Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5C

Orthogroup ID

OG0005756

Physical Location & Seq

Genomic Coordinates Forward (+)

50885559 .. 50899067

Gene Structure 13509 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5CG385900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3921 bp

ATGGGGTCGTTGTTGAAGAGCGAAGAGGAGGCCGGGGAGGAGGAGTCGAACGAGGCCATATTGTATGTTAATGGAATTCGTCGAGTCTTGCCTGATGGATTGGCTCACCTCACCCTCCTTGAGTACCTCAGAGATATAGGTCTCACGGGGACAAAGCTTGGTTGTGGTGAAGGTGGTTGTGGTGCGTGTACTGTAATGGTTTCGCATTATGACCAAAAACTGAAGAAATGCCTGCACTACGCCATTAATGCATGCTTGGCTCCACTGTATTCTTTAGAAGGGATGCACGTAATTACAGTGGAAGGACTTGGGAACCATAAACAAGGCCTGCACCCAATTCAAGAATCATTGGCACAATCTCATGGCTCACAATGTGGATTTTGCACCCCGGGTTTTATCATGTCCGTCTATGCATTATTAAGGTCAAGTCAGACCCCACCTAATGAGGAGCACATTGAAGAATGTTTAGCAGGAAACTTGTGTCGATGCACTGGTTACAGACCAATTGTTGATGCTTTTCGTGTCTTTGCCAAAACAGATGATAAACCGTACATTGACATATCATCTCTAAGTCTTGAAGGAGGTGAATTTGTGTGCCCTTCAACTGGTAAACCCTGTTCGTGTGGATTAAAAAGTGAGATATCAAGCAACAGTCACAAAACGGGTACTTGTGACGAGAGGTATGCACCTGTATCGTATAATGAAATTGATGGAAGCACATACACGGACAAGGAACTTATATTTCCTCCTGAGCTCATATTGAGAAAATCAACTTATTTACGTTTGAATGGATTCAGTGGGTTAAAGTGGTTCAGACCCTTGAGACTTAAGCAAGTACTAGAGTTAAAAGAAAAATACCCAGATGCAAAGTTATTAGTAGGTAATACTGAGGTAGGAATTGAAATGAGGTTGAAAAAAATTCAATATCGGGTTTTAATTTCTGTTACACATGTACCTGAACTTAGTATACTGAATCTGAAGGACGATGGACTTGAGATAGGTTCTGCGGTAAGACTTTCTGACCTACTTAAATTCTTAAGAAAGGTCATAACAGAACGTGCAGCTCATGAAACTTCTTCCTGTAAGGCTTTTGTTGAACAATTAAAATGGTTTGCTGGGATGCAAATAAGAAATGTTGCTTGTGTGGGTGGAAATATCTGTACAGCCAGTCCAATTTCAGATTTGAACCCCCTTTGGATGGCTGCCAGAGCAACTTTTCGAATTGTTGATTCCAAAGGGAACATTAGAACGACGCCGGCAGAAAAGTTTTTCCTCGGTTACCGTAAGGTGGATCTGGGGAGTGGTGAAATTCTACTTTCTGTATTTTTACCTTGGACTAGGCCGTTTGAGTATGTGAAAGAATATAAACAAGCTCACAGAAGGGATGATGATATTGCAATTGTGAATGCTGGAATTCGTGTTCATCTTGAGGAAAGAGGTGAAAACATTGTTGTTTCTGATGCATCTATTGTTTATGGGGGTGTAGCTCCACTCTCTCTTTCTGCAACAAGAACAAAAGACTTTCTCACTGGAAAGAGTTGGAACCAGGAGCTATTGCAGGGTGCTCTGAAAATCATCCAGAAGGATGTATTGCTCGGAGATAATGCTCCTGGTGGGATGGTGGAGTTTCGTAAGTCTTTGACACTTAGCTTTTTCTTCAAGTTTTTTCTGTGGGTTTCTCATCAGCTGGAAAGGGAAAAAGGCCTTAAGGGGAGTGTACCATTGTCTCATCTTTCTGCTATACAACCATTTGACAGGCCATCTGTTATAGGAACTCAAGACTATGAAATTACAAAACATGGGACAGCTGTTGGGTCTCCTGAGGTCCATCTATCATCGAGACTTCAGGTTTCAGGAGAGGCAGATTATGCTGATGATACACCATTGCCTCCCAATGGCTTGCATGCCGTGTTAATATTGAGCAAAAAACCTCATGCTCGTATACTTTCCATTGATGATTCAGGAGCAAAGTTATCGCCTGGGTTTGCAGGCATATTTTTTGCCAAGGATGTTCCAGCTGATAATAAAGTTGGACCCGTTGTTGTTGACGAGGAACTATTTGCTTCAGAGTTTGTCACTTGTGTAGGTCAGGTTATAGGAGTGGTAGTTGCAGATACACATGAAAAGGCAAAATTAGCAGCAATGAAAGTTCACGTTGAGTATGAAGAGCTCCCAGCCATCTTATCGATACAGGATGCCATCAATGCTAATAGTTTCCATCCTAATACTGAGAGGCGTTTTAGGAAAGGAGATGTGGATCTTTGCTTTCAATCATGTCAATGTGACAAGGTCATAGAGGGGGAAGTTCTAGTGGGAGGCCAGGAACACTTCTATTTGGAGCCACAGAGCAGTGTGATATGGACAGTGGATGGTGGCAATGAAGTTCATATGATCTCATCCACTCAAGCTCCTCAGAAGCACCAGAAGTATGTTTCTCATGTTCTTGGTCTTCCAATGTCAAAAGTAATTTGTAAAACGAAGCGTATTGGCGGCGGTTTTGGCGGCAAGGAGACAAGATCAGCTTTCATTGCTGCTGCAGCTTCTGTTCCTTCATATCTATTGAATCGACCAGTCAAGATTACATTAGACCGTGATACGGACATGATGATAACTGGGCAGCGCCATAGCTTTCTTGGAAAATACAAGGTGGGCTTTACAAATGAGGGAAAGGTGTTGGCATTGGATCTTCACATCTATAATAGTGCTGGAAATTCACTAGACTTGTCTCTTCCTGTGCTTGAGCGTGCTATGTTTCACTCGGATAATGTCTATGAGATTCCAAATGTGAGAATTGTGGGACGAGTTTGCTTCACTAATATACCCAGTAACACTGCTTTCCGAGGATTTGGTGGTCCTCAAGGGATGATTATTGCTGAAAATTGGATTCAAAGAATTGCCGTAGAACTGAATAAAAGCCCAGAAGAGATAAGAGAGATTAATTTTCAAGGTGAAGGATCAATATTGCATTATGGTCAGAAACTTCAACACTGTACACTGGCCCCGCTTTGGAACGAACTAAAACTATCTTGTGAATTTTCGAAGGCTCGCAGTGAAGTTTGCCAATTTAATACTCATAATCGGTGGCGGAAGCGTGGTGTAGCCATGATACCAACGAAGTTTGGCATATCATTTACATTAAAGCTCATGAACCAGGCAGGTGCTCTTGTTCATGTCTACACAGATGGAACTGTTCTAGTCACACATGGAGGAGTTGAGATGGGCCAAGGTTTGCATACAAAAGTTGCTCAGGTGGCTGCTTCTGCCTTCAATATTCCTCTCAGTTCAGTCTTCATATCAGAGACAAGTACCGACAAGGTTCCCAATGCTTCCCCAACAGCAGCGTCTGCAAGTTCTGACATGTATGGAGCTGCAGTCTTGGATGCATGTGAGCAAATCAAGGCGCGTATGGAACCTATTGCTTCACAGCACAATTTCAGCTCTTTTGCTGAGCTGGCAAGTGCATGCTATGTGGCCCGGATAGATCTTTCTGCCCATGGATTTTACATTGTACCTGAAATCGACTTTGATTGGACTACTGGTAAAGGCAACCCATTCAGGTACTTTACCTATGGAGCTGCTTTTGCTGAGGTTGAAATTGACACTTTGACTGGAGATTTCCACACTAGGGTGGCAAATATATTCTTGGATCTTGGATACTCTCTCAACCCAGCAATTGATGTTGGGCAGATCGAAGGAGCGTTTATACAAGGTTTGGGATGGGTTGCCCTAGAAGAACTAAAATGGGGAGATCCTGCTCATAAGTGGATTGCACCAGGTTCCCTCTACACTAGTGGGCCTGGAAGCTACAAAATTCCTTCCATAAATGACGTTCCCTTAAAATTCAATGTTTCACTTCTGAAGGTATGCTCATTATCCAGTAAGGGCATGTTATACGTTATATATTGTATCTGTGATGGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0000302 GO:0001101 GO:0003674 GO:0003824 GO:0004854 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006139 GO:0006144 GO:0006145 GO:0006725 GO:0006801 GO:0006807 GO:0006950 GO:0006979 GO:0008150 GO:0008152 GO:0009056 GO:0009112 GO:0009115 GO:0009414 GO:0009415 GO:0009628 GO:0009987 GO:0010035 GO:0016491 GO:0016725 GO:0016726 GO:0016903 GO:0019439 GO:0034641 GO:0036094 GO:0042221 GO:0042554 GO:0043167 GO:0043168 GO:0043546 GO:0044237 GO:0044238 GO:0044248 GO:0044270 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046110 GO:0046113 GO:0046483 GO:0046700 GO:0048037 GO:0050660 GO:0050662 GO:0050896 GO:0051536 GO:0051537 GO:0051540 GO:0055086 GO:0055114 GO:0071704 GO:0072521 GO:0072523 GO:0072593 GO:0097159 GO:1901265 GO:1901360 GO:1901361 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00230 ko00232 ko00983 ko01100 ko01110 ko01120 ko04146 map00230 map00232 map00983 map01100 map01110 map01120 map04146

