FvH4_4g00210

SH3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0004649
Physical Location & Seq
Forward (+)
228902 .. 245790
16889 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g00210.t1

Sequence Viewer

Length: 3693 bp
ATGCTGCCTGGGCATCTAAAACAAGTGAAATCGAAGCAGGAAGGAAGACTCGACTCTCTTCCTTTCTCTCTCCCTGATTGTTTAGCCATGGCGGACTCGTCAGGGACGACCCTGATGGATCTGATAACGGCCGACCCGTCCACGGTGTCAGCAACGACGTCGTCTTCGTCATCGGCGCAGTCGTCGGCTCCTCCTCCTCCGTATGCGGCGGCTTCTCGGGGCACCAGCCCGGGCTCGGCTCTGGGAAAGCCTGCCGTGGAGAAGAGGTCGAAGCGGGCGGCACTGATGCAGATCCAGAACGACACCATCTCCGCCGCCAAAGCTGCTTTGAATCCCGTGCGCACCAACATCATCATGGGGCCCCAGAAGAACCGCCACAAGCAGAAAAAGCCTGTTTCATACGCTCAACTTGCGAGGAGCATTCACGAGCTGGCTGCTTCTTCCGATCAGAAAAGTTCCCAGAAGCAGTTGGTGAATCACGTCTTTCCCAAGCTTGCCGTCTACAATTCTGTTGACCCCTCCGTCGCCCCTTCTCTTCTCATGCTCAATCAGCAGTGTGAGGATAAAAGCGTTCTACGGTATGTCTATTATTACTTGGCCAGGATTTTATCTGACACTGGTGCTCAAGGTGTAACCACGGGCGGTGGGATCCCAACTCCAAACTGGGATGCTCTAGCTGACATTGATGCTATCGGAGGGGTCACTCGAGCTGATGTTGTACCAAGAATAGTAAATCAGCTCACTATAGAGGCTAAAAATGCCGATCCAGAATTTCATGCACGAAGACTGCAAGCTTTGAAGGCTCTTACATATGCCCCATCAACAAATTCTGAAATATTGTCCCAATTGTATGAAATTGTTTTTGGCATTCTTGATAAGGTTGCTGATGGCCCACAAAAACGCAAGAAAGGTGTGTTTGGGACTAAAGGTGGCGATAAAGAGTTCATCATCCGAAGTAACTTGCAATATGGTGCCCTAAGTGCTCTTAGAAGACTTCCATTGGATCCAGGAAACCCAGCTTTTTTGTACCGTGCTGTTCAAGGGGTTTCGTTTGCTGATCCTGTTGCTGTAAGGCATTCTTTGGAAATTCTGTTTGAGCTAGCCACAAAAGACCCTTATGCAGTTGCAATGGGCTTAGGGAAACACGCAGAGCCTGGAGGGGCTTTACAGGATGTTCTCCATTTGCATGATGTCCTTGCTAGAGTTGCTCTGGCTAGGTTGTGCTATACAATTTCAAGAGCACGAGCATTGGATGAGAGGCCTGATATTAGATCTCAGTTCAACTCTGTACTCTATCAACTCCTCCTGGATCCCAGCGAAAGAGTTTGTTTTGAGGCAATCTTATGTATATTGGGAAAACAGGATAACTCAGAAAGGACTGACGATCGAGCTGCCGGATGGTACCGATTAACTAGAGAGATTCTCAAGTTGCCTGAAGCACCTTCTGTGAAGGATTCCTCCAAAGATAAAGCTCAAAAGACAAGGCGCCCTCAACCCCTCATTAAACTTGTAATGAGAAGATTGGAGAGTTCTTTCCGAAGTTTTTCTAGGCCAGTACTCCATGCAGCATCAAGAGTTGTGCAGGAGATGGGAAAGAGTAGGGCTGCTGCATTTGCTCTAGGCATACAGGATATCGACGAAACAGTGCATGTTAACACATTTTCTGAGACTGTTGATTCCCGCGAGATAGATAGTAGTGAAGCCTCACATCCTGAGAGTATCCGAAGAACGTCCTCCTTATCTACTGGGGTAGGTGGCAAGGATACAATTGCTAGTTTGTTGGCTTCATTAATGGAAGTTGTGCGGACAACCGTGGCATGTGAGTGTGTTTATGTTCGAGCTATGGTTATTAAGGCATTGATCTGGATGCAAAGTCCACATGATTCGTTTGATCAACTAGAATCTATAATTGCATCAGAGCTTTCTGATCCAGCTTGGCCAGCCACGCTCCTGAATGATATTTTACTCACTTTGCATGCTCGGTTTAAGGCAACTCCAGATATGGCCGTCACTCTTCTTGAAATTGCAAGGATCTTTGCAACTAAAGCTCCGGGGAAGATTGATGCTGATGTACTACAACTACTGTGGAAGACTTGCCTTGTTGGAGCTGGTCCTGATGGGAAACATACTGCATTAGAAGCGGTTACCATAGTTCTTGATTTACCACCACCACAACCTGGGTCAATGTTGGGGATTACATCAGTTGACAGGGTATCTGCTTCTGATCCAAAGGCAGCTCTGGCTTTGCAGAGATTAGTACAAGCTGCAGTATGGTTTCTTGGAGAAAATGCAAACTATGCTGCTTCTGAATATGCTTGGGAATCCACAACCCCTCCTGGTACCGCATTGATGATGCTAGATGCAGATAAGATGGTTGCTGCAGCCAGTTCCCGTAACCCTACTCTAGCTGGTGCATTGACTAGGCTACAGAGGTGTGCATTCAGTGGCAGCTGGGAGGTTCGGATCATTGCTGCTCAAGCTCTCACAACTATGGCAATAAGATCTGGTGAGCCTTTTAGGCTGCAAATATACGAATTTCTACACACTATAGCTCAGGGCGGAGTGCAGTCTCAGTTTTCTGAGATGCATCCCAGTAATGGAGAAGACCAGGGAGCTAGTGGAACAGGCCTCGGAGTTCTAATCAGTCCCATGATAGAGGTTCTTGATGAAATGTATAGGGCGCAAGATGATTTGATCAAGGAAATGCGCAACCATGATAATGTGAATAAGGAATGGACAGATGAAGAGCTTAAGAAACTCTACGAAACCCATGAAAGGTTGCTTGATCTTGTTTCTCTGTTTTGTTATGTTCCAAGAGCAAAATATCTGCCTTTGGGGCCAATAAGTGCAAAACTTATCGACATCTATCGCACCAGGCACAATATTAGTGCATCAACTGGTTTGAGTGATCCAGCAGTTGCTACAGGAATTTCTGATCTTATGTATGAATCTAAACCAGCAGCAGTAGAGTCCGATATGCTTGATGATGACCTTGTAAATGCTTGGGCAGCAAACCTTGGTGATGATGGACTTTTAGGAAATAATGCACCGGCATTGAGCAGGGTTAATGAATTTCTTGCTGGTGCTGGAACTGATGCTCCTGATGTTGATGAAGAAAACATAATCTCTAGGCCATCAGTTAGTTATGATGATATGTGGGCAAAGACGCTTTTAGAGACTTCAGAACTGGAGGAAGAGGATGCGAGGTCATCCGGGTCATCTTCCCCTGAATCAACAGGCTCGGTTGAAACTTCCATATCATCCCATTTTGGGGGAATGAATTACCCTTCACTTTTCAGTTCACGGCCGGAGAGGTCTGGAGGAAGCAGATATAGCAATCCATCTATGGGGGGTCCCTCATTCAGTGAGGGTTTAGGATCTCCAATTAGAGAAGATCCTCCGCCATACTCGTCACCTGCCACGCAAAGATTTGAGTCGTTTGAGAATCCCCTAGCTGGATCCCAGAGTTTTGGATCCCAAGATGACGAACGTGTTTCTTCTGGAAATCCTCAACATGGAACAGCACTTTATGACTTCACTGCTGGTGGAGATGATGAGTTAAATTTAACATCCGGAGAGGAGGTTGATATTGAGTATGAAGTGGATGGCTGGTTTTATGTAAAGAAGAAACGCCCTGGGAGAGATGGGAAAATGGCTGGTCTGGTCCCAGTCCTCTATGTTAGTCAATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SH3_1 SH3_9

Protein Analysis

1231

Amino Acids

133.3

Weight (kDa)

5.46

Isoelectric Point (pI)

48.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SH3_1 PF00018 1174 - 1215 1e-05 SH3 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 3424
AasI GACNNNNNNGTC 1 cut(s) 521
AatII GACGTC 1 cut(s) 161
