Rh4DG001500

SH3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0004649
Physical Location & Seq
Forward (+)
300837 .. 321283
20447 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG001500.1

Sequence Viewer

Length: 3804 bp
ATGGGCCCCACATTTGCCCCCAAGAAAAAGAAAAAGAAAAAAAAAAAAAATGTTGAGAATTGCCGAGCAGTGCTGCTGGCTGCATATCTCAAGATTGGCAGTCAGTCACCCATAACTAAAAACTTAAATTCTGACTCTCTCTCCCCAAGCTTAAGCTTAAGCTTAGGATTAGCCATGGCGGACTCGTCAGGGACGACCCTGATGGATCTGATAACGGCGGACCCTTCCACAGTATCAGCTTCTTCCACCTCCTCAACGACGTCGTCTTCGTCTTCGTCTTCCGCGCCTCCTCCTCCTCCTCCTTATGCGGCTTCACCCCCACCGCCCAGAGGCACCAGCCCCGGCTCCGCTCTCGGAAAGCCTGCTGTCGAGAAGAGGTCCAAGAGGGCTGCGCTTATGCAGATCCAGAACGACACCATCTCTGCCGCCAAAGCTGCTCTCAATCCCGTTCGCACCAACATCATCATGCCCCAGAAGCACAGGCACAAGCAGAAAAAGCCTGTTTCATATGCTCAACTTGCAAGGAGCATTCACGAACTAGCTGCTTCCTCCGATCAGAAAAGCTCCCAGAAGCAGTTGGTGAATCACGTCTTTCCCAAGCTCGCCGTCTACAATTCTGTTGACCCCTCTGTCGCCCCTTCTCTTCTCATGCTCAATCAGCAGTGTGAGGATAAGAGCGTTCTGCGATATGTCTATTATTACTTGGCCAGGATTTTATCAGATACTGGTGCACAAGGTGTAACCACAGGCGGTGGGATCCCAACTCCAAACTGGGATGCTCTAGCTGACATTGATGCTGTTGGAGGGGTAACTAGAGCTGATGTTGTACCAAGAATAGTAAATCAGCTCACTATAGAGGCTTCAAATGCTGATGCAGAATTTCATGCACGAAGACTGCAAGCTTTGAAGGCTCTTACATATGCTCCATCAACAAATTCTGAAATTTTGTCCAAATTGTATGAAATTGTGTTTGGCATTCTTGATAAGGTTGCTGATGGCCCACAAAAACGCAAGAAAGGTGTGTTTGGGACTAAAGGTGGCGATAAAGAGTTTATCATCCGAAGTAACTTGCAATATGCTGCCCTAAGTGCTCTTAGAAGACTTCCATTGGATCCAGGAAACCCAGCTTTTTTGTACCGTGCTGTTCAAGGGGTGTCATTTGCTGATCCTGTTGCTGTGAGGCATGCTTTGGAAATTCTGTCTGAGTTAGCCACAAAAGACCCTTATGCAGTTGTGATGGGCTTAGGGAAACATGCAGAGCCTGGAGGGGCTTTGCAGGATGTTCTCCACTTGCATGATGTTCTTGCTAGAGTTGCTCTAGCTAAGTTGTGTTATACAATTTCAAGAGCGAGAGCATTGGATGACAGGCCTGATATTAGATCTCAGTTCAACTCTGTACTCTATCAACTCCTCCTGGATCCCAGCGAAAGAGTCTGCTTTGAGGCAATCTTATGTATATTGGGAAAACATGATAACACAGAAAGGACTGATGATCGAGCTGCTGGGTGGTACCGATTAACTAGAGAGATTCTCAAGTTGCCAGAAGCACCTTCTGTGAAGGATTCCTCCAAAGATAAAGTTCAAAAGACGAGGCGTCCACAGCCCCTTATTAAACTTGTAATGAGAAGATTGGAGAGTTCTTTCAGAAGTTTTTCTAGGCCAGTACTCCATGCAGCATCAAGAGTTGTGCAGGAGATGGGAAAGAGTCGGGCTGCTGCATTTGCTCTAGGCATACAGGATATCGACGAATCAGTGCATGTTAATACATTTTCTGAGACTCTTGATTCACGCGAGATAGATGGTAGTGAGGCCTCACATCCTGAGAGTATCCGAAGAACCTCTTCCTTACCTACAGGGGTAGGTGGCAAGGATACAATAGCTAGTTTGTTAGCTTCATTAATGGAAGTTGTGCGGACAACTGTGGCATGCGAGTGTGTTTATGTTCGAGCAATGGTTATTAAGGCATTGATCTGGATGCAAAGTCCGCATGATTCGTTTGATCAACTAGAATCTATAATTGCATCAGAGCTTTCTGATCCAGCTTGGCCAGCCACGCTCTTGAATGATATTTTACTCACTTTGCATGCTCGGTTTAAGGCAACTCCAGATATGGCCGTTACTCTTCTTGAAATTGCAAGGATCTTTGCAACTAAAGCCCCCGGGAAGATCGATGCTGATGTACTACAACTACTGTGGAAGACTTGTCTTGTTGGAGCTGGTCCTGATGGGAAACACACTGCATTAGAAGCGGTTACCATAGTTCTCGATTTACCACCACCACAACCTGGGTCAATGTTGGGCCTTACATCAGTTGACAGGGTATCTGCATCTGATCCAAAGGCAGCCCTGGCCTTACAGAGATTAGTACAAGCAGCAGTATGGTTCCTTGGAGAAAATGCAAATTATGCTGCTTCTGAATATGCTTGGGAATCTGCAACCCCTCCTGGTACCGCATTGATGATGCTAGATACAGATAAAATGGTTGCTGCTGCCAGTTCCCGCAACCCTACTCTAGCTGGTGCATTGACTAGGCTACAGAGGTGTGCATTCAGTGGCAGCTGGGAGGTTCGGATCATTGCTGCTCAAGCTCTCACAACTATGGCAATAAGATCTGGTGAGCCTTTTAGGCTACAAATCTATGAATTTTTACACACTATAGCAGAGGGTGGAGTGCAGTCTCAGTTTTCTGAGATGCATCCCAGTAATGGAGAAGACCAGGGAGCTAGTGGCACAGGCCTCGGAGTTCTAATCAGTCCCATGATAGAGGTTCTTGATGAAATGTATAGGGCACAAGATGATTTGATCAAGGAAATGCGCAACCATGATAATGCTAATAAGGAATGGACAGATGAAGAGCTTAAGAAACTCTATGAAACCCATGAAAGGTTGCTTGATCTTGTTTCTCTGTTTTGTTATGTTCCAAGAGCAAAATATCTGCCTTTGGGGCCAATAAGTGCAAAACTTATCGACATCTATCGCACTAGGCACAATATTAGTCCATCAACTGGTTTGAGTGATCCAGCTGTTGCTACTGGAATTTCTGATCTTATGTATGAATCTAAACCAGCAGCTGTAGAGCCTGATGCGCTTGATGATGACCTTGTAAATGCTTGGGCAGCAAACCTTGGTGATGACGGACTGTTAGGAAATAATGCACCAGCAATGAGCAGGGTTAATGAATTTCTAGCCGGTGCTGGAACTGATGCTCCTGATGTTGATGAGGAAAACATCATCTCTAGGCCGTCAGTTGGTTACGATGATATGTGGGCAAAGACCCTATTAGAGACTTCAGAACTGGAGGAAGAAGACGCGAGGTCATCTGGGACATCTTCACCTGAATCAACAGGCTCGGTCGAAACTTCCATATCATCGCATTTTGGGGGAATGAATTACCCTTCACTTTTCAGTTCACGGCCGGAGAGGTCTGGAGGAAGCAGATATAGCAATCCATCTATGGGGGGTCCCTCATTCAGTGAGGGTTTAGGATCTCCAATTAGAGAAGAGCCTCCGCCATACTCGTCACCTGCAACGCAAAGATTTGAGTCGTTTGAGAATCCCTTAGCCCCCAGATCCCAGAGTTTTGGATCCCAAGATGACGAACGCGTTTCTTCTGGAAATCCTCAACATGGAACAGCACTTTATGACTTCACTGCTGGTGGAGATGATGAGTTAAATTTAACAGCTGGAGAGGAGGTTGATATCGAGTACGAAGTGGATGGCTGGTTTTATGTAAAGAAGAAACGCCCTGGGAGGGATGGGAAAATGGCTGGTCTGGTCCCAGTCCTCTATGTTAATCAGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SH3_1 SH3_9

Protein Analysis

1267

Amino Acids

137.07

Weight (kDa)

5.85

Isoelectric Point (pI)

49.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SH3_1 PF00018 1211 - 1252 7.6e-06 SH3 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 3532
AasI GACNNNNNNGTC 1 cut(s) 629
AatII GACGTC 1 cut(s) 263
Acc16I TGCGCA 1 cut(s) 2816
