Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr7

Orthogroup ID

OG0005756

Physical Location & Seq

Genomic Coordinates Forward (+)

56270760 .. 56282285

Gene Structure 11526 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000034866

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4101 bp

ATGGGGTCGTTGTTGAAGAGCGAAGAGGAGGCCGGGGAGGAGTCGAACGAGGCCATATTGTATGTTAATGGAATTCGTCGAGTCTTGCCTGATGGATTGGCTCACCTCACCCTCCTTGAGTACCTCAGAGATATAGGTCTCACGGGGACAAAGCTTGGTTGTGGTGAAGGTGGTTGTGGTGCGTGCACTGTAATGGTTTCGCATTATGACCAAAAACTGAAGAAATGCCTGCACTACGCCGTTAATGCATGCTTGGCTCCACTGTATTCTTTAGAAGGGATGCACGTAATTACAGTGGAAGGACTTGGGAACCATAAACAAGGCCTGCACCCAATTCAAGAATCATTGGCACAATCTCATGGCTCACAGTGTGGATTTTGCACCCCGGGTTTTATTATGTCCGTCTATGCATTATTAAGGTCAAGTCAGACCTCACCTAATGAGGAGCAGATTGAAGAATGTTTAGCAGGAAACTTGTGTCGATGCACTGGTTACAGACCAATTGTTGATGCTTTTCGTGTCTTTGCCAAAACAGATGATAAACCCTACATTGACATATCTTCTCTAAGCCTTGAAGGAGGTGAATTTGTGTGCCCTTCAACTGGTAAACCCTGTTCGTGTGGATTAAAAAGTGAGATATCAAGCAACCGTCACAAAACGGGTACTTGTGACGAGAGGTATGCACCTGTATCGTATAGTGAAATTAATGGAAGCACATACACGGACAAGGAACTTATATTTCCTCCTGAGCTCATATTGAGAAAATCAACTTATTTACATTTGAATGGATTCAGTGGGTTAAAGTGGTTCAGACCCTTGAGACTTAAGCAAGTACTAGAGTTAAAAGAAAAATACCCAGATGCAAAGTTATTGGTAGGTAATACTGAGGTAGGAATTGAAATGAGGTTGAAAAAAATTCAATATCAGGTTTTAATTTCTGTTACACATGTACCTGAACTTAGTATACTGAATCTGAAGGATGATGGGCTTGAGATAGGTTCTGCAGTAAGACTTTCTGAACTACTTAAATTCTTAAGAAAGGTCATAACAGAACGTGCAGCTCATGAAACTTCTTCCTGTAAGGCTTTTGTTGAACAATTAAAATGGTTTGCCGGGATGCAAATAAGAAATGTTGCTTGTGTGGGTGGAAATATCTGTACAGCCAGTCCAATTTCAGACTTGAACCCCCTTTGGATGGCTGCCAAAGCAACTTTTCGAATTGTTGATTCCAAAGGGAACATTAGAACGACGCTGGCAGAAAAGTTTTTCCTCGGTTACCGTAAGGTGGATCTGGGAAGTGGTGAAATTCTACTTTCTGTATTTTTACCTTGGACTAGGCCGTTTGAGTATGTGAAAGAATATAAACAAGCTCACAGAAGGGATGATGATATTGCAATTGTGAATGCTGGAATTCGTGTTCATCTTGAGGAAAGAGGTGAAAACATTGTTGTTTCTGATGCATCTATTGTTTATGGGGGTGTAGCTCCACTCTCTCTTTCTGCAACAAGAACAAAAGACTTTCTCACTGGAAAGAGTTGGAACCAGGAGCTATTGCAGGGTGCTCTGAAAATCATCCAGAAGGATGTATTGCTCAGAGATAATGCTCCTGGTGGGATGGTGGAGTTTCGTAAGTCTTTGACACTTAGCTTTTTCTTCAAGTTTTTTCTGTGGGTTTCTCATCAGCTGGAAAGGGAAAAAGGCCTTAAGGGGAGTGTACCATTGTCTCATCTTTCTGCTATACAACCATTTGACAGGCCATCTGTTATAGGAACTCAAGACTATGAAATTACAAAACATGGGACAGCTGTTGGGTCTCCTGAGGTTCATCTATCATCGAGACTTCAGGTTTCAGGAGAGGCAGAATATGCTGATGATACACCATTGCCTCCCAATGGCTTGCATGCCGCGTTAATATTGAGCAAAAAACCTCATGCTCGTATACTTTCAATTGATGATTCAGGAGCAAAGTTATCGCCTGGGTTTGCAGGCATATTTTTTGCCAAGGATGTTCCAGCTGATAATAAAGTTGGACCCGTTGTTGTTGACGAGGAACTATTTGCTTCAGAGTTTGTCACTTGTGTAGGTCAGGTTATAGGAGTGGTAGTAGCAGATACACATGAAAAGGCAAAATTAGCAGCAATGAAAGTTCACGTTGAGTATGAAGAGCTCCCAGCCATCTTATCGATACAGGATGCTATCAATGCTAATAGTTTCCATCCTAATACTGAGAGGCGATTTAGGAAAGGAGATGTGGATCTTTGCTTTCAATCATGTCAATGTGACAAGGTCATAGAGGGGGAAGTTCTAGTGGGAGGACAGGAACACTTCTATTTGGAGCCACAGAGCAGTGTGATATGGACAATGGATGGTGGCAATGAAGTTCATATGATCTCATCCACTCAAGCTCCTCAGAAGCACCAGAAGTATGTTTCTCATGTTCTTGGTCTTCCAATGTCAAAAGTAATTTGTAAAACGAAGCGTATTGGTGGCGGTTTTGGCGGCAAGGAGACTAGATCAGCTTTCATTGCTGCTGCAGCTTCTGTTCCTTCATATCTATTGAATCGACCAGTCAAAATTACATTAGACCGTGATACGGACATGATGATAACTGGGCAGCGCCATAGCTTTCTTGGAAAATACAAGGTGGGCTTTACAAATGAGGGAAAGGTGTTGGCATTGGATGTTCACATCTATAATAGTGCTGGAAATTCACTAGACTTGTCTCTTCCTGTGCTTGAGCGTGCTATGTTTCACTCGGATAATGTCTATGAGATTCCAAATGTGAGAATTGTGGGACGAGTTTGCTTCACTAATATACCCAGTAACACTGCTTTCCGAGGATTTGGTGGTCCTCAAGGGATGATTATTGCTGAAAATTGGATTCAAAGAATTGCCGTAGAACTGAATAAAAGCCCAGAAGAGATAAGAGAGATTAATTTTCAAGGTGAAGGATCAATATTGCATTATGGTCAGAAACTTCAACACTGTACACTGGCCCCGCTTTGGAACGAACTAAAACTATCTTGTGAATTTTCAAAGGCTCGCAGTGAAGTTTGCCAATTTAATACTCATAATCGGTGGAGGAAGCGTGGTGTAGCCATGATACCAACGAAGTTTGGCATATCATTTACATTAAAGCTCATGAACCAGGCAGGTGCTCTTGTTCATGTCTACACAGATGGAACTGTTCTAGTCACACATGGAGGAGTTGAGATGGGGCAAGGTTTGCATACAAAAGTTGCTCAGGTGGCTGCTTCTGCCTTCAATATTCCTCTCAGTTCAGTCTTCATATCAGAGACAAGTACCGACAAGGTTCCTAATGCTTCCCCAACAGCAGCGTCTGCAAGTTCTGACATGTATGGAGCTGCAGTCTTGGATGCATGTGAGCAAATCAAGGCACGTATGGAACCTATTGCTTCAGAGCACAATTTCAGCTCTTTTGCTGAGCTGGCAAGTGCATGCTATGAGGCCCGGATAGATCTTTCTGCCCATGGATTTTACATTGTACCTGAAATCGACTTTGATTGGACTACTGGTAAAGGCAACCCATTCAGGTACTTTACCTATGGGGCTGCTTTTGCTGAGGTTGAAATTGACACTTTGACTGGAGATTTCCACACTAGGGTGGCAAATATATTCTTGGATCTTGGATACTCTCTCAACCCAGCAATTGATGTTGGGCAGATCGAAGGAGCGTTTATACAAGGTTTGGGATGGGTTGCCCTAGAAGAACTAAAATGGGGAGATCCTGCTCATAAGTGGATTGCACCAGGTTCCCTCTACACTTGTGGGCCTGGAAGCTACAAAATTCCTTCCATAAATGACGTTCCCTTAAAATTCAATGTTTCACTTCTGAAGGGACATCCAAATGTAAAGGCCATCCACTCATCTAAAGCTGTTGGTGAGCCCCCATTTTTCTTGGCATCGGCTGTCTTTTTTGCTATCAAAGATGCCATTATAGCTGCAAGAGCTGAAGTAGGGTGTAACGAGTGGTTTCCACTTGATAATCCAGCAACACCAGAACGTATACGGATGGCTTGTTTGGATGAATTTACCGCAGCATTTGCTACCTCAGATTTTCGCGCAAAGCTTAGTGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0000302 GO:0001101 GO:0003674 GO:0003824 GO:0004854 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006139 GO:0006144 GO:0006145 GO:0006725 GO:0006801 GO:0006807 GO:0006950 GO:0006979 GO:0008150 GO:0008152 GO:0009056 GO:0009112 GO:0009115 GO:0009414 GO:0009415 GO:0009628 GO:0009987 GO:0010035 GO:0016491 GO:0016725 GO:0016726 GO:0016903 GO:0019439 GO:0034641 GO:0036094 GO:0042221 GO:0042554 GO:0043167 GO:0043168 GO:0043546 GO:0044237 GO:0044238 GO:0044248 GO:0044270 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046110 GO:0046113 GO:0046483 GO:0046700 GO:0048037 GO:0050660 GO:0050662 GO:0050896 GO:0051536 GO:0051537 GO:0051540 GO:0055086 GO:0055114 GO:0071704 GO:0072521 GO:0072523 GO:0072593 GO:0097159 GO:1901265 GO:1901360 GO:1901361 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00230 ko00232 ko00983 ko01100 ko01110 ko01120 ko04146 map00230 map00232 map00983 map01100 map01110 map01120 map04146

