Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0005756

Physical Location & Seq

Genomic Coordinates Reverse (-)

34249468 .. 34262049

Gene Structure 12582 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00027170.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4098 bp

ATGGGGTCGTTGAAGAGCGAAGAGGAGGCCGGGGAGGAGTCGAACGAGGCCATATTGTATGTTAATGGAATTCGTCGAGTCTTGCCTGATGGATTGGCTCACCTCACCCTCCTTGAGTACCTCAGAGACATAGGTCTCACAGGGACAAAGCTTGGTTGTGGTGAAGGTGGTTGTGGTGCGTGTACTGTAATGGTTTCGCATTATGACCAAAAACTGAAGAAATGCCTGCACTACGCCGTTAATGCGTGCTTGGCTCCACTGTATTCTTTAGAAGGGATGCACGTAATTACAGTGGAAGGACTTGGGAACCATAAACAAGGCCTGCACCCAATTCAAGAATCATTGGCACAATCTCATGGCTCACAATGTGGATTTTGCACCCCGGGTTTTATCATGTCCGTCTATGCATTATTAAGGTCAAGTCAGACCCCACCTAATGAGGAACAGATTGAAGAATGTTTAGCAGGAAACTTGTGTCGATGCACTGGTTACAGACCAATTGTTGATGCTTTTCGTGTCTTTGCCAAAACAGATGATAAACCGTACATTGACATATCTTCTCTAAGCCTTGAAGGAGGTGAATTTGTGTGCCCTTCAACTGGTAAACCCTGTTCGTGTGGATTAAAAAGTGAGATATCAAGCAACCGTCACAAAACGGGTACTTGTGACGAGAGGTATGCACCTGTATCGTATAGTGAAATTGATGGAAGCACATACACGGACAAGGAACTTATATTTCCTCCTGAGCTCATATTGAGAAAATCAACTTATTTACATTTGAATGGATTCAGTGGGTTAAAGTGGTTCAGACCCTTGAGACTTAAGCAAGTACTAGAGTTAAAAGAAAAATACCCAGATGCAAAGTTATTAGTAGGTAATACTGAGGTAGGAATTGAAATGAGGTTGAAAAAAATTCAATATCGGGTTTTAATTTCTGTTACACATGTACCTGAACTTAGTATTCTGAATCTGAAGGATGATGGACTTGAGATAGGTTCTGCGGTAAGACTTTCTGAACTACTTAAATTCTTAAGAAAGGTCATAACAGAACGTGCAGCTCATGAAACTTCTTCCTGTAAGGCTTTTGTTGAACAATTAAAATGGTTTGCTGGGATGCAAATAAGAAATGTTGCTTGTGTGGGTGGAAATATCTGTACAGCCAGTCCAATTTCAGACTTGAACCCCCTTTGGATGGCTGCCAGAGCAACTTTTCGAATTGTTGATTCCAAAGGGAACATTAGAACGACGCCGGCAGAAAAGTTTTTCCTCGGTTACCGTAAGGTGGATCTGGGAAGTGGTGAAATTCTACTTTCTGTATTTTTACCTTGGACTAGGCCGTTTGAGTATGTGAAAGAATATAAACAAGCTCACAGAAGGGATGATGATATTGCAATTGTGAATGCTGGAATTCGTGTTCATCTTGAGGAAAGAGGTGAAAACATTGTTGTTTCTGATGCATCTATTGTTTATGGGGGTGTAGCTCCACTCTCTCTTTCTGCAACAAGAACAAAAGACTTTCTCACTGGAAAGAGTTGGAACCAGGAGCTATTGCAGGGTGCTCTGAAAATCATCCAGAAGGATGTATTGCTTGGAGATAATGCTCCTGGTGGGATGGTGGAGTTTCGTAAGTCATTGACACTTAGCTTCTTCTTCAAGTTTTTTCTGTGGGTTTCTCATCAGCTGGAAAGGGAAAAAGGCCTTAAGGGGAGTGTACCATTGTCTCATCTTTCTGCTATACAACCATTTGACAGGCCATCTGTTAGAGGAACTCAAGACTATGAAATTACAAAACATGGGACAGCTGTTGGGTCTCCTGAGGTTCATCTATCATCAAGACTTCAGGTTTCAGGAGAGGCAGAATATGCTGATGATACACCATTGCCTCCCAATGGCTTGCATGCCGCGTTAATATTGAGCAAAAAACCTCATGCTCGTATACTTTCAATTGATGATTCAGGAGCAAAGTTATCGCCTGGGTTTGCAGGCATATTTTTTGCCAAGGATGTTCCAGCTGATAATAAAGTTGGTCCCGTTGTTGTTGACGAGGAACTATTTGCTTCAGAGTTTGTCACTTGTGTAGGTCAGGTTATAGGAGTGGTAGTTGCAGATACACATGAAAAGGCAAAATTAGCAGCGATGAAAGTTCACGTTGAGTATGAAGAGCTCCCAGCCATCTTATCGATACAGGATGCCATCAATGCTAATAGTTTCCATCCTAATACTGAGAGGCGTTTTAGGAAAGGAGATGTGGATCTTTGCTTTCAATCATGTCAATGTGACAACGTCATAGAGGGGGAAGTTCTAGTGGGAGGCCAGGAACACTTCTATTTGGAGCCACAGAGCAGTGTGATATGGACAATGGATGGTGGCAATGAAGTTCATATGATCTCATCCACTCAAGCTCCTCAGAAGCACCAGAAGTATGTTTCTCATGTTCTTGGTCTTCCAATGTCAAAAGTAATTTGTAAAACGAAGCGTATTGGCGGCGGTTTTGGCGGCAAGGAGACAAGATCAGCTTTCATTGCTGCTGCAGCTTCTGTTCCTTCATATCTATTGAATCGACCAGTCAAAATTACATTAGACCGTGACACGGACATGATGATAACTGGGCAGCGCCATAGCTTTCTTGGAAAATACAAGGTGGGCTTTACAAATGAGGGAAAGGTGTTGGCATTGGATCTTCTCATCTATAATAGTGCTGGAAATTCACTAGACTTGTCTCTTCCTGTGCTTGAGCGTGCTATGTTTCACTCGGATAATGTCTATGAGATTCCAAATGTGAGAATTGTGGGACGAGTTTGCTTCACTAATATACCCAGTAACACTGCTTTCCGAGGATTTGGTGGTCCTCAAGGGATGATTATTGCTGAAAATTGGATTCAAAGAATTGCCGTAGAACTGAATAAAAGCCCAGAAGAGATAAGAGAGATTAATTTTCAAGGTGAAGGATCAATATTGCATTATGGTCAGAAACTTCAACACTGTACGCTGGCCCCGCTTTGGAACGAACTAAAACTATCTTGTGAATTTTCAAAGGCTCGCAGTGAAGTTTGCCGATTTAATACTCATAATCGGTGGCGGAAGCGTGGTGTAGCCATGATACCAACGAAGTTTGGCATATCATTTACATTAAAGCTCATGAACCAGGCAGGTGCTCTTGTTCATGTCTACACAGATGGAACTGTTCTAGTCACACATGGAGGAGTTGAGATGGGGCAAGGTTTGCATACAAAAGTTGCTCAGGTGGCTGCTTCTGCCTTCAATATTCCTCTCAGTTCAGTCTTCATATCAGAGACAAGTACCGACAAGGTTCCTAATGCTTCCCCAACAGCAGCGTCTGCAAGTTCTGACATGTATGGAGCTGCAGTCTTGGATGCATGTGAGCAAATCAAGGCACGTATGGAACCTATTGCTTCACAGCACAATTTCAGCTCTTTTGCTGAGCTGGCAAGTGCATGCTATGTGGCCCGGATAGATCTTTCTGCCCATGGATTTTACATTGTACCTGAAATCGACTTTGATTGGACTACTGGTAAAGGCAACCCATTCAGGTACTATACCTATGGGGCTGCTTTTGCTGAGGTTGAAATTGACACTTTGACTGGAGATTTCCACACTAGGGTGGCAAATATATTCTTGGATCTTGGATACTCTCTCAACCCAGCAATTGATGTTGGGCAGATCGAAGGAGCGTTTATACAAGGTTTGGGATGGGTTGCCCTAGAAGAACTAAAATGGGGAGATCCTGCTCATAAGTGGATTGCACCAGGTTCCCTCTACACTTGTGGGCCTGGAAGCTACAAAATTCCTTCCATAAATGACGTTCCCTTAAAATTCAATGTTTCACTTCTGAAGGGACATCCAAATGTAAAGGCCATCCACTCATCTAAAGCTGTTGGTGAGCCCCCATTTTTCTTGGCATCAGCTGTCTTTTTTGCTATCAAAGATGCCATTATAGCTGCAAGAGCTGAAGTAGGGTGTAATGAGTGGTTTCCACTTGATAATCCAGCAACACCAGAACGTATACGGATGGCTTGTTTGGATGAATTTACCGCAGCATTTGCTAGCTCAGATTTTCGCGCAAAGCTTAGTGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0000302 GO:0001101 GO:0003674 GO:0003824 GO:0004854 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006139 GO:0006144 GO:0006145 GO:0006725 GO:0006801 GO:0006807 GO:0006950 GO:0006979 GO:0008150 GO:0008152 GO:0009056 GO:0009112 GO:0009115 GO:0009414 GO:0009415 GO:0009628 GO:0009987 GO:0010035 GO:0016491 GO:0016725 GO:0016726 GO:0016903 GO:0019439 GO:0034641 GO:0036094 GO:0042221 GO:0042554 GO:0043167 GO:0043168 GO:0043546 GO:0044237 GO:0044238 GO:0044248 GO:0044270 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046110 GO:0046113 GO:0046483 GO:0046700 GO:0048037 GO:0050660 GO:0050662 GO:0050896 GO:0051536 GO:0051537 GO:0051540 GO:0055086 GO:0055114 GO:0071704 GO:0072521 GO:0072523 GO:0072593 GO:0097159 GO:1901265 GO:1901360 GO:1901361 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00230 ko00232 ko00983 ko01100 ko01110 ko01120 ko04146 map00230 map00232 map00983 map01100 map01110 map01120 map04146

