Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0005756

Physical Location & Seq

Genomic Coordinates Forward (+)

24377852 .. 24389447

Gene Structure 11596 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1177200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4053 bp

ATGGGATCGTTGAAGAAGGAAGAGGAGGAGCTGGAACAATCGGGGGAGGAGTCAAAAGAGGCCATACTATATGTCAATGGAGTTCGTAGAGTTTTGCCTGATGGGTTGGCCCACTTGACCCTTCTCGAGTACCTCAGAGATTTAGGTTTGACAGGGACAAAGCTTGGATGTGGTGAAGGTGGTTGCGGTGCTTGCACTGTTATGGTTTCTCATTACAACAAAGAACTGAAGAAAAGTTTCCACTATGCTGTCAATGCATGCTTGGCTCCTTTGTACTCTGTAGAAGGGATGCAGGTAATTACAGTGGAAGGACTAGGGAGCCACAAAAAGGGCTTGCACCCAATTCAAGAATCGTTGGCACGATCTCATGGTTCACAATGTGGATTTTGCACCCCCGGTTTTATTATGTCCATCTACGCCTTGTTAAGATCAAGTCAGAAACCCCCTAGTGAGGGGGAGATTGAAGAAAGTCTTGCAGGAAATTTGTGTAGATGCACAGGTTACAGACCAATTGTTGATGCTTTTCGTGTCTTTGCCAAAACGAATGATGCACCATACGTTAATACATCTCCACTAAGCTCTGAAGGAGGTGAGTTTGTGTGCCCTTCGACTGGAAAGCCCTGTTCATGTGGATTGAAAAGTGAAAGTTCATGCACCACTCATGAAAGTGGTATTCATGATGAGAGGTATGCACCTGTCTCGTACAGTGAAATTGATGGAAGCACGTACACAGACAAGGAGTTTATTTTTCCTCCTGAGCTATTATTGAGAAAATGGACTTATTTAAGTTTGACTGGTTTTAGTGGATTAAAGTGGTTCCGACCCTTGAGGCTTAAACAAGTGCTAGAATTGAAGGAAAAATATCCAGATGCAAAATTATTGGTAGGTAATACTGAGGTGGGAATTGAAACGAGGTTGAAGAAACTTCAATATCAGGTTTTGATTTCCGTTACGCATGTACCTGAACTCAGTAAATTGACTGTGAAGGATGATGGCATAGAGATAGGTTCAGCAGTAAGACTTTCTGAACTACTTAAAGTTTTAAGAATGGTCATAAAAGAACGCGCAGCTCATGAAACTTCTGCTTGTAAGGCCTTCGTTGAACAATTAAAATGGTTTGCTGGGGTGCAAATAAGAAATGTTGCGTGTGTTGGTGGAAATATCTGTACAGCCAGTCCAATATCAGACTTAAACCCTCTTTGGATGGCCTCTAGAGCGAAATTTCAGATCATTGATTCCAAAGGGAATATTAGAACGATGCTGGCAGAAAACTTTTTCCTGGGTTACCGAAAGGTGGATCTGGCAAGTGGTGAAATTTTACTCTCTGTATTTTTACCTTGGACTAGATCTTTCGAGTATGTGAAAGAGTATAAGCAAGCTCACAGAAGGGATGATGATATTGCAATTGTGAATGCTGGGATTCGTGTTCATCTTGAGGACAGAGGTGGCTGGGTTGTGTCTGATGCGTGCATTGTTTATGGTGGAGTTGCTCCAGTCTCTCTTGCTGCAACAAGAACGAAAGATTTTCTCATTGGAAAGAGATGGAACCAGGAGATGTTGCAGGGTGCCTTGAAAGTCCTGCAAAAAGATGTATTGCTCAAGGATGACGCTCCTGGTGGGATGGTGGAGTTTCGTAAATCTTTGACTGTAAGCTTTTTCTTCAAATTTTTTCTTTGGGTTTCTCATCAAATGGAAGGGAAGCAATGCATTAAAGTGAGTGTACCGTTATCTCATCTTTCTGCTGTACAATCATTTCACCGGCCACCTGTTATAGGAGCTCAGGACTACGAAGTTATAAAACGTGGGACAGCAGTTGGGTCTCCCGAAGTTCATTTATCAGCAAGACTTCAGGTTACAGGAGAGGCGGAATATGCCGATGACACACCACTGCCTCCTAATGGTTTACACGCTGCTTTAATACTCAGCAGAAAGCCTCATGCTCGTATACTTTCAATCGATGATTCTGGAGCCAAGTTATCATCTGGGTTTGCAGGCATATATTTAGCAAAGGACGTTCCAGCTGATAATAATGTTGGACCAATTATTGAAGACGAGGAACTATTTGCTTCGGAATTTGTCACTTGTGTAGGTCAGGTTATAGGAGTGGTAGTTGCTGATACACATGAAAATGCAAAACTAGCAGCAAGGAAGGTTCATGTTGAGTATGAAGAGCTCCCAGCCATCGTATCAATACAGGATGCCATCGAAGCCAAAAGTTTCCATCCTGATACGAAGAGGTGTTTAAGGAAAGGGGATGTGGACCTCTGCTTTCAATCAAGTCAATGTGACAACGTCATAGAGGGGGAAGTTCGAGTAGGCGGACAGGAGCACTTCTATTTGGAGCCAAATAGCAGTGTGGTATGGACAATGGATGGTGGCAATGAAGTTCATATGGTCTCATCCACTCAGGCCCCTCAGAAGCACCAGAAATACGTTGCTCATGTTCTTGGTCTTTCAATGTCGAAAGTTGTTTGTAAAACTAAGCGCATTGGCGGGGGTTTTGGCGGCAAGGAGACTAGGTCAGCTGTCGTTGCTGCTGCAGCCGCTGTTCCATCATATCTGTTAAATCGTCCAGTTAAAATTACACTAGACCGTGATACAGACATGATGATAACTGGCCAACGCCATAGCTTTCTTGGAAAGTACAAGGTTGGATTTACAAATGAGGATTTGTCTCTTCCCATACTTGAACGTGCTATGTTTCAGTCAGATAACGTCTATGAGATTCCAAATGTGAGGATTGTGGGAAGGGTTTGCTTCACTAACATTACAAGTAACACTGCTTTTCGAGGATTTGGTGGTCCTCAAGGTATGATTATTGTGGAAAATTGGATTCAGAGGATTGCTGTAGAACTCAAAAAAAGCCCAGAGGAGATAAAAGAAATTAATTTTCAAGGTGAAGGATCAATATTGCATTATGGTCAACAACTTAAACACTGCACATTGGGTCCTGTGTGGAATCAACTGAAGTTATCTTGCGACTTTTCGAAGGCTCGCTACGAAGTTGACCAATTTAATATTCAAAATCGGTGGCGGAAGCGTGGTATTGCCATGGTTCCAACAAAATTTGGCATAGCCTTTACGTTAAAGCTTATGAACCAGGCAGGTGCTCTCGTTCATGTCTACACAGATGGAACTGTTTTAGTCACACATGGAGGTGTTGAGATGGGGCAAGGTTTACACACTAAAGTTGCTCAGGTCGCTGCTTCCGCCTTCAGTATCCCTCTCAGTTCTGTCTTTATTTCAGAGACAAGCACCGACAAGGTTCCTAATGCATCGGCAACAGCAGCTTCTGCGAGTTCTGACATGTATGGAGCTGCAGTTCTGGATGCATGTGAGAAAATTAAGGCACGTATGAAGCCTATTGCTTCACAGCAAAATTTCAGTTCTTTTGCCGAGCTGGCAAGTGCATGCTATGTTGAACGGATAGATCTTTCTGCTCACGGGTTTTACATTACACCGGACATTGACTTTGATTGGACAACTGGTAAAGGGAACCCATTCAGCTACTTCACCTATGGGGCTGCTTTTGCTGAGGTTGAAATTGATACATTGACTGGAGATTTTCACACTAGGGCGGCTAACATATTCTTGGATCTTGGTTACTCTCTCAACCCCGCAATTGATGTCGGGCAGATTGAAGGAGCATTTGTACAAGGTTTGGGATGGGTAGCTCTAGAAGAACTAAAGCGGGGCGATCCTGCTCATAAATGGATCTCGCCTGGTTGCCTCTACACATCTGGGCCTGGAAGTTACAAGATTCCTTCCATAAATGATGTCCCCTTCAAATTCAGCGTTTCACTTCTGAAGGGCCATCCAAATGTGAAGGCCATCCACTCATCTAAAGCTGTTGGTGAGCCACCATTTTTCTTGGCATCAGCCGTCTTTTTCGCCATAAAGGACGCCATTATAGCTGCAAGAGCAGAAGTAGGGTCTAATGACTGGTTTCCACTTGATAATCCCGCGACACCTGAACGGATACGTATGGCTTGCTTAGACGAAATTATAGGACCATTTGTAAGCACAGATTTCCGCGCCAAGCTTAGCGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0000302 GO:0001101 GO:0003674 GO:0003824 GO:0004854 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006139 GO:0006144 GO:0006145 GO:0006725 GO:0006801 GO:0006807 GO:0006950 GO:0006979 GO:0008150 GO:0008152 GO:0009056 GO:0009112 GO:0009115 GO:0009414 GO:0009415 GO:0009628 GO:0009987 GO:0010035 GO:0016491 GO:0016725 GO:0016726 GO:0016903 GO:0019439 GO:0034641 GO:0036094 GO:0042221 GO:0042554 GO:0043167 GO:0043168 GO:0043546 GO:0044237 GO:0044238 GO:0044248 GO:0044270 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046110 GO:0046113 GO:0046483 GO:0046700 GO:0048037 GO:0050660 GO:0050662 GO:0050896 GO:0051536 GO:0051537 GO:0051540 GO:0055086 GO:0055114 GO:0071704 GO:0072521 GO:0072523 GO:0072593 GO:0097159 GO:1901265 GO:1901360 GO:1901361 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00230 ko00232 ko00983 ko01100 ko01110 ko01120 ko04146 map00230 map00232 map00983 map01100 map01110 map01120 map04146