Acc36I ACCTGC 1 cut(s) 3532
Acc65I GGTACC 2 cut(s) 1509, 2447
AccB1I GGYRCC 3 cut(s) 332, 1509, 2447
AccB7I CCANNNNNTGG 2 cut(s) 2285, 3005
AccBSI CCGCTC 1 cut(s) 350
AccI GTMKAC 1 cut(s) 609
AccII CGCG 4 cut(s) 284, 1791, 3311, 3603
AcoI YGGCCR 4 cut(s) 705, 2045, 2112, 3413
AcsI RAATTY 9 cut(s) 127, 878, 934, 942, 1194, 2642, 3036, 3179, 3673
AcuI CTGAAG 1 cut(s) 3273
AcyI GRCGYC 2 cut(s) 260, 1594
AdeI CACNNNGTG 1 cut(s) 737
AfaI GTAC 9 cut(s) 828, 1136, 1398, 1511, 1665, 2181, 2367, 2449, 3707
AfiI CCNNNNNNNGG 9 cut(s) 329, 2285, 2705, 2883, 3005, 3248, 3455, 3626, 3751
AflII CTTAAG 3 cut(s) 151, 157, 2858
AflIII ACRYGT 1 cut(s) 3601
AgsI TTSAA 8 cut(s) 864, 907, 1148, 1344, 1390, 1583, 2062, 2129
AhdI GACNNNNNGTC 2 cut(s) 1593, 3313
AjiI CACGTC 1 cut(s) 589
AjnI CCWGG 9 cut(s) 707, 1114, 1261, 1413, 2284, 2346, 2443, 2715, 3745
AjuI GAANNNNNNNTTGG 2 cut(s) 954, 986
AloI GAACNNNNNNTCC 2 cut(s) 3190, 3222
Alw21I GWGCWC 2 cut(s) 733, 1093
Alw26I GTCTC 3 cut(s) 1769, 2682, 3278
Alw44I GTGCAC 1 cut(s) 729
AlwNI CAGNNNCTG 3 cut(s) 725, 1262, 3071
Ama87I CYCGRG 1 cut(s) 2159
ApaI GGGCCC 1 cut(s) 8
ApaLI GTGCAC 1 cut(s) 729
ApoI RAATTY 9 cut(s) 127, 878, 934, 942, 1194, 2642, 3036, 3179, 3673
ArsI GACNNNNNNTTYG 2 cut(s) 250, 282
AseI ATTAAT 1 cut(s) 1898
Asp700I GAANNNNTTC 2 cut(s) 1651, 1840
Asp718I GGTACC 2 cut(s) 1509, 2447
AspLEI GCGC 4 cut(s) 286, 394, 2817, 3088
AsuC2I CCSGG 3 cut(s) 342, 2160, 2161
AsuHPI GGTGA 7 cut(s) 99, 306, 592, 2627, 3140, 3324, 3513
AvaI CYCGRG 1 cut(s) 2159
AvaII GGWCC 5 cut(s) 220, 378, 2219, 3461, 3775
BaeGI GKGCMC 3 cut(s) 8, 733, 2791
BalI TGGCCA 2 cut(s) 707, 2047
BamHI GGATCC 4 cut(s) 756, 1111, 1417, 3584
BanI GGYRCC 3 cut(s) 332, 1509, 2447
BanII GRGCYC 1 cut(s) 8
BbsI GAAGAC 8 cut(s) 258, 264, 270, 898, 1105, 2204, 2718, 3312
Bbv12I GWGCWC 2 cut(s) 733, 1093
BceAI ACGGC 5 cut(s) 231, 590, 2099, 3226, 3428
BcgI CGANNNNNNTGC 4 cut(s) 524, 558, 2719, 2753
BciT130I CCWGG 9 cut(s) 709, 1116, 1263, 1415, 2286, 2348, 2445, 2717, 3747
BciVI GTATCC 2 cut(s) 1838, 1864
BclI TGATCA 2 cut(s) 1999, 2802
BcnI CCSGG 3 cut(s) 342, 2160, 2161
BcoDI GTCTC 3 cut(s) 1769, 2682, 3278
BfmI CTRYAG 5 cut(s) 852, 1851, 2534, 2655, 3072
BfrI CTTAAG 3 cut(s) 151, 157, 2858