Pfam Domains

Protein Families

Ald_Xan_dh_C Ald_Xan_dh_C2 CO_deh_flav_C FAD_binding_5 Fer2 Fer2_2

Protein Analysis

1306

Amino Acids

143.41

Weight (kDa)

6.38

Isoelectric Point (pI)

38.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Fer2	PF00111	36 - 88	1.1e-07	2Fe-2S iron-sulfur cluster binding domain
Fer2_2	PF01799	99 - 173	4.1e-32	[2Fe-2S] binding domain
FAD_binding_5	PF00941	268 - 445	8.2e-50	FAD binding domain in molybdopterin dehydrogenase
CO_deh_flav_C	PF03450	454 - 559	1.3e-28	CO dehydrogenase flavoprotein C-terminal domain
Ald_Xan_dh_C	PF01315	618 - 725	4.2e-28	Aldehyde oxidase and xanthine dehydrogenase, a/b hammerhead domain
MoCoBD_1	PF02738	741 - 981	1.8e-86	Molybdopterin cofactor-binding domain
MoCoBD_2	PF20256	1009 - 1278	1.1e-88	Molybdopterin cofactor-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0005756)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G34890 AT4G34900 AT4G34900 AT4G34900
fragaria_vesca	FvH4_3g29000 FvH4_3g29000 FvH4_3g29000 FvH4_3g29000
malus_domestica	MD03G1177200.v1.1 MD11G1161000.v1.1 MD11G1197900.v1.1
prunus_persica	Prupe.4G245700_v2.0.a1 Prupe.4G245700_v2.0.a1
pyrus_communis	pycom11g13410 pycom11g17110 pycom11g17120
rosa_chinensis	RchiOBHm_Chr5g0051661
rosa_laevigata	RLG00000034866
rosa_multiflora	Rmu_sc0000068.1_g000026 Rmu_sc0014882.1_g000001 Rmu_sc0018333.1_g000002
rosa_roxburghii	Rroxscaffold_1G00027170
rosa_rugosa	Rorug05G0277900
rosa_samantha	Rh5AG349700 Rh5BG361400 Rh5CG385900 Rh5DG376200
rosa_wichuraiana	Rw5G033070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	3146
Acc36I	ACCTGC	1 cut(s)	3146
AccI	GTMKAC	3 cut(s)	967, 1942, 3174
AccII	CGCG	1 cut(s)	3403
AciI	CCGC	6 cut(s)	1007, 2490, 2493, 2502, 3002, 3085
AclWI	GGATC	6 cut(s)	1299, 2265, 2691, 2962, 3654, 3743
AcuI	CTGAAG	5 cut(s)	242, 998, 1829, 2049, 3878
AcyI	GRCGYC	1 cut(s)	1253
AdeI	CACNNNGTG	1 cut(s)	374
AfiI	CCNNNNNNNGG	7 cut(s)	605, 1198, 1288, 1895, 2596, 3006, 3625
AflII	CTTAAG	3 cut(s)	827, 1036, 1706
AflIII	ACRYGT	2 cut(s)	949, 3357
AhlI	ACTAGT	1 cut(s)	3788
AjnI	CCWGG	7 cut(s)	1545, 1609, 1978, 2319, 3150, 3772, 3796
AleI	CACNNNNGTG	1 cut(s)	3626
AloI	GAACNNNNNNTCC	2 cut(s)	1404, 1436
Alw21I	GWGCWC	5 cut(s)	453, 756, 1567, 2172, 3163
Alw26I	GTCTC	8 cut(s)	146, 817, 1731, 1821, 1834, 2504, 2730, 3293
AlwI	GGATC	6 cut(s)	1299, 2265, 2691, 2962, 3654, 3743
AlwNI	CAGNNNCTG	2 cut(s)	2543, 3344