Pfam Domains

Protein Families

Ald_Xan_dh_C Ald_Xan_dh_C2 CO_deh_flav_C FAD_binding_5 Fer2 Fer2_2

Protein Analysis

1365

Amino Acids

149.85

Weight (kDa)

6.45

Isoelectric Point (pI)

38.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Fer2	PF00111	34 - 86	1.1e-07	2Fe-2S iron-sulfur cluster binding domain
Fer2_2	PF01799	97 - 171	1.9e-32	[2Fe-2S] binding domain
FAD_binding_5	PF00941	266 - 443	7.6e-50	FAD binding domain in molybdopterin dehydrogenase
CO_deh_flav_C	PF03450	452 - 557	1.4e-28	CO dehydrogenase flavoprotein C-terminal domain
Ald_Xan_dh_C	PF01315	616 - 723	1.1e-28	Aldehyde oxidase and xanthine dehydrogenase, a/b hammerhead domain
MoCoBD_1	PF02738	739 - 979	1.3e-85	Molybdopterin cofactor-binding domain
MoCoBD_2	PF20256	1007 - 1276	3.1e-88	Molybdopterin cofactor-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0005756)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G34890 AT4G34900 AT4G34900 AT4G34900
fragaria_vesca	FvH4_3g29000 FvH4_3g29000 FvH4_3g29000 FvH4_3g29000
malus_domestica	MD03G1177200.v1.1 MD11G1161000.v1.1 MD11G1197900.v1.1
prunus_persica	Prupe.4G245700_v2.0.a1 Prupe.4G245700_v2.0.a1
pyrus_communis	pycom11g13410 pycom11g17110 pycom11g17120
rosa_chinensis	RchiOBHm_Chr5g0051661
rosa_laevigata	RLG00000034866
rosa_multiflora	Rmu_sc0000068.1_g000026 Rmu_sc0014882.1_g000001 Rmu_sc0018333.1_g000002
rosa_roxburghii	Rroxscaffold_1G00027170
rosa_rugosa	Rorug05G0277900
rosa_samantha	Rh5AG349700 Rh5BG361400 Rh5CG385900 Rh5DG376200
rosa_wichuraiana	Rw5G033070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	3140
Acc36I	ACCTGC	1 cut(s)	3140
AccI	GTMKAC	3 cut(s)	1936, 3168, 4024
AccII	CGCG	2 cut(s)	1904, 4080
AciI	CCGC	8 cut(s)	1001, 1902, 2484, 2487, 2496, 2996, 3079, 4053
AclWI	GGATC	6 cut(s)	1293, 2259, 2685, 2956, 3648, 3737
AcuI	CTGAAG	6 cut(s)	236, 992, 1823, 2043, 3872, 3990
AcyI	GRCGYC	1 cut(s)	1247
AdeI	CACNNNGTG	1 cut(s)	368
AfiI	CCNNNNNNNGG	7 cut(s)	599, 1192, 1282, 1889, 2590, 3000, 3619
AflII	CTTAAG	3 cut(s)	821, 1030, 1700
AflIII	ACRYGT	2 cut(s)	943, 3351
AjnI	CCWGG	7 cut(s)	1539, 1603, 1972, 2313, 3144, 3766, 3790
AleI	CACNNNNGTG	1 cut(s)	3620
AloI	GAACNNNNNNTCC	2 cut(s)	1398, 1430
Alw21I	GWGCWC	4 cut(s)	750, 1561, 2166, 3157
Alw26I	GTCTC	8 cut(s)	120, 140, 811, 1725, 1815, 2498, 2724, 3287
AlwI	GGATC	6 cut(s)	1293, 2259, 2685, 2956, 3648, 3737
AlwNI	CAGNNNCTG	2 cut(s)	2537, 3338
Ama87I	CYCGRG	1 cut(s)	382
AseI	ATTAAT	1 cut(s)	2931
Asp700I	GAANNNNTTC	1 cut(s)	785
AspLEI	GCGC	2 cut(s)	2616, 4082