BfuAI ACCTGC 1 cut(s) 3532
BfuI GTATCC 2 cut(s) 1838, 1864
BglI GCCNNNNNGGC 2 cut(s) 2626, 2944
BglII AGATCT 2 cut(s) 1379, 2609
BmcAI AGTACT 1 cut(s) 1665
Bme18I GGWCC 5 cut(s) 220, 378, 2219, 3461, 3775
BmeRI GACNNNNNGTC 2 cut(s) 1593, 3313
BmeT110I CYCGRG 1 cut(s) 2159
BmgBI CACGTC 1 cut(s) 589
BmrI ACTGGG 3 cut(s) 781, 2694, 3773
BmuI ACTGGG 3 cut(s) 781, 2694, 3773
BpiI GAAGAC 8 cut(s) 258, 264, 270, 898, 1105, 2204, 2718, 3312
BplI GAGNNNNNCTC 2 cut(s) 1515, 1547
BpmI CTGGAG 5 cut(s) 1284, 2088, 3317, 3447, 3705
Bpu10I CCTNAGC 3 cut(s) 163, 1243, 3559
BpuEI CTTGAG 3 cut(s) 74, 1517, 2568
BpuMI CCSGG 3 cut(s) 342, 2160, 2161
Bsa29I ATCGAT 1 cut(s) 2169
BsaHI GRCGYC 2 cut(s) 260, 1594
BsaXI ACNNNNNCTCC 8 cut(s) 125, 155, 907, 937, 3190, 3220, 3678, 3708
Bsc4I CCNNNNNNNGG 9 cut(s) 329, 2285, 2705, 2883, 3005, 3248, 3455, 3626, 3751
Bse118I RCCGGY 1 cut(s) 3188
Bse1I ACTGG 9 cut(s) 730, 776, 1661, 2493, 2700, 3010, 3037, 3300, 3779
Bse3DI GCAATG 3 cut(s) 1956, 2574, 3168
BseBI CCWGG 9 cut(s) 709, 1116, 1263, 1415, 2286, 2348, 2445, 2717, 3747
BseCI ATCGAT 1 cut(s) 2169
BseGI GGATG 9 cut(s) 781, 1056, 1285, 1366, 1816, 1980, 2695, 3721, 3760
BseLI CCNNNNNNNGG 9 cut(s) 329, 2285, 2705, 2883, 3005, 3248, 3455, 3626, 3751
BseMI GCAATG 3 cut(s) 1956, 2574, 3168
BseMII CTCAG 6 cut(s) 1194, 1397, 1764, 1812, 2679, 2693
BseNI ACTGG 9 cut(s) 730, 776, 1661, 2493, 2700, 3010, 3037, 3300, 3779
BseRI GAGGAG 7 cut(s) 241, 279, 282, 285, 288, 1400, 3704
BseSI GKGCMC 3 cut(s) 8, 733, 2791
BseX3I CGGCCG 1 cut(s) 3413
BseYI CCCAGC 4 cut(s) 1123, 1421, 1502, 2559
BsgI GTGCAG 2 cut(s) 1709, 2693
Bsh1236I CGCG 4 cut(s) 284, 1791, 3311, 3603
Bsh1285I CGRYCG 2 cut(s) 3354, 3416
BshNI GGYRCC 3 cut(s) 332, 1509, 2447
BshVI ATCGAT 1 cut(s) 2169
BsiEI CGRYCG 2 cut(s) 3354, 3416
BsiHKAI GWGCWC 2 cut(s) 733, 1093
BsiHKCI CYCGRG 1 cut(s) 2159
BsiSI CCGG 4 cut(s) 342, 2160, 3189, 3416
BslFI GGGAC 6 cut(s) 205, 1042, 2739, 3337, 3447, 3761
BslI CCNNNNNNNGG 9 cut(s) 329, 2285, 2705, 2883, 3005, 3248, 3455, 3626, 3751
BsmAI GTCTC 3 cut(s) 1769, 2682, 3278
BsmFI GGGAC 6 cut(s) 205, 1042, 2739, 3337, 3447, 3761
BsmI GAATGC 3 cut(s) 528, 975, 2546
BsoBI CYCGRG 1 cut(s) 2159
Bsp120I GGGCCC 1 cut(s) 4