Pfam Domains

Protein Families

Ald_Xan_dh_C Ald_Xan_dh_C2 CO_deh_flav_C FAD_binding_5 Fer2 Fer2_2

Protein Analysis

1351

Amino Acids

148.03

Weight (kDa)

6.62

Isoelectric Point (pI)

38.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Fer2	PF00111	27 - 90	7.1e-09	2Fe-2S iron-sulfur cluster binding domain
Fer2_2	PF01799	101 - 175	4.2e-32	[2Fe-2S] binding domain
FAD_binding_5	PF00941	270 - 447	7.1e-49	FAD binding domain in molybdopterin dehydrogenase
CO_deh_flav_C	PF03450	456 - 560	6e-31	CO dehydrogenase flavoprotein C-terminal domain
Ald_Xan_dh_C	PF01315	619 - 726	1.2e-28	Aldehyde oxidase and xanthine dehydrogenase, a/b hammerhead domain
MoCoBD_1	PF02738	745 - 964	3.3e-70	Molybdopterin cofactor-binding domain
MoCoBD_2	PF20256	990 - 1261	4.7e-89	Molybdopterin cofactor-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0005756)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G34890 AT4G34900 AT4G34900 AT4G34900
fragaria_vesca	FvH4_3g29000 FvH4_3g29000 FvH4_3g29000 FvH4_3g29000
malus_domestica	MD03G1177200.v1.1 MD11G1161000.v1.1 MD11G1197900.v1.1
prunus_persica	Prupe.4G245700_v2.0.a1 Prupe.4G245700_v2.0.a1
pyrus_communis	pycom11g13410 pycom11g17110 pycom11g17120
rosa_chinensis	RchiOBHm_Chr5g0051661
rosa_laevigata	RLG00000034866
rosa_multiflora	Rmu_sc0000068.1_g000026 Rmu_sc0014882.1_g000001 Rmu_sc0018333.1_g000002
rosa_roxburghii	Rroxscaffold_1G00027170
rosa_rugosa	Rorug05G0277900
rosa_samantha	Rh5AG349700 Rh5BG361400 Rh5CG385900 Rh5DG376200
rosa_wichuraiana	Rw5G033070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1796
AarI	CACCTGC	1 cut(s)	3095
Acc36I	ACCTGC	2 cut(s)	283, 3095
AccB1I	GGYRCC	1 cut(s)	1565
AccI	GTMKAC	2 cut(s)	1945, 3123
AccII	CGCG	3 cut(s)	1065, 3965, 4035
AclWI	GGATC	6 cut(s)	13, 1305, 2911, 3603, 3692, 3722
AcoI	YGGCCR	2 cut(s)	1760, 2617
AcsI	RAATTY	8 cut(s)	481, 1220, 1314, 1664, 2072, 3065, 3379, 3788
AcuI	CTGAAG	6 cut(s)	248, 603, 1832, 2987, 3199, 3827
AcyI	GRCGYC	1 cut(s)	3903
AdeI	CACNNNGTG	1 cut(s)	380
AfiI	CCNNNNNNNGG	7 cut(s)	328, 451, 452, 611, 1294, 1772, 1898
AflIII	ACRYGT	1 cut(s)	3306
AjnI	CCWGG	6 cut(s)	1278, 1548, 1612, 3099, 3721, 3745
AjuI	GAANNNNNNNTTGG	2 cut(s)	3003, 3035
AloI	GAACNNNNNNTCC	2 cut(s)	3306, 3338
Alw21I	GWGCWC	4 cut(s)	1780, 2175, 2331, 3112
Alw26I	GTCTC	7 cut(s)	703, 1501, 1824, 2401, 2507, 2679, 3242