Ama87I	CYCGRG	1 cut(s)	388
AseI	ATTAAT	2 cut(s)	246, 2937
Asp700I	GAANNNNTTC	1 cut(s)	791
AspLEI	GCGC	2 cut(s)	2622, 3403
AspS9I	GGNCC	7 cut(s)	1825, 2033, 2852, 2998, 3220, 3472, 3794
AsuC2I	CCSGG	4 cut(s)	34, 389, 390, 3475
AsuHPI	GGTGA	7 cut(s)	98, 103, 179, 596, 1316, 1451, 2960
AsuII	TTCGAA	2 cut(s)	1219, 3038
AvaI	CYCGRG	1 cut(s)	388
AvaII	GGWCC	3 cut(s)	1825, 2033, 2852
AxyI	CCTNAGG	1 cut(s)	1821
BaeGI	GKGCMC	1 cut(s)	599
BaeI	ACNNNNGTAYC	2 cut(s)	936, 969
BanII	GRGCYC	2 cut(s)	756, 2172
BbsI	GAAGAC	2 cut(s)	2441, 3280
Bbv12I	GWGCWC	5 cut(s)	453, 756, 1567, 2172, 3163
BbvCI	CCTCAGC	1 cut(s)	3585
BceAI	ACGGC	3 cut(s)	1327, 1892, 2882
BcgI	CGANNNNNNTGC	6 cut(s)	665, 675, 699, 709, 1956, 1990
BciT130I	CCWGG	7 cut(s)	1547, 1611, 1980, 2321, 3152, 3774, 3798
BciVI	GTATCC	1 cut(s)	3647
BcnI	CCSGG	4 cut(s)	34, 389, 390, 3475
BcoDI	GTCTC	8 cut(s)	146, 817, 1731, 1821, 1834, 2504, 2730, 3293
BcuI	ACTAGT	1 cut(s)	3788
BfaI	CTAG	8 cut(s)	839, 1338, 2309, 2717, 3194, 3624, 3728, 3789
BfmI	CTRYAG	2 cut(s)	2535, 3369
BfoI	RGCGCY	1 cut(s)	2623
BfrI	CTTAAG	3 cut(s)	827, 1036, 1706
BfuAI	ACCTGC	1 cut(s)	3146
BfuI	GTATCC	1 cut(s)	3647
BglII	AGATCT	1 cut(s)	3481
BlpI	GCTNAGC	1 cut(s)	3447
BmcAI	AGTACT	1 cut(s)	837
Bme18I	GGWCC	3 cut(s)	1825, 2033, 2852
BmeT110I	CYCGRG	1 cut(s)	388
BmgT120I	GGNCC	7 cut(s)	1825, 2033, 2852, 2998, 3220, 3472, 3794
BmiI	GGNNCC	9 cut(s)	261, 314, 1544, 2035, 2340, 3000, 3318, 3411, 3778
BmrI	ACTGGG	2 cut(s)	2622, 2817
BmsI	GCATC	9 cut(s)	273, 476, 502, 853, 1110, 1450, 1472, 2185, 3370
BmuI	ACTGGG	2 cut(s)	2622, 2817
BpiI	GAAGAC	2 cut(s)	2441, 3280
BpmI	CTGGAG	1 cut(s)	3630
Bpu10I	CCTNAGC	3 cut(s)	750, 3246, 3585
Bpu1102I	GCTNAGC	1 cut(s)	3447
Bpu14I	TTCGAA	2 cut(s)	1219, 3038
BpuEI	CTTGAG	8 cut(s)	140, 841, 1013, 1448, 1761, 2388, 2759, 2841
BpuMI	CCSGG	4 cut(s)	34, 389, 390, 3475
Bsa29I	ATCGAT	1 cut(s)	2186
BsaAI	YACGTR	1 cut(s)	289
BsaBI	GATNNNNATC	1 cut(s)	2256
BsaHI	GRCGYC	1 cut(s)	1253
BsaI	GGTCTC	2 cut(s)	146, 1821
BsaXI	ACNNNNNCTCC	8 cut(s)	29, 59, 2091, 2121, 2392, 2422, 3357, 3387
Bsc4I	CCNNNNNNNGG	7 cut(s)	605, 1198, 1288, 1895, 2596, 3006, 3625
Bse118I	RCCGGY	1 cut(s)	1255
Bse21I	CCTNAGG	1 cut(s)	1821
Bse3DI	GCAATG	4 cut(s)	1883, 2148, 2383, 2526
Bse8I	GATNNNNATC	1 cut(s)	2256
BseBI	CCWGG	7 cut(s)	1547, 1611, 1980, 2321, 3152, 3774, 3798
BseCI	ATCGAT	1 cut(s)	2186
BseJI	GATNNNNATC	1 cut(s)	2256