Bsp1286I GDGCHC 4 cut(s) 8, 733, 1093, 2791
Bsp19I CCATGG 1 cut(s) 174
BspCNI CTCAG 6 cut(s) 1195, 1396, 1765, 1813, 2680, 2692
BspDI ATCGAT 1 cut(s) 2169
BspFNI CGCG 4 cut(s) 284, 1791, 3311, 3603
BspMI ACCTGC 1 cut(s) 3532
BspQI GCTCTTC 2 cut(s) 2847, 3495
BspT107I GGYRCC 3 cut(s) 332, 1509, 2447
BspTI CTTAAG 3 cut(s) 151, 157, 2858
BsrBI CCGCTC 1 cut(s) 350
BsrDI GCAATG 3 cut(s) 1956, 2574, 3168
BsrFI RCCGGY 1 cut(s) 3188
BsrI ACTGG 9 cut(s) 730, 776, 1661, 2493, 2700, 3010, 3037, 3300, 3779
BssAI RCCGGY 1 cut(s) 3188
BssNI GRCGYC 2 cut(s) 260, 1594
BssT1I CCWWGG 3 cut(s) 174, 2386, 3124
Bst2UI CCWGG 9 cut(s) 709, 1116, 1263, 1415, 2286, 2348, 2445, 2717, 3747
Bst4CI ACNGT 5 cut(s) 232, 1139, 1921, 2193, 3141
Bst6I CTCTTC 6 cut(s) 368, 648, 1846, 2127, 2847, 3495
BstACI GRCGYC 2 cut(s) 260, 1594
BstAFI CTTAAG 3 cut(s) 151, 157, 2858
BstAPI GCANNNNNTGC 1 cut(s) 2405
BstC8I GCNNGC 8 cut(s) 78, 363, 603, 900, 1185, 1927, 2049, 2085
BstDSI CCRYGG 1 cut(s) 174
BstEII GGTNACC 1 cut(s) 2251
BstF5I GGATG 9 cut(s) 781, 1056, 1285, 1366, 1816, 1980, 2695, 3721, 3760
BstFNI CGCG 4 cut(s) 284, 1791, 3311, 3603
BstHHI GCGC 4 cut(s) 286, 394, 2817, 3088
BstMAI GTCTC 3 cut(s) 1769, 2682, 3278
BstMCI CGRYCG 2 cut(s) 3354, 3416
BstNI CCWGG 9 cut(s) 709, 1116, 1263, 1415, 2286, 2348, 2445, 2717, 3747
BstNSI RCATGY 5 cut(s) 1187, 1256, 1760, 1929, 2087
BstPI GGTNACC 1 cut(s) 2251
BstSFI CTRYAG 5 cut(s) 852, 1851, 2534, 2655, 3072
BstSLI GKGCMC 3 cut(s) 8, 733, 2791
BstUI CGCG 4 cut(s) 284, 1791, 3311, 3603
BstV2I GAAGAC 8 cut(s) 258, 264, 270, 898, 1105, 2204, 2718, 3312
BstXI CCANNNNNNTGG 1 cut(s) 3581
BstZI CGGCCG 1 cut(s) 3413
Bsu15I ATCGAT 1 cut(s) 2169
BsuI GTATCC 2 cut(s) 1838, 1864
BsuTUI ATCGAT 1 cut(s) 2169
BtgI CCRYGG 1 cut(s) 174
BtgZI GCGATG 1 cut(s) 3354
BtrI CACGTC 1 cut(s) 589
BtsCI GGATG 9 cut(s) 781, 1056, 1285, 1366, 1816, 1980, 2695, 3721, 3760
BtsI GCAGTG 4 cut(s) 75, 668, 2235, 3648
BtsIMutI CAGTG 7 cut(s) 75, 668, 1758, 2235, 2557, 3478, 3648
BveI ACCTGC 1 cut(s) 3532
Cac8I GCNNGC 8 cut(s) 78, 363, 603, 900, 1185, 1927, 2049, 2085
CaiI CAGNNNCTG 3 cut(s) 725, 1262, 3071
CfoI GCGC 4 cut(s) 286, 394, 2817, 3088
Cfr10I RCCGGY 1 cut(s) 3188
Cfr9I CCCGGG 1 cut(s) 2159