AlwI	GGATC	6 cut(s)	13, 1305, 2911, 3603, 3692, 3722
AlwNI	CAGNNNCTG	2 cut(s)	2546, 3293
Ama87I	CYCGRG	1 cut(s)	125
ApoI	RAATTY	8 cut(s)	481, 1220, 1314, 1664, 2072, 3065, 3379, 3788
ArsI	GACNNNNNNTTYG	2 cut(s)	3455, 3487
AseI	ATTAAT	1 cut(s)	2886
Asp700I	GAANNNNTTC	2 cut(s)	236, 3500
AspLEI	GCGC	3 cut(s)	1067, 2487, 4037
AspS9I	GGNCC	9 cut(s)	109, 2036, 2260, 2410, 2801, 2948, 3743, 3811, 4010
AsuC2I	CCSGG	1 cut(s)	396
AsuHPI	GGTGA	7 cut(s)	185, 602, 1322, 1748, 2909, 3505, 3866
AsuII	TTCGAA	1 cut(s)	2987
AvaI	CYCGRG	1 cut(s)	125
AvaII	GGWCC	5 cut(s)	2036, 2260, 2801, 2948, 4010
BaeGI	GKGCMC	1 cut(s)	605
BaeI	ACNNNNGTAYC	2 cut(s)	942, 975
BalI	TGGCCA	1 cut(s)	2619
BanI	GGYRCC	1 cut(s)	1565
BanII	GRGCYC	2 cut(s)	1780, 2175
BarI	GAAGNNNNNNTAC	2 cut(s)	2981, 3013
BbsI	GAAGAC	1 cut(s)	2055
Bbv12I	GWGCWC	4 cut(s)	1780, 2175, 2331, 3112
BbvCI	CCTCAGC	1 cut(s)	3534
BceAI	ACGGC	1 cut(s)	3866
BcgI	CGANNNNNNTGC	2 cut(s)	681, 715
BciT130I	CCWGG	6 cut(s)	1280, 1550, 1614, 3101, 3723, 3747
BciVI	GTATCC	2 cut(s)	3230, 3972
BcnI	CCSGG	1 cut(s)	396
BcoDI	GTCTC	7 cut(s)	703, 1501, 1824, 2401, 2507, 2679, 3242
BfmI	CTRYAG	4 cut(s)	279, 2538, 2847, 3318
BfuAI	ACCTGC	2 cut(s)	283, 3095
BfuI	GTATCC	2 cut(s)	3230, 3972
BglI	GCCNNNNNGGC	1 cut(s)	3401
BglII	AGATCT	2 cut(s)	1346, 3430
BlpI	GCTNAGC	1 cut(s)	4043
Bme1390I	CCNGG	7 cut(s)	396, 1280, 1550, 1614, 3101, 3723, 3747
Bme18I	GGWCC	5 cut(s)	2036, 2260, 2801, 2948, 4010
BmeT110I	CYCGRG	1 cut(s)	125
BmgT120I	GGNCC	9 cut(s)	109, 2036, 2260, 2410, 2801, 2948, 3743, 3811, 4010
BmrFI	CCNGG	7 cut(s)	396, 1280, 1550, 1614, 3101, 3723, 3747
BpiI	GAAGAC	1 cut(s)	2055
BpmI	CTGGAG	3 cut(s)	1476, 1986, 3579
Bpu10I	CCTNAGC	4 cut(s)	756, 1779, 3195, 3534
Bpu1102I	GCTNAGC	1 cut(s)	4043
Bpu14I	TTCGAA	1 cut(s)	2987
BpuEI	CTTGAG	4 cut(s)	847, 1454, 1583, 2790
BpuMI	CCSGG	1 cut(s)	396
Bsa29I	ATCGAT	1 cut(s)	1956
BsaAI	YACGTR	3 cut(s)	726, 3353, 3983
BsaHI	GRCGYC	1 cut(s)	3903
BsaI	GGTCTC	2 cut(s)	1824, 2401
BsaJI	CCNNGG	4 cut(s)	394, 1279, 1337, 3051
BsaWI	WCCGGW	1 cut(s)	3460
BsaXI	ACNNNNNCTCC	8 cut(s)	2094, 2124, 2504, 2534, 3306, 3336, 3636, 3666
Bsc4I	CCNNNNNNNGG	7 cut(s)	328, 451, 452, 611, 1294, 1772, 1898
Bse118I	RCCGGY	1 cut(s)	1758
Bse1I	ACTGG	9 cut(s)	616, 799, 1173, 1493, 2573, 2620, 3490, 3562, 3947
Bse3DI	GCAATG	2 cut(s)	1709, 2386
BseBI	CCWGG	6 cut(s)	1280, 1550, 1614, 3101, 3723, 3747
BseCI	ATCGAT	1 cut(s)	1956