Acc16I TGCGCA 2 cut(s) 341, 2708
Acc36I ACCTGC 1 cut(s) 3424
Acc65I GGTACC 2 cut(s) 1401, 2339
AccB1I GGYRCC 5 cut(s) 221, 971, 1401, 1485, 2339
AccB7I CCANNNNNTGG 1 cut(s) 2177
AccI GTMKAC 1 cut(s) 501
AccII CGCG 1 cut(s) 1683
AccIII TCCGGA 1 cut(s) 3572
AcoI YGGCCR 5 cut(s) 129, 597, 1937, 2004, 3305
AcsI RAATTY 7 cut(s) 770, 826, 1086, 2534, 2928, 3071, 3562
AcuI CTGAAG 2 cut(s) 1455, 3165
AcyI GRCGYC 2 cut(s) 158, 1486
AfaI GTAC 8 cut(s) 720, 1028, 1290, 1403, 1557, 2073, 2259, 2341
AflII CTTAAG 1 cut(s) 2750
AflIII ACRYGT 1 cut(s) 3490
AgsI TTSAA 7 cut(s) 331, 799, 1040, 1236, 1282, 2021, 3248
AjiI CACGTC 1 cut(s) 481
AjuI GAANNNNNNNTTGG 2 cut(s) 846, 878
AloI GAACNNNNNNTCC 2 cut(s) 3082, 3114
Alw21I GWGCWC 3 cut(s) 625, 985, 1243
Alw26I GTCTC 3 cut(s) 1661, 2574, 3170
AlwNI CAGNNNCTG 1 cut(s) 1154
Ama87I CYCGRG 3 cut(s) 216, 229, 705
Aor13HI TCCGGA 1 cut(s) 3572
ApaI GGGCCC 1 cut(s) 363
ApoI RAATTY 7 cut(s) 770, 826, 1086, 2534, 2928, 3071, 3562
ArsI GACNNNNNNTTYG 2 cut(s) 148, 180
AseI ATTAAT 1 cut(s) 1790
Asp700I GAANNNNTTC 1 cut(s) 1543
Asp718I GGTACC 2 cut(s) 1401, 2339
AspLEI GCGC 5 cut(s) 178, 342, 1488, 2683, 2709
AspS9I GGNCC 7 cut(s) 359, 360, 890, 2111, 2837, 3353, 3664
AsuC2I CCSGG 4 cut(s) 230, 231, 2052, 3214
AsuHPI GGTGA 4 cut(s) 484, 2519, 3032, 3405
AsuNHI GCTAGC 1 cut(s) 1099
AvaI CYCGRG 3 cut(s) 216, 229, 705
AvaII GGWCC 3 cut(s) 2111, 3353, 3664
BaeGI GKGCMC 3 cut(s) 224, 363, 976
BalI TGGCCA 2 cut(s) 599, 1939
BamHI GGATCC 5 cut(s) 648, 1003, 1309, 3458, 3473
BanI GGYRCC 5 cut(s) 221, 971, 1401, 1485, 2339
BanII GRGCYC 2 cut(s) 236, 363
BauI CACGAG 2 cut(s) 425, 1242
BbsI GAAGAC 6 cut(s) 52, 156, 790, 997, 2096, 2610
Bbv12I GWGCWC 3 cut(s) 625, 985, 1243
BceAI ACGGC 5 cut(s) 144, 239, 482, 1991, 3320
BcgI CGANNNNNNTGC 6 cut(s) 141, 175, 416, 450, 1373, 1407
BciVI GTATCC 2 cut(s) 1730, 1756
BclI TGATCA 2 cut(s) 1891, 2694
BcnI CCSGG 4 cut(s) 230, 231, 2052, 3214
BcoDI GTCTC 3 cut(s) 1661, 2574, 3170
BfmI CTRYAG 6 cut(s) 744, 2265, 2379, 2426, 2547, 2922
BfoI RGCGCY 1 cut(s) 1489
BfrI CTTAAG 1 cut(s) 2750
BfuAI ACCTGC 1 cut(s) 3424
BfuI GTATCC 2 cut(s) 1730, 1756
