 cut(s)	949, 2077, 2285, 3199
NsiI	ATGCAT	5 cut(s)	294, 1468, 1709, 3331, 3388
NspI	RCATGY	5 cut(s)	261, 296, 3364, 3390, 3468
PaeI	GCATGC	2 cut(s)	261, 3468
PaeR7I	CTCGAG	1 cut(s)	125
PagI	TCATGA	2 cut(s)	661, 1072
PaqCI	CACCTGC	1 cut(s)	3149
PceI	AGGCCT	1 cut(s)	1094
PciI	ACATGT	1 cut(s)	3360
PciSI	GCTCTTC	1 cut(s)	2163
PctI	GAATGC	2 cut(s)	1417, 2134
PdmI	GAANNNNTTC	2 cut(s)	236, 3554
PfeI	GAWTC	7 cut(s)	350, 1420, 1961, 2779, 2887, 3013, 3814
PflFI	GACNNNGTC	2 cut(s)	1345, 2518
PinAI	ACCGGT	1 cut(s)	794
PleI	GAGTC	1 cut(s)	44
PpsI	GAGTC	1 cut(s)	44
Ppu21I	YACGTR	1 cut(s)	3407
PpuMI	RGGWCCY	2 cut(s)	28, 1347
PscI	ACATGT	1 cut(s)	3360
PshBI	ATTAAT	3 cut(s)	561, 2940, 3825
Psp1406I	AACGTT	1 cut(s)	2013
Psp5II	RGGWCCY	2 cut(s)	28, 1347
Psp6I	CCWGG	6 cut(s)	30, 394, 1548, 1612, 1981, 3153
PspEI	GGTNACC	1 cut(s)	1283
PspFI	CCCAGC	3 cut(s)	1121, 1415, 1449
PspGI	CCWGG	6 cut(s)	30, 394, 1548, 1612, 1981, 3153
PspPI	GGNCC	8 cut(s)	28, 109, 1347, 2036, 2410, 2855, 3002, 3797
PspPPI	RGGWCCY	2 cut(s)	28, 1347
PstI	CTGCAG	3 cut(s)	2542, 2905, 3376
PstNI	CAGNNNCTG	2 cut(s)	2546, 3347
PsuI	RGATCY	6 cut(s)	1297, 1775, 2686, 3484, 3649, 3768
PsyI	GACNNNGTC	2 cut(s)	1345, 2518
PvuII	CAGCTG	2 cut(s)	1811, 2021
RseI	CAYNNNNRTG	5 cut(s)	200, 666, 2127, 3210, 3873
SapI	GCTCTTC	1 cut(s)	2163
Sau96I	GGNCC	8 cut(s)	28, 109, 1347, 2036, 2410, 2855, 3002, 3797
ScaI	AGTACT	1 cut(s)	3310
SchI	GAGTC	1 cut(s)	44
ScrFI	CCNGG	8 cut(s)	32, 396, 1550, 1614, 1983, 3155, 3777, 3801
SduI	GDGCHC	2 cut(s)	605, 3166
SfcI	CTRYAG	4 cut(s)	279, 2538, 2901, 3372
Sfr274I	CTCGAG	1 cut(s)	125
SinI	GGWCC	5 cut(s)	28, 1347, 2036, 2855, 3002
SlaI	CTCGAG	1 cut(s)	125
SmiMI	CAYNNNNRTG	5 cut(s)	200, 666, 2127, 3210, 3873
SmlI	CTYRAG	5 cut(s)	125, 1433, 1598, 1779, 2859
SmoI	CTYRAG	5 cut(s)	125, 1433, 1598, 1779, 2859
SphI	GCATGC	2 cut(s)	261, 3468
SseBI	AGGCCT	1 cut(s)	1094
SspI	AATATT	5 cut(s)	925, 1249, 2964, 3073, 3397
SspMI	CTAG	9 cut(s)	314, 845, 1212, 1344, 2138, 2517, 2720, 3380, 3731
StuI	AGGCCT	1 cut(s)	1094
StyD4I	CCNGG	8 cut(s)	30, 394, 1548, 1612, 1981, 3153, 3775, 3799
StyI	CCWWGG	2 cut(s)	1337, 3105
TaaI	ACNGT	9 cut(s)	69, 199, 304, 707, 825, 982, 1051, 2368, 3193
TaiI	ACGT	8 cut(s)	1066, 1804, 1951, 2016, 2749, 2772, 3140, 3409
TaqI	TCGA	8 cut(s)	126, 608, 776, 1353, 1956, 2311, 2462, 2842
TatI	WGTACW	6 cut(s)	1166, 1437, 1744, 2643, 3308, 3706
TauI	GCSGC	3 cut(s)	2508, 2546, 3635
TfiI	GAWTC	7 cut(s)	350, 1420, 1961, 2779, 2887, 3013, 3814
TseFI	GTSAC	4 cut(s)	949, 2077, 2285, 3199
Tsp45I	GTSAC	4 cut(s)	949, 2077, 2285, 3199
TspGWI	ACGGA	5 cut(s)	2246, 3201, 3493, 4045, 4051
Tth111I	GACNNNGTC	2 cut(s)	1345, 2518
VpaK11BI	GGWCC	5 cut(s)	28, 1347, 2036, 2855, 3002
VspI	ATTAAT	3 cut(s)	561, 2940, 3825
XagI	CCTNNNNNAGG	2 cut(s)	424, 1770
XbaI	TCTAGA	3 cut(s)	1211, 3379, 3730
XceI	RCATGY	5 cut(s)	261, 296, 3364, 3390, 3468
XhoI	CTCGAG	1 cut(s)	125
XmiI	GTMKAC	2 cut(s)	1945, 3177
XmnI	GAANNNNTTC	2 cut(s)	236, 3554
XspI	CTAG	9 cut(s)	314, 845, 1212, 1344, 2138, 2517, 2720, 3380, 3731
ZrmI	AGTACT	1 cut(s)	3310
Zsp2I	ATGCAT	5 cut(s)	294, 1468, 1709, 3331, 3388

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.