BseDI	CCNNGG	4 cut(s)	394, 1279, 1337, 3051
BseLI	CCNNNNNNNGG	7 cut(s)	328, 451, 452, 611, 1294, 1772, 1898
BseMI	GCAATG	2 cut(s)	1709, 2386
BseNI	ACTGG	9 cut(s)	616, 799, 1173, 1493, 2573, 2620, 3490, 3562, 3947
BseRI	GAGGAG	4 cut(s)	38, 41, 62, 2885
BseSI	GKGCMC	1 cut(s)	605
BseYI	CCCAGC	4 cut(s)	1121, 1415, 1449, 2176
BsgI	GTGCAG	1 cut(s)	2923
Bsh1236I	CGCG	3 cut(s)	1065, 3965, 4035
BshNI	GGYRCC	1 cut(s)	1565
BshVI	ATCGAT	1 cut(s)	1956
BsiHKAI	GWGCWC	4 cut(s)	1780, 2175, 2331, 3112
BsiHKCI	CYCGRG	1 cut(s)	125
BsiSI	CCGG	3 cut(s)	396, 1759, 3461
BslFI	GGGAC	3 cut(s)	169, 1819, 3764
BslI	CCNNNNNNNGG	7 cut(s)	328, 451, 452, 611, 1294, 1772, 1898
BsmAI	GTCTC	7 cut(s)	703, 1501, 1824, 2401, 2507, 2679, 3242
BsmFI	GGGAC	3 cut(s)	169, 1819, 3764
BsmI	GAATGC	1 cut(s)	1417
Bso31I	GGTCTC	2 cut(s)	1824, 2401
BsoBI	CYCGRG	1 cut(s)	125
Bsp119I	TTCGAA	1 cut(s)	2987
Bsp1286I	GDGCHC	5 cut(s)	605, 1780, 2175, 2331, 3112
Bsp1407I	TGTACA	3 cut(s)	1166, 1744, 3652
Bsp1720I	GCTNAGC	1 cut(s)	4043
Bsp19I	CCATGG	1 cut(s)	3051
BspDI	ATCGAT	1 cut(s)	1956
BspFNI	CGCG	3 cut(s)	1065, 3965, 4035
BspHI	TCATGA	3 cut(s)	661, 676, 1072
BspMAI	CTGCAG	2 cut(s)	2542, 3322
BspMI	ACCTGC	2 cut(s)	283, 3095
BspPI	GGATC	6 cut(s)	13, 1305, 2911, 3603, 3692, 3722
BspQI	GCTCTTC	1 cut(s)	2163
BspT104I	TTCGAA	1 cut(s)	2987
BspT107I	GGYRCC	1 cut(s)	1565
BspTNI	GGTCTC	2 cut(s)	1824, 2401
BsrDI	GCAATG	2 cut(s)	1709, 2386
BsrFI	RCCGGY	1 cut(s)	1758
BsrGI	TGTACA	3 cut(s)	1166, 1744, 3652
BsrI	ACTGG	9 cut(s)	616, 799, 1173, 1493, 2573, 2620, 3490, 3562, 3947
BssAI	RCCGGY	1 cut(s)	1758
BssECI	CCNNGG	4 cut(s)	394, 1279, 1337, 3051
BssNAI	GTATAC	1 cut(s)	1946
BssNI	GRCGYC	1 cut(s)	3903
BssT1I	CCWWGG	2 cut(s)	1337, 3051
Bst1107I	GTATAC	1 cut(s)	1946
Bst2UI	CCWGG	6 cut(s)	1280, 1550, 1614, 3101, 3723, 3747
Bst4CI	ACNGT	8 cut(s)	199, 304, 707, 982, 1648, 1725, 2594, 3139
Bst6I	CTCTTC	4 cut(s)	15, 2163, 2228, 2682
BstACI	GRCGYC	1 cut(s)	3903
BstAPI	GCANNNNNTGC	1 cut(s)	3293
BstAUI	TGTACA	3 cut(s)	1166, 1744, 3652
BstBAI	YACGTR	3 cut(s)	726, 3353, 3983
BstBI	TTCGAA	1 cut(s)	2987
BstDSI	CCRYGG	1 cut(s)	3051
BstEII	GGTNACC	1 cut(s)	1283
BstENI	CCTNNNNNAGG	1 cut(s)	1770
BstFNI	CGCG	3 cut(s)	1065, 3965, 4035
BstHHI	GCGC	3 cut(s)	1067, 2487, 4037
BstMAI	GTCTC	7 cut(s)	703, 1501, 1824, 2401, 2507, 2679, 3242
BstNI	CCWGG	6 cut(s)	1280, 1550, 1614, 3101, 3723, 3747