BseLI	CCNNNNNNNGG	7 cut(s)	605, 1198, 1288, 1895, 2596, 3006, 3625
BseMI	GCAATG	4 cut(s)	1883, 2148, 2383, 2526
BseRI	GAGGAG	6 cut(s)	41, 53, 56, 461, 2400, 3222
BseSI	GKGCMC	1 cut(s)	599
BseYI	CCCAGC	3 cut(s)	1115, 2173, 3667
BsgI	GTGCAG	3 cut(s)	218, 314, 1080
Bsh1236I	CGCG	1 cut(s)	3403
BshVI	ATCGAT	1 cut(s)	2186
BsiHKAI	GWGCWC	5 cut(s)	453, 756, 1567, 2172, 3163
BsiHKCI	CYCGRG	1 cut(s)	388
BsiSI	CCGG	4 cut(s)	33, 389, 1256, 3475
BslFI	GGGAC	3 cut(s)	163, 1816, 2811
BslI	CCNNNNNNNGG	7 cut(s)	605, 1198, 1288, 1895, 2596, 3006, 3625
BsmAI	GTCTC	8 cut(s)	146, 817, 1731, 1821, 1834, 2504, 2730, 3293
BsmFI	GGGAC	3 cut(s)	163, 1816, 2811
BsmI	GAATGC	1 cut(s)	1411
Bso31I	GGTCTC	2 cut(s)	146, 1821
BsoBI	CYCGRG	1 cut(s)	388
Bsp119I	TTCGAA	2 cut(s)	1219, 3038
Bsp1286I	GDGCHC	6 cut(s)	453, 599, 756, 1567, 2172, 3163
Bsp1407I	TGTACA	2 cut(s)	1160, 2990
Bsp1720I	GCTNAGC	1 cut(s)	3447
Bsp19I	CCATGG	1 cut(s)	3493
BspACI	CCGC	6 cut(s)	1007, 2490, 2493, 2502, 3002, 3085
BspDI	ATCGAT	1 cut(s)	2186
BspFNI	CGCG	1 cut(s)	3403
BspHI	TCATGA	2 cut(s)	1066, 3144
BspLI	GGNNCC	9 cut(s)	261, 314, 1544, 2035, 2340, 3000, 3318, 3411, 3778
BspMAI	CTGCAG	2 cut(s)	2539, 3373
BspMI	ACCTGC	1 cut(s)	3146
BspPI	GGATC	6 cut(s)	1299, 2265, 2691, 2962, 3654, 3743
BspQI	GCTCTTC	2 cut(s)	11, 2160
BspT104I	TTCGAA	2 cut(s)	1219, 3038
BspTI	CTTAAG	3 cut(s)	827, 1036, 1706
BspTNI	GGTCTC	2 cut(s)	146, 1821
BsrDI	GCAATG	4 cut(s)	1883, 2148, 2383, 2526
BsrFI	RCCGGY	1 cut(s)	1255
BsrGI	TGTACA	2 cut(s)	1160, 2990
BssAI	RCCGGY	1 cut(s)	1255
BssNAI	GTATAC	2 cut(s)	968, 1943
BssNI	GRCGYC	1 cut(s)	1253
BssT1I	CCWWGG	4 cut(s)	1331, 2004, 3223, 3493
Bst1107I	GTATAC	2 cut(s)	968, 1943
Bst2UI	CCWGG	7 cut(s)	1547, 1611, 1980, 2321, 3152, 3774, 3798
Bst6I	CTCTTC	5 cut(s)	11, 18, 2160, 2733, 2916
BstACI	GRCGYC	1 cut(s)	1253
BstAFI	CTTAAG	3 cut(s)	827, 1036, 1706
BstAPI	GCANNNNNTGC	2 cut(s)	1868, 3344
BstAUI	TGTACA	2 cut(s)	1160, 2990
BstBAI	YACGTR	1 cut(s)	289
BstBI	TTCGAA	2 cut(s)	1219, 3038
BstDSI	CCRYGG	1 cut(s)	3493
BstEII	GGTNACC	1 cut(s)	1277
BstFNI	CGCG	1 cut(s)	3403
BstH2I	RGCGCY	1 cut(s)	2623
BstHHI	GCGC	2 cut(s)	2622, 3403
BstMAI	GTCTC	8 cut(s)	146, 817, 1731, 1821, 1834, 2504, 2730, 3293
BstNI	CCWGG	7 cut(s)	1547, 1611, 1980, 2321, 3152, 3774, 3798
BstNSI	RCATGY	7 cut(s)	255, 953, 1907, 3361, 3387, 3465, 3889
BstPI	GGTNACC	1 cut(s)	1277
BstSFI	CTRYAG	2 cut(s)	2535, 3369