BglI GCCNNNNNGGC 2 cut(s) 2518, 2836
BglII AGATCT 2 cut(s) 1271, 2501
BmcAI AGTACT 1 cut(s) 1557
Bme18I GGWCC 3 cut(s) 2111, 3353, 3664
BmeT110I CYCGRG 3 cut(s) 216, 229, 705
BmgBI CACGTC 1 cut(s) 481
BmgT120I GGNCC 7 cut(s) 359, 360, 890, 2111, 2837, 3353, 3664
BmrI ACTGGG 4 cut(s) 673, 1755, 2586, 3662
BmtI GCTAGC 1 cut(s) 1103
BmuI ACTGGG 4 cut(s) 673, 1755, 2586, 3662
BpiI GAAGAC 6 cut(s) 52, 156, 790, 997, 2096, 2610
BplI GAGNNNNNCTC 2 cut(s) 1407, 1439
BpmI CTGGAG 4 cut(s) 1176, 1980, 3209, 3339
Bpu10I CCTNAGC 2 cut(s) 1135, 2553
BpuEI CTTGAG 3 cut(s) 609, 1409, 2460
BpuMI CCSGG 4 cut(s) 230, 231, 2052, 3214
BsaBI GATNNNNATC 1 cut(s) 290
BsaHI GRCGYC 2 cut(s) 158, 1486
BsaWI WCCGGW 1 cut(s) 3572
Bse118I RCCGGY 1 cut(s) 3049
Bse1I ACTGG 9 cut(s) 622, 668, 1553, 1750, 2385, 2592, 2902, 3192, 3668
Bse3DI GCAATG 2 cut(s) 1134, 2466
Bse8I GATNNNNATC 1 cut(s) 290
BseAI TCCGGA 1 cut(s) 3572
BseJI GATNNNNATC 1 cut(s) 290
BseMI GCAATG 2 cut(s) 1134, 2466
BseMII CTCAG 7 cut(s) 1289, 1383, 1656, 1704, 2567, 2571, 2585
BseNI ACTGG 9 cut(s) 622, 668, 1553, 1750, 2385, 2592, 2902, 3192, 3668
BseRI GAGGAG 6 cut(s) 180, 183, 186, 430, 1292, 3593
BseSI GKGCMC 3 cut(s) 224, 363, 976
BseX3I CGGCCG 2 cut(s) 129, 3305
BseYI CCCAGC 3 cut(s) 1015, 1313, 2451
BsgI GTGCAG 2 cut(s) 1601, 2585
Bsh1236I CGCG 1 cut(s) 1683
Bsh1285I CGRYCG 3 cut(s) 132, 1387, 3308
BshNI GGYRCC 5 cut(s) 221, 971, 1401, 1485, 2339
BsiEI CGRYCG 3 cut(s) 132, 1387, 3308
BsiHKAI GWGCWC 3 cut(s) 625, 985, 1243
BsiHKCI CYCGRG 3 cut(s) 216, 229, 705
BsiSI CCGG 7 cut(s) 230, 1395, 2051, 3050, 3213, 3308, 3573
BslFI GGGAC 6 cut(s) 118, 826, 934, 2631, 3339, 3650
BsmAI GTCTC 3 cut(s) 1661, 2574, 3170
BsmFI GGGAC 6 cut(s) 118, 826, 934, 2631, 3339, 3650
BsmI GAATGC 4 cut(s) 420, 867, 1075, 2438
BsoBI CYCGRG 3 cut(s) 216, 229, 705
Bsp120I GGGCCC 1 cut(s) 359
Bsp1286I GDGCHC 7 cut(s) 224, 236, 363, 625, 976, 985, 1243
Bsp13I TCCGGA 1 cut(s) 3572
Bsp19I CCATGG 1 cut(s) 87
BspCNI CTCAG 7 cut(s) 1288, 1382, 1657, 1705, 2566, 2572, 2584
BspEI TCCGGA 1 cut(s) 3572
BspFNI CGCG 1 cut(s) 1683
BspMAI CTGCAG 2 cut(s) 2269, 2383
BspMI ACCTGC 1 cut(s) 3424
BspOI GCTAGC 1 cut(s) 1103
BspQI GCTCTTC 1 cut(s) 2739
BspT107I GGYRCC 5 cut(s) 221, 971, 1401, 1485, 2339
BspTI CTTAAG 1 cut(s) 2750
BsrDI GCAATG 2 cut(s) 1134, 2466
BsrFI RCCGGY 1 cut(s) 3049
BsrI ACTGG 9 cut(s) 622, 668, 1553, 1750, 2385, 2592, 2902, 3192, 3668
BssAI RCCGGY 1 cut(s) 3049
BssNI GRCGYC 2 cut(s) 158, 1486
BssSI CACGAG 2 cut(s) 425, 1242
BssT1I CCWWGG 2 cut(s) 87, 3016
Bst2BI CACGAG 2 cut(s) 425, 1242
Bst4CI ACNGT 7 cut(s) 145, 579, 1031, 1645, 1672, 1813, 2085
Bst6I CTCTTC 6 cut(s) 63, 257, 540, 2019, 2739, 3189
BstACI GRCGYC 2 cut(s) 158, 1486
BstAFI CTTAAG 1 cut(s) 2750
BstAPI GCANNNNNTGC 1 cut(s) 2297
BstC8I GCNNGC 8 cut(s) 252, 276, 432, 495, 792, 1101, 1941, 1977
BstDSI CCRYGG 5 cut(s) 87, 141, 255, 636, 1812
BstEII GGTNACC 1 cut(s) 2143
BstFNI CGCG 1 cut(s) 1683
BstH2I RGCGCY 1 cut(s) 1489
BstHHI GCGC 5 cut(s) 178, 342, 1488, 2683, 2709
BstMAI GTCTC 3 cut(s) 1661, 2574, 3170
BstMCI CGRYCG 3 cut(s) 132, 1387, 3308
BstNSI RCATGY 3 cut(s) 1652, 1821, 1979
BstPI GGTNACC 1 cut(s) 2143
BstSFI CTRYAG 6 cut(s) 744, 2265, 2379, 2426, 2547, 2922
BstSLI GKGCMC 3 cut(s) 224, 363, 976
BstUI CGCG 1 cut(s) 1683
BstV2I GAAGAC 6 cut(s) 52, 156, 790, 997, 2096, 2610
BstXI CCANNNNNNTGG 1 cut(s) 3470
BstZI CGGCCG 2 cut(s) 129, 3305
BsuI GTATCC 2 cut(s) 1730, 1756
BtgI CCRYGG 5 cut(s) 87, 141, 255, 636, 1812
BtrI CACGTC 1 cut(s) 481
BtsI GCAGTG 2 cut(s) 560, 3537
BtsIMutI CAGTG 7 cut(s) 281, 560, 615, 1650, 2449, 3370, 3537
BveI ACCTGC 1 cut(s) 3424
Cac8I GCNNGC 8 cut(s) 252, 276, 432, 495, 792, 1101, 1941, 1977
CaiI CAGNNNCTG 1 cut(s) 1154
CfoI GCGC 5 cut(s) 178, 342, 1488, 2683, 2709
Cfr10I RCCGGY 1 cut(s) 3049
Cfr13I GGNCC 7 cut(s) 359, 360, 890, 2111, 2837, 3353, 3664
Cfr9I CCCGGG 1 cut(s) 229
CseI GACGC 1 cut(s) 3175
Csp6I GTAC 8 cut(s) 719, 1027, 1289, 1402, 1556, 2072, 2258, 2340
CspCI CAANNNNNGTGG 2 cut(s) 2066, 2101
CviQI GTAC 8 cut(s) 719, 1027, 1289, 1402, 1556, 2072, 2258, 2340
DinI GGCGCC 1 cut(s) 1487
DrdI GACNNNNNNGTC 1 cut(s) 521
DseDI GACNNNNNNGTC 1 cut(s) 521
EaeI YGGCCR 5 cut(s) 129, 597, 1937, 2004, 3305
EagI CGGCCG 2 cut(s) 129, 3305
Eam1104I CTCTTC 6 cut(s) 63, 257, 540, 2019, 2739, 3189