BstNSI	RCATGY	5 cut(s)	261, 959, 3310, 3336, 3414
BstPI	GGTNACC	1 cut(s)	1283
BstSCI	CCNGG	7 cut(s)	394, 1278, 1548, 1612, 3099, 3721, 3745
BstSFI	CTRYAG	4 cut(s)	279, 2538, 2847, 3318
BstSLI	GKGCMC	1 cut(s)	605
BstSNI	TACGTA	1 cut(s)	3983
BstUI	CGCG	3 cut(s)	1065, 3965, 4035
BstV2I	GAAGAC	1 cut(s)	2055
BstX2I	RGATCY	5 cut(s)	1297, 1346, 3430, 3595, 3714
BstYI	RGATCY	5 cut(s)	1297, 1346, 3430, 3595, 3714
BstZ17I	GTATAC	1 cut(s)	1946
Bsu15I	ATCGAT	1 cut(s)	1956
BsuI	GTATCC	2 cut(s)	3230, 3972
BsuTUI	ATCGAT	1 cut(s)	1956
BtgI	CCRYGG	1 cut(s)	3051
BtsI	GCAGTG	4 cut(s)	1886, 2359, 2778, 2935
BtsIMutI	CAGTG	7 cut(s)	195, 309, 712, 1886, 2359, 2778, 2935
BveI	ACCTGC	2 cut(s)	283, 3095
CaiI	CAGNNNCTG	2 cut(s)	2546, 3293
CciI	TCATGA	3 cut(s)	661, 676, 1072
CfoI	GCGC	3 cut(s)	1067, 2487, 4037
Cfr10I	RCCGGY	1 cut(s)	1758
Cfr13I	GGNCC	9 cut(s)	109, 2036, 2260, 2410, 2801, 2948, 3743, 3811, 4010
ClaI	ATCGAT	1 cut(s)	1956
CseI	GACGC	2 cut(s)	1616, 3911
CspCI	CAANNNNNGTGG	2 cut(s)	3011, 3046
DraIII	CACNNNGTG	1 cut(s)	380
EaeI	YGGCCR	2 cut(s)	1760, 2617
Eam1104I	CTCTTC	4 cut(s)	15, 2163, 2228, 2682
EarI	CTCTTC	4 cut(s)	15, 2163, 2228, 2682
EciI	GGCGGA	4 cut(s)	1880, 2334, 3049, 3199
Ecl136II	GAGCTC	2 cut(s)	1778, 2173
Eco105I	TACGTA	1 cut(s)	3983
Eco130I	CCWWGG	2 cut(s)	1337, 3051
Eco147I	AGGCCT	1 cut(s)	1094
Eco24I	GRGCYC	2 cut(s)	1780, 2175
Eco31I	GGTCTC	2 cut(s)	1824, 2401
Eco47I	GGWCC	5 cut(s)	2036, 2260, 2801, 2948, 4010
Eco53kI	GAGCTC	2 cut(s)	1778, 2173
Eco57I	CTGAAG	6 cut(s)	248, 603, 1832, 2987, 3199, 3827
Eco88I	CYCGRG	1 cut(s)	125
Eco91I	GGTNACC	1 cut(s)	1283
EcoICRI	GAGCTC	2 cut(s)	1778, 2173
EcoNI	CCTNNNNNAGG	1 cut(s)	1770
EcoO109I	RGGNCCY	2 cut(s)	2410, 2948
EcoO65I	GGTNACC	1 cut(s)	1283
EcoRII	CCWGG	6 cut(s)	1278, 1548, 1612, 3099, 3721, 3745
EcoT14I	CCWWGG	2 cut(s)	1337, 3051
EcoT22I	ATGCAT	4 cut(s)	259, 1709, 3277, 3334
EcoT38I	GRGCYC	2 cut(s)	1780, 2175
ErhI	CCWWGG	2 cut(s)	1337, 3051
FalI	AAGNNNNNCTT	2 cut(s)	456, 488
FaqI	GGGAC	3 cut(s)	169, 1819, 3764
FauI	CCCGC	4 cut(s)	2486, 3625, 3684, 3970
FauNDI	CATATG	1 cut(s)	2391
FblI	GTMKAC	2 cut(s)	1945, 3123
FriOI	GRGCYC	2 cut(s)	1780, 2175
GlaI	GCGC	3 cut(s)	1066, 2486, 4036
GsaI	CCCAGC	4 cut(s)	1125, 1419, 1453, 2180
GsuI	CTGGAG	3 cut(s)	1476, 1986, 3579
HapII	CCGG	3 cut(s)	396, 1759, 3461
HgaI	GACGC	2 cut(s)	1616, 3911