Pfam Domains

Protein Families

Ald_Xan_dh_C Ald_Xan_dh_C2 CO_deh_flav_C FAD_binding_5 Fer2 Fer2_2

Protein Analysis

1367

Amino Acids

149.99

Weight (kDa)

6.37

Isoelectric Point (pI)

38.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Fer2	PF00111	35 - 87	1.1e-07	2Fe-2S iron-sulfur cluster binding domain
Fer2_2	PF01799	98 - 172	4.2e-32	[2Fe-2S] binding domain
FAD_binding_5	PF00941	267 - 444	3e-49	FAD binding domain in molybdopterin dehydrogenase
CO_deh_flav_C	PF03450	453 - 558	7.4e-29	CO dehydrogenase flavoprotein C-terminal domain
Ald_Xan_dh_C	PF01315	617 - 724	1.1e-28	Aldehyde oxidase and xanthine dehydrogenase, a/b hammerhead domain
MoCoBD_1	PF02738	740 - 980	7.3e-87	Molybdopterin cofactor-binding domain
MoCoBD_2	PF20256	1008 - 1277	3e-88	Molybdopterin cofactor-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0005756)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G34890 AT4G34900 AT4G34900 AT4G34900
fragaria_vesca	FvH4_3g29000 FvH4_3g29000 FvH4_3g29000 FvH4_3g29000
malus_domestica	MD03G1177200.v1.1 MD11G1161000.v1.1 MD11G1197900.v1.1
prunus_persica	Prupe.4G245700_v2.0.a1 Prupe.4G245700_v2.0.a1
pyrus_communis	pycom11g13410 pycom11g17110 pycom11g17120
rosa_chinensis	RchiOBHm_Chr5g0051661
rosa_laevigata	RLG00000034866
rosa_multiflora	Rmu_sc0000068.1_g000026 Rmu_sc0014882.1_g000001 Rmu_sc0018333.1_g000002
rosa_roxburghii	Rroxscaffold_1G00027170
rosa_rugosa	Rorug05G0277900
rosa_samantha	Rh5AG349700 Rh5BG361400 Rh5CG385900 Rh5DG376200
rosa_wichuraiana	Rw5G033070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	3143
Acc36I	ACCTGC	1 cut(s)	3143
AccI	GTMKAC	4 cut(s)	964, 1939, 3171, 4027
AccII	CGCG	2 cut(s)	1907, 4083
AciI	CCGC	5 cut(s)	1905, 2490, 2499, 2999, 4056
AclWI	GGATC	5 cut(s)	1296, 2262, 2959, 3651, 3740
AcuI	CTGAAG	7 cut(s)	239, 995, 1826, 2046, 3402, 3875, 3993
AdeI	CACNNNGTG	1 cut(s)	371
AfiI	CCNNNNNNNGG	7 cut(s)	602, 1195, 1285, 1892, 2593, 3003, 3622
AflII	CTTAAG	3 cut(s)	824, 1033, 1703
AflIII	ACRYGT	2 cut(s)	946, 3354
AjnI	CCWGG	6 cut(s)	1542, 1606, 1975, 3147, 3769, 3793
AleI	CACNNNNGTG	1 cut(s)	3623
AloI	GAACNNNNNNTCC	2 cut(s)	1401, 1433
Alw21I	GWGCWC	6 cut(s)	188, 753, 1564, 2169, 3160, 3426
Alw26I	GTCTC	8 cut(s)	143, 814, 1728, 1818, 1831, 2501, 2727, 3290
Alw44I	GTGCAC	1 cut(s)	184
AlwI	GGATC	5 cut(s)	1296, 2262, 2959, 3651, 3740
AlwNI	CAGNNNCTG	2 cut(s)	2540, 3341
Ama87I	CYCGRG	1 cut(s)	385
ApaLI	GTGCAC	1 cut(s)	184
AseI	ATTAAT	2 cut(s)	705, 2934