AspS9I	GGNCC	5 cut(s)	2027, 2846, 2992, 3466, 3788
AsuC2I	CCSGG	4 cut(s)	31, 383, 384, 3469
AsuHPI	GGTGA	8 cut(s)	92, 97, 173, 590, 1310, 1445, 2954, 3911
AsuII	TTCGAA	1 cut(s)	1213
AsuNHI	GCTAGC	1 cut(s)	4064
AvaI	CYCGRG	1 cut(s)	382
AvaII	GGWCC	2 cut(s)	2027, 2846
AxyI	CCTNAGG	1 cut(s)	1815
BaeGI	GKGCMC	1 cut(s)	593
BaeI	ACNNNNGTAYC	2 cut(s)	930, 963
BanII	GRGCYC	3 cut(s)	750, 2166, 3906
BbsI	GAAGAC	2 cut(s)	2435, 3274
Bbv12I	GWGCWC	4 cut(s)	750, 1561, 2166, 3157
BbvCI	CCTCAGC	1 cut(s)	3579
BceAI	ACGGC	3 cut(s)	221, 1321, 2876
BcgI	CGANNNNNNTGC	6 cut(s)	659, 669, 693, 703, 1950, 1984
BciT130I	CCWGG	7 cut(s)	1541, 1605, 1974, 2315, 3146, 3768, 3792
BciVI	GTATCC	1 cut(s)	3641
BcnI	CCSGG	4 cut(s)	31, 383, 384, 3469
BcoDI	GTCTC	8 cut(s)	120, 140, 811, 1725, 1815, 2498, 2724, 3287
BfaI	CTAG	8 cut(s)	833, 1332, 2303, 2711, 3188, 3618, 3722, 4065
BfmI	CTRYAG	2 cut(s)	2529, 3363
BfoI	RGCGCY	1 cut(s)	2617
BfrI	CTTAAG	3 cut(s)	821, 1030, 1700
BfuAI	ACCTGC	1 cut(s)	3140
BfuI	GTATCC	1 cut(s)	3641
BglII	AGATCT	1 cut(s)	3475
BlpI	GCTNAGC	1 cut(s)	3441
BmcAI	AGTACT	1 cut(s)	831
Bme18I	GGWCC	2 cut(s)	2027, 2846
BmeT110I	CYCGRG	1 cut(s)	382
BmgT120I	GGNCC	5 cut(s)	2027, 2846, 2992, 3466, 3788
BmiI	GGNNCC	9 cut(s)	255, 308, 1538, 2029, 2334, 2994, 3312, 3405, 3772
BmrI	ACTGGG	2 cut(s)	2616, 2811
BmtI	GCTAGC	1 cut(s)	4068
BmuI	ACTGGG	2 cut(s)	2616, 2811
BoxI	GACNNNNGTC	1 cut(s)	132
BpiI	GAAGAC	2 cut(s)	2435, 3274
BpmI	CTGGAG	1 cut(s)	3624
Bpu10I	CCTNAGC	3 cut(s)	744, 3240, 3579
Bpu1102I	GCTNAGC	1 cut(s)	3441
Bpu14I	TTCGAA	1 cut(s)	1213
BpuEI	CTTGAG	8 cut(s)	134, 835, 1007, 1442, 1755, 2382, 2753, 2835
BpuMI	CCSGG	4 cut(s)	31, 383, 384, 3469
Bsa29I	ATCGAT	1 cut(s)	2180
BsaAI	YACGTR	2 cut(s)	283, 3398
BsaBI	GATNNNNATC	1 cut(s)	2250
BsaHI	GRCGYC	1 cut(s)	1247
BsaI	GGTCTC	2 cut(s)	140, 1815
BsaJI	CCNNGG	9 cut(s)	30, 381, 382, 1267, 1325, 1973, 1998, 2833, 3487
BsaXI	ACNNNNNCTCC	6 cut(s)	2085, 2115, 2386, 2416, 3351, 3381
Bsc4I	CCNNNNNNNGG	7 cut(s)	599, 1192, 1282, 1889, 2590, 3000, 3619
Bse118I	RCCGGY	1 cut(s)	1249
Bse1I	ACTGG	9 cut(s)	490, 604, 1161, 1528, 2564, 2611, 2817, 3535, 3607
Bse21I	CCTNAGG	1 cut(s)	1815
Bse3DI	GCAATG	3 cut(s)	1877, 2377, 2520
Bse8I	GATNNNNATC	1 cut(s)	2250
BseBI	CCWGG	7 cut(s)	1541, 1605, 1974, 2315, 3146, 3768, 3792
BseCI	ATCGAT	1 cut(s)	2180
BseDI	CCNNGG	9 cut(s)	30, 381, 382, 1267, 1325, 1973, 1998, 2833, 3487
BseJI	GATNNNNATC	1 cut(s)	2250
BseLI	CCNNNNNNNGG	7 cut(s)	599, 1192, 1282, 1889, 2590, 3000, 3619
BseMI	GCAATG	3 cut(s)	1877, 2377, 2520