EarI CTCTTC 6 cut(s) 63, 257, 540, 2019, 2739, 3189
EciI GGCGGA 4 cut(s) 107, 301, 2574, 3390
EclXI CGGCCG 2 cut(s) 129, 3305
Eco130I CCWWGG 2 cut(s) 87, 3016
Eco147I AGGCCT 2 cut(s) 1261, 2628
Eco24I GRGCYC 2 cut(s) 236, 363
Eco32I GATATC 1 cut(s) 1633
Eco47I GGWCC 3 cut(s) 2111, 3353, 3664
Eco52I CGGCCG 2 cut(s) 129, 3305
Eco57I CTGAAG 2 cut(s) 1455, 3165
Eco88I CYCGRG 3 cut(s) 216, 229, 705
Eco91I GGTNACC 1 cut(s) 2143
EcoO109I RGGNCCY 3 cut(s) 359, 360, 3353
EcoO65I GGTNACC 1 cut(s) 2143
EcoRV GATATC 1 cut(s) 1633
EcoT14I CCWWGG 2 cut(s) 87, 3016
EcoT22I ATGCAT 1 cut(s) 2589
EcoT38I GRGCYC 2 cut(s) 236, 363
EgeI GGCGCC 1 cut(s) 1487
EheI GGCGCC 1 cut(s) 1487
ErhI CCWWGG 2 cut(s) 87, 3016
FalI AAGNNNNNCTT 2 cut(s) 1063, 1095
FaqI GGGAC 6 cut(s) 118, 826, 934, 2631, 3339, 3650
FauI CCCGC 2 cut(s) 267, 1688
FauNDI CATATG 1 cut(s) 811
FbaI TGATCA 2 cut(s) 1891, 2694
FblI GTMKAC 1 cut(s) 501
FriOI GRGCYC 2 cut(s) 236, 363
FspAI RTGCGCAY 1 cut(s) 341
FspI TGCGCA 2 cut(s) 341, 2708
GlaI GCGC 5 cut(s) 177, 341, 1487, 2682, 2708
GsaI CCCAGC 3 cut(s) 1019, 1317, 2455
GsuI CTGGAG 4 cut(s) 1176, 1980, 3209, 3339
HaeII RGCGCY 1 cut(s) 1489
HapII CCGG 7 cut(s) 230, 1395, 2051, 3050, 3213, 3308, 3573
HgaI GACGC 1 cut(s) 3175
HhaI GCGC 5 cut(s) 178, 342, 1488, 2683, 2709
Hin1I GRCGYC 2 cut(s) 158, 1486
Hin6I GCGC 5 cut(s) 176, 340, 1486, 2681, 2707
HinP1I GCGC 5 cut(s) 176, 340, 1486, 2681, 2707
HincII GTYRAC 3 cut(s) 514, 1654, 2206
HindII GTYRAC 3 cut(s) 514, 1654, 2206
HindIII AAGCTT 2 cut(s) 491, 792
HpaI GTTAAC 1 cut(s) 1654
HpaII CCGG 7 cut(s) 230, 1395, 2051, 3050, 3213, 3308, 3573
HphI GGTGA 4 cut(s) 484, 2519, 3032, 3405
Hpy166II GTNNAC 7 cut(s) 141, 502, 514, 1654, 1877, 2206, 3302
Hpy8I GTNNAC 7 cut(s) 141, 502, 514, 1654, 1877, 2206, 3302
Hpy99I CGWCG 5 cut(s) 160, 163, 187, 527, 1640
HpyAV CCTTC 6 cut(s) 35, 540, 793, 1444, 1452, 3297
HpyCH4III ACNGT 7 cut(s) 145, 579, 1031, 1645, 1672, 1813, 2085
HpyCH4IV ACGT 4 cut(s) 158, 480, 1730, 3490
HpySE526I ACGT 4 cut(s) 158, 480, 1730, 3490
Hsp92I GRCGYC 2 cut(s) 158, 1486
HspAI GCGC 5 cut(s) 176, 340, 1486, 2681, 2707
KasI GGCGCC 1 cut(s) 1485