Asp700I	GAANNNNTTC	1 cut(s)	788
AspLEI	GCGC	2 cut(s)	2619, 4085
AspS9I	GGNCC	5 cut(s)	2030, 2849, 2995, 3469, 3791
AsuC2I	CCSGG	5 cut(s)	34, 386, 387, 1114, 3472
AsuHPI	GGTGA	9 cut(s)	95, 100, 176, 426, 593, 1313, 1448, 2957, 3914
AsuII	TTCGAA	1 cut(s)	1216
AvaI	CYCGRG	1 cut(s)	385
AvaII	GGWCC	2 cut(s)	2030, 2849
AxyI	CCTNAGG	1 cut(s)	1818
BaeGI	GKGCMC	2 cut(s)	188, 596
BaeI	ACNNNNGTAYC	2 cut(s)	933, 966
BanII	GRGCYC	3 cut(s)	753, 2169, 3909
BbsI	GAAGAC	2 cut(s)	2438, 3277
Bbv12I	GWGCWC	6 cut(s)	188, 753, 1564, 2169, 3160, 3426
BbvCI	CCTCAGC	1 cut(s)	3582
BceAI	ACGGC	3 cut(s)	224, 1324, 2879
BcgI	CGANNNNNNTGC	6 cut(s)	662, 672, 696, 706, 1953, 1987
BciT130I	CCWGG	6 cut(s)	1544, 1608, 1977, 3149, 3771, 3795
BciVI	GTATCC	1 cut(s)	3644
BcnI	CCSGG	5 cut(s)	34, 386, 387, 1114, 3472
BcoDI	GTCTC	8 cut(s)	143, 814, 1728, 1818, 1831, 2501, 2727, 3290
BfaI	CTAG	8 cut(s)	836, 1335, 2306, 2511, 2714, 3191, 3621, 3725
BfmI	CTRYAG	3 cut(s)	1002, 2532, 3366
BfoI	RGCGCY	1 cut(s)	2620
BfrI	CTTAAG	3 cut(s)	824, 1033, 1703
BfuAI	ACCTGC	1 cut(s)	3143
BfuI	GTATCC	1 cut(s)	3644
BglII	AGATCT	1 cut(s)	3478
BlpI	GCTNAGC	1 cut(s)	3444
BmcAI	AGTACT	1 cut(s)	834
Bme18I	GGWCC	2 cut(s)	2030, 2849
BmeT110I	CYCGRG	1 cut(s)	385
BmgT120I	GGNCC	5 cut(s)	2030, 2849, 2995, 3469, 3791
BmiI	GGNNCC	9 cut(s)	258, 311, 1541, 2032, 2337, 2997, 3315, 3408, 3775
BmrI	ACTGGG	2 cut(s)	2619, 2814
BmuI	ACTGGG	2 cut(s)	2619, 2814
BpiI	GAAGAC	2 cut(s)	2438, 3277
BpmI	CTGGAG	1 cut(s)	3627
Bpu10I	CCTNAGC	3 cut(s)	747, 3243, 3582
Bpu1102I	GCTNAGC	1 cut(s)	3444
Bpu14I	TTCGAA	1 cut(s)	1216
BpuEI	CTTGAG	8 cut(s)	137, 838, 1010, 1445, 1758, 2385, 2756, 2838
BpuMI	CCSGG	5 cut(s)	34, 386, 387, 1114, 3472
Bsa29I	ATCGAT	1 cut(s)	2183
BsaAI	YACGTR	2 cut(s)	286, 3401
BsaBI	GATNNNNATC	1 cut(s)	2253
BsaI	GGTCTC	2 cut(s)	143, 1818
BsaJI	CCNNGG	9 cut(s)	33, 384, 385, 1270, 1328, 1976, 2001, 2836, 3490
BsaXI	ACNNNNNCTCC	8 cut(s)	2088, 2118, 2389, 2419, 3073, 3103, 3354, 3384
Bsc4I	CCNNNNNNNGG	7 cut(s)	602, 1195, 1285, 1892, 2593, 3003, 3622
Bse21I	CCTNAGG	1 cut(s)	1818
Bse3DI	GCAATG	4 cut(s)	1880, 2145, 2380, 2523
Bse8I	GATNNNNATC	1 cut(s)	2253
BseBI	CCWGG	6 cut(s)	1544, 1608, 1977, 3149, 3771, 3795
BseCI	ATCGAT	1 cut(s)	2183
BseDI	CCNNGG	9 cut(s)	33, 384, 385, 1270, 1328, 1976, 2001, 2836, 3490
BseJI	GATNNNNATC	1 cut(s)	2253
BseLI	CCNNNNNNNGG	7 cut(s)	602, 1195, 1285, 1892, 2593, 3003, 3622
BseMI	GCAATG	4 cut(s)	1880, 2145, 2380, 2523
BseRI	GAGGAG	5 cut(s)	41, 53, 458, 2397, 3219