ClaI ATCGAT 1 cut(s) 2169
CseI GACGC 2 cut(s) 1583, 3317
Csp6I GTAC 9 cut(s) 827, 1135, 1397, 1510, 1664, 2180, 2366, 2448, 3706
CspCI CAANNNNNGTGG 2 cut(s) 2174, 2209
CviQI GTAC 9 cut(s) 827, 1135, 1397, 1510, 1664, 2180, 2366, 2448, 3706
DraIII CACNNNGTG 1 cut(s) 737
DrdI GACNNNNNNGTC 1 cut(s) 629
DriI GACNNNNNGTC 2 cut(s) 1593, 3313
DseDI GACNNNNNNGTC 1 cut(s) 629
EaeI YGGCCR 4 cut(s) 705, 2045, 2112, 3413
EagI CGGCCG 1 cut(s) 3413
Eam1104I CTCTTC 6 cut(s) 368, 648, 1846, 2127, 2847, 3495
Eam1105I GACNNNNNGTC 2 cut(s) 1593, 3313
EarI CTCTTC 6 cut(s) 368, 648, 1846, 2127, 2847, 3495
EciI GGCGGA 3 cut(s) 194, 233, 3498
EclXI CGGCCG 1 cut(s) 3413
Eco130I CCWWGG 3 cut(s) 174, 2386, 3124
Eco147I AGGCCT 3 cut(s) 1369, 1811, 2736
Eco24I GRGCYC 1 cut(s) 8
Eco32I GATATC 2 cut(s) 1741, 3700
Eco47I GGWCC 5 cut(s) 220, 378, 2219, 3461, 3775
Eco52I CGGCCG 1 cut(s) 3413
Eco57I CTGAAG 1 cut(s) 3273
Eco88I CYCGRG 1 cut(s) 2159
Eco91I GGTNACC 1 cut(s) 2251
EcoO109I RGGNCCY 2 cut(s) 5, 3461
EcoO65I GGTNACC 1 cut(s) 2251
EcoRII CCWGG 9 cut(s) 707, 1114, 1261, 1413, 2284, 2346, 2443, 2715, 3745
EcoRV GATATC 2 cut(s) 1741, 3700
EcoT14I CCWWGG 3 cut(s) 174, 2386, 3124
EcoT22I ATGCAT 1 cut(s) 2697
EcoT38I GRGCYC 1 cut(s) 8
ErhI CCWWGG 3 cut(s) 174, 2386, 3124
FalI AAGNNNNNCTT 2 cut(s) 1825, 1857
FaqI GGGAC 6 cut(s) 205, 1042, 2739, 3337, 3447, 3761
FauI CCCGC 1 cut(s) 2507
FauNDI CATATG 2 cut(s) 508, 919
FbaI TGATCA 2 cut(s) 1999, 2802
FblI GTMKAC 1 cut(s) 609
FokI GGATG 9 cut(s) 788, 1043, 1292, 1373, 1803, 1987, 2682, 3728, 3767
FriOI GRGCYC 1 cut(s) 8
FspI TGCGCA 1 cut(s) 2816
GlaI GCGC 4 cut(s) 285, 393, 2816, 3087
GsaI CCCAGC 4 cut(s) 1127, 1425, 1506, 2563
GsuI CTGGAG 5 cut(s) 1284, 2088, 3317, 3447, 3705
HapII CCGG 4 cut(s) 342, 2160, 3189, 3416
HgaI GACGC 2 cut(s) 1583, 3317
HhaI GCGC 4 cut(s) 286, 394, 2817, 3088
Hin1I GRCGYC 2 cut(s) 260, 1594
Hin6I GCGC 4 cut(s) 284, 392, 2815, 3086
HinP1I GCGC 4 cut(s) 284, 392, 2815, 3086
HincII GTYRAC 2 cut(s) 622, 2314
HindII GTYRAC 2 cut(s) 622, 2314
HindIII AAGCTT 4 cut(s) 148, 154, 160, 900
HpaII CCGG 4 cut(s) 342, 2160, 3189, 3416
HphI GGTGA 7 cut(s) 99, 306, 592, 2627, 3140, 3324, 3513
Hpy166II GTNNAC 6 cut(s) 610, 622, 731, 1598, 2314, 3410