BstSLI	GKGCMC	1 cut(s)	599
BstUI	CGCG	1 cut(s)	3403
BstV2I	GAAGAC	2 cut(s)	2441, 3280
BstX2I	RGATCY	6 cut(s)	1291, 2257, 2683, 3481, 3646, 3748
BstYI	RGATCY	6 cut(s)	1291, 2257, 2683, 3481, 3646, 3748
BstZ17I	GTATAC	2 cut(s)	968, 1943
Bsu15I	ATCGAT	1 cut(s)	2186
Bsu36I	CCTNAGG	1 cut(s)	1821
BsuI	GTATCC	1 cut(s)	3647
BsuTUI	ATCGAT	1 cut(s)	2186
BtgI	CCRYGG	1 cut(s)	3493
BtsI	GCAGTG	3 cut(s)	2356, 2829, 3055
BveI	ACCTGC	1 cut(s)	3146
CaiI	CAGNNNCTG	2 cut(s)	2543, 3344
CciI	TCATGA	2 cut(s)	1066, 3144
CfoI	GCGC	2 cut(s)	2622, 3403
Cfr10I	RCCGGY	1 cut(s)	1255
Cfr13I	GGNCC	7 cut(s)	1825, 2033, 2852, 2998, 3220, 3472, 3794
Cfr9I	CCCGGG	1 cut(s)	388
ClaI	ATCGAT	1 cut(s)	2186
CseI	GACGC	2 cut(s)	1261, 3330
CsiI	ACCWGGT	1 cut(s)	3772
DraIII	CACNNNGTG	1 cut(s)	374
Eam1104I	CTCTTC	5 cut(s)	11, 18, 2160, 2733, 2916
EarI	CTCTTC	5 cut(s)	11, 18, 2160, 2733, 2916
EciI	GGCGGA	1 cut(s)	3100
Ecl136II	GAGCTC	2 cut(s)	754, 2170
Eco130I	CCWWGG	4 cut(s)	1331, 2004, 3223, 3493
Eco147I	AGGCCT	2 cut(s)	327, 1704
Eco24I	GRGCYC	2 cut(s)	756, 2172
Eco31I	GGTCTC	2 cut(s)	146, 1821
Eco32I	GATATC	1 cut(s)	642
Eco47I	GGWCC	3 cut(s)	1825, 2033, 2852
Eco53kI	GAGCTC	2 cut(s)	754, 2170
Eco57I	CTGAAG	5 cut(s)	242, 998, 1829, 2049, 3878
Eco81I	CCTNAGG	1 cut(s)	1821
Eco88I	CYCGRG	1 cut(s)	388
Eco91I	GGTNACC	1 cut(s)	1277
EcoICRI	GAGCTC	2 cut(s)	754, 2170
EcoO65I	GGTNACC	1 cut(s)	1277
EcoRI	GAATTC	2 cut(s)	75, 1413
EcoRII	CCWGG	7 cut(s)	1545, 1609, 1978, 2319, 3150, 3772, 3796
EcoRV	GATATC	1 cut(s)	642
EcoT14I	CCWWGG	4 cut(s)	1331, 2004, 3223, 3493
EcoT22I	ATGCAT	4 cut(s)	253, 415, 1465, 3385
EcoT38I	GRGCYC	2 cut(s)	756, 2172
ErhI	CCWWGG	4 cut(s)	1331, 2004, 3223, 3493
FalI	AAGNNNNNCTT	4 cut(s)	2506, 2538, 2636, 2668
FaqI	GGGAC	3 cut(s)	163, 1816, 2811
FauI	CCCGC	1 cut(s)	3009
FauNDI	CATATG	1 cut(s)	2388
FblI	GTMKAC	3 cut(s)	967, 1942, 3174
FriOI	GRGCYC	2 cut(s)	756, 2172
FspBI	CTAG	8 cut(s)	839, 1338, 2309, 2717, 3194, 3624, 3728, 3789
GlaI	GCGC	2 cut(s)	2621, 3402
GsaI	CCCAGC	3 cut(s)	1119, 2177, 3671
GsuI	CTGGAG	1 cut(s)	3630
HaeII	RGCGCY	1 cut(s)	2623
HapII	CCGG	4 cut(s)	33, 389, 1256, 3475
HgaI	GACGC	2 cut(s)	1261, 3330
HhaI	GCGC	2 cut(s)	2622, 3403
Hin1I	GRCGYC	1 cut(s)	1253
Hin6I	GCGC	2 cut(s)	2620, 3401
HinP1I	GCGC	2 cut(s)	2620, 3401
HincII	GTYRAC	1 cut(s)	2047
HindII	GTYRAC	1 cut(s)	2047
HindIII	AAGCTT	1 cut(s)	155
HpaII	CCGG	4 cut(s)	33, 389, 1256, 3475