BseSI	GKGCMC	2 cut(s)	188, 596
BseYI	CCCAGC	2 cut(s)	2170, 3664
BsgI	GTGCAG	3 cut(s)	215, 311, 1077
Bsh1236I	CGCG	2 cut(s)	1907, 4083
BshVI	ATCGAT	1 cut(s)	2183
BsiHKAI	GWGCWC	6 cut(s)	188, 753, 1564, 2169, 3160, 3426
BsiHKCI	CYCGRG	1 cut(s)	385
BsiSI	CCGG	4 cut(s)	33, 386, 1113, 3472
BslFI	GGGAC	4 cut(s)	160, 1813, 2808, 3873
BslI	CCNNNNNNNGG	7 cut(s)	602, 1195, 1285, 1892, 2593, 3003, 3622
BsmAI	GTCTC	8 cut(s)	143, 814, 1728, 1818, 1831, 2501, 2727, 3290
BsmFI	GGGAC	4 cut(s)	160, 1813, 2808, 3873
BsmI	GAATGC	1 cut(s)	1408
Bso31I	GGTCTC	2 cut(s)	143, 1818
BsoBI	CYCGRG	1 cut(s)	385
Bsp119I	TTCGAA	1 cut(s)	1216
Bsp1286I	GDGCHC	8 cut(s)	188, 596, 753, 1564, 2169, 3160, 3426, 3909
Bsp1407I	TGTACA	2 cut(s)	1157, 2987
Bsp143I	GATC	9 cut(s)	1288, 2254, 2388, 2513, 2951, 3478, 3643, 3684, 3745
Bsp1720I	GCTNAGC	1 cut(s)	3444
Bsp19I	CCATGG	1 cut(s)	3490
BspACI	CCGC	5 cut(s)	1905, 2490, 2499, 2999, 4056
BspDI	ATCGAT	1 cut(s)	2183
BspFNI	CGCG	2 cut(s)	1907, 4083
BspHI	TCATGA	2 cut(s)	1063, 3141
BspLI	GGNNCC	9 cut(s)	258, 311, 1541, 2032, 2337, 2997, 3315, 3408, 3775
BspMAI	CTGCAG	3 cut(s)	1006, 2536, 3370
BspMI	ACCTGC	1 cut(s)	3143
BspPI	GGATC	5 cut(s)	1296, 2262, 2959, 3651, 3740
BspQI	GCTCTTC	2 cut(s)	11, 2157
BspT104I	TTCGAA	1 cut(s)	1216
BspTI	CTTAAG	3 cut(s)	824, 1033, 1703
BspTNI	GGTCTC	2 cut(s)	143, 1818
BsrDI	GCAATG	4 cut(s)	1880, 2145, 2380, 2523
BsrGI	TGTACA	2 cut(s)	1157, 2987
BssECI	CCNNGG	9 cut(s)	33, 384, 385, 1270, 1328, 1976, 2001, 2836, 3490
BssMI	GATC	9 cut(s)	1288, 2254, 2388, 2513, 2951, 3478, 3643, 3684, 3745
BssNAI	GTATAC	3 cut(s)	965, 1940, 4028
BssT1I	CCWWGG	3 cut(s)	1328, 2001, 3490
Bst1107I	GTATAC	3 cut(s)	965, 1940, 4028
Bst2UI	CCWGG	6 cut(s)	1544, 1608, 1977, 3149, 3771, 3795
Bst4CI	ACNGT	9 cut(s)	190, 264, 295, 369, 650, 1280, 2588, 2987, 3187
Bst6I	CTCTTC	5 cut(s)	11, 18, 2157, 2730, 2913
BstAFI	CTTAAG	3 cut(s)	824, 1033, 1703
BstAPI	GCANNNNNTGC	4 cut(s)	1865, 3226, 3341, 4064
BstAUI	TGTACA	2 cut(s)	1157, 2987
BstBAI	YACGTR	2 cut(s)	286, 3401
BstBI	TTCGAA	1 cut(s)	1216
BstDSI	CCRYGG	1 cut(s)	3490
BstEII	GGTNACC	1 cut(s)	1274
BstFNI	CGCG	2 cut(s)	1907, 4083
BstH2I	RGCGCY	1 cut(s)	2620
BstHHI	GCGC	2 cut(s)	2619, 4085
BstKTI	GATC	9 cut(s)	1291, 2257, 2391, 2516, 2954, 3481, 3646, 3687, 3748
BstMAI	GTCTC	8 cut(s)	143, 814, 1728, 1818, 1831, 2501, 2727, 3290
BstMBI	GATC	9 cut(s)	1288, 2254, 2388, 2513, 2951, 3478, 3643, 3684, 3745
BstNI	CCWGG	6 cut(s)	1544, 1608, 1977, 3149, 3771, 3795