KflI GGGWCCC 1 cut(s) 3353
Kpn2I TCCGGA 1 cut(s) 3572
KpnI GGTACC 2 cut(s) 1405, 2343
Ksp22I TGATCA 2 cut(s) 1891, 2694
KspAI GTTAAC 1 cut(s) 1654
LguI GCTCTTC 1 cut(s) 2739
LmnI GCTCC 7 cut(s) 193, 417, 1953, 2053, 2105, 2612, 3103
MaeII ACGT 4 cut(s) 158, 480, 1730, 3490
MaeIII GTNAC 7 cut(s) 631, 700, 956, 2008, 2143, 2393, 3411
MfeI CAATTG 2 cut(s) 845, 1767
MhlI GDGCHC 7 cut(s) 224, 236, 363, 625, 976, 985, 1243
MlsI TGGCCA 2 cut(s) 599, 1939
MluNI TGGCCA 2 cut(s) 599, 1939
Mly113I GGCGCC 1 cut(s) 1486
MlyI GAGTC 5 cut(s) 42, 47, 89, 2978, 3443
MmeI TCCRAC 1 cut(s) 2083
Mox20I TGGCCA 2 cut(s) 599, 1939
Mph1103I ATGCAT 1 cut(s) 2589
MroI TCCGGA 1 cut(s) 3572
MroXI GAANNNNTTC 1 cut(s) 1543
MscI TGGCCA 2 cut(s) 599, 1939
MslI CAYNNNNRTG 5 cut(s) 353, 1185, 1822, 2489, 2718
Msp20I TGGCCA 2 cut(s) 599, 1939
MspA1I CMGCKG 1 cut(s) 2451
MspCI CTTAAG 1 cut(s) 2750
MspI CCGG 7 cut(s) 230, 1395, 2051, 3050, 3213, 3308, 3573
MunI CAATTG 2 cut(s) 845, 1767
Mva1269I GAATGC 4 cut(s) 420, 867, 1075, 2438
MvnI CGCG 1 cut(s) 1683
NarI GGCGCC 1 cut(s) 1486
NciI CCSGG 4 cut(s) 230, 231, 2052, 3214
NcoI CCATGG 1 cut(s) 87
NdeI CATATG 1 cut(s) 811
NheI GCTAGC 1 cut(s) 1099
NmeAIII GCCGAG 1 cut(s) 215
NmuCI GTSAC 3 cut(s) 700, 2008, 3411
NsbI TGCGCA 2 cut(s) 341, 2708
NsiI ATGCAT 1 cut(s) 2589
NspI RCATGY 3 cut(s) 1652, 1821, 1979
PaeI GCATGC 1 cut(s) 1979
PaeR7I CTCGAG 1 cut(s) 705
PaqCI CACCTGC 1 cut(s) 3424
PasI CCCWGGG 1 cut(s) 3635
PceI AGGCCT 2 cut(s) 1261, 2628
PciSI GCTCTTC 1 cut(s) 2739
PcsI WCGNNNNNNNCGW 4 cut(s) 104, 134, 164, 179
PctI GAATGC 4 cut(s) 420, 867, 1075, 2438
PdmI GAANNNNTTC 1 cut(s) 1543
PflFI GACNNNGTC 1 cut(s) 160
PflMI CCANNNNNTGG 1 cut(s) 2177
PfoI TCCNGGA 2 cut(s) 1006, 1305
Ple19I CGATCG 1 cut(s) 1387
PleI GAGTC 5 cut(s) 42, 47, 89, 2977, 3442
PluTI GGCGCC 1 cut(s) 1489
PpsI GAGTC 5 cut(s) 42, 47, 89, 2977, 3442
PpuMI RGGWCCY 1 cut(s) 3353
PshBI ATTAAT 1 cut(s) 1790
Psp5II RGGWCCY 1 cut(s) 3353
PspEI GGTNACC 1 cut(s) 2143
PspFI CCCAGC 3 cut(s) 1015, 1313, 2451
PspOMI GGGCCC 1 cut(s) 359
PspPI GGNCC 7 cut(s) 359, 360, 890, 2111, 2837, 3353, 3664
PspPPI RGGWCCY 1 cut(s) 3353
PspXI VCTCGAGB 1 cut(s) 705