HphI	GGTGA	7 cut(s)	98, 103, 179, 596, 1316, 1451, 2960
Hpy166II	GTNNAC	9 cut(s)	189, 611, 968, 1718, 1943, 2047, 2152, 2993, 3175
Hpy8I	GTNNAC	9 cut(s)	189, 611, 968, 1718, 1943, 2047, 2152, 2993, 3175
Hpy99I	CGWCG	2 cut(s)	84, 1255
HpyCH4IV	ACGT	6 cut(s)	288, 781, 1057, 2154, 3828, 3894
HpySE526I	ACGT	6 cut(s)	288, 781, 1057, 2154, 3828, 3894
Hsp92I	GRCGYC	1 cut(s)	1253
HspAI	GCGC	2 cut(s)	2620, 3401
KroI	GCCGGC	1 cut(s)	1255
KroNI	GCCGGC	1 cut(s)	1257
LguI	GCTCTTC	2 cut(s)	11, 2160
LweI	GCATC	9 cut(s)	273, 476, 502, 853, 1110, 1450, 1472, 2185, 3370
MabI	ACCWGGT	1 cut(s)	3772
MaeI	CTAG	8 cut(s)	839, 1338, 2309, 2717, 3194, 3624, 3728, 3789
MaeII	ACGT	6 cut(s)	288, 781, 1057, 2154, 3828, 3894
MaeIII	GTNAC	9 cut(s)	494, 653, 671, 943, 1277, 2074, 2282, 2825, 3196
MfeI	CAATTG	3 cut(s)	504, 1398, 3672
MflI	RGATCY	6 cut(s)	1291, 2257, 2683, 3481, 3646, 3748
MhlI	GDGCHC	6 cut(s)	453, 599, 756, 1567, 2172, 3163
MlyI	GAGTC	2 cut(s)	53, 93
MmeI	TCCRAC	2 cut(s)	1520, 2011
Mph1103I	ATGCAT	4 cut(s)	253, 415, 1465, 3385
MroNI	GCCGGC	1 cut(s)	1255
MroXI	GAANNNNTTC	1 cut(s)	791
MslI	CAYNNNNRTG	2 cut(s)	2278, 3626
MspA1I	CMGCKG	3 cut(s)	1687, 1808, 2018
MspCI	CTTAAG	3 cut(s)	827, 1036, 1706
MspI	CCGG	4 cut(s)	33, 389, 1256, 3475
MunI	CAATTG	3 cut(s)	504, 1398, 3672
Mva1269I	GAATGC	1 cut(s)	1411
MvaI	CCWGG	7 cut(s)	1547, 1611, 1980, 2321, 3152, 3774, 3798
MvnI	CGCG	1 cut(s)	3403
NaeI	GCCGGC	1 cut(s)	1257
NciI	CCSGG	4 cut(s)	34, 389, 390, 3475
NcoI	CCATGG	1 cut(s)	3493
NdeI	CATATG	1 cut(s)	2388
NgoMIV	GCCGGC	1 cut(s)	1255
NlaIV	GGNNCC	9 cut(s)	261, 314, 1544, 2035, 2340, 3000, 3318, 3411, 3778
NmuCI	GTSAC	5 cut(s)	653, 671, 2074, 2282, 3196
NsiI	ATGCAT	4 cut(s)	253, 415, 1465, 3385
NspI	RCATGY	7 cut(s)	255, 953, 1907, 3361, 3387, 3465, 3889
NspV	TTCGAA	2 cut(s)	1219, 3038
OliI	CACNNNNGTG	1 cut(s)	3626
PaeI	GCATGC	3 cut(s)	255, 1907, 3465
PagI	TCATGA	2 cut(s)	1066, 3144
PaqCI	CACCTGC	1 cut(s)	3146
PceI	AGGCCT	2 cut(s)	327, 1704
PciI	ACATGT	2 cut(s)	949, 3357
PciSI	GCTCTTC	2 cut(s)	11, 2160
PctI	GAATGC	1 cut(s)	1411
PdiI	GCCGGC	1 cut(s)	1257
PdmI	GAANNNNTTC	1 cut(s)	791
PfeI	GAWTC	8 cut(s)	344, 792, 973, 1229, 1958, 2563, 2776, 2884
PflFI	GACNNNGTC	1 cut(s)	2288
PleI	GAGTC	2 cut(s)	52, 92
PpsI	GAGTC	2 cut(s)	52, 92
Ppu21I	YACGTR	1 cut(s)	289
PscI	ACATGT	2 cut(s)	949, 3357
PshBI	ATTAAT	2 cut(s)	246, 2937