BstNSI	RCATGY	6 cut(s)	252, 950, 1904, 3358, 3384, 3462
BstPI	GGTNACC	1 cut(s)	1274
BstSFI	CTRYAG	3 cut(s)	1002, 2532, 3366
BstSLI	GKGCMC	2 cut(s)	188, 596
BstUI	CGCG	2 cut(s)	1907, 4083
BstV2I	GAAGAC	2 cut(s)	2438, 3277
BstX2I	RGATCY	5 cut(s)	1288, 2254, 3478, 3643, 3745
BstYI	RGATCY	5 cut(s)	1288, 2254, 3478, 3643, 3745
BstZ17I	GTATAC	3 cut(s)	965, 1940, 4028
Bsu15I	ATCGAT	1 cut(s)	2183
Bsu36I	CCTNAGG	1 cut(s)	1818
BsuI	GTATCC	1 cut(s)	3644
BsuTUI	ATCGAT	1 cut(s)	2183
BtgI	CCRYGG	1 cut(s)	3490
BtsI	GCAGTG	3 cut(s)	2353, 2826, 3052
BveI	ACCTGC	1 cut(s)	3143
CaiI	CAGNNNCTG	2 cut(s)	2540, 3341
CciI	TCATGA	2 cut(s)	1063, 3141
CfoI	GCGC	2 cut(s)	2619, 4085
Cfr13I	GGNCC	5 cut(s)	2030, 2849, 2995, 3469, 3791
Cfr9I	CCCGGG	1 cut(s)	385
ClaI	ATCGAT	1 cut(s)	2183
CseI	GACGC	2 cut(s)	1258, 3327
CsiI	ACCWGGT	1 cut(s)	3769
DpnI	GATC	9 cut(s)	1290, 2256, 2390, 2515, 2953, 3480, 3645, 3686, 3747
DpnII	GATC	9 cut(s)	1288, 2254, 2388, 2513, 2951, 3478, 3643, 3684, 3745
DraIII	CACNNNGTG	1 cut(s)	371
Eam1104I	CTCTTC	5 cut(s)	11, 18, 2157, 2730, 2913
EarI	CTCTTC	5 cut(s)	11, 18, 2157, 2730, 2913
Ecl136II	GAGCTC	2 cut(s)	751, 2167
Eco130I	CCWWGG	3 cut(s)	1328, 2001, 3490
Eco147I	AGGCCT	2 cut(s)	324, 1701
Eco24I	GRGCYC	3 cut(s)	753, 2169, 3909
Eco31I	GGTCTC	2 cut(s)	143, 1818
Eco32I	GATATC	1 cut(s)	639
Eco47I	GGWCC	2 cut(s)	2030, 2849
Eco53kI	GAGCTC	2 cut(s)	751, 2167
Eco57I	CTGAAG	7 cut(s)	239, 995, 1826, 2046, 3402, 3875, 3993
Eco81I	CCTNAGG	1 cut(s)	1818
Eco88I	CYCGRG	1 cut(s)	385
Eco91I	GGTNACC	1 cut(s)	1274
EcoICRI	GAGCTC	2 cut(s)	751, 2167
EcoO65I	GGTNACC	1 cut(s)	1274
EcoRI	GAATTC	2 cut(s)	72, 1410
EcoRII	CCWGG	6 cut(s)	1542, 1606, 1975, 3147, 3769, 3793
EcoRV	GATATC	1 cut(s)	639
EcoT14I	CCWWGG	3 cut(s)	1328, 2001, 3490
EcoT22I	ATGCAT	4 cut(s)	250, 412, 1462, 3382
EcoT38I	GRGCYC	3 cut(s)	753, 2169, 3909
ErhI	CCWWGG	3 cut(s)	1328, 2001, 3490
FalI	AAGNNNNNCTT	2 cut(s)	2633, 2665
FaqI	GGGAC	4 cut(s)	160, 1813, 2808, 3873
FauI	CCCGC	1 cut(s)	3006
FauNDI	CATATG	1 cut(s)	2385
FblI	GTMKAC	4 cut(s)	964, 1939, 3171, 4027
FriOI	GRGCYC	3 cut(s)	753, 2169, 3909
FspBI	CTAG	8 cut(s)	836, 1335, 2306, 2511, 2714, 3191, 3621, 3725
GlaI	GCGC	2 cut(s)	2618, 4084
GsaI	CCCAGC	2 cut(s)	2174, 3668
GsuI	CTGGAG	1 cut(s)	3627
HaeII	RGCGCY	1 cut(s)	2620
HapII	CCGG	4 cut(s)	33, 386, 1113, 3472
HgaI	GACGC	2 cut(s)	1258, 3327
HhaI	GCGC	2 cut(s)	2619, 4085
Hin6I	GCGC	2 cut(s)	2617, 4083
HinP1I	GCGC	2 cut(s)	2617, 4083