PstI CTGCAG 2 cut(s) 2269, 2383
PstNI CAGNNNCTG 1 cut(s) 1154
PsyI GACNNNGTC 1 cut(s) 160
PvuI CGATCG 1 cut(s) 1387
PvuII CAGCTG 1 cut(s) 2451
RsaI GTAC 8 cut(s) 720, 1028, 1290, 1403, 1557, 2073, 2259, 2341
RsaNI GTAC 8 cut(s) 719, 1027, 1289, 1402, 1556, 2072, 2258, 2340
RseI CAYNNNNRTG 5 cut(s) 353, 1185, 1822, 2489, 2718
SapI GCTCTTC 1 cut(s) 2739
Sau96I GGNCC 7 cut(s) 359, 360, 890, 2111, 2837, 3353, 3664
ScaI AGTACT 1 cut(s) 1557
SchI GAGTC 5 cut(s) 42, 47, 89, 2978, 3443
SduI GDGCHC 7 cut(s) 224, 236, 363, 625, 976, 985, 1243
SfcI CTRYAG 6 cut(s) 744, 2265, 2379, 2426, 2547, 2922
SfoI GGCGCC 1 cut(s) 1487
Sfr274I CTCGAG 1 cut(s) 705
SinI GGWCC 3 cut(s) 2111, 3353, 3664
SlaI CTCGAG 1 cut(s) 705
SmaI CCCGGG 1 cut(s) 231
SmiMI CAYNNNNRTG 5 cut(s) 353, 1185, 1822, 2489, 2718
SmlI CTYRAG 5 cut(s) 624, 705, 1424, 2475, 2750
SmoI CTYRAG 5 cut(s) 624, 705, 1424, 2475, 2750
SphI GCATGC 1 cut(s) 1979
SrfI GCCCGGGC 1 cut(s) 231
SseBI AGGCCT 2 cut(s) 1261, 2628
SspDI GGCGCC 1 cut(s) 1485
SspI AATATT 2 cut(s) 837, 2884
StuI AGGCCT 2 cut(s) 1261, 2628
StyI CCWWGG 2 cut(s) 87, 3016
TaaI ACNGT 7 cut(s) 145, 579, 1031, 1645, 1672, 1813, 2085
TaiI ACGT 4 cut(s) 161, 483, 1733, 3493
TaqI TCGA 8 cut(s) 32, 51, 269, 706, 1387, 1635, 1837, 2859
TatI WGTACW 4 cut(s) 1288, 1555, 2071, 2257
TauI GCSGC 4 cut(s) 209, 212, 281, 317
TscAI CASTG 7 cut(s) 288, 560, 622, 1650, 2449, 3370, 3544
TseFI GTSAC 3 cut(s) 700, 2008, 3411
Tsp45I GTSAC 3 cut(s) 700, 2008, 3411
TspGWI ACGGA 2 cut(s) 189, 511
TspMI CCCGGG 1 cut(s) 229
TspRI CASTG 7 cut(s) 288, 560, 622, 1650, 2449, 3370, 3544
Tth111I GACNNNGTC 1 cut(s) 160
Van91I CCANNNNNTGG 1 cut(s) 2177
Vha464I CTTAAG 1 cut(s) 2750
VpaK11BI GGWCC 3 cut(s) 2111, 3353, 3664
VspI ATTAAT 1 cut(s) 1790
XapI RAATTY 7 cut(s) 770, 826, 1086, 2534, 2928, 3071, 3562
XceI RCATGY 3 cut(s) 1652, 1821, 1979
XcmI CCANNNNNNNNNTGG 3 cut(s) 352, 660, 2615
XhoI CTCGAG 1 cut(s) 705
XmaI CCCGGG 1 cut(s) 229
XmiI GTMKAC 1 cut(s) 501
XmnI GAANNNNTTC 1 cut(s) 1543
ZraI GACGTC 1 cut(s) 159
ZrmI AGTACT 1 cut(s) 1557
Zsp2I ATGCAT 1 cut(s) 2589
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.