HhaI	GCGC	3 cut(s)	1067, 2487, 4037
Hin1I	GRCGYC	1 cut(s)	3903
Hin6I	GCGC	3 cut(s)	1065, 2485, 4035
HinP1I	GCGC	3 cut(s)	1065, 2485, 4035
HincII	GTYRAC	2 cut(s)	2924, 3007
HindII	GTYRAC	2 cut(s)	2924, 3007
HindIII	AAGCTT	4 cut(s)	161, 1651, 3089, 4040
HinfI	GANTC	9 cut(s)	50, 350, 1235, 1420, 1961, 2725, 2833, 2959, 3760
HpaII	CCGG	3 cut(s)	396, 1759, 3461
HphI	GGTGA	7 cut(s)	185, 602, 1322, 1748, 2909, 3505, 3866
HpyCH4III	ACNGT	8 cut(s)	199, 304, 707, 982, 1648, 1725, 2594, 3139
Hsp92I	GRCGYC	1 cut(s)	3903
HspAI	GCGC	3 cut(s)	1065, 2485, 4035
LguI	GCTCTTC	1 cut(s)	2163
MfeI	CAATTG	3 cut(s)	510, 1404, 3621
MflI	RGATCY	5 cut(s)	1297, 1346, 3430, 3595, 3714
MhlI	GDGCHC	5 cut(s)	605, 1780, 2175, 2331, 3112
MlsI	TGGCCA	1 cut(s)	2619
MluNI	TGGCCA	1 cut(s)	2619
MlyI	GAGTC	1 cut(s)	59
MmeI	TCCRAC	4 cut(s)	844, 2014, 2632, 3083
Mox20I	TGGCCA	1 cut(s)	2619
Mph1103I	ATGCAT	4 cut(s)	259, 1709, 3277, 3334
MroXI	GAANNNNTTC	2 cut(s)	236, 3500
MscI	TGGCCA	1 cut(s)	2619
MslI	CAYNNNNRTG	6 cut(s)	200, 666, 1712, 2127, 3156, 3819
Msp20I	TGGCCA	1 cut(s)	2619
MspA1I	CMGCKG	3 cut(s)	2021, 2525, 2546
MspI	CCGG	3 cut(s)	396, 1759, 3461
MspR9I	CCNGG	7 cut(s)	396, 1280, 1550, 1614, 3101, 3723, 3747
MunI	CAATTG	3 cut(s)	510, 1404, 3621
Mva1269I	GAATGC	1 cut(s)	1417
MvaI	CCWGG	6 cut(s)	1280, 1550, 1614, 3101, 3723, 3747
MvnI	CGCG	3 cut(s)	1065, 3965, 4035
NciI	CCSGG	1 cut(s)	396
NcoI	CCATGG	1 cut(s)	3051
NdeI	CATATG	1 cut(s)	2391
NmeAIII	GCCGAG	1 cut(s)	3421
NmuCI	GTSAC	3 cut(s)	2077, 2285, 3145
NsiI	ATGCAT	4 cut(s)	259, 1709, 3277, 3334
NspI	RCATGY	5 cut(s)	261, 959, 3310, 3336, 3414
NspV	TTCGAA	1 cut(s)	2987
PaeI	GCATGC	2 cut(s)	261, 3414
PaeR7I	CTCGAG	1 cut(s)	125
PagI	TCATGA	3 cut(s)	661, 676, 1072
PaqCI	CACCTGC	1 cut(s)	3095
PceI	AGGCCT	1 cut(s)	1094
PciI	ACATGT	1 cut(s)	3306
PciSI	GCTCTTC	1 cut(s)	2163
PctI	GAATGC	1 cut(s)	1417
PdmI	GAANNNNTTC	2 cut(s)	236, 3500
PfeI	GAWTC	8 cut(s)	350, 1235, 1420, 1961, 2725, 2833, 2959, 3760
PflFI	GACNNNGTC	2 cut(s)	2291, 2518
PleI	GAGTC	1 cut(s)	58
PpsI	GAGTC	1 cut(s)	58
Ppu21I	YACGTR	3 cut(s)	726, 3353, 3983
PpuMI	RGGWCCY	1 cut(s)	2948
PscI	ACATGT	1 cut(s)	3306
PshBI	ATTAAT	1 cut(s)	2886
PsiI	TTATAA	1 cut(s)	1796
Psp124BI	GAGCTC	2 cut(s)	1780, 2175
Psp5II	RGGWCCY	1 cut(s)	2948
Psp6I	CCWGG	6 cut(s)	1278, 1548, 1612, 3099, 3721, 3745
PspEI	GGTNACC	1 cut(s)	1283
PspFI	CCCAGC	4 cut(s)	1121, 1415, 1449, 2176