Hpy8I GTNNAC 6 cut(s) 610, 622, 731, 1598, 2314, 3410
Hpy99I CGWCG 3 cut(s) 262, 265, 1748
HpyAV CCTTC 6 cut(s) 234, 648, 901, 1552, 1560, 3405
HpyCH4III ACNGT 5 cut(s) 232, 1139, 1921, 2193, 3141
HpyCH4IV ACGT 2 cut(s) 260, 588
HpySE526I ACGT 2 cut(s) 260, 588
Hsp92I GRCGYC 2 cut(s) 260, 1594
HspAI GCGC 4 cut(s) 284, 392, 2815, 3086
KflI GGGWCCC 1 cut(s) 3461
KpnI GGTACC 2 cut(s) 1513, 2451
Ksp22I TGATCA 2 cut(s) 1999, 2802
LguI GCTCTTC 2 cut(s) 2847, 3495
LmnI GCTCC 7 cut(s) 350, 525, 569, 928, 2213, 2720, 3211
MaeII ACGT 2 cut(s) 260, 588
MaeIII GTNAC 8 cut(s) 105, 739, 808, 1064, 2116, 2251, 3251, 3519
MbiI CCGCTC 1 cut(s) 350
MhlI GDGCHC 4 cut(s) 8, 733, 1093, 2791
MlsI TGGCCA 2 cut(s) 707, 2047
MluI ACGCGT 1 cut(s) 3601
MluNI TGGCCA 2 cut(s) 707, 2047
MlyI GAGTC 6 cut(s) 128, 176, 1440, 1714, 1771, 3551
MmeI TCCRAC 2 cut(s) 781, 2191
Mox20I TGGCCA 2 cut(s) 707, 2047
Mph1103I ATGCAT 1 cut(s) 2697
MroXI GAANNNNTTC 2 cut(s) 1651, 1840
MscI TGGCCA 2 cut(s) 707, 2047
MslI CAYNNNNRTG 5 cut(s) 464, 1293, 1930, 2597, 2826
Msp20I TGGCCA 2 cut(s) 707, 2047
MspA1I CMGCKG 4 cut(s) 2559, 3023, 3071, 3683
MspCI CTTAAG 3 cut(s) 151, 157, 2858
MspI CCGG 4 cut(s) 342, 2160, 3189, 3416
Mva1269I GAATGC 3 cut(s) 528, 975, 2546
MvaI CCWGG 9 cut(s) 709, 1116, 1263, 1415, 2286, 2348, 2445, 2717, 3747
MvnI CGCG 4 cut(s) 284, 1791, 3311, 3603
NciI CCSGG 3 cut(s) 342, 2160, 2161
NcoI CCATGG 1 cut(s) 174
NdeI CATATG 2 cut(s) 508, 919
NmeAIII GCCGAG 1 cut(s) 89
NmuCI GTSAC 2 cut(s) 105, 3519
NsbI TGCGCA 1 cut(s) 2816
NsiI ATGCAT 1 cut(s) 2697
NspI RCATGY 5 cut(s) 1187, 1256, 1760, 1929, 2087
PaeI GCATGC 3 cut(s) 1187, 1929, 2087
PaqCI CACCTGC 1 cut(s) 3532
PasI CCCWGGG 1 cut(s) 3746
PceI AGGCCT 3 cut(s) 1369, 1811, 2736
PciSI GCTCTTC 2 cut(s) 2847, 3495
PcsI WCGNNNNNNNCGW 2 cut(s) 191, 266
PctI GAATGC 3 cut(s) 528, 975, 2546
PdmI GAANNNNTTC 2 cut(s) 1651, 1840
PflFI GACNNNGTC 1 cut(s) 262
PflMI CCANNNNNTGG 2 cut(s) 2285, 3005
PfoI TCCNGGA 2 cut(s) 1114, 1413
PleI GAGTC 6 cut(s) 128, 176, 1439, 1713, 1771, 3550
PpsI GAGTC 6 cut(s) 128, 176, 1439, 1713, 1771, 3550
PpuMI RGGWCCY 1 cut(s) 3461
PshBI ATTAAT 1 cut(s) 1898
Psp5II RGGWCCY 1 cut(s) 3461
Psp6I CCWGG 9 cut(s) 707, 1114, 1261, 1413, 2284, 2346, 2443, 2715, 3745