BseNI	ACTGG	9 cut(s)	490, 604, 1161, 1528, 2564, 2611, 2817, 3535, 3607
BseRI	GAGGAG	4 cut(s)	38, 50, 2394, 3216
BseSI	GKGCMC	1 cut(s)	593
BseYI	CCCAGC	3 cut(s)	1109, 2167, 3661
BsgI	GTGCAG	3 cut(s)	212, 308, 1074
Bsh1236I	CGCG	2 cut(s)	1904, 4080
BshVI	ATCGAT	1 cut(s)	2180
BsiHKAI	GWGCWC	4 cut(s)	750, 1561, 2166, 3157
BsiHKCI	CYCGRG	1 cut(s)	382
BsiSI	CCGG	4 cut(s)	30, 383, 1250, 3469
BslFI	GGGAC	5 cut(s)	157, 1810, 2013, 2805, 3870
BslI	CCNNNNNNNGG	7 cut(s)	599, 1192, 1282, 1889, 2590, 3000, 3619
BsmAI	GTCTC	8 cut(s)	120, 140, 811, 1725, 1815, 2498, 2724, 3287
BsmFI	GGGAC	5 cut(s)	157, 1810, 2013, 2805, 3870
BsmI	GAATGC	1 cut(s)	1405
Bso31I	GGTCTC	2 cut(s)	140, 1815
BsoBI	CYCGRG	1 cut(s)	382
Bsp119I	TTCGAA	1 cut(s)	1213
Bsp1286I	GDGCHC	6 cut(s)	593, 750, 1561, 2166, 3157, 3906
Bsp1407I	TGTACA	1 cut(s)	1154
Bsp1720I	GCTNAGC	1 cut(s)	3441
Bsp19I	CCATGG	1 cut(s)	3487
BspACI	CCGC	8 cut(s)	1001, 1902, 2484, 2487, 2496, 2996, 3079, 4053
BspDI	ATCGAT	1 cut(s)	2180
BspFNI	CGCG	2 cut(s)	1904, 4080
BspHI	TCATGA	2 cut(s)	1060, 3138
BspLI	GGNNCC	9 cut(s)	255, 308, 1538, 2029, 2334, 2994, 3312, 3405, 3772
BspMAI	CTGCAG	2 cut(s)	2533, 3367
BspMI	ACCTGC	1 cut(s)	3140
BspOI	GCTAGC	1 cut(s)	4068
BspPI	GGATC	6 cut(s)	1293, 2259, 2685, 2956, 3648, 3737
BspQI	GCTCTTC	2 cut(s)	8, 2154
BspT104I	TTCGAA	1 cut(s)	1213
BspTI	CTTAAG	3 cut(s)	821, 1030, 1700
BspTNI	GGTCTC	2 cut(s)	140, 1815
BsrDI	GCAATG	3 cut(s)	1877, 2377, 2520
BsrFI	RCCGGY	1 cut(s)	1249
BsrGI	TGTACA	1 cut(s)	1154
BsrI	ACTGG	9 cut(s)	490, 604, 1161, 1528, 2564, 2611, 2817, 3535, 3607
BssAI	RCCGGY	1 cut(s)	1249
BssECI	CCNNGG	9 cut(s)	30, 381, 382, 1267, 1325, 1973, 1998, 2833, 3487
BssNAI	GTATAC	2 cut(s)	1937, 4025
BssNI	GRCGYC	1 cut(s)	1247
BssT1I	CCWWGG	3 cut(s)	1325, 1998, 3487
Bst1107I	GTATAC	2 cut(s)	1937, 4025
Bst2UI	CCWGG	7 cut(s)	1541, 1605, 1974, 2315, 3146, 3768, 3792
Bst4CI	ACNGT	9 cut(s)	187, 261, 292, 543, 647, 1277, 2585, 2984, 3184
Bst6I	CTCTTC	5 cut(s)	8, 15, 2154, 2727, 2910
BstACI	GRCGYC	1 cut(s)	1247
BstAFI	CTTAAG	3 cut(s)	821, 1030, 1700
BstAPI	GCANNNNNTGC	4 cut(s)	1862, 3223, 3338, 4061
BstAUI	TGTACA	1 cut(s)	1154
BstBAI	YACGTR	2 cut(s)	283, 3398
BstBI	TTCGAA	1 cut(s)	1213
BstDSI	CCRYGG	1 cut(s)	3487
BstEII	GGTNACC	1 cut(s)	1271
BstFNI	CGCG	2 cut(s)	1904, 4080
BstH2I	RGCGCY	1 cut(s)	2617
BstHHI	GCGC	2 cut(s)	2616, 4082
BstMAI	GTCTC	8 cut(s)	120, 140, 811, 1725, 1815, 2498, 2724, 3287
BstNI	CCWGG	7 cut(s)	1541, 1605, 1974, 2315, 3146, 3768, 3792
BstNSI	RCATGY	5 cut(s)	947, 1901, 3355, 3381, 3459