HincII	GTYRAC	1 cut(s)	2044
HindII	GTYRAC	1 cut(s)	2044
HindIII	AAGCTT	2 cut(s)	152, 4088
HpaII	CCGG	4 cut(s)	33, 386, 1113, 3472
HphI	GGTGA	9 cut(s)	95, 100, 176, 426, 593, 1313, 1448, 2957, 3914
Hpy99I	CGWCG	2 cut(s)	81, 1252
HpyCH4III	ACNGT	9 cut(s)	190, 264, 295, 369, 650, 1280, 2588, 2987, 3187
HpyCH4IV	ACGT	6 cut(s)	285, 1054, 2151, 3400, 3825, 4024
HpySE526I	ACGT	6 cut(s)	285, 1054, 2151, 3400, 3825, 4024
HspAI	GCGC	2 cut(s)	2617, 4083
Kzo9I	GATC	9 cut(s)	1288, 2254, 2388, 2513, 2951, 3478, 3643, 3684, 3745
LguI	GCTCTTC	2 cut(s)	11, 2157
MabI	ACCWGGT	1 cut(s)	3769
MaeI	CTAG	8 cut(s)	836, 1335, 2306, 2511, 2714, 3191, 3621, 3725
MaeII	ACGT	6 cut(s)	285, 1054, 2151, 3400, 3825, 4024
MalI	GATC	9 cut(s)	1290, 2256, 2390, 2515, 2953, 3480, 3645, 3686, 3747
MboI	GATC	9 cut(s)	1288, 2254, 2388, 2513, 2951, 3478, 3643, 3684, 3745
MfeI	CAATTG	4 cut(s)	501, 1395, 1947, 3669
MflI	RGATCY	5 cut(s)	1288, 2254, 3478, 3643, 3745
MhlI	GDGCHC	8 cut(s)	188, 596, 753, 1564, 2169, 3160, 3426, 3909
MlyI	GAGTC	2 cut(s)	50, 90
MmeI	TCCRAC	2 cut(s)	1517, 2008
Mph1103I	ATGCAT	4 cut(s)	250, 412, 1462, 3382
MroXI	GAANNNNTTC	1 cut(s)	788
MslI	CAYNNNNRTG	5 cut(s)	191, 783, 2275, 3623, 3867
MspA1I	CMGCKG	3 cut(s)	1684, 1805, 2015
MspCI	CTTAAG	3 cut(s)	824, 1033, 1703
MspI	CCGG	4 cut(s)	33, 386, 1113, 3472
MunI	CAATTG	4 cut(s)	501, 1395, 1947, 3669
Mva1269I	GAATGC	1 cut(s)	1408
MvaI	CCWGG	6 cut(s)	1544, 1608, 1977, 3149, 3771, 3795
MvnI	CGCG	2 cut(s)	1907, 4083
NciI	CCSGG	5 cut(s)	34, 386, 387, 1114, 3472
NcoI	CCATGG	1 cut(s)	3490
NdeI	CATATG	1 cut(s)	2385
NdeII	GATC	9 cut(s)	1288, 2254, 2388, 2513, 2951, 3478, 3643, 3684, 3745
NlaIV	GGNNCC	9 cut(s)	258, 311, 1541, 2032, 2337, 2997, 3315, 3408, 3775
NmuCI	GTSAC	5 cut(s)	650, 668, 2071, 2279, 3193
NsiI	ATGCAT	4 cut(s)	250, 412, 1462, 3382
NspI	RCATGY	6 cut(s)	252, 950, 1904, 3358, 3384, 3462
NspV	TTCGAA	1 cut(s)	1216
OliI	CACNNNNGTG	1 cut(s)	3623
PaeI	GCATGC	3 cut(s)	252, 1904, 3462
PagI	TCATGA	2 cut(s)	1063, 3141
PaqCI	CACCTGC	1 cut(s)	3143
PceI	AGGCCT	2 cut(s)	324, 1701
PciI	ACATGT	2 cut(s)	946, 3354
PciSI	GCTCTTC	2 cut(s)	11, 2157
PctI	GAATGC	1 cut(s)	1408
PdmI	GAANNNNTTC	1 cut(s)	788
PfeI	GAWTC	8 cut(s)	341, 789, 970, 1226, 1955, 2560, 2773, 2881
PflFI	GACNNNGTC	1 cut(s)	2285
PleI	GAGTC	2 cut(s)	49, 89
PpsI	GAGTC	2 cut(s)	49, 89
Ppu21I	YACGTR	2 cut(s)	286, 3401
PscI	ACATGT	2 cut(s)	946, 3354
PshBI	ATTAAT	2 cut(s)	705, 2934
Psp124BI	GAGCTC	2 cut(s)	753, 2169