PspGI	CCWGG	6 cut(s)	1278, 1548, 1612, 3099, 3721, 3745
PspPI	GGNCC	9 cut(s)	109, 2036, 2260, 2410, 2801, 2948, 3743, 3811, 4010
PspPPI	RGGWCCY	1 cut(s)	2948
PstI	CTGCAG	2 cut(s)	2542, 3322
PstNI	CAGNNNCTG	2 cut(s)	2546, 3293
PsuI	RGATCY	5 cut(s)	1297, 1346, 3430, 3595, 3714
PsyI	GACNNNGTC	2 cut(s)	2291, 2518
PvuII	CAGCTG	2 cut(s)	2021, 2525
RseI	CAYNNNNRTG	6 cut(s)	200, 666, 1712, 2127, 3156, 3819
SacI	GAGCTC	2 cut(s)	1780, 2175
SapI	GCTCTTC	1 cut(s)	2163
Sau96I	GGNCC	9 cut(s)	109, 2036, 2260, 2410, 2801, 2948, 3743, 3811, 4010
SchI	GAGTC	1 cut(s)	59
ScrFI	CCNGG	7 cut(s)	396, 1280, 1550, 1614, 3101, 3723, 3747
SduI	GDGCHC	5 cut(s)	605, 1780, 2175, 2331, 3112
SfcI	CTRYAG	4 cut(s)	279, 2538, 2847, 3318
Sfr274I	CTCGAG	1 cut(s)	125
SfuI	TTCGAA	1 cut(s)	2987
SinI	GGWCC	5 cut(s)	2036, 2260, 2801, 2948, 4010
SlaI	CTCGAG	1 cut(s)	125
SmiMI	CAYNNNNRTG	6 cut(s)	200, 666, 1712, 2127, 3156, 3819
SmlI	CTYRAG	5 cut(s)	125, 826, 1433, 1598, 2805
SmoI	CTYRAG	5 cut(s)	125, 826, 1433, 1598, 2805
SnaBI	TACGTA	1 cut(s)	3983
SphI	GCATGC	2 cut(s)	261, 3414
SseBI	AGGCCT	1 cut(s)	1094
SspI	AATATT	3 cut(s)	1249, 2910, 3019
SstI	GAGCTC	2 cut(s)	1780, 2175
StuI	AGGCCT	1 cut(s)	1094
StyD4I	CCNGG	7 cut(s)	394, 1278, 1548, 1612, 3099, 3721, 3745
StyI	CCWWGG	2 cut(s)	1337, 3051
TaaI	ACNGT	8 cut(s)	199, 304, 707, 982, 1648, 1725, 2594, 3139
TaqI	TCGA	9 cut(s)	126, 608, 1353, 1956, 2205, 2311, 2462, 2788, 2987
TatI	WGTACW	5 cut(s)	273, 1166, 1744, 2643, 3652
TauI	GCSGC	3 cut(s)	2508, 2546, 3581
TfiI	GAWTC	8 cut(s)	350, 1235, 1420, 1961, 2725, 2833, 2959, 3760
TscAI	CASTG	7 cut(s)	202, 309, 712, 1893, 2359, 2785, 2942
TseFI	GTSAC	3 cut(s)	2077, 2285, 3145
Tsp45I	GTSAC	3 cut(s)	2077, 2285, 3145
TspGWI	ACGGA	3 cut(s)	937, 3439, 3991
TspRI	CASTG	7 cut(s)	202, 309, 712, 1893, 2359, 2785, 2942
Tth111I	GACNNNGTC	2 cut(s)	2291, 2518
VpaK11BI	GGWCC	5 cut(s)	2036, 2260, 2801, 2948, 4010
VspI	ATTAAT	1 cut(s)	2886
XagI	CCTNNNNNAGG	1 cut(s)	1770
XapI	RAATTY	8 cut(s)	481, 1220, 1314, 1664, 2072, 3065, 3379, 3788
XbaI	TCTAGA	2 cut(s)	1211, 3676
XceI	RCATGY	5 cut(s)	261, 959, 3310, 3336, 3414
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2633
XhoI	CTCGAG	1 cut(s)	125
XmiI	GTMKAC	2 cut(s)	1945, 3123
XmnI	GAANNNNTTC	2 cut(s)	236, 3500
Zsp2I	ATGCAT	4 cut(s)	259, 1709, 3277, 3334

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.