BstPAI	GACNNNNGTC	1 cut(s)	132
BstPI	GGTNACC	1 cut(s)	1271
BstSFI	CTRYAG	2 cut(s)	2529, 3363
BstSLI	GKGCMC	1 cut(s)	593
BstUI	CGCG	2 cut(s)	1904, 4080
BstV2I	GAAGAC	2 cut(s)	2435, 3274
BstX2I	RGATCY	6 cut(s)	1285, 2251, 2677, 3475, 3640, 3742
BstYI	RGATCY	6 cut(s)	1285, 2251, 2677, 3475, 3640, 3742
BstZ17I	GTATAC	2 cut(s)	1937, 4025
Bsu15I	ATCGAT	1 cut(s)	2180
Bsu36I	CCTNAGG	1 cut(s)	1815
BsuI	GTATCC	1 cut(s)	3641
BsuTUI	ATCGAT	1 cut(s)	2180
BtgI	CCRYGG	1 cut(s)	3487
BtgZI	GCGATG	1 cut(s)	2150
BtsI	GCAGTG	3 cut(s)	2350, 2823, 3049
BtsIMutI	CAGTG	9 cut(s)	257, 297, 483, 796, 1521, 2350, 2823, 2980, 3049
BveI	ACCTGC	1 cut(s)	3140
CaiI	CAGNNNCTG	2 cut(s)	2537, 3338
CciI	TCATGA	2 cut(s)	1060, 3138
CfoI	GCGC	2 cut(s)	2616, 4082
Cfr10I	RCCGGY	1 cut(s)	1249
Cfr13I	GGNCC	5 cut(s)	2027, 2846, 2992, 3466, 3788
Cfr9I	CCCGGG	1 cut(s)	382
ClaI	ATCGAT	1 cut(s)	2180
CseI	GACGC	2 cut(s)	1255, 3324
CsiI	ACCWGGT	1 cut(s)	3766
DraIII	CACNNNGTG	1 cut(s)	368
Eam1104I	CTCTTC	5 cut(s)	8, 15, 2154, 2727, 2910
EarI	CTCTTC	5 cut(s)	8, 15, 2154, 2727, 2910
EciI	GGCGGA	1 cut(s)	3094
Ecl136II	GAGCTC	2 cut(s)	748, 2164
Eco130I	CCWWGG	3 cut(s)	1325, 1998, 3487
Eco147I	AGGCCT	2 cut(s)	321, 1698
Eco24I	GRGCYC	3 cut(s)	750, 2166, 3906
Eco31I	GGTCTC	2 cut(s)	140, 1815
Eco32I	GATATC	1 cut(s)	636
Eco47I	GGWCC	2 cut(s)	2027, 2846
Eco53kI	GAGCTC	2 cut(s)	748, 2164
Eco57I	CTGAAG	6 cut(s)	236, 992, 1823, 2043, 3872, 3990
Eco81I	CCTNAGG	1 cut(s)	1815
Eco88I	CYCGRG	1 cut(s)	382
Eco91I	GGTNACC	1 cut(s)	1271
EcoICRI	GAGCTC	2 cut(s)	748, 2164
EcoO65I	GGTNACC	1 cut(s)	1271
EcoRI	GAATTC	2 cut(s)	69, 1407
EcoRII	CCWGG	7 cut(s)	1539, 1603, 1972, 2313, 3144, 3766, 3790
EcoRV	GATATC	1 cut(s)	636
EcoT14I	CCWWGG	3 cut(s)	1325, 1998, 3487
EcoT22I	ATGCAT	3 cut(s)	409, 1459, 3379
EcoT38I	GRGCYC	3 cut(s)	750, 2166, 3906
ErhI	CCWWGG	3 cut(s)	1325, 1998, 3487
FalI	AAGNNNNNCTT	4 cut(s)	2500, 2532, 2630, 2662
FaqI	GGGAC	5 cut(s)	157, 1810, 2013, 2805, 3870
FauI	CCCGC	1 cut(s)	3003
FauNDI	CATATG	1 cut(s)	2382
FblI	GTMKAC	3 cut(s)	1936, 3168, 4024
FriOI	GRGCYC	3 cut(s)	750, 2166, 3906
FspBI	CTAG	8 cut(s)	833, 1332, 2303, 2711, 3188, 3618, 3722, 4065
GlaI	GCGC	2 cut(s)	2615, 4081
GsaI	CCCAGC	3 cut(s)	1113, 2171, 3665
GsuI	CTGGAG	1 cut(s)	3624
HaeII	RGCGCY	1 cut(s)	2617
HapII	CCGG	4 cut(s)	30, 383, 1250, 3469
HgaI	GACGC	2 cut(s)	1255, 3324
HhaI	GCGC	2 cut(s)	2616, 4082
Hin1I	GRCGYC	1 cut(s)	1247
Hin6I	GCGC	2 cut(s)	2614, 4080
HinP1I	GCGC	2 cut(s)	2614, 4080
HincII	GTYRAC	1 cut(s)	2041