Psp6I	CCWGG	6 cut(s)	1542, 1606, 1975, 3147, 3769, 3793
PspEI	GGTNACC	1 cut(s)	1274
PspFI	CCCAGC	2 cut(s)	2170, 3664
PspGI	CCWGG	6 cut(s)	1542, 1606, 1975, 3147, 3769, 3793
PspN4I	GGNNCC	9 cut(s)	258, 311, 1541, 2032, 2337, 2997, 3315, 3408, 3775
PspPI	GGNCC	5 cut(s)	2030, 2849, 2995, 3469, 3791
PsrI	GAACNNNNNNTAC	4 cut(s)	598, 630, 948, 980
PstI	CTGCAG	3 cut(s)	1006, 2536, 3370
PstNI	CAGNNNCTG	2 cut(s)	2540, 3341
PsuI	RGATCY	5 cut(s)	1288, 2254, 3478, 3643, 3745
PsyI	GACNNNGTC	1 cut(s)	2285
PvuII	CAGCTG	3 cut(s)	1684, 1805, 2015
RseI	CAYNNNNRTG	5 cut(s)	191, 783, 2275, 3623, 3867
SacI	GAGCTC	2 cut(s)	753, 2169
SapI	GCTCTTC	2 cut(s)	11, 2157
Sau3AI	GATC	9 cut(s)	1288, 2254, 2388, 2513, 2951, 3478, 3643, 3684, 3745
Sau96I	GGNCC	5 cut(s)	2030, 2849, 2995, 3469, 3791
ScaI	AGTACT	1 cut(s)	834
SchI	GAGTC	2 cut(s)	50, 90
SduI	GDGCHC	8 cut(s)	188, 596, 753, 1564, 2169, 3160, 3426, 3909
SexAI	ACCWGGT	1 cut(s)	3769
SfcI	CTRYAG	3 cut(s)	1002, 2532, 3366
SfuI	TTCGAA	1 cut(s)	1216
SinI	GGWCC	2 cut(s)	2030, 2849
SmaI	CCCGGG	1 cut(s)	387
SmiMI	CAYNNNNRTG	5 cut(s)	191, 783, 2275, 3623, 3867
SphI	GCATGC	3 cut(s)	252, 1904, 3462
SseBI	AGGCCT	2 cut(s)	324, 1701
SsiI	CCGC	5 cut(s)	1905, 2490, 2499, 2999, 4056
SspI	AATATT	3 cut(s)	1914, 2958, 3268
SspMI	CTAG	8 cut(s)	836, 1335, 2306, 2511, 2714, 3191, 3621, 3725
SstI	GAGCTC	2 cut(s)	753, 2169
StuI	AGGCCT	2 cut(s)	324, 1701
StyI	CCWWGG	3 cut(s)	1328, 2001, 3490
TaaI	ACNGT	9 cut(s)	190, 264, 295, 369, 650, 1280, 2588, 2987, 3187
TaiI	ACGT	6 cut(s)	288, 1057, 2154, 3403, 3828, 4027
TaqI	TCGA	9 cut(s)	44, 79, 481, 1216, 1835, 2183, 2563, 3516, 3687
TatI	WGTACW	3 cut(s)	832, 1157, 2987
TauI	GCSGC	2 cut(s)	1907, 2502
TfiI	GAWTC	8 cut(s)	341, 789, 970, 1226, 1955, 2560, 2773, 2881
TseFI	GTSAC	5 cut(s)	650, 668, 2071, 2279, 3193
Tsp45I	GTSAC	5 cut(s)	650, 668, 2071, 2279, 3193
TspGWI	ACGGA	4 cut(s)	391, 737, 2609, 4045
TspMI	CCCGGG	1 cut(s)	385
Tth111I	GACNNNGTC	1 cut(s)	2285
Vha464I	CTTAAG	3 cut(s)	824, 1033, 1703
VneI	GTGCAC	1 cut(s)	184
VpaK11BI	GGWCC	2 cut(s)	2030, 2849
VspI	ATTAAT	2 cut(s)	705, 2934
XceI	RCATGY	6 cut(s)	252, 950, 1904, 3358, 3384, 3462
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2627
XmaI	CCCGGG	1 cut(s)	385
XmiI	GTMKAC	4 cut(s)	964, 1939, 3171, 4027
XmnI	GAANNNNTTC	1 cut(s)	788
XspI	CTAG	8 cut(s)	836, 1335, 2306, 2511, 2714, 3191, 3621, 3725
ZrmI	AGTACT	1 cut(s)	834
Zsp2I	ATGCAT	4 cut(s)	250, 412, 1462, 3382

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.