Psp124BI	GAGCTC	2 cut(s)	756, 2172
Psp6I	CCWGG	7 cut(s)	1545, 1609, 1978, 2319, 3150, 3772, 3796
PspEI	GGTNACC	1 cut(s)	1277
PspFI	CCCAGC	3 cut(s)	1115, 2173, 3667
PspGI	CCWGG	7 cut(s)	1545, 1609, 1978, 2319, 3150, 3772, 3796
PspN4I	GGNNCC	9 cut(s)	261, 314, 1544, 2035, 2340, 3000, 3318, 3411, 3778
PspPI	GGNCC	7 cut(s)	1825, 2033, 2852, 2998, 3220, 3472, 3794
PsrI	GAACNNNNNNTAC	4 cut(s)	601, 633, 951, 983
PstI	CTGCAG	2 cut(s)	2539, 3373
PstNI	CAGNNNCTG	2 cut(s)	2543, 3344
PsuI	RGATCY	6 cut(s)	1291, 2257, 2683, 3481, 3646, 3748
PsyI	GACNNNGTC	1 cut(s)	2288
PvuII	CAGCTG	3 cut(s)	1687, 1808, 2018
RseI	CAYNNNNRTG	2 cut(s)	2278, 3626
SacI	GAGCTC	2 cut(s)	756, 2172
SapI	GCTCTTC	2 cut(s)	11, 2160
Sau96I	GGNCC	7 cut(s)	1825, 2033, 2852, 2998, 3220, 3472, 3794
ScaI	AGTACT	1 cut(s)	837
SchI	GAGTC	2 cut(s)	53, 93
SduI	GDGCHC	6 cut(s)	453, 599, 756, 1567, 2172, 3163
SexAI	ACCWGGT	1 cut(s)	3772
SfaNI	GCATC	9 cut(s)	273, 476, 502, 853, 1110, 1450, 1472, 2185, 3370
SfcI	CTRYAG	2 cut(s)	2535, 3369
SfuI	TTCGAA	2 cut(s)	1219, 3038
SinI	GGWCC	3 cut(s)	1825, 2033, 2852
SmaI	CCCGGG	1 cut(s)	390
SmiMI	CAYNNNNRTG	2 cut(s)	2278, 3626
SpeI	ACTAGT	1 cut(s)	3788
SphI	GCATGC	3 cut(s)	255, 1907, 3465
SseBI	AGGCCT	2 cut(s)	327, 1704
SsiI	CCGC	6 cut(s)	1007, 2490, 2493, 2502, 3002, 3085
SspI	AATATT	3 cut(s)	1917, 2961, 3271
SspMI	CTAG	8 cut(s)	839, 1338, 2309, 2717, 3194, 3624, 3728, 3789
SstI	GAGCTC	2 cut(s)	756, 2172
StuI	AGGCCT	2 cut(s)	327, 1704
StyI	CCWWGG	4 cut(s)	1331, 2004, 3223, 3493
TaiI	ACGT	6 cut(s)	291, 784, 1060, 2157, 3831, 3897
TatI	WGTACW	4 cut(s)	188, 835, 1160, 2990
TauI	GCSGC	2 cut(s)	2493, 2505
TfiI	GAWTC	8 cut(s)	344, 792, 973, 1229, 1958, 2563, 2776, 2884
TseFI	GTSAC	5 cut(s)	653, 671, 2074, 2282, 3196
Tsp45I	GTSAC	5 cut(s)	653, 671, 2074, 2282, 3196
TspGWI	ACGGA	3 cut(s)	394, 740, 2612
TspMI	CCCGGG	1 cut(s)	388
Tth111I	GACNNNGTC	1 cut(s)	2288
Vha464I	CTTAAG	3 cut(s)	827, 1036, 1706
VpaK11BI	GGWCC	3 cut(s)	1825, 2033, 2852
VspI	ATTAAT	2 cut(s)	246, 2937
XceI	RCATGY	7 cut(s)	255, 953, 1907, 3361, 3387, 3465, 3889
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2630
XmaI	CCCGGG	1 cut(s)	388
XmiI	GTMKAC	3 cut(s)	967, 1942, 3174
XmnI	GAANNNNTTC	1 cut(s)	791
XspI	CTAG	8 cut(s)	839, 1338, 2309, 2717, 3194, 3624, 3728, 3789
ZrmI	AGTACT	1 cut(s)	837
Zsp2I	ATGCAT	4 cut(s)	253, 415, 1465, 3385

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.