HindII	GTYRAC	1 cut(s)	2041
HindIII	AAGCTT	2 cut(s)	149, 4085
HpaII	CCGG	4 cut(s)	30, 383, 1250, 3469
HphI	GGTGA	8 cut(s)	92, 97, 173, 590, 1310, 1445, 2954, 3911
Hpy166II	GTNNAC	8 cut(s)	183, 605, 1712, 1937, 2041, 2146, 3169, 4025
Hpy8I	GTNNAC	8 cut(s)	183, 605, 1712, 1937, 2041, 2146, 3169, 4025
Hpy99I	CGWCG	2 cut(s)	78, 1249
HpyCH4III	ACNGT	9 cut(s)	187, 261, 292, 543, 647, 1277, 2585, 2984, 3184
HpyCH4IV	ACGT	7 cut(s)	282, 1051, 2148, 2283, 3397, 3822, 4021
HpySE526I	ACGT	7 cut(s)	282, 1051, 2148, 2283, 3397, 3822, 4021
Hsp92I	GRCGYC	1 cut(s)	1247
HspAI	GCGC	2 cut(s)	2614, 4080
KroI	GCCGGC	1 cut(s)	1249
KroNI	GCCGGC	1 cut(s)	1251
LguI	GCTCTTC	2 cut(s)	8, 2154
MabI	ACCWGGT	1 cut(s)	3766
MaeI	CTAG	8 cut(s)	833, 1332, 2303, 2711, 3188, 3618, 3722, 4065
MaeII	ACGT	7 cut(s)	282, 1051, 2148, 2283, 3397, 3822, 4021
MfeI	CAATTG	4 cut(s)	498, 1392, 1944, 3666
MflI	RGATCY	6 cut(s)	1285, 2251, 2677, 3475, 3640, 3742
MhlI	GDGCHC	6 cut(s)	593, 750, 1561, 2166, 3157, 3906
MlyI	GAGTC	2 cut(s)	47, 87
MmeI	TCCRAC	1 cut(s)	1514
Mph1103I	ATGCAT	3 cut(s)	409, 1459, 3379
MroNI	GCCGGC	1 cut(s)	1249
MroXI	GAANNNNTTC	1 cut(s)	785
MslI	CAYNNNNRTG	5 cut(s)	780, 2272, 2594, 3620, 3864
MspA1I	CMGCKG	4 cut(s)	1681, 1802, 2012, 3926
MspCI	CTTAAG	3 cut(s)	821, 1030, 1700
MspI	CCGG	4 cut(s)	30, 383, 1250, 3469
MunI	CAATTG	4 cut(s)	498, 1392, 1944, 3666
Mva1269I	GAATGC	1 cut(s)	1405
MvaI	CCWGG	7 cut(s)	1541, 1605, 1974, 2315, 3146, 3768, 3792
MvnI	CGCG	2 cut(s)	1904, 4080
NaeI	GCCGGC	1 cut(s)	1251
NciI	CCSGG	4 cut(s)	31, 383, 384, 3469
NcoI	CCATGG	1 cut(s)	3487
NdeI	CATATG	1 cut(s)	2382
NgoMIV	GCCGGC	1 cut(s)	1249
NheI	GCTAGC	1 cut(s)	4064
NlaIV	GGNNCC	9 cut(s)	255, 308, 1538, 2029, 2334, 2994, 3312, 3405, 3772
NmuCI	GTSAC	6 cut(s)	647, 665, 2068, 2276, 2585, 3190
NsiI	ATGCAT	3 cut(s)	409, 1459, 3379
NspI	RCATGY	5 cut(s)	947, 1901, 3355, 3381, 3459
NspV	TTCGAA	1 cut(s)	1213
OliI	CACNNNNGTG	1 cut(s)	3620
PaeI	GCATGC	2 cut(s)	1901, 3459
PagI	TCATGA	2 cut(s)	1060, 3138
PaqCI	CACCTGC	1 cut(s)	3140
PceI	AGGCCT	2 cut(s)	321, 1698
PciI	ACATGT	2 cut(s)	943, 3351
PciSI	GCTCTTC	2 cut(s)	8, 2154
PctI	GAATGC	1 cut(s)	1405
PdiI	GCCGGC	1 cut(s)	1251
PdmI	GAANNNNTTC	1 cut(s)	785
PfeI	GAWTC	8 cut(s)	338, 786, 967, 1223, 1952, 2557, 2770, 2878
PflFI	GACNNNGTC	1 cut(s)	2282
PleI	GAGTC	2 cut(s)	46, 86
PpsI	GAGTC	2 cut(s)	46, 86
Ppu21I	YACGTR	2 cut(s)	283, 3398
PscI	ACATGT	2 cut(s)	943, 3351
PshAI	GACNNNNGTC	1 cut(s)	132
PshBI	ATTAAT	1 cut(s)	2931
Psp124BI	GAGCTC	2 cut(s)	750, 2166