PspEI GGTNACC 1 cut(s) 2251
PspFI CCCAGC 4 cut(s) 1123, 1421, 1502, 2559
PspGI CCWGG 9 cut(s) 707, 1114, 1261, 1413, 2284, 2346, 2443, 2715, 3745
PspOMI GGGCCC 1 cut(s) 4
PspPPI RGGWCCY 1 cut(s) 3461
PstNI CAGNNNCTG 3 cut(s) 725, 1262, 3071
PsyI GACNNNGTC 1 cut(s) 262
PvuII CAGCTG 4 cut(s) 2559, 3023, 3071, 3683
RsaI GTAC 9 cut(s) 828, 1136, 1398, 1511, 1665, 2181, 2367, 2449, 3707
RsaNI GTAC 9 cut(s) 827, 1135, 1397, 1510, 1664, 2180, 2366, 2448, 3706
RseI CAYNNNNRTG 5 cut(s) 464, 1293, 1930, 2597, 2826
SapI GCTCTTC 2 cut(s) 2847, 3495
ScaI AGTACT 1 cut(s) 1665
SchI GAGTC 6 cut(s) 128, 176, 1440, 1714, 1771, 3551
SduI GDGCHC 4 cut(s) 8, 733, 1093, 2791
SfcI CTRYAG 5 cut(s) 852, 1851, 2534, 2655, 3072
SinI GGWCC 5 cut(s) 220, 378, 2219, 3461, 3775
SmaI CCCGGG 1 cut(s) 2161
SmiMI CAYNNNNRTG 5 cut(s) 464, 1293, 1930, 2597, 2826
SmlI CTYRAG 6 cut(s) 89, 151, 157, 1532, 2583, 2858
SmoI CTYRAG 6 cut(s) 89, 151, 157, 1532, 2583, 2858
SphI GCATGC 3 cut(s) 1187, 1929, 2087
SseBI AGGCCT 3 cut(s) 1369, 1811, 2736
SspI AATATT 1 cut(s) 2992
StuI AGGCCT 3 cut(s) 1369, 1811, 2736
StyI CCWWGG 3 cut(s) 174, 2386, 3124
TaaI ACNGT 5 cut(s) 232, 1139, 1921, 2193, 3141
TaiI ACGT 2 cut(s) 263, 591
TaqI TCGA 9 cut(s) 369, 1495, 1743, 1945, 2169, 2265, 2967, 3354, 3702
TaqII GACCGA 1 cut(s) 3340
TatI WGTACW 4 cut(s) 1396, 1663, 2179, 2365
TauI GCSGC 2 cut(s) 311, 428
TscAI CASTG 7 cut(s) 75, 668, 1758, 2242, 2557, 3478, 3655
TseFI GTSAC 2 cut(s) 105, 3519
Tsp45I GTSAC 2 cut(s) 105, 3519
TspGWI ACGGA 1 cut(s) 3150
TspMI CCCGGG 1 cut(s) 2159
TspRI CASTG 7 cut(s) 75, 668, 1758, 2242, 2557, 3478, 3655
Tth111I GACNNNGTC 1 cut(s) 262
Van91I CCANNNNNTGG 2 cut(s) 2285, 3005
Vha464I CTTAAG 3 cut(s) 151, 157, 2858
VneI GTGCAC 1 cut(s) 729
VpaK11BI GGWCC 5 cut(s) 220, 378, 2219, 3461, 3775
VspI ATTAAT 1 cut(s) 1898
XapI RAATTY 9 cut(s) 127, 878, 934, 942, 1194, 2642, 3036, 3179, 3673
XceI RCATGY 5 cut(s) 1187, 1256, 1760, 1929, 2087
XcmI CCANNNNNNNNNTGG 2 cut(s) 768, 2723
XmaI CCCGGG 1 cut(s) 2159
XmiI GTMKAC 1 cut(s) 609
XmnI GAANNNNTTC 2 cut(s) 1651, 1840
ZraI GACGTC 1 cut(s) 261
ZrmI AGTACT 1 cut(s) 1665
Zsp2I ATGCAT 1 cut(s) 2697
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.