Psp6I	CCWGG	7 cut(s)	1539, 1603, 1972, 2313, 3144, 3766, 3790
PspEI	GGTNACC	1 cut(s)	1271
PspFI	CCCAGC	3 cut(s)	1109, 2167, 3661
PspGI	CCWGG	7 cut(s)	1539, 1603, 1972, 2313, 3144, 3766, 3790
PspN4I	GGNNCC	9 cut(s)	255, 308, 1538, 2029, 2334, 2994, 3312, 3405, 3772
PspPI	GGNCC	5 cut(s)	2027, 2846, 2992, 3466, 3788
PsrI	GAACNNNNNNTAC	2 cut(s)	595, 627
PstI	CTGCAG	2 cut(s)	2533, 3367
PstNI	CAGNNNCTG	2 cut(s)	2537, 3338
PsuI	RGATCY	6 cut(s)	1285, 2251, 2677, 3475, 3640, 3742
PsyI	GACNNNGTC	1 cut(s)	2282
PvuII	CAGCTG	4 cut(s)	1681, 1802, 2012, 3926
RseI	CAYNNNNRTG	5 cut(s)	780, 2272, 2594, 3620, 3864
SacI	GAGCTC	2 cut(s)	750, 2166
SapI	GCTCTTC	2 cut(s)	8, 2154
Sau96I	GGNCC	5 cut(s)	2027, 2846, 2992, 3466, 3788
ScaI	AGTACT	1 cut(s)	831
SchI	GAGTC	2 cut(s)	47, 87
SduI	GDGCHC	6 cut(s)	593, 750, 1561, 2166, 3157, 3906
SexAI	ACCWGGT	1 cut(s)	3766
SfcI	CTRYAG	2 cut(s)	2529, 3363
SfuI	TTCGAA	1 cut(s)	1213
SinI	GGWCC	2 cut(s)	2027, 2846
SmaI	CCCGGG	1 cut(s)	384
SmiMI	CAYNNNNRTG	5 cut(s)	780, 2272, 2594, 3620, 3864
SphI	GCATGC	2 cut(s)	1901, 3459
SseBI	AGGCCT	2 cut(s)	321, 1698
SsiI	CCGC	8 cut(s)	1001, 1902, 2484, 2487, 2496, 2996, 3079, 4053
SspI	AATATT	3 cut(s)	1911, 2955, 3265
SspMI	CTAG	8 cut(s)	833, 1332, 2303, 2711, 3188, 3618, 3722, 4065
SstI	GAGCTC	2 cut(s)	750, 2166
StuI	AGGCCT	2 cut(s)	321, 1698
StyI	CCWWGG	3 cut(s)	1325, 1998, 3487
TaaI	ACNGT	9 cut(s)	187, 261, 292, 543, 647, 1277, 2585, 2984, 3184
TaiI	ACGT	7 cut(s)	285, 1054, 2151, 2286, 3400, 3825, 4024
TaqI	TCGA	8 cut(s)	41, 76, 478, 1213, 2180, 2560, 3513, 3684
TatI	WGTACW	3 cut(s)	182, 829, 1154
TauI	GCSGC	3 cut(s)	1904, 2487, 2499
TfiI	GAWTC	8 cut(s)	338, 786, 967, 1223, 1952, 2557, 2770, 2878
TscAI	CASTG	9 cut(s)	264, 297, 490, 796, 1528, 2350, 2830, 2987, 3049
TseFI	GTSAC	6 cut(s)	647, 665, 2068, 2276, 2585, 3190
Tsp45I	GTSAC	6 cut(s)	647, 665, 2068, 2276, 2585, 3190
TspGWI	ACGGA	4 cut(s)	388, 734, 2606, 4042
TspMI	CCCGGG	1 cut(s)	382
TspRI	CASTG	9 cut(s)	264, 297, 490, 796, 1528, 2350, 2830, 2987, 3049
Tth111I	GACNNNGTC	1 cut(s)	2282
Vha464I	CTTAAG	3 cut(s)	821, 1030, 1700
VpaK11BI	GGWCC	2 cut(s)	2027, 2846
VspI	ATTAAT	1 cut(s)	2931
XceI	RCATGY	5 cut(s)	947, 1901, 3355, 3381, 3459
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2624
XmaI	CCCGGG	1 cut(s)	382
XmiI	GTMKAC	3 cut(s)	1936, 3168, 4024
XmnI	GAANNNNTTC	1 cut(s)	785
XspI	CTAG	8 cut(s)	833, 1332, 2303, 2711, 3188, 3618, 3722, 4065
ZrmI	AGTACT	1 cut(s)	831
Zsp2I	ATGCAT	3 cut(s)	409, 1459, 3379

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.